Sensors and Actuators Reports
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2025
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IF 7.6
Junting Liang,Dihan Duan,Lubing Sun,Jinming Li,Mengke Wang,Ziyi Chang,Rick F. Thorne,Chuanliang Chen,Demin Duan
Cysteine (Cys) is essential for numerous physiological processes and play a vital role in disease diagnostics, underscoring the necessity for effective and sensitive detection techniques for Cys. In this research, we introduce an ultrasensitive colorimetric approach based on oxidase-like Mn 3 O 4 nanozymes. The nanozymes were synthesized through a straightforward wet chemical method and displayed a flower-like globular morphology, showing remarkable oxidase-like catalytic performance with a low K m value of 0.1896 mM and a high V max of 2.8 × 10⁻⁷ M s⁻¹. By leveraging the outstanding catalytic activities of Mn 3 O 4 nanozymes and optimizing the TMB concentration, we developed a novel and sensitive colorimetric sensor for Cys detection. Experimental results indicated a detection range for Cys between 0.5 and 45 μM, with a lower limit of detection (LOD) of 0.02636 μM. This established method for detecting Cys reveals significant promise for the advancement of effective sensing systems.
研究领域:
生物化学
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分析化学
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纳米技术
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疾病诊断
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DOI
高影响因子
MICROCHIMICA ACTA
·
2021
·Vol. 189
·期 1
·1-7
·
IF 5.833
Xia Chunqiu,Huang Wenying,Kang Xiutang,Chen Pingyun,You Longjie,Guo Liangqia
Mo 5 N 6 nanosheets were synthesized by a nickel-induced growth method and were found to possess peroxidase-like activity in acidic condition and catalase-like activity in weak basic condition. In acidic condition, Mo 5 N 6 nanosheets can catalyze the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) by H 2 O 2 to form a blue color product (TMB OX ). At the co-existence of 4-aminophenol (4-AP), 4-AP can react with H 2 O 2 and TMB OX , resulting in the decrease of TMB OX and the fading of blue color. Therefore, a facile, sensitive colorimetric method for the quantitative detection of 4-AP was developed. The linear range for 4-AP was 1.0 to 80.0 μmol⋅L ‒1 ( R 2 = 0.999), and the detection limit was 0.56 μmol⋅L ‒1 based on 3 σ / k . Resorcinol, aniline, humic acid, and common ions and anions in surface water did not interfere the determination of 4-AP. This colorimetric method was applied to measure the 4-AP in real water sample from Wulong River in Fujian Province of China. The relative standard deviation for the determination of 4-AP was ranged from 0.03 to 1.88%, and the recoveries from spiked samples were ranged between 99.2 and 107.6%. The determination results were consistent with those obtained by HPLC. Graphical abstract
研究领域:
材料科学
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分析化学
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环境科学
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产品关联
DOI
PubMed
高影响因子
Analytical Methods
·
2019
·Vol. 11
·期 31
·4014-4021
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IF 2.378
Qiang Li,Feng Wu,Mao Mao,Xiang Ji,Luyao Wei,Jieying Li,Lan Ma
In this study, water-soluble citrate-capped copper nanoparticles (Cu NPs) were synthesized by a simple and rapid method. We found that these citrate-capped Cu NPs possessed an intrinsic peroxidase-like activity, which could catalyse the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) to generate a light blue product in the presence of hydrogen peroxide (H2O2). It was also found that mercury ions (Hg2+) could enhance the peroxidase-like activity of the citrate-capped Cu NPs, causing the colour to turn bright blue. The colour change was dependent on the concentration of Hg2+. Therefore, a colorimetric method for Hg2+ detection was established with a linear range from 0.050 μM to 10.000 μM and a detection limit of 0.185 Mm. More interestingly, citrate-capped Cu NPs have a characteristic absorption peak at 260 nm, we also found that Hg2+ could cause the absorption peak at 260 nm to change. Therefore, we developed another colorimetric method for Hg2+ detection based on the absorption peak of citrate-capped Cu NPs at 260 nm. This colorimetric method was shown to enable convenient and sensitive quantification of Hg2+ in the concentration range of 0.100 μM to 6.000 μM with a limit of detection of 0.052 μM. In this study, a dual-mode sensor for detection of Hg2+ was constructed, which exhibited good sensitivity and selectivity.
