ACS Synthetic Biology
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2025
·
IF 3.9
Jerome R. Lon,Xuemei Zhao,Gulkiz Mamatrixat,Zhoukang Zhuang,Zhehao Jin,Tao Yu,Jufang Wang,Hongting Tang
Terpenoids are a diverse class of compounds with significant application potential. While prokaryotic bacteria synthesize terpenoids via the methylerythritol-4-phosphate (MEP) pathway, fungi utilize the mevalonate (MVA) pathway. The MVA pathway has been widely employed for efficient terpenoid production in bacteria such as Escherichia coli, but the MEP pathway performs poorly for biosynthesis in yeast. In this study, we constructed a compartmentalized MEP pathway to enhance monoterpenoid production in Saccharomyces cerevisiae. By introducing a geraniol synthase, we initially achieved the production of geraniol from glucose. Further effective incorporation of a cytosolic MEP pathway with nine enzymes increased geraniol production by 174.5%. However, this also significantly inhibited cell growth. Overexpression analysis revealed that flavodoxin and flavodoxin reductase were major contributors to growth inhibition, which could also be a factor limiting the application of the MEP pathway. To address these issues, we employed peroxisomal compartmentalization to isolate the MEP pathway from cytosolic metabolism. This strategy alleviated growth inhibition and improved geraniol production by 93.18% compared to that of cytosolic expression. Through additional metabolic engineering, we optimized peroxisomal geraniol production, achieving a yield of 30.64 mg/L. Our findings demonstrate the potential of compartmentalized MEP pathway expression as a viable approach for enhancing terpenoid biosynthesis in yeast, offering valuable insights for future metabolic engineering efforts.
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CHEMICAL ENGINEERING JOURNAL
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2025
·
IF 13.2
Hanhan Zhao,Wenjun Jiang,Yinhua Ma,Liubin Pei,Jiayu An,Su Zhan,Feng Zhou
The inherent electron-hole recombination, low light-absorption capacity and poor selectivity of photocatalysts hinder the production efficiency of hydrogen peroxide (H 2 O 2 ), necessitating the development of a catalyst with high catalytic activity. In this study, a supramolecular self-assembly method was employed to prepare 4-aminobenzonitrile-modified carbon nitride (Abz-CN). The incorporation of the cyano group rendered the charge distribution of Abz-CN inhomogeneous, leading to an increased dipole moment. This enhancement facilitated the separation and migration of photoinduced carriers. Additionally, density functional theory (DFT) calculations revealed that Abz-CN had a higher propensity for oxygen adsorption and the formation of *OOH intermediates compared to carbon nitride (BCN). The H 2 O 2 generation rate of Abz-CN reached 3866 μmol·g −1 ·h −1 (under a 180 W xenon lamp, λ > 400 nm), which was 5.2 times that of BCN. Furthermore, the mechanism of Abz-CN followed a sequential two-step single-electron oxygen reduction process. This study showcases an innovative approach for bolstering the efficiency of photocatalytic H 2 O 2 generation.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2025
·
IF 6.2
Man Cai,Zheng Xing,Chen Du,Xinyu Ding,Mengyu Zhang,Chengjin Zhou
The ecological risks brought by heavy metals and 4-monobromodiphenyl ether (BDE-3) copollutants are among the most common environmental contaminants. Heavy metals and other substrates are actively transported across cellular membranes by an ATP-binding cassette (ABC) transporter. Previous studies have suggested that phyllospheric yeast (Wickerhamomyces anomalus) can survive in BDE-3 and heavy metal environments. However, the underlying molecular mechanisms of BDE-3 and heavy metal tolerance are not clear. To assess the impact of the yeast transporter pdr15 gene on plant tolerance, tissue heavy metal levels, and BDE-3 levels, we heterologously expressed the gene in tobacco. Accumulating evidence has suggested that transgenic tobacco contributes to the reduction of plant damage by resulting in heavy metals and BDE-3 in specific subcellular organelles. Dimethyl ether and phthalic acid metabolism were the two main metabolic pathways for the toxicity-reducing effect. Our findings broaden the understanding of ABC transporter function and their regulatory metabolic pathways in heavy metal and BDE-3 co-contaminated resistance.
