CHEMICAL ENGINEERING JOURNAL
·
2023
·
IF 15.1
Chunmei Li,Pingfan Zhang,Fang Gu,Lei Tong,Jizhou Jiang,Yan Zuo,Hongjun Dong
A new Au-θ-Al 2 O 3 /Au/PCN hybrid is fabricated by the collaborative design strategy of active site and assembled structure, which achieves highly efficient photocatalytic CO 2 reduction (CO 2 R) performance. The energy band structure of PCN is adjusted significantly owing to the strong coupling effect of Au-θ-Al 2 O 3 /Au nanosheets intercalated in PCN nanosheets. The result brings about up-shift of CB, VB and Fermi energy level overall and decrease of bandgap, which is in favor of harvesting visible light, exciting VB electrons and increasing reduction ability. In addition, the multiple built-in electric fields are induced by electron diffusion effect between structural units, due to the tight interface contact in the Au-θ-Al 2 O 3 /Au/PCN hybrid, which can effectively promote the separation of photoinduced charge carriers. Furthermore, the atomically dispersed Au atoms confined by the oxygen vacancies in Au-θ-Al 2 O 3 /Au can provide the plenty of effective active sites for CO 2 R reaction, and meanwhile, the localized surface plasmon resonance (LSPR) effect induced by Au nanoparticles on Au-θ-Al 2 O 3 /Au can improve the visible light response and produce abundant hot electrons to contribute to CO 2 R reaction. As a consequence, the average CO evolution rate of over the optimizing 10 %-Au-θ-Al 2 O 3 /Au/PCN hybrid (7.76 μmol g -1 h −1 ) reaches up to 3.53 times than that of PCN (2.20 μmol g -1 h −1 ) in the CO 2 R reaction, and maintains basically stable operation within 7 cycles of running, suggesting the superior activity and recyclability.
研究领域:
材料科学
·
环境科学
✓ 已验证
产品关联
DOI
高影响因子
DRUG METABOLISM AND DISPOSITION
·
2024
·Vol. 52
·期 7
·597-605
·
IF 4.4
Ting Wu,Lu Li,Wenhong Zhou,Guofang Bi,Xiaowen Jiang,Manlan Guo,Xiao Yang,Jianhong Fang,Jianxin Pang,Shicheng Fan,Huichang Bi
Pregnane X receptor (PXR) is essential in the regulation of liver homeostasis, and the gut microbiota is closely linked to liver physiologic and pathologic status. We previously found that activation of PXR significantly promotes liver enlargement through interaction with yes-associated protein (YAP). However, whether gut microbiota contributes to PXR-induced hepatomegaly and the involved mechanisms remain unclear. In this study, C57BL/6 mice were administered the mouse-specific agonist pregnenolone 16α-carbonitrile (PCN) for 5 days. Depletion of gut microbiota was achieved using broad-spectrum antibiotics (ABX) and fecal microbiota transplantation (FMT) was performed to restore the gut microbia. The composition of gut microbiota was analyzed by 16S rRNA sequencing, while the expression of PXR, YAP, and their downstream target genes and proteins were assessed. The results indicated that PCN treatment altered the composition and abundance of specific bacterial taxa. Furthermore, depletion of gut microbiota using ABX significantly attenuated PCN-induced hepatomegaly. FMT experiments further demonstrated that the fecal microbiota from PCN-treated mice could induce liver enlargement. Mechanistic studies revealed that ABX treatment impeded the PXR and YAP activation induced by PCN, as evidenced by decreased expression of PXR, YAP, and their downstream targets. Moreover, alterations in PXR and YAP activation were likely contributing to hepatomegaly in recipient mice following FMT from PCN-treated mice. Collectively, the current study demonstrated that gut microbiota is involved in PCN-induced hepatomegaly via regulating PXR and YAP activation, providing potential novel insights into the involvement of gut microbiota in PXR-mediated hepatomegaly.
研究领域:
药理学
·
微生物学
·
肝脏病学
·
分子生物学
✓ 已验证
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2022
·
IF 8.008
Jinzheng Zhang,Shuailong Zhou,Shiyi Tan,Kangyan Yi,Mengya Jin,Qian Shi,Fen Wu,Nannan Liu
Pore structure-based analytical techniques have great potential applications for the detection of biological molecules. However, the sophistication of traditional pore sensors is restricted in their applicability of analytical chemistry due to a lack of effective carrier probes. Here, we used porous coordination network-224 (PCN-224) composite probes in conjunction with a glass nanopipette (GN) as a sensing platform. The sensor exhibits a good fluorescence signal and a change in GN’s ionic current at the same time. Due to the volume exclusion mechanism coming from PCN-224, the detection limit of target DNA reaches 10 fM in a GN with a diameter of up to ca. 260 nm, outperforming a simple probe. The structure of the composite probe is optimized by the probe’s pairing efficiency. Furthermore, the sensor can also discriminate between 1-, 3-, and 5-mismatch DNA sequences and capture the target DNA from a complex mixture. Based on the GN platform, a series of techniques for detecting biomolecules are expected to emerge because of its simplicity, robustness, and universality by incorporating advanced nanoprobes.
