APPLIED SURFACE SCIENCE
·
2019
·
IF 5.155
Tao Hu,Hanwen Wang,Lijing Zhang,Shengyang Tao
The Surface-enhanced Raman scattering (SERS) effect on the surface of metal conductors can significantly improve the sensitivity of molecular detection. Therefore, it is widely used in the field of chemical sensors. The nanostructure of the metal surface strongly influences the strength of the SERS by adsorption and surface electric field. Herein, we combine microfluidic technology with electroless silver plating to produce hollow microspheres with different wall thicknesses. The microspheres have a diameter of 400 μm and a spherical shell thickness of 40–100 μm. The spherical shell has a porous structure formed by phase separation, on which nanosilver particles can be deposited. The silver-polymer hybrid microspheres can adsorb dyes in aqueous solution and produce significant SERS effects, and the Raman signal enhancement factors can be up to 7.11 × 10 11 . Computational simulations and experimental results show that the Raman enhancement effect is related to the distance between the silver particles on the surface and the hollow structure of the microspheres.
研究领域:
材料科学
·
化学传感器
·
纳米技术
✓ 已验证
产品关联
DOI
高影响因子
APPLIED OPTICS
·
2019
·Vol. 58
·期 17
·4720-4725
·
IF 1.973
Haiyan Zhang,Yuan Yuan,Sheng Yan,Kai Lou,Yibo Gao,Shuxia Wang,Yingzhou Huang
Surface-enhanced Raman scattering (SERS) has powerful capabilities in the field of environmental analysis and biological diagnostics because of its instinctive properties of high sensitivity and label-free detection. However, the fabrication of SERS substrate requires complicated processes and expensive equipment. This paper proposes a simple method approach to synthesize a 3D SERS substrate via electroless galvanic replacement reaction inside a microfluidic channel. Copper microparticles are assembled in a microfluidic channel, and silver nitrate solution is pumped into the channel to in situ produce the silver nanoparticles (Ag NPs) on the surface of copper microparticles. Because the copper particles occupy the channel by stack, the 3D Cu@AgNP SERS substrate can be obtained. The probing molecule (methylene blue) was utilized to investigate with the limit of detection (1×10−7 M). The biological molecules (urea and melamine) have been used to demonstrate its benefits in medical applications, and cancer cell detection has been implemented to demonstrate its benefits in cell biology. In addition, the device can filter and wash cells, forming a simple and fast filter. Our work on this simple fabrication method of active SERS substrate has great value for medical and biological applications.
研究领域:
分析化学
·
生物医学工程
·
环境科学
✓ 已验证
DOI
PubMed
高影响因子
PHYSICAL CHEMISTRY CHEMICAL PHYSICS
·
2024
·Vol. 26
·期 22
·16287-16295
·
IF 2.9
Jinqiao Lu,Fen Yang,Zhen Wang,Jie Huang,Shangzhong Jin,Pei Liang
A phenomenon known as plasmon resonance constitutes a unique optical effect that can induce an enhancement in localized electromagnetic fields, resulting in a substantial increase in the electromagnetic field intensity surrounding metallic nanostructures. In this work, the coupling effect of excitation of surface plasmon polaritons and local surface plasmons in nanoparticles is deeply studied under the background of nanoparticles/one-dimension grating composite structures through grating matching. By employing finite-difference time-domain simulations as our methodological approach, we discern gratings with a periodicity of 1.5 μm support surface plasmon bound states between the gratings. Furthermore, the modulation of SPs along the vertical sidewalls of the grating due to standing wave effects exhibits oscillatory behavior with varying grating heights. Experimental results obtained from the nanoparticle/grating composite SERS substrate validate theoretical predictions, demonstrating higher enhanced Raman signals at 633 nm compared to 532 nm. Remarkably, this structure exhibits good performance, with R6G detection sensitivity down to concentrations as low as 10−10 M and mapping achieving a relative standard deviation of 7.79%, underscoring its uniformity and capability of electromagnetic field enhancement.
研究领域:
光学
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材料科学
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分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Small
·
2015
·Vol. 11
·期 18
·2200-2208
·
IF 8.368
Dian Li,Yuting Zhang,Ruimin Li,Jia Guo,Changchun Wang,Chuanbing Tang
Circulating tumor cells (CTCs) captured from blood fluid represent recurrent cancers and metastatic lesions to monitor the situation of cancers. We develop surface-enhanced Raman scattering (SERS)-coding microsphere suspension chip as a new strategy for fast and efficient capture, recovery, and detection of targeting cancer cells. Using HeLa cells as model CTCs, we first utilize folate as a recognition molecule to be immobilized in magnetic composite microspheres for capturing HeLa cells and attaining high capturing efficacy (up to 95%). After capturing cells, the composite microsphere, which utilizes a disulfide bond as crosslinker in the polymer shell and as a spacer for linking folate, can recycle 90% cells within 20 min eluted by glutathion solution. Taking advantage of the SERS with fingerprint features, we characterize captured/recovered cells with the unique signal of report-molecule 4-aminothiophenol through introducing the SERS-coding microsphere suspension chip to CTCs. Finally, the exploratory experiment of sieving cells shows that the magnetic composite microspheres can selectively capture the HeLa cells from samples of mixed cells, indicating that these magnetic composite microspheres have potential in real blood samples for capturing CTCs.
