FOOD HYDROCOLLOIDS
·
2020
·
IF 7.053
Xiepeng Mo,Xinling Peng,Xianrui Liang,Sheng Fang,Hujun Xie,Jie Chen,Yuecheng Meng
In this study, natamycin-loaded zein/casein composite nanoparticles (Nata-Z/C NPs) were fabricated and incorporated into gelatin film to improve the film's physicochemical and antifungal properties. The mean particle diameter (MPD) and zeta potential (ZP) values of the Nata-Z/C NPs were characterized by dynamic light scattering (DLS) with 68.5 ± 1.2 nm and −33.1 ± 0.8 mV, respectively. The Nata-Z/C NPs were well dispersed in the gelatin film and improved its mechanical properties, including the tensile strength (from 30.5 ± 3.2 MPa to 51.4 ± 3.0 MPa) and percentage elongation at break (from 0.225 ± 0.087 to 0.685 ± 0.103). The NPs as hydrophobic filler also improved the water barrier properties to 0.335 × 10 −9 g ˑ m/m 2 ˑ Pa ˑ s and water contact angle to 122.55 ± 1.54° of the gelatin film. The possible interactions between the Nata-Z/C NPs and gelatin were studied by differential scanning calorimetry (DSC), Fourier-transform infrared spectroscopy (FTIR), and scanning electron microscopy (SEM) techniques. The sustained release of natamycin from the film was achieved by its encapsulation, and the release kinetics were assessed with the Avrami model. Furthermore, the nanocomposite films exhibited strong antifungal activities against Aspergillus niger , Botrytis cinerea , and Penicillium citrinum . This study revealed that the Nata-Z/C NPs could be used as both nanofillers and functional additives to improve the physicochemical and bacteriostatic properties of gelatin films.
研究领域:
材料科学
·
食品科学
·
纳米技术
·
生物技术
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2016
·
IF 2.857
Hualin Wang,Lilan Hao,Baicheng Niu,Suwei Jiang,Junfeng Cheng,Shaotong Jiang
The proanthocyanidins encapsulated in zein (zein-PA) fibers was via electrospinning technique. The kinetics and antioxidant capacity of PA from zein fibers was investigated by cyclic voltammetry. Circular dichroism was used to investigate the secondary structure change of zein and its influence on the shape of fibers. The addition of PA caused a significant increase in viscosity and made fibers wider. These hydrogen bonds between zein and PA molecules would favor the α-helix change and decrease the β-folds of zein in electrospinning solutions, leading to a round-shaped tendency of fibers and enhancing the thermal properties slightly. Zein-PA fibers showed high encapsulation efficiency close to 100%, and the encapsulated PA retained its antioxidant capacity in fibers. Zein-PA fibers showed a good controlled release toward PA, and the predominant release of PA from fibers was Fickian diffusion, which could be well described by first-order model and Hixson–Crowell model.
研究领域:
食品科学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2021
·
IF 4.952
Yanan Xu,Zihao Wei,Changhu Xue,Qingrong Huang
Recently, the carbodiimide-mediated coupling method has attracted the attention of some scholars in the food field for its safety and high efficiency. In this study, the carbodiimide method was utilized to assemble zein–polyphenol conjugates innovatively. The covalent insertion of polyphenols to zein was confirmed by the increase of molecular weight and the reduction of nucleophilic groups. Chlorogenic acid (CGA) as well as Gallic acid (GA) were selected as polyphenol models, and the total phenolic content of zein–CGA conjugate and zein–GA conjugate reached 71 mg/g and 43 mg/g, individually. Afterwards, the structural and chemical characteristics between zein–polyphenol conjugates and physical mixtures were compared. Scanning electron microscopy (SEM) indicated that zein–polyphenol conjugates formed regularly shaped spheres with small particle sizes. Compared with control zein and physical mixtures, zein–polyphenol conjugates demonstrated better solubility and dispersibility, higher thermal stability as well as lower surface hydrophobicity. In terms of antioxidant capacity, zein–GA conjugate showed higher scavenging abilities of ABTS and DPPH free radicals than zein–CGA conjugate. The present research developed a new approach to fabricating zein–polyphenol conjugates that can be applied in the food industry as functional additives or materials for delivery systems.
