Bioactive Materials
·
2025
·
IF 20.3
Guoqing Zhong,Yali Miao,Jielong Zhou,Yijie He,Wenjie Yang,Chongquan Huang,Yunhui Zhang,Jin Xiao,Bingqing Bai,Jiaqi Zhou,Renshan Li,Tiantian Wei,Yu Zhang,Shi Cheng
Approximately half of lung cancer patients experience bone metastasis, leading to bone loss, fracture, and other skeletal-related events. Although immunotherapies have significantly advanced the therapeutic landscape for lung cancer, bone metastases have an immunologically "cold" microenvironment, representing a challenging obstacle when treating lung cancer. The combination of immunotherapy and photothermal therapy (PTT) for treating tumor-induced bone defects holds promise for enhancing the efficacy of local tumor ablation and inhibiting tumor recurrence and metastasis through activating systemic immune responses. Herein, we developed an injectable hydrogel-based photothermal immunotherapy system (BP@Gel-CD[SA] hydrogel) incorporating STING agonists (SA) and black phosphorus nanosheets (BPNSs) for high-efficiency tumor elimination, immune activation, and bone regeneration. The photothermal and photodynamic activities of BPNSs induce hyperthermia and ROS-mediated apoptosis of tumor cells. Meanwhile, SA loaded into the nano-boxes in BP@Gel-CD[SA] hydrogel by host-guest interaction significantly activates the cGas-STING pathway. It stimulates immunogenic cell death (ICD), synergistically promoting immune cell infiltration. Single-cell RNA sequence analysis confirms the modulation of the tumor microenvironment (TME) through the PTT-mediated ICD effect and the transactivation of the cGAS-STING pathway in immune cells of the TME. More importantly, the system can significantly inhibit the growth of distant tumors via systemic immune activation and elicit long-term immune memory in addition to tumor eradication. In the long term, this hydrogel system can promote the formation of new bone at sites of tumor-induced bone destruction, improving bone strength in the affected area. Collectively, this strategy provides an effective and safe option for treating lung cancer bone metastases.
研究领域:
肿瘤学
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生物材料学
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免疫学
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产品关联
DOI
PubMed
高影响因子
Materials Today Bio
·
2025
·
IF 10.2
Zili Guo,Tao Chen,Xuehuan Wen,Qiangchang Fu,Jiabin Lai,Jiajin Li,Jiayu Chen,Jie Weng,Wang Wen,Qihua Cao,Junxiong Zhu,Zhaoming Ye,Xiaohua Yu,Lifeng Jiang
Osteoarthritis (OA) pathogenesis is profoundly influenced by dysregulated immune dynamics, where persistent interleukin-17 (IL-17)/T helper 17 (Th17) cell mediated inflammation coordinates with failed regenerative processes to perpetuate joint destruction. Here, we unveil the role of myeloid-derived suppressor cells (MDSCs) as dual-phase regulators that paradoxically orchestrate both inflammatory escalation and tissue repair in OA progression. Intra-articular administration of MDSCs in OA mice amplified IL-17 dependent inflammatory cascades and chemokine-driven leukocyte recruitment, revealing a context-dependent pro-inflammatory phenotype. Unexpectedly, MDSC depletion failed to attenuate joint damage, implying their indispensable yet multifaceted role in OA pathogenesis. Mechanistically, MDSCs exhibited functional plasticity by upregulating arginase-1 to polarize M2 macrophages, fostering a regenerative niche alongside their inflammatory activity. To resolve this duality, we developed a bio-responsive hydrogel-microsphere system integrating transforming growth factor β1 (TGF-β1) and interleukin-1 β1 antibody (anti–IL-1β) loaded mesoporous silica nanoparticles (MSNs). This spatiotemporally controlled platform selectively suppressed MDSC-mediated Th17 cell expansion while harnessing their intrinsic capacity to drive M2 macrophage polarization and chondrogenesis. The resultant shift from a pro-inflammatory to pro-regenerative microenvironment significantly attenuated cartilage erosion and restored joint integrity in OA models. Our findings redefine MDSCs as bifunctional immune orchestrators in OA and establish precision biomaterial guided immune decoding as a paradigm-shifting therapeutic strategy. By engineering MDSC plasticity through antagonistic cytokine delivery, this work provides a blueprint for microenvironment remodeling in degenerative joint diseases.
