MICROCHEMICAL JOURNAL
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2022
·
IF 5.304
Fangbin Xiao,Ju Liu,Xiaoyan Feng,Guoyang Xie,Wanqing Zhang,Hengyi Xu
Staphylococcus aureus ( S. aureus ) is a common foodborne pathogen, which could cause serious bacteremia and infective endocarditis. Establishing a rapid and low-cost method to capture and detect S. aureus in food samples is necessary. In this study, we have developed a method on the basis of polyethyleneimine-functionalized magnetic nanoparticles (MNP-PEI) and recombinase aided amplification (RAA) for rapid enrichment and analysis of S. aureus in milk. The capture efficiency of MNP-PEI on multiple strains of bacteria and influencing factors were studied. The limits of detection (LOD) of the MNP-PEI-RAA strategy were 2.9 × 10 1 CFU·mL −1 in phosphate-buffered saline (PBS) and 2.9 × 10 2 CFU·mL −1 in milk. The entire enrichment and detection processes could be completed within 60 min. The detection strategy based on MNP-PEI and RAA had low cost, short detection time, and high sensitivity. In addition, the broad-spectrum of bacteria captured by MNP-PEI was important for simultaneous analysis of a variety of bacteria, which provided an effective tool for evaluating the microbiological safety of food samples.
研究领域:
食品安全
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微生物学
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纳米技术
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生物技术
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产品关联
DOI
高影响因子
MOLECULAR PHARMACEUTICS
·
2021
·
IF 4.939
Zhe Wang,Xingyun Liu,Yanwen Duan,Yong Huang
Skin and soft tissue infections require effective and sustained topical administration. Platensimycin (PTM) is a natural drug lead that targets bacterial fatty acid synthases and has a great potential to treat infections caused by methicillin-resistant Staphylococcus aureus (MRSA). To facilitate the use of PTM against local MRSA infections, we prepared polyacrylamide hydrogels containing polyamidoamine (PAMAM)/PTM nanoparticles (NP-gel(PTM)) for the controlled release of PTM. NP-gel(PTM) can continuously inhibit the growth of MRSA and its biofilm formation in simulated drug flow models in vitro. In situ implantation of NP-gel(PTM) could treat MRSA-infected subcutaneous soft tissues without toxicity. For MRSA-infected skin wounds, NP-gel(PTM) not only showed strong anti-MRSA activity but also accelerated more wound healing than the widely used antibiotic mupirocin. Collectively, PTM is expected to be used in this safe and effective NP-gel delivery platform for the treatment of local infections, which might help to alleviate the current antibiotic resistance crisis.
研究领域:
药理学
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生物材料
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感染病学
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产品关联
DOI
PubMed
高影响因子
Materials Today Bio
·
2023
·
IF 8.2
Ming-Chen Sun,Ying-Fang Chen,Di Liu,Xiao-Ling Xu,Yu-Chan You,Wei Lu,Yun-Juan Shi,Ming-Yang Ren,Yi-Bin Fan,Yong-Zhong Du,Xiao-Hua Tao
The widespread utilization of mupirocin to treat methicillin-resistant Staphylococcus aureus (MRSA)-caused infectious diseases has led to the emergence of mupirocin-resistant Staphylococcus aureus (MuRSA), posing a serious global medical threat. In order to counteract MuRSA, we develop a d -α-tocopherol polyethylene glycol 1000 succinate (TPGS) modified mupirocin and silver complex (TPGS/Mup-Ag) to combat MuRSA. The surfactivity of TPGS endows Mup-Ag with a homogeneous and small particle size (∼16 nm), which significantly enhances bacterial internalization. Silver ions are released from the mupirocin-Ag complex (Mup-Ag) to exert a synergistic antibacterial activity with mupirocin. Results manifest that our strategy reduces the concentration of mupirocin that induces 50% bacterial death from about 1000 μmol/mL to about 16 μmol/mL. In vitro bacterial infection model suggests that TPGS/Mup-Ag can not only eliminate both intracellular and inhibit bacterial adhesion, but also living cells are not affected. Results of in vivo experiments demonstrate that TPGS/Mup-Ag can effectively inhibit the progression of skin infection and accelerate wound healing, as well as alleviate systemic inflammation in both the subcutaneous infection model and the wound infection model. Furthermore, this study may contribute to the development of therapeutic agents for antibiotic-resistant bacteria and offer ideas for silver-based bactericides.
