DESALINATION
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2022
·
IF 11.211
Jiawei Liu,Zhen Wang,Wenwen Li,Xuliang Wang,Yanlei Su
A noticeable improvement of nanofiltration (NF) membranes with high separation performance for desalination was reported in this work. Graphene quantum dots (GQDs) enhanced polyamide (PA) composite membranes (GPA) were composed of porous substrates and nanofilms The nanofilms as separation layers were fabricated by the support-free interfacial polymerization and possessed ultrathin thickness below 8.0 nm. GQDs-PA interface channels and GQDs-GQDs interface channels gradually generated and penetrated the PA functional layer, resulting in a dramatic permeance increase of GPA membranes. The GPA membranes were creatively modified with arginine (Arg) to improve hydrophilicity and narrow the water channels. The Arg-GPA NF membranes under optimized preparation exhibited exceedingly high permeance of 40.3 L m −2 h −1 bar −1 with Na 2 SO 4 rejection rate of 99.7 %, and 60.7 L m −2 h −1 bar −1 with Na 2 SO 4 rejection rate of 99.4 %. This synergistic strategy of GQDs regulation on PA nanofilm structure and Arg post-treatment modification may provide a reliable and efficient technique for the design of NF membranes.
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Microbial Cell Factories
·
2022
·Vol. 21
·期 1
·1-12
·
IF 6.352
Yang Fengyu,Xu Jiayu,Zhu Yichun,Wang Yi,Xu Meijuan,Rao Zhiming
Background Agmatine is a member of biogenic amines and is an important medicine which is widely used to regulate body balance and neuroprotective effects. At present, the industrial production of agmatine mainly depends on the chemical method, but it is often accompanied by problems including cumbersome processes, harsh reaction conditions, toxic substances production and heavy environmental pollution. Therefore, to tackle the above issues, arginine decarboxylase was overexpressed heterologously and rationally designed in Corynebacterium crenatum to produce agmatine from glucose by one-step fermentation.Results In this study, we report the development in the Generally Regarded as Safe (GRAS) l-arginine-overproducing C. crenatum for high-titer agmatine biosynthesis through overexpressing arginine decarboxylase based on metabolic engineering. Then, arginine decarboxylase was mutated to release feedback inhibition and improve catalytic activity. Subsequently, the specific enzyme activity and half-inhibitory concentration of I534D mutant were increased 35.7% and 48.1%, respectively. The agmatine production of the whole-cell bioconversion with AGM3 was increased by 19.3% than the AGM2. Finally, 45.26 g/L agmatine with the yield of 0.31 g/g glucose was achieved by one-step fermentation of the engineered C. crenatum with overexpression of speAI534D.Conclusion sThe engineered C. crenatum strain AGM3 in this work was proved as an efficient microbial cell factory for the industrial fermentative production of agmatine. Based on the insights from this work, further producing other valuable biochemicals derived from l-arginine by Corynebacterium crenatum is feasible.
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RSC Advances
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2022
·Vol. 12
·期 5
·3157-3164
·
IF 4.036
Yi Xiao,Guofang Wang,Haomin Yi,Suhua Chen,Qinyu Wu,Siyi Zhang,Kexin Deng,Simeng Zhang,Zi-Qi Shi,Xiaoping Yang
Ru(bpy)3Cl2/TPrA is a prominent and widely used ECL system in analytical science. However, the co-reactant TPrA restricts the variety of applications because of its toxicity, volatility, and high cost. Here, we use arginine (Arg) as an alternative co-reactant for Ru(bpy)32+ by taking advantage of its low cost, non-toxicity, and biocompatibility. The mechanism of the Ru(bpy)32+/Arg system is that the deprotonated Arg can react with Ru(bpy)32+ to release emission. The similarity between the Ru(bpy)32+/Arg, Ru(bpy)32+/TPrA, and Ru(bpy)32+/DBAE systems demonstrates that Arg can be used as an alternative co-reactant for Ru(bpy)32+ ECL. As a proof of concept, we achieve an excellent performance for acetaminophen (Ace) detection based on the specificity of Arg and Ace, with excellent linearity, low detection limits, and good recoveries. This work is promising to expand the scope of the Ru(bpy)32+/Arg system and move forward their applications in bioassays.
