COLLOIDS AND SURFACES B-BIOINTERFACES
·
2024
·
IF 5.4
Quanshi Lin,Yichao Wang,Linlin Wang,Zhijin Fan
Significant progress has been made in cancer immunotherapy; however, challenges such as interpatient variability, limited treatment response, and severe side effects persist. Although nanoimmunotherapy has emerged as a promising approach, the construction of precise and efficient nanosystems remain formidable challenges. Herein, a multifunctional nanoplatform was developed using macrophage-derived cellular vesicles (MCVs) for NIR-II imaging-guided precise cancer photo-immunotherapy. MCVs exhibited excellent tumor targeting and TAMs re-education effects, serving as both delivery carriers and therapeutic agents. Through amide bond, indocyanine green (ICG) was conjugated to the surface of MCVs, enabling in vivo tracking of MCVs distribution. Notably, ICG exhibited dual functionality as a NIR-II fluorescent agent and possessed photodynamic and photothermal effects, enabling the conversion of light energy into chemical or heat energy to eliminate tumor cells. This precision phototherapy triggered immunogenic cell death (ICD) of tumor, thereby activating the anti-tumor immune response. Additionally, MCVs loaded with R848, a toll-like receptor agonist, augmented the ICD-induced anti-tumor immunity. Animal experiments confirmed that MCVs-mediated photoimmunotherapy promoted T cell infiltration, inhibited tumor growth, and improved survival rates. In conclusion, we have developed a promising precision immunotherapy strategy capable of enhancing the immune response while mitigating off-target effects. These findings offer encouraging prospects for clinical translation.
研究领域:
癌症免疫治疗
·
纳米技术
·
生物医学工程
·
免疫学
·
药理学
✓ 已验证
DOI
PubMed
高影响因子
Advanced Healthcare Materials
·
2024
·
IF 10
Yanbin Zhao,Yangyang Li,Bin Wang,Junyan Yao,Yue Fan,Peng He,Jing Bai,Cheng Wang,Feng Xue,Chenglin Chu
Magnesium phosphate bone cement (MPC) has gained widespread usage in orthopedic implantation due to its fast-setting and high initial strength benefits. However, the simultaneous attainment of drug-controlled release and osteogenic potential in MPC remains a significant challenge. Herein, a strategy to create a smart injectable cement system using nanocontainers and chondroitin sulfate is proposed. It employs nanocontainers containing alendronate-loaded mesoporous silica nanoparticles, which are surface-modified with polypyrrole to control drug release in response to near-infrared (NIR) stimulation. The alendronate-incorporated cement (ACMPC) exhibits improved compressive strength (70.6 ± 5.9 MPa), prolonged setting time (913 s), and exceptional injectability (96.5% of injection rate and 242 s of injection time). It also shows the capability to prevent degradation, thus preserving mechanical properties. Under NIR irradiation, the cement shows good antibacterial properties due to the combined impact of hyperthermia, reactive oxygen species, and alendronate. Furthermore, the ACMPC (NIR) group displays good biocompatibility and osteogenesis capabilities, which also lead to an increase in alkaline phosphatase activity, extracellular matrix mineralization, and the upregulation of osteogenic genes. This research has significant implications for developing multifunctional biomaterials and clinical applications.
研究领域:
生物材料
·
骨科
·
药物递送系统
·
组织工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2023
·
IF 15.1
Shibo Xu,Yiyi Lin,Xingjun Zhao,Zepeng Liang,Yanan Hu,Zhenhua Chen,Xiuli Ren,Xifan Mei
Osteoarthritis (OA) is one of the leading causes of chronic disability in the elderly. Effective clinical interventions for OA are still lacking, and new therapeutic measures are urgently needed. The emerging near-infrared light (NIR) sensitive nanozymes are attractive for their great potential and appeal in treating OA by repairing mitochondrial function and improving impaired autophagic flux. In this study, a NIR-sensitive tea polyphenol-modified zirconium-based porphyrin metal–organic framework ( [email protected] ) was introduced as a multifunctional nanoplatform for the treatment of OA. TEM and EDS mapping revealed that gold nanoclusters were uniformly distributed on the surface of the spherical particles. Electron spin resonance (ESR) analysis revealed that nanozymes could alleviate different types of oxidative stress. Molecular biology indicated that [email protected] reduced chondrocyte apoptosis and increased chondrocyte metabolism by 80.7%. An increase in chondrocyte-expressed type II collagen and proteoglycan content ensues. Injection of [email protected] effectively raised the joint temperature to 46.9 °C and controlled drug delivery under NIR excitation. Section staining revealed that eight weeks of intervention decreased the OARSI score in OA rats by approximately 67.0%. Transmission electron microscopy confirmed a significant improvement in intracellular mitochondrial morphology. The present study provides evidence that modifiable multifunctional nanozymes effectively repair cartilage-damaged mitochondria and promote cartilage regeneration, representing an appealing therapeutic strategy for OA.
