Frontiers in Chemistry
·
2023
·
IF 5.5
Zisan Zeng,Yang Zhao,Luoxing Yang,Fengna Xi,Danke Su
Designing fast and simple quantitative methods on cheap and disposable electrodes for the early detection of HeLa cells is highly desirable for clinical diagnostics and public health. In this work, we developed a label-free and sensitive electrochemical cytosensor for HeLa cell detection based on the gated molecular transport across vertically ordered mesoporous silica films (VMSFs) on the disposable indium tin oxide (ITO) electrode. As high affinity for a folate receptor existed on the membrane of HeLa cancer cells, folic acid (FA) functionalized VMSF could regulate the transport of electrochemical probe (Fe(CN) 6 3− ) by the specific recognition and adhesion of HeLa cells toward the VMSF surface. In addition, VMSF, served as a solid skeleton, is able to effectively prevent the direct contact of cells with the underlying electrode, remaining the underlying electrode activity and favoring the diffusion of Fe(CN) 6 3− . Once specific adhesion of HeLa cells to the VMSF surface happens, Fe(CN) 6 3− redox probe exhibits impeded transport in the silica nanochannels, ultimately resulting in the decreased electrochemical responses and realizing the quantitative determination of HeLa cells with a broad linear range (10 1 –10 5 cells/mL) and a low limit of detection (4 cells/mL). The proposed electrochemical cytosensor shows a great potential application for the early diagnosis of cervical cancer.
研究领域:
生物医学工程
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分析化学
·
癌症诊断
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
·
2019
·
IF 3.218
Houhuang Zhou,Chenhuinan Wei,Ju Fang,Likang Fu,Gang Liu,Qiming Liu
Poly 3,4-ethylenedioxythiophene (PEDOT) membrane and zirconia nanoparticles (ZrO 2 NPs) were directly prepared on ITO electrode successively through simple strategies. The ZrO 2 NPs-chitosan/PEDOT/ITO electrode was employed as a disposal electrochemical sensor for methyl parathion (MP) detection. The structure characteristics，adsorption characteristics and electrochemical features of electrode were characterized by the SEM, XRD, UV–vis spectrometer and electrochemical analysis techniques. Combined with the individual properties of PEDOT (conductivity and electrocatalysis) and ZrO 2 NPs (affinity to MP), the ZrO 2 NPs-chitosan/PEDOT/ITO electrode displayed superior electrochemical performances to MP measurement with a limit of detection of 2.8 ng·ml −1 (S/N = 3) and a linear range of 5–2000 ng·ml −1 . Furthermore, this sensor exhibited acceptable selectivity and reproducibility. The result of this sensor in real sample measure to MP detection also demonstrates precision.
研究领域:
材料科学
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电化学
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环境监测
·
纳米技术
✓ 已验证
产品关联
DOI
高影响因子
ORGANIC ELECTRONICS
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2020
·
IF 3.31
Guodong Liu,Yu Liu,Meiyun Zhang,Zhenhua Yang,Patrick Gane
Poly(3,4-ethylenedioxythiophene) (PEDOT), a common constituent material used in the preparation of electrochromic devices (ECDs) when doped with the polymer anion poly(styrenesulfonate) (PSS), can also be used to form an electrode layer. Patterned ECDs may thus be fabricated by conventional spin-coating. Due to high wastage and low productivity in spin coating , the demand for low-cost ECDs is difficult to meet. The alternative use of screen printing, however, results in a major challenge due to difficulty in achieving the necessary continuity of the applied print and of the subsequent film forming uniformity when using standard PEDOT:PSS dispersion alone. The film forming property of PEDOT:PSS is shown here to be improved by dispersing in a water methanol mix to form a novel PEDOT:PSS-solvent compound, which, when combined together with the high boiling solvent dimethyl sulfoxide (DMSO) and a thickening binder solution of polyvinyl alcohol (PVOH), allows screen printing to perform well. The sheet conductivity of the printed PEDOT:PSS compound film is then further increased by studying post-treatment using strong and weak organic acids , illustrated by comparing p-toluenesulfonic acid (tosylic acid (PTSA)) with glacial acetic acid (CH 3 COOH (HOAc)) or oxalic acid (OA), respectively, over a range of concentration. In this way, the optimal processing concentration of acid is accordingly obtained to minimize sheet resistance. Screen printed patterned ECDs, based on the post-treated PEDOT:PSS compound electrochromic and electrode films using the