Science of Advanced Materials
·
2023
·Vol. 15
·期 12
·1621-1628
·
IF 0.9
Tang Hongmei,Deng Zhe,Zhao Tianlan
This research was aimed to explore the application of chitosan (CS) nanoparticles (NPs) loaded with vascular endothelial growth factor (VEGF) in rat skin injury. Method: the VEGF-loaded CS NPs (VEGF/CS-NPs) were prepared, and a skin injury model on the back skin of rats was established.
The VEGF/CS-NPs were applied to the injury model to observe the skin changes in the wound area of each group, compare the healing rate, the number of micro-vessels, and VEGF expression in each group of rats, so as to explore the intervention effect of VEGF/CS-NPs in skin injury. Results:
the prepared VEGF/CS-NPs were spherical or quasi-spherical in morphology, uniformly dispersed, with smooth surfaces and relatively uniform size. The average particle size (PS) of the blank CS-NPs was 267.5 nm± 25.3 nm, while that of the drug-loaded CS-NPs was 298.6 nm ±44.7 nm,
and the PS distribution was also very concentrated. The genipin cross-linked NPs could effectively prevent the degradation of the spherical wall and achieve the slow release of VEGF. After VEGF was applied to rats in the form of NPs, its healing rate and micro-vessel count were markedly superior
to those of VEGF injection alone and blank group (P < 0.05), and VEGF expression in skin injury tissues was also greatly increased (P < 0.05). Conclusion: VEGF/CS-NPs could improve the bioavailability of VEGF and promote the healing of rat skin injuries.
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CARBOHYDRATE POLYMERS
·
2026
·
IF 13.2
De-yang Huo,Kai Zheng,Zhao-feng Tian,Ling-jie Song,Li-chun Zhao,Wei Li
Burn wound healing is hindered by persistent inflammation, bacterial infection, and delayed tissue regeneration. Here, we developed DCNPs-Gel, an injectable, self-healing hydrogel incorporating dihydroquercetin (DHQ)-loaded chitosan nanoparticles (DCNPs; ~320 nm, PDI = 0.21, encapsulation efficiency 87.6%, drug loading 7.3%) into a dual-dynamic crosslinked oxidized sodium alginate (DO ~38%)/carboxymethyl chitosan network via Schiff-base and boronate ester bonds. DCNPs-Gel exhibited rapid self-healing (~96% modulus recovery within seconds), shear-thinning injectability (viscosity decreased ~3 orders of magnitude from 0.1 to 100 s −1 ), storage modulus ~830 Pa, high swelling ratio (1803%), and sustained DHQ release (~65% at 72 h). Bacteriostasis rates exceeded 90% against both E. coli (90.93%) and S. aureus (91.33%). Hemostatic evaluation demonstrated DCNPs-Gel reduced blood loss to ~200 mg versus ~580 mg (control) in the liver perforation model. In vitro , DCNPs-Gel maintained >90% HUVEC viability, reduced intracellular ROS by ~72%, and promoted scratch closure. In vivo , DCNPs-Gel accelerated deep second-degree scald wound closure to ~95.2% by day 18, enhanced collagen deposition and neovascularization. Mechanistically, DCNPs-Gel downregulated TLR4/NF-κB/IL-6/COX-2, upregulated VEGFA, rebalanced IL-6/IL-10 cytokine levels, and restored skin microbiota homeostasis. DCNPs-Gel uniquely integrates nanoparticle-mediated sustained release with dual-dynamic crosslinking, synergistically addressing oxidative stress, infection, hemostasis, and microbiota dysbiosis for comprehensive burn wound management.
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CARBOHYDRATE POLYMERS
·
2021
·
IF 9.381
Shaoxiang Lee,Meng Zhang,Guohui Wang,Wenqiao Meng,Xin Zhang,Dong Wang,Yue Zhou,Zhonghua Wang
In this paper, polyvinyl alcohol/starch composite films with p-coumaric acid modified chitosan (P-CS) and chitosan nanoparticles (P-CSNPs) at different concentrations were successfully prepared. The films were compared for their mechanical, thermal, physical, antioxidant, antibacterial, cytotoxicity and optical barrier properties. The results suggested that P-CS could significantly increase the tensile strength (TS) of the film from 15.67 MPa to a maximum of 24.32 MPa. The compact structure of P-CSNPs film prevented water diffusion, reducing the water amount within. Both films showed a reduction in water solubility, the extent of swelling, and water vapor transmittance. Specifically, P-CSNPs films showed better thermal stability while P-CS films revealed higher antioxidant activity. Besides, the P-CS films exhibited excellent transparency and good ultraviolet-barrier at 200−280 nm. P-CSNPs films demonstrated higher antibacterial activity on both Gram-negative and Gram-positive bacteria. Additionally, P-CS films were less cytotoxic compared to P-CSNPs films.
