International Journal of Electrochemical Science
·
2023
·
IF 1.5
Jing Leng,Ping Li,Ling Bai,Yingqiong Peng,Yongfang Yu,Limin Lu
A Pd nanoparticles-graphene oxide (PdNPs/GO) hybrid as a highly sensitive sensing platform for determination of rutin was successfully prepared by a facile one-pot redox synthesis approach. Formation of PdNPs and loading onto the GO nanosheets were accomplished simultaneously by a redox reaction between H2PdCl4 and GO, without use of any additional reductants. Scanning electron microscopy (SEM) results demonstrated that PdNPs with an average size of 60 nm were distributed on the surface of GO nanosheets. Compared with the GO modified electrode, the PdNPs/GO modified electrode showed a better electrocatalytic activity toward the oxidation of rutin with larger peak current. Then the PdNPs/GO hybrid can be used as an enhanced sensing platform for the electrochemical determination of rutin. Under the optimal conditions, the anodic peak current was linear to the concentration of rutin in the range from 0.005 to 6 μM with the detection limit of 0.001 μM (S/N = 3). More importantly, the developed sensor can be used for the determination of rutin in medicine tablets with satisfactory results.
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JOURNAL OF CONTROLLED RELEASE
·
2026
·
IF 12.4
Feiyu Jin,Weicheng Shen,Mingxin Ren,Yaohan Guo,Yuanye Yan,Ziyang Ye,Yuewei Peng,Bochen Yao,Kai Dong
Excessive oxidative stress and neuroinflammation caused by retinal detachment (RD) drive progressive photoreceptor degeneration, posing a major challenge to functional vision recovery even after successful anatomical reattachment. These limitations highlight the urgent need for alternative or adjunctive therapeutic strategies to protect photoreceptors. Here, smart supramolecular Cerium–Rutin nanoparticles (CRNPs) are developed for coordinated redox and immunomodulatory therapy in RD. CRNPs are constructed through the coordination assembly of cerium ions with the natural flavonoid Rutin, integrating the reversible Ce 3+ /Ce 4+ redox-switching capacity with the intrinsic anti-inflammatory activity of Rutin. This supramolecular nanozyme platform enables dynamic regulation of oxidative and inflammatory homeostasis within the injured retina. In vitro, CRNPs protect microglial and photoreceptor cells by scavenging reactive oxygen species (ROS) and suppressing inflammatory activation, demonstrating both anti-inflammatory and cytoprotective effects. In vivo, CRNPs effectively preserve photoreceptor morphology, protect outer nuclear layer integrity, enhance retinal electrophysiological responses and vision function in an RD model. Mechanistically, CRNPs first mitigate oxidative stress, which in turn suppresses activation of the NLRP3/ASC/caspase-1 signaling pathway, thereby reducing pro-inflammatory microglial activation and limiting the release of inflammatory mediators. Collectively, this study establishes a redox-active nanozyme platform capable of restoring retinal homeostasis and mitigating neuroinflammation, providing a promising nanotherapeutic approach for the treatment of RD and other oxidative stress–associated neurodegenerative diseases.
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JOURNAL OF BIOMATERIALS APPLICATIONS
·
2023
·
IF 2.9
Mengyu Zhu,Yang Zhang,Chi Zhang,Lei Chen,Ying Kuang
Oxidative damage of neurons is one of the key pathological markers of Alzheimer’s disease (AD), which eventually leads to neuronal apoptosis and loss. Nuclear factor E2-related factor 2 (Nrf2) is a key regulator of antioxidant response and is considered to be an important therapeutic target for neurodegenerative diseases. In this study, the selenated derivative of antioxidant rutin (Se-Rutin) was synthesized with sodium selenate (Na2SeO3) as raw material by a simple electrostatic-compound in situ selenium reduction method. The effects of Se-Rutin on H2O2 induced oxidative damage in Pheochromocytoma PC12 cells were evaluated by cell viability, apoptosis, reactive oxygen species level and the expression of antioxidant response element (Nrf2). The results showed that H2O2 treatment significantly increased the level of apoptosis and reactive oxygen species, while the level of Nrf2 and HO-1 decreased. However, Se-Rutin significantly reduced H2O2 induced apoptosis and cytotoxicity, and increased the expression of Nrf2 and HO-1, both of which were better than that of pure rutin. Therefore, the activation of Nrf2/HO-1 signaling pathway may be the basis of Se-Rutin’s anti-oxidative damage to AD.