研究领域:
分析化学
·
生物传感器
·
纳米技术
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2025
·
IF 13.2
Yaxin Li,Wei Qin,Xinyang Li,Qi Zhu,Fu-quan Bai,Tao Tian
It is highly desirable to enhance the charge separation and introduce catalytic functions for efficient water splitting into hydrogen on g-C 3 N 4 . Herein, heterojunctions of PCN-222(M)/g-C 3 N 4 -AA were successfully synthesized through the in-situ hydrothermal method, where metallic ions (M) were strategically incorporated into porphyrinic metal-organic framework (MOF) ligands. This heterojunction design strategy is considered to establish directional charge transfer channels for photogenerated electron-hole (e − -h + ) pairs and thereby enhance its separation efficiency. Remarkably, the 1 % PCN-222(Pd)/g-C 3 N 4 -AA composite demonstrated superior photocatalytic hydrogen evolution performance, achieving a rate of 2338.17 μmol·g −1 ·h −1 . This rate was 3.23 times higher than that of pure g-C 3 N 4 and 2.79 times higher than that of g-C 3 N 4 -AA. The composite morphology was characterized by a series of physicochemical properties. Bandgap analysis via Tauc plot revealed that metal ion doping in the porphyrin center effectively modulates the HOMO-LUMO levels at the composite interface. First-principles calculations coupled with hydrogen adsorption free energy analysis elucidated how interfacial engineering between PCN-222(M) and g-C 3 N 4 -AA governs heterostructure formation and charge transfer pathways. This work systematically deciphers the synergistic effects of bimetallic MOF integration and provides insights into the underlying photocatalytic reaction mechanism.
研究领域:
材料科学
·
纳米技术
·
催化
·
能量转换
·
物理化学
✓ 已验证
产品关联
DOI
高影响因子
INORGANIC CHEMISTRY COMMUNICATIONS
·
2025
·
IF 5.4
Yapu Yang,Yalin Chang,Yueshang Wang,Yinuo Gai,Jun Ma,Pengfei Shi
Phototherapy is rapidly evolving in the treatment of bacterial infections to counter the dangers of bacterial evolution and the development of bacterial resistance due to the misuse of antibiotics. Herein, we report a nano-antimicrobial platform (PCN-224-IrO 2 -HA), constructed by iridium oxide nanoparticles and metal-organic framework PCN-224. PCN-224-IrO 2 -HA is capable of the photodynamic therapy (PDT), which can induce bacterial death by converting surrounding O 2 to reactive oxygen species under laser irradiation. Meanwhile, IrO 2 can catalyse the decomposition of H 2 O 2 to generate O 2 and enhance PDT. Meanwhile, IrO 2 can rapidly convert light energy into thermal energy under laser irradiation, so that it is able to kill bacteria by photothermal therapy (PTT). Photodynamic therapy synergised with photothermal therapy significantly inhibit the growth of bacteria, providing a new idea for phototherapy to treat bacterial infections.
研究领域:
纳米医学
·
抗菌治疗
·
光疗
·
材料科学
·
传染病
✓ 已验证
产品关联
DOI
高影响因子
TALANTA
·
2025
·
IF 6.1
Lin Chai,Haoyu Chen,Xing Yang,Jing Liu,Qiquan Yang,Shu Huang,Min Tang,Xiaohua Zhu,Haitao Li,Youyu Zhang,Meiling Liu
Ratiometric fluorescent nanoplatforms with self-calibrating capabilities offer enhanced detection accuracy by minimizing environmental interference. However, challenges such as signal cross-interference and nanomaterial instability persist in complex biological systems. To address these issues, we developed a glutathione (GSH)-responsive ratiometric fluorescent nanoprobe by integrating a porphyrin-based metal-organic framework (PCN-224) with MnO 2 nanosheets (NSs). The PCN-224 serves as both a nanocarrier and luminescent moiety, while MnO 2 acts as oxidase (OXD) mimetic nanozyme and the reactive site for GSH. The ratiometric detection mechanism relies on the intrinsic fluorescence of PCN-224 (emission at 655 nm) and the OXD-like activity of PCN-224@MnO 2 , which catalyzes the oxidation of o -phenylenediamine (OPD) to generate fluorescent 2,3-diaminophenazine (DAP, emission at 570 nm). Upon GSH exposure, the redox reaction between GSH and MnO 2 produces Mn 2+ , quenching the OXD-like activity and diminishing the DAP fluorescence, while the PCN-224 fluorescence recovers due to MnO 2 decomposition. The nanoplatform exhibits a wide detection range spanning from 0.1 to 60 μM with a low detection limit of 15 nM and high selectivity. Furthermore, it enables accurate GSH quantification in complex biological matrices, including human serum and cell lysates, and facilitates real-time monitoring of endogenous and exogenous GSH in living cells via fluorescence imaging. This study not only presents sensitive and reliable platform for GSH detection, but also overcomes key limitations in ratiometric sensing, such as signal interference and nanomaterial instability, by utilizing a stimuli-responsive fluorescent nanoprobe design.
