GLYCOCONJUGATE JOURNAL
·
2018
·Vol. 35
·期 4
·411-420
·
IF 2.548
Wang Chengjian,Yang Meifang,Gao Xi,Li Cheng,Zou Zihua,Han Jianli,Huang Linjuan,Wang Zhongfu
Despite the great significance of release and analysis of glycans from glycoproteins, the existing N -glycan release methods are undermined by some limitations and deficiencies. The traditional enzymatic protocols feature high N -glycan release specificity but are generally costly and inefficient for some types of N -glycans. The existing chemical methods require harsh reaction conditions or are accompanied by the remarkable formation of by-products. Herein, we describe a versatile chemical method for the release and analysis of N -glycans from glycoproteins. This method differs from the existing methods as only aqueous ammonia is used to catalyze the N -glycan release reactions. Optimization of reaction conditions was performed using RNase B as a model glycoprotein and the obtained results indicated a highest N -glycan yield in ammonia at 60 °C for 16 h. Comparison of this method with traditional enzymatic protocols and recently reported NaClO methods confirmed the good reliability and efficiency of the novel approach. We also successfully applied this method to some complex biological samples, such as Ginkgo seed protein, fetal bovine serum (FBS) and hen egg white, and demonstrated its great compatibility with various neutral N -glycans, core α-1,3-fucosylated N -glycans and sialylated N -glycans. This method is very simple and cost-effective, enabling convenient analysis and large-scale preparation of released reducing N- glycans from various biological samples for structural and functional glycomics studies.
研究领域:
生物化学
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分子生物学
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分析化学
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DOI
PubMed
高影响因子
C-Journal of Carbon Research
·
2024
·Vol. 10
·期 3
·62
·
IF 3.9
Xuexin Yuan,Qiqi Zhou,Yu Chen,Hai-Jian Yang,Qingqing Jiang,Juncheng Hu,Cun-Yue Guo
In the present work, inexpensive and commercially available diethyltriamine pentaacetic acid (DTPA) was used as an initiator to catalyze the N-formylation reaction of CO2with amines via the construction of C-N bonds in the presence of xanthone as the photosensitizer and PhSiH3as the reducing agent. After a systematic study of various factors, the optimal conditions for the photocatalytic reaction were obtained: 2.5 mmol of amine, 2.5 mmol of PhSiH3, 10 mol% of DTPA, 20 mol% of xanthone, 1 mL of dimethylsulfoxide (DMSO), atmospheric pressure, and 35 W UV lamp irradiation for 48 h. Under the optimal conditions, the catalyst system afforded high performance for the N-formylation of amines (primary and secondary amines) and CO2, and the yields of the N-formylated products of dialkylamines were above 70%. Further studies exhibit that the catalytic system has a wide scope of substrate applications. For various alicyclic secondary amines, heterocyclic secondary amines, aliphatic primary amines, and aromatic primary amines, the corresponding N-formylation products can be obtained efficiently. In addition, the catalyst can be recycled by simple precipitation and filtration. After five cycles of recycling, there was no significant change in the catalytic and structural properties of DTPA. Finally, a possible reaction mechanism is proposed.
研究领域:
化学
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环境科学
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材料科学
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产品关联
DOI
高影响因子
ACS Applied Materials & Interfaces
·
2024
·
IF 8.3
Jing Zhang,Zishuo Fang,Heng Dong,Yu Liu,Zhenyu Chu,Yongbin Mou,Wanqin Jin
In clinical settings, saliva has been established as a straightforward, noninvasive medium for diagnosing periodontitis. However, the precise diagnosis is often hampered by the absence of a specialized analyzer capable of detecting low concentrations of biomarkers typically found in saliva. In this study, we present a noninvasive, on-site screen-printed biomicrochip specifically engineered for the precise and sensitive quantification of lactate concentrations in saliva, a critical biomarker in the diagnosis of periodontitis. The microchip is constructed using a nanostructured ink formulation that includes MnFe@N-doped carbon nanotubes (MnFe@N-CNTs). These MnFe@N-CNTs exhibit a high degree of graphitization and low electrical resistance, significantly augmenting the electrocatalytic efficiency of the enzymatic reaction of lactate. This results in doubled sensitivity and a detection limit that surpasses those of the current advanced salivary assay methods. Remarkably, within just 30 s, the biomicrochip can quantitatively and precisely measure lactate concentrations in the saliva of 10 patients, which provides valuable insights into the severity of their periodontitis. This biosensor holds excellent potential for large-scale production and could broaden the scope of biomarker recognition, paving the way for the analysis of a wider range of oral diseases.
