Materials
·
2025
·Vol. 18
·期 3
·478
·
IF 3.2
Jiayin Huang,Shiwang Xie,Haoqi Fan,Chen Song,Qiang Zheng,Dan Luo,Zhu Zeng,Zhou Li,Yujia Lv
In cell culture research and biotechnology, precise pH monitoring is crucial for maintaining cellular health and ensuring reliable experimental outcomes. Traditional pH measurement methods, such as glass electrodes and chemical indicators, are often limited by issues such as fragility, calibration requirements, and potential cytotoxicity. This study presents a novel pH sensor based on bacteriorhodopsin (bR), a light-sensitive protein that undergoes conformational changes in response to pH fluctuations, generating a measurable photoelectric signal. The integrated bR-based electrochemical electrode in a flexible pH biosensor is demonstrated, with measurements spanning the physiological pH range of 6.0–8.5. The sensor shows a high correlation (R2= 0.977) between photo-generated current signals and pH, indicating robust performance for real-time, non-invasive pH monitoring. The biocompatibility and non-invasive nature of this sensor make it particularly suitable for continuous monitoring in cell culture environments. The sensor’s practical application is validated by its integration into cell well plates for tracking the pH changes during cell growth, providing valuable insights into metabolic processes and growth conditions. In the future, efforts will focus on enhancing sensor sensitivity, stability, and integration with multi-parameter monitoring systems for more comprehensive cell culture analysis.
研究领域:
生物技术
·
细胞生物学
·
传感器技术
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产品关联
DOI
PubMed
高影响因子
NANO
·
2019
·
IF 1.293
Zongliang Kou,Detian Dou,Jiayi Zhu,Yinyin Mai,Herui Yi,Lihong Lan,Ping Lan
Amphiphilic polymer carriers (PEG–St– R ) were prepared from cassava starch and their pH response was investigated. First, hydrophobic tapioca starch polymer (St– R ) was prepared with octyl acyl as the hydrophobic group. The hydrophilic group polyethylene glycol (mPEG) was then introduced into the polymer by esterification to produce amphiphilic tapioca starch polymer (PEG–St– R ). Its self-assembly behavior was characterized using fluorescent probes. The morphology of PEG–St– R was investigated by transmission electron microscopy (TEM). Loading of the anti-cancer drug curcumin was used to assess the delivery and slow-release performance of the amphiphilic tapioca starch polymer. Cumulative drug release was explored at various pH conditions, with the greatest release from drug-loaded micelles being observed under acidic conditions and stable in a neutral environment. These results provide a theoretical basis for the preparation of pH-responsive nanomicelle carriers, and a platform for the preparation of novel amphiphilic starch-based polymers.
研究领域:
生物材料学
·
药剂学
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产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2021
·Vol. 22
·期 13
·7107
·
IF 5.924
Xiaosheng Tao,Fujian Jiang,Kang Cheng,Zhenzhen Qi,Vamsi K. Yadavalli,Shenzhou Lu
Silk fibroin (SF) has attracted much attention due to its high, tunable mechanical strength and excellent biocompatibility. Imparting the ability to respond to external stimuli can further enhance its scope of application. In order to imbue stimuli-responsive behavior in silk fibroin, we propose a new conjugated material, namely cationic SF (CSF) obtained by chemical modification of silk fibroin with ε-Poly-(L-lysine) (ε-PLL). This pH-responsive CSF hydrogel was prepared by enzymatic crosslinking using horseradish peroxidase and H2O2. Zeta potential measurements and SDS-PAGE gel electrophoresis show successful synthesis, with an increase in isoelectric point from 4.1 to 8.6. Fourier transform infrared (FTIR) and X-ray diffraction (XRD) results show that the modification does not affect the crystalline structure of SF. Most importantly, the synthesized CSF hydrogel has an excellent pH response. At 10 wt.