研究领域:
食品科学
·
材料科学
·
化学
✓ 已验证
产品关联
DOI
高影响因子
FOOD HYDROCOLLOIDS
·
2022
·
IF 11.504
Yijie Wang,Xiufeng Li,Ting Li,Yang Wang,Jie Jiang,Xuhui Zhang,Jing Huang,Bihua Xia,Ho Cheung Shum,Zhenzhong Yang,Weifu Dong
Designing solid particles with desirable interfacial affinity from plant-based ingredients is a key area in sustainable food production. Here, a chemical approach is introduced to fabricate conjugate particles by combining a water-insoluble plant protein (zein) and a hydrophilic polysaccharide (cellulose) through Schiff-base reaction. Zein assembles and precipitates into anisotropic particles after forming conjugates with cellulose . The wettability of conjugate particles at the interface can be precisely engineered by adjusting the ratio of the hydrophobic and hydrophilic components. The three-phase contact angle of a water droplet sitting on the substrate made of particles can be adjusted in a range from around 130°–40° when they are submerged in corn oil. The interfacial absorption kinetics of conjugate particles shows 2 to 4 times improvement than the particles made from sole material sources. The optimal polymer ratio is found to be 1:1.5 and 1:2 (zein:cellulose) for the corn oil-water system. In association with the enhanced interfacial affinity, emulsions stabilized by conjugate particles exhibit superior stability against droplet coalescence and bulk phase separation when going through the isoelectric point of the protein (around pH 6), high ionic strength, long-term storage, and thermal treatment. It has been found that 0.5 wt% conjugate particles are enough to stabilize emulsions containing more than 60 vt% oil.
研究领域:
食品科学
·
材料科学
·
化学工程
✓ 已验证
产品关联
DOI
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2022
·
IF 6.449
Ximing Zhong,Hongjian Wen,Runxin Zeng,Hongkai Deng,Guofeng Su,Hongjun Zhou,Xinhua Zhou
Excessive use of non-renewable surfactants, low utilization efficiency, and uncontrollable release behavior are the urgent disadvantages in traditional pesticide formulations . In this work, zein was used to modify mesoporous silica as nanocarriers to load avermectin to obtain the nanopesticides. Due to the unique properties of zein, the nanopesticides were responsive to pH and enzyme. The pesticide release at alkaline condition was controlled by the electrostatic repulsion between zein and pesticide, while the controlled release of pesticide in the presence of enzyme was due to the cleavage of peptide bond in zein, and the relevant release mechanisms were also elucidated. Besides, these nanopesticides exhibited a high pesticide loading capacity above 280 mg g −1 , and such nanocarriers reduced the degradation rate of the pesticide against ultraviolet irradiation about 55%. Also, these nanopesticides demonstrated enhanced wetting and adhesion properties on cucumber foliar surfaces, and they show comparable pesticidal efficacy with the pure pesticide. Therefore, this work provides a facile and environmentally friendly strategy to prepare a dual responsive nanopesticide system, and the controlled release performance, high pesticide loading capacity, enhanced photostability, remarkable pesticidal efficacy, and improved wetting and adhesion properties of the resultant nanopesticides make them potential for agricultural applications.
研究领域:
农业科学
·
纳米技术
·
材料科学
·
环境科学
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Long Chen,Wen Xu,Zhongyu Yang,David Julian McClements,Xinwen Peng,Zhenlin Xu,Man Meng,Yidong Zou,Guanxiong Chen,Zhengyu Jin
Three nanoparticles were fabricated for the co-delivery of quercetin and resveratrol. Nanoparticles consisted of a zein and carboxymethyl cellulose assembled using antisolvent precipitation/layer-by-layer deposition method. Nanoparticles contained quercetin in the core and resveratrol in the shell, resveratrol in the core and quercetin in the shell or both quercetin and resveratrol in the core. The particle sizes of nanoparticles were 280.4, 214.8, and 181.8 nm, respectively. Zeta-potential was about −50 mV and PDI was about 0.3. The different positions of polyphenol distribution nanoparticles could reduce the competition between the two polyphenols, the encapsulation rate, loading rate and storage stability reached up to 91.7 %, 5.37 % and 97.1 %, respectively. FT-IR showed that hydrophobic and electrostatic interactions were the main driving forces of nanoparticle assembly. XRD showed that two polyphenols were successfully encapsulated in nanoparticles. TGA showed that distributing the nanoparticles in different layers would enhance thermal stability. TEM and SEM showed that polysaccharides attached to the surface of nanoparticles formed a core-shell structure with uniform particle size. All three nanoparticles could release two polyphenols slowly in simulated gastrointestinal digestion, Korsmeyer-Peppas was the most suitable kinetic release model. Therefore, biopolymer-based nanocarriers can be created to enhance the loading, stability, and bioaccessibility of co-encapsulated nutraceuticals.
