FOOD RESEARCH INTERNATIONAL
·
2026
·
IF 8.8
Jihui Gao,Tian Zheng,Yuyang Shi,Lei Jiang,Lei Huang,Bingsheng Qiu,Yongxin Li,Gang Chen,Jinping Si,Huqing Yang,Shangbin Bai
L. plantarum P5 improved the fermentation performance of PC-enriched FRCs. • L. plantarum P5 - assisted fermentation reduced off-flavors in PC-enriched FRCs. • The PCL group exhibited slower moisture migration, delaying starch retrogradation. • The PCL group predominantly formed V-type complexes, reducing starch digestibility. Although Polygonatum cyrtonema (PC) is rich in bioactive compounds, its direct incorporation may compromise the sensory quality of fermented products. This study systematically investigated the effects of PC on fermented rice cakes (FRCs), using Lactobacillus plantarum P5-assisted fermentation as a strategy to improve product quality. L. plantarum P5, as the optimal strain, significantly promoted yeast growth and increased total soluble solids in fermented glutinous rice, thereby improving the specific volume, height-to-diameter ratio, and textural characteristics of PC-enriched FRCs. SPME-GC–MS analysis showed that P5-assisted fermentation reduced off-flavor compounds, including hexanal and (E)-2-heptenal. Additionally, the group containing both PC and L. plantarum P5 (PCL) exhibited a slower staling rate and reduced moisture migration during storage. The resistant starch (RS) content in the PCL group significantly increased from 17.94% to 20.17%. X-ray diffraction analysis revealed that the PCL group exhibited predominantly V-type crystalline structures, indicating the development of inclusion complexes between bioactive compounds in PC and starch. Meanwhile, the PCL group also showed enhanced hydrogen bonding between PC polysaccharides (PCPs) and starch chains, promoting starch aggregation and the development of a more compact gel network. Overall, this study provides an approach for incorporating PC into fermented cereal products with enhanced product quality and reduced starch digestibility. Download: Download high-res image (363KB) Download: Download full-size image
✓ 已验证
DOI
高影响因子
Chemical and Biological Technologies in Agriculture
·
2026
·
IF 5.8
Tang Huazhi,Ye Zixin,Chen Mingsheng,Yang Xiaotong,Song Chengcheng,Tai Guihua,Zhou Yifa,Cui Sisi,Cui Liangnan
Background Probiotic strain Lactobacillus gasseri ( L. gasseri ) is widely used in the food industry due to its diverse bioactive activities, in which the exopolysaccharide (EPS) is a key functional component. While the secreted EPS from L. gasseri has been previously explored, its capsular form remains much less characterized, limiting the comprehensive understanding of EPS-derived postbiotics in this species. This study aims to isolate, purify, and structurally characterize the capsular exopolysaccharide from L. gasseri ATCC 33323. Results A capsular exopolysaccharide, designated C-EPS L.g -G, was isolated and purified from L. gasseri ATCC 33323. Structural characterization revealed that C-EPS L.g -G (Mw 36.1 kDa) is a highly branched, atypical dextran with a branching degree of 76.9%. It consists of an α-(1→6) backbone substituted at the α-(1→2) position by monomeric glucose side chains. These side chains have a defined arrangement with an unsubstituted/sparse central core that is symmetrically flanked by dense branches. Notably, 52.4% of the side chains carry 1-phosphoglycerol modifications at the O-6 position. Additionally, minor acetyl (8.3%) and 3-hydroxypropionyl (10.5%) modifications are present in C-EPS L.g -G at variable acylation sites. In 0.15 M NaCl solution, C-EPS L.g -G adopts a spherical conformation characterized by a small Z-average hydrodynamic diameter (7.64 ± 0.20 nm), moderate intrinsic viscosity (11.61 ± 0.21 mL/g), and slightly negative zeta potential (– 8.71 ± 0.32 mV). Comparative genomic analysis revealed that the biosynthetic gene locus for this atypical dextran is conserved across most L. gasseri and Lactobacillus johnsonii species. Conclusions This study reports the purification and comprehensive structural characterization of an atypical dextran-type capsular exopolysaccharide C-EPS L.g -G from L. gasseri ATCC 33323 for the first time. Resulting structural insights provide a foundation for future functional exploration and potential applications of this atypical dextran in the food industry. Graphical The alternative text for this image may have been generated using AI.
