Frontiers in Oncology
·
2021
·
IF 6.244
Li Weinan,Zhang Xiaoyu,Nan Yang,Jia Li,Sun Jialin,Zhang Lina,Wang Yanhong
pH-responsive and CD44 receptor-mediated targeted nanoparticles for eliminating cancer stem cells (CSCs) were developed based on complexes of PEG-poly(β-amino ester) (PEG-PBAE) micelles (PPM) coated with hyaluronic acid (HA) (HA-coated PPM complex, or HPPMc). Thioridazine (Thz) was loaded into HPPMc with a decent drug loading content. The release results of the drug in vitro showed that Thz was released from the HPPMc, which was stimulated by both the acidic pH and specific enzymes. Cytotoxicity studies on mammospheres (MS) revealed that the toxicity potential of Thz-loaded HPPMc (Thz–HPPMc) at pH 5.5 was better than drug solutions. Compared with that at pH 7.4, a higher cellular uptake of a coumarin-6 (C6)-labeled complex at pH 5.5 was observed, which demonstrated that complexes were efficiently taken up in MS. Meanwhile, free HA competitively inhibited the cellular uptake of HPPMc, which revealed that the uptake mechanism was CD44 receptor-mediated endocytosis. Within the acidic endolysosomal environment, the protonation of PBAE facilitated the escape of the complex from the lysosome and releases the drug. The results of in vivo distribution studies and tumor suppression experiments showed that HPMMc could stay in the tumor site of BALB/c nude mice for a longer period of time, and Thz–HPPMc could significantly improve the tumor-suppressing effect. All these results demonstrated the great potential of the multifunctional nanoparticle system for eliminating CSCs.
研究领域:
药学
·
肿瘤学
·
生物材料学
✓ 已验证
DOI
PubMed
高影响因子
Materials Express
·
2021
·Vol. 11
·期 2
·263-270
·
IF 0
Kong Ruize,Li Kunlin,Cao Yu,Wang Hongshu,Han Shen,Zhang Yayong,Hou Zongliu,Liu Jie,Gong Kunmei,Jiang Lihong
Cardiovascular diseases are serious and have become the main cause of death worldwide. Cardiomyocytes (CMs) derived from human induced pluripotent stem cells (hiPSCs) have been widely used in cardiovascular disease modeling, heart development research, drug screening and potential cell
therapy. Efficient production of CMs from hiPSCs is crucial for its’ basic research and clinical translation. The three-dimensional (3D) suspension culture system is preferred in CMs culture, as it can achieve large-scale production and obtain more mature phenotype, but a robust stem
cell culture platform remains unsolved. We previously established a scalable PSC culture platform (termed AIC) that supports PSC passaging at the single-cell level under 3D suspension conditions. However, the efficiency of hiPSCs derived from the AIC system directed differentiation into CMs
remains unclear. The research aims to explore the differentiation efficiency of hiPSCs derived from the AIC system.
研究领域:
干细胞生物学
·
心血管研究
·
生物技术
·
再生医学
✓ 已验证
DOI
ADVANCED FUNCTIONAL MATERIALS
·
2020
·Vol. 30
·期 49
·2006169
·
IF 16.836
Honghui Wu,Xinchi Jiang,Yaosheng Li,Yi Zhou,Tianyuan Zhang,Pei Zhi,Jianqing Gao
To date, only few a types of nanomedicines have been made available for clinical use even in the most common liposomal delivery systems, due to significant constraints, including poor targeting of specific cells, hindered penetration, and accelerated clearance. Various intricate designs have been generated and show improvements, while also bringing new problems. Biological cells, possessing advantages like low immunogenicity, long circulation time, receptors integration, and innate targeting capability. Herein, liposomes are innovatively functionalized with a stem cell membrane through a facile approach. The biomimetic vesicles achieve controlled release, exhibit better stability, longer circulation time, and targeted delivery. Loading with curcumin leads to enhanced survival rate of ischemic stroke mice with a single injection (from ≈30% to over 90%). The versatility of the method is verified by differently charged liposomes modification and erythrocyte membrane derived vesicles preparation. Overall, the cell membrane derived biomimetic vesicles achieve targeted delivery and versatility prepared by a facile approach, as well as effortless preparation.
