SEPARATION AND PURIFICATION TECHNOLOGY
·
2025
·
IF 9
Yinghong Wang,Ping Xiong,Wei Yang,Zhitong Zhang,Xiaoling Xu,Liming Yang,Huiji Xiao,Kai Zhang,Zhenzhou Wang,Wei Zhang,Xubiao Luo
Highly selective Mg 2+ /Li + separation membranes hold significant application prospects in extraction of lithium. However, the high Mg 2+ /Li + concentration ratio commonly found in brines and the very close hydrated ionic radii of the two ions limit the separation efficiency of conventional membranes. This study proposes a dual-phase supramolecular synergistic sieving nanofiltration membrane design strategy. Leveraging the unique molecular recognition unit and hydrophilic external interface characteristics of γ -cyclodextrin ( γ -CD), combined with the multifunctional positively charged groups of histidine, the strategy achieves the directional construction of highly efficient Mg 2+ /Li + sieving channels within the membrane. The strategy is accomplished through the synergistic action of γ -CD’s selective capture of high-valent Mg 2 + and histidine’s electrostatic repulsion effect. During the Mg 2 + /Li + separation process, the fabricated γ -CD-histidine composite membrane (HCF membrane) demonstrates outstanding comprehensive performance. It achieves a remarkably high Li + /Mg 2+ separation factor of 28.98 and maintains excellent separation performance across a wide Mg 2+ /Li + ratio range (1:1–100:1). The enhanced hydrophilicity of the HCF membrane significantly boosts permeate flux, while also exhibiting long-term operational stability and biofouling resistance during continuous operation under high Mg 2+ /Li + ratios. This research enables the high-selectivity separation of Mg 2+ and Li + with concurrent Li + enrichment, demonstrating significant potential for lithium extraction from salt-lake.
研究领域:
膜科学
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超分子化学
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分离技术
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材料科学
·
电化学
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产品关联
DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2024
·
IF 6.7
Lingyu Nie,Hui Zhang,Weiheng Kong,Rong-Mei Kong,En-Sheng Zhang,Jin Li,Yan Zhao,Fengli Qu
Designing artificial mimetic enzymes with high activity/selectivity to replace chiral bioenzymes is of great interest in the development of chiral materials consisting of molecules, enantiomers, that exist in two forms as mirror images of one another but cannot be superimposed. In this study, the chiral catalytic structural unit was streamlined from tyrosinase to integrate a mimetic nanozyme. The chiral amino acid l-histidine, as the chiral binding/recognition site, and the active metal site Cu were coupled (Cu@l-His) to create a copper–histidine brace with enantioselective catalytic ability to tyrosinol enantiomers. Results of kinetic parameters and activation energies confirmed the excellent peroxidase-like activity with a preference of Cu@l-His to l-tyrosinol. Such a preference could be attributed to the structurally oriented copper–histidine brace with a stronger affinity and catalytic activity to l-tyrosinol. By accurately evaluating chiral recognition units derived from bioenzymes, stable and superior chiral mimetic nanoenzymes could be constructed in a more straightforward and simplified manner, and they could also be extended to the reconstruction of diverse chiral enzymes.
研究领域:
生物化学
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纳米技术
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催化
·
手性材料
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产品关联
DOI
PubMed
高影响因子
ACS Applied Polymer Materials
·
2024
·
IF 4.4
Yuqing Shi,Jie Liu,Jianwei Xin,Bo Han,Yuqi Zhang,Ji-Jiang Wang
Histidine (His) is an essential amino acid that is closely related to the physiological activities of the human body and human health, being used as a biomarker and therapeutic drug for some diseases. It is desirable to accurately and facilely detect His. Here, an l-histidine (l-His)-sensitive smart two-dimensional photonic hydrogel sensor (CD-2DPCH) was fabricated by modifying NH2-β-cyclodextrin (NH2-β-CD) onto the poly(acrylamide-Co-acrylic acid) hydrogel containing polystyrene two-dimensional photonic crystal array (PS-2DPC). The volume of the CD-2DPCH sensor shrinks upon response to l-His due to the host–guest interaction between NH2-β-CD attached in the hydrogel chains and l-His and the hydrogen bonding interaction between l-His and amides groups on the hydrogel chains. The particle spacing change of ∼95 nm for the PS-2DPC in the hydrogel is achieved, and the chiral recognition of l-His is realized. The particle spacing change of the prepared CD-2DPCH shows a good linear correlation when the l-His concentration changes from 0.1 to 1 and 10 to 400 μM, and the limit of detection is 58 nM. The constructed smart photonic hydrogel sensor was successfully applied to determine l-His in human serum and milk. The fabrication of the sensor is facile, and the detection does not need sophisticated instruments, providing a facile and effective way to identify l-His and a good strategy for sensing other amino acids by modifying different recognition groups.