✓ 已验证
DOI
高影响因子
Cell Reports
·
2026
·Vol. 45
·期 6
·
IF 7.7
Jiarun Xu,Guoyue Wei,Jianfeng Wang,Haoran Wang,Jian He,Jiaqi Xu,Wenguo Cao,Jialin Wang,Qi Shen,Nasi Ai,Yufang Su,Huaxi Yi,Lingjun Tong
Pre-inflammatory bowel disease (pre-IBD) represents a transitional state between irritable bowel syndrome and IBD, characterized by heightened susceptibility to mucosal inflammation driven by chronic high-fat diet intake and antibiotic exposure. However, effective intervention strategies remain poorly defined. Here, we identify milk-derived extracellular vesicles (mEVs) as a potent nanotherapeutic capable of attenuating pre-IBD pathology. Pre-IBD mice displayed marked gut microbiota dysbiosis and mucosal inflammation, both of which were substantially ameliorated by oral mEVs administration. Mechanistically, mEVs restored microbial homeostasis by enriching Lactobacillus murinus ( L. murinus ), which in turn reduced lysophosphatidylcholine (LPC) accumulation, two factors that exerted opposing and functionally instructive effects on intestinal inflammation. LPC activated the TLR2/MAPK axis to promote M1 polarization, whereas L. murinus engaged the TLR2/STAT3 pathway to drive M2 polarization. Collectively, these findings demonstrate that mEVs reprogram TLR2-dependent macrophage responses by modulating LPC and L. murinus , thereby mitigating mucosal inflammation and preventing the progression of pre-IBD.
✓ 已验证
DOI
PubMed
高影响因子
npj Science of Food
·
2026
·
IF 9.7
Lingnv Yao,Siyuan Xie,Shuobo Zhang,Furong Ba,Huijuan Jing,Bo Qiu,Lvwan Xu,Shengyi Han,Hang Xiao,Ming Zhao,Xiaowei Shi,Lanjuan Li,Mingfei Yao
Premature ovarian insufficiency (POI) threatens women’s reproductive health. Emerging evidence implicates the gut-ovary axis in POI pathogenesis. This study explored the probiotic Lactobacillus salivarius Li01 in a Tripterygium wilfordii glycosides-induced POI mouse model. Li01 effectively mitigated POI progression by restoring hormone levels, increasing functional follicle numbers, and improving ovarian antioxidant function. These beneficial effects were primarily mediated through modulation of the gut microbiota and host tryptophan metabolism. Notably, a tryptophan-enriched diet alone did not improve POI. When combined with Li01, it synergistically amplified the kynurenine pathway. This shift promoted ovarian NAD⁺ production by upregulating the levels of NAMPT, while attenuating NAD⁺ depletion through downregulation of CD38 expression. The elevated NAD⁺ levels subsequently activated SIRT1 expression, stimulated the synthesis of SOD and other antioxidants, and inhibited the Caspase-1/IL‑1β inflammasome axis. These findings highlight Li01 as a potential POI therapy through microbiota-metabolic regulation, offering new mechanistic insights into gut-ovarian interactions.