% ε-PLL, a significant change in swelling with pH is observed. We further demonstrate that the hydrogel can be glucose-responsive by the addition of glucose oxidase (GOx). At high glucose concentration (400 mg/dL), the swelling of CSF/GOx hydrogel is as high as 345 ± 16%, while swelling in 200 mg/dL, 100 mg/dL and 0 mg/dL glucose solutions is 237 ± 12%, 163 ± 12% and 98 ± 15%, respectively. This shows the responsive swelling of CSF/GOx hydrogels to glucose, thus providing sufficient conditions for rapid drug release. Together with the versatility and biological properties of fibroin, such stimuli-responsive silk hydrogels have great potential in intelligent drug delivery, as soft matter substrates for enzymatic reactions and in other biomedical applications.Keywords:silk fibroin;hydrogel;ε-Poly-(L-lysine);pH-responsive;glucose-responsive
研究领域:
生物材料学
·
医学工程
·
药物递送系统
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产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2016
·
IF 4.758
Huayu Xiong,Wei Wang,Jichao Liang,Wei Wen,Xiuhua Zhang,Shengfu Wang
Herein, we report a convenient approach to purify water-soluble bovine serum albumin (BSA)-capped metal nanoclusters by pH-induced precipitation under acidic conditions. The fluorescence of metal nanoclusters could be completely recovered upon the re-dispersion of nanoparticles into basic media. BSA metal nanoclusters have been prepared to monitor pH at highly acidic medium. When pH decreased, the fluorescence of the nanoclusters under a UV lamp decreased and completely disappeared after pH 4. And a fluorescent pH sensor for pH monitoring has been developed based on the “OFF–ON” signal switch of the gold clusters agarose hydrogel.
研究领域:
材料科学
·
生物化学
·
分析化学
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DOI
高影响因子
APPLIED OPTICS
·
2023
·Vol. 62
·期 31
·8272-8278
·
IF 1.9
Zhenglan Bian,Caihong Dai,Fenghong Chu,Anduo Hu,Liang Xue,Qing Xu,Yuan Feng,Bin Zhou
This paper presents a hydrogel optical fiber fluorescence pH sensor doped with 5(6)-carboxyfluorescein (5(6)-FAM). The hydrogel optical fiber was fabricated with 2-hydroxy-2-methylpropiophenone as a photoinitiator, with different concentrations of polyethylene glycol diacrylate (PEGDA) for the core and cladding. A pH-sensitive fluorescence indicator 5(6)-FAM was doped into the core of the fiber. The prepared hydrogel optical fiber pH sensor showed good response within the pH range of 5.0–9.0. The linear range of the pH sensor is 6.0 to 8.0, with ${{R}^2} = {0.9904}$; within this range, the sensor shows good repeatability and reversibility, and the resolution is 0.07 pH units. The pHs of pork tissues soaked in different pH buffers were detected by the hydrogel optical fiber pH sensor; the linearity is 0.9828 when the pork tissue pH is in the range of 6.0–7.5. Due to the good ion permeability and biocompatibility of the hydrogel, this hydrogel optical fiber pH sensor is expected to be used in biomedical applications.
研究领域:
生物医学工程
·
传感技术
·
材料科学
✓ 已验证
DOI
PubMed
高影响因子
BIOMACROMOLECULES
·
2014
·
IF 5.788
Feng Liu,Shudong Lin,Zuoquan Zhang,Jiwen Hu,Guojun Liu,Yuanyuan Tu,Yang Yang,Hailiang Zou,Yangmiao Mo,Lei Miao
Three ternary graft copolymers bearing polystyrene (PS), poly(ethylene glycol) methyl ether (MPEG), and poly(acrylic acid) (PAA) side chains were synthesized and characterized. At pH = 7.4, these copolymers stabilized doxorubicin (DOX)-containing benzyl benzoate (BBZ) nanoemulsion droplets in water and formed a compact polymer layer to inhibit DOX release. Upon lowering the solution pH to 5.0, the AA groups dissociated less and became less soluble. Moreover, the neutralized AA groups formed presumably H-bonded complexes with the EG units, reducing the solubility of the EG units. This dual action drastically shifted the hydrophilic and hydrophobic balance of the copolymer and caused the original stabilizing polymer layer to rupture and the nanoemulsion droplets to aggregate, releasing DOX. The rate and extent of DOX release could be increased by matching the numbers of PAA and MPEG chains per graft copolymer. In addition, these nanoemulsions were not toxic and entered human carcinoma cells, releasing DOX there. Thus, these nanoemulsions have potential as drug delivery vehicles.