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高影响因子
MOLECULES
·
2022
·Vol. 27
·期 24
·8834
·
IF 4.927
Yue Huang,Xiaojing Si,Mei Han,Chen Bai
The aim of this study was to establish a rapid detection method of rutin in food based on nitrogen-doped carbon quantum dots (N-CDs) as the fluorescent probe. N-CDs were prepared via a single-step hydrothermal process using citric acid as the carbon source and thiourea as the nitrogen source. The optical properties of N-CDs were characterized using an electron transmission microscope, X-ray diffractometer, Fourier-transform infrared spectrometer, and nanoparticle size potential analyzer. The UV/Vis absorption property and fluorescence intensity of N-CDs were also characterized using the respective spectroscopy techniques. On this basis, the optimal conditions for the detection of rutin by N-CDs fluorescent probes were also explored. The synthesized N-CDs were amorphous carbon structures with good water solubility and optical properties, and the quantum yield was 24.1%. In phosphate-buffered solution at pH = 7.0, Rutin had a strong fluorescence-quenching effect on N-CDs, and the method showed good linearity (R2= 0.9996) when the concentration of Rutin was in the range of 0.1–400 μg/mL, with a detection limit of 0.033 μg/mL. The spiked recoveries in black buckwheat tea and wolfberry were in the range of 93.98–104.92%, the relative standard deviations (RSD) were in the range of 0.35–4.11%. The proposed method is simple, rapid, and sensitive, and it can be used for the rapid determination of rutin in food.Keywords:nitrogen-doped carbon quantum dots;hydrothermal synthesis;fluorescence probe;rutin
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CELL BIOLOGY INTERNATIONAL
·
2022
·Vol. 47
·期 3
·598-611
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IF 4.473
Yangyang Wu,Shuo Zhou,An Zhao,Yuling Mi,Caiqiao Zhang
Oxidative stress is a major cause of ovarian aging and follicular atresia. There is growing evidence that showed potential roles of rutin in antidiabetic, anti-inflammatory, antitumor, antibacterial and antioxidant, although it is yet unclear what the underlying mechanism is. Here, we looked into the potential effects of rutin on oxidative stress in the prehierarchical small white follicles (SWFs) from 580-day-old (D580) laying chickens. According to the findings, aging D580 layer ferroptosis was much higher than it was for laying hens during the peak period (280-day-old, D280). In both naturally aged and d -gal-induced chicken SWFs, rutin treatment concurrently boosted cell proliferation and prevented apoptosis. In addition, rutin inhibited the increased ferroptosis in aging hens. Meanwhile, rutin markedly suppressed the elevated ferroptosis and descending antioxidant capacity of D280-culture-SWFs from chicken elicited by d -gal. Rutin's activation of the Nrf2/HO-1 pathway hastened the SWFs' verbal battle with oxidative damage and reduced ferroptosis. Furthermore, activation of the ferroptosis signal increased the oxidative damage in SWFs. In conclusion, rutin alleviated oxidative stress that was induced by ferroptosis in aging chicken SWFs through Nrf2/HO-1 pathway. These findings point to a novel mechanism by which rutin protects SWFs from oxidative stress by suppressing ferroptosis, which is presumably a fresh approach to slowing ovarian aging in laying hens.
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FOOD CHEMISTRY
·
2022
·
IF 9.231
Lang Zhang,Yingying Hu,Qian Liu,Qian Chen,Xiufang Xia,Baohua Kong
This study aims to evaluate the heterocyclic aromatic amine (HAA) formation inhibited by cyanidin and rutin in chemical modeling systems and smoked chicken drumsticks. In the PhIP and β-carboline chemical modeling systems, cyanidin inhibited the PhIP, Harman, and Norharman formation while rutin inhibited the PhIP formation but promoted the Harman and Norharman formation compared with control sample ( P < 0.05). A mechanistic investigation confirmed that inhibiting the PhIP formation by cyanidin was mainly through trapping phenylalanine, creatine, creatinine, glucose, phenylacetaldehyde, and an aldol condensation product. In the smoked chicken drumsticks, cyanidin inhibited the PhIP, Harman, and Norharman formation dose-dependently compared to the control sample ( P < 0.05), with 2.0 % (w/v) cyanidin having the highest inhibitory effect. Moreover, cyanidin inhibited the formation of PhIP precursors and intermediates in the smoked chicken drumsticks. These results provide evidence for using phenolic compounds to reduce HAA formation in smoked meat products.