研究领域:
生物医学工程
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临床诊断
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材料科学
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产品关联
DOI
PubMed
高影响因子
Journal of Energy Storage
·
2024
·
IF 8.9
Jing Huang,Jiang Peng,Junqing Zeng,Liping Zheng,Huajie Chen
Despite the important advances on B/N co-doped porous carbons (BNCs), integrating high B/N-doping content and hierarchical porosity into carbon nanosheets remains a challenging task for supercapacitor application. Herein, we report a novel BNC nanosheet with both expected properties through one-step H 3 BO 3 -mediated carbonization/activation of a newly-developed N/O-rich polymer (DPP). In such a mixture system, an in-suit crossing-linked DPP@H 3 BO 3 composite is formed due to the formation of multiple intermolecular hydrogen bonds between the H 3 BO 3 and the ester groups of DPP. The improved thermal stability for such a composite enables the fixation of more B-/N-bearing species in the carbon skeleton during carbonization. Particularly the nanosheet-like BNC-850 shows a rather high B/N content (6.63/7.27 at.%), a large S BET value (766.5 m 2 g −1 ), and a hierarchical porosity, all of which are in favor of electrochemical performance in both aqueous supercapacitors and zinc-ion capacitors (ZICs). In 6 M KOH electrolyte, the BNC-850 electrodes deliver an impressive capacitance of 341.5 F g −1 at 0.5 A g −1 , a high capacitance retention of 79.2 % at 20 A g −1 , and a good cycling stability (95 % capacitance retention over 10,000 cycles at 5 A g −1 ). The energy density of BNC-850-based ZICs is as high as 126.0 Wh kg −1 at 459.0 W kg −1 , exhibiting an outstanding superiority compared to the reported carbon-based ZICs. This work offers a facile strategy for accessing BNCs with high B/N-doping contents, and highlights the importance of such materials in high-energy-density ZICs.
研究领域:
材料科学
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电化学
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能源存储
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产品关联
DOI
高影响因子
Soft Matter
·
2015
·Vol. 11
·期 14
·2885-2892
·
IF 4.029
Dashuang Fan,Peizhu Zheng,Yuanming Ma,Tianxiang Yin,Jihua Zhao,Weiguo Shen
The concentration-dependent enthalpies of mixing for water/sodium bis(2-ethylhexyl)-sulfosuccinate (AOT)/n-alkane microemulsions with different water contents ω0 and chain lengths n of n-alkane were determined by isothermal titration microcalorimetry (ITC) and flow-mixing microcalorimetry at 298.15 K and used to calculate the interaction enthalpies (−ΔHC) between the droplets. It was found that −ΔHC increased with ω0, and changed from negative to positive at about ω0 = 10. The investigation of the dependence of −ΔHC on n revealed that the values of −ΔHC were negative and had a minimum for ω0 = 5; while they were positive and had a maximum for ω0 = 15. These phenomena were discussed based on the competition of the overlapping contribution of the surfactant tails between two neighbouring droplets and the penetration contribution of the solvent molecules into the surfactant tails. These results indicated the important role of entropy in the stability of the microemulsion systems.
研究领域:
化学
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物理化学
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产品关联
DOI
PubMed
高影响因子
FUEL
·
2024
·
IF 6.7
Jing Li,Xiaorui Yan,Yujing Ren,Tiantian Liu,Kairui Li,Haisheng Wei
Design of metal Co-based catalysts by different strategies has aroused many attentions in the chemoselective hydrogenation of nitroarenes to improve their catalytic performance. Herein, Co(6)-N-C catalyst with highly dispersed metal Co and Co-N species was synthesized by the assistance of formed Zn species, which exhibits better performance than single atom Co catalyst in the selective hydrogenation of 3-nitrostyrene, in which the increasing of hydrogenation activity is usually accompanied by unsatisfactory selectivity. In this work, Co(6)-N-C catalyst obtains 98.5 % conversion and > 99 % selectivity of 3-vinylaniline, as well as excellent cyclic stability and desirable substrate universality for the hydrogenation of other substituted nitroarenes. It is demonstrated that the good performance is ascribed to the synergy of metallic Co and Co-N species in the Co(6)-N-C catalyst, improving the activity and selectivity. This work provides a novel strategy to construct an efficient Co-based catalyst for enhanced hydrogenation performance.
