Nature Communications
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2022
·Vol. 13
·期 1
·1-12
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IF 17.694
Li Juan-Juan,Hu Yuqing,Hu Bing,Wang Wenbo,Xu Haiqi,Hu Xin-Yue,Ding Fei,Li Hua-Bin,Wang Ke-Rang,Zhang Xinge,Guo Dong-Sheng
Diabetic wound is one of the most intractable chronic wounds that is prone to bacterial infection. Hypoxia is an important feature in its microenvironment. However, it is challenging for antimicrobial therapy to directly apply the existing hypoxia-responsive drug delivery systems due to the active targeting deficiency and the biofilm obstacle. Herein, we customizes a hypoxia-responsive carrier, lactose-modified azocalix[4]arene (LacAC4A) with the ability to actively target and inhibit biofilm. By loading ciprofloxacin (Cip), the resultant supramolecular nanoformulation Cip@LacAC4A demonstrates enhanced antibacterial efficacy resulting from both the increased drug accumulation and the controlled release at the site of infection. When applied on diabetic wounds together with multidrug-resistant Pseudomonas aeruginosa infection in vivo, Cip@LacAC4A induces definitely less inflammatory infiltration than free Cip, which translates into high wound healing performance. Importantly, such design principle provides a direction for developing antimicrobial drug delivery systems.
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CHEMICAL ENGINEERING JOURNAL
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2022
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IF 16.744
Qianqian Guo,Yongjun Luo,Honglei Guo,Tianyu Lan,Shengquan Wang,Kedui Geng,Xin Lu,Ling Tao,Xiangchun Shen
Lung infection associated with drug-resistant P. aeruginosa and their wide spread have become a serious problem to public health. Effective antibacterial strategy without antibiotics must be developed for treatment of these infections. To combat pneumonia infected by multidrug-resistant P. aeruginosa (MRPA), a novel antibacterial nanocomoposite of [email protected] r PEGMA was prepared. [email protected] r PEGMA could bind with P. aeruginosa through the interaction betweeen PBA r PEGMA and bacteria proved by alizarin red assay. The nanocomposite could relieve inflammtory response in MRPA-infected macrophages by downregulating MAPK and NF κ B signal-transduction pathways. [email protected] r PEGMA showed well photo-thermal conversion capacity leading to the temperature increasing from 20 to 80 °C under near-infrared (NIR) irradiation for 5 min. The photo-thermal capacity endowed the nanocomposites efficient antibacterial activity against MRPA. Simultaneously, [email protected] r PEGMA with NIR irradiation could kill biofilm bacteria while traditional antibiotics were non-effetive. [email protected] r PEGMA with NIR irradiation could treat MRPA-related lung infection. The nanocomposite with NIR reduced the inflammatory cytokines of TNF-α, IL-1β, and IL-6 by downregulating the MAPK signal pathway in bronchoalveolar lavage fluids. The number of P. aeruginosa and expression of NF-κB p65 in lung also decreased. Above all, our study provides a non-antibiotic therapeutic strategy in clinical application and demonstrated that [email protected] r PEGMA with NIR irradiation showed a potential treatment for drug-resistant P. aeruginosa infection in Lung.
