MEASUREMENT
·
2023
·
IF 5.6
Wenjing Wang,Yumin He,Lei Deng,Hui Wang,Xiaoying Liu,Qing-wen Gui,Zhong Cao,Zemeng Feng,Benhai Xiong,Yulong Yin
L-Lysine (L-Lys) quantification in serum using a novel and highly selective amperometric biosensor has been reported. In this study, an efficient, enzyme-free, and simple anti-fouling biosensor was developed based on a self-screened peptide aptamer. A glassy carbon electrode (GCE) was used to construct anti-fouling interfaces by modifying its surface with polyaniline (PANI) polymer. The peptide aptamer, Cys-Pro-Pro-Pro-Pro-Arg-Glu-Asn-Ile-Gln-Arg-Leu-Thr, was then immobilized onto this electrode via an electroactive cysteine linker and its potential in the determination of L-Lys was examined. Under optimised experimental conditions, the peptide-modified electrodes exhibited excellent anti-fouling and electrochemical sensing. The biosensor was effective in resisting biofouling in a wide range of serum samples and amino acid solutions, and its linear range for L-Lys detection ranged from 1 nM to 10 mM, with a comparatively lower detection limit (0.3 nM; S/N = 3). The anti-fouling biosensor could detect L-Lys in real serum samples, and this approach of designing peptide aptamers based low-fouling biosensors can easily be extended to the development of a bio-sensing platform system for a variety of other metabolites.
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LWT-FOOD SCIENCE AND TECHNOLOGY
·
2023
·
IF 6
Yanan Zhao,Xinyu Zu,Ting Wang,Yan Liang,Yingqiu Li,Chenying Wang,Xiangzhong Zhao,Hua Wang
This article is dedicated to the preparation of a novel Chlorella protein hydrogel that can be used as a slow release carrier. Chlorella proteins and alginate were used as cross-linking agent at the present of 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide/ N- hydroxysuccinimide to obtain hydrogel with different 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide/ N- hydroxysuccinimide concentration. Textural results showed that hydrogel with high N- hydroxysuccinimide concentration possessed good hardness, springiness, gumminess and chewiness, espasically those hydrogel with 23% N- hydroxysuccinimide concentration. Scanning electron microscopy results revealed that the prepared hydrogel with higher N- hydroxysuccinimide concentration had better stable spatial porous structures. Swelling analyses indicated the prepared hydrogel exhibited pH sensitivity, which were beneficial for the slow-release. Then the hydrogels with 23% N- hydroxysuccinimide were used to be poly(lysine) slow-release carriers. Moreover, this hydrogel exhibited a prolonged and efficient period of poly(lysine) slow release at pH 1.2 (30%) and 7.4 (95%) within 200 h, which were further proved by good antibacterial activity against Escherichia coli and Staphylococcus aureus . Finally, based on the above results, we simulated the mechanism of hydrogel formation and poly(lysine) loading and slow release. The results reveal that the prepared Chlorella protein hydrogels are beneficial for use as slow-release carriers.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2022
·
IF 8.025
Caicai Li,Qian Zhang,Dongwei Lan,Mengyao Cai,Zulan Liu,Fangyin Dai,Lan Cheng
Many complex diseases, such as bacterial infections, frequently accompany cutaneous wound healing, adding to the difficulty of clinical wound management. Consequently, in addition to displaying strong biocompatibility and actively promoting wound healing, an optimal wound dressing should also possess antimicrobial qualities to address issues with bacterial infection. This paper developed natural silk fiber (SF) membranes (also known as a flat silk cocoon (FSC)) with antimicrobial properties as a dressing for skin wounds. By changing the spinning tools and environment of silkworm larvae, a novel natural SF membrane with a cocoon structure and controllable size was prepared. The functional SF membranes were obtained via a hot press process and grafted with ε -Poly- l -lysine (EPL). The results showed that the SF membrane dressing was adjustable in size with a similar structure to the extracellular matrix (ECM), displaying inherent mechanical properties, excellent antimicrobial qualities, and biocompatibility. In vivo experiments using a full-thickness skin defect model indicated that EPL-modified SF membranes significantly promoted the rate of wound healing, exhibiting thicker granulation tissue and higher collagen disposition than commercial dressings (Tegaderm™ film). Therefore, the excellent mechanical qualities and cytocompatibility of the antimicrobial EPL-modified SF membranes substantially promote their potential application as a chronic wound dressing.