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JOURNAL OF MEDICINAL CHEMISTRY
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2016
·
IF 5.589
Xiaoli Liu,Rui Cao,Sha Wang,Junli Jia,Hao Fei
Cationic amphipathic peptides (CAPs) are known to be able to cause membrane destabilization and induce cell death, yet how the hydrophobicity, amphipathicity, and lysine (K)/arginine (R) composition synergistically affect the peptide activity remains incompletely understood. Here, we designed a panel of peptides based on the well-known anticancer peptide KLA. Increasing hydrophobicity enhanced the cytotoxicities of both the K- and R-rich peptides. Peptides with an intact amphipathic helical interface can cause instant cell death through a membrane lysis mechanism. Interestingly, rearranging the residue positions to minimize amphipathicity caused a great decrease of cytotoxicity to the K-rich peptides but not to the R-rich peptides. The amphipathicity-minimized R-rich peptide 6 (RL2) (RLLRLLRLRRLLRL-NH2) penetrated the cell membrane and induced caspase-3-dependent apoptotic cell death. We found that the modulation of hydrophobicity, amphipathicity, and K/R residues leads to distinct mechanisms of action of cationic hydrophobic peptides. Amphipathicity-reduced, arginine-rich cationic hydrophobic peptides (CHPs) may represent a new class of peptide therapeutics.
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EUROPEAN POLYMER JOURNAL
·
2023
·
IF 6
Xin He,Jie Guan,Zhengpeng Chen,Zhengshuai Cao,Yunfan Li,Ziqiang Lei,Denglong Chen
In this study, bio-based L-arginine (Arg) and phosphite were selected as raw materials and used to modify magnesium hydroxide, generating an intermediate (APM) that was reacted with melamine (MEL) to produce a curing agent (APM-MEL) with flame retardant properties. A flame retardant epoxy resin (EP) was prepared in a curing reaction by adding APM-MEL and ammonium polyphosphate (APP) to the epoxy prepolymer in varying amounts (wt%). The chemical structure and surface morphology of APM-MEL have been characterized by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), and X-ray photoelectron spectroscopy (XPS). The thermal stability, flame retardancy and mechanical properties were evaluated by thermogravimetric analysis (TG), the UL-94 vertical combustion test, use of the limiting oxygen index (LOI), cone calorimetry and bending tests. The results have revealed that the epoxy resin prepared from the addition of 5% APM-MEL and 15% APP achieved a UL-94 V-0 level with a LOI of 30.8%. Compared with pure EP, the peak heat release rate, total heat release, smoke production rate and total smoke production of the samples were reduced by 85.68%, 53.15%, 85.86% and 62.05%, respectively. The flexural strength and flexural modulus were improved by 117.3% and 130.5%, respectively. The bio-based functionalized magnesium hydroxide curing agent prepared in this study exhibits excellent flame retardant and mechanical properties, effectively reducing fire risk and can serve as the basis for the development of multifunctional curing agents.
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ACS Sustainable Chemistry & Engineering
·
2021
·
IF 8.198
Zehai Chen,Hongzhan Chen,Youcai Che,Luo Cheng,Hao Zhang,Jian Chen,Fangyan Xie,Nan Wang,Yanshuo Jin,Hui Meng
Aqueous zinc-ion batteries are regarded as one of the new promising rechargeable batteries. However, serious Zn dendrite growth causes short-circuit issues in the batteries. Herein, we report that arginine (Arg), a cationic surfactant electrolyte additive, can inhibit irregular and nonplanar dendrite growth and induce uniform Zn deposition. It shows a long cycle life (515 h) of Zn/Zn cells in the electrolyte containing Arg, which is ∼10 times longer than that of the electrolyte with no additive. The hydrolysis of Arg produces arginine cations (Arg+), which will be preferentially adsorbed on Zn dendrites due to the tip charge accumulation effect during the Zn plating process. Arg+ increases the overpotential for the nucleation of Zn dendrites and results in uniform Zn deposition. After about 350 cycles, the MnO2/Zn cell shows 84.59% capacity retention and 99.85% average Coulomb efficiency in the electrolyte with the additive. The low-cost and nontoxic electrolyte additive identified in this work provides a new pathway toward inducing regular Zn deposition morphology.
