Journal of Biomedical Nanotechnology
·
2022
·Vol. 18
·期 11
·2484-2497
·
IF 3.641
Li Guiyin,Chen Min,Li Haimei,Li Xinhao,Yao Xiaoqing,Li Liuxun
Golgi protein 73 (GP73) is a novel biomarker for the detection of hepatocellular carcinoma. We prepare a colorimetric aptasensor for GP73 detection based on the catalytic performance of the H-rGO-Mn3O4 nanozyme. The H-rGO-Mn3O4 nanozyme serves
as a peroxidase mimetic which can enhance the reaction with the substrate (3, 3,’ 5, 5′-tetramethylbenzidine, TMB) and H2O2. The peroxidase-like activity of H-rGO-Mn3O4 features a 1.78-fold higher binding affinity value (Km
)
for TMB compared with that of horseradish peroxidase. The H-rGO-Mn3O4 nanozyme not only exhibits admirable peroxidase-like activities due to the synergistic effect of Mn3O4 NPs, rGO and hemin, but also has a large specific surface area to endow the
GP73 aptamer with specific recognition capabilities. A sandwich colorimetric aptasensor is formed to realize the visual detection of GP73 through catalyzing the H2O2-mediated oxidation peroxidase substrate TMB to oxidized TMB accompanied by a color change from colorless
to blue. Under optimal conditions, the logarithm of the GP73 concentration (0.05–50.0 ng/mL) shows a good linear relationship with the absorbance. The calibration equation is Y =−0.0383 lgC+0.4835, with R2 of 0.9964, and the lowest limit of detection is 36.94
pg/mL. Additionally, the content of GP73 in human serum samples is directly detected, and the relative standard deviation is 0.49–4.91%. Compared with the enzyme-linked immunosorbent assay method, the relative error is 0.23–3.61%. All in all, colorimetric aptasensor is demonstrated
to exhibit excellent specificity, stability, and reproducibility.
研究领域:
生物标志物研究
·
纳米技术
·
分析化学
·
癌症诊断
·
生物化学
✓ 已验证
DOI
高影响因子
CHEMOSPHERE
·
2020
·
IF 5.778
Fengxia Chen,Daojian Tang,Ya Wang,Tuo Li,Jiahai Ma
A novel catalyst which integrates heterogeneous and homogenous Fenton reactions is designed and fabricated by encapsulating 2,5-dihydroxy-1,4-benzoquinone (2,5-DBQ) in ECDP-Fe 3 O 4 , a composite of Fe 3 O 4 nanoparticles immobilized on a β-cyclodextrin polymer (ECDP) with ethylene diamine tetraacetic acid (EDTA) as cross-linking agent. The 2,5-DBQ@ECDP-Fe 3 O 4 has superior catalytic performance for 4-nitrophenol and 2,4-dichlorophenol degradation compared with control systems. Mechanism study revealed that although the initial active site is Fe 3 O 4 loaded on ECDP, the actually catalyst is the iron ions released from Fe 3 O 4 but confined within the composite. EDTA in β-cyclodextrin polymer can improve both the solubility and adsorption capacity to H 2 O 2 of Fe 3 O 4 . The quinone molecules 2,5-DBQ in the β-cyclodextrin cavity can accelerate Fe 3+ /Fe 2+ cycle adjacent to the cavity, thus in favor of the decomposition of H 2 O 2 into OH as main reactive oxidizing species. The current catalyst integrates the advantages of homogeneous and heterogeneous advanced oxidation processes and is promising in practical applications.
研究领域:
环境科学
·
催化
·
化学工程
·
材料科学
✓ 已验证
DOI
高影响因子
Marine Drugs
·
2024
·Vol. 22
·期 9
·419
·
IF 4.9
Yun-Han Zhang,Ya-Ting Zou,Yong-Yi Zeng,Lan Liu,Bi-Shuang Chen
This study explores the reasons behind the variations in the enantioselectivity of the sulfoxidation of methyl phenyl sulfide by marine-derived vanadium-dependent haloperoxidases (VHPOs). Twelve new VHPOs of marine organisms were overexpressed, purified, and tested for their ability to oxidize sulfide. Most of these marine enzymes exhibited nonenantioselective behavior, underscoring the uniqueness ofAnVBPO from the brown seaweedAscophyllum nodosumandCpVBPO from the red seaweedCorallina pilulifera, which produce (R)- and (S)-sulfoxides, respectively. The enantioselective sulfoxidation pathway is likely due to direct oxygen transfer within the VHPO active site. This was demonstrated through molecular docking and molecular dynamics simulations, which revealed differences in the positioning of sulfide withinAnVBPO andCpVBPO, thus explaining their distinct enantioselectivities. Nonenantioselective VHPOs probably follow a different oxidation pathway, initiating with sulfide oxidation to form a positively charged radical. Further insights were gained from studying the catalytic effect of VO43−on H2O2-driven sulfoxidation. This research improves the understanding of VHPO-mediated sulfoxidation and aids in developing biocatalysts for sulfoxide synthesis.
