Nanoscale
·
2018
·Vol. 10
·期 26
·12535-12542
·
IF 7.233
Peng-Fei Guo,Dan-Dan Zhang,Zhi-Yong Guo,Xi-Ming Wang,Meng-Meng Wang,Ming-Li Chen,Jian-Hua Wang
A novel organic–inorganic hybrid was prepared by anchoring (3-aminopropyl)triethoxysilane (APTES) on the surface of monolayer titanate nanosheets and subsequent modification with hydrophilic polyethylene glycol (PEG). The PEGylated hydrophilic monolayer titanate nanosheets were abbreviated as PEG–APTES–TiNSs, and they exhibit a lateral dimension of dozens of nanometers and a thickness of ca. 1.9 nm. PEGylation of the titanate nanosheets significantly improved their selectivity toward the adsorption of glycoproteins through strong hydrophilic interaction, providing an adsorption capacity of 2540.9 mg g−1 for immunoglobulin G (IgG). The retained IgG is readily collected at a recovery rate of 83.4% with 0.5% (m/v) ammonium hydroxide (NH4OH) as the stripping reagent. PEG–APTES–TiNSs are applied for the selective adsorption of IgG from human serum, which is further confirmed by SDS-PAGE assay.
研究领域:
材料科学
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生物化学
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纳米技术
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生物医学工程
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产品关联
DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
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2021
·
IF 4.098
Yujiao Wang,Xiaoyan Cui,Haixiang Gao,Runhua Lu,Wenfeng Zhou
The fluorescent organic nanoparticles (FONs)-based sensor has been attracting great attention in recent years. There are still big challenges in the preparation and application of FONs-based sensor. In this study, a FONs-based sensor was designed and developed through facile hydrothermal process using 3-perylenecarboxaldehyde (PlCA) as the fluorophore and L-methionine (Met) as the recognition site for mercury ions. According to the experimental results, the fluorescence intensity of the as-prepared PlCA-M would decrease when adding Hg 2+ and the mechanism was extrapolated to be photoinduced electron transfer inducing by specific coordination interaction. The acquired PlCA-M−based sensor was used to monitor Hg 2+ in several real samples (environmental water, tea, and apple) with the limit of detection being 60 nM. Remarkably, a visual detection device based on FONs , SDS-PAAG (sodium dodecyl sulfate polyacrylamide gel) @PlCA-M was firstly constructed and successfully used to Hg 2+ semi-quantitation by naked eyes. In addition, the acquired FONs was applied into imaging tool for security information detection and identified as solid-state luminescent material for the first time.
研究领域:
材料科学
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分析化学
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环境科学
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纳米技术
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产品关联
DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
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2024
·
IF 4.3
Rongrong Zhang,Zhen Yan,Zhaodan Xue,Wan Xu,Min Qiao,Liping Ding,Yu Fang
Fluorescent aggregates and ensembles have been widely applied in fabrication of fluorescent sensors due to their capacity of encapsulating fluorophores and modulating their photophysical properties. In the present work, fluorescent ensembles based on anionic surfactant SDS assemblies and perylene derivatives (PBIs) were particularly constructed. Three newly synthesized neutral PBI derivatives with different structures, PO, PC1 and PC2, were used for the purpose to evaluate probe structure influence on constructing fluorescent ensembles. The one with hydrophilic side chains, PO, experienced distinct photophysical modulation effect by SDS assemblies. The ensemble based on PO@SDS assemblies displayed effective fluorescence variation to antibiotic aminoglycosides (AGs). To improve cross-reactivity and discrimination capability of ensembles, a second probe, coumarin, was introduced into PO@SDS assemblies. The resultant ternary sensor, CM-PO@SDS, exhibited good qualitative and quantitative detection capabilities, and achieved differentiation of eight AGs and mixed AG samples both in aqueous solution and actual biological fluid, like human serum. Sensing mechanism studies revealed that hydrogen bonding, electrostatic and hydrophobic interactions are involved in the sensing process. This surfactant-based fluorescent ensemble provides a simple and feasible method for assessing AGs levels. Meanwhile, this work may provide some insights to design reasonable probes for constructing effective single-system based discriminative fluorescent amphiphilic sensors.
研究领域:
分析化学
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药学
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材料科学
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生物技术
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DOI
高影响因子
Functional Materials Letters
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2017
·
IF 1.234
Lei Hu,Chunfu Lin,Changhao Wang,Chao Yang,Jianbao Li,Yongjun Chen,Shiwei Lin
TiNb 2 O 7 nanorods have been successfully fabricated by a sol–gel method with a sodium dodecyl surfate (SDS) surfactant. X-ray diffraction indicates that the TiNb 2 O 7 nanorods have a Ti 2 Nb 1 0 O 2 9 -type crystal structure. Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) results show that the nanorods have an average diameter of ∼ 100 nm and an average length of ∼ 300 nm. As a result of such nanosizing effect, this new material exhibits advanced electrochemical performances in terms of specific capacity, rate capability and cyclic stability. At 0.1 C, it delivers a large first-cycle discharge/charge capacity of 337/279 mAh g − 1 . Its capacities remain 248, 233, 214, 182, 154 and 122 mAh g − 1 at 0.5, 1, 2, 5, 10 and 20 C, respectively. After 100 cycles, its capacity at 10 C remains 140 mAh g − 1 with large capacity retention of 91.0%.