✓ 已验证
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Rongmi Zhou,Fangfang Ni,Shiyi Wu,Xinyu Wang,Jing Ming,Lantong Zhang,Lijuan Dong,Zhijun Song,Gerui Ren,Min Huang,Shuangbin Xu,Qiong Zhang,Khaled F. El-Massry,Hujun Xie
Lactobacillus plantarum (LP) and curcumin (CUR) were co-encapsulated in sodium alginate– Lactobacillus plantarum– curcumin–gelatin (ALG–LP–CUR–GE) hydrogel beads to evaluate the synergistic function of probiotics and prebiotics. The encapsulation efficiency of LP and CUR peaked at 84.6 % and 94.7 %, respectively. Results of scanning electron microscope, Fourier transform infrared spectrometry, and X-ray diffraction confirmed the encapsulation of LP and CUR in the hydrogel beads. In vitro digestion suggested that both CUR and LP can achieve successful release in the simulated intestinal fluid. The release kinetics of CUR showed that it exhibited predominantly Fickian release and non-Fickian release in gastric fluid and intestinal fluid, respectively. The application of co-encapsulated hydrogel beads in mimic milk tea illustrated that the viable count of LP remained above the minimum recommended threshold of 10 7 CFU/mL, offering a promising avenue for the delivery of synergistic probiotic and prebiotic functionalities.
研究领域:
食品科学
·
生物技术
·
药学
·
微生物学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Food Bioscience
·
2025
·
IF 5.9
Nan Cui,Chongwei Yu,Feiwei Cao,Qinchao Ding,Huiling Pan,Yaqi Zeng,Daxi Ren
Inflammatory bowel disease (IBD) is a prevalent intestinal disorder that profoundly affects human and animal health. Emerging evidence highlights the therapeutic potential of lactic acid bacteria (LAB) in mitigating gastrointestinal pathologies. Prior studies have shown that Lactobacillus helveticus ZJUIDS18 exhibits favorable in vitro growth characteristics, and has the potential to alleviate colitis. This study aimed to investigate the therapeutic effect and mechanism of L. helveticus ZJUIDS18 on mice colitis induced by sodium dextran sulfate (DSS). Key findings demonstrated that L. helveticus ZJUIDS18 intervention can effectively alleviate the symptoms of colitis induced by DSS in mice, including inhibiting the weight loss, colon shortening, and reducing disease activity index score as well as colonic tissue lesions. It increased the expression of tight junction proteins (ZO-1, claudin-1, and occludin) and mucin (MUC2) in colon tissue and decreased the level of myeloperoxidase (MPO), malondialdehyde (MDA), and pro-inflammatory factors IL-6 in colon tissue. Moreover, L. helveticus ZJUIDS18 intervention restored intestinal dysbiosis by down-regulating the relative abundance of harmful genera, namely Parabacteroides, Escherichia-Shigella, and Bacteroides and up-regulating the relative abundance of Bifidobacterium and Erysipelotrichaceae_uncultured. It also significantly increased the content of short-chain fatty acids, including acetic acid and propionic acid. Metabolic profiling further identified the tryptophan metabolism pathway as a critical mediator of the strain's anti-inflammatory effects, with L. helveticus ZJUIDS18 effectively ameliorating DSS-induced metabolic dysregulation. In summary, L. helveticus ZJUIDS18 could be as a probiotic candidate for developing targeted therapeutic strategies against IBD.
研究领域:
微生物学
·
胃肠病学
·
免疫学
·
代谢组学
·
益生菌研究
✓ 已验证
产品关联
DOI
高影响因子
eFood
·
2025
·Vol. 6
·期 4
·e70074
·
IF 5.7
Yebo Wang,Ke Li,Wenting Zhu,Huijuan Xi,Meng Qi,Ping Chen,Hanyu Chen,Chun-e Zhang,Ying Luo
The bioproduction of selenium nanoparticles (SeNPs) is garnering increasing attention as an environmentally friendly method, given that many microorganisms inherently possess the ability to synthesize nano-selenium. In the present study, Lactobacillus bulgaricus ( L. bulgaricus ) was selected to convert sodium selenite to SeNPs, the optimum conversion conditions were finally established, the morphology and structure of SeNPs were characterized as uniform distribution spherical particles with average size of 92.4 nm, in combination with field emission scanning electron microscope (FE-SEM), transmission electron microscope (TEM), X-ray photoelectron spectroscopy (XPS), and Fourier transform infrared spectroscopy (FTIR). 16S rDNA sequencing and genomic sequencing revealed that SeNPs influenced the composition of the gut microbiota, the abilities of immune and metabolism in mice were improved significantly after SeNPs supplement. The results corroborated the potential of SeNPs synthesized from probiotic L. bulgaricus CICC 20247 in intestinal health promotion and various biomedical even food processing applications.
