ANALYST
·
2019
·Vol. 144
·期 8
·2797-2802
·
IF 4.019
Piaopiao Chen,Ke Huang,Rui Dai,Erica Sawyer,Ke Sun,Binwu Ying,Xiawei Wei,Jia Geng
Nowadays, label-free atomic spectrometric bioassays are attracting great research interest because of their advantages of low cost, simple design and operation, etc. Herein, a novel and simple chemical vapor generation-atomic fluorescence spectrometry (CVG-AFS)/inductively coupled plasma-mass spectrometry (ICP-MS) label-free detection method is presented for highly sensitive and selective assay of DNA and proteins. This work mainly combined a phenomenon that CdTe quantum dots (QDs) can be used to selectively differentiate free Hg2+ and the T–Hg2+–T complex, with the use of simple membrane filtration separation to improve the performance of the label-free bioassay methods. Upon hybridization with the DNA/protein (carcinoembryonic antigen, CEA) target, the T–Hg2+–T hairpin structure was opened and Hg2+ was released; this initiated the cation exchange reaction between Hg2+ and CdTe QDs which released Cd2+ simultaneously. Subsequently, the free Cd2+ was separated by the filtration membrane without separating the CdTe QDs, which could then be separated from the sample matrices for the CVG-AFS/ICP-MS assay. Under the optimal conditions, this method possessed high sensitivity for DNA and CEA determination with limits of detection (LODs) of 0.2 nM and 0.2 ng mL−1, and linear dynamic ranges of 1–160 nM and 0.5–20 ng mL−1, respectively, and exhibited excellent DNA sequence specificity and protein selectivity. This method preserves the advantages of the label-free atomic spectrometric bioassay, and combined with the selective cation exchange reaction and simple filtration separation to improve the performance.
研究领域:
分析化学
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生物医学工程
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分子生物学
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DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2019
·
IF 6.35
Lei Li,Qiang Wang,Yuan Yang,Li Luo,Ru Ding,Zhao-Guang Yang,Hai-Pu Li
For understanding the environmental behavior and toxicity of Ag nanoparticles (Ag-NPs), a quantitative method for characterizing the AgNPs in soils and sediments is urgently needed. In this study, we validated a previously developed extraction method by optimizing the extraction of silver nanoparticles from soil and sediment samples to which engineered AgNPs had been added. The samples were analyzed by single particle inductively coupled plasma mass spectrometry (SP-ICP-MS). Initially, different extraction conditions were evaluated to validate the optimal extraction procedure. Then the optimal extraction procedure was applied to environmental soil and sediment samples spiked with AgNPs. The extraction data for Ag-NPs with sizes below 30 nm was not shown due to the particle size detection limit of the SP-ICP-MS method (∼20 nm). The number concentrations of Ag particles extracted from different environmental soils and sediments matrices were in the range of (12 ± 1–23 ± 1) × 107 particles/g soil. Similarly, 53.4–100.0% of the particulate Ag mass was recovered. For the relatively low Ag mass recovery of Guiyu agricultural soil, the Ag mass recovery shows great improvement (from 53.4 to 105.8%) by the soil dilution using SiO2. The optimal method was validated to be feasible for extracting Ag-NPs from environmental soils and sediments, except for the soil with high soil organic matter (SOM) content. The SiO2 dilution of soil provides a promising way to promote the extraction of Ag-NPs in soil (or sediment) with high SOM content, which could further promote the study on the environmental behavior and toxicity of Ag-NPs in soil and sediment environment.
研究领域:
环境科学
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分析化学
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纳米技术
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土壤学
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PubMed
高影响因子
MICROCHIMICA ACTA
·
2018
·Vol. 185
·期 9
·1-8
·
IF 5.705
Cheng Heyong,Zhang Wenwen,Wang Yuanchao,Liu Jinhua
This work demonstrates the power of graphene and graphene oxide (GO) as stationary phases for high performance liquid chromatography (HPLC) based elemental speciation analysis combined with detection by inductively coupled plasma mass spectrometry (ICP-MS). Inorganic mercuric and organomercuric compounds coordinate with 2-thiosalicylic acid (TSA) to form Hg-TSA complexes. These complexes are retained by GO owing to its strong π electron stacking capability for TSA. Separation of the four mercury species tested was achieved within 12 min with resolutions of 1.8–3.4. Similarly, inorganic anionic species of arsenic and selenium, and organoarsenicals are electrostatically attracted by aromatic quaternary ammonium cations in the mobile phases. Organoarsenicals also can be separated by using long alkyl quaternary ammonium compounds. Aromatic quaternary ammonium compounds possess particularly high affinity to GO because of strong π interaction. This leads to effective retention of the As/Se anions. A comparison between graphene and GO as stationary phases for HPLC separation of mercury and arsenic species demonstrates negligible difference. Arsenic species are separated within 32 min, and selenium species are achieved within 20 min. The mobile phase also allows efficient separation of iodate, iodide, bromate, bromide, chromic acid and chromate. Analysis of a certified fish tissue by HPLC-ICP-MS using the GO@SiO 2 column demonstrates its feasibility for routine elemental analysis. Good agreement is found between experimental results and certified values, with recoveries ranging between 92 and 96%.