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CHEMOSPHERE
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2023
·
IF 8.8
Jie-wen Ma,Gui-wen Liu,Jia-Yu Zhai,Ke-qian Zhao,Ya-qing Wu,Rui-lian Yu,Gong-ren Hu,Yu Yan
Roxarsone (3-nitro-4-hydroxyphenylarsonic acid, Rox), a widely used organoarsenical feed additive, can enter soils and be further biotransformed into various arsenic species that pose human health and ecological risks. However, the pathway and molecular mechanism of Rox biotransformation by soil microbes are not well studied. Therefore, in this study, we isolated a Rox-transforming bacterium from manure-fertilized soil and identified it as Pseudomonas chlororaphis through morphological analysis and 16S rRNA gene sequencing. Pseudomonas chlororaphis was able to biotransform Rox to 3-amino-4-hydroxyphenylarsonic acid (3-AHPAA), N -acetyl-4-hydroxy- m -arsanilic acid (N-AHPAA), arsenate [As(V)], arsenite [As(III)], and dimethylarsenate [DMAs(V)]. The complete genome of Pseudomonas chlororaphis was sequenced. PcmdaB, encoding a nitroreductase, and PcnhoA, encoding an acetyltransferase, were identified in the genome of Pseudomonas chlororaphis . Expression of PcmdaB and PcnhoA in E. coli Rosetta was shown to confer Rox(III) and 3-AHPAA(III) resistance through Rox nitroreduction and 3-AHPAA acetylation , respectively. The PcMdaB and PcNhoA enzymes were further purified and functionally characterized in vitro . The kinetic data of both PcMdaB and PcNhoA were well fit to the Michaelis-Menten equation, and nitroreduction catalyzed by PcMdaB is the rate-limiting step for Rox transformation. Our results provide new insights into the environmental risk assessment and bioremediation of Rox(V)-contaminated soils.
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JOURNAL OF HAZARDOUS MATERIALS
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2023
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IF 13.6
Jinfeng Lu,Yuexian Liu,Ruili Zhang,Zhengyi Hu,Kai Xue,Biya Dong
Controversial results are reported whereby biodegradation of polycyclic aromatic hydrocarbons (PAHs) can be promoted or inhibited by biochar amendment of soil. Metabolomics was applied to analyze the metabolic profiles of amendment with biochar (BB) and biochar inoculated with functional bacteria ( Pseudomonas putida ) (BP) involved in phenanthrene (PHE) degradation. Additionally, metagenomic analysis was utilized to assess the impact of different treatments on PHE degradation by soil microorganisms. Results indicated that BB treatment decreased the PHE biodegradation of the soil indigenous bacterial consortium, but BP treatment alleviated this inhibitory effect. Metabolomics revealed the differential metabolite 9-phenanthrol was absent in the BB treatment, but was found in the control group (CK), and in the treatment inoculated with the Pseudomonas putida (Ps) and the BP treatment. Metagenomic analysis showed that biochar decreased the abundance of the cytochrome P450 monooxygenase (CYP116), which was detected in the Pseudomonas putida , thus alleviating the inhibitory effect of biochar on PHE degradation. Moreover, a noticeable delayed increase of functional gene abundance and enzymes abundance in the BB treatment was observed in the PHE degradation pathway. Our findings elucidate the mechanism of inhibition with biochar amendment and the alleviating effect of biochar inoculated with degrading bacteria.
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BIOCHEMICAL ENGINEERING JOURNAL
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2019
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IF 3.371
Xiaoqiang Jia,Yun He,Dawei Jiang,Chang Liu,Wenyu Lu
Polycyclic aromatic hydrocarbons (PAHs) like phenanthrene are harmful to human health and are difficult to eliminate from environments. In this study, a phenanthrene degrading strain, Pseudomonas aeruginosa PH1, was isolated from oil-contaminated soil. An engineered co-culture consortium was subsequently constructed comprising two engineered bacterial strains, Escherichia coli HY1 and P. aeruginosa PH2, for use in phenanthrene bioremoval. E. coli HY1 harbored two modules of terminal dioxygenases and an electron transfer chain, while P. aeruginosa PH2 contained a module of catechol 1,2-dioxygenase. Phenanthrene removal rates by the wild-type degrader, P. aeruginosa PH1, were 45%, 43%, and 81%, in mineral salt medium supplemented with 100 mg/L phenanthrene, 100 mg/L phenanthrene and 2% (wt/vol) oil, and 100 mg/L phenanthrene and 2% (vol/vol) n -hexadecane, respectively. In contrast, the engineered co-culture consortium removed 71%, 80%, and 88% of the phenanthrene in the corresponding treatments, thus achieving much higher removal efficiencies. Moreover, gas chromatography-mass spectrometry confirmed that the engineered co-culture consortium first oxidized phenanthrene to 9,10-dihydroxy phenanthrene and 1,2-dihydroxy phenanthrene and then metabolized these intermediates via catechol to carbon dioxide and water. During this process, E. coli HY1 dominated the initial oxidation steps, while P. aeruginosa PH2 was primarily responsible for ring cleavage. These results indicate that the engineered co-culture consortium described here represents a potential alternative and attractive means to remove PAHs from natural environments.