optimal acid concentrations, were printed including electrolyte layers, were evaluated. Performance of lateral versus vertical ECD build architecture in respect to chromatic aberration, working voltage, time response, ion mobility in electrolyte type (gel/solution), absorbance at color-switching, as well as mechanical stability during repeated bending, was assessed. The optima of chromatic aberration and absorbance at color-switching were found to coincide with minimal sheet resistance of the novel PEDOT:PSS compound films. Almost equivalent sheet resistance was obtained with both PTSA and HOAc, each at optimal treatment dosage, showing newly that the weak organic acid post-treatment provides an equivalent effect in comparison with strong organic acid if used at sufficient concentration. Furthermore, the electrochromic performance of the experimentally prepared ECDs was shown to be close to the level obtained from conventional indium-tin oxide (ITO)-based ECDs, and retained stable performance for color-switching after repeated bending of 1 000 times.
研究领域:
材料科学
·
电子工程
✓ 已验证
DOI
高影响因子
TALANTA
·
2021
·
IF 6.057
Weiwei Tang,Andrew Gordon,Hui-ying Wang,Ping Li,Jun Chen,Bin Li
The development of in situ methods for the analysis and visualization of enzyme activity is of paramount importance in drug discovery, research, and development. In this work, the functionalized and array patterned indium tin oxide (ITO) glass slides were fabricated by non-covalent immobilization of amphipathic phospholipid-tagged peptides encompassing the thrombin cleavage site on steric acid-modified ITO slides. The fabricated peptide arrays provide 60 spots per slide, and are compatible with matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS) measurement, free matrix peak interference, and tolerance to repeated aqueous washing. The peptide arrays were used for the investigation of thrombin activity and screening for its potential inhibitors. The thrombin activity and its Michaelis-Menten constant (K m ) for immobilized peptide substrate was determined using developed MALDI MS peptide array. To investigate the applicability and effectiveness of peptide arrays, the anti-thrombin activity of grape seed proanthocyanidins with different degrees of polymerization (DP) was monitored and visualized. MALDI MS imaging results showed that the fractions of proanthocyanidins with the mean DP of 4.61–6.82 had good thrombin inhibitory activity and their half-maximal inhibitory concentration (IC 50 ) were below 10 μg/mL. Therefore, the developed peptide array is a reliable platform for the discovery of natural thrombin inhibitors .
研究领域:
药物发现
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分析化学
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药学
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生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Materials Chemistry C
·
2023
·Vol. 11
·期 10
·3634-3643
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IF 6.4
Jundang Liang,Botian Li,Xiaodong Gai,Ning Li,Jian Wang,Yichi Zhang,Qiong Zhou,Yuchao Sun
The excessive use of paper causes serious resource consumption and environmental pollution. Environmentally friendly light printing based on a photochromic system with the advantages of being noncontact, rapid, and inkless has attracted increasing attention; however, several problems need to be addressed, such as slow discoloration and irreversibility. In this work, a composite of MoO3−x inorganic nanorods and the conductive polymer poly(3,4-ethylenedioxythiophene):polystyrene sulfonate (PEDOT:PSS) was employed as a color-changing electrode, and a photochromic/electrochromic strain sensor (PESS) was obtained by assembling the color-changing electrode with an organohydrogel electrolyte and a flexible indium tin oxide (ITO) film. Due to the photochromic/electrochromic effect of MoO3−x and the conductivity of PEDOT:PSS, the sensor turned cyan-blue under UV irradiation or a negative bias voltage, and it could be rapidly bleached by applying a positive bias voltage. The photochromic/electrochromic behavior of the PESS could be optimized by tuning the amount of PEDOT:PSS, and the device could perform cyclic light printing/electroerasing with a fast and stable response. Moreover, the flexible strain sensor could be utilized to monitor mechanical strain by translating a series of deformations into electrical signals. The properties of photochromism/electrochromism and strain sensing make the device potentially applicable to outdoor wearable devices capable of light-based printing.