研究领域:
食品科学
·
药物递送
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Jianyu Huang,Jiahao Liao,Xiaoqiong Li,Hui Zhao,Hongxia Li,Jian Kuang,Jianqiang Li,Jinbin Guo,Tao Huang,Jinjun Li
In this study, in order to solve the application problems of poor water solubility and low bioavailability of quercetin, we prepared a nano-delivery system with core-shell structure by anti-solvent method, including a hydrophilic shell composed of tea saponin and a hydrophobic core composed of Zein, which was used to improve the delivery efficiency and biological activity of quercetin. Through the optimal experiments, the loading rate and encapsulation rate of nanoparticles reached 89.41 % and 7.94 % respectively. And the water solubility of quercetin is improved by 30.16 times. At the same time, the quercetin acted with Zein through non-covalent interaction and destroyed its spatial network through structural characterization, while tea saponin covered the surface of Zein through electrostatic interaction, making it change into amorphous state. In addition, the addition of tea saponin makes the nanoparticles remain stable under the changes of external environment. During simulating gastrointestinal digestion procedure, ZQTNPs has higher release rate and bioavailability than free quercetin. Importantly, ZQTNPs can overcome the limitations of a single substance through synergy. These results will promote the innovative development of quercetin precision nutrition delivery system.
研究领域:
药学
·
纳米技术
·
食品科学
·
生物技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Food Chemistry-X
·
2024
·
IF 6.5
Dongjing Wu,Wei Wu,Na Zhang,Olugbenga P. Soladoye,Rotimi E. Aluko,Yuhao Zhang,Yu Fu
This study aimed to modify plant protein mixture to improve their functionality and digestibility by limited hydrolysis. Soy protein isolate and corn zein were mixed at the ratio of 5:1 ( w /w), followed by limited hydrolysis using papain from 15 to 30 min. The structural characteristics, in vitro digestibility, and functional properties were evaluated. Also, DPPH radical scavenging activity was determined. The results indicated that the molecular weight of different modified samples was largely reduced by limited hydrolysis, and the proportion of random coil was significantly increased. Furthermore, the solubility, foaming, emulsifying and water-holding capacity of hydrolyzed protein mixture were significantly improved, which were close to those of whey protein isolate. In vitro digestibility after 30-min limited hydrolysis was remarkably elevated. In addition, the hydrolyzed protein mixture exhibited a higher antioxidant activity than those of untreated proteins. Overall, limited hydrolysis of protein mixture led to improved digestibility, functionality and antioxidant activity.
研究领域:
食品科学
·
蛋白质化学
·
酶学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Peifang Chen,Yong Wang,Zizhe Cai,Xuanxuan Lu
Cyclolinopeptides (CLS) are hydrophobic cyclic peptides in flaxseed with multiple bioactive activities. This study developed zein ( Z )-cyclodextrin (CD) binary nanoparticles (NPs) as an oral delivery system for CLS. Z -CD NP had a smaller diameter (D h ) and better encapsulation effect on CLS. Formation of CLS-loaded NPs was driven by hydrogen bonds and electrostatic interactions. Presence of CD improved the thermal, pH and storage stabilities of NPs. Besides, CD prevented premature release of CLS in the stomach and enhanced the bioaccessibility of CLS to a maximum of 86.71 % ± 2.20 %. Lipid-raft-mediated endocytosis was involved in the cell uptake of NPs, where the addition of CD significantly facilitated the uptake of NPs. Z -CD NPs also enhanced absorption and reduced secretion of CLS after digestion. Overall, this study provides a simple approach to enhance the oral delivery efficiency of CLS by modulating Z-based NPs with CD.