研究领域:
纳米技术
·
生物医学工程
·
材料科学
·
骨科
·
药理学
✓ 已验证
产品关联
DOI
高影响因子
ACS Nano
·
2023
·
IF 17.1
Weilun Pan,Bodeng Wu,Chengtao Nie,Tingting Luo,Zhenli Song,Jie Lv,Yong Tan,Chunchen Liu,Mingzhen Zhong,Tong Liao,Zhenxun Wang,Guanghui Yi,Limin Zhang,Xiaoliu Liu,Bo Li,Jinxiang Chen,Lei Zheng
Developing an effective dressing against bacterial infection and synchronously addressing wound complications, such as bleeding, long-term inflammation, and reinfection, are highly desirable in clinical practice. In this work, a second near-infrared (NIR-II) responsive nanohybrid consisting of imipenem encapsulated liposome with gold-shell and lipopolysaccharide (LPS)-targeting aptamer, namely ILGA, is constructed for bacteria elimination. Benefiting from the delicate structure, ILGA exhibits strong affinity and a reliable photothermal/antibiotic therapeutic effect toward multidrug-resistant Pseudomonas aeruginosa (MDR-PA). Furthermore, by incorporating ILGA with a thermosensitive hydrogel poly(lactic-co-glycolic acid)–polyethylene glycol–poly(lactic-co-glycolic acid) (PLGA-PEG-PLGA), a sprayable dressing ILGA@Gel was prepared, which enables a quick on-demand gelation (10 s) for wound hemostasis and offers excellent photothermal/antibiotic efficacy to sterilize the infected wound. Additionally, ILGA@Gel provides satisfactory wound-healing environments by reeducating wound-associated macrophages for inflammation alleviation and forming a gel layer to block exogenous bacterial reinfection. This biomimetic hydrogel reveals excellent bacteria eradication and wound recovery effectiveness, demonstrating its promising potential for managing complicated infected wounds.
研究领域:
生物医学工程
·
纳米技术
·
药学
·
伤口愈合
·
传染病
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2021
·
IF 8.128
Huaying Hou,Zhongxu Wang,Yajie Ma,Kai Yu,Jingxiang Zhao,Huiming Lin,Fengyu Qu
The intracellular O 2 -supply not only can relieve tumor hypoxia but also enhance the effects of photodynamic therapy (PDT). In this work, metallic Mo 2 [email protected] N [email protected] nanoparticles were constructed to reveal the near infrared (NIR)-photocatalytic O 2 generation and promote photodynamic therapy (PDT). Here, (NH 4 ) 6 Mo 7 O 24 ·4H 2 O nanorods and urea were adopted as resources that were calcined to obtain Mo 2 [email protected] N -carbon nanoparticles (20 nm). All samples displayed high NIR absorption as well as photothermal conversion efficiency of up to 52.7 % (Mo 2 [email protected] N [email protected] ). The density functional theory calculations demonstrated the metallic characteristic of Mo 2 C and that the consecutive interband/intraband charge-transition was responsible for the high NIR harvest and redox ability of electron-hole pairs, making the NIR-photocatalytic O 2 and reactive oxygen species (ROS) generation. In comparison with the pure Mo 2 C, the heterostructure displayed twice the performance due to the enhanced charge-segregation between Mo 2 C and N -carbon. Given the high X-ray absorption coefficient and photothermal ability, the nanocomposite could be used in novel computer tomography and photothermal imaging contrast. Furthermore, the novel biodegradation and metabolism behaviors of nanocomposites were investigated, which were reflected as elimination from the body (mouse) via feces and urine within 14 days. The as-synthesized Mo 2 [email protected] N [email protected] nanocomposites integrated the dual-model imaging, intracellular O 2 -supply, and phototherapy into one nanoplatform, revealing its potential for anti-cancer therapy.