研究领域:
分析化学
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纳米技术
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环境科学
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产品关联
DOI
高影响因子
ANALYTICA CHIMICA ACTA
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2015
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IF 4.513
Chaohui Chen,Ningxing Li,Jingwen Lan,Xinghu Ji,Zhike He
A target-catalyzed hairpin assembly (CHA) and graphene/Au-NPs hybrids-based platform has been developed for the determination of DNA . This new sensor not only avoided any labeling but also reduced the background signal. In the absence of target, the assembly of H1 and H2 couldn't be triggered. The catalytic activity of graphene/Au-NPs hybrids was inhibited by adsorption of H1 and H2, leading to the “inactive” hybrids unable to catalyze the oxidation reaction of 3,3′,5,5′-tetramethylbenzidine (TMB). However, with the addition of target DNA, the target-catalyzed hairpin assembly was initiated and produced plenty of H1–H2 duplex, which had a weak binding affinity with the graphene/Au-NPs. Thus, the protected interface of graphene/Au-NPs hybrids became active and catalyzed the oxidation reaction of TMB accompanied with a colorless to-blue color change. This approach exhibited good sensitivity and specificity for target DNA with a detection limit of 5.74 × 10 −11 M, and realized the assay of target DNA in human serum samples. Besides, this sensor could be further expanded to detect viruses or proteins by adapting the corresponding aptamers, showing great potential in biochemical detections.
研究领域:
生物化学
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纳米技术
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分子生物学
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分析化学
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产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
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2017
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IF 5.401
Xike Tian,Xiang Wang,Chu Dai,Yong Li,Chao Yang,Zhaoxin Zhou,Yanxin Wang
Glucose concentration in human body is a significant indicative for diabetes screening, so it is very significant to develop rapid and sensitive methods for glucose detection. Herein, CoSe 2 /reduced graphene oxide (CoSe 2 /rGO) nanohybrids were synthesized via a simple and convenient hydrothermal method, and also their morphologies and compositions were systematically characterized. The CoSe 2 /rGO nanohybrids were found to possess an intrinsic peroxidase-like activity and can effectively catalyze the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) in the presence of hydrogen peroxide (H 2 O 2 ) accompanied with a blue color change. The catalytic activity of CoSe 2 /rGO nanohybrids is matched with the typical Michaelis–Menten kinetics and highly dependent on reaction time, pH, incubation temperature, and H 2 O 2 concentration. The peroxidase-like CoSe 2 /rGO nanohybrids were successfully applied for the detection of H 2 O 2 and glucose with the detection limit as low as 0.23 μM and 0.553 μM, respectively. Furthermore, some other carbohydrates, such as lactose, sucrose, galactose and fructose have no interferences with the detection of glucose. The detection platforms for glucose developed in the present work not only confirmed that the CoSe 2 /rGO nanohybrids possess intrinsic peroxidase-like activity but also showed great potential applications in varieties of simple, robust, and easy-to-make analytical approaches in the future.
研究领域:
分析化学
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纳米技术
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生物传感器
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糖尿病研究
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产品关联
DOI
高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
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2022
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IF 5.999
Wei Yang,Jianwen Fei,Wei Xu,Hongyan Jiang,Marwan Sakran,Junli Hong,Wanying Zhu,Xuemin Zhou
High plasma urate is closely related to gout, cardiovascular and other diseases. Therefore, monitoring the content of uric acid (UA) in plasma is of great significance for the treatment of gout and the prevention of other related diseases. Herein, a biosensor based on the biomimetic oxidase Fe 3 O 4 nanoparticles (NPs) @MnO 2 nanosheets (Fe 3 O 4 @MnO 2 NS) was constructed for colorimetric determination of UA. MnO 2 NS is an efficient biomimetic oxidase, and we found that the intrinsic oxidase activity of MnO 2 NS doped with Fe 3 O 4 NPs can be significantly enhanced. The chromogenic substrate TMB can be catalyzed by Fe 3 O 4 @MnO 2 NS to generate blue oxidized TMB, and UA can decompose the MnO 2 NS to inhibit the color reaction of TMB selectively, thereby realizing the quantitative detection of UA. In addition, the UA biosensor can perform colorimetric analysis of UA level through three methods: naked eye, smartphone and ultraviolet-visible (UV–vis) spectrophotometer. The linear ranges of UV–vis spectrophotometry and colorimetry with smartphone were 1–70 μM and 200–650 μM, respectively, and the limits of detection (LOD) were 0.27 μM and 21 μM. The analysis results of human plasma samples showed that the method had good selectivity and practicability.
