Food Bioscience
·
2023
·
IF 5.2
Zhuojia Xu,Mengya Zhao,Qiwen Chen,Xinian Li,Zhenpeng Gao,Jing Guo,Fangyu Long
HHP was a nonthermal technology which was widely used in food processing. In this study, HHP was used to process the peanut sprout juice and microbial load , physicochemical properties and antioxidant capacities of peanut sprout juice were found to be improved during 90-day storage. Compared with heat treatment (HT, 95 °C, 10 min), the treatment at 400–600 MPa resulted in greater microbiological stability. No more than 2 lg CFU/g of total plate count (TPC) was observed at 600 MPa for 5 min during a 90-day storage period at 4 °C. The significant negative correlation between the total number of colonies and resveratrol was confirmed, which has bactericidal and bacteriostatic effects. Additionally, resveratrol treated with HHP would degrade and generate new degradation products like p -methylphenol, resorcinol, oxidized resveratrol with higher DPPH scavenging rate, which resulted the higher antioxidation capacity in HHP treated peanut sprout juice. In a word, this study focused on the effect of HHP on peanut sprout juice and the possible degradation behavior of the bioactive resveratrol with HHP treatment, providing a promising processing technology for peanut sprout juice with higher quality.
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2023
·Vol. 24
·期 13
·10914
·
IF 5.6
Minghui Cai,Haijuan Sun,Yujia Huang,Haixu Yao,Chen Zhao,Jiao Wang,Hui Zhu
Resveratrol performs a variety of biological activities, including the potential regulation of autophagy. However, it is unclear whether resveratrol protects against luteal dysfunction and whether autophagy involves the regulation of resveratrol. This study aims to investigate whether resveratrol can regulate autophagy to resist H2O2-induced luteinized granulosa cell dysfunction in vitro. Our results showed that resveratrol can enhance cell viability, stimulate the secretion of progesterone and estradiol, and resist cell apoptosis in H2O2-induced luteinized granulosa cell dysfunction. Resveratrol can activate autophagy by stimulating the expression of autophagy-related genes at the transcriptional and translational levels and increasing the formation of autophagosomes and autophagolysosomes. Rapamycin, 3-methyladenine, and bafilomycin A1 regulated the levels of autophagy-related genes in H2O2-induced luteinized granulosa cell dysfunction and further confirmed the protective role of autophagy activated by resveratrol. In conclusion, resveratrol activates autophagy to resist H2O2-induced oxidative dysfunction, which is crucial for stabilizing the secretory function of luteinized granulosa cells and inhibiting apoptosis. This study may contribute to revealing the protective effects of resveratrol on resisting luteal dysfunction from the perspective of regulating autophagy.Keywords:autophagy;resveratrol;corpus luteum;luteinized granulosa cell
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Foods
·
2023
·Vol. 12
·期 13
·2436
·
IF 5.2
Duoduo Li,Zihao Wei,Xiaolong Li
Hollow nanoparticles have attracted extensive attention due to their advantages such as high loading capacity and superior stability. However, the complexity of the preparation process and harmfulness of the used raw materials have limited their application in the food field. Based on this, hollow gliadin nanoparticles (HGNPs) were developed using a Na2CO3sacrificial template method. The findings of this study suggested that HGNPs could be regarded as a delivery system for resveratrol (Res) and they exhibited excellent delivery performance. Compared with solid gliadin nanoparticles (SGNPs), the HGNPs displayed smaller particle sizes, better physical stability, higher encapsulation efficiency, stronger resistance to ultraviolet light and a more sustained release of Res in the gastrointestinal tract. This work is of practical significance for the development and utilization of protein-based nanoparticles with hollow structures as a delivery system for sensitive bioactives.Keywords:gliadin nanoparticle;sacrificial template;hollow structure;resveratrol;delivery system;sustained release
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BMC Complementary Medicine and Therapies
·
2023
·Vol. 23
·期 1
·1-11
·
IF 3.9
Cai Weiye,Sun Bin,Song Chao,Liu Fei,Wu Zhengliang,Liu Zongchao