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高影响因子
MOLECULES
·
2023
·Vol. 28
·期 12
·4604
·
IF 4.6
Li Zhang,Mengting Zhang,Pingping Yang,Yin Zhang,Junjie Fei,Yixi Xie
Rutin, as a biological flavonoid glycoside, has very important medicinal value. The accurate and rapid detection of rutin is of great significance. Herein, an ultrasensitive electrochemical rutin sensor based on β-cyclodextrin metal–organic framework/reduced graphene oxide (β-CD-Ni-MOF-74/rGO) was constructed. The obtained β-CD-Ni-MOF-74 was characterized by X-ray diffraction spectroscopy (XRD), X-ray photoelectron spectroscopy (XPS), scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR), and nitrogen adsorption and desorption. The β-CD-Ni-MOF-74/rGO presented good electrochemical properties benefiting from the large specific surface area and good adsorption enrichment effect of β-CD-Ni-MOF-74 and the good conductivity of rGO. Under optimal conditions for the detection of rutin, the β-CD-Ni-MOF-74/rGO/GCE showed a wider linear range (0.06–1.0 μM) and lower detection limit (LOD, 0.68 nM, (S/N = 3)). Furthermore, the sensor shows good accuracy and stability for the detection of rutin in actual samples.Keywords:electroanalysis;carbon materials;electrochemical reduction;modified electrode
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JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
·
2023
·
IF 3
Wen Deng,Chengcheng Yang,Mengting Gong,Zhaohui Zhang,Hui Li
A [BMIM]PF 6 ion liquid (IL)-assisted synthesis of a rutin imprinted monolith (RIM) was carried out in an in-situ polymerization method. Bi-functional monomers and a ternary porogen containing IL was used for the RIM preparation and a L 9 (3 3 ) orthogonal factor design performed. Scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FT-IR) and N 2 adsorption method was for structural characterization of the RIMs. The monolith was directly used as stationary phase in liquid chromatography to test the retention selectivity, adsorption capacity and extraction application. The optimized porogen consists of 900 μL DMF, 144 μL ACN and 216 μL IL. The monolith RIM-13 obtained under the optimized conditions possessed improved adsorption performance, with a dynamic adsorption capacity of 6.695 mg/g, an imprinting efficiency of 4.841 and a selectivity α value of 4.821. Additionally, this monolith had also higher specific surface area , pore volume and permeability than that obtained without IL and the homogeneity of the imprint sites could be improved by using IL. When the RIM-13 was applied to the separation and purification of rutin from tartary buckwheat, a rutin product with a purity higher than 92 % can be obtained by one cycle. This molecular imprint solid-phase extraction (MI-SPE) is of potency to be applied to preparative-scale separation of other natural products.
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JOURNAL OF FOOD SCIENCE
·
2021
·Vol. 86
·期 5
·1714-1725
·
IF 3.167
Hai-Mei Li,Ting-Ting Xu,Qing-Xia Peng,Yong-Sheng Chen,Hua Zhou,Yu-Yun Lu,Ri-An Yan
Rutin (3′,4′,5,7-tetrahydroxy-flavone-3-rutinoside) was enzymatically acylated with benzoic acid and its esters (methyl benzoate and vinyl benzoate) using Thermomyces lanuginosus lipase (Lipozyme TLIM). The acylation reaction was optimized by varying the reaction medium, reaction temperature, acyl donor, substrate molar ratio, and reaction time. The highest conversion yield (76%) was obtained in tert -amyl alcohol (60 °C, 72 hr) using vinyl benzoate (molar ratio of 1:10) as acyl donor. The acylation occurred at the 2′′′-OH and 4′′′-OH of the rhamnose unit and the 2′′-OH position of the glucose moieties. Three novel rutin acylated derivatives (compounds 1 – 3 ) were purified and characterized by HR-MS and 1D and 2D NMR spectroscopy. We found that acylation significantly improved lipophilicity, capacity to inhibit lipid peroxidation, anticancer capacity and substantially maintained the antioxidant activity of rutin. This research provides important insights in the acylation of flavonoids with different glycosyl moieties. Practical Application In this study, three novel rutin derivatives were successfully synthesized and the highest conversion yield (76%) was obtained by reacting the rutin and vinyl benzoate at molar ratio of 1:10 in tert -amyl alcohol for 72 hr at 60 °C. Introducing a benzoic acid substituent into rutin molecule significantly improved their lipophilicity and inhibition of lipid peroxidation in lipophilic system. Furthermore, this study demonstrated that acylation significantly improved anticancer capacity and substantially maintained the antioxidant activity.