研究领域:
生物传感器
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分析化学
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材料科学
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医学诊断
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产品关联
DOI
PubMed
高影响因子
ANALYST
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2022
·Vol. 147
·期 9
·1815-1823
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IF 5.227
Yue Wang,Cheng Cheng,Ruofei Ma,Zhangrun Xu,Yukihiro Ozaki
Visualization of signaling molecules in single living cells is crucial for understanding cellular metabolism and physiology, which can provide valuable insights into early diagnoses and treatments of diseases. Highly sensitive in situ monitoring of intracellular analytes released from single living cells by virtue of label-free nanosensors is urgently needed, which can avoid interferences from molecular labeling. Here, we proposed an ultrasensitive strategy for in situ imaging of intracellular H2O2 in single living cancer cells by surface-enhanced Raman scattering (SERS) spectroscopy with the utilization of label-free Fe3O4@Ag core–satellite nanoparticles (NPs). The Fe3O4@Ag NPs can efficiently and selectively catalyze the oxidation of the peroxidase substrate 3,3′,5,5′-tetramethylbenzidine (TMB) in the presence of H2O2. Additionally, they exhibit excellent SERS activity that allows for in situ monitoring of intracellular H2O2 in living cells through establishing the correlation between the H2O2 level and the SERS intensity of the catalytic oxidation product of TMB. The H2O2 concentration is revealed through the SERS intensity of oxidized TMB with a good linear response in a wide range from 1 fM to 1 mM. Moreover, the intracellular H2O2 level in live cancer cells and imaging of the distribution of H2O2 inside single cells can be achieved by using such a label-free nanosensor based strategy. Our work demonstrates that the label-free Fe3O4@Ag NP-based SERS imaging and quantification strategy is a promising and powerful approach to assess intracellular H2O2 in living cells and allows us to monitor single-cell signaling molecules with nanoscale resolution.
研究领域:
纳米技术
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生物医学工程
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分析化学
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细胞生物学
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癌症研究
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
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2023
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IF 4.3
Zhangxu Zhou,Yulong Liu,Taocui Yan,Shixin Tu,Hongli Guo,Jin Zhou,Ziqian Ye,Zhilun Zhang,Keyu Li,Pei Zhao,Guowei Zuo,Baoru Han
Breast milk, as the most ideal food for infants, contains the main nutrients to meet the growth and development of infants. Lactose is an essential energy source in breast milk, the concentration of which varies based on a variety of circumstances, including maternal diet and lactation stage. Studies demonstrated that lactose content could affect the development of the brain and gut immunity of infants. We integrated the enzyme cascade reaction with colorimetry , and then established a data-prediction model utilizing multi-point absorbance analysis and a back-propagation (BP) neural network. Lactose underwent an enzymatic cascade to generate H 2 O 2, which oxidized the chromatic substrate 3,3′5,5′-tetramethylbenzidine (TMB). Absorbance at several time intervals and wavelengths was measured. Different from the traditional end-point detection methods and their single input data model, the absorbance data were detected at various time points and then fitted by BP neural network in this research. The detection of lactose in breast milk showed an average recovery rate of 99.14%. Meanwhile, our strategy is time-efficient and required no complex sample preparation processes. The robust nonlinear mapping and high fault tolerance of the BP neural network significantly enhances the stability and precision of the colorimetric analysis . Overall, this approach may be used for low-cost, convenient, and accurate lactose quantification in breast milk.
研究领域:
分析化学
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生物化学
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神经网络
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营养科学
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产品关联
DOI
高影响因子
CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
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2022
·
IF 1.193
Yan WU,Jian-Mei ZHOU,Yue-Shan JIANG,Wen LI,Meng-Jie HE,Yao XIAO,Jin-Yang CHEN
As a major origin of energy for cellular activity and intermediates product of metabolism, glucose plays a significant role in biological activity. Thus, it is relatively vital to explore a sensitive and accurate approach to monitor blood glucose. Herein, a colorimetric biosensor for sensitive, rapid, and reliable monitoring of blood glucose was successfully established. In this sensing system, a nanocomposite of silver nanoparticles (AgNPs) depositing on the MIL-101 (Fe) surface ( [email protected] ) was employed as an excellent peroxidase mimic. Glucose could be effectively catalyzed by glucose oxidase (GOx) to produce hydrogen peroxide (H 2 O 2 ) and gluconic acid. Afterward, the [email protected] nanozyme catalyzed the oxidation of the colorless material TMB into the blue product oxTMB by H 2 O 2 , causing a noticeable change in absorbance at 652 nm. A colorimetric glucose measurement platform was proposed with preferable simplicity and high sensitivity using GOx and [email protected] nanozyme with peroxidase-mimicking activity. The limit of detection of the established biosensor was down to 0.17 µM for H 2 O 2 and 0.23 µM for glucose. More importantly, as a result of the application of this biosensor with human blood samples, glucose levels could be monitored with high accuracy, which indicated that this approach had great potential for diabetes therapy.