研究领域:
材料科学
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电化学
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光电
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传感器
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环境科学
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2015
·
IF 5.636
Fei Yan,Yayun He,Longhua Ding,Bin Su
The rapid and sensitive detection of nitroaromatic compounds is of great significance for human health, the environment, and public security. The present work reports on the extraction and electrochemical analysis of trace nitroaromatic compounds, such as explosives and organophosphate pesticides (OPs), using the indium tin oxide (ITO) electrodes modified with a highly ordered and aligned binary assembly of silica mesochannels and micelles (BASMM). With a pore diameter of ca. 2–3 nm, silica mesochannels (SMs) perpendicularly oriented to the ITO electrode surface can provide hard and robust supports to confine the soft cylindrical micelles formed by the aggregation of cationic surfactants, namely, cetyltrimethylammonium bromide (CTAB). Due to the organized self-assembly of hydrocarbon tails of CTAB surfactants, each micelle has a hydrophobic core, which acts as an excellent adsorbent for rapid extraction and preconcentration of trace nitroaromatic compounds from aqueous solutions via the hydrophobic effect. Furthermore, the cylindrical micelles are directly in contact with the underlying electrode surface, to which extracted compounds can freely diffuse and then be reduced therein, thus allowing their determination by means of voltammetry. Using the BASMM/ITO sensor, electrochemical analysis of trace nitroaromatic explosives, including 2,4,6-trinitrotoluene (TNT), 2,4,6-trinitrophenol, 2,6-dinitrotoluene, 3-nitrophenol, and nitrobenzene, and OPs, such as paraoxon, methyl parathion, and fenitrothion, was achieved with a fast response, wide linear range, high sensitivity, and low detection limit at the ppb level. TNT and paraoxon in real apple, tea, and water samples were also determined. By combining the heterogeneous extraction and determination in one ordered binary nanostructure, the BASMM sensor provides a very simple, rapid, and cost-effective way for analysis of nitroaromatic compounds and can be extended to a wide range of lipophilic yet redox-active analytes.
研究领域:
分析化学
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环境科学
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材料科学
·
电化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
TALANTA
·
2017
·
IF 4.162
Xiaofang Ma,Yali Wu,Samrat Devaramani,Caizhong Zhang,Qixia Niu,Mahgoub Ibrahim Shinger,Wenqi Li,Duoliang Shan,Xiaoquan Lu
The GO-COOH/AuNPs/ZnAPTPP nanocomposites were constructed using zinc monoamino porphyrin (ZnAPTPP) through π–π conjugation with carboxylated graphene oxide (GO-COOH) loaded with Au nanoparticles (AuNPs). Prepared materials were characterized by 1 H NMR spectra, UV–vis absorption spectroscopy and electrochemical impedance spectroscopy . ITO electrode surface was modified with the prepared nanocomposites showed a good photocurrent response when the bias potential, −0.1 V was applied. Nanocomposites modified ITO electrode exhibited good photo-response to the 4-nitrophenol (4-NP). ZnAPTPP were excited from HOMO to LUMO under light irradiation , the photoexcited electrons injected into the conduction band of GO-COOH, and then transferred to AuNPs further to the ITO. The presence of GO-COOH and AuNPs improved the separation of photogenerated charges due to their synergetic effect and excellent conductivity. Externally added 4-NP scavenges the photogenerated holes i.e. it acts as a sacrificial electron donor thereby it enhances the photocurrent of the system. Based on this interaction, a novel method for photoelectrochemical detection of 4-NP was developed with a linear range from 0.1 to 15 nmol/L (r = 0.996) and detection limit of 0.04 nmol/L (S/N = 3). Proposed method is simple and sensitive and this was successfully applied for the quantification 4-NP in river water sample matrices.