研究领域:
生物化学
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传感技术
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材料科学
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产品关联
DOI
高影响因子
CyTA-Journal of Food
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2019
·
IF 1.605
Tong Shi,Li Yuan,Jianlou Mu,Ruichang Gao
Amino acids containing L-Arginine (Arg), L-Lysine (Lys) and L-Histidine (His) are recognized to increase the solubility of myosin at low ionic strength, but there is a difference in the researchers’ views on their effects on the secondary structure of myosin. To elucidate which method is relatively appropriate for the determination of secondary structures of myosin added with Arg, Lys or His, Circular dichroism (CD) spectra and Raman spectroscopy were carried out respectively. Raman spectroscopy was more suitable than CD method for determination of the secondary structures of myosin added with His, while CD spectra was more accurate for the myosin samples added with Lys and the adequate concentration of Lys should be under 10 mM. Both CD and Raman methods could be applied to myosin added with Arg, however, the latter was recommended and the adequate additive content of Arg in CD method should be less than 10 mM.
研究领域:
生物化学
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分子生物学
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蛋白质研究
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DOI
高影响因子
Materials Today Bio
·
2021
·
IF 7.348
H. Xu,B. Tang,W. Huang,S. Luo,T. Zhang,J. Yuan,Q. Zheng,X. Zan
Because of their high specificity and low side effects, protein drugs possess a substantial global market. However, the low bioavailability of protein is still a major obstacle to their expanded applications, which is expected to be answered with proper protein formulations. Taking corneal neovascularization (CNV) as an example, we demonstrated a co-assembled system of hexa-histidine and Ava (Avastin) with metal ions ( [email protected] ) could cross the cornea, the most important bio-barrier during the treatment of most diseases of the anterior segment in clinics. We found that the nanosized [email protected] efficiently encapsulated Ava with impressive loading capacity without destroying the bioactivity of Ava and assisted Ava penetration through the corneal barriers to effectively inhibit CNV development in an alkali burn rat model with sustained and pH-dependent Ava release. Our results suggested that the co-assembled strategy of protein and HmA is a proper formulation to protein drugs, with promising penetration ability to deliver protein across bio-barriers, which could open a path for topical administration of protein drugs for treatment of various ocular diseases and hold enormous potential for delivery of therapeutic proteins not only for ocular diseases but also for other diseases that require protein treatment.
研究领域:
药剂学
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眼科
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生物医学工程
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DOI
PubMed
高影响因子
BIOMACROMOLECULES
·
2018
·
IF 5.738
Yuchen Guo,Sha Wang,Hanhan Du,Xiaolong Chen,Hao Fei
Understanding the chemical absorption process of silver ions helps the rational design of functional materials for effective release to minimize unwanted toxicity. To this end, a histidine-containing aliphatic peptide (IH6) was designed to immobilize the silver ion (Ag+) through coordinate interaction. Using circular dichroism spectroscopy, Ag+ was found to dose-dependently induce parallel β-sheet conformation of IH6 to a saturation molar ratio of 1:2. A conformational switch of IH6 from antiparallel to parallel β-sheet assembly upon Ag + coordination was further revealed by Fourier transform infrared spectroscopy. The resultant Ag-IH6 hydrogel displayed substantially enhanced mechanical strength as well as controlled release of Ag+. Ag-IH6 hydrogel thus exhibited strong dose-dependent bactericidal activities that can be tuned selectively, sparing the cocultured human keratinocytes in normal. Overall, the study demonstrates an unusual silver ion-induced peptide conformational switch between β-structure subtypes and the bilateral effects on hydrogel-based chemical control of silver ion absorption and release, thus, revealing the potential in antibacterial applications.
