SENSORS AND ACTUATORS B-CHEMICAL
·
2025
·
IF 7.7
Huanhuan Li,Yu Wu,Xorlali Nunekpeku,Yuhan Yang,Jingsa Zhao,Wei Sheng,Yuexia Wang
The increasing presence of Aflatoxin B 1 (AFB 1 ) residues in food continues to raise significant public health concerns. Herein, we designed an upconversion-based sensor integrated into an alginate-polyacrylamide (SA-PAM) hydrogel for on-site AFB 1 detection in food. First, core-shell upconversion nanoparticles (CSUCNPs) with enhanced fluorescence were modified with complementary DNA (cDNA), forming signal probes known as CSUCNPs-cDNA. Simultaneously, NH 2 -ZIF-8@Cu 2+ with a high surface area and specific affinity for aptamer was synthesized using a one-pot method. This was then modified with an AFB 1 -specific aptamer, forming the capture probe Apt-ZIF-8@Cu 2+ . Using base pairing, CSUCNPs-cDNA and Apt-ZIF-8@Cu 2+ were hybridized into sensing probes (CSUCNPs-ZIF-8@Cu 2+ ), which were embedded into SA-PAM hydrogels as stable sensor carrier. Furthermore, the SA-PAM hydrogel showed a reduced swelling ratio of 1.68–1.80 in water and phosphate buffer solution(s) (PBS), which improves stability. In the presence of AFB 1 , the aptamer selectively binds the AFB 1 , increasing electrostatic repulsion between CSUCNPs-cDNA and Apt-ZIF-8@Cu 2+ . This disrupts Förster resonance energy transfer (FRET), resulting in a pronounced “signal-on” fluorescence. This sensor achieves AFB 1 diffusion through the SA-PAM hydrogel, FRET disruption and upconversion signal stabilization within 18 min, faster than traditional methods. Under optimized conditions, the sensor exhibited a clear linear correlation between fluorescence intensity and the logarithm of AFB 1 concentration ranging from 0.1 to 100 µg/kg ( R ² = 0.994), with a detection limit as low as 0.08 µg/kg. Besides, its performance was further validated through tests on spiked wheat, corn, peanut, and water, demonstrating high accuracy and reproducibility with relative standard deviation (RSD) below 3.63 % and recoveries of 85.88–98.04 %.
研究领域:
食品安全
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分析化学
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纳米技术
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生物传感器
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毒素检测
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DOI
高影响因子
ACS Applied Materials & Interfaces
·
2024
·
IF 8.3
Derrick Boateng,Xukai Li,Weiyao Wu,Anqi Yang,Anadil Gul,Yan Kang,Lin Yang,Jifang Liu,Hongbo Zeng,Hao Zhang,Linbo Han
Accurate air-writing recognition is pivotal for advancing state-of-the-art text recognizers, encryption tools, and biometric technologies. However, most existing air-writing recognition systems rely on image-based sensors to track hand and finger motion trajectories. Additionally, users’ writing is often guided by delimiters and imaginary axes which restrict natural writing movements. Consequently, recognition accuracy falls short of optimal levels, hindering performance and usability for practical applications. Herein, we have developed an approach utilizing a one-dimensional convolutional neural network (1D-CNN) algorithm coupled with an ionic conductive flexible strain sensor based on a sodium chloride/sodium alginate/polyacrylamide (NaCl/SA/PAM) dual-network hydrogel for intelligent and accurate air-writing recognition. Taking advantage of the excellent characteristics of the hydrogel sensor, such as high stretchability, good tensile strength, high conductivity, strong adhesion, and high strain sensitivity, alongside the enhanced analytical ability of the 1D-CNN machine learning (ML) algorithm, we achieved a recognition accuracy of ∼96.3% for in-air handwritten characters of the English alphabets. Furthermore, comparative analysis against state-of-the-art methods, such as the widely used residual neural network (ResNet) algorithm, demonstrates the competitive performance of our integrated air-writing recognition system. The developed air-writing recognition system shows significant potential in advancing innovative systems for air-writing recognition and paving the way for exciting developments in human-machine interface (HMI) applications.
