Materials Today
·
2025
·
IF 22
Liang Li,Tao Yang,Kaixiang Ren,Xinchun Song,Shilin Wu,Hai-Wen Li,Zhipeng Jiang,Mengjun Sun,Yongtao Li
Lithium-LiNi 0.5 Mn 1.5 O 4 (LNMO) batteries are considered promising candidates for high-energy–density storage due to their high operational voltage and low cost. However, existing electrolytes typically fail to balance high-voltage stability with electrode compatibility, thereby limiting their performance under practical conditions. In this study, we present a synergistic inhibition engineering in which silyl additives—triethylsilyl trifluoromethanesulfonate (TESOTf) and tris(trimethylsiloxy)boron (TMSB)—are incorporated into a conventional carbonate electrolyte, leading to stable cycling of Li-LNMO batteries at voltages as high as 4.9 V. Through in situ techniques and theoretical simulations, we demonstrate that TMSB immobilizes free PF 6 − ions in the electrolyte, while TESOTf preferentially consumes trace amounts of water, thereby shifting the hydrolysis reaction pathway of PF 6 − and reducing side reactions at the cathode. Electrochemical tests show that the addition of small quantities of TESOTf and TMSB significantly enhances the rate capability (10C for 85.2 mAh g −1 ), cycling stability (5C for 500 cycles), and high-temperature performance (60 °C, 5C for 300 cycles) of Li-LNMO cells. More importantly, a practical 3 Ah Li-LNMO pouch cell containing these additives demonstrated stable cycling under practical conditions (20 mg cm −2 , 20 μm Li, 2 g Ah −1 ), achieving an energy density exceeding 300 Wh kg −1 .
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CHEMICAL RESEARCH IN CHINESE UNIVERSITIES
·
2024
·Vol. 40
·期 4
·730-736
·
IF 3.1
Hu Lin,Tong Shengrui,Xu Yanyong,Zhang Hailiang,Yu Shanshan,Chen Meifang,Ge Maofa
Unsaturated ketones are typical oxygenated volatile organic compounds (OVOCs) with high reactivity, and are important precursors in air pollution. The sources of OVOCs are complex and include direct emissions and secondary oxidation formation of VOCs in the atmosphere. 2-Cyclohexen-1-one is a widespread substance, and is derived from the industrial catalytic oxidation of cyclohexene. In this paper, we investigated the rate constants of the chemical reactions of 2-cyclohexen-1-one with NO 3 radicals, which is (7.25±0.29)×10 −15 cm 3 ·molecule −1 ·s −1 at 298 K and under 1 atm (1 atm=101325Pa). It supplemented the kinetics of NO 3 radicals database, and revealed its effects in the nighttime atmosphere. In addition, the reaction products of 2-cyclohexen-1-one with NO 3 radicals were detected by Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS), which revealed a series of nitrate esters in the composition of the secondary organic aerosol (SOA), which may reduce atmospheric visibility. Finally, the possible pathways for the generation of the products were developed.
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Foods
·
2024
·Vol. 13
·期 9
·1338
·
IF 4.7
Ruimin Teng,Cun Ao,Haitao Huang,Daliang Shi,Yuxiao Mao,Xuxia Zheng,Yun Zhao
Longjing tea is favored by consumers due to its refreshing and delicate aroma, as well as its fresh and sweet flavor. In order to study the processing technology of Longjing tea with ‘Baiye 1’ tea varieties, solid phase microextraction and gas chromatography–mass spectrometry were used to analyze the volatile components of Longjing tea in different process stages. The results revealed the identification of 275 aroma metabolites in the processing samples of Longjing tea. The sensory evaluation and principal component analysis revealed that the leaves of fresh (XY) and spreading (TF) were different from the leaves of first panning (YQ), second panning (EQ), final panning (HG), and fragrance enhancing (TX). The relative contents of geraniol (1199.95 and 1134.51), linalool (745.93 and 793.98), methyl salicylate (485.22 and 314.67), phenylethyl alcohol (280.14 and 393.98), 2-methylfuran (872.28 and 517.96), 2-butenal (56.01 and 154.60), and 2-hexenal (46.22 and 42.24), refreshing and floral substances in the XY and TF stages, were higher than other stages. The aroma contents of 2-methylfuran, furfural, 2-methyl-1-penten-3-one, 3-hexen-2-one, dodecane, hexanoyl hexanoate, 2,5-dimethyl-pyrazine, and methyl-pyrazine were found to be significantly positively correlated with the intensity of chestnut aroma. In conclusion, this study contributes to a better understanding of the composition and formation mechanism of chestnut-like aroma and provides new insights into the processing technology to improve the quality of albino green tea.
