Horticulturae
·
2025
·Vol. 11
·期 12
·1478
·
IF 3
Ling Su,Xinqi Li,Lixing Ning,Peng Shu,Qingyi Zhang,Zugen Liu,Xiong Peng,Huili Liu,Yuan Yuan,Dingbo Yuan,Guicheng Liu,Guangyong You,Junfang Chen,Xiaoman Liu,Yi Tao,Yanhong Feng,Jing Yang
Potato is globally recognized as the fourth most crucial staple food crop, trailing behind wheat, rice, and maize. Cadmium (Cd), a predominant heavy-metal pollutant in agricultural soils, demonstrates high biological toxicity and mobility. Therefore, exploring the genetic and molecular mechanisms underpinning cadmium tolerance in potato is of substantial theoretical and practical significance. In this research, an F2population composed of 170 families was established through the cross-breeding of homozygous diploid potato lines HD-5 (highly cadmium-tolerant) and M9 (cadmium-sensitive). Employing hydroponic cultivation, six traits, namely plant height (PH), root length (RL), shoot fresh weight (SFW), root fresh weight (RFW), chlorophyll content (SPAD), and nitrogen content (LNC), were measured in potato seedlings following a 9-day treatment with 40 mg·L−1CdCl2. By utilizing the high-density genetic map of this population for QTL mapping, a total of 35 genetic loci associated with cadmium tolerance in potato seedlings were identified. Notably, loci21 and loci22 on chromosome 9, loci29 on chromosome 10, and loci31 and loci33 on chromosome 12 were consistently detected across multiple environmental conditions. This reproducibility across environments suggests the phenotypic stability of these five loci, which are thus considered reliable and robust genetic determinants. In addition, transcriptome sequencing analysis of roots from parental lines HD-5 and M9 after cadmium treatment revealed that significantly differentially expressed genes between the two parents were associated with glutathione metabolism and photosynthesis. By integrating QTL mapping, transcriptome analysis, and gene annotation, we screened four candidate genes involved in cadmium tolerance regulation:DM8C09G01000(GST),DM8C09G01060(GST),DM8C09G02130(OXP1), andDM8C06G22960(PsaH). These findings provide molecular targets and a genetic basis for molecular breeding of cadmium-tolerant potato varieties.
研究领域:
植物学
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遗传学
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植物育种
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分子生物学
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环境胁迫生理学
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产品关联
DOI
高影响因子
ELECTROCHIMICA ACTA
·
2025
·
IF 5.6
Yu-Hua Xiao,Qiu-Yu Huang,Tian-Yu Zhao,Xin-Ran Li,Lian-Kui Wu,Fa-He Cao
Understanding phase-specific corrosion behavior in multiphase alloys is critical for elucidating localized corrosion mechanisms. In this study, an integrated microelectrochemical cell technique (MECT) and scanning electrochemical microscopy (SECM) approach was developed to investigate in-situ electrochemical activity and local chemical environments on 2205 duplex stainless steel. Using a custom-fabricated dual-channel pipet, spatially resolved potentiodynamic polarization (PDP) and electrochemical impedance spectroscopy (EIS) measurements were performed at the α (ferrite) and γ (austenite) phases. Austenite exhibited higher corrosion current density i corr (60.4 μA·cm -2 ), lower charge transfer resistance R ct (39.2 kΩ·cm²), and reduced low-frequency impedance ( |Z| 0.1 Hz = 19.4 kΩ·cm²), indicating its preferential dissolution as the local anode in micro-galvanic coupling. Complementary in-situ pH mapping using a solid-state W/WO x micro-sensor revealed localized acidification and alkalization zones after 22, 32, and 56 h of immersion in acidic NaCl solution (pH = 1.5), coinciding with the onset and propagation of pitting. This dual-technique strategy provides unprecedented phase-resolved insight into corrosion dynamics, linking structural, electrochemical, and chemical heterogeneities across microdomains. The findings underscore the effectiveness of MECT-SECM integration for mechanistic studies of localized corrosion in duplex stainless steels and other complex alloys.