研究领域:
分析化学
·
纳米技术
·
生物化学
·
生物传感器
·
荧光成像
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
RSC Advances
·
2023
·Vol. 13
·期 16
·11215-11224
·
IF 3.9
Haidi Feng,Lu Zhao,Zhiqiang Bai,Zhihui Xin,Chaoyu Wang,Lizhen Liu,Jinping Song,Haifei Zhang,Yunfeng Bai,Feng Feng
Active-targeted nanoplatforms could specifically target tumors compared to normal cells, making them a promising therapeutic agent. The aptamer is a kind of short DNA or RNA sequence that can specifically bind to target molecules, and could be widely used as the active targeting agents of nanoplatforms to achieve active-targeted therapy of tumors. Herein, an aptamer modified nanoplatform DOX@PCN@Apt-M was designed for active-targeted chemo-photodynamic therapy of tumors. Zr-based porphyrinic nanoscale metal organic framework PCN-224 was synthesized through a one-pot reaction, which could produce cytotoxic 1O2 for efficient treatment of tumor cells. To improve the therapeutic effect of the tumor, the anticancer drug doxorubicin (DOX) was loaded into PCN-224 to form DOX@PCN-224 for tumor combination therapy. Active-targeted combination therapy achieved by modifying the MUC1 aptamer (Apt-M) onto DOX@PCN-224 surface can not only further reduce the dosage of therapeutic agents, but also reduce their toxic and side effects on normal tissues. In vitro, experimental results indicated that DOX@PCN@Apt-M exhibited enhanced combined therapeutic effect and active targeting efficiency under 808 nm laser irradiation for MCF-7 tumor cells. Based on PCN-224 nanocarriers and aptamer MUC1, this work provides a novel strategy for precisely targeting MCF-7 tumor cells.
研究领域:
纳米技术
·
癌症治疗
·
材料科学
·
生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2024
·
IF 12.2
Wei Chen,Xiayu Peng,Lichao Kang,Shengnan Dong,Jian Zhang,Yunfeng Zhao,Fengxia Sun
The development of a versatile platform for bacterial assay and elimination is urgently needed due to the danger that bacteria pose to human life. Here, we synthesized a trimetallic deposition and horseradish peroxidase (HRP)-embedded porous coordination network-224 hybrid nanozymes (PCN-224 @AuPdPt@HRP) with outstanding peroxidase activity and fluorescence quenching ability. On this basis, we designed a dual recognition strategy-driven colorimetric-fluorescence dual-mode detection platform using Listeria monocytogenes as a pattern analyte. The platform consisted of an aptamer-modified PCN-224 @AuPdPt@HRP (PCN-224 @AuPdPt@HRP@Aptamer) specifically recognizing Listeria monocytogenes and vancomycin-coated 96-well plates. In the presence of vancomycin, which has the ability to recognize and inactivate gram-positive bacteria, the significant peroxidase activity of PCN-224 @AuPdPt@HRP@Aptamer in the precipitate was able to catalyze the color change of the substrate by H 2 O 2 . Meanwhile, the residual PCN-224 @AuPdPt@HRP@Aptamer in the supernatant was able to change the fluorescence of fluorescein-labeled deoxyribonucleic acid (FAM-DNA). In summary, this paper presents a multifunctional platform capable of detecting and eliminating residual bacteria in real environments. This strategy is expected to facilitate the development of multifunctional biosensors based on metal–organic framework probes and also provide environmental health.