研究领域:
催化化学
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材料科学
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产品关联
DOI
高影响因子
JOURNAL OF NANOPARTICLE RESEARCH
·
2014
·Vol. 16
·期 7
·1-12
·
IF 2.278
Zhao Lianhong,Yan Xiufang,Jie Zhiqiang,Yang Hong,Yang Shiping,Liang Jie
Regenerable antimicrobial N -halamine/silica hybrid nanoparticles (NPs) containing chlorinated 5,5-dimethylhydantoinyl (Cl-DMH) groups, Cl-DMH/SiO 2 hybrid NPs, have been prepared by a co-condensation reaction between N -(3-triethoxysilylpropyl)-5,5-dimethylhydantoin (TS-DMH) and tetraethoxysilane (TEOS) and then a chlorination reaction in NaClO solution. The as-synthesized Cl-DMH/SiO 2 NPs were characterized by transmission electron microscopy, Scanning electron microscopy, X-ray photoelectron spectra, Specific surface area, Differential scanning calorimetry, and Fourier transform infrared. Experimental results showed that the size of the as-synthesized Cl-DMH/SiO 2 NPs could be well adjusted by changing the mass ratio of TS-DMH/TEOS and the volume ratio of 28 % NH 4 OH/H 2 O. Antimicrobial tests showed that the as-prepared Cl-DMH/SiO 2 hybrid NPs had excellent antimicrobial activities against both Escherichia coli and Staphylococcus aureus . The minimum inhibitory concentration and minimum bactericidal concentration values of the as-prepared Cl-DMH/SiO 2 hybrid NPs are 15 and 20 μg/mL for S. aureus , 25 and 30 μg/mL for E. coli , respectively. Paper disk diffusion assay showed that smaller-sized Cl-DMH/SiO 2 hybrid NPs have bigger inhibition zone diameters, indicating stronger antimicrobial efficacies. Also, the storage stability and regenerability of Cl-DMH/SiO 2 hybrid NPs were investigated.
研究领域:
材料科学
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微生物学
·
化学工程
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产品关联
DOI
高影响因子
JOURNAL OF CHEMICAL THERMODYNAMICS
·
2022
·
IF 3.269
Pan Zhang,Yanxi Ji,Wenya Li,Lihua Xu,Lemeng Wang,Dong Fu
The viscosities of N -(2-aminoethyl)ethanolamine (AEEA), 1,4-butanediamine (BDA) and N-methyl- 1,3-propane- diamine (MAPA) activated 2- diethylaminoethanol (DEAE) aqueous solutions were measured at temperatures ( T ) from 303.2 K to 323.2 K, respectively. The DEAE mass fractions ( w DEAE ) and the other three amines ( w AEEA / w BDA / w MAPA ) are 0.300 to 0.500 and 0.050 to 0.150 respectively. The Weiland equation was used to calculate the viscosity of these three solutions, and the calculation results were satisfied with data of the experimental results. With the aid of experimentally determined viscosity data, the activation energy ( E a) of these three solutions was predicted through the Arrhenius formula. With the help of the viscosity data and the diffusion coefficient model of CO 2 in water, the CO 2 diffusion coefficient ( D CO2 ) in these three solutions was calculated. The quantitative relationship between the calculated values (viscosity, activation energy and diffusion coefficient) and the operation conditions (solution mass fraction and temperature) was analyzed. Our results show that the DEAE-BDA absorbent has better application potential due to its lower viscosity, higher CO 2 diffusion coefficient and lower activation energy.