研究领域:
免疫学
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再生医学
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生物材料
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纳米医学
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风湿病学
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组织工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Small
·
2025
·
IF 12.1
XiaoXiao Li,Xingchen Li,Zehao Chen,Xiaojian Ye,Lianfu Deng,Xiangyang Xu,Wenguo Cui
The simultaneous integration of high elasticity and lubricity-hallmarks of biological tissues-remains a fundamental challenge in synthetic hydrogels due to the intrinsic trade-off between “dehydration-induced” elasticity and “hydration-dependent” lubrication. Herein, inspired by the dynamic architecture of living systems, the construction of “living” biodegradable hydrogel microspheres is reported that reconcile this contradiction through internal nano-reinforcement and external molecular lubrication. Crystalline disc-like Laponite nanosheets are intercalated within GelMA networks, acting as dynamic, spatially confining crosslinkers that inhibit water infiltration and preserve network cohesion. Concurrently, zwitterionic brushes are grafted onto the microsphere surface, forming a robust hydration layer via dynamic charge–dipole interactions to enable long-lasting lubrication. This synergistic design endows the microspheres with tunable elasticity (14–4000 Pa) and adjustable friction coefficients (0.12–0.04), achieving a functional convergence of mechanical resilience and surface lubricity. Experimental evaluations confirm their efficacy in inhibiting excessive mechanical stress-induced calcium ion influx and downstream calcium signaling to prevent chondrocyte damage. This work offers a universal strategy to overcome the elasticity–lubrication paradox in hydrogels, unlocking their potential in biomedical engineering, drug delivery, and soft robotic interfaces.
研究领域:
生物材料学
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组织工程
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高分子科学
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生物医学工程
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软物质物理
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摩擦生物学
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药物递送系统
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Guangxin Shen,Botao Gao,Jiayi Guo,Weikang Xu,Guangfu Chen,Shuai Huang,Zhiwen Zeng,Xiaodong Zhao
Due to the limited ability to self-repair, the regeneration of bone critical-sized defects (CSD) is a significant challenge. Bone tissue engineering scaffolds are considered promising candidates for CSD repair, but low cell infiltration efficiency and a lack of nutrients greatly restrict bone regeneration abilities. Herein, we developed a dynamic culturing of large biomimetic bone scaffolds, PCL/GelMA@cells that combining 3D printed polycaprolactone (PCL) multi-channel cylinder with gelatin methacryloyl (GelMA) encapsulated with bone marrow mesenchymal stem cells (BMSCs) and rat aortic endothelial cells (RAECs). A cell dynamic culture system was fabricated to simulate the dynamic microenvironment. Compared to static culturing, dynamic culturing proved to enhance the nutrient exchange within the large scaffold to promote the cells infiltration, growth, proliferation and induce osteogenic and angiogenic differentiation. Furthermore, a rat cranial CSD (D = 10 mm) repair model verified the accelerated vascular ingrowth and new bone formation with the implantation of dynamic culturing of PCL/GelMA@cells scaffold (∼10 times higher than Blank group), indicating the great potential of dynamical culturing of scaffolds for bone repair. In summary, the results highlight the significant advantages of the dynamical culturing of cell-loaded scaffolds for bone regeneration, offering a promising strategy for addressing critical size bone defects.
研究领域:
生物医学工程
·
再生医学
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产品关联
DOI
PubMed
高影响因子
Bioactive Materials
·
2024
·
IF 18
Changjiang Liu,Kun Liu,Dong Zhang,Yuting Liu,Yifeng Yu,Haifei Kang,Xianzhen Dong,Honglian Dai,Aixi Yu
Diabetic wounds present multiple functional impairments, including neurovascular dysregulation, oxidative imbalance, and immune dysfunction, making wound healing particularly challenging, while traditional therapeutical strategies fail to address these complex issues effectively. Herein, we propose a strategy utilizing dual-layer microneedles to deliver therapeutic gases by modulating neurovascular coupling and immune functions for diabetic wound treatment. The microneedle can respond to reactive oxygen species (ROS) in the diabetic microenvironment and subsequently generate oxygen (O 2 ) and nitric oxide (NO). These gases comprehensively promote neuro-vascular regeneration, reduce oxidative stress levels, and attenuate inflammation. In vivo studies demonstrate that the microneedle can accelerate diabetic wound healing by modulating neurovascular regeneration and inflammatory processes. Transcriptomic analyses further validate the involvement of related advantageous signaling pathways. The potential mechanism involves the activation of the PI3K-AKT-mTOR pathway to facilitate autophagy, ultimately accelerating the healing process. Thus, our multifunctional dual-layer microneedles provide an effective strategy for treating diabetic wounds.