Graphene oxide as a stationary phase for high performance liquid chromatography with inductively coupled plasma mass spectrometric detection (HPLC-ICP-MS) in speciation analyses of mercury, arsenic, selenium, iodine, bromine and chromium is achieved for the first time.
研究领域:
分析化学
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环境科学
·
材料科学
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DOI
PubMed
高影响因子
CHEMICAL GEOLOGY
·
2024
·
IF 3.6
Xinting Lü,Detao He,Yongsheng Liu,Ming Li,Jie Lin,Wei Chen,Lüyun Zhu,Ao Yang,Yantong Feng,Zhenyi Liu,Xianli Zeng,Zhaochu Hu
Iron oxide minerals, such as hematite and magnetite, are commonly found in a wide range of geological environments. Their Fe isotopic composition acts as a potent geochemical tracer, finding applications across various realms of Earth sciences. However, natural iron oxide minerals often have a high Cr content up to percentile levels, necessitating correction of isobaric interference caused by 54 Cr + on 54 Fe + to ensure accurate and precise in situ Fe isotopic compositions. In this study, a pure chromium metal with homogeneous isotopic compositions was examined within the analysis sequence to obtain the fractionation factor of Cr (β Cr ) for isobaric interference correction. A femtosecond laser ablation system combined with wet plasma conditions was used to decrease the matrix effect. We synthesized a series of magnetite and hematite samples with simple matrix as well as natural magnetite samples with complex matrix to evaluate the feasibility of Cr interference correction. By employing our proposed correction method, the deviation caused by the isobaric interference of 54 Cr + was effectively eliminated. The corrected δ 56 Fe values for hematite and magnetite samples, even with a Cr/Fe ratio of as high as 1.27, exhibited good agreement with the reference values within the long-term reproducibility uncertainty of 0.10‰. These results indicate the robustness of Cr interference correction in obtaining accurate Fe isotopic compositions of Cr-rich iron oxides.
研究领域:
地球化学
·
地质学
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DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2022
·
IF 9.221
Jiawei Shen,Tingting Li,Min Wang,Bo Yao
MicroRNAs (miRNAs) are a category of short non-coding RNA molecules which play essential roles in regulating gene expression. Correspondingly, the aberrant expression of miRNAs is commonly related to the development of various diseases including cancers and cardiovascular diseases. Hence, miRNA is considered as an ideal biomarker for disease diagnosis and its quantification is highly demanded. Herein, an isothermal and enzyme-free miRNA assay based on catalytic hairpin assembly (CHA) amplification coupled with inductively coupled plasma mass spectrometry (ICP-MS) detection is proposed. Two hairpin probes are employed in CHA reaction with rare earth element tag on H1 for ICP-MS detection and biotin on H2 for immobilization on magnetic beads. In the presence of target miRNA, the two independent probes open up their stem-loop structure and hybridize. The target miRNA will be released after the hybridization to trigger another CHA reaction. Ultimately, one target miRNA molecule promotes a number of CHA cycles and produces several copies of hybridized complex leading to the enhanced ICP-MS signal. Based on this approach, miRNA-141 was successfully analyzed with high sensitivity and specificity. The limit of detection (LOD) was 88 fmol with a linear range 0.1–2 pmol. Compared with fluorescence mediated CHA method and ICP-MS mediated basic sandwich method, the current strategy displayed the lowest LOD. The developed method was also applied to analyze miRNA-141 and miRNA-21 simultaneously to demonstrate its multiple detecting capability and practical applicability. Furthermore, this miRNA assay has the potential to become a high-throughput method by employing more distinct rare earth elements.