研究领域:
纳米技术
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生物传感
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分析化学
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生物医学工程
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产品关联
DOI
高影响因子
Journal of Materials Chemistry C
·
2023
·Vol. 11
·期 38
·13047-13055
·
IF 6.4
Xuemei Zhou,Lingmin Kong,Junkai Hao,Jing Feng,Shuo Sun,Chuanzhen Zhou,Yanmin Liu,Zhengquan Yan,Xiao Zhu,Lei Hu
To strengthen the enzyme-mimetic catalytic activity of the classical transition metal oxide of copper and iron, bimetal Cu–Fe oxide mesoporous nanoparticles (Cu–Fe MNPs) were prepared in one pot under microwave-assisted conditions. The proposed Cu–Fe MNPs possessed a well-distributed mesoporous structure with an average aperture of 3.91 nm and a high BET specific surface area of 197.93 m2 g−1. Their peroxidase-like kinetic parameters (Km/Vmax) toward catalyzing the redox reactions of TMB and H2O2 were 0.013 mM/3.01 × 10−8 M s−1 and 56.58 mM/4.12 × 10−8, respectively. In particular, both toxic o-aminophenol (o-AP) and bioactive glutathione (GSH) could deteriorate their peroxidase-like property with an obvious color change from blue to colorless in the proposed Cu–Fe MNPs-TMB-H2O2 system. Under optimized conditions, Cu–Fe MNPs-TMB-H2O2 showed selective response to o-AP and GSH with quite low detection limits of 1.57 × 10−8 mol L−1 (S/N = 3) and 3.35 × 10−8 mol L−1 (S/N = 3), respectively. When they were applied for the visual monitoring of o-AP and GSH in real aqueous, fruit juice and medicinal samples, the detection recovery (%) was between 96.8–101.4% for o-AP and 97.1–105.8% for GSH. The mechanisms of the synergetic enhancement of the peroxidase-like activity of Cu–Fe MNPs and the selective recognition of o-AP and GSH were investigated in detail.
研究领域:
材料科学
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纳米技术
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分析化学
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环境科学
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产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
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2020
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IF 7.1
Hongbo Li,Mingbin Liu,Weihua Zhao,Jiamei Pu,Jianguo Xu,Suqin Wang,Ruqin Yu
Nucleic acids-sensing methods provide the potential to enable and hasten early disease detection and to rationalize treatments, especially for cancers. In this work, a colorimetric label-free sensing system based on target-triggered split-to-intact remodeling of dual-G-rich duplex probe (Dual-GDP) has been developed for amplified signaling of Pax-5 gene. In this sensing system, the Dual-GDP is splited into one hairpin-structured G-rich probe (HGP) and one duplex-shaped G-rich probe (DGP), which can be re-built to an intact form through target gene triggered accurate base-pairing. Significantly, this unique split-to-intact remodeling behavior allows the occurrence of a well-designed active isothermal amplification that can amplify target response with seven stages to multi-channel accumulate numbers of G-quadruplex transducers. When specifically combined with hemin, the formed G-quadruplex-hemin complexes, also known as DNAzymes, can strongly catalyze the oxidization of TMB to a colored TMB + and robustly reflect the target gene even in complex biological environments. We demonstrate that this method offers simplified operations and improved assay abilities for Pax5 gene analysis. We envision these findings might provide new insights to exploit versatile oligonucleotide probes and nucleic acid-based amplification strategies for diverse bioanalytical applications.
研究领域:
分子生物学
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分析化学
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癌症诊断
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核酸传感
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DOI
高影响因子
MICROCHIMICA ACTA
·
2025
·Vol. 192
·期 10
·1-13
·
IF 5.3
Meng Dongling,Chen Zhiyan,Chen Zhibin,Cen Junrao,Li Jitao,Yang Yaling
Iron-cerium co-doped carbon dots (Fe,Ce-CDs) were synthesized by one-step hydrothermal method using tartaric acid and L-tryptophan as ligands. Fe,Ce-CDs shows excellent peroxidase-like (POD) activity and nitrite (NO 2 − ) can promote the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) to its blue oxidation product (oxTMB) due to the formation of ∙NO 2 free radical. NO 2 − further react with oxTMB to form a yellow color via diazotization resulting in the absorbance Change at 450 nm. By Fe,Ce-CDs + NO 2 − + TMB + H 2 O 2 and oxTMB + NO 2 − systems, a dual-signal colorimetric nanosensor for NO 2 − detection is developed with lower detection limits (LODs) of 0.272 μg/L and 0.019 mg/L, respectively. The proposed methods were applied to the detection of NO 2 − in tobacco samples with good recovery from 91.20 to 110.61%, achieving similar results and indicating their high accuracy and feasibility. Graphical
研究领域:
分析化学
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纳米材料
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环境与食品安全
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传感器与生物传感器
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）