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JOURNAL OF MOLECULAR STRUCTURE
·
2025
·
IF 4.7
Dantong Wang,Hanqing Xie,Yongqi Zeng,Fengxi Li,Lei Wang,Peng Chen,Zhi Wang
This study presented, for the first time, a method for synthesizing 4-amino-3,5-dicyano-6-aryl phthalates using porcine pancreatic lipase (PPL) as a catalyst. We synthesized 18 derivatives of 4-amino-3,5-dicyano-6-aryl phthalates employing aryl aldehyde, acetylenic esters, and malononitrile under optimal conditions for 4 h, with water serving as the primary reaction medium. The yields of the target products ranged from moderate to high, achieving 43 % to 95 %. This method demonstrates an excellent tolerance for various functional groups. Furthermore, this paper elucidates the reaction mechanism through molecular docking studies and confirms the interaction between the substrate and lipase. The data indicate that the measured distance from the substrate to the catalytic triad is 3.8 Å, with a binding energy of -4.6 kJ/mol, suggesting that PPL demonstrates the strongest catalytic potential in this specific reaction. Additionally, the aforementioned products underwent molecular docking analysis with three selected enzyme targets from Leishmania, highlighting their potential as lead compounds for anti-Leishmania activity.
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Desalination and Water Treatment
·
2024
·
IF 1
Youning Chen,Min Wang,Shuang Jia,Yuhong Li,Xiaohua Meng,Xiaoling Yang
ABSTRACT A novel poly(4-styrene sulfonic acid)-graft resin (PSS-g-PS) with high adsorption capacity was prepared by grafting poly(4-styrene sulfonic acid) (PSS) onto chloromethylated styrene resin via surface-initiated atom transfer radical polymerization. The surface composition of the poly(4-styrene sulfonic acid)-grafted resin were characterized by Fourier-transform infrared spectrometry, scanning electron microscopy, energy-dispersive X-ray spectroscopy and X-ray photoelectron spectroscopy. It was found that the grafting amount of 4-styrenesulfonic acid increased linearly with the polymerization time. Effect of parameters such as pH, initial concentration and temperature on the removal of heavy metal ions was studied. The maximum adsorption capacities of the resin were up to 275 mg·g –1 for Pb(II), 169 mg·g –1 for Cu(II), 115 mg·g –1 for Cd(II), 90.8 mg·g –1 for Cr(III) and 75 mg·g –1 for Ni(II) at pH 5.0. The adsorption isotherms of the five metal ions were best described by the Langmuir model, and their adsorption kinetics followed the pseudo-second-order kinetic equation. Thermodynamic studies showed that the adsorption was spontaneous. Furthermore, the resins still maintained good adsorption capacity after 10 cycles of adsorption–regeneration. All the results suggested that the resin could be potentially applied to the efficient removal of heavy metal ions from wastewater.
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Advanced Science
·
2025
·
IF 14.1
Renjie Cao,Tingting Zhao,Ying Wang,Shaofei Song,Yuan Li,Mengling Hou,Yanxin Zhang,Sengpeng Wong,Siqi Wang,Yuran Wang,Hong Peng,Min Huang,Yiming Jiang
Macrophage proinflammatory hyperactivation drives the pathogenesis of acute liver injury, a common complication of sepsis. The role of the nuclear receptor constitutive androstane receptor (CAR) in endotoxin-induced liver injury remains unclear. Here, this study reports that CAR is highly expressed in human and murine macrophages. CAR activation markedly attenuated endotoxin-induced liver damage, alleviating hepatocyte death and hepatic inflammation. Macrophage-hepatocyte coculture confirmed that CAR inhibited inflammation through macrophage crosstalk. CAR-mediated hepatoprotection and anti-inflammatory effects are absent in AAV8- F4/80- sh Car -treated mice, confirming the essential role of CAR in macrophages. Mechanistically, CAR is found to interact with Tlr4 , and the suppressive effects of CAR on TLR4 are proven in Tlr4 −/− mice. Furthermore, CAR activation reduced LPS-induced inflammation in hMDMs, BMDMs, RAW264.7, and THP-1 cells, and Car or Tlr4 knockdown abolished CAR-mediated immunosuppression. Overall, these findings showed that macrophage CAR activation attenuated endotoxin-induced liver injury and hepatic inflammation through the TLR4 signaling pathway, providing insights for treating inflammatory liver diseases.
