ANALYTICAL CHEMISTRY
·
2022
·
IF 8.008
Mingyue Zhao,Xin Liu,Yuzhu Hou,Tongtong Yang,Jiaquan Xu,Rui Su
The levels of l-arginine and asymmetric dimethylarginine (ADMA) and the amount of the nitric oxide (NO) production have recently been linked to breast cancer and pharmaceutical effect evaluation. Herein, a method combining electrochemistry and high-resolution mass spectrometry (HRMS) was established and used to study NO metabolism and its modulation by ginsenoside compound K (CK) in breast cancer cells. Platinum nanoparticles-decorated fluorine tin oxide was employed as an electrochemical sensor for in situ detection of NO release, while HRMS was used for the analysis of the NO-related metabolites. Through the combination of the electrochemical and HRMS results, decreases in arginine and NO and increases in ADMA and ornithine were observed after modulation by CK, and two highly correlated metabolic pathways including arginine and proline metabolism and vascular smooth muscle contraction were found. This method offers a new strategy for fast evaluation of pharmaceutical efficacy based on NO metabolism.
研究领域:
分析化学
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生物化学
·
药理学
·
癌症研究
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FUEL
·
2023
·
IF 7.4
Yang Du,Shi-Qi Yang,Yun-Jie Li,Zhi-Bin Wang,Xing-Bao Wang,Zhen-Yi Du,Wen-Ying Li
Nickel-based catalysts have been broadly studied for the steam reforming of coal/biomass tar due to their inexpensiveness and good activity. Nevertheless, their industrial application has long been restricted by the deactivation induced by carbon deposition and Ni sintering in the reforming process. Herein, ordered mesoporous nickel-alumina catalysts with varying K contents were prepared. Their performance in steam reforming of toluene as a model compound of tar was evaluated to reveal the effects of K doping. Among them, the catalyst with 2 wt% K loading displayed the best catalytic activity and stability. Experimental and theoretical calculation results suggested that K doping enhances the water adsorption capability of the catalysts, which thereby reduces the amount and graphitization degree of carbon deposits. Moreover, the K doping reduces the acid amount of the support and thereby inhibits the formation of carbon deposits as well. However, K doping does not affect the reaction pathway of the catalysts in toluene steam reforming, and the main reaction intermediates detected with in-situ DRIFTS remain the carbonate groups. Furthermore, the confinement effects of the ordered mesoporous structures inhibit the sintering of Ni particles and the formation of carbon deposits. In addition, the influence of different spatial positions of K on the catalysts was explored. It was found that the K introduction via the one-pot solvent evaporation induced self-assembly method can suppress the loss of K from the catalysts.
研究领域:
催化
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化学工程
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材料科学
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产品关联
DOI
高影响因子
ELECTROCHIMICA ACTA
·
2021
·
IF 6.901
Ting Liu,Li Li,Tianhao Yao,Yanni Li,Lei Zhu,Fang Li,Xiaogang Han,Yonghong Cheng,Hongkang Wang
To explore advanced anode materials with superior Li + /Na + /K + storage performances for alkaline ion batteries (AIBs), a hybrid of amorphous vanadium oxide uniformly embedded into the highly defective carbon nanofibers (denoted as VO x @CNFs) was fabricated via a facile electrospinning method followed by proper heat treatment in this work. The integrated structure synergistically combines the advantages of the amorphous VO x and the defective carbon matrices, which can not only bring abundant active sites for ion storage and multi-channels for ion diffusion, but also well accommodate the structural change induced electrode pulverization . When evaluated as anode materials for AIBs, the optimized VO x @CNFs demonstrate excellent alkaline ion storage performances with high-specific capacities, long-cycling life and high-rate capabilities, delivering a Na + storage capacity of 260 mAh g −1 at 1.0 A g −1 after 1000 cycles, a Li + storage capacity of 556 mAh g −1 at 0.5 A g −1 after 500 cycles, and a K + storage capacity of 198 mAh g −1 at 0.5 A g −1 after 400 cycles.
研究领域:
材料科学
·
电化学
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纳米技术
·
能量存储
✓ 已验证
DOI
高影响因子
Journal of the Taiwan Institute of Chemical Engineers
·
2021
·
IF 5.876
Yanfang Chen,Xuemei Yan,Haifeng Lin,Chao Wang,Jixiang Xu
Background Fe 2+ /H 2 O 2 -based photo-Fenton process is often used in pollutions removal. But, Fe-species related low activity at neutral condition hinders their practical applications. Activation of in situ generated H 2 O 2 for Fenton-like degradation of pollutions through catalysts absences of Fe species is expected. Methods A strip-like benzene and K + co-doped g-C 3 N 4 (KBCN) was constructed by the thermal polymerization of assembly of melamine and 2-aminoterephthalic acid, then post-calcination in LiCl–KCl molten salt, which was then used to degrade Rhodamine B (RhB) and Congo red (CR) via in situ generated H 2 O 2 . Significant findings Benzene and K + co-doping were critical both in increasing charge separation and enhancing the H 2 O 2 production. The yield of H 2 O 2 for KBCN was 57.5 μM in pure water after 60 min irradiation. In the absence of Fe 2+ , the in situ generated H 2 O 2 could subsequently degrade RhB and CR with the formed ·OH. The apparent rate constants of KBCN for RhB and CR degradation were 0.0446 and 0.0497 min −1 , respectively. The advantages of degradation with in situ generated H 2 O 2 in the absence of Fe 2+ can provide a new prospective to the environmental friendly Fenton-like reaction.