研究领域:
分子生物学
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分析化学
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生物医学工程
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癌症研究
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心血管疾病
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DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2026
·
IF 5.2
Junyi Wu,Yao Liu,Dongwei Zhu,Jianxing Xu,Jian Yun
A DSN-triggered Y-shaped dual-circular-probe DNA walker is developed for RCI-associated miR-29a detection. • The Y-shaped steric hindrance structure effectively suppresses non-specific DSN cleavage, ensuring ultralow background. • The cascade amplification strategy achieves a detection limit as low as 0.26 fM for miR-29a. • The ICP-MS-based Rh readout provides label-free, photobleaching-free, and robust quantification. • The method exhibits excellent single-base mismatch discrimination and reliable performance in serum and cell samples. Rotator cuff injury (RCI) is a prevalent musculoskeletal disorder with limited early diagnostic options, partly due to the lack of sensitive and non-invasive biomarkers. Magnetic resonance imaging (MRI), the current mainstream imaging tool for RCI, suffers from high cost, limited accessibility, and an inability to reflect molecular-level pathological changes before structural damage becomes evident. MicroRNAs (miRNAs) have shown promise as diagnostic indicators, yet their reliable detection in clinical contexts requires methods with ultrahigh sensitivity and single-base resolution. Here, we report a duplex-specific nuclease (DSN)-triggered DNA walker with dual circular probes for highly specific and ultrasensitive analysis of RCI-associated miR-29a by inductively coupled plasma mass spectrometry (ICP-MS). The DNA walker is assembled on magnetic beads, where the walking track comprises two circular probes (c1 and c2) forming a Y-shaped steric hindrance structure that effectively suppresses non-specific DSN cleavage, ensuring an ultralow background. Upon target miR-29a hybridization, DSN specifically cleaves the DNA–RNA hybrid, initiating a cascade amplification involving self-priming, nicking/polymerase cycling, rolling circle amplification, and DNAzyme-mediated cleavage of a rhodium-labeled substrate. The released rhodium is quantified by ICP-MS, providing a photobleaching-free readout. The proposed method achieves a detection limit as low as 0.26 fM with outstanding single-base mismatch discrimination, a wide dynamic range from 1 fM to 100 pM, excellent recoveries in serum (93.2–103.2%), and high correlation with RT-PCR (R 2 = 0.9912) in real cell line samples. This DSN-triggered DNA walker offers a robust, sensitive, and specific platform for RCI-related miRNA detection, holding great promise for early diagnosis and prognosis monitoring of rotator cuff injuries. Download: Download high-res image (78KB) Download: Download full-size image
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高影响因子
CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
·
2025
·
IF 1.3
Kewei Wang,Xilong Qian,Ying Zhang,Zheng Liu,Weihua Zhang,Liu Zhou,Yanqiong Pan,Liwen Zheng,Fang Fang,Yang Zhang,Yehuang Wang,Bin Jiang,Shengjin Liu
Yuhuang Shengji ointment (YSO) is a traditional Chinese medicine clinical prescription from Professor Wang Yehuang, a famous Chinese medicine professor in Jiangsu Province China.It is used for the treatment of postoperative healing of anal fistula in clinic practice. It is composed of Corydalis Rhizoma, Radix Sanguisorbae Carbonisata, Natrii Sulfas, Alumen Ustum, Plant Soot and Borneolum. It has the effects of eliminating dampness, astringing sores, hemostasis, pain relief, detumescence and muscle regeneration. According to the characteristics that YSO mainly contains inorganic, organic and volatile components, the comprehensive quality control of the ointment was achieved by different analytical methods. The organic components in the ointment were qualitatively analyzed by UPLC-Q-TOF-MS to determine the main chemical components. HPLC fingerprint of the organic components of the ointment was further established. ICP-MS technology is used to analyze the inorganic elements in ointment, which provides data support for the quality control of inorganic components. The Flash GC e-nose is used for rapid detection of volatile substances in ointment, and the fingerprint of volatile components is established. The comprehensive use of these analytical techniques not only reflects the overall chemical characteristics of the ointment, but also helps to evaluate the consistency between different batches of products. This study successfully established a comprehensive quality analysis method, which provided a basis for the quality control of YSO. At the same time, it provides a more comprehensive quality control strategy for ointment which mainly contains organic, inorganic and volatile components.