研究领域:
生物化学
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材料科学
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微生物学
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DOI
PubMed
高影响因子
FOOD CHEMISTRY
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2019
·
IF 5.399
Ruichang Gao,Tong Shi,Quancai Sun,Xiuting Li,David Julian McClements,Li Yuan
This research focused on the effects of l -arginine ( l -Arg) and l -histidine ( l -His) on the heat-induced aggregation of fish myosin. l -Arg/ l -His increased the pH of the myosin solution from 6.82 to 8.74 and 7.24, respectively, and decreased the turbidity, aggregate size, shear modulus, and breaking force. The incorporation of l -Arg/ l -His decreased the surface hydrophobicity during setting, but increased it during the two-step heating. The heat-induced aggregation of myosin was suppressed by both amino acids, with the inhibitory effect being greater for l -Arg than l -His. On one hand, the change in the pH played a critical role in suppressing the heat-induced aggregation of myosin. On the other hand, the characteristics of l -Arg/ l -His themselves, such as net charges and particular R-groups, were another main contributor to aggregation suppression. Particularly, l -Arg/ l -His could interact with exposed aromatic residues of myosin, and the interactions may dominate and overwhelm the burial of aromatic residues during two-step heating.
研究领域:
食品科学
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生物化学
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产品关联
DOI
PubMed
高影响因子
Nanoscale
·
2019
·Vol. 11
·期 37
·17401-17406
·
IF 6.97
Yue Hua,Yuanyuan Cai,Huan Liu,Yuqi Wan,Xiju Ding,Shuai Li,Hua Wang
A highly selective and sensitive electroanalysis strategy has been developed for sensing histamine (HTA) and histidine (His) with “turn-on” signal outputs using copper nanocomposites (Cu NCs) of reduced copper metal–organic frameworks (Cu MOFs). It was discovered that the Cu NC-modified electrodes could display the sharp and stable oxidation peaks of solid-state CuCl electrochemistry at a low potential (about −0.10 V). More interestingly, once HTA or His was introduced, the peaking currents of the electrodes would increase due to the specific interaction between Cu2+ and imidazole groups of HTA or His. A highly selective electroanalysis method was thereby developed for the detection of both HTA and His in the concentration range of 0.010–100 μM. Besides, the application feasibility of the developed electroanalysis strategy was demonstrated for the evaluation of HTA and His separately in red wine and urine samples. Such an electroanalysis candidate for HTA and His holds great potential for wide applications in the fields of food analysis and clinical disease diagnosis.
研究领域:
分析化学
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生物医学工程
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食品科学
·
临床诊断
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ELECTROPHORESIS
·
2012
·Vol. 33
·期 5
·856-865
·
IF 3.303
Bo Pang,Jing Shao,Jie Zhang,Jia-Zhen Geng,Liu-Yin Fan,Cheng-Xi Cao,Jing-Li Hou
In this paper, a novel mode of free-flow affinity electrophoresis (FFAE) was developed to indirectly enhance the separation of free-flow electrophoresis (FFE). In the mode of FFAE, a Ni(II) with high electric charge density and histidine (His) is chosen as a model ligand and target solute, respectively. Through the controlling of experimental conditions (10 mM pH 6.0 Na 2 HPO 4 –NaH 2 PO 4 with 2.0 mM NiCl 2 ·6H 2 O background buffer), Ni(II) can combine with His and the combination leads to the high electric charge density of affinity complex of His-Ni(II) in contrast to the low density of free His molecule. But the ligand has weak interaction with uninterested amino acids. Thus, the mobility of His existing as His-Ni(II) is greatly increased from 14.5×10 −8 m 2 V −1 s −1 to 30.2 × 10 −8 m 2 V −1 s −1 , while those mobilities of uninterested amino acids are almost constant. By virtue of the mode, we developed the FFAE procedure and conducted the relevant experiments. The experiments demonstrated the following merits of the FFAE technique: (i) clear enhancement of separation between the target solute of His and uninterested amino acids; (ii) simplicity, and (iii) low cost. Furthermore, the technique was used for the continuous separation of His from its complex sample, and the purity of His was near to 100%. All of the results demonstrate the feasibility of affinity separation in FFE. The developed FFAE may be used in the separation and pretreatment of some biological molecules (e.g. peptides).