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PubMed
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AMB Express
·
2024
·Vol. 14
·期 1
·1-16
·
IF 3.5
Yan Yan,Yuan Haisheng,Yang Fan,Na Heiya,Yu Xiuling,Liu Jingran,Wang Yuzhen
Non-alcoholic fatty liver disease (NAFLD) is becoming a significant global public health threat. Seabuckthorn ( Hippophae rhamnoides L.) has been used in traditional Chinese medicine (TCM). The hypolipidemic effects of Seabuckthorn polysaccharides (SP) against high-fat diets (HFD)-induced NAFLD were systematically explored and compared with that of Bifidobacterium lactis V9 ( B. Lactis V9). Results showed that HFD-induced alanine transaminase (ALT) and aspartate aminotransferase (AST) levels decreased by 2.8-fold and 4.5-fold, respectively, after SP supplementation. Moreover, the alleviating effect on hepatic lipid accumulation is better than that of B. Lactis V9. The ACC and FASN mRNA levels were significantly reduced by 1.8 fold ( P < 0.05) and 2.3 folds ( P < 0.05), respectively, while the CPT1α and PPARα mRNA levels was significantly increased by 2.3 fold ( P < 0.05) and 1.6 fold ( P < 0.05), respectively, after SP administration. SP activated phosphorylated-AMPK and inhibited PPARγ protein expression, improved serum oxidative stress and inflammation ( P < 0.05). SP supplementation leads to increased hepatic expression of nuclear factor erythroid 2-related factor 2 (Nrf-2), heme oxygenase-1 (HO-1) and Superoxide dismutase-2 (SOD-2). Furthermore, SP treatment improved HFD-induced intestinal dysbiosis. Lentisphaerae , Firmicutes , Tenericutes and Peptococcus sp., RC9_gut_group sp., and Parabacteroides sp. of the gut microbiota were significantly associated with hepatic steatosis and indicators related to oxidative stress and inflammation. Therefore, SP can mitigate hepatic lipid accumulation by regulating Nrf-2/HO-1 signaling pathways and gut microbiota. This study offers new evidence supporting the use of SP as a prebiotic treatment for NAFLD.
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Cell Reports
·
2024
·
IF 7.5
Xueping Li,Jiarui Zhou,Xingmei Liu,Chen Jin,Le Liu,Hongmin Sun,Qian Wang,Qiushi Wang,Ruiying Liu,Xiaoyu Zheng,Yutao Liu,Yu Pang
Uropathogenic Escherichia coli (UPEC) is the most common causative agent of urinary tract infection (UTI). UPEC invades bladder epithelial cells (BECs) via fusiform vesicles, escapes into the cytosol, and establishes biofilm-like intracellular bacterial communities (IBCs). Nucleoside-diphosphate kinase (NDK) is secreted by pathogenic bacteria to enhance virulence. However, whether NDK is involved in UPEC pathogenesis remains unclear. Here, we find that the lack of ndk impairs the colonization of UPEC CFT073 in mouse bladders and kidneys owing to the impaired ability of UPEC to form IBCs. Furthermore, we demonstrate that NDK inhibits caspase-1-dependent pyroptosis by consuming extracellular ATP, preventing superficial BEC exfoliation, and promoting IBC formation. UPEC utilizes the reactive oxygen species (ROS) sensor OxyR to indirectly activate the regulator integration host factor, which then directly activates ndk expression in response to intracellular ROS. Here, we reveal a signaling transduction pathway that UPEC employs to inhibit superficial BEC exfoliation, thus facilitating acute UTI.