研究领域:
材料科学
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电化学
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纳米技术
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电池技术
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DOI
高影响因子
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
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2025
·
IF 3
Yilin Huang,Xingli Huo,Huijun Liu,Danni Li,Zongning Yin
Lysine succinylation is a major post-translational modification affecting diverse proteins, and its excessive occurrence can lead to protein misfolding and aggregation—hallmarks of various proteinopathies, such as amyloid-β and Tau tangle formation in Alzheimer's disease and islet amyloid polypeptide aggregation in type 2 diabetes. Here, we investigated the inhibitory effects of bile acid metabolites (deoxycholic acid, glycocholic acid, and taurocholic acid sodium) and natural polyphenols (anthocyanin and salidroside) on succinylation and succinylation-induced amyloid aggregation. Succinylation levels were evaluated using the ninhydrin assay before and after treatment, and aggregation behavior and structural alterations were characterized by SDS-PAGE, inverted fluorescence microscopy, and intrinsic fluorescence spectroscopy. Additionally, fluorescence quenching and molecular docking were used to explore the underlying mechanisms. All five small molecules significantly reduced succinylation in a concentration-dependent manner (p < 0.05). Among bile acids, taurocholic acid sodium exhibited the strongest suppression of aggregation (40.99 %), followed by glycocholic acid (28.32 %) and deoxycholic acid (27.94 %). Anthocyanin showed greater inhibition (33.81 %) than salidroside (26.54 %) (all p < 0.05). The results suggest that these small molecules inhibit succinylation-induced aggregation potentially by interacting with proteins and altering their conformations, thereby preventing excessive succinylation at lysine residues. This study provides new insight into the interplay between bile acid metabolism and protein homeostasis and highlights the therapeutic potential of natural compounds in preventing protein aggregation-related diseases.
研究领域:
生物化学
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分子生物学
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神经退行性疾病
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代谢
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药理学
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结构生物学
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF PHARMACEUTICS
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2024
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IF 5.3
Jianlu Qu,Li Wang,Chao liang Jia,Shule Zhang,Cunhao Li,Wei Wu,Wenlong Li
Andrographolide (AG) is a diterpene lactone with significant anti-inflammatory and antitumor activities. However, the poor water solubility limits its clinical application. An andrographolide-salicylic acid (AG-SLA) nano-cocrystal delivery system was rapidly developed using hummer acoustic resonance (HAR) technology in this research. The formulation of the AG-SLA nano-cocrystal suspension and the process parameters for HAR technology were optimized in a high-throughput manner, with SDS-Tween 80 as the optimal composite stabilizer. Nano-cocrystal suspension of AG-SLA with an average particle size of 190 nm were successfully prepared, and then the optimal formulation were tenfold scaled up. Freeze-drying was adopted to solidify the nano-cocrystal and improve its stability. Various analytical techniques were used to characterize the particle size and solid state of the nano-cocrystals. The high-energy input from the HAR instrument induced partial amorphization of the nano-cocrystals, as confirmed by PXRD and DSC analyses. Saturation solubility experiments demonstrated that the solubility in pH 1.2 hydrochloric acid buffer and pH 6.8 phosphate buffer increased by 5.74 times and 6.82 times, respectively, compared to raw AG. In vitro dissolution tests indicated that the cumulative release over 120 min in pH 1.2 hydrochloric acid buffer and pH 6.8 phosphate buffer increased by 1.60 times and 1.88 times, respectively, compared to raw AG.