研究领域:
材料科学
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电化学
·
腐蚀工程
·
显微学
·
表面科学
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产品关联
DOI
高影响因子
Jove-Journal of Visualized Experiments
·
2019
·
IF 1.108
Yafeng Zhang,Xun Liao,Peihua Luo,Kui Peng,Wenyi Ye,Tengxiang Lian,Qibin Ma,Hai Nian
In plant mitochondria, some steady-state transcripts have 5' triphosphate derived from transcription initiation (primary transcripts), while the others contain 5' monophosphate generated post-transcriptionally (processed transcripts). To discriminate between the two types of transcripts, several strategies have been developed, and most of them depend on presence/absence of 5' triphosphate. However, the triphosphate at primary 5' termini is unstable, and it hinders a clear discrimination of the two types of transcripts. To systematically differentiate and map the primary and processed transcripts stably accumulated in maize mitochondrion, we have developed a circular RT-PCR (cRT-PCR)-based strategy by combining cRT-PCR, RNA 5' polyphoshpatase treatment, quantitative RT-PCR (RT-qPCR), and Northern blot. As an improvement, this strategy includes an RNA normalization step to minimize the influence of unstable 5' triphosphate.In this protocol, the enriched mitochondrial RNA is pre-treated by RNA 5' polyphosphatase, which converts 5' triphsophate to monophosphate. After circularization and reverse transcription, the two cDNAs derived from 5' polyphosphatase-treated and non-treated RNAs are normalized by maize 26S mature rRNA, which has a processed 5' end and is insensitive to 5' polyphosphatase. After normalization, the primary and processed transcripts are discriminated by comparing cRT-PCR and RT-qPCR products obtained from the treated and non-treated RNAs. The transcript termini are determined by cloning and sequencing of the cRT-PCR products, and then verified by Northern blot.By using this strategy, most steady-state transcripts in maize mitochondrion have been determined. Due to the complicated transcript pattern of some mitochondrial genes, a few steady-state transcripts were not differentiated and/or mapped, though they were detected in a Northern blot. We are not sure whether this strategy is suitable to discriminate and map the steady-state transcripts in other plant mitochondria or in plastids.
研究领域:
植物学
·
分子生物学
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产品关联
DOI
PubMed
高影响因子
Journal of Integrative Agriculture
·
2016
·
IF 0.724
Ai-yong QI,Pei-pei ZHANG,Yue ZHOU,Zhan-jun YAO,Zai-feng LI,Da-qun LIU
Wheat leaf rust is a destructive foliar disease of common wheat ( Triticum aestivum L.) worldwide. The most effective, economical s to control the disease is growing resistant cultivars with adult plant resistance (APR). The Chinese wheat lines W014204 and Fuyu 3 showed high leaf rust resistance in the field. To identify leaf rust APR genes in the two lines, two mapping populations with 215 and 163 F 2:3 lines from the crosses W014204/Zhengzhou 5389 and Fuyu 3/Zhengzhou 5389, respectively, were phenotyped for leaf rust severities during the 2010–2011, 2011–2012 and 2012–2013 cropping seasons in the field at Baoding, Hebei Province, China. A total of 1 215 SSR markers were used to identify the quantitative trait loci (QTLs) for leaf rust APR in the two populations. In the W014204/Zhengzhou 5389 population, three QTLs were detected and designated as QLr.hbu-1BL.1, QLr.hbu-2BS.1 and QLr.hbu-7DS, and explained 2.9–8.4, 11.5–38.3 and 8.5–44.5% of the phenotypic variance, respectively; all the resistance alleles at these loci were derived from W014204. In the Fuyu 3/Zhengzhou 5389 population, three QTLs, QLr.hbu-1BL.2, QLr.hbu-2BS.2 and QLr.hbu-7BL, explained 12.0–19.2, 22.3–38.9 and 4.1–4.3% of the phenotypic variance, respectively, and all resistance alleles were contributed by Fuyu 3. Based on chromosome positions of closely linked markers, both QLr.hbu-1BL.1 and QLr.hbu-1BL.2 are Lr46, and QLr.hbu-7DS is Lr34. QLr.hbu-7BL was mapped on chromosome 7BL near to Lr68 and they are likely the same gene. Based on chromosome positions, pedigree and field reactions, the two 2BS QTLs are different from all the known APR genes and are likely to be new APR QTL for leaf rust. These QTLs and their closely linked markers are potentially useful for improving leaf rust resistance in wheat breeding.