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MOLECULES
·
2025
·Vol. 30
·期 16
·3400
·
IF 4.6
Lina Hu,Mengjiao Li,Zheng Liu,Hui Yan,Xuekun Wang,Shiben Wang
To discover novel, potent anticonvulsant compounds, a series of compounds containing triazole structural fragments were synthesized and evaluated for their anticonvulsant activity. Compounds6f(maximal electroshock (MES), 50% effective dose (ED50) = 13.1 mg/kg; subcutaneous pentylenetetrazole (scPTZ), ED50= 19.7 mg/kg) and6l(MES, ED50= 9.1 mg/kg;scPTZ, ED50= 19.0 mg/kg) showed the best activity in MES andscPTZ tests. The results of an affinity test showed that the potent compounds had better binding to the GABAAreceptors. The results of an inhibitory neurotransmitter GABA content test in brain tissue indicated that compounds6fand6lexerted anticonvulsant activity which may be associated with the increase of GABA content in the rat brain. Elevated plus maze (EPM) assay results showed that compounds6fand6lpossessed anxiolytic effects, and their effects correlated with the binding sites of benzodiazepines (BZs) in the GABAAreceptors. Molecular docking was performed to investigate the interactions of the studied compound6lwith the GABAAreceptors on the molecular level.
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JOURNAL OF APPLIED POLYMER SCIENCE
·
2025
·
IF 2.8
Yukun Hu,Min Wang,Longyu Tian,Guangming Liao,Zaijun Lu
The novel cross-linked copolymer (BPSU-C4A-SPS) was synthesized by the copolymerization of benzoxazine-functionalized polysulfone (BPSU), calix[4]arene (C4A), and sodium 4-hydroxybenzenesulfonate (SPS). The material exhibited high thermal stability with a glass transition temperature ( T g ) of 285°C and a 5% weight loss temperature ( T d5 ) of 347°C, and enhanced hydrophilicity with a water contact angle of 76.5°. Moreover, adsorption studies on MB demonstrated that BPSU-C4A-SPS possesses a higher adsorption capacity than the benzoxazine-functionalized polysulfone homopolymer (BPSU-a) and the benzoxazine-functionalized polysulfone-calix[4]arene copolymer (BPSU-C4A). Adsorption followed the Freundlich isotherm and pseudo-second-order kinetics best describes the adsorption process of BPSU-C4A-SPS to MB, indicating multilayer chemisorption driven by electrostatic interactions and π-π stacking. This work provides a scalable strategy for designing stable adsorbents with synergistic effects, suitable for industrial wastewater treatment.
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MEDICINAL CHEMISTRY RESEARCH
·
2019
·Vol. 28
·期 9
·1388-1401
·
IF 1.72
Chen Xin,Liu Xiujie,Qiu kai,Xu Xiang,Li Caiwen,Wang Yan
According to the bio-isosterism theory, a series of N , N ’-disubstitutedphenyl-4-ethoxylbenzene-1, 3-disulfonamides ( 5a - p ) were designed and synthesized by two steps of reactions including chlorosulfonation and ammonolysis. The structures of all compounds have been confirmed by IR, 1 H-NMR, 13 C-NMR, and ESI-MS spectra. The in vitro anti-platelet aggregation activities were evaluated by Born’s test induced by adenosine diphosphate (ADP) and arachidonic acid (AA), respectively. The biological evaluation results revealed that compound 5h had the lowest IC 50 value (0.32 μM) and the highest inhibition rate (40.9 %) that of three positive control agents clopidogrel (0.41 μM, 23.5 %), aspirin (0.53 μM, 28.9 %), and picotamide (0.76 μM, 32.7 %). Afterwards, compounds with higher activities were selected to further study in vitro cytotoxicity via cell counting kit-8 (CCK-8) assay. The cytotoxicity results indicated that compound 5h had simultaneously the lowest cytotoxicity, while other compounds had no significant relationship between the anti-platelet activities and cytotoxicities. Based on above in vitro anti-platelet activity data, the SAR (Structure Activity Relationship) of the target compounds was preliminarily summarized. In general, N , N ’-disubstitutedphenyl-4-ethoxylbenzene-1, 3-disulfonamides have the potential of further study and very likely become safer and more effective anti-platelet agents.