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AMB Express
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2018
·Vol. 8
·期 1
·1-13
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IF 1.719
Liang Jibei,Cheng Tao,Huang Yi,Liu Jianhua
Enhanced bioremediation is a favorable approach for petroleum pollutant cleanup, which depends on the growth of oil-eating microorganisms. In this study, we show that, by using the modified T-RFLP (mT-RFLP) methodology, one of the four major microbial populations derived from oil sludge has failed to propagate in MS medium supplemented with 2% yeast extract (YE). rDNA sequence-based analysis indicated that the four populations were Donghicola sp. CT5, Bacillus sp. CT6, Alcaligenes sp. CT10, and Pseudomonas sp. ZS1. Four purified strains grow well individually in MS medium supplemented with 2% YE, suggesting that ZS1 growth is antagonized by other strains. Co-growth analysis using mT-RFLP methodology and plate inhibitory assay indicated that ZS1 exhibited antagonistic effect against CT5 and CT6. On the other hand, co-growth analysis and plate inhibition assay showed that CT10 antagonized against ZS1. To investigate the potential compounds responsible for the antagonism, supernatant of CT10 culture was subjected to GC–MS analysis. Analysis indicated that CT10 produced a number of antimicrobial compounds including cyclodipeptide c-(L-Pro-L-Phe), which was known to inhibit the growth of Pseudomonas sp. Growth test using the purified c-(L-Pro-L-Phe) from CT10 confirmed its inhibitory activity. We further showed that, using both gravimetric and GC analysis, CT10 antagonism against the oil-eating ZS1 led to the diminishing of crude oil degradation. Together, our results indicate that bioremediation can be affected by environmental antagonists.
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Water
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2018
·Vol. 10
·期 10
·1376
·
IF 2.069
Dan Zhong,Fu He,Wencheng Ma,Yixing Yuan,Ziqiang Wang
The present work intended to use goethite, one of the main compositions of pipe deposit, to combine with H2O2to degrade TCE or disinfect drinking water. Goethite exhibited excellent degradation performance for TCE, outstanding inactivation ability for Pseudomonas and the disinfection effect for filter water of water treatment plants. The TCE degradation efficiency could reach 87.4%, while the inactivation efficiency of Pseudomonas and the bacterial mortality rate in filtered water were more than 99.9% in this study. In order to effectively reduce the disinfection by-products (chlorine-resistant bacteria) and conduct permanent disinfection, the Fenton-like and chlorine combined disinfection method was also investigated. Experimental results indicated that the bacteria could be effectively killed by this combined method and the chlorine residual was 0.41m g/L, ensuring sustainable disinfection. This work verified that the pipe deposit from the water distribution network is multifunctional, which is a potential candidate for pollutant removal and sterilization. The results could also offer theoretical support for water quality security in water distribution networks in the future.Keywords:water distribution network;growth ring;Fenton-like;disinfection;trichloroethylene
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Scientific Reports
·
2017
·Vol. 7
·期 1
·1-10
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IF 4.259
Yang Li,Cai Tianming,Ding Dahu,Cai Tianjin,Jiang Canlan,Li Hua,Yang Qian,Chen Liwei
2-hydroxy-1,4 naphthoquinone (lawsone) is widely used and induces environmental pollutions during its production and application. In the present study, a lawsone-degrading bacterium strain, LH-3 was successfully isolated from the activated sludge. Based on the 16S rRNA gene analysis, the strain LH-3 phylogenetically belonged to the Pseudomonas taiwanensis . It could degrade 200 mg L −1 lawsone completely in 9 h with an inoculum quantity of 1% (v/v). The effects of environmental conditions on the degradation process and the degradation pathway were systematically investigated. LH-3 could maintain its high degradation efficiency under high salt condition. The identified intermediates of salicylic acid, 2-hydroxy-4-oxo-chroman-2-carboxylic acid, and catechol elucidated the potential degradation pathway. Furthermore, the immobilized LH-3 strain cells prepared with alginate gel and biochar performed excellent stability in nine successive degradation runs. It could sucessfully survive in laboratory scale sequencing batch reactor and become to be the dominant species. This study clearly revealed that LH-3 could serve as an attractive candidate for the microbial remediation of lawsone-containing wastewater.