研究领域:
药学
·
生物医学工程
·
纳米技术
✓ 已验证
产品关联
DOI
高影响因子
ACS Applied Materials & Interfaces
·
2019
·
IF 8.456
Fei Jia,Suya Lin,Xuzhao He,Jiamin Zhang,Shuxian Shen,Zhiying Wang,Bolin Tang,Cheng Li,Yongjun Wu,Lingqing Dong,Kui Cheng,Wenjian Weng
The surface electric potential of biomaterials has been extensively proven to play a critical role in stem cells’ fate. However, there are ambiguous reports on the relation of stem cells’ osteogenic capacity to surface potential characteristics (potential polarity and intensity). To address this, we adopted a surface with a wide potential range and both positive/negative polarity in a comprehensive view to get insight into surface potential-regulating cellular osteogenic differentiation. TbxDy1–xFe2 alloy/poly(vinylidene fluoride-trifluoroethylene) magnetoelectric films were prepared, and the film could provide controllable surface potential characteristics with positive or negative polarity and potential (ϕME) intensity variation from 0 to ±120 mV as well as keep the surface chemical composition and microstructure unchanged. Cell culture results showed that osteogenic differentiation of mesenchymal stem cells on both positive and negative potential films was obviously upregulated when the /ϕME/ intensities were set from 0–55 mV. Differently, the highest upregulated osteogenic differentiation on the positive potential films corresponded to the /ϕME/ intensity from 35–55 mV and was better than that on the negative potential films whereas the highest on the negative potential films corresponded to the /ϕME/ intensity from 0–35 mV and was better than that on the positive potential films. This fact could illustrate why previous reports appeared ambiguously; i.e., the comparative result in osteogenic differentiation between the positive and negative potential films strongly depends on the selection of surface potential intensity. On the basis of assaying of the exposed functional sites (RGD and PHSRN) of the adsorbed fibronectin (FN) and the expression of cellular integrin α5 and β1 subunits, the difference in the behavior between the positive and negative potential films was attributed to the distinct conformation of adsorbed fibronectin (FN) and the opposite changing trend with /ϕME/ for the two films, which triggers the osteogenesis-related FAK/ERK signaling pathway to a different extent. This study could provide new cognition for the in-depth understanding of the regulation mechanism underlying surface potential characteristics in cell behaviors.
研究领域:
生物材料学
·
细胞生物学
·
组织工程学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF MATERIALS SCIENCE & TECHNOLOGY
·
2016
·
IF 2.267
Jie Hou,Huichang Gao,Yingjun Wang,Delin Cheng,Xiaodong Cao
Modifying substrates through mineralization is a popular way to improve the osteogenic performance. Screening of the best mineralization characteristics on specific substrates for stem cells is meaningful but not fully studied. In this paper, poly(lactic-co-glycolic acid)/hydroxyapatite (PLGA/HA, PH) microsphere scaffolds with superficial pores were fabricated by a low-temperature fusion method. After the mineralization in the 5× stimulated body fluid (SBF) for 0, 7, 12 and 24 h, four mineralized scaffolds (MPH-0, MPH-7, MPH-12 and MPH-24) with different apatite topographies were obtained. It was found that the surface of MPH-7 was evenly decorated with abundant micro-pores, MPH-12 with dense and plain apatite layer, and MPH-24 with small spherical bumps. The responses of mouse bone mesenchymal stem cells (mBMSCs) to the four scaffolds were further studied. The results showed that MPH-7 and MPH-24 had more obvious effects on mBMSCs attachment, proliferation and differentiation than MPH-0 and MPH-12. This work indicated that to obtain the maximum improvement, the mineralization characteristics had to be carefully chosen. This was noteworthy in the chemical modification of surfaces to form the functionalized scaffolds for bone repair.
研究领域:
生物材料
·
组织工程
·
干细胞研究
·
材料科学
·
生物医学工程
✓ 已验证
产品关联
DOI
高影响因子
CELLULAR PHYSIOLOGY AND BIOCHEMISTRY
·
2016
·Vol. 39
·期 4
·1391-1403
·
IF 4.652
Zhang Lei,Jiang Guoliang,Zhao Xueling,Gong Yuekun
Background/Aims: We investigated the role of dimethyloxalylglycine (DMOG) in bone marrow mesenchymal stem cell (BMSC) osteogenesis mediated by RhoA/ROCK. Methods: BMSCs were cultured with and without DMOG and/or Y-27632 (ROCK1 inhibitor). Cell proliferation, alkaline phosphatase (ALP) levels, and calcium deposits were determined. The expression of Runx2, OSX, p-cofilin, RhoA, and GTP-bound RhoA was determined by real-time RT-PCR and Western blot. Rho-associated coiled-coil-containing protein kinase (ROCK) activity was determined by measuring the phosphorylation of myosin-binding subunit of myosin phosphatase using an ELISA kit. Actin morphology was observed by immunofluorescence. Results: After 24 h, DMOG (0.5 mM) increased the expression of GTP-bound RhoA (+141%, P < 0.001) and enhanced ROCK activity (315%, P < 0.001). DMOG (0.5 mM) enhanced ALP levels after 3, 7, and 21 days of osteogenic induction (all P < 0.001) and strengthened calcium deposition (P < 0.001). In addition, compared with controls, DMOG (0.5 mM) increased the mRNA levels of osteogenesis genes RUNX2 and OSX (all P < 0.001). Furthermore, compared with controls, DMOG increased the expression of p-cofilin (+57%, P < 0.001), which resulted in rearrangement of actin filaments. All these effects were abolished, at least in part, by Y-27632. Conclusion: DMOG promotes BMSC osteogenic differentiation via activation of RhoA/ROCK, suggesting clues for future therapies using BMSCs