研究领域:
植物学
·
遗传学
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产品关联
DOI
高影响因子
ACS Applied Bio Materials
·
2021
·
IF 0
Xinrong Li,Yilin Song,Guihua Xiao,Enhui He,Jingyu Xie,Yuchuan Dai,Yu Xing,Yun Wang,Yiding Wang,Shengwei Xu,Mixia Wang,Tiger H. Tao,Xinxia Cai
Epilepsy detection and focus location are urgent issues that need to be solved in epilepsy research. A cortex conformable and fine spatial accuracy electrocorticogram (ECoG) sensor array, especially for real-time detection of multicortical functional regions and delineating epileptic focus remains a challenge. Here, we fabricated a polydimethylsiloxane (PDMS)–parylene hybrid, flexible micro-ECoG electrode array. The multiwalled carbon nanotubes (MWCNTs)/poly(3,4-ethylenedioxythiophene):poly(styrene sulfonate) (PEDOT:PSS) nanocomposite-modified electrode interface significantly improved the sensing performance with low impedance (20.68 ± 6.65 kΩ), stable phase offset, and high sensitivity. The electrophysiological activities of multicortical brain regions (somatosensory cortex, parietal association cortex, and visual cortex) were simultaneously monitored during normal and epileptic statuses. The epileptic ECoG activities spread spatiotemporally from the starting point toward the adjacent cortex. Significant variations of the waveform, power, and frequency band were observed. The ECoG potential (123 ± 23 μV) at normal status was prominently up to 417 ± 87 μV at the spike wave stage. Besides, the power for epileptic activity (11.049 ± 4.513 μW) was 10 times higher than that (1.092 ± 0.369 μW) for normal activity. In addition, the theta frequency band was found to be a characteristic frequency band of epileptic signals. These joint analysis results of multicortical regions indicated that the active micron-scale region on the parietal association cortex was more likely to be the epileptogenic focus. Cortical mapping with high spatial detail provides the accurate delineation of lesions. The flexible micro-ECoG electrode array is a powerful tool for constructing a spatiotemporal map of the cortex. It provides a technical platform for epileptic focus location, biomedical diagnosis, and brain–computer interaction.
研究领域:
神经科学
·
生物医学工程
·
材料科学
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产品关联
DOI
PubMed
ANALYTICAL CHEMISTRY
·
2022
·
IF 8.008
Changxiang Fang,Junying Li,Zhihao Feng,Xiaoyi Li,Min Cheng,Yan Qiao,Weihua Hu
Extracellular electron transfer (EET) is a critical process involved in microbial fuel cells. Spatially resolved mapping of EET flux is of essential significance due to the inevitable spatial inhomogeneity over the bacteria/electrode interface. In this work, EET flux of a typical bioanode constructed by inhabiting Shewanella putrefaciens CN32 on a porous polyaniline (PANI) film was successfully mapped using a newly established oblique incident reflectivity difference (OIRD) technique. In the open-circuit state, the PANI film was reduced by the electrons released from the bacteria via the EET process, and the resultant redox state change of PANI was sensitively imaged by OIRD in a real-time and noninvasive manner. Due to the strong correlation between the EET flux and OIRD signal, the OIRD differential image represents spatially resolved EET flux, and the in situ OIRD signal reveals the dynamic behavior during the EET process, thus providing important spatiotemporal information complementary to the bulky electrochemical data.
研究领域:
生物工程
·
电化学
·
微生物学
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产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2026
·
IF 7.3
Zhihao Zhou,Kangrui Hu,Nan Jia,Zhongbo Wang,Ke Ding,Qi Chen,Yiyue Yan,Lin Su,Xiayu Qian,Xin Fan,Lin Xie,Guangji Wang,Tengjie Yu,Yan Liang
Mass spectrometry imaging (MSI) can directly detect surface analytes, enabling spatial mapping of metabolite synthesis and migration within tissues. However, MSI often lacks the sensitivity for routine analysis of low exposure, low molecular weight endogenous metabolites. Amine-containing metabolites, such as methylamine, dimethylamine, trimethylamine, lysine, dopamine, and 5-hydroxytryptamine, are widely used as medical biomarkers due to their potential biological activity or toxicity. Herein, we innovatively developed a universal in situ derivatization workflow combined with desorption electrospray ionization MS/MS (DESI-MS/MS) for mapping endogenous amine-containing metabolites, with trimethylamine (TMA) selected as a case study. For the first time, tert-butyl bromoacetate (TBBA) was used as an in situ derivatization reagent, which improved sensitivity and reproducibility by overcoming the strong matrix effect and signal instability of low molecular weight compounds in MS analysis. Our data suggested that the derivatization efficiency was affected by the alkalinity of the tissue surface, while spray/extraction solvent significantly affected the sensitivity for TMA-TBBA. Notably, DESI-MS/MS exhibited good linearity, reproducibility, and stability in the analysis of amine-containing metabolites derived from TBBA. The optimized workflow was utilized for the visual analysis of endogenous TMA distribution in the brain and serum of mice, revealing the enrichment characteristics of TMA in the cortex and hippocampus, as well as the severe accumulation of TMA in aged animals. Therefore, this targeted DESI-MS/MS-MSI approach, combined with in situ derivatization, offers novel avenues for the routine quantitation of intratissue amine-containing metabolites. In principle, this assay can be extended to a wide variety of metabolites in different biological samples.