研究领域:
电气工程
·
机器学习
·
材料科学
·
人机交互
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DOI
PubMed
高影响因子
Journal of Environmental Chemical Engineering
·
2025
·
IF 7.2
Yong Liu,Xiaolu Sun,Linhua Jiang,Hao Jin,Guangbin Zhu,Weidong Li,Shibing Cai,Guitian Shi,Lijun Wang,Haofan Wang,Dekang Yuan,Ning Duan
Sulfidation is recognized as a competitive method to remove arsenic from copper smelting waste acid. However, despite its ability to shed As(Ⅲ) efficiently, the sulfidation driven by S(-Ⅱ) supersaturation levels generates fine particles that severely hamper subsequent solid-liquid separation. In this study, the results manifest that expanding S(-Ⅱ) dosage and downgrading acidity (i.e., H 2 SO 4 concentration) can foster As(Ⅲ) removal, but diminish the particle size distribution of As(Ⅲ) sulfides. Based on the diffusion-growth model, except for in-situ CuS and CdS, concomitant anions and cations decelerate As(Ⅲ) sulfide settling. This is attributed to enhanced negative charges on the particle surface. In pursuit of better settling performance of As(Ⅲ) precipitate, a strengthening strategy of As 2 S 3 crystal seed and polyacrylamide(PAM) flocculant is proposed, where the preparation of As 2 S 3 crystal seed from arsenic waste complies with the green recycling-production standards. By involving 10 g/L As 2 S 3 crystal seed as heterogeneous nucleation and 5 ml/L PAM flocculant as framework support, the settling velocity of As(Ⅲ) sulfide is boosted by approximately 170%, delivering valuable guidance for reinforcing gravity settling performance in thickener processes.
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产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2025
·
IF 7.7
Zhengfang Liang,Shuwei Xiao,Junhong Li,Guoli Zuo,Jihua Wei,Yuanxun Gong,Qianli Tang,Kai Zhang,Xianjiu Liao
Apurinic/apyrimidinic endonuclease 1 (APE1), a key enzyme in the base excision repair pathway, is frequently overexpressed in multiple cancers and has emerged as an important biomarker for early diagnosis and therapeutic response evaluation. In this work, a highly sensitive electrochemiluminescence (ECL) biosensor was developed for APE1 detection by integrating a PAM-regulated CRISPR-Cas12a activation mechanism with a multifunctional nanocomposite-modified electrode. A Ti₃C₂/AuNPs/CsPbBr₃ ternary nanocomposite was fabricated through ultrasonic assembly to provide enhanced conductivity, water stability, and probe-loading capacity. Meanwhile, a DNA duplex containing an AP site within the PAM region was engineered to regulate Cas12a activation through APE1-mediated cleavage and polymerase-assisted repair, thereby minimizing background interference and enabling highly specific signal generation. Ferrocene-labeled DNA tetrahedrons (TDNs) were further introduced as a spatially ordered probe scaffold to improve hybridization efficiency and facilitate Cas12a trans-cleavage–mediated signal release. This work presents three key innovations: (1) Construction of a Ti₃C₂/AuNPs/CsPbBr₃ nanocomposite that simultaneously enhances ECL stability, conductivity, and electron-transfer efficiency, addressing the inherent water-instability of perovskite emitters. (2) Coupling of a PAM-regulated APE1–KF repair mechanism with Cas12a activation to achieve highly specific AP-site recognition and selective signal generation. (3) Introduction of a TDN-based probe architecture that provides rigid spatial orientation and high-density probe loading, markedly improving hybridization efficiency and Cas12a trans-cleavage performance. Benefiting from these synergistic innovations, the biosensor achieves a detection limit of 6.28 × 10⁻⁸ U μL⁻¹ and a wide linear range (1 × 10⁻⁷ to 1 × 10⁻ 1 U μL⁻¹). The platform also demonstrates excellent specificity, reproducibility, and reliable performance in diluted serum, highlighting its promise for clinical and biochemical applications.