研究领域:
生物医学工程
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药理学
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伤口愈合
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糖尿病研究
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神经科学
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免疫学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Tissue Engineering Part C-Methods
·
2024
·Vol. 30
·期 3
·113-129
·
IF 2.7
Xiaoxiao Li,Weikang Zhao,Dandan Zhou,Pei Li,Chen Zhao,Qiang Zhou,Yiyang Wang
The decellularized extracellular matrix (ECM) of cartilage is a widely used natural bioscaffold for constructing tissue-engineered cartilage due to its good biocompatibility and regeneration properties. However, current decellularization methods for accessing decellularized cartilaginous tissues require multiple steps and a relatively long duration to produce decellularized cartilage. In addition, most decellularization strategies lead to damage of the microstructure and loss of functional components of the cartilaginous matrix. In this study, a novel decellularization strategy based on a hydrostatic pressure (HP) bioreactor was introduced, which aimed to improve the efficiency of producing integral decellularized cartilage pieces by combining physical and chemical decellularization methods in a perfusing manner. Two types of cartilaginous tissues, auricular cartilage (AC) and nucleus pulposus (NP) fibrocartilage, were selected for comparison of the effects of ordinary, positive, and negative HP-based decellularization according to the cell clearance ratio, microstructural changes, ECM components, and mechanical properties. The results indicated that applying positive HP improved the efficiency of producing decellularized AC, but no significant differences in decellularization efficiency were found between the ordinary and negative HP-treated groups. However, compared with the ordinary HP treatment, the application of the positive or negative HP did not affect the efficiency of decellularized NP productions. Moreover, neither positive nor negative HP influenced the preservation of the microstructure and components of the AC matrix. However, applying negative HP disarranged the fibril distribution of the NP matrix and reduced glycosaminoglycans and collagen type II contents, two essential ECM components. In addition, the positive HP was beneficial for maintaining the mechanical properties of decellularized cartilage. The recellularization experiments also verified the good biocompatibility of the decellularized cartilage produced by the present bioreactor-based decellularization method under positive HP. Overall, applying positive HP-based decellularization resulted in a superior effect on the production of close-to-natural scaffolds for cartilage tissue engineering.Impact statementIn this study, we successfully constructed a novel hydrostatic pressure (HP) bioreactor and used this equipment to produce decellularized cartilage by combining physical and chemical decellularization methods in a perfusing manner. We found that positive HP-based decellularization could improve the production efficiency of integral decellularized cartilage pieces and promote the maintenance of matrix components and mechanical properties. This new decellularization strategy exhibited a superior effect in the production of close-to-natural scaffolds and positively impacts cartilage tissue engineering.
研究领域:
组织工程
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生物医学工程
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软骨生物学
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机械工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOMACROMOLECULES
·
2024
·
IF 5.5
Yan Fang,Yukai Lin,Linyu Wang,Yunxiang Weng,Qinhui Chen,Haiqing Liu
Hemostatic powder is widely employed for emergency bleeding control due to its ability to conform to irregularly shaped wounds, ease of use, and stable storage. However, current powders exhibit limited tissue adhesion and insufficient support for thrombus formation, making them easily washed away by blood. In this study, a hybrid powder (QAL) was produced by mixing quaternized chitosan (QCS) powder, catechol-modified alginate (Cat-SA) powder, and laponite (Lap) powder. Upon addition of QAL, the blood quickly transformed to a robust and adhesive blood gel. The adhesion strength of the blood gel was up to 31.33 ± 1.56 kPa. When compared with Celox, QAL showed superior performance in promoting hemostasis. Additionally, QAL exhibited effectiveness in eliminating bacteria while also demonstrating outstanding biocompatibility with cells and blood. These favorable properties, including strong coagulation, adhesion to wet tissue, antibacterial activity, biosafety, ease of use, and stable storage, make QAL a promising emergency hemostatic agent.