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产品关联
DOI
PubMed
高影响因子
BIOMEDICAL CHROMATOGRAPHY
·
2025
·Vol. 39
·期 7
·e70121
·
IF 1.7
Aoli Shang,Yadong Li,Ying Zhao,Jun Chen
This study aimed to investigate the differences in polysaccharides in Astragali Radix (AR) from different sources, as well as before and after honey processing, through the analysis of enzymatic hydrolysates. The hydrolysis product of astragalus polysaccharides (APS) by β-galactosidase was characterized using hydrophilic interaction ultra-high-performance liquid chromatography coupled with time-of-flight mass spectrometry (HILIC-UHPLC-QTOF/MS). The results indicated that all oligosaccharide fragments were composed of hexoses, with sugar groups linked by 1,6-glycosidic bonds. APS enzymatic hydrolysates fingerprints were established using hydrophilic interaction high-performance liquid chromatography with an evaporative light scattering detector (HILIC-HPLC-ELSD) and comprehensively evaluated based on their similarity. Semi-quantitative analysis of each enzymatic hydrolysate was performed, combined with partial least squares discriminant analysis (PLS-DA) and significance testing to further analyze the main differential sugar fragments of AR in different growth modes and before and after honey processing. Glucose and oligosaccharides with degree of polymerization (DP) of 5 and 6 were identified as indicators to distinguish different growth modes. Glucose can serve as an indicator to distinguish raw AR from honey-processed AR (HAR). This study identified quality evaluation indicators of APS based on saccharide mapping, providing a reference for the development of quality standards for AR.
研究领域:
中药学
·
分析化学
·
植物化学
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产品关联
DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
·
2025
·
IF 8.2
Potao Sun,Wenqing Yang,Wenxia Sima,Tao Yuan,Ming Yang,Ninglong Fu,Zhaoping Li,Xiaoxiao Chen
Real-time, accurate, and passive temperature monitoring is critical for industrial and scientific applications. However, conventional temperature sensors often require external power, rely on complex instrumentation, and may perturb the thermal field, compromising measurement accuracy in passive sensing scenarios. Although thermochromic materials offer visual and passive temperature feedback, their utility is limited by narrow sensitivity ranges and subjective interpretation. To address these challenges, this study introduces a machine-vision-enabled microarray passive temperature sensor (MAPTS) inspired by the cooperative perception mechanism of insect compound eyes. The system comprises arrays of organic thermochromic materials patterned via soft lithography on flexible, thermally conductive substrates, enabling wide-range passive thermal sensing. A deep learning-based ResNet-34 architecture deciphers the color-to-temperature relationship from optical images, facilitating high-precision, noncontact regression-based temperature prediction. Experimental results demonstrate that the MAPTS achieves dynamic thermal responses across 0–70 °C with a rapid prediction time of 50 ms. In a high-density 7 × 7 array configuration, the system exhibits better extrapolation performance (R2 = 0.9996) and higher prediction accuracy (mean absolute error ≤ ±0.3 °C), compared to conventional thermochromic sensing methods. This work presents a cost-effective, highly accurate, and reliable approach for intelligent temperature monitoring in diverse applications.