研究领域:
药学
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纳米技术
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药物递送系统
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分析化学
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产品关联
DOI
PubMed
高影响因子
BMC BIOTECHNOLOGY
·
2019
·Vol. 19
·期 1
·1-11
·
IF 2.303
Zhang Ge,Li Shugui,Xu Yingbo,Wang Juan,Wang Fan,Xin Yuhua,Shen Zhong,Zhang Haibo,Ma Ming,Liu Haobao
Background Tobacco stalk (TS), a major agricultural waste abundant in pectin, has resulted in concerns about the need for its reuse. The nicotine in TS is considered a chemical that is to\xic and hazardous to the environment. Results In this study, Bacillus tequilensis CAS-MEI-2-33 was isolated from cigar wrappers to produce alkaline pectinase using TS. Subsequently, the medium and fermentation conditions for the production of pectinase by B . tequilensis CAS-MEI-2-33 were optimized. The optimal fermentation period, pH of the initial fermentation medium, concentration of TS, and inoculum amount for B . tequilensis CAS-MEI-2-33 were 40 h, 40 g/L, 7.0, and 3%, respectively. Under optimal conditions, the pectinase activity was 1370 U/mL. Then, the enzymatic properties, such as the optimum pH, reaction temperature, temperature stability, and effects of metal ions, were studied. The optimal pH was determined to be 10.0, indicating that the enzyme was an alkaline pectinase. The optimal temperature was 40 °C, and pectinase activity was stable at 40 °C. The Ag + metal ions were shown to remarkably promote enzyme activity. The pectinase was partly purified by ammonium sulfate precipitation, ion exchange chromatography, and Sephacryl S-100 chromatography. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and LC-MS/MS analyses were utilized to analyze the pectinase. Conclusions This study provided a new alkaline pectinase candidate and a new strategy for the use of TS.
研究领域:
生物技术
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酶学
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环境科学
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DOI
PubMed
高影响因子
ACS Biomaterials Science & Engineering
·
2019
·
IF 4.511
Jundong Wang,Huiyuan Guan,Qiang Han,Siyuan Tan,Qionglin Liang,Mingyu Ding
In this work, Yb3+-immobilized hydrophilic phytic-acid-coated magnetic nanocomposites were prepared through a facile route and used to selectively separatrf bovine hemoglobin. Hydrophilic phytic acid (PA) was coated onto the magnetic Fe3O4-PEI via electrostatic interactions, followed by finally chelating with Yb3+ ions, which could produce specific protein binding sites at room temperature in water, and complex instrumentation was not necessary. The performance of as-prepared hybrids (Fe3O4-PEI-PA-Yb3+) was assessed by selectively isolating bovine hemoglobin (BHb). The obtained maximum binding capacity was 347.3 mg g–1. The retained BHb could be eluted under simple elution via using 0.1 M of Na2CO3, giving a recovery of 83%. Moreover, the generation of nanocomposites was demonstrated. In addition, the PA and PEI could improve the hydrophilicity of nanoparticles and further reduce the nonspecific adsorption. Therefore, such nanocomposites were successfully employed to selectively bind and separate BHb from bovine serum as verified by SDS-PAGE and MALDI-TOF MS analysis, providing a new perspective for the isolation of heme proteins in proteomics.
研究领域:
纳米技术
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蛋白质组学
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材料科学
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分析化学
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产品关联
DOI
PubMed
高影响因子
FOOD HYDROCOLLOIDS
·
2020
·
IF 7.053
Lin Yu,Chunhong Xiong,Jianke Li,Wenxiang Luo,Hui Xue,Rui Li,Yonggang Tu,Yan Zhao
In this study, changes in the gel properties, microstructure, and molecular interaction force during the formation of duck egg white (EW) gel induced by ethanol were investigated. Results showed that the viscosity of the EW increased with increasing ethanol concentration and treatment time. The hardness and cohesiveness of the formed gel increased first with prolonged treatment time, then decreased slightly, and finally almost kept stable. The increase in the centrifugal water holding capacity and transformation of immobile and free water into bound water was related to the increasingly compact structure of the gel network. The force that maintained the gel network was mainly ionic bond (~45%) and disulfide bonds (~45%). The hydrogen bond and hydrophobic interaction were extremely low. SDS-PAGE results showed that the ethanol-treated EW proteins were degraded to some extent, but the bands did not significantly change with time. The negative charge content and surface hydrophobicity of the protein increased continuously. The secondary structure of the EW gel showed that the mutual transformation of β -sheet, random coil, α -helix, and β -turn resulted in the good texture characteristics of the gel. The ethanol treatment promoted the disulfide bond cross-linking of the duck EW proteins, and the network structure of the EW gel became increasingly compact, resulting in a series of changes in the water retention and binding water content of the gel. Protein aggregation also occurred, which enhanced the hardness, springiness, and cohesiveness of the duck EW gel.