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MICROPOROUS AND MESOPOROUS MATERIALS
·
2024
·
IF 4.8
Yuqing Ouyang,Ningning Du,Xiaoyun Xiong,Qingxun Hu,Hongman Sun,Tingting Li,Jie Zhong,Youhe Wang,Zifeng Yan
The combination of titanium silicalite-1 (TS-1) zeolite and H 2 O 2 oxidant provides an excellent alternative to many highly toxic and polluting olefin oxidation processes due to the by-product being water-only and without deep oxidation. However, the diffusion of bulky reactants and products suffers from the inherent micropores of conventional TS-1 zeolites. Herein, we designed a reticulated ordered macroporous carbon material with uniform and coherent mesopores, and successfully synthesized ordered hierarchical TS-1 zeolites containing intracrystalline macroporous-mesoporous-microporous by dry gel method. The fully interconnected intracrystalline macroporous-mesoporous-microporous hierarchical TS-1 improves the accessibility of the active site and the diffusion efficiency of bulky compounds. It showed excellent catalytic performance during the epoxidation reaction of chloropropene, and its epichlorohydrin yield and H 2 O 2 effective utilization were 70.11% and 77.05%, which were 2.2 and 1.9 times of the catalytic performance of commercial TS-1 zeolites, respectively, and significantly better than the ultrafine TS-1 synthesized by conventional methods. The high catalytic performance was maintained after three cycles of use. This offers a good opportunity for the design and synthesis of efficient zeolite catalysts in the future.
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AICHE JOURNAL
·
2025
·
IF 4
Yulin Li,Qian Liu,Ke Wang,Kunchi Xie,Xiangyi Kong,Zhen Song,Zhiwen Qi,Hongye Cheng
Efficient separation of high water-content azeotropes such as 1-methoxy-2-propanol (PGME) and water is crucial but challenging. Herein, a green and low-energy hybrid extraction-distillation process was developed using dibutyl oxalate (DBO) as the extractant. Experimental evaluations revealed the superior performance of DBO with high selectivity and minimal solvent loss at various PGME concentrations. Mechanistic studies via molecular simulations and FT-IR analyses demonstrated that hydrogen bonding between the carbonyl group of DBO and the hydroxyl group of PGME drives the selective extraction. Process simulation indicated a 37.0% reduction in solvent circulation, 52.6% in energy consumption, and 57.9% in carbon footprint compared to conventional benzene-based azeotropic distillation. Life cycle assessment further confirmed the environmental benefits of DBO, highlighting its low toxicity, biodegradability, and renewable synthesis potential. This work presents a sustainable and cost-effective strategy not only for PGME-water separation but also for other high water-content azeotropes separation.