研究领域:
材料科学
·
分析化学
·
光电化学
·
环境监测
✓ 已验证
产品关联
DOI
PubMed
高影响因子
EUROPEAN POLYMER JOURNAL
·
2020
·
IF 3.862
Minchao Gu,Zhizheng Zhao,Jiaxuan Liu,Gang Liu,Bin Zhang,Mohamed E. El-Khouly,Yu Chen
Strong demand has been raised for the lightweight, wearable and flexible electronic devices such as wearable computers, paper-like E-readers and epidermal electronics. By using MWNTs-PhBr as a key one dimensional template, novel MWNTs (multi-walled carbon nanotubes) covalently functionalized with poly[(1,4-diethynyl- benzene)- alt -9,9-bis-(4-diphenylaminophenyl)fluorene] (PDDF- g -MWNTs), in which the weight percentage of MWNTs is about 22.74, were successfully synthesized. The as-prepared flexible electronic device with a configuration of Al/PDDF- g -MWNTs/ITO-coated polydimethylsiloxane shows typical nonvolatile rewritable memory performance, with a higher ON/OFF current ratio of more than 10 4 , a switch-on voltage of +2.07 V and a switch-off voltage of −2.45 V. With increasing the film thicknesses, the ON/OFF current ratio of the device was changed from 1 × 10 4 (80 nm) to 2 × 10 4 (100 nm) to 4 × 10 4 (130 nm). Both the switch-on and switch-off voltages almost kept unchanged even if the film thickness was increased from 80 to 130 nm. Upon bending and stretching, the corresponding electronic device still shows good stability and reliability. Electrons dominate the conduct process in PDDF- g -MWNTs during the operation of the device.
研究领域:
材料科学
·
纳米技术
·
电子学
·
柔性电子
·
存储器器件
✓ 已验证
DOI
高影响因子
SOLAR ENERGY MATERIALS AND SOLAR CELLS
·
2016
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IF 4.732
Xuan Liu,Xinxin Xia,Qiuquan Cai,Feilong Cai,Liyan Yang,Yu Yan,Tao Wang
We report efficient, planar heterojunction perovskite solar cells prepared via bar coating that are compatible with large-scale fabrication on flexible substrates . Our MAPbI 3 perovskite devices by bar coating method achieved a maximum power conversion efficiency (PCE) of 13.0% using a ITO/PEDOT: PSS/CH 3 NH 3 PbI 3 (MAPbI 3) /PCBM/Ag structure, and there is very weak hysteresis in these devices. After formulation of the precursor solution and optimization of the coating process , planar perovskite thin films containing large-size grains with low surface roughness were obtained. We found that anti-solvent plays an important role in determining the uniformity, size of grains and width of grain boundaries in perovskite films. The addition of 5 vol% isopropanol into the precursor solution leads to the formation of large aspect ratio grains with a length ca. 200 µm and width of a few hundred nanometers , together with narrow grain boundaries. However, the absence or excessive of isopropanol result in perovskite films with high surface roughness and broad grain boundaries due to inefficient crystal growth or the formation of ridges between crystal grains. These grain boundaries have high PL intensity due to charge recombination and consequently deteriorate device efficiency. When bar-coating the perovskite films in air, best devices with PCE over 11% can be achieved with a relatively high humidity around 50%, eliminating the low humidity requirement during air processing.