研究领域:
传感器
·
光学传感
·
热工学
·
人工智能
·
材料科学
·
柔性电子
·
工业监测
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ELECTROPHORESIS
·
2016
·
IF 2.482
Shumin Chen,Meiling Wen,Shujie Bu,Ahui Wang,Ya Jin,Wen Tan
Plasma samples from adult male rats were separated by nondenaturing micro 2DE and a reference gel was selected, on which 136 CBB-stained spots were numbered and subjected to in-gel digestion and quantitative LC-MS/MS. The analysis provided the assignment of 1–25 (average eight) non-redundant proteins in each spot and totally 199 proteins were assigned in the 136 spots. About 40% of the proteins were detected in more than one spot and 15% in more than ten spots. We speculate this complexity arose from multiple causes, including protein heterogeneity, overlapping of protein locations and formation of protein complexes. Consequently, such results could not be appropriately presented as a conventional 2DE map, i.e. a list or a gel pattern with one or a few proteins annotated to each spot. Therefore, the LC-MS/MS quantity data was used to reconstruct the gel distribution of each protein and a library containing 199 native protein maps was established for rat plasma. Since proteins that formed a complex would migrate together during the nondenaturing 2DE and thus show similar gel distributions, correlation analysis was attempted for similarity comparison between the maps. The protein pairs showing high correlation coefficients included some well-known complexes, suggesting the promising application of native protein mapping for interaction analysis. With the importance of rat as the most commonly used laboratory animal in biomedical research, we expect this work would facilitate relevant studies by providing not only a reference library of rat plasma protein maps but a means for functional and interaction analysis.
研究领域:
蛋白质组学
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生物化学
·
医学研究
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产品关联
DOI
PubMed
高影响因子
ACTA PHARMACOLOGICA SINICA
·
2021
·Vol. 43
·期 7
·1749-1757
·
IF 6.15
Li Dan,Ding Jin,Liu Te-li,Wang Feng,Meng Xiang-xi,Liu Song,Yang Zhi,Zhu Hua
The spike protein of SARS-CoV-2 interacts with angiotensin-converting enzyme 2 (ACE2) of human respiratory epithelial cells, which leads to infection. Furthermore, low-dose radiation has been found to reduce inflammation and aid the curing of COVID-19. The receptor binding domain (RBD), a recombinant spike protein with a His tag at the C-terminus, binds to ACE2 in human body. We thus constructed a radioiodinated RBD as a molecule-targeted probe to non-invasively explore ACE2 expression in vivo, and to investigate radiotherapy pathway for inhibiting ACE2. RBD was labeled with [ 124 I]NaI using an N-bromosuccinimide (NBS)-mediated method, and 124 I-RBD was obtained after purification with a specific activity of 28.9 GBq/nmol. Its radiochemical purity was (RCP) over 90% in saline for 5 days. The dissociation constant of 124 I-RBD binding to hACE2 was 75.7 nM. The uptake of 124 I-RBD by HeLa ACE+ cells at 2 h was 2.96% ± 0.35%, which could be substantially blocked by an excessive amount of RBD, and drop to 1.71% ± 0.23%. In BALB/c mice, the biodistribution of 124 I-RBD after intravenous injection showed a moderate metabolism rate, and its 24 h-post injection (p.i.) organ distribution was similar to the expression profile in body. Micro-PET imaging of mice after intrapulmonary injection showed high uptake of lung at 1, 4, 24 h p.i.. In conclusion, the experimental results demonstrate the potential of 124 I-RBD as a novel targeted molecular probe for COVID-19. This probe may be used for non-invasive ACE2 mapping in mammals.
研究领域:
放射学
·
分子生物学
·
病毒学
·
核医学
·
传染病学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
·
2022
·Vol. 102
·期 15
·7405-7413
·
IF 4.125
Ting-tiao Pan,Meiting Guo,Ping Lu,Deyu Hu
BACKGROUND Understanding pesticide penetration behavior is important for effective application of pesticides. However, there is a lack of an effective method to monitor pesticide penetration behavior and its changing process. In the present study, a novel surface-enhanced Raman scattering (SERS) mapping method was used for real-time and in situ tracking of the penetration behaviors of thiram and thiram–organosilicon mixture on cabbage leaves. RESULTS The results suggest that thiram has very weak ability to penetrate into cabbage leaves. However, when the thiram–organosilicon mixture was placed on leaf surfaces, a clear thiram signal was detected inside the leaf after 2 h of exposure, a strong signal was observed after 12 h, and the penetration depth of thiram was approximately 200 μm after 48 h. CONCLUSION SERS mapping was demonstrated to be a reliable method for in situ monitoring of organosilicon-induced thiram penetration into cabbage leaf over time. The present study provides a new reference for rationally selecting adjuvants, effectively applying pesticides, and reducing pesticides residue in food. © 2022 Society of Chemical Industry.
研究领域:
农业科学
·
分析化学
·
植物生理学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）