研究领域:
生物医学工程
·
癌症诊断
·
纳米技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Materials Research Express
·
2016
·Vol. 3
·期 7
·075013
·
IF 0.968
Chunyuan Song,Yuhan Wei,Bingtao Da,Haiting Zhang,Xing Cong,Boyue Yang,Yanjun Yang,Lianhui Wang
Surface-enhanced Raman scattering (SERS) has recently received considerable attention as an ultrasensitive analytic technique. However, its wide application is limited by lack of excellent SERS-active substrates. In this work a SERS substrate with arrayed monolayer films of porous gold nanoparticles is prepared on a solid substrate by a facile, in situ and one-step growth approach. Specifically, the solid substrate was coated with a layer of dense positive charges first by layer-by-layer assembly, followed by patterned a PDMS film with arrayed wells on the substrate. Then the growth solution including chlorauric acid, cetyltrimethylammonium chloride, and ascorbic acid in a certain proportion was transferred into the wells for in situ and one-step growth of porous gold nanoparticles on the substrate. The growth time, feed ratio of the reagents, and repeat times of the in situ growth were studied systematically to obtain optimal parameters for preparing an optimal SERS substrate. The as-prepared optimal SERS substrate not only has good SERS performance with the enhancement factor up to ∼1.10 × 106, but also shows good uniformity and stability. The SERS substrate was further utilized to be ultrasensitive SERS-based chemical sensors for ppb-level detection of highly toxic dyfonate. The preliminary result indicates that the as-prepared SERS substrate has good SERS performance and shows a number of great potential applications in SERS-based sensors.
研究领域:
材料科学
·
分析化学
✓ 已验证
DOI
高影响因子
Nano Research
·
2014
·Vol. 7
·期 8
·1177-1187
·
IF 6.963
Huang Zhulin,Meng Guowen,Huang Qing,Chen Bin,Zhou Fei,Hu Xiaoye,Qian Yiwu,Tang Haibin,Han Fangming,Chu Zhaoqin
Surface-enhanced Raman spectroscopy (SERS) is a fast analytical technique for trace chemicals; however, it requires the active SERS-substrates to adsorb analytes, thus limiting target species to those with the desired affinity for substrates. Here we present networked polyacrylic acid sodium salt (PAAS) film entrapped Ag-nanocubes (denoted as Ag-nanocubes@PAAS) as an effective SERS-substrate for analytes with and without high affinity. Once the analyte aqueous solution is cast on the dry Ag-nanocubes@PAAS substrate, the bibulous PAAS becomes swollen forcing the Ag-nanocubes loose, while the analytes diffuse in the interstices among the Ag-nanocubes. When dried, the PAAS shrinks and pulls the Ag-nanocubes back to their previous aggregated state, while the PAAS network “detains” the analytes in the small gaps between the Ag-nanocubes for SERS detection. The strategy has been proven effective for not only singleanalytes but also multi-analytes without strong affinity for Ag, showing its potential in SERS-based simultaneous multi-analyte detection of both adsorbable and non-adsorbable pollutants in the environment.