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Bioactive Materials
·
2024
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IF 18
Jinghuang Chen,Xianqing Tang,Qihan Sun,Xin Ji,Xingbo Wang,Zhendong Liu,Xu Zhang,Haijiao Xu,Fan Yang,Jian Sun,Xiurong Yang
Pseudomonas aeruginosa causes high morbidity and mortality in nosocomial infections, and newly approved antibiotics have been declining for decades. A green and universal deprotonation-driven strategy is used to screen the guanylic acid-metal ion coordination polymer nanoparticles (GMC), instead of the failure of binding occurs when specific metal ion participation. We find that the precise pH-dependent oxidase-like activity of GMC-2 orchestrates a duple symphony of immune modulation for Pseudomonas aeruginosa biofilm infections. Specifically, GMC-2-mediated reactive oxygen species (ROS) regulation triggers mitochondrial dysfunction and releases damage-associated molecular patterns, engaging pattern recognition receptors and resulting in endogenous innate immune activation. Meanwhile, GMC-2-triggered ROS generation in a mildly acidic biofilm environment destroys the biofilm, exposing exogenous pathogen-associated molecular patterns. GMC-2 cannot cause resistance for Pseudomonas aeruginosa compared with conventional antibiotics. In an infected implant mouse model, Pseudomonas aeruginosa biofilms were effectively eliminated by GMC-2-mediated triggering of innate and adaptive immunity. These findings provide a universal approach for facilitating the binding of biomolecules with metal ions and highlight the precise ROS-regulating platform plays a critical role in initiating endogenous and exogenous immune activation targeted for bacterial biofilm infection.
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Water
·
2024
·Vol. 16
·期 17
·2537
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IF 3
Bo Li,Hulong Liu,Xiaona Liu,Li Han,Jing Yang,Lingke Kang,Liuyuan Tang,Tianwei Qian
Bioremediation is a promising technique owing to its effectiveness, low cost, and environmental friendliness. Previous studies have focused on the degradation efficiency of polycyclic aromatic hydrocarbons (PAHs) in soil and water. However, the expression of PAH-catabolic genes in organisms involved in the degradation process has been rarely and unsystematically reported. In this study, a PAH-degrading strain—Pseudomonas aeruginosa(PQ249631)—was successfully isolated from coking-contaminated soil and used for PAH degradation in soil and water. Furthermore, the degradation of PAHs (naphthalene, fluorene, phenanthrene, anthracene, and pyrene) was investigated in single, binary, and mixture systems to explore the interaction of substrates. The results showed that when naphthalene was used as a cometabolite carbon source, the removal rates of fluorene, phenanthrene, anthracene, and pyrene increased from 14.33%, 17.25%, 6.61%, and 4.47% to 72.08%, 100.00%, 15.63%, and 6.63%, respectively. In a PAH mixture, the degradation rate of each PAH was higher when naphthalene, rather than glucose, was used as the cometabolite carbon source. Transcriptome analysis revealed significant differential expression of PAH-catabolic genes and ATP-binding cassette transporter-related genes under naphthalene stress. The enhanced degradation of PAHs could be attributed to the augmentation of the PAH metabolic pathway and membrane transportation, facilitating the transfer of PAHs to bacteria. These findings underscore the effectiveness ofP. aeruginosaas a PAH degrader and provide molecular insights into enhancing PAH degradation.