研究领域:
药理学
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微生物学
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传染病
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纳米技术
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药物递送系统
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产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2023
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IF 8.4
Beibei Lu,Jialei Bai,Jingyang Zhang,Huijing Shen,Minglin Wang,Yujing Lian,Zhixian Gao,Yuan Peng
Colorimetric sensing materials have a rising demand in detection applications, especially in the field of naked-eye detection. Herein, a novel iridescent polymeric film that can respond to different Staphylococcus aureus enterotoxin B (SEB) concentrations in the visible range is reported. This film is fabricated by polymerization of methacrylic acid (MAA) and ethylene dimethacrylate (EGDMA) owing an inverse opal array (IOA) structure, and gold nanoparticles (AuNPs) are enchased on the polymer skeleton. This composite structure facilitates the film to respond to SEB at a LOD of 4.29 fg mL −1 , making it the best performing colorimetric material to date. Cryo-Scanning Electron Microscopy and 3D AFM were used to observe the morphology change of the iridescent polymeric film after SEB binding. This film possesses great advantages for ultrasensitive visual detection of real samples. In addition, the iridescent polymeric film has the potential to become a universal sensing material, realizing the ultrasensitive visual detection of hazardous substances without equipment in the field by replacing the recognition element.
研究领域:
材料科学
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分析化学
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生物传感
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纳米技术
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产品关联
DOI
高影响因子
BIOORGANIC CHEMISTRY
·
2023
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IF 5.1
Zhishang Zhang,Wenqing Li,Haorui Wu,Zhuangfeng Liu,Huaiyi Huang
The challenge of antibiotic resistance worldwide has brought an urgent need to explore novel drugs for bacterial infections. Antimicrobial photodynamic therapy has been proven to be a potential antimicrobial modality but is limited by biofilms. In this study, we synthesized three cationic photosensitizers with strong photoinduced antimicrobial and antibiofilm activities toward gram-positive Staphylococcus aureus . The indole–pyridine compounds illustrated multiple type I/II photosensitization and coenzyme NAD(P)H photocatalytic activity upon excitation. A mechanistic study showed that intracellular reactive oxygen generation and NAD(P)H oxidation caused membrane damage, leading to protein/nucleus acid leakage. This research provides insights into the development of novel chemotherapeutics with synergetic photodynamic and photocatalytic reactivity.
研究领域:
药学
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微生物学
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光化学
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抗菌研究
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产品关联
DOI
PubMed
高影响因子
International Journal of Nanomedicine
·
2023
·
IF 8
Xiangjie Fu,Yaqiong Ni,Guanchen Wang,Runda Nie,Yang Wang,Run Yao,Danyang Yan,Mingming Guo,Ning Li
Background Multidrug-resistant staphylococcus aureus infected wounds can lead to nonhealing, systemic infections, and even death. Although advanced dressings are effective in protecting, disinfecting, and maintaining moist microenvironments, they often have limitations such as single functionality, inadequate drug release, poor biosafety, or high rates of drug resistance.Methods Here, a novel wound dressing comprising glycyrrhizic acid (GA) and tryptophan-sorbitol carbon quantum dots (WS-CQDs) was developed, which exhibit synergistic and long-lasting antibacterial and anti-inflammatory effects. We investigated the characterization, mechanical properties, synergistic antibacterial effects, sustained-release properties, and cytotoxicity of GA/WS-CQDs hydrogels in vitro. Additionally, we performed transcriptome sequence analysis to elucidate the antibacterial mechanism. Furthermore, we evaluated the biosafety, anti-inflammatory effects, and wound healing ability of GA/WS-CQDs dressings using an in vivo mouse model of methicillin-resistant staphylococcus aureus (MRSA)-infected wounds.Results The prepared GA/WS-CQDs hydrogels demonstrated superior anti-MRSA effects compared to common antibiotics in vitro. Furthermore, the sustained release of WS-CQDs from GA/WS-CQDs hydrogels lasted for up to 60 h, with a cumulative release of exceeding 90%. The sustained-released WS-CQDs exhibited excellent anti-MRSA effects, with low drug resistance attributed to DNA damage and inhibition of bacterial biofilm formation. Notably, in vivo experiments showed that GA/WS-CQDs dressings reduced the expression of inflammatory factors (TNF-α, IL-1β, and IL-6) and significantly promoted the healing of MRSA-infected wounds with almost no systemic toxicity. Importantly, the dressings did not require replacement during the treatment process.Conclusion These findings emphasize the high suitability of GA/WS-CQDs dressings for MRSA-infected wound healing and their potential for clinical translation.
