FOOD CHEMISTRY
·
2020
·
IF 6.306
Yuning Yang,Wei Liu,Jie Cao,Yiwei Wu
A ratiometric sensor of MOF/ CdTe QDs was constructed by physically mixing CdTe QDs ( λ em = 605 nm) with MOF (Fe-MIL-88NH 2, λ em = 425 nm). Under an UV lamp ( λ ex = 365 nm), orange-red CdTe QDs is gradually quenched with increasing levels of Hg 2+ or Cu 2+ , while blue MOF is unchanged, accompanying with a visual change from bright pink to blue fluorescence. Hence, a new method for determining Hg 2+ and Cu 2+ by MOF/CdTe QDs sensor is established, whose detection limits are 0.22 ng mL −1 for Hg 2+ (2.1% RSD) and 0.26 ng mL −1 for Cu 2+ (1.1% RSD), respectively. In addition, on-site, rapid and visual determination of Hg 2+ /Cu 2+ in red wine without any pretreatment is realized by using a MOF/CdTe QDs paper-based sensor. For Hg 2+ and Cu 2+ , the semi-quantitative detection ranges of the paper-based sensor are both 10 ~ 5000 ng mL −1 , implying that it has great application value in analyzing Hg 2+ and Cu 2+ in liquid samples.
研究领域:
分析化学
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材料科学
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环境科学
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2022
·Vol. 23
·期 11
·6326
·
IF 6.208
Ting Sun,Xian Li,Xiaochuan Jin,Ziyi Wu,Xiachao Chen,Jieqiong Qiu
Graphene oxide is well known for its excellent fluorescence quenching ability. In this study, positively charged graphene oxide (pGO25000) was developed as a fluorescence quencher that is water-soluble and synthesized by grafting polyetherimide onto graphene oxide nanosheets by a carbodiimide reaction. Compared to graphene oxide, the fluorescence quenching ability of pGO25000 is significantly improved by the increase in the affinity between pGO25000 and the DNA strand, which is introduced by the additional electrostatic interaction. The FAM-labeled single-stranded DNA probe can be almost completely quenched at concentrations of pGO25000 as low as 0.1 μg/mL. A simple and novel FAM-labeled single-stranded DNA sensor was designed for Hg2+detection to take advantage of exonuclease I-triggered single-stranded DNA hydrolysis, and pGO25000 acted as a fluorescence quencher. The FAM-labeled single-stranded DNA probe is present as a hairpin structure by the formation of T–Hg2+–T when Hg2+is present, and no fluorescence is observed. It is digested by exonuclease I without Hg2+, and fluorescence is recovered. The fluorescence intensity of the proposed biosensor was positively correlated with the Hg2+concentration in the range of 0–250 nM (R2= 0.9955), with a seasonable limit of detection (3σ) cal. 3.93 nM. It was successfully applied to real samples of pond water for Hg2+detection, obtaining a recovery rate from 99.6% to 101.1%.Keywords:positively charged graphene oxide (pGO);exonuclease I;fluorescence quencher;hairpin structure;T–Hg2+–T
研究领域:
生物化学
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环境科学
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产品关联
DOI
PubMed
高影响因子
ANALYTICAL SCIENCES
·
2016
·Vol. 32
·期 2
·161-166
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IF 1.174
Xiaolin GUAN,Hongting FAN,Yang ZHANG,Donghai ZHANG,Tianming JIA,Shoujun LAI,Ziqiang LEI
Using environment-friendly materials for sensing toxic metal ions has drawn significant attention in recent research. Herein, we present an aqueous synthesis of stable CdS quantum dots (QDs) using thiol-functionalized poly(vinyl alcohol) (PVA) as the unique capping ligand for the detection of trace Hg2+ in environmental water samples. The CdS QDs with an average size of 3.3 nm had good water-solubility and favorable fluorescence with a quantum yield of 32.8% and a longer luminescence lifetime of 31.9 ns. The fluorescence intensity of QDs aqueous solution in the 520 nm wavelength was quenched upon the addition of Hg2+. Under the optimal conditions, the ratio of the blank fluorescence intensity to the quenched fluorescence intensity was linearly proportional to the Hg2+ concentration from 2 to 4000 nM with a detection limit of 1 nM. Also, many co-existing metal ions were not interfered with the detection of Hg2+. This nanomaterial was successfully applied to the measurement of Hg2+ in water samples.