研究领域:
环境科学
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化学工程
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2023
·
IF 8.2
Xinghui Wu,Lina Yang,Mingjie Xia,Kejin Yu,Wenqi Cai,Taiyuan Shi,Mengxi Xie,He Liu
The stable energy barrier of mucin and soy hull polysaccharide (SHP) is established at the air/water interface in the intestinal fluid and is conducive to the absorption and transportation of nutrients. This study aimed to investigate the effect of different concentrations (0.5 % and 1.5 %) of Na + and K + on the energy barrier through the digestive system model in vitro . The interaction between ions and microwave-assisted ammonium oxalate-extracted SP (MASP)/mucus was characterized by particle size, zeta potential, interfacial tension, surface hydrophobicity, Fourier transform infrared spectroscopy, endogenous fluorescence spectroscopy, microstructure, and shear rheology. The results showed that the interactions between ions and MASP/mucus included electrostatic interaction, hydrophobic interaction, and hydrogen bond. The MASP/mucus miscible system was destabilized after 12 h, and the ions could improve the system stability to some extent. MASP aggregated continuously with the increase in the ion concentration, and large MASP aggregates were trapped above the mucus layer. Furthermore, the adsorption of MASP/mucus at the interface increased and then decreased. These findings provided a theoretical basis for an in-depth understanding of the mechanism of action of MASP in the intestine.
研究领域:
食品科学
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营养学
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生物物理学
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产品关联
DOI
PubMed
高影响因子
Advanced Healthcare Materials
·
2023
·
IF 10
Panqin Ma,Zheng Luo,Zhiguo Li,Yuchao Lin,Zibiao Li,Zhen Wu,Changliang Ren,Yun-Long Wu
Mitochondrial potassium ion channels have become a promising target for cancer therapy. However, in malignant tumors, their low expression or inhibitory regulation typically leads to undesired cancer therapy, or even induces drug resistance. Herein, this work develops an in situ mitochondria-targeted artificial K + channel construction strategy, with the purpose to trigger cancer cell apoptosis by impairing mitochondrial ion homeostasis. Considering the fact that cancer cells have a lower membrane potential than that of normal cells, this strategy can selectively deliver artificial K + channel molecule 5F8 to the mitochondria of cancer cells, by using a mitochondria-targeting triphenylphosphine (TPP) modified block polymer (MPTPP) as a carrier. More importantly, 5F8 can further specifically form a K + -selective ion channel through the directional assembly of crown ethers on the mitochondrial membrane, thereby inducing mitochondrial K + influx and disrupting ions homeostasis. Thanks to this design, mitochondrial dysfunction, including decreased mitochondrial membrane potential, reduced adenosine triphosphate (ATP) synthesis, downregulated antiapoptotic BCL-2 and MCL-1 protein levels, and increased reactive oxygen species (ROS) levels, can further effectively induce the programmed apoptosis of multidrug-resistant cancer cells, no matter in case of pump or nonpump dependent drug resistance. In short, this mitochondria-targeted artificial K + -selective ion channel construction strategy may be beneficial for potential drug resistance cancer therapy.
研究领域:
癌症生物学
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纳米技术
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药学
·
细胞生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Nano
·
2025
·
IF 16
Jue-Ying Gong,Yao Li,Po Wang,Xu Peng,Rui Xie,Wei Wang,Zhuang Liu,Da-Wei Pan,Xiao-Jie Ju,Liang-Yin Chu
Intracellular bacteria can evade the attack of the immune system and the bactericidal effects of most antibiotics due to the protective effect of the host cells. Herein, inspired by the stimuli-responsive behaviors of biological ion channels, a kind of synergistic cascade potassium ion (K+)-responsive nanoparticles gated with K+-responsive polymers is ingeniously designed to target intracellular bacteria and then control drug release. Due to the cooperative interaction of host–guest complexation and conformational transition of K+-responsive polymers, the grafted gates based on these polymers could recognize high K+ concentration to reverse the negatively charged nanoparticles into positively charged ones for targeting bacteria and subsequently inducing a switch from the hydrophobic shrinking “off” state to the hydrophilic stretching “on” state for drug release. The K+-responsive nanoparticles can effectively load antibiotics and be endocytosed into the infected cells, and K+-responsive gates can be actuated by a high intracellular K+ concentration. With the efficient synergistic cascade strategy, these K+-responsive nanocarriers can deliver antibiotics to the subcellular region where intracellular bacteria reside and show superior elimination efficiencies in vitro and in vivo than the free drug in delivering vancomycin. The K+-responsive nanocarriers are expected to improve the bioavailability of drugs and enhance their antibacterial efficacy.