Background In mouse, it was discovered that resveratrol (Res) enhanced osteoporosis (OP) by boosting osteogenesis. Besides, Res can also have an impact on MC3T3-E1 cells, which are crucial for the control of osteogenesis and thus increase osteogenesis. Although some articles have discovered that Res enhanced autophagy to promote the value-added differentiation of MC3T3, it is unclear exactly how this affects the process of osteogenesis in mouse. Therefore, we will show that Res encourages MC3T3-E1 proliferation and differentiation in mouse pre-osteoblasts and further investigate the autophagy-related mechanism for this impact.Methods (1) MC3T3-E1 cells were separated into blank control group and various concentrations (0.01, 0.1, 1, 10, 100µmol/L) of group in order to determine the ideal Res concentration. In the Res group, Cell Counting Kit-8 (CCK-8) was used to measure the proliferation activity of pre-osteoblasts in mice in each group after resveratrol intervention. Alkaline Phosphatase (ALP) and alizarin red staining were used to gauge the degree of osteogenic differentiation, and RT-qPCR was used to measure the expression levels of Runx2 and OCN in the osteogenic differentiation ability of the cells. (2) In the experiment, four groups were set up: the control group, 3MA group, Res group, and Res + 3MA group. To examine cell mineralization, ALP and alizarin red staining were utilized. RT-qPCR and Western blot detection of cell autophagy activity levels and osteogenic differentiation capacity in each group following intervention.Results (1) Resveratrol might increase the number of mice pre-osteoblast, with the impact being most pronounced at 10µmol/L (P < 0.05). The nodules developed substantially more often than in the blank control group, and Runx2 and OCN expressions significantly increased (P < 0.05). (2) In contrast to the Res group, after 3MA purine blocked autophagy, the Res + 3MA group’s alkaline phosphatase staining and the development of mineralized nodules were reduced. Runx2, OCN, LC3II / LC3I expression decreased, p62 expression increased (P < 0.05).Conclusion The present study partially or indirectly demonstrated that Res may, through increased autophagy, induce osteogenic differentiation of MC3T3-E1 cells.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD HYDROCOLLOIDS
·
2018
·
IF 5.089
Lingtuo Zhang,Fang Zhang,Yapeng Fang,Shaoyun Wang
Utilization of protein-based nanoparticles encapsulating resveratrol is hampered by their instability against the environmental stress including pH, ionic strength and UV light. In this study, we fabricated a sodium alginate (Alg)-shelled soy protein isolate (SPI) nanoparticle for the encapsulation of resveratrol, abbreviated as RSAN. The aim of this study was to investigate the existence of Alg shell on both colloidal and chemical stabilities of RSAN. RSAN, fabricated at pH 3.5 and 0.25 mg/mL of Alg, is 204.5 ± 12.1 nm in diameter, −36.4 ± 1.2 mV in ζ-potential and 91.9 ± 3.3% in encapsulation efficiency of resveratrol. RSAN exhibited consistent solubility from pH 2.0 to 8.0 and was resistant to precipitation in ionic strength of 400 mM NaCl at various pHs. The protein in RSAN was prevented from digestion in gastric. Only 15% trans-resveratrol in RSAN was isomerized after 30 min UV light exposure, which is approximate 30% less than the non-shelled nanoparticle (RSN). Moreover, the encapsulation of resveratrol did not reduce its antioxidant activity . The findings suggest that this Alg-shelled nanoparticle has a potential in encapsulating hydrophobic bioactive compounds for utilization in functional foods.
✓ 已验证
产品关联
DOI
高影响因子
EUROPEAN POLYMER JOURNAL
·
2019
·
IF 3.621
Kan Zhang,Mengchao Han,Lu Han,Hatsuo Ishida
A series of thermally stable resveratrol-based tri-functional benzoxazine resins have been synthesized using resveratrol , different amines (aniline, 4-chloroaniline and 3-aminophenylacetylene) and paraformaldehyde. The molecular structures of these benzoxazines have been characterized by 1 H and 13 C NMR spectroscopy and FT-IR spectroscopy. The assignments for the characteristic protons and carbons in oxazine rings are confirmed using two-dimensional (2D) NMR techniques, including 1 H– 1 H NOESY and 1 H– 13 C HMQC. Moreover, the ring-opening polymerization of benzoxazines is studied using in situ FT-IR and differential scanning calorimetry (DSC), and the thermal stability and the flammability of the polybenzoxazines are also investigated by thermogravimetric analysis (TGA) and micro-scale combustion calorimetry (MCC), respectively. In addition to the advantages of lowest melting and polymerization temperatures amongst these resveratrol-based, tri-functional benzoxazines, the corresponding polybenzoxazine derived from benzoxazine containing acetylene shows highest thermal stability with a Tg temperature higher than 350 °C, initial degradation temperature at 10% weight loss at 465 °C, and char yield of 74% at 800 °C in nitrogen atmosphere. Furthermore, this polybenzoxazine also exhibits exceptionally low heat release capacity (30.7 J g −1 K −1 ) and total heat release value (6.0 kJ/g).