研究领域:
化学工程
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环境科学
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DOI
高影响因子
VACUUM
·
2024
·
IF 3.8
Xiaoying Yu,Chunhong Gao,Jinjia Liu,Jiang Wang,Meilin Jia,Gala Sa,Aiju Xu
Photocatalytic technology has good stability and ecological friendliness which is used to treat organic pollutants in wastewater. In this paper, TiO 2 , 1.5%V–TiO 2 and N, V co-doped TiO 2 were synthesized by hydrothermal method for photocatalytic degradation of tetracycline. Meanwhile, XRD , HRTEM , SEM, FT-IR, XPS, UV–Vis DRS , PL and electrochemistry tests were used to characterize the catalysts. The results showed that the appropriate amount of N, V co-doped can cause structural changes of TiO 2 . The co-doped of N and V enhanced the photocurrent response of TiO 2 , increased the specific surface area, reduced the band gap, and improved the efficiency of photodegradation of tetracycline. Accordingly, the tetracycline photocatalytic degradation path was proposed. 6%N-1.5%V–TiO 2 completely degraded tetracycline into CO 2 , H 2 O and NO 3 − . The calculation results show that an appropriate amount of N, V co-doped TiO 2 decreased bandgap, improved the optical absorption performance of TiO 2 . This work demonstrated the potential of N, V co-doped to design efficient photocatalysts .
研究领域:
环境科学
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材料科学
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产品关联
DOI
高影响因子
CHEMICAL PHYSICS LETTERS
·
2024
·
IF 2.8
Ting-ting Yan,Yi-fei Xu,Dong-yang Xi,Zhiqing Yu,Long Ma,Dan-dan Zhang,Ran Jiang
The study investigated the behavior of N-nitropyrazole (1-NP, C 3 H 3 N 3 O 2 ) under high pressure using a diamond anvil cell, Raman spectroscopy, and simulation calculations. The pressure–volume relationship of the sample, molecular interactions, and changes in optical Raman were analyzed. The Raman experiments revealed abnormal vibration intensities and a discontinuity in peak position at 2.2 GPa. For instance, the C-H stretching vibration produces a new vibration peak under pressure, and the NO 2 torsion mode continues to red shift beyond 2.2 GPa. These observations suggest that the crystal’s phase transition results from the distortion of its hydrogen-bonded network structure. The pressure relief experiment confirms the reversibility of the phase transition.
研究领域:
化学
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材料科学
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DOI
高影响因子
CHIRALITY
·
2024
·Vol. 36
·期 2
·e23643
·
IF 2.8
Xiaojun Li,Qi Li,Liying Yang,Liqin Huang,Chenchen Peng,Jianyong Zheng
In this study, lipase-catalyzed resolution of N-acetyl-DL-methionine methyl ester (N-Ac-DL-MetOMe) was evaluated. A lipase from Brucella thiophenivorans was prone to exhibit high activity and excellent enantioselectivity toward N-Ac-DL-MetOMe to produce the key chiral intermediate N-acetyl-L-methionine methyl ester (N-Ac-L-MetOMe). The results showed that the enzymatic reaction was carried out in 100 g/L racemic substrate for 2 h, the conversion reached 51.3%, the enantiomeric excess value N-Ac-L-MetOMe exceeded 99%, and the enantiomeric ratio value >200. Therefore, the lipase from B. thiophenivorans has potential prospects for the resolution of N-Ac-DL-MetOMe to produce the important intermediate N-Ac-L-MetOMe.
研究领域:
生物催化
·
酶工程
·
有机化学
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DOI
PubMed
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2013
·Vol. 37
·期 8
·2537-2540
·
IF 2.966
Songbai Liu,Jiahui Zhou
Surfactants have long been underestimated in synthetic organic chemistry despite their great potential in promoting solubility, reactivity and selectivity in metal-mediated cross-couplings. In this study the strategy of surfactant promotion was exemplified in copper-catalyzed N-arylation of indoles in water. The different effects of surfactants during the coupling reaction were first explored. The superior promoting effect of the natural zwitterionic surfactant, betaine, was found and attributed to the capability of the formed complex to stabilize the copper intermediate in the catalytic transformation and facilitate transportation of the reactants. The concept of surfactant promotion through complexation is valuable in metal-mediated cross-couplings. This method also exhibited good recycling ability. This study will greatly facilitate exploration of surfactant promotion in metal-mediated cross-couplings.
研究领域:
有机化学
·
合成化学
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DOI
高影响因子
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