研究领域:
生物传感器
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纳米技术
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癌症诊断
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电分析化学
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分子生物学
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CRISPR技术
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产品关联
DOI
高影响因子
Chemical Engineering Journal Advances
·
2026
·
IF 6.9
Wenyao Li,Xinling Wang,Na Xu,Wenjie Yang,Yushi Chen,Shengnan Luo,Fang Ma,Huiping Chen,Zisen Zhang,Liguo Zhang
The development of chromatographic media with uniform particle size, high mechanical strength, and specific binding capacity is crucial for efficient protein purification. However, conventional methods often suffer from polydisperse particle sizes and low magnetic content, limiting their separation efficiency and scalability. Here, we report a scalable strategy integrating high-aspect-ratio (HAR) multi-channel droplet microfluidics with a robust physicochemical modification approach to fabricate monodisperse polyacrylamide-polyethylene glycol (PAM-PEG) magnetic microspheres. The optimized HAR microfluidic chip enabled high-throughput production of droplets with a coefficient of variation (CV) as low as 3.98%. Subsequent incorporation of PEG-modified Fe₃O₄ nanoparticles endowed the microspheres with superparamagnetic properties (saturation magnetization: 40.9 emu/g) and enhanced mechanical resilience. Through a sequential surface functionalization process involving alkaline hydrolysis, EDC/NHS activation, nitrilotriacetic acid (NTA) grafting, and Ni²⁺ chelation, the resulting PAM-PEG@Fe₃O₄-PEG@NTA-Ni²⁺ microspheres achieved a high nickel loading capacity of 50.17 μmol/mL. These microspheres exhibited a specific protein adsorption capacity of ∼44 mg/mL and demonstrated superior selectivity in capturing His-tagged proteins from complex biological matrices, outperforming commercial counterparts in both purity and non-specific binding reduction. This work presents a promising platform for next-generation bioseparation media with high throughput and tailored functionality.
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DOI
高影响因子
ACS Nano
·
2022
·
IF 18.027
Yu Wang,Lingyu Sun,Guopu Chen,Hanxu Chen,Yuanjin Zhao
Ionic hydrogels have attracted extensive attention because of their wide applicability in electronic skins, biosensors, and other biomedical areas. Tremendous effort is dedicated to developing ionic hydrogels with improved detection accuracy and multifunctionality. Herein, we present an inverse opal scaffold-based structural color ionic hydrogel with the desired features as intelligent patches for wound management. The patches were composed of a polyacrylamide-poly(vinyl alcohol)-polyethylenimine-lithium chloride (PAM–PVA-PEI-LiCl) inverse opal scaffold and a vascular endothelial growth factor (VEGF) mixed methacrylated gelatin (GelMA) hydrogel filler surface. The scaffold imparted the composite patches with brilliant structural color, conductive property, and freezing resistance, while the VEGF-GelMA surface could not only prevent the ionic hydrogel from the interference of complex wound conditions but also contribute to the cell proliferation and tissue repair in the wounds. Thus, the hydrogel patches could serve as electronic skins for in vivo wound healing and monitoring with high accuracy and reliability. These features indicate that the proposed structural color ionic hydrogel patches have great potential for clinical applications.
研究领域:
生物医学工程
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材料科学
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组织工程
·
伤口愈合
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产品关联
DOI
PubMed
高影响因子
Journal of Water Process Engineering
·
2024
·
IF 6.3
Yuxuan Zhang,Zaosheng Lv,Wenjun Lv,Kangwei Zhang,Yang Lei,Yanfen Huang
Hydrogels have become a focal point in oil-water separation technologies owing to their pronounced hydrophilicity and manageable viscosity. However, traditional hydrogels often suffer from subpar physical and mechanical strength. Our research introduces a semi-interpenetrating network (Semi-IPN) hydrogel composed of polyacrylamide and chitosan network (PAM/CSn), distinguished by both physical and chemical cross-linking to boost mechanical robustness. Experimental results demonstrate that polyacrylamide/chitosan network-SiO 2 @stainless steel mesh (PAM/CSn-SiO 2 @SSM) not only displays exceptional superoleophobic properties underwater (OCA >150°) but also maintains over 99 % separation efficiency across >20 cycles in diverse conditions including acidic, alkaline, and saline environments. In addition, the composite coating excelled in self-cleaning of viscous oils and in oil-in-water emulsion oil removal (92 %). Compared with previous polyacrylamide (PAM) hydrogels, our composite coating also possesses corrosion resistance to acids and alkalis and excellent ability to separate oil-water mixtures in harsh environments. These findings underscore the substantial potential of polyacrylamide/chitosan network-SiO 2 @stainless steel mesh (PAM/CSn-SiO 2 @SSM) composites in enhancing oil-water separation techniques.