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Inorganic Chemistry Frontiers
·
2022
·Vol. 9
·期 15
·3679-3691
·
IF 7.779
Lei Fan,Lianming Zhao,Yuchao Lv,Tinghu Wang,Yupeng Tian,Jianye Fu,Xinmei Liu
The synthesis of highly-dispersed non-noble metal based catalysts and their application in hydrogenation/dehydrogenation still remain challenging. Therefore, it is of great importance to protect metals from aggregation in both calcination and reduction processes. In this research, density functional theory (DFT) calculations were used to elucidate the stabilization effect of ionic liquid [Bmim][BF4] on Ni in the reduction process, showing that [Bmim][BF4] could efficiently inhibit Ni aggregation on the support. By further analysis combined with theoretical calculations, we found that the degree of metal dispersion before reduction directly determines the effect of [Bmim][BF4]. Guided by the DFT results, the method of co-assisted preparation of Ni/SAPO-11 using [Bmim][BF4] and lysine was adopted. Lysine forms a coordination compound with nickel oxides. The steric hindrance generated by the Ni–lysine complex can effectively inhibit the aggregation of nickel oxides during the calcination process. The DFT calculations revealed that during the reduction process, [BF4−] plays a dominant role in stabilization and electronic modulation by directly interacting with Ni on the support. The combination effects of [Bmim][BF4] and lysine effectively inhibit metal aggregation, leading to ultra-small nickel particles with a mean size of 4.4 nm over the obtained catalyst. Furthermore, the presence of [Bmim][BF4] also tunes the electronic state of Ni species. Using hexane isomerization as a probe reaction, the result indicates the excellent catalytic activity of the obtained catalyst (≈93.7% isomer selectivity at 63.5% conversion), much superior to that of Ni/SAPO-11 obtained from the traditional impregnation route (≈75.8% isomer selectivity at 64.6% conversion). This work provides a new method for the preparation of highly effective non-noble metal supported catalysts.
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APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
·
2019
·Vol. 188
·期 3
·769-786
·
IF 2.14
Li Chan,Zhang Wen,Yang Ning,Zhang Qing Song
As one of the typical phyllosilicate clays, hectorite (Hec) has some excellent characteristics and has been greatly applied in adsorption field for the removal of dye, endotoxin, etc. In this study, organic Hec nanocomposites modified with l -Lysine (Lys/Hec NCs) were prepared via solution intercalation method for BR removal. The effects of ionic strength, pH values, initial concentration of BR, and BSA concentration on the adsorption capacity for BR of Lys/Hec NCs were investigated. Results indicated that the adsorption capacity for BR of nanocomposites could reach 40 mg/g when the initial bilirubin concentration was 200 mg/L. However, the adsorption amount of Lys/Hec NCs decreased with increasing the concentration of BSA, but Lys/Hec NCs could still maintain a higher adsorption rate. The adsorption kinetics and adsorption isotherms indicated that the adsorption process of Lys/Hec NCs agreed well with the pseudo-second-order model and the Langmuir isotherm, respectively. Moreover, Lys/Hec NCs also exhibited excellent cytocompatibility. These obtained results demonstrate that Lys/Hec NCs prepared in this study had great potential to be used in hemoperfusion.
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RSC Advances
·
2024
·Vol. 14
·期 35
·25359-25368
·
IF 3.9
Yajun Jiang,Aolin Wu,Lin Yang,Jun Wu,Yue Liang,Zhigang Hu,Ying Wang
This study explored the preparation of a novel composite hydrogel based on deep eutectic solvent (DES) with lysine (Lys) and its application in pressure sensing and Fe3+ adsorption. DES was synthesized from acrylamide (AM) and urea (U) as hydrogen bond donors (HBD) with choline chloride (ChCl) as hydrogen bond acceptor (HBA), and Lys was used as a functional filler, and Lys/P(AM-U-ChCl) composite hydrogels were successfully prepared by frontal polymerization (FP) method. The structure of the hydrogels was characterized in depth using Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM). The effects of Lys content on the mechanical properties, pH-responsive behavior, pressure-sensitive properties, and Fe3+ adsorption capacity of the hydrogels were further analyzed. It was found that the introduction of Lys significantly improved the compressive and pressure-sensitive properties of the hydrogels. The composite hydrogels exhibited excellent swelling equilibrium rates at different pH values. The capacitance change of the hydrogel with 0.5 wt% Lys at 200 g pressure was 2.12-fold higher than that of the hydrogel without Lys addition, and the adsorption efficiency of the hydrogel for Fe3+ was greatly enhanced. This study provides a new idea for the functionalized design of composite hydrogels and demonstrates their great application prospects in high-performance pressure sensors and heavy metal ion adsorption.