研究领域:
生物医学工程
·
材料科学
·
纳米技术
·
癌症治疗
·
成像技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOMATERIALS
·
2020
·
IF 10.317
Lei Huang,Wenxi Yan,Bo Cai,Yu Song,Qiying Lv,Guobin Wang,Lin Wang,Zheng Wang
To engineer tumor-tropic cells as drug delivery vehicles is a promising strategy to improve therapeutic specificity and efficacy for cancer treatment. However, conventional genetically engineered cell-based drug delivery systems are often capable of initiating single-mode therapy, and lack precise spatiotemporal control over the release of therapeutic payloads at tumor local, thus possibly causing severe systemic toxicity. Here, the macrophages are genetically engineered to encode a non-secreted form of EGFP-TNFα fusion protein and intracellularly carry near-infrared (NIR)-responsive heat-nanogenerators (HIMs). Owing to macrophages' intrinsic tumor tropism and HIMs’ photo-responsiveness to NIR, these macrophages ( [email protected] ET ) can actively accumulate at tumor sites and undergo controlled photothermolysis induced by NIR-induced HIMs-mediated photothermal effects (PTE). Such heat-induced cell explosion enables spatiotemporally controlled release of non-secreted TNFα from macrophages and effectively kills cancer cells. Importantly, in a preclinical tumor model, [email protected] ET actively migrate to tumors where PTE and released EGFP-TNFα exhibit an enhanced antitumor effect, suppressing tumor growth and significantly prolonging animal survival without eliciting adverse side effects. Thus, this study demonstrates the potential of such dual-engineered macrophages in bi-modal cancer therapy.
研究领域:
癌症治疗
·
生物医学工程
·
药理学
·
细胞生物学
·
纳米技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Biomaterials Science
·
2019
·Vol. 8
·期 2
·720-738
·
IF 5.251
Siming Yu,Guanning Huang,Riming Yuan,Tianfeng Chen
In the present work, an iridium (Ir) complex loaded theranostic nanoplatform was designed for high-efficiency triple-negative breast cancer (TNBC) therapy. For this purpose, the Ir complex was firstly loaded on a photothermal agent of gold nanostars (GNS) by simply mixing followed by functionalization using a urokinase-type plasminogen activator receptor (uPAR) targeted polyetherimide-AE105 peptide conjugate (P-AE105) with the formation of GNS@Ir@P-AE105. It was demonstrated that the resultant GNS@Ir@P-AE105 was a multifunctional nanoplatform with advantages of (1) NIR laser controlled release of the Ir complex; (2) precise delivery of the Ir complex to TNBC cells; (3) excellent photothermal (PT)/photoacoustic (PA)/X-ray computed tomography (CT) tri-modal imaging ability; and (4) a synergistic photothermal-chemotherapeutic effect. An in-depth investigation of the mechanism revealed that binding forces of the Ir complex–GNS and P-AE105–GNS were significantly diminished upon NIR laser irradiation, which conferred an NIR laser-responsive Ir complex release property to the nanoplatform. Moreover, the nanoplatform exerted high efficiency anti-TNBC effects via a ROS-induced p53 apoptotic pathway. Specifically, combinational photothermal-chemotherapeutic treatments stimulated intracellular ROS generation, which significantly up-regulated apoptotic-relative p53 gene expression either by causing severe DNA damage or inducing an arrest effect on the sub-G1 phase of the cell cycle. Taken together, our work provides a novel theranostic nanoplatform for efficient and simultaneous diagnosis and therapy of TNBC.