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JCI Insight
·
2021
·
IF 8.315
Tonghui Xu,Jialin Guo,Maozeng Wei,Jiali Wang,Kehui Yang,Chang Pan,Jiaojiao Pang,Li Xue,Qiuhuan Yuan,Mengyang Xue,Jian Zhang,Wentao Sang,Tangxing Jiang,Yuguo Chen,Feng Xu
The mitochondrial enzyme aldehyde dehydrogenase 2 (ALDH2) catalyzes the detoxification of acetaldehyde and endogenous lipid aldehydes. Approximately 40% of East Asians, accounting for 8% of the human population, carry the E504K mutation in ALDH2 that leads to accumulation of toxic reactive aldehydes and increases the risk for cardiovascular disease, cancer, and Alzheimer disease, among others. However, the role of ALDH2 in acute kidney injury (AKI) remains poorly defined and is therefore the subject of the present study using various cellular and organismal sources. In murine models, in which AKI was induced by either the contrast agent iohexol or renal ischemia/reperfusion, KO, activation/overexpression of ALDH2 were associated with increased and decreased renal injury, respectively. In murine renal tubular epithelial cells (RTECs), ALDH2 upregulated Beclin-1 expression, promoted autophagy activation, and eliminated ROS. In vivo and in vitro, both 3-MA and Beclin-1 siRNAs inhibited autophagy and abolished ALDH2-mediated renoprotection. In mice with iohexol-induced AKI, ALDH2 knockdown in RTECs using AAV-shRNA impaired autophagy activation and aggravated renal injury. In human renal proximal tubular epithelial HK-2 cells exposed to iohexol, ALDH2 activation potentiated autophagy and attenuated apoptosis. In mice with AKI induced by renal ischemia/reperfusion, ALDH2 overexpression or pretreatment regulated autophagy mitigating apoptosis of RTECs and renal injury. In summary, our data collectively substantiate a critical role of ALDH2 in AKI via autophagy activation involving the Beclin-1 pathway.
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ACTA PHARMACOLOGICA SINICA
·
2020
·Vol. 41
·期 11
·1465-1475
·
IF 5.064
Liang Li-min,Zhou Jun-jie,Xu Feng,Liu Pei-hua,Qin Lan,Liu Li,Liu Xiao-dong
Peptide transporter 1 (PepT1), highly expressed on the apical membrane of enterocytes, is involved in energy balance and mediates intestinal absorption of peptidomimetic drugs. In this study, we investigated whether and how diabetes affected the function and expression of intestinal PepT1. Diabetes was induced in rats by combination of high-fat diet and low dose streptozocin injection. Pharmacokinetics study demonstrated that diabetes significantly decreased plasma exposures of cephalexin and acyclovir following oral administration of cephalexin and valacyclovir, respectively. Single-pass intestinal perfusion analysis showed that diabetes remarkably decreased cephalexin absorption, which was associated with decreased expression of intestinal PepT1 protein. We assessed the levels of bile acids in intestine of diabetic rats, and found that diabetic rats exhibited significantly higher levels of chenodeoxycholic acid (CDCA), cholic acid (CA) and glycocholic acid (GCA), and lower levels of lithocholic acid (LCA) and hyodeoxycholic acid (HDCA) than control rats; intestinal deoxycholic acid (DCA) levels were unaltered. In Caco-2 cells, the 6 bile acids remarkably decreased expression of PepT1 protein with CDCA causing the strongest inhibition, whereas TNF-α, LPS and insulin little affected expression of PepT1 protein; short-chain fatty acids induced rather than decreased expression of PepT1 protein. Farnesoid X receptor (FXR) inhibitor glycine-β-muricholic acid or FXR knockdown reversed the downregulation of PepT1 expression by CDCA and GW4064 (another FXR agonist). In diabetic rats, the expression of intestinal FXR protein was markedly increased. Oral administration of CDCA (90, 180 mg·kg −1 ·d −1 , for 3 weeks) dose-dependently decreased the expression and function of intestinal PepT1 in rats. In conclusion, diabetes impairs the expression and function of intestinal PepT1 partly via CDCA-mediated FXR activation.