研究领域:
材料科学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICA CHIMICA ACTA
·
2025
·
IF 6
Ruiying Lin,Zhangying Jiang,Jinyong Lin,Feifei Tong,Yangmin Wu,Jinquan Hong,Sufang Qiu,Qiong Wu
Background In recent years, significant progress has been made in the treatment of nasopharyngeal carcinoma (NPC). The application of immunotherapy, especially the use of Programmed Cell Death Protein 1 inhibitors, has demonstrated excellent therapeutic effects. However, inconsistent immunotherapy outcomes due to individual differences in patients remain a major challenge, making the search for specific biomarkers to screen for the population who may benefit from immunotherapy a priority. Surface-enhanced Raman spectroscopy (SERS) has shown great potential as a highly sensitive and specific optical analytical tool for identifying and monitoring tumor-related markers in NPC. Results In this study, plasma samples from Nasopharyngeal cancer patients before and after immunotherapy, along with those from healthy volunteers, were tested using label-free SERS combined with plasmapheresis. Especially, the components with varying molecular weight sizes were analyzed via the separation process, thereby preventing the potential loss of diagnostic information that could result from competitive adsorption. Subsequently, a robust machine learning algorithm based on principal component analysis and linear discriminant analysis (PCA-LDA) was used to extract features from plasma SERS data and establish an effective discriminant model. The results showed that in the upper plasma layer, the most optimal discrimination was found between normal patients and those post-treatment, with sensitivity and specificity of 88.5% and 92.3%, respectively. In the lower plasma layer, the most optimal discrimination was found between the pre-treatment and post-treatment patients, with sensitivity and specificity of 84.6% and 80.8%, respectively. Significance and novelty Plasmapheresis can reveal latent diagnostic information in the plasma and holds promise for treating NPC, the screening of the population who may benefit from immunotherapy, and the postoperative evaluation of immunotherapy. Further exploration of the feasibility of using SERS to detect tumor markers in the population who may benefit from immunotherapy for NPC will help improve the therapeutic efficacy, optimize clinical practice, and promote the development of individualized treatment strategies.
研究领域:
肿瘤学
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医学诊断
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生物信息学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2020
·Vol. 412
·期 13
·3063-3071
·
IF 3.637
Li Hongmei,Yue Xiaxia,Gao Ningning,Tang Jun,Lv Xiaoyi,Hou Junwei
Clopidol is one of the most widely used anti-coccidiosis drugs. Its residues in poultry products and the environment pose a serious threat to human health. In this work, microwave-assisted synthesis of magnetic ionic liquid/gold nanoparticles (MIL-Au NPs) as the SERS substrates were first designed for sensitive and reliable determination of clopidol residue in egg samples. The experiment shows that MIL(1-methyl-3-hexyl imidazole ferric tetrachloride ([C 6 mim]FeCl 4 )) and microwave play a key role in the dispersion and morphology of Au NPs. Under the optimal conditions, the as-prepared MIL-Au NPs were applied to the SERS detection of clopidol in methanol and egg solution and its detection limits can be as low as to 0.5 μg/kg (equal to 0.5 ppb) in both solutions. The standard curves with regression coefficients of 0.9298 and 0.93496 were constructed in the linear range of 100–1000 ppb and 0.5–50 ppb for clopidol in egg solutions. Moreover, satisfactory recoveries (97.5–103.2%) were obtained for egg samples. The developed SERS method provides a way for quantitation of clopidol and can be applied for the convenient, reliable, and highly sensitive detection of antibiotic residues in food and environment, which has great potential in food safety and biological monitoring.
Graphical abstract
研究领域:
分析化学
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食品安全
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF MATERIALS SCIENCE
·
2022
·Vol. 57
·期 35
·16965-16973
·
IF 4.682
Wang Tao,Lu Yang,Xu Lin,Chen Zheng Jian
Surface-enhanced Raman scattering (SERS) has drawn attention to broad applications. Here, we investigate the SERS property of the π-conjugated poly(3-hexylthiophene-2,5-diyl) (P3HT) for molecule detection. The crystal structure of π-conjugated P3HT thin films is of the great significance to its SERS property. Our results show that the optimized crystal structure can significantly improve its SERS property. The SERS substrate has an excellent signal reproducibility (RSD < 10%). In addition, the SERS substrate still remains a good Raman enhancement after one year and shows excellent long-term stability compared with those of other types of SERS substrate. The result of our theory calculation shows that significant π-stacking of the probe molecules with the thiophene π-cores molecules plays an important role in the large SERS enhancement by the charge transfer mechanism. The increase in the crystallinity of the P3HT film can improve the charge transfer of the substrate molecules and the methylene blue (MB) molecules via more effective chemisorption of the probe molecules with the substrate molecules. The study contributed to the rational design of the organic semiconductor polymer SERS substrate with high sensitivity, selectivity, and long-term stability. Graphical abstract
研究领域:
材料科学
·
光谱学
·
有机化学
✓ 已验证
DOI
高影响因子