研究领域:
药剂学
·
聚合物化学
·
药物递送系统
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产品关联
DOI
PubMed
高影响因子
ACS Applied Polymer Materials
·
2024
·
IF 4.4
Zhonghua Lv,Xi Chen,Ling Yang,Mengting Li,Changjun Xia,Chunjia Luo,Min Chao,Luke Yan
We present a biomimetic switchable MXene membrane for water treatment inspired by chloride ion transmembrane transport. The MXene membrane was modified with poly(2-diethylaminoethyl methacrylate), resulting in increased hydrophilicity and enlarged channels. The membrane exhibited a permeability of 4.42 × 104 L m–2 h–1 bar–1, surpassing conventional MXene membranes. The membrane also separated Methylene Blue positively charged at pH = 12 and Amido Black 10B negatively charged at pH = 2 with separation efficiency above 98%, thanks to controllable channel size and adjustable electrostatic interactions in response to pH or CO2. This research offers valuable insights into the design of pH-responsive membranes with adjustable selectivity, representing a significant advancement in smart filtration with versatile applications.
研究领域:
材料科学
·
环境工程
·
生物仿生学
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产品关联
DOI
高影响因子
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2019
·Vol. 26
·期 24
·25107-25116
·
IF 2.914
Liu Guanghua,Lin Guanquan,Lin Xida,Zhou Hongjun,Chen Huayao,Hao Li,Zhou Xinhua
The overdosage use of pesticide was harmful to the environment and human health, which was mainly caused by the low utilization rate of the pesticide. However, the pesticide microcapsule with sustained-release and stimulating response properties could effectively solve this problem. Preparation of carboxymethyl cellulose grafting dimethyldiallylammonium chloride (CMC-g-PDMDAAC) through grafting polymerization and trapping as well as encapsulation of avermectin (AVM) via electrostatic interactions resulted in the formation of AVM/CMC-g-PDMDAAC microcapsules. The results showed that the particle size was 200~300 nm. The encapsulation efficiency was as high as 72.06%. Furthermore, the remaining rate of encapsulated AVM increased from 50.0 to 81.60% after UV irradiation for 359 min. The microcapsules exhibited significant enzyme and pH stimuli responsiveness. Finally, CMC-g-PDMDAAC had no significant difference effect on the toxicity of AVM, AVM could be found, and DMDAAC featured a synergistic effect on the toxicological effects of AVM.
Graphical abstract
研究领域:
农药科学
·
材料科学
·
环境科学
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产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2017
·
IF 6.32
Chen Shao,Jia Liang,Sihui He,Tianqi Luan,Jiantao Yu,Haoran Zhao,Jingyuan Xu,Leilei Tian
The interaction between graphene oxide (GO) and DNA is very sensitive to the environment. For example, under acidic conditions, the affinity of GO for DNA is enhanced, weakening the capability of GO to distinguish DNAs with different conformations. This effect has impeded the development of sensitive pH biosensors based on GO–DNA nanosystems. In this work, we systematically studied the affinity between GO and i-motif forming oligonucleotides (IFOs) at different pH values and developed a herring sperm DNA (HSD) treatment method. Using this method, HSD occupies the surface of GO, compromising the attractive force of GO that is significantly enhanced under acidic conditions. As a result, the ability of GO to distinguish between “open” and “closed” IFOs is successfully generalized to a wider pH range. Finally, a pH-sensitive GO–IFO nanosystem was fabricated that showed excellent sensing ability both in vitro and for intracellular pH detection. Because the interaction between GO and DNA is the basis for constructing GO–DNA biosensors, the strategy developed in this work shows great potential to be applied in a variety of other GO–DNA sensing systems.