研究领域:
微生物学
·
纳米技术
·
益生菌
·
胃肠病学
·
生物医学工程
·
食品科学
✓ 已验证
产品关联
DOI
高影响因子
International Journal of Oral Science
·
2026
·Vol. 18
·期 1
·16
·
IF 17.6
Wang Yuqiang,Tang Ying,Huang Qianxiao,An Jiaxin,Zhou Yueli,Yang Hongye,Song Fangfang,Zhang Xianzheng,Huang Cui
The onset and progression of periodontitis are closely associated with subgingival dysbiosis and excessive localized oxidative stress. While some oral probiotics exhibit certain inhibitory effects on periodontitis-related pathogens, they often struggle to effectively colonize and antagonize these pathogens due to the complex oxidative stress at the site of periodontitis. In this study, we engineer Lactobacillus reuteri with a reactive oxygen species (ROS)-responsive adhesive polymer (phenylboric acid-dopamine-hyaluronic acid) (LR@PDH). In the periodontitis microenvironment, this polymer can consume ROS and then expose the phenolic hydroxyl group of dopamine, promoting the selective adhesion and colonization of Lactobacillus reuteri at the site of inflammation to antagonize pathogens. The results show that, compared to conventional probiotic therapy, inflammation-responsive adhesive Lactobacillus reuteri effectively alleviates local oxidative stress, reduces the abundance of pathogenic bacteria in the subgingival microbiome, and inhibits the progression of periodontitis. Additionally, its good biocompatibility and safety highlight its potential as a therapeutic approach for clinical treatment of periodontitis.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Integrative Neuroscience
·
2026
·Vol. 25
·期 5
·
IF 3.3
Yu Kong,Xinhuang Lv,Yao Yang,Qiyao Li,Conghui Dai,Xiaoou Lin,Jiaming Liu,Jing Li
Background:The probiotic Lactobacillus mucosae has been widely shown to have many positive effects. However, its neuroprotective effects and underlying mechanism in Alzheimer’s disease (AD) remain elusive.Methods:Male APP/PS1 mice were treated for 4 weeks with L. mucosae WMU007, followed by the evaluation of cognitive function, neuronal damage, amyloid-β (Aβ) deposition, and Tau phosphorylation. RNA-seq coupled with Gene Ontology (GO) enrichment analysis implicated L. mucosae WMU007 in modulating oxidative stress in this AD model. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis and qPCR were performed to identify the specific mechanism by which this probiotic suppresses oxidative stress in the pathogenesis of AD. In addition, we quantified the levels of classical oxidative stress markers, such as superoxide dismutase 2 (SOD2) and glutathione peroxidase 4 (GPX4). We also examined the expression of cannabinoid receptor type 2 (CB2) and its key downstream regulators in the redox pathway, namely nuclear factor erythroid 2-related factor 2 (Nrf2) and heme oxygenase 1 (HO-1), in both animal and cellular models.Results:Our results showed that treatment with L. mucosae WMU007 significantly decreased cognitive impairment, neuronal damage, Aβ deposits, and Tau phosphorylation in APP/PS1 mice. Activation of CB2 was identified as the key mechanism by which L. mucosae WMU007 reduces oxidative stress in AD. In addition, L. mucosae WMU007 reduced oxidative stress and increased the levels of CB2 pathway-related proteins in vivo and in vitro.Conclusions:These results indicate that L. mucosae WMU007 confers neuroprotection in AD by targeting CB2-mediated oxidative pathways, highlighting its therapeutic potential as a novel probiotic intervention.