研究领域:
分析化学
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环境科学
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纳米技术
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产品关联
DOI
PubMed
高影响因子
Analytical Methods
·
2025
·
IF 2.6
Xinyan Liu,Chao Liu,Qingyue Hu,Huihui Ge,Jie Tan,Jianhua Wang,Wei Bian
It is important to be capable of achieving highly selective detection of Ag+ and Hg2+ because their excessive concentrations may pose a serious threat to human health and lead to a variety of diseases. Herein, a new type of orange fluorescent carbon dot with an emission wavelength of 590 nm was synthesized by a hydrothermal method using 1,2,4-triaminobenzene dihydrochloride and ethylenediamine as raw materials. The synthesized orange carbon dots have high affinity for Hg2+ and Ag+ ions, which can be selectively detected by fluorescence quenching with the detection limits of 0.37 nM and 0.65 nM, respectively. Moreover, the carbon dots have good fluorescence stability for the rapid detection of Ag+ and Hg2+ over a wide pH range. Importantly, the addition of EDTA reversibly restored the fluorescence intensity of the mixed solution of carbon dots and Hg2+, but not the fluorescence intensity of the Ag+-containing probe solution, which allowed for a clear distinction between Hg2+ and Ag+. In addition, the carbon dots have good biocompatibility and low toxicity to MCF-7 cells, which can be used for cell imaging and intracellular ion detection.
研究领域:
分析化学
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环境科学
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材料科学
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生物成像
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毒理学
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产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
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2018
·
IF 5.667
Bao-xing Shen,Ying Qian
A novel ratiometric fluorescent probe RhB-BODIPY-OH based on Rhodamine and boron dipyrromethene (BODIPY) platform for sensing Fe 3+ and Hg 2+ ions was synthesized by Click reaction. RhB-BODIPY-OH dendron was a hybrid fluorescent probe with reaction-based as well as lock/key-based detection sites, which contains four recognition positions including Schiff base C N functionality, triazole–triazole linker and the spirocyclic ring of the Rhodamine unit. Naked eye detection was realized with the obvious color change. In addition, low detection limit was calculated from titration results with the value were 7.74 × 10 −8 M for RhB-BODIPY-OH/Hg 2+ as well as 3.91 × 10 −7 M for RhB-BODIPY-OH/Fe 3+ , separately. The probe is highly sensitive, the detection time is less than 2s. On top of that, living cell imaging experiment was carried out, which demonstrated that RhB-BODIPY-OH is a good bioimaging agent in living cells. Eventually, amino-group-functionalized silica fluorescent nanoparticles (FNPs) encapsulating the RhB-BODIPY-OH dyes were prepared and characterized by transmission electron microscopy.
研究领域:
化学
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生物医学工程
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分析化学
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DOI
高影响因子
ANALYTICA CHIMICA ACTA
·
2019
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IF 5.256
Zhihong Li,Hongjing Sun,Xinyue Ma,Ruifang Su,Rui Sun,Chuanyu Yang,Chunyan Sun
A novel label-free fluorescence “turn-on” strategy was developed for the sensitive detection of Hg 2+ based on the thymine-Hg 2+ -thymine (T-Hg 2+ -T) coordination and the fact that single-stranded DNA (ssDNA) greatly enhances the fluorescence of terbium (III) (Tb 3+ ), but double-stranded DNA (dsDNA) does not. In the absence of Hg 2+ , the mercury-specific DNA (MSD) hybridized with the corresponding complementary strand (cDNA) to form a double helix structure in solution based on Watson-Crick base pairings, which cannot enhance the fluorescence of Tb 3+ . In the presence of Hg 2+ , MSD preferentially bound with Hg 2+ to form the T-Hg 2+ -T complex due to the strong affinity of Hg(II) for the T bases of DNA, thus avoiding the hybridization of the cDNA to MSD. The free cDNA can greatly enhance the emission of Tb 3+ , leading to a sharp increase in fluorescence intensity . Under the optimized conditions, the increased fluorescence intensity was proportional to the concentration of Hg 2+ in the range from 10 to 600 nM, and this method can detect concentrations of Hg 2+ as low as 0.24 nM. Moreover, satisfactory results were obtained for the detection of Hg 2+ in river water and fish samples, and the results were consistent with those from the atomic fluorescence spectroscopy (AFS). Thus, the fluorescence characteristics of Tb 3+ were here used in a “turn on” approach to detect Hg 2+ , which represents a new opportunity for Hg 2+ analysis in the field of environmental monitoring and food safety.