✓ 已验证
产品关联
DOI
高影响因子
Food & Function
·
2019
·Vol. 10
·期 6
·3291-3302
·
IF 3.241
Guoyan Ren,He Sun,Jinying Guo,Jinling Fan,Gen Li,Saiwen Xu
The molecular mechanism of the interaction between resveratrol and trypsin was studied using fluorescence spectroscopy (intrinsic fluorescence, synchronous fluorescence, three-dimensional fluorescence), ultraviolet-visible (UV-vis) spectroscopy, Fourier transform infrared (FT-IR) spectroscopy and the molecular docking method, as well as through enzyme activity and antioxidant assay. The fluorescence experiments (the Stern–Volmer quenching constants (KSV)) indicated that resveratrol quenched the intrinsic fluorescence of trypsin through the static quenching mechanism. The number of binding sites was about one. The thermodynamic functions ΔG < 0 and ΔH > 0, ΔS > 0 of the binding process, indicating that the combination of the resveratrol and trypsin processes was a spontaneous exothermic reaction and that the hydrophobic effect was the main force between them. UV-vis spectra, synchronous fluorescence spectra and three-dimensional fluorescence spectra analysis showed that the combination of resveratrol and trypsin induced changes in the microenvironment around the fluorophores of trypsin, resulting in alterations in the spatial structure of trypsin. FT-IR spectroscopy indicated that the contents of the α-helixes and β-turns in trypsin decreased and that the contents of the β-sheets, random coils and antiparallel β-sheets increased. All these experimental results were verified and reasonably explained by the molecular docking results. Upon resveratrol and trypsin binding, the enzyme catalytic activity of trypsin and the antioxidant of resveratrol decreased. Results from this study would be useful in elucidating the molecular mechanisms of the interactions between resveratrol and trypsin and contribute to making full use of resveratrol in the food industry.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CARBOHYDRATE POLYMERS
·
2017
·
IF 4.811
Jie Wu,Yaping Wang,Hao Yang,Xiangyu Liu,Zhong Lu
Nanoparticles with size range of 10–500 nm can be efficiently delivered into cancer cells by the Enhanced Permeability and Retention (EPR) effect. Here, we prepared resveratrol (Res) loaded chitosan (CS) nanoparticles with the size of 172–217 nm by an ionic cross-linking method, with sodium tripolyphosphate (TPP) as the cross-linking agent, to improve the stability, solubility and tumors targeting of the natural anti-cancer drug Res. The prepared Res loaded CS-TPP nanoparticles presented long-term storage stability and UV light stability. The cumulative drug release from nanoparticles in mimetic tumor tissue condition (pH 6.5) was higher than that in physiological condition (pH 7.4). Further, Res-loaded CS-TPP nanoparticles maintained the antioxidant activity of Res even after UV light irradiation. Cell viability study shows that the as prepared drug loaded nanoparticles had similar antiproliferative activity on hepatocellular carcinoma cells SMMC 7721 and lower cytotoxicity on normal hepatocyte cells L02 compared with free Res. Fluorescence microscopy observation revealed that the nanoparticles were efficiently taken in by SMMC 7721 cells. This work indicates the potential use of drug loaded CS-TPP nanoparticles for the efficient delivery of bioactive Res for chemotherapy.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY
·
2014
·Vol. 387
·期 11
·1079-1089
·
IF 2.36
Zhao Xin,Zhang Kai-ping,Huang Ting,Yan Cai-Chuan,Liu Li-Rong,Zhu Qi-Lei,Guo Feng-Feng,Liu Chen,Li Bao-Xin
Arsenic trioxide (As 2 O 3 ) is used to treat acute promyelocytic leukemia. However, the cardiotoxicity of long QT syndrome restricts its clinical application. Previous studies showed that As 2 O 3 can damage the human ether-a-go-go-related gene (hERG) current via disturbing its trafficking to cellular membrane. This study aimed to investigate whether the As 2 O 3 -insulted hERG channel can be rescued by resveratrol, a recognized cardioprotective agent. The whole-cell patch clamp technique was used to record the hERG current and action potential duration. Co-immunoprecipitation and Western blot assay were applied to determine the function of hERG-Hsp70/Hsp90 chaperone complexes and the expression alteration of protein-folding-related proteins, respectively. Compared with treatment of As 2 O 3 alone, co-treatment with resveratrol successfully restored the current and surface expression of hERG and obviously shortened action potential duration in guinea pig ventricular myocytes. Further experiments demonstrate that resveratrol relieved As 2 O 3 -caused endoplasmic reticulum (ER) stress by restoring the function of hERG-Hsp70/Hsp90 chaperone complexes and downregulating the protein expression of ER chaperone proteins (calnexin and calreticulin) and activating transcription factor 6. In conclusion, resveratrol was able to rescue the trafficking deficiency and relieve the ER stress (ERS). Our findings suggest that resveratrol has a potential effect to alleviate the adverse effect of As 2 O 3 on cardiotoxicity.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Microbiology Spectrum