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MOLECULES
·
2022
·Vol. 27
·期 12
·3738
·
IF 4.927
Xia Niu,Yanan Meng,Yucheng Wang,Guiling Li
As a global health problem, liver fibrosis still does not have approved treatment. It was proved thatN-(3,4,5-trichlorophenyl)-2(3-nitrobenzenesulfonamide) benzamide (IMB16-4) has anti-hepatic fibrosis activity. However, IMB16-4 displays poor water solubility and poor bioavailability. We are devoted to developing biodegraded liposome-coated polymeric nanoparticles (LNPs) as IMB16-4 delivery systems for improving aqueous solubility, cellular uptake, and anti-fibrotic effects. The physical states of IMB16-4−LNPs were analyzed using a transmission electron microscope (TEM), high-performance liquid chromatography (HPLC), Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD) and differential scanning calorimeter (DSC). The results show that IMB16-4−LNPs increased the drug loading compared to liposomes and enhanced cellular uptake behavior compared with IMB16-4−NPs. In addition, IMB16-4−LNPs could repress the expression of hepatic fibrogenesis-associated proteins, indicating that IMB16-4−LNPs exhibited evident anti-fibrotic effects.Keywords:liposome-coated polymeric nanoparticles;IMB16-4;liver fibrosis;hepatic stellate cells;cell uptake
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NEW JOURNAL OF CHEMISTRY
·
2022
·Vol. 46
·期 5
·2335-2350
·
IF 3.925
Wen-Yan Wang,Zi-Hui Yang,A-Liang Li,Qing-Song Liu,Yue Sun,Wen Gu
A series of novel 2-amino-4-aryl-pyrimidine derivatives of ursolic acid were designed, synthesized, and evaluated for their anticancer activities against four cancer cell lines (MCF-7, HeLa, HepG2, and A549) and a human hepatocyte cell line (LO2) via MTT assay. Among these derivatives, compound 7b exhibited potent cytotoxic activity against MCF-7 and HeLa cells with IC50 values of 0.48 ± 0.11 and 0.74 ± 0.13 μM, respectively, and substantially lower cytotoxicity to LO2 cells. Further cellular mechanism studies in MCF-7 cells elucidated that compound 7b could inhibit cell migration, induce cell cycle arrest at S phase and trigger mitochondrial-related apoptosis by increasing the generation of intracellular ROS and decreasing the mitochondrial membrane potential (MMP), which was associated with upregulation of the protein expression level of Bax and downregulation the level of Bcl-2 and the activation of caspase cascade. Western blot analyses also revealed that compound 7b could simultaneously suppress RAS/Raf/MEK/ERK and PI3K/AKT/mTOR signaling pathways, which could be responsible for the induction of apoptosis. Molecular docking study revealed that MEK1 kinase could be one of the possible targets of the title compounds. These results offered a promising scaffold for the investigation of novel targeted anticancer agents.
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Biomed Research International
·
2022
·
IF 3.246
Cheng Shi,Fei Yuan,Zhilong Li,Zhenhua Zheng,Changliang Yuan,Ziyang Huang,Jianping Liu,Xuping Lin,Taoyi Cai,Guofeng Huang,Zhenqi Ding
Background. The microenvironment of bone defects displayed that M2 polarization of macrophagocyte could promote the osteoblast growth and benefit the wound healing. Bone scaffold transplantation is considered to be one of the most promising methods for repairing bone defects. The present research was aimed at constructing a kind of novel bone scaffold nanomaterial of [email protected] for treating bone defects responding to the wound microenvironment of bone defects and elucidating the mechanics of [email protected] treating bone defect via controlling release of IL-4, inducing M2 polarization and active factor release of macrophagocyte, and eventually relieving osteoblast injury. Methods. [email protected] was firstly fabricated and its release of IL-4 was assessed in vitro. Following, the effects of [email protected] nanocomplex on the release of active factors of macrophage were examined using Elisa assay and promoting M2 polarization of the macrophage by immunofluorescence staining. And then, the effects of active factors from macrophage supernatant induced by [email protected] on osteoblast growth were examined by CCK-8, flow cytometry, and western blot assay. Results. The release curve of IL-4 in vitro displayed that there was more than 80% release ratio for 30th day with a sustained manner in pH 5.5. Elisa assay data showed that [email protected] nanocomplex could constantly promote the release of proproliferative cytokine IL-10, SDF-1α, and BMP-2 in macrophagocyte compared to only IL-4 treatment, and immunofluorescent image showed that [email protected] could promote M2 polarization of macrophagocytes via inducing CD206 expression and suppressing CD86 expression. Osteoblast injury data showed that the supernatant from macrophagocyte treated by [email protected] could promote the osteoblast proliferation by MTT assay. Flow cytometry data showed that the supernatant from macrophagocyte treated by [email protected] could suppress the osteoblast apoptosis from 22.1% to 14.6%, and apoptosis-related protein expression data showed that the supernatant from macrophagocyte treated by [email protected] could suppress the expression of Bax, cleaved caspase 3, and cleaved caspase 8. Furthermore, the immunofluorescent image showed that the supernatant from macrophagocyte treated by [email protected] could inhibit nucleus location of p65, and western blot data showed that the supernatant from macrophagocyte treated by [email protected] could suppress the phosphorylation of IKK and induce the expression of IκB. Conclusion. [email protected] could control the sustaining release of IL-4, and it exerts the protective effect on osteoblast injury via inducing M2 polarization and proproliferative cytokine of macrophagocyte and following inhibiting the apoptosis and NF-κB pathway-associated inflammation of osteoblast.
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