研究领域:
干细胞生物学
·
骨骼生物学
·
细胞信号传导
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
·
2016
·
IF 2.371
Zhijian Zhang,Zhengnan Li,Wenwen Deng,Qinghua He,Qiang Wang,Wentao Shi,Qian Chen,Wenjing Yang,Myron Spector,Aihua Gong,Jiangnan Yu,Ximing Xu
Many neurological diseases are closely associated with demyelination caused by pathological changes of oligodendrocytes . Although intrinsic remyelination occurs after injury, the regeneration efficiency of myelinating oligodendrocytes remains to be improved. Herein, we reported an initiative finding of employing a valuable cell source, namely neural crest-derived ectoderm mesenchymal stem cells (EMSCs), for promoting oligodendrocyte differentiation and maturation by co-culturing oligodendrocyte precursor cells (OPCs) with the EMSCs. The results demonstrated that the OPCs/EMSCs co-culture could remarkably increase the number and length of oligodendrocyte processes in comparison with the mono-cultured OPCs and non-contact OPCs/EMSCs transwell culture. Furthermore, the inhibition experiments revealed that the EMSCs-produced soluble factor Sonic hedgehog, gap junction protein connexin 43 and extracellular matrix molecule laminin accounted for the promoted OPC differentiation since inhibiting the function of anyone of the three proteins led to substantial retraction of processes and detachment of oligodendrocytes. Altogether, OPCs/EMSCs co-culture system could be a paradigmatic approach for promoting differentiation and maturation of oligodendrocytes, and EMSCs will be a promising cell source for the treatment of neurological diseases caused by oligodendrocyte death and demyelination.
研究领域:
神经科学
·
干细胞生物学
·
细胞神经科学
·
分子生物学
✓ 已验证
DOI
PubMed
高影响因子
Biomass Conversion and Biorefinery
·
2022
·
IF 4.05
Li Xiao,Zhao Qingqing,Han Ming,Zhang Ke,Guo Zhongya,Li Bin,Wang Wei,Wei Xuhe,Liang Miao
In the present study, the effects of pretreatments with different solvents (hydrochloric acid, sodium hydroxide, and ethanol) on the physicochemical structure and pyrolysis behavior of tobacco stem (TS) were investigated. Elemental compositions analysis showed that acid washing increased the carbon content and exhibited a high removal percentage for the inorganic species. SEM, BET, and FTIR analysis showed that the pretreatments exerted different effect on the surface and microstructure. Furthermore, the pyrolysis characteristics analysis indicated that solvent pretreatment increased the initial pyrolysis temperature due to the removal of unstable volatile components, especially for the acid and alkali washing samples. Meanwhile, hydrochloric acid pretreatment increased the maximum pyrolysis rate temperature of cellulose decomposition stage and decreased the char residues due to the large removal of inorganic species, while alkali treatment produced the opposite result. Pyrolysis kinetic analysis based on Coats-Redfern method showed that F1.5 chemical reaction model can be used to describe the pyrolysis stages of TS samples and a decreased activation energy was observed during the decomposition of cellulose stage for the acid and alkali washed samples. The released gaseous products during the pyrolysis process were also monitored by using TG-FTIR technique, and the gaseous release behavior was different from each other.
研究领域:
能源与燃料
·
化学工程
✓ 已验证
产品关联
DOI
高影响因子
Royal Society Open Science
·
2023
·Vol. 10
·期 1
·
IF 3.5
Fei He,Chendong Yang,Haoye Liu,Jizeng Wang
A thorough understanding of the changes in mechanical property behind intracellular biophysical and biochemical processes during differentiation of human mesenchymal stem cells (hMSCs) is helpful to direct and enhance the commitment of cells to a particular lineage. In this study, displacement creep of the mesenchymal cell lineages (osteogenic, chondrogenic and adipogenic hMSCs) were determined by using atomic force microscopy, which was then used to determine their mechanical properties. We found that at any stages of differentiation, the mesenchymal cell lineages are linear viscoelastic materials and well matched with a simple power-law creep compliance. In addition, the viscoelasticity of mesenchymal cell lineages showed different trends during differentiation. The adipogenic hMSCs showed continuous softening at all stages. The osteogenic and chondrogenic hMSCs only continuously soften and become more fluid-like in the early stage of differentiation, and get stiffened and less fluid-like in the later stage. These findings will help more accurately imitate cellular biomechanics in the microenvironment, and provided an important reference in the biophysics biomimetic design of stem cell differentiation.