研究领域:
生物技术
·
分析化学
·
微生物学
·
材料科学
·
环境健康
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ENVIRONMENTAL TECHNOLOGY
·
2022
·
IF 3.475
Kongyan Luo,Long Chen,Yuanyi Zhao,Guyu Peng,Zhaobo Chen,Qian Chen
Zinc oxide nanoparticles (ZnO NPs) show adverse impacts on aerobic denitrifying bacteria, little is known about the response of these bacteria to ZnO NPs exposure at cellular level. This study assessed the multiple responses of Pseudomonas aeruginosa PCN-2 under ZnO NPs exposure. We demonstrated that ZnO NPs exposure could inhibit the intracellular metabolism and stimulate the antioxidant defence capability of PCN-2. At lower exposure concentration (5 mg/L), exogenous ROS generated and resulted in the inhibition of pyruvate metabolism and citrate cycle, which caused deficient energy for aerobic denitrification. At higher concentrations (50 mg/L), endogenous ROS additionally generated and triggered to stronger down-regulation of oxidative phosphorylation, which caused suppressed electron transfers for aerobic denitrification. Meanwhile, ZnO NPs exposure promoted EPS production and biofilm formation, and antioxidases was especially particularly stimulated at higher concentration. Our findings are significant for understanding of microbial bacterial susceptibility, tolerance and resistance under the exposure of ZnO NPs.Highlights Aerobic denitrification is suppressed with increased ZnO NPs concentrations.ZnO NPs induce inhibition of pyruvate metabolism and citrate cycle at 5 mg/L.Higher ZnO NPs concentration induces the generation of endogenous and exogenous ROS.Higher NPs concentration leads to the trigger of antioxidant system.ZnO NPs stimulate metabolisms of EPS production.
研究领域:
环境微生物学
·
转录组学
·
纳米毒理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2022
·
IF 5.518
Jinxing Hou,Guodong Dai,Jiuwu Wang,Xian Sun,Yue Situ,Hong Huang
A novel ternary polythiophene/Ag/protonated g-C 3 N 4 nanosheets (PTh/Ag/pCN) nanocomposite was constructed by electrostatic self-assembly strategy. The as-prepared 5% PTh/Ag/pCN exhibits excellent visible light photocatalytic performance toward methylene orange (MO), which is 6 and 3.7 times higher than pCN and PTh, respectively. The result was dominantly ascribed to the forming of Z-scheme heterojunction due to the synergetic effects of pCN, Ag and PTh, which is conductive to the separation of photogenerated charges. Meanwhile, the radical trapping experiments and ESR techniques proved that the photogenerated holes and superoxide radicals are vital reactive substances. This study provides a powerful approach to enhance catalytic activity of conjugated polymer photocatalyst .
研究领域:
材料科学
·
环境科学
·
光催化
✓ 已验证
产品关联
DOI
高影响因子
Nanomedicine-Nanotechnology Biology and Medicine
·
2023
·
IF 5.4
Jihua Tian,Jing Wang,Huanyu Xu,Bocheng Zou,Weihao Chen,Yulong Liu,Jingshu Chen,Ruiping Zhang
Rapamycin has great potential in the antitumor application, but its therapeutic effect is seriously affected by poor water solubility, targeting ability, and low bioavailability. Here, we constructed a novel composite nanomaterial with PCN-224 as a drug carrier and loaded rapamycin, named [email protected] @HA. The nanoplate not only improves targeting, but also synergizes rapamycin with PCN-224 to effectively promote tumor cell apoptosis , which subsequently causes immunogenic cell death (ICD), and shows strong therapeutic effect in 4T1 breast cancer model. The treatment effect depends on three main points:(i)Proapoptotic effect of rapamycin on tumor cells;(ii)ROS production by PCN-224-mediated photodynamic therapy;(iii)ICD induced DC maturation, increased immune response and promoted T cell proliferation and differentiation. This nanoplate offers potential antitumor efficacy in combination with chemotherapy, photodynamic therapy, and immunotherapy .
研究领域:
癌症研究
·
纳米技术
·
免疫疗法
·
药学
·
生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2020
·
IF 3.232
Wen Zheng,Jiahui Liu,Danyang Yi,Yadi Pan,Yijuan Long,Huzhi Zheng
Ficin has been reported to possess peroxidase activity, but its applications in some respects have been limited because of its relatively low activity. Herein, a mesoporous metal-organic framework, PCN-333(Fe), was synthesized, which was selected to encapsulate ficin to form [email protected] (Fe). Compared with ficin, the peroxidase-like activity of [email protected] (Fe) toward 3,3′,5,5′-tetramethylbenzidine (TMB) oxidation was about 3 times increase in the presence of H 2 O 2 , and followed classical Michaelis-Menten model. The kinetic parameters showed that stronger affinity and higher catalytic constant ( K cat ) of [email protected] (Fe) to both TMB and H 2 O 2 compared with ficin, and K cat of [email protected] (Fe) was increased by 3.65 folds and 3.59 folds for TMB and H 2 O 2 , respectively. Taking advantages of higher catalytic property of [email protected] (Fe), we developed a colorimetric method with high sensitivity and selectivity to detect glucose, which displayed a good linear response toward glucose in the range of 0.5–180 μM with a limit of detection of 97 nM. Furthermore, [email protected] (Fe) has been proven to successfully detect glucose in human serum, implying its great potentialities and wide applications as peroxidase mimics.
研究领域:
生物化学
·
分析化学
·
材料科学
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）