研究领域:
癌症治疗
·
纳米医学
·
医学影像
·
生物医学工程
·
肿瘤学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Yiyu Wang,Chunqing Niu,Guiting Yu,Yuhong Lin,Binbin Li,Zheng Jin,Xiaona Wu,Jian Shi,Chibo Liu,Xinyu Wang,Kai Zhao
Angiogenesis plays a vital role in the treatment of full-thickness wounds. Deferoxamine (DFO) has been employed to promote neovascularization, however, smart drug delivery systems are needed to optimize its utilization. In this study, an injectable extracellular matrix (ECM)-mimicking hydrogel (HOG@P&D) was developed by leveraging the dynamic Schiff base and hydrogen bonds among a chitosan derivative (HACC), oxidized alginate (OSA), gelatin, and DFO-loaded polydopamine nanoparticles (P&D) for efficient wound healing. The incorporation of P&D enables HOG@P&D to respond to near-infrared (NIR) irradiation, converting laser energy into heat to trigger an on-demand, rapid release of DFO, thereby effectively enhancing angiogenesis. In vitro tube formation assays revealed that the number of meshes in the HOG@P&D group was fourfold higher than that of the control group. Additionally, HOG@P&D exhibited superior mechanical properties, tissue adhesion, and injectability, allowing it to cover wounds seamlessly. This hydrogel also demonstrated antibacterial and antioxidant properties, creating a conducive microenvironment for wound healing. In vivo studies further confirmed that HOG@P&D promoted angiogenesis and mitigated inflammation by upregulating angiogenic growth factor expression, thereby accelerating full-thickness wound healing. This nanocomposite hydrogel shows significant potential as a high-performance wound dressing.
研究领域:
生物医学工程
·
组织工程
·
伤口愈合
·
药物递送系统
·
纳米技术
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Bioactive Materials
·
2024
·
IF 18
Tian Zhang,Dongsheng Tang,Pengfei Wu,Shaoping Jiang,Yuquan Zhang,Abid Naeem,Yong Li,Chunhui Li,Bo Hu,Shuai Guo,Caixia Sun,Haihua Xiao,Ran Yan,Yuhua Weng,Yuanyu Huang
Immune checkpoint blockade (ICB) therapy is a widely favored anti-tumor treatment, but it shows limited response to non-immunogenic “cold” tumors and suffers from drug resistance. Photodynamic therapy (PDT), as a powerful localized treatment approach, can convert a “cold tumor” into a “hot tumor” by inducing immunogenic cell death (ICD) in tumor cells, thereby enhancing tumor immunogenicity and promoting tumor immunotherapy. However, the effectiveness of PDT is largely hindered by the limited penetration depth into tumor tissues. To address these issues, we proposed an all-in-one drug system with NIR-II photo-accelerated PDT effects, efficient immune checkpoint gene silencing, and a facile manufacturing process. The so-called all-in-one drug system comprises a multi-modal designed polymer PPNP and siRNA. PPNP is an amphipathic polymer that includes the near infrared-II (NIR-II) photosensitizer Aza-boron-dipyrromethene (Aza-BODIPY), a glutathione (GSH)-cleavable linker, and a cationic monomer derived from cholesterol. PPNP can self-assemble and efficiently load siRNA. Under laser irradiation, PPNP triggers a potent ICD cascade, causing the on-demand release of siPD-L1, reshaping the tumor's immunosuppressive microenvironment, effectively inhibiting the growth of various tumors, and stimulating the immune memory. This study represents a generalized platform for PDT and gene silencing, designed to modulate immune-related signaling pathways for improved anticancer therapy.
研究领域:
肿瘤学
·
免疫治疗
·
药理学
·
生物医学工程
·
纳米技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CERAMICS INTERNATIONAL
·
2021
·
IF 4.527
Beibei Zhu,Jun Shi,Chongchong Liu,Jingguo Li,Shaokui Cao
As a novel two-dimensional material, Ti 3 C 2 MXenes has attracted lots of attention in biomedical filed for its large surface area and excellent near-infrared (NIR) responsiveness. In this paper, an in-situ growth and self-assembly approach was employed to combine gold nanorods (GNRs) with Ti 3 C 2 nanosheets to prepare intelligent sandwich-like Ti 3 C 2 @GNRs/PDA/Ti 3 C 2 nanohybrids . Compared with Ti 3 C 2 nanosheets, in-situ growth Ti 3 C 2 @GNRs possessed excellent photothermal conversion efficiency (45.89%), caused by the distinguished photothermal synergy between GNRs and Ti 3 C 2 nanosheets. Moreover, the high specific surface area of Ti 3 C 2 MXene and outstanding adhesion performance of PDA endowed Ti 3 C 2 @GNRs/PDA/Ti 3 C 2 nanohybrids with superior drug loading ability for doxorubicin hydrochloride (DOX) (95.88%). Besides, Ti 3 C 2 @GNRs/PDA/Ti 3 C 2 nanohybrids displayed distinct pH/NIR responsive drug release properties upon NIR irradiation owing to the strong π-π stacking interaction between Ti 3 C 2 @GNRs/PDA/Ti 3 C 2 and DOX, along with the excellent NIR-responsiveness of Ti 3 C 2 @GNRs/PDA/Ti 3 C 2 . This paper offers a practicable method to prepare Ti 3 C 2 MXene-based nanoplatform with synergistically enhanced NIR drug release behavior, brilliant biocompatibility and high drug loading efficiency, which is expected to be applied in remote cancer therapy.