研究领域:
生物医学工程
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOMATERIALS
·
2024
·
IF 12.8
Zeyuan Jin,Yaqi Zhang,Haijun Hu,Qian Li,Liwen Zhang,Kefei Zhao,Wenxing Liu,Lanjuan Li,Changyou Gao
Inflammatory bowel disease (IBD) is characterized by the upregulation of reactive oxygen species (ROS) and dysfunction of gut immune system, and microbiota. The conventional treatments mainly focus on symptom control with medication by overuse of drugs. There is an urgent need to develop a closed-loop strategy that combines in situ monitoring and precise treatment. Herein, we innovatively designed the ‘cluster munition structure’ theranostic microgels to realize the monitoring and therapy for ulcerative colitis (a subtype of IBD). The superoxide anion specific probe (tetraphenylethylene-coelenterazine, TPC) and ROS-responsive nanogels consisting of postbiotics urolithin A (UA) were loaded into alginate and ion-crosslinked to obtain the theranostic microgels. The theranostic microgels could be delivered to the inflammatory site, where the environment-triggered breakup of the microgels and release of the nanogels were achieved in sequence. The TPC-UA group had optimal results in reducing inflammation, repairing colonic epithelial tissue, and remodeling microbiota, leading to inflammation amelioration and recovery of tight junction between the colonic epithelium, and maintenance of gut microbiota. During the recovery process, the local chemiluminescence intensity, which is proportional to the degree of inflammation, was gradually inhibited. The cluster munition of theranostic microgels displayed promising outcomes in monitoring inflammation and precise therapy, and demonstrated the potential for inflammatory disease management.
研究领域:
药理学
·
生物医学工程
·
胃肠病学
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免疫学
·
微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Chaofan Yao,Qian Wu,Yu Zhao,Hongbin Li,Jinmei He,Li Liu,Yudong Huang,Feng Cheng
Wound bacterial infections not only impede the healing process but can also give rise to a range of serious complications, thereby posing a substantial risk to human health. Developing effective wound dressings incorporating phototherapy functionalities, specifically photothermal therapy (PTT) and photodynamic therapy (PDT), remains a critical area of research in modern wound care. Existing PTT-PDT systems often suffer from challenges such as nanoparticle aggregation and inefficient reactive oxygen species (ROS) generation, which are essential for therapeutic efficacy. This study presents a pioneering approach by combining zeolitic imidazolate framework derivatives (ZIFs) and Au NPs in a silk fibroin (SF) hydrogel for the first time. This combination not only prevents particle aggregation but also significantly enhances photothermal conversion efficiency and ROS generation capacity. The digital light processing (DLP) printability of our hydrogel allows for customized wound dressings tailored to individual patient needs, improving therapeutic efficacy. The hydrogel's effectiveness was evaluated through rigorous in vivo experiments, demonstrating enhanced antibacterial properties and accelerated wound healing. The biocompatibility of our hydrogel ensures its suitability for clinical applications, minimizing adverse reactions while promoting healing. A wound healing rate of 99.06 % represents a substantial improvement over the control groups, indicating markedly enhanced therapeutic efficacy. These findings underscore its multifunctionality in addressing infected wounds, presenting a promising strategy for facilitating the rapid healing of acute complex wounds in clinical applications.