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COLLOIDS AND SURFACES B-BIOINTERFACES
·
2017
·
IF 3.887
Jing Cao,Yahui Zhang,Yukun Wu,Jing Wu,Wei Wang,Qiang Wu,Zhi Yuan
It has been shown that multivalent ligands could significantly enhance the binding avidity compared with the monovalent ones; therefore, once incorporated into nanoparticles, they promote superior targeting ability without increasing the ligand density. Although ligand valency and density play a key role on the targeting ability of corresponding nanoparticles, these facotrs remain largely unexplored and detailed studies are lacking. Herein, a series of multivalent ligands with certain valencies (FA n , n indicates the valency of ligand: n = 3, 5, 7) has been conveniently synthesized by conjugating different copies of folate ligands with poly(acrylic acid) (PAA). Negatively charged chitosan nanoparticles (CTS-SA NPs) have been utilized as proper multivalent platforms because they can strongly suppress non-specific protein adsorption and cellular uptake without interfering with the targeting ability of multivalent ligands. Subsequently, the structure of CTS-SA NPs has been modified using different amounts of FA n to form multivalent nanoparticles (FA n -CTS-SA NPs) with various valencies and densities. A series of specific investigations of them suggested that the cellular uptake of multivalent nanoparticles has largely varied with the ligand valency variation even at similar ligand densities; and also largely varied with ligand density variation even at the same ligand valencies. The intermediate valency and density values determined in the current study ( ie., 5 and 2.4 wt%, respectively) have provided the best cellular uptake, facilitating superior targeting ability at relatively low ligand valency and density. Unexpectedly, no conspicuous difference has been observed during endocytotic inhibition assays with single inhibitors, which may be attributed to the synergetic endocytotic mechanism with multiple pathways of multivalent nanoparticles. The optimal multivalent nanoparticles have also exhibited excellent biocompatibility, long-term stability in vitro and enhanced circulation time in vivo , thus demonstrating their potential for targeted drug delivery.
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Materials Science & Engineering C-Materials for Biological Applications
·
2017
·
IF 4.164
Chongyang Wang,Wenxiu Hou,Xuran Guo,Juehong Li,Tu Hu,Manle Qiu,Shen Liu,Xiumei Mo,Xudong Liu
Growth factor is an essential ingredient to regulate mesenchymal stem cells (MSCs) chondrogenic differentiation in cartilage tissue engineering. However, non-osteochondral specification, short plasma half time and bioactivity loss restrict growth factor's application. Thus, novel chondrogenic growth factors, specifically target osteochondral lineage cells, that can be sustained release and bioactivity protected to exert functions continually and effectively have attracted increasing researchers' interest. To achieve these goals, chitosan nanoparticles and electrospun fiber scaffolds were used as dual release system to sustain release Nel-like molecule-1 (Nell-1) growth factor and protect bioactivity, then the effect and mechanism of Nell-1 on inducing human bone MSCs (hBMSCs) differentiate toward chondrocytes were investigated. For release and bioactivity protection study, preloading Nell-1 into chitosan nanoparticles significantly extended the release time, increased the released Nell-1's bioactivity than directly incorporating Nell-1 into the scaffolds. Furthermore, Nell-1 specifically promotes hBMSCs in vitro chondrogenic differentiation by increasing expression of chondrogenic related genes and proteins. These findings suggest the potential utility of Nell-1 incorporated dual release scaffold for cartilage tissue engineering.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Kehui Wang,Xiangjun Yang,Junjun Liang,Yan Rong,Weijie Zhao,Jiahao Ding,Yiming Liu,Qi Liu
This study employed a precipitation method to synthesize zinc oxide@quaternised chitosan nanoparticles (ZnO@QAC NPs) containing different concentrations of zinc oxide, namely ZnO@QAC-2, ZnO@QAC-4, and ZnO@QAC-6. Subsequently, these nanoparticles were incorporated into matrices consisting of gelatine (Gn) and polyvinyl alcohol (PVA) separately, which were prepared by casting to form a biodegradable film. We assessed the physicochemical properties of ZnO@QAC NPs and physicochemical characteristics, antioxidant properties, antimicrobial activity and grape preservation efficacy of the film. Compared to the control group, the films showed a reduction in water vapor permeability by >9.38 %, an increase in tensile strength by over 51.95 %, over 70 % scavenging of ABTS free radicals, and good biocompatibility. Additionally, the antimicrobial activity of the films containing ZnO@QAC-6 increased by 37.6 %. In the grape preservation experiment, the weight loss of grapes wrapped in ZnO@QAC-2 film was reduced by 40.13 % on day 15 compared to unwrapped grapes. These results demonstrate that ZnO@QAC/PVA/Gn films have considerable potential for food packaging applications.