研究领域:
药学
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材料科学
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生物医学工程
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传染病学
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产品关联
DOI
PubMed
高影响因子
BMC Medicine
·
2025
·Vol. 23
·期 1
·1-14
·
IF 8.3
Cheng Xin,Wang Zerui,Liu Yadong,Ma Liyan,Wang Zhiyi,Qiao Yuxin,Du Wenbin,Guo Shuilong,Chen Dongwei,Su Jianrong
Background Heterogeneous vancomycin-intermediate Staphylococcus aureus (hVISA) is associated with suboptimal glycopeptide treatment outcomes. However, conventional antimicrobial susceptibility testing fails to distinguish hVISA from vancomycin-susceptible Staphylococcus aureus (VSSA), potentially delaying effective therapy. The gold standard population analysis profiling with area under the curve (PAP-AUC) method is labor-intensive and time-consuming, necessitating rapid detection alternatives. Methods This study introduces a microfluidic platform based on fluorescence-activated droplet sorting (FADS) for the rapid detection and isolation of hVISA. Through systematic optimization of key parameters such as droplet generation conditions, bacterial suspension concentration, fluorescence probe selection, fluorescence duration, and vancomycin screening concentration, we established a rapid hVISA screening system. The efficacy of this system was evaluated using the hVISA standard strain Mu3, followed by validation with 15 clinical hVISA isolates. Genomic analysis further elucidated the genetic basis of drug resistance in hVISA strains. Results We developed a high-throughput detection platform for hVISA by integrating a microfluidic system with FADS. Based on a Poisson distribution theoretical model, the bacterial suspension concentration was optimized to 10 6 CFU/mL, achieving a single-bacterium droplet encapsulation rate of approximately 30%. The selected fluorescent probe exhibited detectable fluorescence signals as early as 6 h post-cultivation, with stability maintained for at least 1 week. Drug concentration validation demonstrated that 4 μg/mL vancomycin effectively inhibited susceptible bacteria without interfering with fluorescence detection. This platform successfully isolated resistant subpopulations from the hVISA reference strain Mu3 within 12 h, and its efficacy was further validated using 15 clinical hVISA isolates. Conclusions This study innovatively integrated a microfluidic detection platform and FADS to achieve rapid detection of hVISA. This technology reduced the detection time to just 12 h and significantly improved the enrichment efficiency of drug-resistant bacterial populations.
研究领域:
微生物学
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抗生素耐药性
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微流控技术
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生物医学工程
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传染病学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF MICROBIOLOGICAL METHODS
·
2018
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IF 1.701
Jinjuan Qiao,Xiangying Meng,Yufang Sun,Qian Li,Ronglan Zhao,Yun Zhang,Jiaqi Wang,Zhengjun Yi
Accurate and rapid identification of methicillin-resistant Staphylococcus aureus (MRSA) is of important clinical significance. In this study, a novel aptamer-based fluorometric assay was developed for detection of MRSA in clinical samples by coupling with immunomagnetic separation . The S. aureus cells in clinical specimens were enriched by magnetic separation. Following lysis by staphylococcal lysin , the PBP2a proteins were released from S. aureus cells and detected by the aptamer-based fluorometric assay. Without lengthy period of bacteria cultivation in the traditional susceptibility testing, this test has an overall testing time of only 2 h with the detection limit of 2.63 × 10 3 and 1.38 × 10 3 CFU/mL in PBS and spiked nasal swab, respectively. Since it is simple, rapid and sensitive, this method could be used for the detection of MRSA in various clinical samples.
研究领域:
医学检验
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微生物学
·
临床诊断
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DOI
高影响因子
ANALYST
·
2019
·Vol. 145
·期 1
·165-171
·
IF 4.019
Hui Yang,Xiao Zhao,Hui Wang,Wenfang Deng,Yueming Tan,Ming Ma,Qingji Xie
We report here a facile synthesis of ZnS/CdS heterojunction nanoparticles on an indium–tin oxide (ITO) electrode and their application in the ultrasensitive photoelectrochemical detection of Staphylococcus aureus (S. aureus). The ZnS/CdS/ITO electrode was prepared using one-pot electrodeposition in an acidic solution containing ZnCl2, CdCl2 and Na2S2O3. The optimal ZnS/CdS heterojunction nanoparticles with a Zn/Cd atomic ratio of 1 : 1 showed a high photoelectrochemical response to L-cysteine. L-Cysteine-encapsulated liposome (cysteine@liposome) immunonanocapsules were prepared and used as the labels for photoelectrochemical detection of S. aureus. By coupling cysteine@liposome immunonanocapsule labeling with immunomagnetic separation/enrichment and photoelectrochemical analysis using the ZnS/CdS/ITO electrode, sensitive photoelectrochemical detection of S. aureus was achieved. Under optimal conditions, the linear range for photoelectrochemical detection of S. aureus was from 1 to 4000 CFU mL−1. The proposed method was successfully used for photoelectrochemical detection of S. aureus in milk and juice samples.