研究领域:
纳米技术
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药学
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细菌学
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药物递送系统
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材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF PHARMACY AND PHARMACOLOGY
·
2016
·Vol. 68
·期 9
·1109-1118
·
IF 2.363
Yang Lei,Xin Jin,Zhang Zhenhai,Yan Hongmei,Wang Jing,Sun E,Hou Jian,Jia Xiaobin,Lv Huixia
ObjectiveThis work aimed at preparing ginsenoside compound K (GCK)-loaded liposomes modified with TPGS (GCKT-liposomes) to enhance solubility and targeting capability of GCK, as well as inhibit the efflux of GCK from tumour cells.MethodsGCKT-liposomes were prepared by the thin-film hydration method and characterized by particle size, polydispersity, zeta potential and drug encapsulation efficiency. A549 cells were used as antitumour cell model to access the cellular uptake of the GCK and perform its antitumour function. The enhancement of in vivo antitumour efficacy of GCKT-liposomes was evaluated by nude mice bearing tumour model.Key findingsThe results showed that GCKT-liposomes achieved a comparatively high drug loading efficiency and reasonable particle size at the ratio of 7 : 3 (phospholipid: TPGS). The in vitro release demonstrated that the dissolution of GCK was remarkably improved by entrapping it into liposomes. In addition, GCKT-liposomes exhibited a great hypersensitizing effect on A549 cells, and the cellular uptake was enhanced. Compared with free GCK, the IC50 of GCKT-liposomes was significantly reduced (16.3 ± 0.8 vs 24.9 ± 1.0 μg/ml). In vivo antitumour assay also indicated that GCKT-liposomes achieved higher antitumour efficacy (67.5 ± 0.5 vs 40.8 ± 0.7%).ConclusionThe novel GCKT-liposomes significantly improved the antitumour efficacy of GCK.
研究领域:
药学
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肿瘤学
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药物递送系统
·
癌症研究
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
·
2024
·
IF 8.1
Lina Wang,Hanpei Yang,Qiangshun Wu,Rui Ma
Photocatalysis-self-Fenton system, integrating photocatalysis with Fenton technology, emerge as promising approaches for environmental remediation. In this study, we introduced K, Na doped g-C 3 N 4 /FeOCl (DCN/FeOCl) catalyst, where amino groups on the DCN surface interacted with unsaturated Fe on FeOCl, forming Fe–N bonds and a Z-scheme heterojunction . The resulting DCN/FeOCl demonstrated efficient H 2 O 2 production and rapid tetracycline (TC) degradation under visible light. The Z-scheme heterojunction and nitrogen vacancy enhanced electron-hole pair separation and O 2 adsorption. Additionally, we proposed a mechanism for dual-channel photocatalytic H 2 O 2 production. This work presents a novel strategy for in-situ H 2 O 2 production and activation, facilitating water decontamination .
研究领域:
环境科学
·
材料科学
·
化学工程
✓ 已验证
产品关联
DOI
高影响因子
INFLAMMATION RESEARCH
·
2023
·
IF 6.7
He Ze-Hui,Zou Jing-Tao,Chen Xia,Gong Jiang-Shan,Chen Ya,Jin Ling,Liu Yi-Wei,Rao Shan-Shan,Yin Hao,Tan Yi-Juan,Wang Zun,Du Wei,Li Hong-Ming,Qian Yu-Xuan,Wang Zhen-Xing,Wang Yi-Yi,Wan Teng-Fei,Luo Yi,Zhu Hao,Chen Chun-Yuan,Xie Hui
Objective Nanoparticles (NPs) hold a great promise in combating rheumatoid arthritis, but are often compromised by their toxicities because the currently used NPs are usually synthesized by chemical methods. Our group has previously fabricated Ångstrom-scale silver particles (AgÅPs) and demonstrated the anti-tumor and anti-sepsis efficacy of fructose-coated AgÅPs (F-AgÅPs). This study aimed to uncover the efficacy and mechanisms of F-AgÅPs for arthritis therapy. Methods We evaluated the efficacy of F-AgÅPs in collagen-induced arthritis (CIA) mice. We also compared the capacities of F-AgÅPs, the commercial AgNPs, and the clinical drug methotrexate (MTX) in protecting against K/BxN serum-transfer arthritis (STA) mice. Moreover, we evaluated the effects of F-AgÅPs and AgNPs on inflammation, osteoclast formation, synoviocytes migration, and matrix metalloproteinases (MMPs) production in vitro and in vivo. Meanwhile, the toxicities of F-AgÅPs and AgNPs in vitro and in vivo were also tested. Results F-AgÅPs significantly prevented bone erosion, synovitis, and cartilage damage, attenuated rheumatic pain, and improved the impaired motor function in mouse models of CIA or STA, the anti-rheumatic effects of which were comparable or stronger than AgNPs and MTX. Further studies revealed that F-AgÅPs exhibited similar or greater inhibitory abilities than AgNPs to suppress inflammation, osteoclast formation, synoviocytes migration, and MMPs production. No obvious toxicities were observed in vitro and in vivo after F-AgÅPs treatment. Conclusions F-AgÅPs can effectively alleviate arthritis without notable toxicities and their anti-arthritic effects are associated with the inhibition of inflammation, osteoclastogenesis, synoviocytes migration, and MMPs production. Our study suggests the prospect of F-AgÅPs as an efficient and low-toxicity agent for arthritis therapy.