研究领域:
材料科学
·
环境科学
·
生物医学工程
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Nano Today
·
2024
·
IF 13.2
Xingyou Wang,Xiaoqing Sun,Jiahui Lei,Linsen Li,Nanhang Zhu,Yue Yu,Yating Zeng,Ke Kang,QiangYing Yi,Yao Wu
Bacterial infected wounds pose a significant threat to patient survival, necessitating prompt and effective therapeutic intervention. Biomaterials that are responsive to wound microenvironments, possess well-defined structures and are facile to fabricate are favored for fulfilling their mission of efficient wound repair. Drawing inspiration from the immunoregulatory properties of natural zwitterionic capsular polysaccharides (ZPS) and glucomannan (GM) as well as the biogenesis of nitric oxide (NO), this study introduces the zwitterionic lysine glycopolymers (ZLGs), which harmoniously integrate the antibacterial, anti-fouling and immunoregulatory functions into one entity, to effectively promote the healing of infected wounds without addition of any external cytokines or molecular drugs. The ZLGs, exhibiting excellent cytocompatibility, restore sustained NO biogenesis (∼7.1 μM) in wound environment and thus to clean bacteria fast and effectively (12 h clearance rate for S. aureus : 97 %; E. coli : 92 %). Notably, the cell-membrane affinitive ZLGs induce efficient aggregation of mannose receptors and polarize the macrophages to M2 phenotype via the PI3K-Akt/STAT3 pathway, resulting in a synergistic enhanced polarization efficiency increasement of 105 %, as compare to the cumulation of individual building modules. The bioinspired ZLGs accelerate the repair of S. aureus infected wounds in vivo, highlighting the significance of this multi-in-one construction strategy for chronic wound treatment.
研究领域:
生物材料学
·
医学
·
免疫学
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SEPARATION AND PURIFICATION TECHNOLOGY
·
2024
·
IF 8.1
Feng Qi,Yangyang Li,Tanglei Zhang,Weiyu Dou,Riyue Shu,Zhikan Yao,Jing Wang,Lin Zhang
Segregating airborne particulate matters (PMs, especially PM 1.0 ) with high transmissibility of bacterial and viral pathogens is crucial for ensuring outdoor breathing safety. Electrospun nanofiber membranes (ENMs) have shown great promise for this purpose. However, the application of traditional ENMs is usually plagued by bacterial growth causing severe public health concern. Although some research has been done to overcome this challenge by doping antibacterial materials, the potential negative effects of these materials on the human body cannot be ignored. In this study, the ε-Poly-L-lysine (ε-PL), a green broad-spectrum antibacterial reagent, was grafted onto the surface of polydopamine (PDA) coated polyacrylonitrile (PAN) ENM via addition of Schiff base and Michael reaction. Experimental results indicated that the introduction of the ε-PL is key to the antibacterial performance of the membranes, and the antibacterial efficacy of E. coli and B. subtilis can reach to 99.86 % and 99.99 % respectively. Even after 7 days, the antibacterial efficiency of membrane remained at a high level. Additionally, the uniform structure of membranes endowed it with a high filtration efficiency of 80.9 % for PM 1.0 particles with a moderate transmembrane pressure drop (TMP) of 250 Pa and the corresponding quality factor is approximately 0.0066 Pa −1 . This study has far-reaching implications for the design of green antibacterial nanofibrous membranes to realize the segregation of harmful PMs.
研究领域:
材料科学
·
环境科学
·
生物学
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LWT-FOOD SCIENCE AND TECHNOLOGY
·
2020
·
IF 4.006
Qian Wu,Kuoquan Zhao,Yuanyuan Chen,Jinsong Xiao,Mengzhou Zhou,Dongshen Li,Nianjie Feng,Chao Wang
In this study, a glucose-lysine (G-L) model was selected to simulate the dietary system (cooking of alcohol-cured steak) to study the effect of alcohol concentration on the formation of advanced glycosylation products (AGEs) in Maillard reaction . It was first reported that higher alcohol concentrations accelerated the formation of AGEs as well as their intermediates glyoxal (GO) and methylglyoxal (MGO). The contents of GO and MGO increased from 11.74 ± 2.55 to 50.18 ± 0.16 μg/mL and 19.04 ± 1.25 to 58.51 ± 2.56 μg/mL with increasing ethanol concentration, respectively. High-alcohol samples produced higher levels of Nε-(carboxymethyl) lysine (CML) and Nɛ-carboxyethyl lysine (CEL) detected by U-HPLC-MS/MS. The capacity of catechin to inhibit the formation of AGEs and intermediates were significantly related to the concentration of ethanol, which may be due to ethanol accelerated catechin degradation and weakened the ability of catechin to trap GO and MGO. Our study strongly suggests that although we cannot completely avoid the AGEs produced by the Maillard reaction , in daily life, we try to avoid adding alcohol, especially high-concentration alcohol, when cooking and processing ingredients.