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FOOD CONTROL
·
2021
·
IF 5.548
Yan-mei Shi,Li-qin Chao,Lin Mei,Zhi-hong Chen,Xiu-min Li,Ming-san Miao
Herein, a soluble tetramine phthalocyanine indium-decorated reduced graphene oxide (rGO-InTAPc) nanocomposite was prepared via non-covalent functionalization and used to construct a novel electrochemical sensor for sensitive determination of rutin in Tartary buckwheat tea. The rGO-InTAPc as an improved electrode material can significantly increase the electroactive surface area and electron transfer capacity. Moreover, the electrochemical response can be dramatically expanded by the enrichment effect of rutin through the adsorption of π-π stacking. Under optimized conditions, the rGO-InTAPc sensor displayed a linear dependence for rutin concentration ranging from 0.005 to 100 μM with a detection limit of 2.0 nM ( S/N = 3). The proposed sensor with excellent selectivity and reproducibility was quantitatively validated in commercially available Tartary buckwheat tea with rutin level ranging of 9.06–13.87 mg/g, which results were highly consistent with these of HPLC. This strategy of rutin detection will address a long-standing analytical challenge to establishing facile and sensitive methods for quality monitoring in tea drinks.
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FOOD CHEMISTRY
·
2024
·
IF 8.5
Kaifeng Yang,Fangming Han,Yafeng Jin,Xiaobo Li
Herein, a stable and ultra-sensitive rutin electrochemical sensor was successfully developed. This sensor based on glassy carbon electrode (GCE) modified with C-GCS@ZIF-F/PL nanocomposite, which was made of thermally carbonized glucose (GCS) doped with flower-like ZIF (ZIF-F) and pencil lead (PL). The electrochemical response of rutin was considerably significant at C-GCS@ZIF-F/PL/GCE, demonstrating favorable conductivity and electrocatalytic properties for detection of rutin. Under optimal conditions, the linear range is 0.1–100 μM, with a low detection limit (LOD) of 0.0054 μM. It also exhibits excellent stability, reproducibility, as well as selectivity over common interfering ions such as Na + , uric acid, quercetin and riboflavin, etc. Meanwhile, the practical utility of developed sensor was evaluated in food samples including honey, orange, and buckwheat tea, achieving satisfactory recovery rates ranging from 98.2% to 101.7%. This paper introduces a novel technique for the detection of rutin in foods.
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Nutrients
·
2025
·Vol. 17
·期 10
·1734
·
IF 5
Yijing Ren,Lianyan Wang,Danyang Wang,Jian Huang,Ou Wang,Gangqiang Ding
Background: Rutin (R) is a bioactive compound with antioxidant and anti-inflammatory properties, but its low bioavailability limits its application. To address this problem, R was encapsulated with whey protein (W) as nanoparticles, and the potential effect and mechanism of rutin–whey protein nanoparticles (RW) on skeletal muscle dysfunction was investigated in D-galactose induced mice.Methods: R was encapsulated with W to form RW, and its characteristics like particle size, encapsulation efficiency, and bioaccessibility were evaluated. In the in vivo study, male C57BL/6J mice were treated with R, W or RW, respectively. The muscle function, hepatic antioxidant capacity, serum inflammatory levels, gut microbiota, and metabolomic profiles of mice were evaluated.Results: RW showed a uniform particle size, with an encapsulation efficiency of 68.7%. In the RW, the bioaccessibility of rutin was approximately 3.3 times that of free rutin. This in vivo study indicated that in comparison with D-galactose induced mice (model group), R, W and RW treatments could enhance hepatic antioxidative capacity and regulate inflammation levels, while W and RW could also increase muscle strength. Among these, RW treatment significantly elevated the hepatic GSH-PX activity and decreased the serum MSTN, TNF-α, and IL-6 levels, which were all markedly better than those of the individual effect of R or W. Such effects of R, W, and RW may be achieved through the modulation of gut microbiota that produced short-chain fatty acids or involved in anti-inflammatory function and the regulation of metabolic profiles associated with amino acid metabolism, aminoacyl-tRNA biosynthesis, etc.Conclusions: RW was found to enhance the bioaccessibility of rutin, and exhibited positive effects on skeletal muscle dysfunction via the modulation of gut microbiota and metabolic pathways. The results of this study may provide new scientific strategy for the utilization of rutin to achieve its health benefits.
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