研究领域:
中药学
·
分析化学
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DOI
高影响因子
TALANTA
·
2016
·
IF 4.035
Yuan Yang,Li Luo,Hai-Pu Li,Qiang Wang,Zhao-Guang Yang,Chen-Lu Long
In this study, cloud point extraction (CPE) combined inductively coupled plasma mass spectrometry (ICP-MS) was used to determinate silver nanoparticles (AgNPs) in environmental water. AgNPs dispersed in water could be extracted into surfactant phase based on optimized CPE parameters which including pH, incubation temperature and equilibration time, the content of nonionic surfactants (Triton X-114, TX-114) and Na 2 S 3 O 3 . Good separation results of AgNPs and ionic silver Ag(I) in aqueous environment was obtained with the addition of Na 2 S 3 O 3 when the concentration of Ag(I) was lower than ten times of AgNPs. High recoveries of different diameter AgNPs spiked into ultrapure water were obtained. The influence of environmentally relevant matrix on CPE of AgNPs was studied in detail. Our results showed that there were little interference of environmentally relevant ions and five representative engineering nanomaterials (ENMs) on CPE of AgNPs. Moreover high recoveries and good separation were obtained when natural organic matter (NOM) up to 30 mg/L mixed with AgNPs in ultrapure water. The detection limit of AgNPs in water using CPE combined ICPMS was 5 ng/L. The recoveries of the AgNPs spiked into natural and effluents water with this method were in the range of 79±1–123±13%. Furthermore CPE-ICPMS and Single particle-ICPMS were used to study the UV induced photochemical transformations of AgNPs suspension at environmental relevant concentrations.
研究领域:
环境科学
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分析化学
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DOI
PubMed
高影响因子
GEOSTANDARDS AND GEOANALYTICAL RESEARCH
·
2013
·Vol. 38
·期 1
·37-50
·
IF 2.77
Jie Li,Xiao-Ying Jiang,Ji-Feng Xu,Li-Feng Zhong,Xuan-Ce Wang,Gui-Qin Wang,Pei-Pei Zhao
en This link goes to a English section fr This link goes to a French section We report an improved procedure for the determination of the platinum-group elements (PGE) and Re, and Os isotopes from a single sample aliquot by isotope dilution (ID) using inductively coupled plasma-mass spectrometry (ICP-MS) and negative thermal ionisation mass spectrometry (N-TIMS), respectively. A two-stage column method was used to purify PGE-Re from their sample matrix and interfering elements (e.g., Mo, Zr and Hf) after Os had been separated by CCl 4 solvent extraction. The first column separation step used cation exchange resin (AG50W-X8) to concentrate PGE-Re and some potential interfering elements (e.g., Mo, Zr and Hf). In the second step, N -benzoyl- N -phenylhydroxylamine (BPHA) extraction resin was used to separate PGE-Re from the remaining interfering elements, which all remained strongly absorbed to the resin. The method was used to determine the PGE and rhenium, and Os isotope ratios in a range of geochemical reference materials (TDB-1, WGB-1, BHVO-2 and UB-N). The obtained results agree well with those previously published. This new method enables PGE-Re abundances and Os isotopic ratios to be determined on the same sample digestion, and circumvents the problems created by sample heterogeneity when comparing PGE and Re-Os isotope data. en This link goes to a English section fr This link goes to a French section Nous décrivons une procédure améliorée pour la détermination des abondances des éléments du groupe du platine (EGP), du Re et des isotopes de l'Os à partir d'un même aliquot d'échantillon par dilution isotopique (ID) en utilisant respectivement la spectrométrie de masse à source plasma (ICP-MS) et la spectrométrie de masse par ionisation thermique négative (N-TIMS). Une méthode sur colonne en deux étapes a été utilisé pour purifier les EGP et le Re de la matrice de l'échantillon et des éléments interférents (par exemple, Mo, Zr et Hf) et ceci après que l'Os ait été séparé par extraction par solvant CCl 4 . La première étape de séparation sur colonne utilise une résine échangeuse de cations (AG50W-X8) pour concentrer les EGP et le Re et certains éléments interférents potentiels (par exemple, Mo, Zr et Hf). Au cours de la seconde étape, la résine d'extraction N-benzoyl-N-phénylhydroxylamine (BPHA) a été utilisée pour séparer les EGP et le Re des éléments interférents restants, qui sont tous fortement absorbé par cette résine. La méthode a été utilisée pour déterminer les concentrations en PGE et Re, et les rapports isotopiques de l'Os dans une gamme de matériaux de référence géochimiques (TDB-1, WGB-1, BHVO-2 et UB-N). Les résultats obtenus sont en bon accord avec ceux publiés antérieurement. Cette nouvelle méthode permet la détermination des abondances en PGE et Re et des rapports isotopiques de l'Os sur la même portion d'échantillon digérée, et contourne ainsi les problèmes liés à l'hétérogénéité des échantillons lorsqu'on compare les données élémentaires des EGP et les données isotopiques Re-Os.