研究领域:
生物医学工程
·
微生物学
·
分析化学
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DOI
高影响因子
TALANTA
·
2019
·
IF 4.916
Xuan Wang,Caicheng Long,Xiaoyan Xu,Taiping Qing,Peng Zhang,Bo Feng
Micrococcal nuclease (MNase) is a nonspecific endo-exonuclease that digests single-stranded/double-stranded DNA and RNA. The existence of MNase can serves as an important diagnostic biomarker of Staphylococcus aureus ( S. aureus ) infection. However, most of the substrates in MNase-based sensors are single-stranded DNA, which could also be digested by exonuclease I or S1 nuclease and interfere the MNase detection. In this work, we developed a highly selective fluorescent method for MNase detection using a specific dsDNA and nucleic acid dye SYBR Green I (SGI) as the indicator. After rational design, an AT-rich dsDNA with 3′ protruding termini was screened as the high-specific substrate of MNase assay and efficient enhancer of SGI. The AT-rich dsDNA substrate can resist the digestion of other exonuclease and greatly enhance the fluorescence of SGI. This high-specific substrate-based probe can detect MNase in buffer as well as biological sample with highly selectivity . Moreover, this method was also applied to monitor the MNase secreted by S. aureus . Thus, the proposed MNase-based assay has a strong potential to identify S. aureus in food safety and microbial infection due to its excellent analytical sensitivity and high selectivity.
研究领域:
微生物学
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分子生物学
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医学诊断
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DOI
PubMed
高影响因子
FOOD CONTROL
·
2022
·
IF 6.652
Hui Li,Yan-yan Huang,Keren Agyekumwaa Addo,Yi-gang Yu,Xing-long Xiao
The inhibitory mechanism of CUM on Staphylococcus aureus exotoxins and invasive enzyme, and the effect of CUM on sauced beef were investigated. Treatment with sub-minimum inhibitory concentrations of CUM observed very little decrease in cell density after 24 h. Additionally, CUM reduced hemolytic activity by inhibiting the formation of heptamer, and inhibited the content of enterotoxin in culture medium and sauced beef. The transcription of genes encoding α-hemolysin, staphylococcal enterotoxin A and Panton-Valentine leukocidin were down-regulated after treatment with CUM. Compared with the control group, the addition of CUM caused a significant decrease in S. aureus counts, total volatile base nitrogen, acid value, and thiobarbituric acid reactive substances of sauced beef after 9 d. Furthermore, CUM improved the redness (a*) of sauced beef. These results confirmed that CUM could effectively restrain the synthesis of S. aureus toxin, and the addition of CUM to sauced beef was the potential way to inhibit the growth of food pathogens and maintain quality profiles.
研究领域:
食品科学
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微生物学
·
毒理学
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食品安全
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DOI
高影响因子
Nano Select
·
2022
·Vol. 3
·期 11
·1537-1547
·
IF 0
Wenshuai Liu,Litao Jia,Sichun Zhao,Yue Ma,Siyu Liu,Qian Wang,Xiaoyan Lv,Haiyue Jiang,Xia Liu
Bacterial infection can cause delay in wound healing, and reactive oxygen species (ROS) was accumulated in wounds, which threaten patients’ lives. Therefore, it needs to develop a multifunctional hydrogel for infected wound healing. Here, a multifunctional QAS-CS@GA-GM hydrogel with antibacterial and antioxidant property, established by the self-adaptive covalent bond, is prepared aiming to eradicate bacteria and eliminate ROS. It is observed that QAS-CS@GA-GM hydrogel displays remarkable ability of clearing ROS and killing bacteria in vitro. With its antibacterial and antioxidant activity, QAS-CS@GA-GM hydrogel significantly accelerates the MRSA-infected wound closure and reduces inflammatory, when QAS-CS@GA-GM hydrogel was applied to wound sites. Collectively, the research suggests that a self-adaptive QAS-CS@GA-GM hydrogel is a promising wound dressing as antibacterial and ROS-scavenging dressing for bacterial-infected wound.
研究领域:
生物材料
·
伤口敷料
·
抗氧化
·
抗感染
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DOI
危险品化学品经营许可证（带存储）