研究领域:
材料科学
·
环境科学
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产品关联
DOI
高影响因子
Nanoscale Advances
·
2022
·Vol. 4
·期 19
·4169-4174
·
IF 5.598
Yi Gao,Shaofeng Liang,Shuangliang Zhao,Wei Gao,Zequan Li,Muqun Wang,Hong Li,Xiangning He,Riyao Cong,Hailin Diao,Chuwang Su,Xiaoying Xie
The emergence of cooling technology has brought about huge social benefits to society, but it is also accompanied by the serious problem of energy consumption. In countries close to the equator, intense solar radiation is accompanied by unbearable high temperatures and strong ultraviolet radiation. Therefore, we prepared a simple hydrogel with good evaporative cooling, which can work continuously and has good UV absorption, to solve the indoor cooling and UV radiation problems. Polyacrylamide (PAM) in the hydrogel provides a mechanically strong backbone, and polyethylene glycol (PEG) slows water loss and provides the hydrogel with the ability to reflect infrared light. Lithium bromide (LiBr) is a highly efficient water vapor absorbent, which can provide the hydrogel with water regeneration capability. Carbon dots (CDs) can provide excellent UV absorption for hydrogels, and CDs (4.28 kJ kg−1 K−1) have a higher specific heat capacity than water (4.20 kJ kg−1 K−1), which can store more heat for a better indoor cooling effect. The composite hydrogel has a good prospect of application in the windows of residential and high-rise buildings.
研究领域:
材料科学
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环境科学
·
化学工程
·
能源技术
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DOI
PubMed
高影响因子
Nanoscale
·
2022
·Vol. 14
·期 9
·3545-3553
·
IF 8.307
Di Sun,Jie Liu,Lijuan Xue,Li Li,Daoyin Xie,Shilin Li,Shengmei Li,Xinlian Wang,Dongtao Yin,Zhaoqi Ren,Ru Bai,Wei Guo,Ying Liu,Chunying Chen
Superficial thrombophlebitis is one of the most significant complications of superficial vein thrombosis. Rapid imaging and mapping with high resolution is particularly important for accurate diagnosis so as to carry out treatment as soon as possible. Ultrasound imaging technology has been used extensively because of the low-cost, minimal invasiveness, and convenient application in clinical practice. And the ultrasonic couplant is an essential component in ultrasound examination. However, when imaging superficial structures, traditional liquid ultrasonic couplants often produce inadequate results. In this study, we investigate whether a hydrogel membrane can be used to improve the imaging of superficial vessels. To this end, we generated a polyacrylamide-bacterial nanocellulose hydrogel membrane (PAM-BC) that efficiently forms at 60 °C in only 10 min by redox polymerization. With PAM-BC-2.5, it was possible to acquire high resolution intravascular ultrasound images to assess superficial vessels in humans and the superficial vasculature in rats and miniature pigs using various brands of ultrasound instruments. The PAM-BCs represent a new, solid ultrasonic membrane which is suitable for diagnosing disease in superficial vessels.