研究领域:
生物化学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF CHEMICAL AND ENGINEERING DATA
·
2023
·
IF 2.6
Xia Li,Weiping Liu,Yahui Yin,Yu Zhou,Hongfei Xiang,Xianzhen Xu
Vapor–liquid equilibrium (VLE) is essential for separation processes, such as distillation and absorption in chemical production. In this work, the VLE of glycine, l-histidine, and l-tryptophan aqueous solutions was measured at pressures ranging from 5.3 to 101.1 kPa. Xu’s model was used, and the model was based on the nonrandom two-liquid (NRTL) model, which has been successfully correlated with VLE data at temperatures between 298.15 and 373.85 K, and the model parameters were obtained. In addition, the absorption peaks of three amino acid aqueous solutions were analyzed by Fourier transform infrared (FTIR) spectroscopy from a microscopic perspective. The results showed that the higher the solubility of amino acids in water, the stronger the hydrogen-bonding interaction with water. Furthermore, the total mean interaction energy of water in the systems was studied by using a COSMOthermX 2021 tool. As inferred from the calculations, the total mean interaction energy was slightly affected by the concentration. The structure–activity relationship was established between the microscopic interactions and the macroscopic VLE.
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产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2023
·
IF 8.2
Wenmin Wu,Qixing Jiang,Pei Gao,Dawei Yu,Peipei Yu,Wenshui Xia
The effects of the L-hisdine (L-His)-assisted ultrasound on physicochemical characteristics and conformation of myofibrillar protein (MP) under reduced-salt condition were investigated using spectroscopic analysis , and the binding mechanism between L-His and MP was further elucidated through molecular docking and molecular dynamics (MD) simulations. UV second derivative spectra and intrinsic Try fluorescence spectra revealed that L-His formed a complex with MP and altered the microenvironment of MP. After L-His-assisted ultrasound treatment, MP showed smaller particle size, higher solubility, and more uniform atomic force microscopy image due to the decrease of α -helix content and the subsequent increase in zeta potential , active sulfhydryl content, and surface hydrophobicity . Molecular docking and MD simulations demonstrated the optimal docking pose (minimum binding affinity of −6.78 kcal/mol) and revealed hydrophobic interactions and hydrogen bonds as the main interaction forces between L-His and MP, with several residues (ILE-464, ILE-480, THR-483, ASN-484, GLY-466, ASP-463, PHE-246) identified as binding sites.
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产品关联
DOI
PubMed
高影响因子
Nanoscale
·
2015
·Vol. 8
·期 4
·2205-2211
·
IF 7.394
Xiaohua Zhu,Tingbi Zhao,Zhou Nie,Zhuang Miao,Yang Liu,Shouzhuo Yao
In this work, nitrogen-doped carbon nanoparticle (N-CNP) modulated turn-on fluorescent probes were developed for rapid and selective detection of histidine. The as synthesized N-CNPs exhibited high fluorescence quantum yield and excellent biocompatibility. The fluorescence of N-CNPs can be quenched selectively by Cu(II) ions with high efficiency, and restored by the addition of histidine owing to the competitive binding of Cu(II) ions and histidine that removes Cu(II) ions from the surface of the N-CNPs. Under the optimal conditions, a linear relationship between the increased fluorescence intensity of N-CNP/Cu(II) ion conjugates and the concentration of histidine was established in the range from 0.5 to 60 μM. The detection limit was as low as 150 nM (signal-to-noise ratio of 3). In addition, the as-prepared N-CNP/Cu(II) ion nanoprobes showed excellent biocompatibility and were applied for a histidine imaging assay in living cells, which presented great potential in the bio-labeling assay and clinical diagnostic applications.
研究领域:
分析化学
·
生物医学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）