研究领域:
食品科学
·
化学
·
营养学
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Journal of Biomaterials and Tissue Engineering
·
2017
·Vol. 7
·期 10
·962-968
·
IF 1.383
Liu Tao,Li Xia,Liu Shihui,Tang Xiaohan,Wang Xin,Gong Tao,Hu Youdong,Ding Hongyan,Luo Xun,Pan Changjiang,Wang Qingmei
Thrombus and restenosis after stent implantation continue to be problematic. Surface modification by incorporating functional molecules to improve the biocompatibility of vascular stent has been a hot-topic in recent study. To date, however, researchers have not yet found the right
combination of surface physicochemical properties and architecture to meet clinical requirement. To investigate the influence of ordered structure and biofunction on platelets and vascular cells behavior, in this study, we construct three kinds of parallel heparin/poly-l-lysine nanoparticles
grooves (25/25 μm, 50/50 μm and 100/100 μm) on dopamine-coated 316L stainless steel surface by using micro-contact printing method. Fourier transform infrared and scanning electron microscopy results demonstrated that the nanoparticles were successfully immobilized
to dopamine-coated surface. Water contact angle and biomolecules quantitative characterization results suggested that three patterned groups were of similar hydrophilicity, as well as heparin and amine density. In vitro blood compatibility evaluation result indicated that surface patterned
with nanoparticles may selectively prevent platelet adhesion. Besides, the patterned area was found to inhibit endothelial cell adhesion. However, when culture medium contains high concentration of heparin-binding proteins, the endothelial cell growth profile on patterned area was greatly
improved.
研究领域:
生物材料学
·
血管生物学
·
医学工程
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CARBOHYDRATE POLYMERS
·
2015
·
IF 4.074
Ning Cui,Junmin Qian,Weijun Xu,Minghui Xu,Na Zhao,Ting Liu,Hongjie Wang
In the present study, poly( N ɛ -acryloyl- l -lysine)/hyaluronic acid (pLysAAm/HA) interpenetrating network (IPN) hydrogels were successfully fabricated through the combination of hydrazone bond crosslinking and photo-crosslinking reactions. The HA hydrogel network was first synthesized from 3,3′-dithiodipropionate hydrazide-modified HA and polyethylene glycol dilevulinate by hydrazone bond crosslinking. The pLysAAm hydrogel network was prepared from N ɛ -acryloyl- l -lysine and N , N ′-bis(acryloyl)-( l )-cystine by photo-crosslinking. The resultant pLysAAm/HA hydrogels had a good shape recovery property after loading and unloading for 1.5 cycles (up to 90%) and displayed a highly porous microstructure. Their compressive moduli were at least 5 times higher than that of HA hydrogels. The pLysAAm/HA hydrogels had an equilibrium swelling ratio of up to 37.9 and displayed a glutathione-responsive degradation behavior. The results from in vitro biocompatibility evaluation with pre-osteoblasts MC3T3-E1 cells revealed that the pLysAAm/HA hydrogels could support cell viability and proliferation. Hematoxylin and eosin staining indicated that the pLysAAm/HA hydrogels allowed cell and tissue infiltration, confirming their good in vivo biocompatibility. Therefore, the novel pLysAAm/HA IPN hydrogels have great potential for bone tissue engineering applications.
研究领域:
材料科学
·
生物医学工程
·
生物材料
·
组织工程
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TALANTA
·
2024
·
IF 5.6
Dandan Guo,Diya Zheng,Ding Zhou,Changlun Tong,Shaohua Huang
Lysine (Lys) is an essential nutrient that plays a crucial role in the growth and development of living organisms. Chiral analysis of Lys holds significant importance for ensuring the safety of food, pharmaceuticals, and health products. In this work, an achiral Zr-based metal organic frameworks (MOFs), UiO-66-NH 2 , was proposed as a fluorescent probe to achieve rapid response to l -lysine (L-Lys) in solution. Additionally, Zr 4+ in the skeleton of UiO-66-NH 2 framework exhibited different binding capacities towards Lys enantiomers, leading to distinct fluorescence responses for L-Lys and d -lysine (D-Lys). Leveraging these properties, the UiO-66-NH 2 probe enabled accurate determination of L-Lys concentrations in solution, as well as the enantiomeric excess (ee) values of Lys solutions. Notably, in the detection of Lys enantiomers, the achiral UiO-66-NH 2 acted as both a chiral selector and a fluorescent indicator, greatly improving the efficiency and stability of the detection system. The probable mechanism was further elucidated by pH titration experiments and density functional theory calculations. Additionally, the general applicability of this mechanism was validated by similar amino MOFs. The application of the UiO-66-NH 2 fluorescent probe in analysis of infant formula milk powders and liquid milk samples confirmed the reliability of the method. Moreover, the construction of fluorescence test paper by immobilizing UiO-66-NH 2 onto filler papers enabled the rapid identification of Lys enantiomers. Compared to previous fluorescent analyses of chiral amino acids assisted by additional metal ions, this study presented a novel approach and methodology that offers high efficiency, stability, reproducibility and reusability for the identification and detection of Lys enantiomers, highlighting the potential of the UiO-66-NH 2 fluorescent probe in advancing analytical techniques.
研究领域:
分析化学
·
生物化学
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