研究领域:
医学影像
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生物医学工程
·
材料科学
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产品关联
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2022
·
IF 6.911
Tianrun Zhang,Xiuwei Xuan,Mingji Li,Cuiping Li,Penghai Li,Hongji Li
Molecularly imprinted polymer (MIP) membranes prepared in situ present several advantages: they maintain the original morphology, adhere strongly to the collector, and exhibit a controllable structure. In this study, a Ni-polyacrylamide (PAM)-MIP matrix was fabricated in situ on glassy carbon via the one-step electro-polymerization of AM monomers in the presence of Ni and template molecules. Ni 2+ ions were introduced as oxidants to promote AM polymerization and bulking agents to fabricate a three-dimensional porous PAM-MIP matrix. The Ni-PAM-based MIP sensor exhibited a quantitative dual response toward dopamine (DA) and adenine (Ade) in the pH range of 5.0–9.0. The linear concentration range changed depending on the pH environment, and the concentrations of DA and Ade ranged from 0.6 to 200 μM and from 0.4 to 300 μM, respectively. The ranges of detection limits (S/N = 3) were 0.12–0.37 μM for DA and 0.15–0.36 μM for Ade. In addition, the dual-MIP sensor exhibited high reliability in the detection of DA and Ade in human serum owing to its excellent anti-interference ability and long-term stability. The technique developed in this study is expected to facilitate the construction of multi-target response electrochemical biosensors and the reliable determination of small molecules with high selectivity and stability.
研究领域:
分析化学
·
生物传感器
·
材料科学
·
电化学
·
生物分析化学
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产品关联
DOI
PubMed
高影响因子
MICROPOROUS AND MESOPOROUS MATERIALS
·
2021
·
IF 5.455
Min Zhang,Shenyong Ren,Qiaoxia Guo,Baojian Shen
TS-1 is a typical microporous zeolite , which is hindering the timely diffusion of reaction products and is likely to cause secondary side reactions in heterogeneous catalytic reaction due to the micropore size. However, zeolite TS-1 with hierarchical pores of micropores and mesoporous/macropores can solve this problem to a great extent. In view of this, hierarchical zeolite TS-1 is synthesized by using tetrapropylammonium hydroxide (TPAOH) as structure directing agent and polyacrylamide (PAM) as a hierarchical pore-directing template. The texture property of hierarchical TS-1 can be optimized by changing the amount of PAM in the synthesis gel. With the increase of PAM content, the pore volume and specific surface area of the hierarchical zeolite TS-1 increases. Compared with the conventional TS-1, the hierarchical TS-1 samples have obvious pore size distribution in the range of 20–80 nm. It is interesting that the particle size of TS-1 decreases after adding PAM. When the hierarchical TS-1 is applied to the epoxidation reaction of 1-hexene with H 2 O 2 , the selectivity of the desired product 1,2-epoxyhexane is significantly increased.
研究领域:
催化
·
材料科学
·
化学工程
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DOI
高影响因子
Polymers
·
2023
·Vol. 15
·期 12
·2744
·
IF 5
Yue Jiang,Shanzhu Guo,Jingjing Jiao,Long Li
The treatment of osteochondral defects remains challenging due to the limited healing capacity of cartilage and the poor results of traditional methods. Inspired by the structure of natural articular cartilage, we have fabricated a biphasic osteochondral hydrogel scaffold using a Schiff base reaction and a free radical polymerization reaction. Carboxymethyl chitosan (CMCS), oxidized sodium alginate (OSA), and polyacrylamide (PAM) formed a hydrogel (COP) as the cartilage layer, while hydroxyapatite (HAp) was incorporated into the COP hydrogel to obtain a hydrogel (COPH) as an subchondral bone layer. At the same time, hydroxyapatite (HAp) was incorporated into the COP hydrogel to obtain a hydrogel (COPH) as an osteochondral sublayer, combining the two to obtain an integrated scaffold for osteochondral tissue engineering. Interlayer interpenetration through the continuity of the hydrogel substrate and good self-healing properties due to the dynamic imine bonding of the hydrogel resulted in enhanced interlayer bond strength. In addition, in vitro experiments have shown that the hydrogel exhibits good biocompatibility. It shows great potential for osteochondral tissue engineering applications.Keywords:biphasic scaffolds;self-healing hydrogel;osteochondral repair
研究领域:
组织工程
·
生物材料
·
骨科
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）