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ANALYTICA CHIMICA ACTA
·
2020
·
IF 5.977
M.R. Ali,M.S. Bacchu,M. Daizy,C. Tarafder,M.S. Hossain,M.M. Rahman,M.Z.H. Khan
s In this experiment, a highly effective electrochemical sensor based on a molecularly imprinted polymer has been developed for ultrasensitive detection of dimetridazole. The sensor was made by incorporating of dimetridazole as a template molecule during the electropolymerization of poly-arginine on a glassy carbon electrode. The modified electrode GCE/P-Arg@MIP was characterized by voltammetric and microscopic techniques. Differential pulse voltammetry method was used to detect target analyte under the optimum condition. The DPV response to dimetridazole was linear at 0.1 × 10 −9 to 10 × 10 −6 mol L −1 ( R 2 = 0.996), with a method detection limit (S/N = 3) of 0.1 × 10 −9 mol L −1 . Moreover, the proposed sensor shows satisfactory recovery ranges for the determination dimetridazole in commercially available egg, milk and honey samples.
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SEPARATION AND PURIFICATION TECHNOLOGY
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2021
·
IF 7.312
Chunyu Zhang,Shoujing Qi,Jie Meng,Xuwei Chen
Arginine-functionalized amino flowered mesoporous silica (A-FMS-Arg) nanoparticles are fabricated via the amidation reaction, and taken as efficient sorbent for refined extraction of heparin. Attributed by the multiple H-bond interaction between the sulfate group of heparin and the guanidine group of Arginine, A-FMS-Arg nanoparticles exhibit favorable adsorption selectivity towards heparin. The three-dimensional flowered structure provides A-FMS-Arg with abundant adsorption sites, and the adsorption capacity is high to 173.71 mg/g. 1HNMR assay indicate that A-FMS-Arg nanoparticles can selectively adsorb heparin from the mixture of heparin/chondroitin, and the practicability of A-FMS-Arg is well demonstrated by the efficient extraction of heparin from digested porcine intestinal mucosa.
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Journal of Materials Chemistry B
·
2018
·Vol. 6
·期 24
·4116-4123
·
IF 4.776
Yue Zhang,Meng-Meng Wang,Jun-Xia Hao,Xu-Wei Chen,Jian-Hua Wang
The crosstalk between phosphoproteins and glycoproteins causes many difficulties in their selective isolation/enrichment from biological samples. This issue is of high significance in proteomics study, but thus far, it has not received proper attention. Herein, an arginine-functionalized polyhedral oligomeric silsesquioxane (POSS) framework, PP-x-Arg (x = 0, 1, 2, … denotes the amount of salt in preparation), was developed by combining salt-templated thermal polymerization of POSS and pyromellitic dianhydride (PMDA) with post-modification using arginine. PP-x-Arg possesses a porous nanostructure and abundant functional groups, namely, guanidine and zwitterionic groups, enabling the selective adsorption of phosphoproteins or glycoproteins via specific phosphate–guanidine affinity or hydrophilic interaction between PP-x-Arg and glycoproteins, respectively. In particular, the adsorption selectivity exhibited by PP-x-Arg can be easily regulated by adjusting the pH values of the adsorption medium. The PP-x-Arg framework was further employed for the discrimination and isolation of phosphoproteins and glycoproteins from biological samples.