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ANALYTICAL BIOCHEMISTRY
·
2021
·
IF 3.365
Haiyan Zhou,Guorui Zhong,Jing Bai,Xiaolei Li,Wen Peng,Lei Zhang,Jing Li
The urate transporter 1 (URAT1) inhibitors were considered a very promising class of uricosuric agents for the treatment of hyperuricemia and gout. In vitro activity testing of these compounds has been conducted by radio-labeling uric acid for a long time. However, relatively few offer the convenience and speed of fluorescence-based assays. Herein, we report the development of a non-radioactive cell-based method for the screening of URAT1 inhibitors using the human embryonic kidney 293T cells stably expressing human URAT1, and 6-carboxyfluorescein (6-CFL) as a substrate. The URAT1-mediated transport of 6-CFL was time dependent and saturable (Km = 239.5 μM, Vmax = 6.2 pmol/well/min, respectively). Molecules known to interact with organic anion transporters, including benzbromarone , probenecid , and lesinurad, demonstrated concentration-dependent inhibition of 6-CFL transport by URAT1. Moreover, we screened a small subset of compounds, and identified compound 4 as a promising URAT1 inhibitor. This in vitro assay may be employed to screen for novel URAT1 inhibitors, which are effective against hyperuricemia.
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Foods
·
2022
·Vol. 11
·期 19
·2978
·
IF 5.561
Wenyan Lin,Yaping Liu,Jianbing Di,Gang Ren,Wei Wang,Weichun He,Yu Wang
Root mustard is plentiful in vitamins and minerals but shrivels and molds easily. In this study, freshly harvested root mustard was fumigated with various concentrations of 1-Methycyclopropene(1-MCP) (1 µL L−1, 1.5 µL L−1, and 2.0 µL L−1) for 24 h and stored at ambient temperature (17 ± 1 °C) for 35 d. Our data showed that 1-MCP fumigation had a striking preservation effect on maintaining weight loss, fruit firmness, lignin, Vc content, and moisture content, inhibiting respiratory intensity and ethylene release rate, as well as decreasing cell permeability and malondialdehyde (MDA) accumulation and maintaining cell membrane integrity of root mustard. In addition, lipoxygenase (LOX), pyruvate dehydrogenase (PDH), and polyphenol oxidase (PPO) activities were significantly reduced throughout the storage period. In contrast, the activities of succinate dehydrogenase (SDH), superoxide dismutase (SOD), ascorbate peroxidase (APX), phenylalanine deaminase (PAL), and peroxidase (POD) remained at high levels. Results showed that 1-MCP treatments were effective in maintaining the quality of root mustard, and the preservation effect of 1.0 μL·L−11-MCP was better than other concentrations of 1-MCP. This study could serve as a theoretical reference for root mustard preservation.Keywords:1-MCP;root mustard;physiology;storage quality
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MEDICAL SCIENCE MONITOR
·
2018
·
IF 1.894
Shishan Xiao,Hongqian Zhu
Background Leukemia cells have strong proliferation and anti-apoptosis capabilities. The purpose of this study was to investigate the effect of silencing the leucine-rich alpha-2-glycoprotein1 (LRG1) gene, which was found to regulate tumor proliferation and apoptosis in acute myeloid leukemia (AML) cell lines. Material/Methods Plasmid interference technique was used to silence the LRG1 gene in the KASUMI-1 cell line. The cell counting kit-8 (CCK-8) assay was used to test the effect of transduction on cell viability. Cell cycle and apoptosis were detected by flow cytometry. Western blot and quantitative real-time polymerase chain reaction (RT-qPCR) were applied to detect the expression levels of proteins and mRNA, respectively. Results KASUMI-1 cells with the CD34 + CD38 − phenotype were sorted by flow cytometry. After transfection of the siLRG1 plasmid, the level of LRG1 expression was downregulated and cell viability was reduced. Silencing of LRG1 gene blocked KASUMI-1 cells in G0/G1 phase and promoted apoptosis. Further experiments found that LRG1 gene silencing significantly downregulated cell cycle-associated proteins and anti-apoptotic proteins, while upregulating pro-apoptotic proteins. Downregulation of LRG1 gene expression also inhibits signal transduction of the JAK-STAT pathway. Conclusions LRG1 gene silencing regulates the expression of cyclin and apoptosis-related proteins to reduce cell viability and promote apoptosis, probably through inhibition of the JAK-STAT pathway.
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