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ACS Applied Materials & Interfaces
·
2025
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IF 8.2
Yujun Chen,Mao Xu,Jieyi Pan,Yuan Liao,Jintong Na,Pengyu Li,Yingying Sun,Shihui Yu,Yongxiang Zhao,Haiyan Hu
Pseudomonas aeruginosa (P. aeruginosa) infections are increasingly challenging due to their propensity to form biofilms and low outer membrane permeability, especially in chronically infected patients with thick mucus. P. aeruginosa exhibits multiple drug resistance mechanisms, making it one of the most significant global public health threats. In this study, we found that moxifloxacin (MXC) and antibacterial peptides (ε-poly-l-lysine, ε-PLL) exhibited a synergistic effect against multidrug-resistant P. aeruginosa (MDR-P. aeruginosa). MXC was combined with ε-PLL to prepare lipase-responsive nanoparticles (MCIP/(PEG–PCL)/PLL NPs) with a weakly negative charge. The weakly negatively charged MCIP/(PEG–PCL)/PLL NPs demonstrated remarkable mucus and biofilm penetration capabilities, thereby overcoming one of the adaptive drug resistance mechanisms. MCIP/(PEG–PCL)/PLL NPs improved the outer and inner membrane permeability and inhibited the expression of the efflux pump MexAB-OprM gene in MDR-P. aeruginosa, thereby overcoming mechanisms of both intrinsic and acquired drug resistance. Meanwhile, the nanoparticles demonstrated an ability to reduce repeated infections with MDR-P. aeruginosa. Additionally, the bacterial burden in the lungs of mice treated with MCIP/(PEG–PCL)/PLL NPs was significantly lower than that in the MXC group, resulting in a 99% clearance rate. Notably, MCIP/(PEG–PCL)/PLL NPs showed no toxicity toward BEAS-2B cells or RAW 267.4 cells, nor did they adversely affect pulmonary function or major organs. This study demonstrated the potential of the nanodrug delivery system composed of the antibiotic moxifloxacin and the antibacterial peptide ε-PLL in addressing the clinical challenges of treating chronic pulmonary infections caused by MDR-P. aeruginosa.
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Environmental Technology & Innovation
·
2024
·
IF 6.7
Fenglin Zhang,Jinming Gu,Yibing Wang,Qing Qiu,Yue Yu,Xuejian Sun,Menghan Chang,Dandan Zhou,Hongliang Huo,Kejian Tian
Bisphenol A (BPA) poses multiple risks to human health, and it has become a critical environmental pollution issue. The advantage of bacterial degradation of BPA has become more prominent, and enhancement methods have become increasingly diverse. Using quorum sensing to regulate functional bacteria and genes to enhance the bacterial degradation efficiency of BPA serves as a highly promising new approach. This study investigated the effect of adding exogenous N-hexanoyl-L-homoserine lactone (C6-HSL) on the degradation of BPA by Pseudomonas asiatica P1 (strain P1) and analyzed the regulatory mechanism of the signaling molecules. The results showed that exogenous homoserine lactone signaling molecules (AHLs) could enhance the BPA degradation efficiency of strain P1, with 10 μM C6-HSL demonstrating the most significant enhancement, increasing the degradation rate by 12.4 % compared with the control group and producing products such as benzoic acid and succinic acid. In terms of the regulatory mechanism, C6-HSL preferentially induced a 5.18-fold upregulation in the expression of the AHL receptor-encoding gene LasR. Subsequently, LasR regulates the upregulation of hub genes such as bvgS and aruS , ultimately achieving an upregulation of BPA degradation genes through hierarchical regulation. This led to an enhancement in the initial degradation step of BPA and the activation of the benzoic acid degradation pathway. This study analyzed the potential of C6-HSL to enhance microbial BPA degradation and, for the first time, clarified the mechanism by which C6-HSL enhanced bacterial BPA degradation efficiency through hierarchical regulation.
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