研究领域:
生物医学工程
·
纳米技术
·
药学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
DALTON TRANSACTIONS
·
2023
·Vol. 52
·期 19
·6569-6577
·
IF 4
Shihao Wu,Heng Liang,Yan Li,Wanfang Zou,Zhijian Liao,Wei Wang,Xinyu Ye
The Cr3+-doped near-infrared (NIR) phosphors have been extensively investigated owing to their promising applications in biomedicine, food safety detection, and night vision surveillance. However, achieving broadband (FWHM > 160 nm) NIR emission is still challenging. In this paper, novel Y2Mg2Ga2−xSi2O12:xCr3+ (YMGS:xCr3+, x = 0.005–0.08) phosphors were prepared by a high-temperature solid-state reaction. The crystal structure, photoluminescence properties of the phosphor, and the device performance of a pc-LED were researched in detail. When excited at 440 nm, the YMGS:0.04Cr3+ phosphor exhibited broadband emission in the range of 650–1000 nm, peaking at 790 nm with a full width at half-maximum (FWHM) of up to 180 nm. The wide FWHM of YMGS:Cr3+ is conducive to its extensive application in NIR spectroscopic technology. In addition, the YMGS:0.04Cr3+ phosphor could maintain 70% of the initial emission intensity at 373 K. By combining the commercial blue chip with the YMGS:0.04Cr3+ phosphor, the resulting NIR pc-LED demonstrated a near-infrared output power of 14 mW with a photoelectric conversion efficiency of 5% at a drive current of 100 mA. This work provides a broadband emission NIR phosphor option for NIR pc-LED devices.
研究领域:
材料科学
·
光学工程
·
生物医学工程
·
光电子学
·
固体化学
✓ 已验证
DOI
高影响因子
Journal of Materials Chemistry B
·
2020
·Vol. 8
·期 47
·10854-10866
·
IF 5.344
Cuifu Fang,Zhiwei Zhong,Teng Zhang,Shuang Jia,Xingwei Ding,Weimin Zhou,Xiaolei Wang
Problems such as massive hemorrhage caused by uncontrolled drug dosage are the main significant obstacles in clinical thrombolytic therapy, which are prominently due to the lack of targeting and controlled release ability of efficient thrombolytic drug systems. In recent years, our team demonstrated that the photothermal effect can facilitate the thrombolytic effect of urokinase plasminogen activator (uPA). However, conventional photothermal agents are relatively expensive or contain heavy metals. If drug delivery systems with low toxicity, minimized heavy metal elements and easy accessibility (preferably provided by human self) can be developed, they will be of value in the future related applications. Herein, uPA-loaded human black hair derived nanoparticles with gelatin capsules (uPA@HBHNP@GNCs) were applied for the first time as a thrombolytic system. Upon irradiation by near-infrared I window (NIR-I) laser or II window (NIR-II) laser, the photothermal effect of HBHNP was triggered to promote the melting of the gelatin encapsulated around the outer layer, thereby realizing the targeted release of uPA. The in vitro and in vivo experiments demonstrated that the deep response to NIR (especially II window) of this system exhibited a satisfactory thrombolytic effect with ideal biosafety. Briefly, the proposed hair derived drug delivery system has the characteristics of human source, low cost, minimum heavy metal components, deep response to NIR (II window) laser, and good biocompatibility, which is expected to be expanded to the treatment for some diseases, even in deep tissue areas.
研究领域:
药学
·
生物医学工程
·
治疗学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）