研究领域:
生物医学工程
·
材料科学
·
医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Nano
·
2025
·
IF 16
Bingfang Wang,Ailin Lv,Haofan Wu,Bihan Guo,Yuhan Lu,Zhiqiang Chang,Yuqing Wu,Xiang Li,Qiuyu Yang,Jianfang Nie,Jing Wei,Qinjuan Ren,Daizong Ji,Ya Zhang,Menahem Y. Rotenberg,Yin Fang
Cardiovascular disease (CAD) is a major global public health issue, with mortality rates being significantly impacted by cold temperatures. Stable and reliable electrocardiogram (ECG) monitoring in cold environments is crucial for early detection and treatment of CAD. However, existing skin sensor struggle to balance freeze resistance, breathability, flexibility, conductivity and adhesion at cold temperatures. Here, we introduce a solvent cross-linking strategy and an in situ transfer method to prepare ultrathin bioionic gels, featuring a freezing point below −80 °C and a thickness of only 12.6 μm. The strong and abundant interactions between the ionic liquid solvent and the zwitterionic polymer effectively suppress low-temperature crystallization, forming a toughened and highly adhesive network structure. This network enables the in situ formation of an ultrathin morphology, which can be seamlessly transferred onto various substrates. Furthermore, the solvent-cross-linked network maintains a large interpolymer chain spacing, facilitating rapid ion transport pathways. Even at subzero temperatures, the gel maintains its multifunctionality, demonstrating tissue-like softness (34.6 kPa), high ionic conductivity (10.06 mS cm–1), excellent stretchability (360%), high transparency, robust adhesive strength (175.3 kPa) and interfacial toughness (1146 J m–2). Integrated into a flexible wearable device, the ultrathin gel ensures excellent skin conformity, user comfort, and high signal-to-noise ECG signal acquisition. Leveraging an artificial neural network, the system analyzes bradycardia ECG signals and achieves 96.88% accuracy in arrhythmia detection under cold conditions. This bioionic gel-based system presents a promising solution for early CAD diagnosis and prediction in extreme environments.
研究领域:
生物医学工程
·
心血管疾病诊断
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材料科学
·
人工智能辅助医疗
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF MEMBRANE SCIENCE
·
2024
·
IF 8.4
Yan Zhang,Jianlin Yang,Yali Zhao,Zhecun Wang,Gwo Sung Lai
Membrane separation has been acknowledged as an advanced technology to handle the oil spills. However, most of the superhydrophilic/underwater superhydrophobic membranes currently applied for oil/water separation would lose their superwettability when exposed to salty marine environments, leading to serious membrane plugging and separation performance deterioration. Herein, inspired by seawater fish and mussels, a simple, green, and versatile one-step co-deposition strategy is proposed for fabricating a ternary zwitterionic coating with strong surface hydration on diverse membranes, showing excellent anti-fouling properties in high salinity environments, even in the saturated NaCl solution. Moreover, the modified membrane performs well in the long term when separating surfactant-stabilized oil-in-seawater emulsion and with impressive recyclability. The ternary zwitterionic coating membrane exhibits great potential in treating oily wastewater in marine.
研究领域:
材料科学
·
环境科学
·
海洋生物学
✓ 已验证
产品关联
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2024
·
IF 13.3
Weijun He,Haofei Li,Xinbin Xu,Xing Zhang,Junlin Chen,Chuhan Lv,Hongbo Yu,Qi Feng,Hua Dong
Islets transplantation is a promising method to treat type 1 diabetes. Unfortunately, current islet encapsulation and transplantation strategies are difficult to simultaneously achieve the minimally invasive transplantation, rapid mass transportation, accurate positioning, long-term stability and easy retrieval. Herein, we present a novel strategy for islet encapsulation and transplantation that integrates the advantages of microencapsulation and macroencapsulation. Specifically, islets are first encapsulated in methacrylated carboxymethyl chitosan (CMCSMA) microgels by droplet microfluidic technology, and then oxidized sodium alginate-succinimide ester (OSA-NHS) as the assembling agent is added to time-sequentially assemble the islets-laden microgels. The as-formed microgel assembly (MA-CHO/NHS), or namely bioartificial pancreas, is injectable before transplantation due to the quick formation of dynamic Schiff base bonds, and becomes quite stable after transplantation with the slow formation of stable amide bonds. In addition, the interconnected micropores inside the microgel assembly is beneficial for mass transportation, and the abundant aldehyde and succinimide ester groups on assembling agent ensures good tissue adhesion. This injectable, tissue-adhesive, microporous, positionable and retrievable bioartificial pancreas can reverse the blood glucose levels of diabetic mice from hyperglycemia to normoglycemia for at least 90 days, and thus shows a broad application prospect in the field of diabetes treatment.
研究领域:
生物医学工程
·
糖尿病研究
·
组织工程
·
生物材料
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）