研究领域:
风湿病学
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纳米技术
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免疫学
·
药理学
✓ 已验证
DOI
PubMed
高影响因子
International Journal of Agronomy
·
2020
·
IF 0
Birhanu Kahsay Meresa,Hailay Mehari Gebremedhin
The diploid cool-season legume species, faba bean (Vicia faba L.), is one of the vital pulses for the people in the world as it maintains the sustainability of agriculture and provides nutrient-rich grains. Biotic and abiotic stresses are, however, challenging the faba bean production in many countries. The foliar diseases of faba bean are among the major constraints for the production and productivity of faba bean. Recently, a new foliar disease “Faba bean gall” caused by plant debris and soil-borne pathogen (Olpidium viciae K.) is rapidly spreading and causing high yield losses in Ethiopia. This review paper presents the distribution, impact, epidemiology, and biology of faba bean gall pathogen and principally illustrates how the application and investigation of the various diseases’ management approaches such as avoiding crop residues, crop rotation, intercropping, use of elicitors to induce host resistance, use of resistant genotypes, bioagents, compost teas, plant extracts, and lastly use of chemical fungicides could be important to control the faba bean gall disease underlying in faba bean.
研究领域:
农业
·
植物病理学
·
作物科学
·
生物安全
✓ 已验证
DOI
JOURNAL OF MOLECULAR LIQUIDS
·
2021
·
IF 6.165
Xiaofang Niu,Han Liu,Su Xue,Jinli Zhang,Wei Li
The solubility data of flonicamid (FA) in twelve mono-solvents (methanol (MA), ethanol (EA), n-propanol (NPA), isopropanol (IPA), n-butanol (NBA), isobutanol (IBA), ethyl acetate (EAC), butyl acetate (BAC), acetone (AT), acetonitrile (AN), tetrahydrofuran (THF), 1,4-dioxane (DX)) and a binary solvent of EA + EAC were experimentally measured by using a gravimetric method under atmospheric pressure. The tested temperature range for the studied solvents was from 283.15 K to 323.15 K except of 288.15–323.15 K for DX. Powder X-ray diffraction (PXRD) was applied to evaluate the crystal form of FA, and no solvate or new form formation were observed during the whole experiment. The experimental results show that the rising temperature leads to the increase of the FA solubility in all the studied mono and mixed solvents at the tested ranges. For mono-solvents, the solubility of FA at 293.15 K increases in the order: IBA (0.002641) < IPA (0.004243) < NPA (0.004361) < NBA (0.004750) < BAC (0.006844) < EA (0.006864) < EAC (0.01342) < MA (0.01394) < DX (0.01747) < AN (0.02455) < THF (0.03433) < AT (0.04645). For EA + EAC mixed solvents, the solubility of FA increases and then decreases with the rising mole fraction of EAC. That means addition of EAC to EA can alter the FA solubility. Density functional theory (DFT) calculations were performed to analyze the interactive energy between FA and the solvent molecules. Furthermore, the Apelblat equation, van’t Hoff equation, λh equation are applied to correlate the experimental solubility data of FA in mono and mixed solvent systems, while NRTL model and Wilson model are selected for mono-solvents, CNIBS/R-K model and Jouyban-Acree model for mixed solvents. Correlated results demonstrate that the Apelblat model provides the best regression. In addition, the dissolution thermodynamic properties of FA in chosen solvents are analyzed by the van’t Hoff equation. It suggests that the dissolution processes of FA are non-spontaneous, endothermic and entropy increase in these studied systems.
研究领域:
化学
·
物理化学
·
热力学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）