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Food Packaging and Shelf Life
·
2024
·
IF 8.5
Xu Fan,Lingyu Yin,Jiamin Zhu,Pengyuan Sun,Yingao Zhu,Qian Chen,Baohua Kong,Qian Liu,Hui Wang
The effect of coating of oregano essential oil loaded zein pectin chitosan nanoparticles (Zein-PC-CS-OEO) to improve the quality of Harbin red sausage during storage was investigated. Firstly, Zein-PC-CS-OEO nanoparticles were prepared and characterized. Subsequently, Zein-PC-CS-OEO nanoparticles (EN-OEO) added sodium alginate solutions were successfully coated on the Harbin red sausage at different concentrations. All the sausages were stored at 20 °C and 65% relative humidity for 12 d, and subsequently sampled at 0, 3, 6, 9, and 12 d. The physicochemical properties, total number of aerobic bacteria, bacterial community structure and flavor characteristics for the Harbin red sausage were measured. Both lipid oxidation and the growth of dominant spoilage bacteria in Harbin red sausage are inhibited by EN-OEO coating, while the total aerobic bacteria count is reduced. These results indicate that the storage quality of Harbin red sausage is improved by EN-OEO coating. It is worth noting that the shelf life of the Harbin red sausage with 3% EN-OEO coating is extended to 9 d and the flavor characteristics of Harbin red sausage can be better preserved compared with the Control sample and the unencapsulated samples. This study provides a new thought for the preservation of Harbin red sausage as well as other low-temperature meat products.
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Journal of Biomedical Nanotechnology
·
2017
·Vol. 13
·期 3
·243-254
·
IF 4.521
Jiang Dandan,Zhang Xiaopeng,Yu Dexin,Xiao Yanan,Wang Tianqi,Su Zhihui,Liu Yongjun,Zhang Na
Magnetic resonance imaging (MRI) contrast agents with tumor-microenvironment changeable relaxivity are effective to increase the sensitivity and selectivity of MRI in tumor diagnosis. In this study, pH-sensitive Gd-loaded Poly(L-lysine)/ Carboxymethyl Chitosan Nanoparticles (Gd-PCNPs)
were developed as relaxivity-changeable MRI contrast agents based on the "on–off" switchable strategy. The "on–off" switchable nano-contrast agents were capable of releasing Gd3+ in response to physical stimulation, with structure transformed. Gd-PCNPs could responsively
disassemble in an acidic tumor-microenvironment and increase the exchange of protons between water molecules and Gd3+ ions, thus selectively enhance the relaxivity in tumor area. Gd-PCNPs were self-assembled via electrostatic interaction between poly(L-lysine)-diethylenetriamine
pentaacetic acid-gadolinium and pH-sensitive carboxymethyl chitosan (CMCS). Gd-PCNPs exhibited spherical shape with uniform particle size distribution (166.00 ± 1 .71 nm) and negative zeta potential (–13.2 ± 4.7 mV). The relaxivity of Gd-PCNPs increased from 6.618 mM–1
· s–1 to 10.008 mM–1 · s–1 when the pH values decrease from 7.4 to 6.0, which was higher than Magnevist® (3.924 mM–1 · s–1 at both pH 7.4 and 6.0 (p <0 05). The
changeable relaxivity of Gd/PCNPs would result in enhanced tumor/normal tissue signal contrast, which was verified by in vivo MRI test. In vivo MRI test showed that the signal of Gd-PCNPs was significantly enhanced with prolonged imaging time in tumor tissue compared to Magnevist®
(p <0 05). Furthermore, Gd-PCNPs exhibited unobvious in vitro cytotoxicity under the experimental concentrations in B16 cells. No obvious damage was observed in the different tissues of mice. These results indicated that the relaxivity-changeable Gd-PCNPs exhibited demonstrated
sensitivity and selectivity in tumor diagnosis with a great potential as a novel MRI contrast agent.