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MACROMOLECULAR RESEARCH
·
2018
·Vol. 26
·期 13
·1241-1250
·
IF 1.767
Shen Xiang,Liu Peng,Xu Jiao,Liu Teng,Liu Jianjun,Shen Xianfu,Xia Shubiao,Wang Fan
Membrane technology has been accepted as a very effective approach to purify oily wastewater. However, the serious membrane fouling induced by the adsorption of oily foulants significantly hinders the development of membrane technology in treating oily wastewater. To improve the anti-fouling property and oil/water separation efficiency of membrane, this work reports on the covalent immobilization of short-chain arginine (Arg) molecules onto polyacrylonitrile/polyacrylonitrile- co -poly(glycidyl methacrylate) (PAN/PAN- co -PGMA) blend membrane surface via a ring-opening reaction between epoxy groups and amine groups. It was found that the covalent immobilization of Arg molecules effectively increased the surface hydrophilicity of the membrane, resulting in a significant decrease of the interaction force between the foulants and the membrane surface. This typical characteristic was revealed by the pure water contact angle and the force-extension curve measurements. The Arg-immobilized membranes exhibited much higher separation efficiency against oil/water emulsions than the pristine PAN and PAN/PAN- co -PGMA membranes. Especially when the grafting amount of Arg was 157.3 μg/cm 2 , the oil rejection ratio of an Arg-immobilized membrane was as high as 99.2%. In addition, the flux recovery ratio of the membrane still reached 88.8%, even after two cycle filtrations of pure water and oil/water emulsion. These results indicated that the Arg-immobilized membrane may have practical applications for oil/water emulsion separation.
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FOOD HYDROCOLLOIDS
·
2024
·
IF 11
Siqun Liu,Meirong Xu,Yuxin Guo,Yexia Xiao,Huifang Wang,Xianghua Gao,Xiaojie Lian,Baolong Niu,Wenfeng Li
Ag-based materials have potent antibacterial effects , but their potential toxicity problems should not be underestimated, so it is urgent to construct a safe and non-resistant dual antibacterial system. In this study, we utilized tea polyphenols (TPs) and montmorillonite (MMT) to control the growth of silver nanoparticles (AgNPs). Then, we combined the AgNPs with arginine to construct a synergistic antibacterial system of arginine-modified chitosan-assisted AgNPs-loaded MMT (ACS/G/AgNPs@MMT). The results showed that AgNPs with a uniform dispersion of 6.72 ± 4.66 nm and ACS with a grafting degree of 0.153 were successfully prepared, and the synergistic antibacterial effect between ACS and AgNPs@MMT was verified by the FIC index, and the antibacterial rate could still reach 99.9 % when the additions of ACS and AgNPs@MMT were reduced by 75 % and 79.1 %, respectively. The ACS/G/AgNPs@MMT-2 composite film exhibited excellent mechanical properties, water vapor/UV barrier properties, antioxidant activity , degradation properties, and biosafety. In addition, the synergistic effect enabled the ACS/G/AgNPs@MMT-2 film to achieve antibacterial rates of 99.24 ± 0.19 % against E. coli and 99.49 ± 0.40 % against S. aureus , and the film exhibited excellent long-lasting antibacterial performance due to the slow release of Ag + (only about 11.27 % of Ag + was released in 20 days). Finally, the ACS/G/AgNPs@MMT-2 film could significantly improve the food preservation ability. In conclusion, this study indicates that the film could be used as a novel antibacterial packaging material .
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JOURNAL OF MEMBRANE SCIENCE
·
2024
·
IF 8.4
Yichen Chen,Yan Yu,Liming Yang,Qingchun Ge
Purifying water contaminated with emulsified oil via forward osmosis (FO) is still plagued by low water recovery efficiency and poor fouling resistance of membrane. Herein we design a series of arginine-copper complexes to construct antifouling FO membranes from a polyamide membrane (PA-M) for oily water reclamation. A modification layer abundant in Cu 2+ and guanidino is formed on the PA selective surface. It endows the membranes with desired structural and physicochemical properties that benefit the FO separation. The membrane functionalized with copper arginine sulfate (CAS-M) outperforms PA-M with water fluxes enhanced by 151 % against DI water. CAS-M also achieves higher oil-water separation efficiency with the water recovery rate increased by 94 % compared to that of PA-M when 3000 ppm emulsified oil as the feed. Remarkably, CAS-M exhibits excellent antifouling and antibacterial performance with a 98 % antibacterial rate and easier cleaning property after being fouled. Favorable physicochemical and structural characteristics as well as advantageous water transfer properties are the major benefits of CAS-M. This makes CAS-M outperform PA-M and other recently reported FO membranes with exceptional water permeability and antifouling ability. This study demonstrates an eco-friendly protocol to develop antifouling and antibacterial FO membrane for sustainable oily water purification.
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