研究领域:
食品科学
·
药学
·
纳米技术
·
生物技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Food Measurement and Characterization
·
2024
·Vol. 18
·期 5
·3868-3880
·
IF 2.9
Mayire Abulitifu,Wei Qin,Wang Yingbo,Bai Xi
The citronellol-rich Origanum vulgare essential oil (OEO), which exhibits excellent antioxidant and antimicrobial activities, has limited use in food packaging owing to its high volatility and instability. This problem can be solved using electrospinning. In this study, OEO was incorporated into the zein matrix via emulsion electrospinning. OEO was obtained by hydro-distillation; gas chromatography–mass spectrometry analysis revealed that citronellol (44.34%) was a predominant compound in OEO. Scanning electron microscopy images demonstrated that the fibers had a bead-free and uniform structure with a diameter ranging from 215 ± 36 to 260 ± 72 nm. The X-ray diffractograms indicate the amorphous structure of the zein fibers. Fourier transform infrared absorption spectroscopy revealed an interaction between OEO and zein. The addition of OEO to the zein nanofibers affected its secondary structure. Thermogravimetric analysis showed that the OEO-loaded (OEO/zein) nanofibers exhibited high thermostability and that the water contact angle was enhanced. The encapsulation efficiency and loading capacity were in the range of 71.56–85.8% and 8.88–40.93%, respectively. The antioxidant activity of the OEO/zein nanofibers ranged from 45.40% to 71.50%. The OEO/zein fibers displayed antimicrobial activity against Staphylococcus aureus and Escherichia coli . In conclusion, OEO/zein nanofibers are promising alternatives for active food packaging.
研究领域:
食品科学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY
·
2016
·Vol. 16
·期 9
·9497-9504
·
IF 1.338
Cui Jing,Wang Qing-Qing,Qiu Yu-Yu,Wei Qu-Fu
We developed a novel biocompatible material in the form of core-sheath nanofibers using coaxial electrospinning with tetracycline hydrochloride (TCH) as the model drug. To prepare nanofibrous membranes, poly(vinyl alcohol)-stilbazole quaternized (PVA-SbQ) and zein were first dissolved
in acetic acid as the sheath form while drug TCH as the core form. Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) images demonstrated that the nanofibers had a round and smooth surface morphology with an average diameter of 980±252 nm. It showed distinct
core-sheath structure at the sheath-to-core flow rate ratios of 1 and 0.5, respectively. Differential scanning calorimetry (DSC) and X-ray diffraction (XRD) results revealed that the tetracycline hydrochloride was well distributed in the PVA-SbQ/zein matrix in an amorphous state. Fourier transform
infrared spectroscopy (FTIR) analysis was used to verify the second-order interactions of the components, which revealed good compatibility of the components of the nanofibers. Thermal gravimetric (TG) analysis demonstrated that the core-sheath nanofibers improved the thermal stability of
TCH. The nanofibers showed good cytocompatibility toward L929 cells, further confirming their potential use as drug delivery and wound dressing. In vitro drug release studies indicated that the core-sheath nanofibers exerted sustained release instead of initial burst release effect,
and also followed the Fickian diffusion mechanism. The present study shows a simple but promising approach for the fabrication of novel biomaterials with structural characteristics for drug release profiles using coaxial electrospinning.
研究领域:
材料科学
·
药学
·
生物医学工程
✓ 已验证
DOI
高影响因子
Materials
·
2022
·Vol. 15
·期 8
·2795
·
IF 3.748
Dongwei Wei,Fanhui Zhou,Hongdi Wang,Guijin Liu,Jun Fang,Yanbin Jiang
In order to prepare edible films with outstanding antimicrobials and antioxidants utilized in applications of food and pharmaceutics, in this study, effects of surfactants on zein cast films for simultaneous delivery of lysozyme (LY) and ascorbic acid (AA) were investigated, where sodium alginate (SA), soy lecithin (SL), and Pluronic f-68 (PF-68) were selected as surfactants. FT-IR tests indicated that SL or PF-68 dramatically changed secondary structure of zein composite films, which heightened the irregularity of the composite film and inhibited LY crystallization. Mechanical tests showed that highly flexible films exhibiting elongations between 129% and 157% were obtained when adding PF-68. Compared with the film without emulsifier, zein film containing SL and PF-68 showed approximately 7.51 and 0.55 times lower initial release rates for LY and AA respectively, which significantly improved the controlled release and heightened the anti-microbial and anti-oxidant activities of the film. Finally, emulsified mechanisms of the surfactants in zein films were proposed.Keywords:zein;surfactant;lysozyme;ascorbic acid;controlled release;emulsified mechanism
研究领域:
食品科学
·
药剂学
·
材料科学
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）