研究领域:
环境科学
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食品安全
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分析化学
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DOI
PubMed
高影响因子
MOLECULES
·
2023
·Vol. 28
·期 14
·5355
·
IF 4.6
Keyan Chen,Shiqi Fu,Chenyu Jin,Fan Guo,Yu He,Qi Ren,Xuesheng Wang
Smartphone-assisted fluorescence and colorimetric methods for the on-site detection of Hg2+and Cl−were established based on the oxidase-like activity of the Au–Hg alloy on the surface of Au/Cu/Ti3C2NSs. The Au nanoparticles (NPs) were constructed via in-situ growth on the surface of Cu/Ti3C2NSs and characterized by different characterization techniques. After the addition of Hg2+, the formation of Hg–Au alloys could promote the oxidization of o-phenylenediamine (OPD) to generate a new fluorescence emission peak of 2,3-diaminopenazine (ADP) at 570 nm. Therefore, a turn-on fluorescence method for the detection of Hg2+was established. As the addition of Cl−can influence the fluorescence of ADP, the fluorescence intensity was constantly quenched to achieve the continuous quantitative detection of Cl−. Therefore, a turn-off fluorescence method for the detection of Cl−was established. This method had good linear ranges for the detection of Hg2+and Cl−in 8.0–200.0 nM and 5.0–350.0 µM, with a detection limit of 0.8 nM and 27 nM, respectively. Depending on the color change with the detection of Hg2+and Cl−, a convenient on-site colorimetric method for an analysis of Hg2+and Cl−was achieved by using digital images combined with smartphones (color recognizers). The digital picture sensor could analyze RGB values in concentrations of Hg2+or Cl−via a smartphone app. In summary, the proposed Au/Cu/Ti3C2NSs-based method provided a novel and more comprehensive application for environmental monitoring.Keywords:Au/Cu/Ti3C2NS;Au–Hg alloy;oxidase-like activity;smartphone;colorimetric detection
研究领域:
环境科学
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分析化学
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材料科学
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纳米技术
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产品关联
DOI
PubMed
高影响因子
Journal of Environmental Chemical Engineering
·
2023
·
IF 7.7
Yu Gao,Linan Zhou,Zechang Li,Peng Sun,Qijun Dai,Ruifeng Xie,Fang Chai
Creating a sensitive, accuracy and portable fluorescent probe to monitor Hg 2+ with rapid visual convenient process is highly desirable but remains challenging. Herein, orange emissive carbon dots (O-CDs) were synthesized and optimized by solvothermal method using p-phenylenediamine (p-PDA) as precursor. The O-CDs emitted an orange emission at 594 nm excited at 480 nm, with QY of 23.25% and a long lifetime of 14.43 μs. The O-CDs exhibited sensitive quenching response towards Hg 2+ based on dynamic quenching effect, which facilitated O-CDs utilizing as the fluorescent probe with the limit of detection at 37.6 nM. The O-CDs fulfilled sensing Hg 2+ in environmental water with satisfactory recoveries (90.3%−107.8%) and relative standard deviation (RSD) of less than 3.81%, manifested their excellent environmental tolerance. Notably, the O-CDs based test paper was fabricated and served as a portable sensor with a smartphone assistant for detection of Hg 2+ , which greatly elevated the sensing performance of test paper. This work contributed an innovative approach for the synthesis of O-CDs, as well as a reliable sensitive sensor based on portable test paper for an intelligent readable recognizing system, which could be regarded as promising candidate for real detection of Hg 2+ .
研究领域:
环境科学
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分析化学
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纳米材料科学
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产品关联
DOI
高影响因子
Nanoscale
·
2015
·Vol. 7
·期 33
·13877-13887
·
IF 7.394
Xiaoqian Ge,Lining Sun,Binbin Ma,Di Jin,Liang Dong,Liyi Shi,Nan Li,Haige Chen,Wei Huang
We have constructed a multifunctional nanoprobe with sensing and imaging properties by using hollow mesoporous silica coated upconversion nanoparticles (UCNPs) and Hg2+ responsive ruthenium (Ru) complex. The Ru complex was loaded into the hollow mesoporous silica and the UCNPs acted as an energy donor, transferring luminescence energy to the Ru complex. Furthermore, polyethylenimine (PEI) was assembled on the surface of mesoporous silica to achieve better hydrophilic and bio-compatibility. Upon addition of Hg2+, a blue shift of the absorption peak of the Ru complex is observed and the energy transfer process between the UCNPs and the Ru complex was blocked, resulting in an increase of the green emission intensity of the UCNPs. The un-changed 801 nm emission of the nanoprobe was used as an internal standard reference and the detection limit of Hg2+ was determined to be 0.16 μM for this nanoprobe in aqueous solution. In addition, based on the low cytotoxicity as studied by CCK-8 assay, the nanoprobe was successfully applied for cell imaging and small animal imaging. Furthermore, when doped with Gd3+ ions, the nanoprobe was successfully applied to in vivo magnetic resonance imaging (MRI) of Kunming mice, which demonstrates its potential as a MRI positive-contrast agent. Therefore, the method and results may provide more exciting opportunities to afford nanoprobes with multimodal bioimaging and multifunctional applications.