研究领域:
地球化学
·
分析化学
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DOI
高影响因子
Toxics
·
2024
·Vol. 12
·期 5
·325
·
IF 3.9
Ge Song,Honglian Tan,Chuhan Cheng,Peng Li,Xinyang Sun,Yuling Zhou,Yong Fang
Hexavalent chromium (Cr(VI)) is known as the most hazardous species of chromium. Speciation analysis of Cr in foods is of a great significance for assessing its influences on human health. In this study, a fast HPLC-ICP-MS method for the determination of Cr(VI) was developed for determining the content of Cr(VI) and also investigating its transformation in foods. The developed method employs an alkali extraction and weak anion-exchange column separation for distinguishing the Cr species, facilitating accurate Cr(VI) quantification within 1.5 min. This technique was applied to determine the Cr(VI) levels in a range of food products, including yoghurt, milk powder, rice flour, orange juice, green tea, white vinegar, and whole wheat bread. The results showed that no Cr(VI) was detected in these food products. Spiking experiments revealed that the recovery rate of Cr(VI) decreased with the increase in its contact time with food products. A further exploration of Cr(VI) in various food components such as vitamin C, tea polyphenols, whey proteins, gelatin, fructose, and cellulose indicated the conversion of Cr(VI) to organic Cr(III) over a period from 20 min to 60 h. It was found that high temperatures and acidic conditions accelerated the rate of Cr(VI) conversion to organic Cr(III) in the six food components mentioned above. This evidence suggests that natural reducing substances in foods probably prevent the occurrence of Cr(VI).
研究领域:
分析化学
·
食品科学
·
环境健康
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PubMed
高影响因子
JOURNAL OF DISPERSION SCIENCE AND TECHNOLOGY
·
2020
·
IF 1.701
Long Wang,Zhiming Wang,Cuo Zhou,Weijun Song,Chunyan Sun
A cesium potentiometric microsensor based on magnetic ion-imprinted polymer (MIIP) has been developed for Cs(I) sensing. The core-shell structure Cs(I)-MIIP was synthesized by surface-imprinting technique, using functionalized magnetic Fe3O4@SiO2 microspheres, Cs(I) ion and carboxymethyl chitosan as supporter, template and functional monomers respectively. The resultant Cs(I)-MIIP was used as ionophore in the construction of the PVC membrane electrode to obtain a selective potentiometric microsensor toward Cs(I) ions. The fabricated sensor exhibited a highly sensitive Nernstian response (60.2 mV/decade) over the concentration range of 1 × 10−6 to 1 × 10−1 M of Cs(I). The response time of the microsensor was considerably short (less than 3 s) and its detection limit was determined as 3.0 × 10−7 M. The microsensor was effectively performed in the pH range of 4–6, and revealed desirable selectivity over a wide variety of other cations including alkali, alkaline earth and heavy metals. The proposed microsensor was also used for the determination of Cs(I) contents in the environmental water samples. The potentiometric results were in good harmony with the results obtained by the ICP-MS method.
研究领域:
分析化学
·
环境科学
·
材料科学
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DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2024
·
IF 6.7
Wenxiao Tang,Beibei Chen,Man He,Gaofei Song,Yonghong Bi,Bin Hu
Understanding the interactions between mercury and microalgae, especially the interactions between inorganic mercury (IHg) and extracellular polymeric substances (EPS, a protective barrier between cells and their external environment), is essential for elucidating mercury’s toxicological mechanisms. Given the inherent cell heterogeneity, a novel analysis system of an online viscoelastic fluid focusing chip-time-resolved analysis inductively coupled plasma mass spectrometry has been developed to investigate the bioaccumulation of HgS nanoparticles and Hg2+ in single Microcystis aeruginosa (M. aeruginosa) cells, exploring the interaction mechanisms between HgS/Hg2+ accumulation in algal cells and EPS. The single-cell analysis results reveal minimal bioavailability of HgS within algal cells, with mercury’s toxicity to M. aeruginosa being species-dependent. Notably, algal cells exhibited more heterogeneity in HgS uptake than in Hg2+ uptake. Under Hg2+/HgS stress, M. aeruginosa cells with EPS removed (EPS-R algal cells) showed an increased level of bioaccumulation of mercury compared to those with EPS (EPS-C algal cells), highlighting the critical role of EPS in mercury bioaccumulation. Overall, the designed viscoelastic fluid microfluidic focusing chip integrates focusing and cleaning functions, featuring easy fabrication, simple operation, low sample loss, and relatively high throughput. Under the optimal conditions, the sample throughput is 1195 min–1 and the cell recovery is 90%. Besides, this research offers novel insights into the interaction mechanisms between Hg2+/HgS and EPS in microalgal cells and unveils the specific toxic effects of Hg2+/HgS on M. aeruginosa at the single-cell level, contributing to a deeper understanding of mercury’s ecological and toxicological impact in aquatic environments.
研究领域:
环境科学
·
毒理学
·
分析化学
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PubMed
高影响因子
危险品化学品经营许可证（带存储）