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Polymers
·
2022
·Vol. 14
·期 19
·3972
·
IF 4.967
Fawzia A. Alshubaily,Ebtihaj J. Jambi
Various therapies for osteoporosis successfully reduce bone loss and fractures, but they mostly do not contribute to new bone structures and adversely affect patients. Shilajit is a natural mineral substance comprised of multi-components, with proved efficacy to improve immunity, antioxidant activity, and disease resistance. In the present study, various effects of shilajit water extract (SWE) on bone development and its management were determined in experimental glucocorticoid-induced osteoporotic rats. The fabrication of nanochitosan (NCT) and NCT conjugation with SWE were conducted and evaluated as enhanced formulations for treating osteoporosis. NCT and SWE/NCT had mean particle diameters of 196.4 and 248.4 nm, respectively, with high positivity charging and stability. The biochemical and anti-osteoporotic effects of SWE and SWE/NCT conjugates were investigated on different groups of compromised rats. Five groups each including six adult albino female rats were formed and treated for a duration of eight weeks with SWE and SWE/NCT conjugate. Significantly improved serum calcium, phosphorus, osteocalcin, and calcitonin levels but decreased hydrogen peroxide, IL-6, and antioxidant biomarkers were recorded in all SWE- and SWE/NCT-treated groups; the SWE/NCT treatment was most effectual treatment. These results suggest that SWE and SWE/NCT may cause anti-osteoporotic activity by reducing oxidative stress, IL-6, and H2O2while restoring antioxidant levels. The conjugation of SWE onto NCT is highly recommended for augmenting their activities.Keywords:nanopolymers carriers;nano-conjugation;shilajit extract;in vivo;antioxidant biomarkers
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FOOD CONTROL
·
2021
·
IF 5.548
Ran Zhao,Weiliang Guan,Pingan Zheng,Fang Tian,Zhenzhe Zhang,Zhidong Sun,Luyun Cai
This research was conducted to assess the structure, mechanical, barrier and optical properties of three edible composite films based on chitosan nanoparticles. Films containing cinnamon-perilla essential oil (C-PEO) Pickering nanoemulsion had denser structure and better mechanical properties. In addition, films with anthocyanidins exhibited enhanced antioxidant activity . The results showed that both Pickering nanoemulsion and anthocyanidins could improve some properties of edible composite film based on chitosan nanoparticles. Finally, preservation experiments were carried out. Analysis of storage quality indices (sensory evaluation, microbiological analyses, pH, total volatile basic nitrogen, thiobarbituric acid values, color and texture) revealed the shelf life of fillets wrapped in three kinds of composite film was extended to 6–8 days. The preservation effect of plant essential oil compound anthocyanidin edible film for fillet was the best and anthocyanidins compound chitosan nanoparticles edible film was the best in appearance of protection.
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JOURNAL OF CONTROLLED RELEASE
·
2015
·
IF 7.705
Min Liu,Jian Zhang,Xi Zhu,Wei Shan,Lian Li,Jiaju Zhong,Zhirong Zhang,Yuan Huang
Oral administration of protein drugs is greatly impeded by the lack of drug carriers that can efficiently overcome the absorption barriers of mucosa tissue, which consists of not only epithelium but also a blanket of mucus gel. We herein report a novel self-assembled nanoparticle (NP) platform for oral delivery of insulin by facilitating the efficient permeation through both of these two barriers. The NP possesses a core composed of insulin and trimethyl chitosan (TMC), and a dissociable “mucus-inert” hydrophilic coating of N-(2-hydroxypropyl) methacrylamide copolymer (pHPMA) derivative. The NPs exhibited free Brownian motion and excellent permeability in mucus, which enabled the access of the NP core to the epithelial cell surface underneath the mucus. Moreover, investigation of NP behavior showed that the pHPMA molecules started to dissociate as the NP permeates through mucus, and the TMC NP core was then exposed to facilitate transepithelial transport via paracellular pathway. The pHPMA coating significantly improved transepithelial transport of TMC-based NP and their ability to open tight junctions between the mucus-secreting epithelial cells. Moreover, in diabetic rats, pHPMA coated NPs generated a prominent hypoglycemic response following oral administration, and exhibited a relative bioavailability 2.8-fold higher than that of uncoated TMC-based NPs. Our study provided the evidence of using pHPMA as “mucus-inert” agent to enhance mucus permeation of TMC-based NPs, and validated a novel strategy to overcome the multiple absorption barriers using NP platform with dissociable hydrophilic coating and TMC-based core possessing tight junction-opening ability.
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JOURNAL OF BIOMATERIALS APPLICATIONS
·
2020
·
IF 2.22
Ke Ma,Yongbin Cheng,Xinran Wei,Daijun Chen,Xiaoli Zhao,Pengxiang Jia
In this work, gold embedded chitosan nanoparticles (Au@CS NPs) were fabricated by a one-pot method. The benzaldehyde-terminated poly[(2-methacryloyloxy) ethyl phosphorylcholine] (PMPC) was applied to modification of the gold doped chitosan nanoparticles. The obtained Au@CS-PMPC NPs had the diameter of 135 nm with a narrow distribution. The size of the Au@CS-PMPC NPs, as well as the size of the embedded gold NPs, might be well-controlled by adjusting the feeding ratio between chitosan and HAuCl4. Furthermore, the Au@CS-PMPC NPs showed increased colloidal stability, high drug loading content, pH-responsive drug release, excellent biocompatibility and bright fluorescence emission. The results demonstrated that Au@CS-PMPC NPs showed a great potential for tumor therapy via the combination advantages of pH-sensitive controlled drug release and cellular fluorescence imaging.
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