APPLIED SURFACE SCIENCE
·
2018
·
IF 4.439
Chuhong Zhu,Xiaoye Hu,Xiujuan Wang
Surface-enhanced Raman scattering (SERS) is a promising analytical technique with molecular “fingerprint” characteristic and high sensitivity. To achieve high sensitivity, analytes should be located in or around the so-called SERS “hot spots” that are normally formed in sub-10 nm gaps between the neighboring plasmonic nanostructures. Ordinary SERS substrates generally show low sensitivity to the molecules that have weak affinity to noble-metal surfaces. Here, we fabricate an effective SERS system that can concentrate and adsorb organic pollutants in water for sensitive SERS detection. Such a SERS platform is composed of a thin film of Ag nanocubes/graphene oxide hybrid covered on a small Cu foil, and the Cu foil is placed on a hydrophobic teflon surface. The Ag nanocubes/graphene oxide hybrid film is synthesized by growth of graphene oxide on a Cu foil using electrophoresis and subsequent assembly of Ag nanocubes on the graphene oxide in ethanol via van der Waals interactions. Organic pollutant molecules can be concentrated on the hybrid film by adding a droplet of aqueous analyte solution on the hybrid film and evaporating. Due to the hydrophobic nature of teflon surface, the droplet will shrink onto the hybrid film and realize the concentration of analyte molecules on a SERS platform. There exists large delocalized π-electron system in the graphene oxide, so the Ag nanocubes/graphene oxide hybrid can effectively adsorb a large number of analyte molecules. As there are plenty of nano-sized gaps between the Ag nanocubes that are densely attached on the graphene oxide sheets, high density SERS hot spots can be formed in the gaps. As a result, the Ag nanocubes/graphene oxide hybrid has shown high SERS activity with an enhancement factor of 3.9 × 10 8 and good signal uniformity (relative standard deviation ∼12%). It is further demonstrated that the hybrid SERS substrates have exhibited high SERS sensitivity to organic pollutants such as 4-chlorobiphenyl and methyl parathion, showing promising applicability in many fields as diverse as chemical analysis, environmental monitoring, and food safety inspection.
研究领域:
分析化学
·
环境科学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
APPLIED SURFACE SCIENCE
·
2022
·
IF 7.392
Yue Mao,Borong Yu,Huijiao Zhang,Yuanyuan Ma,Fangwei Han,Binbin Zhou,Lili Yang,Zhangang Han
The work reported on the preparation of expanded graphite (EG) coupled with Ag nanoparticles for quick water contaminant adsorption, followed by trace molecules sensing using SERS technique. The surfactant-free composites of Ag/EG were obtained by a simple and controllable “mix-and-heat” mechanochemical method. Most of the analytes were found to be adsorbed within 15 min without any additional physical or chemical activation and its adsorption behavior at room temperature fitted pseudo-second-order kinetic models. As SERS enhanced substrate, less than 10 ppb of crystal violet (CV) and methylene blue (MB) could be determined via Ag/EG. What’s more, the Ag/EG substrate could adapt to strong acid and alkali environment and could be stored for as long as 120 days. Therefore, the composites Ag/EG could be used as a reliable SERS active substrate. Moreover, the substrate could be reused at least four times. In addition, ‘adsorption-filtration’ was conducted through a homemade filter and the filter membrane was employed as substrate support instead of silicon wafers. Such results reveal that Ag/EG as a solid SERS substrate has great potential value for practical applications. And the study provides a new strategy for the development of high-performance sensing of the target analyte in the future.
研究领域:
材料科学
·
分析化学
✓ 已验证
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2021
·
IF 7.46
Rongyang Liu,Li Jiang,Zizhen Yu,Xufeng Jing,Xiu Liang,Dan Wang,Bo Yang,Chengxing Lu,Wei Zhou,Shangzhong Jin
Recent years, two-dimensional transition metal carbonitrides, MXene, have emerged as SERS substrates due to their unique optical and electrical properties. In order to combine the advantages of metal nanoparticles and MXene for SERS substrates, this paper prepared an MXene (Ti 3 C 2 T x ) -Ag nanoparticles (NPs) hybrid biosensor through in-situ electrostatic self-assembly method, in which the citrate-coated Ag NPs are loaded on the negatively surface of Ti 3 C 2 T x flakes through positively charged PDDA polymer. The characterizations show that a large number of Ag NPs uniformly adsorb on both side of Ti 3 C 2 T x nanosheets. The prepared platform exhibits excellent SERS performance, long-term stability and good uniformity by detecting 4-MBA molecules. The electromagnetic field distribution was simulated to theoretically show the Raman enhancement mechanism of this platform. In order to explore the possible application of the sensor in clinical diagnosis and monitoring of biological environment, the platform was used for biomolecule detection. The detection limit of adenine molecules can be as low as 10 −8 M. For dopamine molecules with concentration ranging from 5 × 10 -6 M to 5 × 10 −8 M, the SERS results show a linear relationship between peak intensity and concentration of dopamine molecules. The biosensor was applied for serum detection. The results suggest that MXene-PDDA-Ag NPs hybrid platform could be used as a sensitive and uniform biosensor for label-free quantitative detection of bio-molecular based on SERS method, which could be applied in the field of biotechnology.
研究领域:
材料科学
·
生物技术
·
分析化学
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）