·
2024
·
IF 3.7
You Zhou,Zhe Shen,Yan Xu,Xin-na Qian,Wei Chen,Jing Qiu
Bacterial biofilms are the major etiology agent of peri-implant disease. Chemical decontamination is a promising treatment strategy against bacterial biofilms; however, its applications are limited by its low efficiency and poor biocompatibility. In contrast to three conventional cleaners (sterile saline, hydrogen peroxide, and chlorhexidine), this study used resveratrol and naringin solutions to remove mature Staphylococcus aureus and Porphyromonas gingivalis biofilm on sandblasted (with large grit and acid-etched (SLA) titanium surface. To determine changes in surface characteristics, the surface wettability and roughness were measured, and micromorphology was observed by scanning electron microscopy. With crystal violet (CV) and live/dead bacterial staining, residual plaque quantity and composition were measured. The biocompatibility was tested using pH and cytotoxicity, as well as by osteoblasts (MC3T3-E1) adhesion, proliferation, and differentiation, and fibroblasts (L-929) proliferation were also analyzed. It was found that resveratrol and naringin solutions were more effective in restoring surface characteristics and also showed that less plaque and viable bacteria were left. Naringin removed S. aureus biofilms better than chlorhexidine. Alkaline resveratrol and naringin solutions increased cell adhesion, proliferation, and osteogenic differentiation without any cytotoxicity. Resveratrol increased the expression of mRNA and protein associated with osteogenesis. In conclusion, resveratrol and naringin effectively restored SLA titanium surface characteristics and decontaminated the biofilm with good biocompatibility, suggesting their therapeutic potential as chemical decontaminants.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Food Bioscience
·
2025
·
IF 5.9
Ruilu Sun,Wei Li,Minjie Liao,Yiran Yu,Yao Mou,Junfu Ji,Fang Chen,Xiaosong Hu,Lingjun Ma
Resveratrol (RES) and its catechol analog piceatannol (PIC), natural polyphenols, exhibit benefits in relieving glucose metabolism disorders. This study compared their effects on glucose metabolism regulation through in vivo and in vitro studies combined with quantitative analysis. Results showed that PIC significantly ameliorated insulin resistance and inhibited gluconeogenesis in obese mice, exhibiting superior efficacy to RES. Specifically, the area under the curve (AUC) of the intraperitoneal glucose tolerance test (IPGTT) was 2007.38 mmol・L −1 ・h for PIC, lower than 2450.88 mmol・L −1 ・h for RES; the phosphoenolpyruvate carboxykinase (PEPCK) concentration was 172.80 ng・L −1 in the PIC group, lower than 182.12 ng・L −1 in the RES group. At equimolar phenolic hydroxyl concentrations, PIC promoted greater glucose uptake and glycogen accumulation in human hepatocellular carcinoma (HepG2) cells compared to RES. Additionally, PIC accelerated tricarboxylic acid (TCA) cycle flux toward glucose utilization. These findings indicate that PIC is more effective than RES in regulating glucose metabolism, highlighting the critical role of the catechol moiety. This study provides guidance for screening natural bioactive polyphenols.
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF DRUG TARGETING
·
2024
·
IF 4.3
Jiaxin Liu,Liuqi Zhou,Huijing Cong,Jing Hu,Jingling Tang
Background Vaginal inflammation is a prevalent gynecological condition. If left untreated, it can potentially spread to the urinary and reproductive systems.Methods In this study, we propose a resveratrol-loaded microemulsion-based thermosensitive hydrogel (Res-Me-Tsgel) and compare it with a chitosan hydrogel-based Res-Me-Cogel. We characterized the different characters of Res-Me-Tsgel. The safety of Res-Me-Tsgel was also evaluated in vitro and in vivo. Finally, we measured the retention of Res in the vagina after drug administration.Results The Res-Me-Tsgel we prepared is a transparent liquid solution at room temperature that rapidly forms a gel at 37oC. Compared to Res solution and Res-Me, both Res-Me-Cogel and Res-Me-Tsgel demonstrate superior sustained release properties. Both in vitro and in vivo studies confirm the excellent biosafety profile of Res-Me-Cogel and Res-Me-Tsgel. Vaginal administration of these formulations in rats results in prolonged retention of resveratrol within the vagina. Notably, due to its improved flow into vaginal folds after administration, the retention of Resveratrol was approximately three times higher for the Res-Me-Tsgel group compared to the Res-Me-Cogel group at 24 h post-administration. Overall, these findings highlight the potential application of Res-Me-Tsgel as an effective means for vaginal inflammation.Conclusions We developed a novel micromulsion based thermosensitive hydrogel for the delivery of Res. The sustained release of Res and favorable vaginal retention from Res-Me-Tsgel make them promise as a potential candidate for local intravaginal therapy.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）