研究领域:
材料科学
·
太阳能
·
纳米技术
✓ 已验证
DOI
高影响因子
Journal of Materials Chemistry C
·
2023
·Vol. 11
·期 25
·8438-8445
·
IF 6.4
Yuting Sun,Ziyan Liu,Yuanlin Li,Tianfu Xiang,Aijun Li,Yuhong He,Haotong Wei,Shin-ichi Sasaki,Hitoshi Tamiaki,Xiao-Feng Wang
We have successfully prepared a bio-photodetector based on chlorophyll derivatives as all photosensitive materials. Zinc methyl 3-devinyl-3-hydroxymethyl-pyropheophorbide-a (Chl-A) and methyl 131-deoxo-131-dicyanomethylene-pyropheophorbide-a (Chl-D) were used as electron acceptor and donor, respectively, where the two formed a heterojunction. Although the energy alignment of the photoactive layers of the device studied here is different from that of traditional photodetectors, the device can work well in a self-driven mode. The device has an optimized configuration, i.e., indium tin oxide (ITO)/ZnO/Chl-A/Chl-D/MoO3/Ag, and exhibited excellent performance at self-biasing. When ZnO was applied as the electron transport layer in the ITO/ZnO/Chl-A/Chl-D/MoO3/Ag bio-photodetector device, the dark current density reduced significantly. Besides, the responsivity of the device reached 92.66 mA W−1, which is an increase by ~34 times compared to the ITO/Chl-A/Chl-D/MoO3/Ag device, and the specific detectivity reached 2.48 × 1011 Jones.
研究领域:
材料科学
·
光电技术
·
生物工程
·
光化学
✓ 已验证
产品关联
DOI
高影响因子
Analytical Methods
·
2015
·Vol. 7
·期 5
·1745-1750
·
IF 1.821
Guolin Yuan,Junlin He,Yuan Li,Wailan Xu,Liuliu Gao,Chao Yu
In this work, a novel, ultrasensitive electrochemical immunosensor has been fabricated for the determination of gypican-3 (GPC3) in human serum. A disposable indium tin oxide (ITO) glass array was used as the working electrode on which pyrrole-α-carboxylic acid (Py-α-COOH) was electropolymerised. This approach provided a high content of surface-confined carboxyl groups suitable for the direct covalent binding of GPC3 antibody. In the immunosensor system, the “fluorescence microscopy” technique was first used for the characterisation of carboxyl groups. Meanwhile, the electrode was characterised by scanning electron microscopy (SEM) and electrochemical impedance spectroscopy (EIS). Using GPC3 as a model analyte, the immunoassay exhibited high sensitivity, acceptable stability and reproducibility with a wide linear range from 0.9 pg mL−1 to 9 ng mL−1 and an ultra-low detection limit of 0.3 pg mL−1 (S/N = 3). The results for real sample analysis in human serum demonstrated that the newly constructed immunosensor array provided a rapid, simple and cost-efficient immunoassay with high throughput and sufficiently low detection limits for clinical applications.
研究领域:
分析化学
·
生物传感器
·
电化学
·
医学诊断
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2024
·
IF 6.7
Miao Zhang,Haowei Zhang,Yunlei Zhou,Huanshun Yin,Zhengkun Yu,Xinyue Zhang,Shiyun Ai,Minghui Wang
The OPECT biosensing platform, which connects optoelectronics and biological systems, offers significant amplification and more possibilities for research in biological applications. In this work, a homogeneous organic photoelectrochemical transistor (OPECT) biosensor based on a Bi2S3/Bi2MoO6 heterojunction was constructed to detect METTL3/METTL14 protein activity. The METTL3/METTL14 complex enzyme was used to catalyze adenine (A) on an RNA strand to m6A, protecting m6A-RNA from being cleaved by an E. coli toxin (MazF). Alkaline phosphatase (ALP) catalyzed the conversion of Na3SPO3 to H2S through an enzymatic reaction. Due to the adoption of the strategy of no fixation on the electrode, the generated H2S was easy to diffuse to the surface of the ITO electrode. The Bi2S3/Bi2MoO6 heterojunction was formed in situ through a chemical replacement reaction with Bi2MoO6, improving photoelectric conversion efficiency and realizing signal amplification. Based on this “signal on” mode, METTL3/METTL14 exhibited a wide linear range (0.00001–25 ng/μL) between protein concentration and photocurrent intensity with a limit of detection (LOD) of 7.8 fg/μL under optimal experimental conditions. The applicability of the developed method was evaluated by investigating the effect of four plasticizers on the activity of the METTL3/METTL14 protein, and the molecular modeling technique was employed to investigate the interaction between plasticizers and the protein.
研究领域:
生物化学
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生物传感器
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光电化学
·
蛋白质活性检测
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）