研究领域:
食品科学
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蛋白质化学
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生物物理学
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DOI
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
·
2023
·
IF 8.6
Zhe Zhai,Xiao Wang,Yan Huang,Hao Yang,Yuchao Zhao
Positively charged nanofiltration (NF) membrane has shown great potential for the removal of heavy metal ions from contaminated water. In order to fabricate positively charged membranes, polyethyleneimine (PEI) is widely used considering the existence of abundant amine groups in this branched macromolecule. However, the PEI-based NF membranes often suffer from low permeance due to the highly dense polyamide film. In this study, a surfactant-plasticizer-synergy (SPS) assisted fabrication strategy is proposed to fabricate PEI-based NF membrane with high performance. The surfactant sodium dodecyl sulfate (SDS) and nontoxic plasticizer acetyl tributyl citrate (ATBC) are added into the aqueous phase and organic phase respectively to regulate the interfacial polymerization (IP) reaction between PEI and terephthaloyl chloride (TPC). As a result of the enhanced miscibility of the two phases at the presence of SDS, the diffusion of PEI monomer is promoted and this contributes to a positively charged membrane surface, which effectively improves the rejections of NF membrane towards positive ions. On the other hand, the plasticizer ATBC softens the polyamide film as confirmed by the reduced Young’s modulus. This could enhance the flexibility of polymer chains and promote the transportation of water molecule. Compared with the nascent PEI-TPC membrane, the permeance of PEI/SDS + TPC/ATBC experiences a dramatic promotion from 2.5 L∙m −2 ∙h −1 ∙bar −1 to 9.7 L∙m −2 ∙h −1 ∙bar −1 and the MgCl 2 rejection also increases from 95.9% to 97.8%. More importantly, the rejections of PEI/SDS + TPC/ATBC towards various heavy metal ions are all above 90.0% and they also follow the order: Mn 2+ (97.6%) > Ni 2+ (96.8%) > Cu 2+ (95.7%) > Cd 2+ (91.0%). This SPS assisted IP reaction may provide a simple and low-cost strategy for the fabrication of high-performance TFC membranes for diverse applications in the future.
研究领域:
环境工程
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膜科学
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水处理
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材料科学
✓ 已验证
产品关联
DOI
高影响因子
ENVIRONMENTAL SCIENCE & TECHNOLOGY
·
2021
·
IF 9.028
Dejun Feng,Yuanmiaoliang Chen,Zhangxin Wang,Shihong Lin
Although membrane distillation (MD) has been identified as a promising technology to treat hypersaline wastewaters, its practical applications face two prominent challenges: membrane wetting and fouling. Herein, we report a facile and scalable approach for fabricating a Janus MD membrane comprising a dense polyvinyl alcohol (PVA) surface layer and a hydrophobic polyvinylidene fluoride (PVDF) membrane substrate. By testing the Janus membrane in direct contact MD experiments using feeds containing a sodium dodecyl sulfate (SDS) surfactant or/and mineral oil, we demonstrated that the dense Janus membrane can simultaneously resist wetting and fouling. This method represents the simplest approach to date for fabricating MD membranes with simultaneous wetting and fouling resistance. Importantly, we also unveil the mechanism of wetting resistance by measuring the breakthrough pressure and surfactant permeation (through the PVA layer) and found that wetting resistance imparted by a dense hydrophilic layer is attributable to capillary pressure. This new insight will potentially change the paradigm of fabricating wetting-resistant membranes and enable robust applications of MD and other membrane contactor processes facing challenges of pore wetting or/and membrane fouling.
研究领域:
化学工程
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材料科学
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环境工程
✓ 已验证
DOI
高影响因子
Innovative Food Science & Emerging Technologies
·
2020
·
IF 4.477
Xiaohong He,Jun Chen,Xuemei He,Zhi Feng,Changhong Li,Wei Liu,Taotao Dai,Chengmei Liu
Modification in solubility and structure of pea protein by industry-scale microfluidization (ISM) at varying pressure (30, 60, 90 and 120 MPa) was investigated. ISM treatment significantly enhanced the solubility and turbidity of pea protein, and solubility was increased by 3.78 folds at 120 MPa. Meanwhile, particle size was reduced, and specific surface area was increased with the increase of pressure. Thick block-like structure was remarkably destructed, which was gradually changed to regular thin fragments. After ISM treatment, bathochromic shift and the increased intensity of intrinsic fluorescence were observed for pea protein. Moreover, surface hydrophobicity and free sulfhydryl groups contents were heightened, indicating protein molecular unfolding/denaturation. SDS-PAGE analysis showed the breaking of disulfide bonds , the degradation of protein fractions into small molecular peptides and the transformation of insoluble protein molecules or aggregates into soluble particles. These findings suggested that ISM treatment would be a potential and effective technique to improve solubility of pea protein, and it would be of great significance to develop pea protein-based food systems. Industrial relevance Poor solubility of pea protein limited its utilities in food industries. Physical modification has attracted much attention owing to no involve of exogenous substances. One of the greatest challenges of the food industry is to find the feasible physical processing technology which could achieve the aim of industrially improving the solubility of commercial pea protein. An industry-scale microfludizer (ISM) recently designed in our laboratory overcame the drawbacks of small microchannel, low processing capacity and easily been blocked of traditional microfluidizers, which was an industrial scale microfluidizer device. The present research provided information that ISM was a potential and useful industrial technique to improve solubility of pea protein, and pea protein with the enhanced solubility may be beneficial to develop health and nutritional products based on plant protein .
研究领域:
食品科学
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蛋白质工程
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生物技术
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物理化学
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）