研究领域:
细胞生物学
·
生物物理学
·
干细胞研究
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Nano Research
·
2023
·Vol. 16
·期 5
·7413-7421
·
IF 9.9
Jiang Xin-Chi,Wu Hong-Hui,Zhang Tianyuan,Dong Yun-Fei,Li Yao-Sheng,Huang Ting,Tian An-Hao,Chen Peng-Xiang,Lin Xian-Ming,Huang Ying-Zhi,Liu Chong,Zhang Xiang-Nan,Chen Zhong,Tabata Yasuhiko,Gao Jian-Qing
Exosomes make a significant contribution during stem cell-based therapy due to the abundant contents. Accumulating evidence implies exosomes can act as potential biological nano agents. We herein propose hypoxic preconditioning for neural stem cells (NSCs) that could produce hypoxic exosomes for efficient treatment of ischemic stroke. Hypoxic preconditioning on NSCs significantly altered the miRNAs encapsulated in exosomes. Notably, hypoxic exosomes could target the injured brain to regulate the microenvironment to inhibit neuroinflammation and promote blood-brain barrier permeability recovery. Additionally, the autologous NSCs in Nestin-CreER mice could be activated by hypoxic exosomes to facilitate nerve regeneration. After hypoxic preconditioning, exosomes further exerted therapeutic effects on both survival (25%) and behavioral outcomes in ischemic stroke mice. Overall, hypoxic preconditioning NSCs can produce effective nano agent and may represent a promising strategy for clinical neurorestorative therapy.
研究领域:
神经科学
·
干细胞生物学
·
分子生物学
·
临床治疗
✓ 已验证
产品关联
DOI
高影响因子
ADVANCED FUNCTIONAL MATERIALS
·
2023
·
IF 19
Hanbai Wu,Shuo Shi,Huiqun Zhou,Chuanwei Zhi,Shuo Meng,Weng-Fu Io,Yang Ming,Yuchao Wang,Leqi Lei,Bin Fei,Jianhua Hao,Jinlian Hu
Smart electroactive materials that can dynamically regulate stem cell fate without external stimuli have fascinated increasing attention. Meanwhile, noninvasive electrical stimulation has emerged as a less restrictive approach, generating considerable interest in its potential biomedical applications. The intricate interplay between cells and materials within complex microenvironments includes encompassing substrate response, ion exchange, and membrane potential alterations. However, the mechanisms by which smart materials influence stem cells have yet to be fully elucidated. Herein, electrospinning technology is utilized to fabricate bone-mimicking microenvironments comprising disordered and highly oriented polyvinylidene fluoride (PVDF) nanofibers. The aligned annealed PVDF (AA) and random annealed PVDF (RA) membranes present high fractions of β-phase. By comparing the osteogenic ability, calcium activity, and F-actin distribution of bone marrow-derived mesenchymal stem cells (BMSCs) cultured with these PVDF nanofibers, it is proposed that the stem cells autonomously regulate their differentiation by remodeling the cytoskeleton on the electrospun membranes. Electrical stimulation, more adhesion area, and active calcium influx support the greater osteogenesis of BMSCs on RA than AA. This mechanism can provide a basic theory for the design and preparation of bone tissue engineering scaffolds and contribute to the further study of cells and microenvironments.
研究领域:
生物医学工程
·
组织工程
·
细胞生物学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
ADVANCED MATERIALS
·
2023
·
IF 29.4
Ye Feng,Chao Gao,Dazhi Xie,Lu Liu,Bin Chen,Suyi Liu,Haihong Yang,Zhan Gao,Daniela A. Wilson,Yingfeng Tu,Fei Peng
Neural stem cells (NSCs), with the capability of self-renewal, differentiation, and environment modulation, are considered promising for stroke, brain injury therapy, and neuron regeneration. Activation of endogenous NSCs, is attracting increasing research enthusiasm, which avoids immune rejection and ethical issues of exogenous cell transplantation. Yet, how to induce directed growth and differentiation in situ remain a major challenge. In this study, a pure water-driven Ni–Zn micromotor via a self-established electric–chemical field is proposed. The micromotors can be magnetically guided and precisely approach target NSCs. Through the electric–chemical field, bioelectrical signal exchange and communication with endogenous NSCs are allowed, thus allowing for regulated proliferation and directed neuron differentiation in vivo. Therefore, the Ni–Zn micromotor provides a platform for controlling cell fate via a self-established electrochemical field and targeted activation of endogenous NSCs.
研究领域:
生物医学工程
·
神经科学
·
细胞生物学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）