研究领域:
材料科学
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生物医学工程
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分析化学
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产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2019
·
IF 6.393
Fuyou Du,Lingshun Sun,Qiulian Zen,Wei Tan,Zhenfang Cheng,Guihua Ruan,Jianping Li
A simple and rapid fluorescence “on-off-on” approach for sensing Hg 2+ and paraquat was designed by using nitrogen doped graphene quantum dots (N-GQDs) and N-GQDs/Hg 2+ system, respectively. It was found that the fluorescence of N-GQDs solution was quenched in the presence of Hg 2+ ions (turn off), and the quenched N-GQDs/Hg 2+ system was recovered after addition of paraquat (turn on), due to the higher affinity of paraquat and Hg 2+ than that of N-GQDs and Hg 2+ that resulting into the release of Hg 2+ from the surface of N-GQDs and the fluorescence recovery of N-GQDs. The developed fluorescence “on-off-on” approach exhibited high selectivity and sensitivity for detection of Hg 2+ and paraquat. Under the optimum experimental conditions, good linearities were achieved over the range of 0–4.31 μM for Hg 2+ and 0.05–2.0 μg mL −1 for paraquat, respectively. The detection limits for Hg 2+ and paraquat were 23 nM and 19 μg L −1 , respectively, and the recoveries of Hg 2+ and paraquat spiked in real samples ranged from 95% to 105.8%. Results of this study suggested that the highly selective and sensitive fluorescence “on-off-on” approach can be used to the detection of Hg 2+ and paraquat in real environmental water samples.
研究领域:
环境科学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
Journal of Environmental Chemical Engineering
·
2024
·
IF 7.4
Wanzhu Wang,Xuhan Ge,Daqing Chen,Lichao Wu,Danhua Ge,Xiaojun Chen
In contrast to peroxidase-mimicking nanozymes, oxidase mimics directly employ molecular oxygen (O 2 ) as oxidant to generate reactive oxygen species (ROS). Generally, transition metal-based nanozymes exhibit poor oxidase-like activity. We herein constructed a colorimetric sensor based on Co-based oxidase mimic, where the Co nanoparticles were encapsulated in hollow N-doped carbon nanospheres (Co-N/C). Co nanoparticles confined catalyst effectively anchored the active part, which greatly influenced the catalytic activity of the oxidase-mimic nanozymes. Due to the antioxidative property of L-Cysteine (L-Cys) and the specific complexation between L-Cys and Hg 2+ , the rationally designed Co-N/C could be utilized to detect L-Cys and Hg 2+ . The proposed sensing platform exhibited wide linear ranges of 1–30 μM for L-Cys and 0.6–30 μM for Hg 2+ , respectively. The excellent selectivity of the constructed sensor showed the reliability and practicality for the assay of L-Cys and Hg 2+ in fetal bovine serum. The spatial confinement can prevent the aggregation of Co nanoparticles, boosting the stability and catalytic performance in colorimetric sensing.
研究领域:
生物化学
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纳米医学
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分析化学
✓ 已验证
产品关联
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2020
·
IF 3.594
Li Li,Yun Feng,Yaru Qiu,Yusheng Li,Keyan Wu,Liande Zhu
Selective detection of toxic Hg 2+ is of great significance in the control of the environmental and industrial pollution because it poses serious threat to human health. Herein, we present a differential pulse anodic stripping voltammetric (DPASV) method for Hg 2+ detection by using hollow and porous three-dimensional nano-structured NiCo 2 O 4 with cage-like morphology as an electrode modifier. The NiCo 2 O 4 nanocages (NiCo 2 O 4 NCs) derived from ZIF-67 (a cobaltic zeolite imidazole framework) possess large specific surface areas that can provide a large number of adsorption sites for Hg 2+ . The NiCo 2 O 4 NCs modified glassy carbon electrode (NiCo 2 O 4 NCs/GCE) was demonstrated to be an advantageous and high-performance platform for the electrochemical detection of Hg 2+ with a wide linear range (0.1 nmol/L–3.5 µmol/L) and low detection limit (0.09 nmol/L). Additionally, the NiCo 2 O 4 NCs/GCE was proved to be highly stable, representing it was benign to environment and could be well applied to detect Hg 2+ in real water samples.
研究领域:
环境科学
·
分析化学
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）