✓ 已验证
DOI
PubMed
高影响因子
ENZYME AND MICROBIAL TECHNOLOGY
·
2019
·
IF 3.553
Fei-Long Li,Wen Bin Su,Qing-Lan Tao,Liao-Yuan Zhang,Ye-Wang Zhang
Enzyme-catalyzed cofactor regeneration is a significant approach to avoid large quantities consumption of oxidized cofactor, which is vital in a variety of bioconversion reactions. NADH: FMN oxidoreductase is an ideal regenerating enzyme because innocuous molecular oxygen is required as an oxidant. But the by-product H 2 O 2 limits its further applications at the industrial scale. Here, novel NADH: FMN oxidoreductase (LrFOR) from Lactobacillus rhamnosus comprised of 1146 bp with a predicted molecular weight of 42 kDa was cloned and overexpressed in Escherichia coli BL21 (DE3). Enzyme assay shows that the purified recombinant LrFOR has both the NADPH and NADH oxidation activity. Biochemical characterizations suggested that LrFOR exhibits the specific activity of 39.8 U·mg −1 with the optimal pH and temperature of 5.6 and 35 °C and produces H 2 O instead of potentially harmful peroxide. To further study its catalytic function, a critical Thr29 residue and its six mutants were investigated. Mutants T29G, T29A, and T29D show notable enhancement in activities compared with the wild type. Molecular docking of NADH into wild type and its mutants reveal that a small size or electronegative of residue in position29 could shorten the distance of NADH and FMN, promoting the electrons transfer and resulting in the increased activity. This work reveals the pivotal role of position 29 in the catalytic function of LrFOR and provides effective catalysts in NAD + regeneration.
研究领域:
生物化学
·
酶学
·
微生物学
✓ 已验证
DOI
PubMed
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2020
·
IF 4.006
Yuzhu Zhu,Jia-Min Zhou,Wei Liu,Xionge Pi,Qingqing Zhou,Ping Li,Tao Zhou,Qing Gu
Exopolysaccharides (EPS) produced by some bacteria have prebiotic potential and contribute to the regulation of the gut microbiota. Four types of EPS with different molecular composition from Lactobacillus rhamnosus ZFM231 were identified. In this work, we evaluated the potential of EPS on human gut microbiota by in vitro fermentation model. It was found that purified EPS fractions were degraded effectively by human gut microbiota. Gas chromatography (GC) assay revealed that EPS could be fermented to produce short chain fatty acids (SCFAs) by gut microbiota in human fecal samples from twelve healthy volunteers. 16S rDNA sequencing showed that EPS mainly increased the abundance of Ruminococcus , Dorea , Butyricicoccus , Blautia and others. The current research provides valuable new information on the mechanisms of absorption, utilization and functional activity of Lactobacillus rhamnosus EPS in the gastrointestinal tract of the human body.
研究领域:
微生物学
·
营养学
·
胃肠病学
✓ 已验证
DOI
高影响因子
Foods
·
2021
·Vol. 10
·期 3
·529
·
IF 4.35
Jinsong Wang,Lanming Chen
Probiotics are considered as desirable alternatives to antibiotics because of their beneficial effects on the safety and economy of farm animals. The protectant can ensure the viability of probiotics, which is the prerequisite of the beneficial effects. The objective of this study was to evaluate the effects of a novel nano-protectant containing trehalose, skim milk powder, phytoglycogen nanoparticles, and nano-phytoglycogen Pickering emulsions on the viability ofLactobacillus caseiK17 under different conditions. The results indicated that the optimal concentration of the carbohydrate substrate was determined to be 10% skim milk powder (w/w) instead of trehalose. The combination of 10% skim milk powder (w/w), 1% phytoglycogen nanoparticles (w/w), and 10% Pickering emulsions (w/w) was selected as the optimal component of the protectant. Trilayer protectants with an optimal component had a more significant protective effect on the bacteria than that of the monolayer and bilayer protectants, or the control in feed storage, freeze-drying, and simulated gastrointestinal environment. A scanning electron microscope was used to monitor the morphological characteristics of the protectants for different layers onL. casei. In conclusion, the trilayer protectant exhibited a substantial effect onL. caseiduring storage and consumption, which could be used in the feed and functional food.Keywords:Lactobacillus casei;protectants;probiotics;phytoglycogen nanoparticle;pickering emulsions
研究领域:
微生物学
·
食品科学
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）