研究领域:
生物化学
·
纳米生物学
·
分析化学
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产品关联
DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
·
2016
·
IF 7.145
Jie Mao,Yang Li,Tong Wu,Conghui Yuan,Birong Zeng,Yiting Xu,Lizong Dai
To precisely deliver drug molecules at a targeted site and in a controllable manner, there has been great interest in designing a synergistical drug delivery system that can achieve both surface charge-conversion and controlled release of a drug in response to different stimuli. Here we outline a simple method to construct an intelligent drug carrier, which can respond to two different pH values, therefore achieving charge conversion and chemical-bond-cleavage-induced drug release in a stepwise fashion. This drug carrier comes from the self-assembly of a block copolymer-DOX conjugate synthesized through a Schiff base reaction between poly(2-(diisopropylamino)ethyl methacrylate-b-poly(4-formylphenyl methacrylate-co-polyethylene glycol monomethyl ether methacrylate) (PDPA-b-P(FPMA-co-OEGMA)) and DOX. The surface charge of the BCP-DOX micelles reversed from negative to positive when encountering a weakly acidic environment due to the protonation of PDPA segments. In vitro cellular uptake measurement shows that the cellular uptake and internalization of the BCP-DOX micelles can be significantly enhanced at pH ∼ 6.5. Moreover, this drug carrier exhibits a pH-dependent drug release owing to the cleavage of the imine bond at pH < 5.5. With this dual-pH responsive feature, these micelles may have the ability to precisely deliver DOX to the cancer cells.
研究领域:
生物材料
·
药剂学
·
肿瘤学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF MOLECULAR LIQUIDS
·
2022
·
IF 6.633
Yanan Xing,Liya Zhang,Li Yu,Aixin Song,Junqing Hu
Stimuli-responsive Pickering foams have attracted wide attention due to their good stability and controllable foamability. In this work, pH-switchable Pickering foams were constructed using particles from amino acids with metal ions as stabilizers. Some hydrophobic amino acids, phenylalanine, leucine and tryptophan, can induce the production of particles with suitable wettability in the case of combination with some metal ions at their deprotonated forms, and further form Pickering foams. The foams exhibit good stability and can be cycled between foaming and defoaming forms by modulating pH of solutions. When pH was increased, the amino acids were in anionic forms and could easily coordinate with metal ions, forming insoluble particles to stabilize the foams. As pH was decreased, the protonation of amino acids led to the disappearance of particles, resulting in the defoaming state. This work proposes a simple method to prepare Pickering foams with environmentally friendly amino acids as the raw materials, providing a new idea for the construction of stimuli-responsive disperse systems.
研究领域:
材料科学
·
化学工程
·
胶体与界面科学
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产品关联
DOI
高影响因子
PHYSICAL CHEMISTRY CHEMICAL PHYSICS
·
2023
·Vol. 25
·期 37
·25780-25788
·
IF 3.3
Runna He,Shuangqing Sun,Jianpeng Cui,Mingshuo Chi,Zhikun Wang,Songqing Hu
In recent years, Pickering emulsifiers have been widely used in various production fields due to their excellent structural stability, biocompatibility and environmental friendliness. For some applications, it is required that the emulsifier can quickly respond to environmental stimuli and control the transition between stable and unstable emulsions. In this paper, we report a novel composite Pickering emulsifier with Fe3O4 as the core and magnetic response recognition body, silica as the intermediate protective layer, and chitosan (CS) of different molecular weights to endow solid particles with surface activity and pH-responsive properties. This emulsifier can stabilize the emulsion in the emulsion system with deionized water as the aqueous phase and liquid paraffin as the oil phase and can control the demulsification of the formed emulsion under the dual pH/magnetic stimulation. The experimental results show that Fe3O4@SiO2@CS has good paramagnetism and pH responsiveness. The particle size of the composite emulsifier nanoparticles is between 90 nm and 120 nm, and the best stabilizing effect of the emulsion is achieved when the dosage is 0.5 wt%. In the pH range of 3–11, the emulsifier can rapidly demulsify a stable paraffin oil–water emulsion system under the action of a magnetic field of strength 0.4 T. The pH response of the emulsifier is as follows: when pH ≤ 2, the system can form a stable emulsion, which is composed of fully protonated chitosan as a free chain segment and Fe3O4@SiO2. Emulsion stabilization was achieved with monolithic Fe3O4@SiO2@CS as an emulsifier at pH > 2, and demulsification was achieved at pH ≈ pKb (CS) at 298 K. The research in this paper can provide a feasible idea and synthesis method for the preparation of organic–inorganic composite structure emulsifier.
研究领域:
材料科学
·
化学工程
·
纳米技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）