研究领域:
生物化学
·
海洋生物学
·
酶学
·
分子生物学
✓ 已验证
DOI
PubMed
高影响因子
CHEMOSPHERE
·
2018
·
IF 4.427
Zhuangpeng Huang,Zhiping Chen,Yuancai Chen,Yongyou Hu
Iron-copper bimetal supported on mesoporous γ-Al 2 O 3 with different Fe/Cu ratios was prepared via a modified evaporation-induced self-assembly method and employed to degrade 4-chlorophenol (4-CP) in a Fenton-like process. The X-ray photoelectron spectroscopy, X-ray diffraction spectrum and UV–vis diffuse reflectance spectroscopy results confirmed the co-doping of Fe 2+ /Fe 3+ and Cu + /Cu 2+ into the lattice of γ-Al 2 O 3 through the bonds of Al O Fe and Al O Cu. With the iron and copper co-doped in γ-Al 2 O 3 , three-fold oxygen vacancies (V 0 ) could be achieved compared with that of non-metal catalysts, which was favorable for the reduction of iron and copper species. The 1Fe3Cu-γ-Al 2 O 3 with a Fe/Cu mass ratio of 1:3 exhibited the highest activity on Fenton-like degradation of 4-CP due to the synergistic effects of Fe and Cu by facilitating the electron transfer in the recycles of Cu + /Cu 2+ and Fe 2+ /Fe 3+ based on the results of X-ray photoelectron spectroscopy, cyclic voltammetry and electro paramagnetic resonance analysis. The utilization efficiency of H 2 O 2 was as high as 86.0% when 4-CP almost disappeared (i.e., 99.7 percent of 0.78 mM 4-CP) at 240 min and pH 7.0, additionally, their dechlorination and TOC removal efficiencies were 92.0% and 81.6%, respectively. The catalyst also displayed a good performance after 5th cycles. Furthermore, a possible mechanism for activation of OH on the catalyst surface was also proposed.
研究领域:
环境化学
·
催化
·
水处理
✓ 已验证
DOI
高影响因子
LANGMUIR
·
2025
·
IF 3.9
Haixia Li,Chuanqiang Yin,Dan Li,Feigao Xu
In this study, oxygen vacancy-engineered FeP/BiOBr heterojunction composites (FB-3) were synthesized via a facile hydrothermal method to achieve enhanced tetracycline hydrochloride (TCH) degradation through a visible-light-driven photo-Fenton process. Systematic characterizations (SEM, XRD, XPS, and EPR) confirmed the successful integration of FeP with BiOBr, which modulated the band structure, introduced abundant oxygen vacancies (OVs), and suppressed photogenerated carrier recombination. Under the optimized conditions (0.2 g L–1 catalyst, 10 mM H2O2, and pH 4.2), the FB-3 composite achieved 94.2% TCH removal at an initial concentration of 40 mg L–1 within 90 min, with 73.9% TOC mineralization and minimal iron leaching of only 0.21 mg L–1. Mechanistic studies revealed that OVs acted as electron traps, accelerating Fe2+/Fe3+ redox cycling and synergistically activating H2O2 to generate dominant reactive radicals (•OH and •O2–). Radical trapping experiments and EPR analysis validated the critical roles of photogenerated carriers and OVs in enhancing surface kinetics. The composite exhibited robust performance in diverse water matrices, demonstrating potential for antibiotic wastewater treatment.
研究领域:
环境科学
·
催化
·
材料科学
·
化学工程
·
水处理技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Advanced Healthcare Materials
·
2024
·
IF 10
Jiaxin Dong,Guanxiong Liu,Yuri V. Petrov,Yujie Feng,Dechang Jia,Vladimir E Baulin,Aslan Yu Tsivadze,Yu Zhou,Baoqiang Li
Iron phosphide/carbon (FeP/C) serving as electrocatalysts exhibit excellent activity in oxygen reduction reaction (ORR) process. H 2 O 2 catalyzed by peroxidase (POD) is similar to the formation of new electron transfer channels and the optimization of adsorption of oxygen-containing intermediates or desorption of products in ORR process. However, it is still a challenge to discover FeP/C with enhanced POD-like catalytic activity in the electrocatalytic database for biocatalysis. The discovery of FeP/carbon dots (FeP/CDs) nanozymes driven by electrocatalytic activity for enhanced POD-like ability is demonstrated. FeP/CDs derived from CDs-Fe 3+ chelates show enhanced POD-like catalytic and antibacterial activity. FeP/CDs exhibit enhanced POD-like activities with a specific activity of 31.1 U mg −1 that is double higher than that of FeP. The antibacterial ability of FeP/CDs nanozymes with enhanced POD-like activity is 98.1%. The antibacterial rate of FeP/CDs nanozymes (250 µg mL −1 ) increased by 5%, 15%, and 36% compared with FeP, Fe 2 O 3 /CDs, and Cu 3 P/CDs nanozymes, respectively. FeP/CDs nanozymes will attract more efforts to discover or screen transition metal phosphide/C nanozymes with enhanced POD-like catalytic activity for biocatalysis in the electrocatalytic database.
研究领域:
材料科学
·
纳米技术
·
生物化学
·
催化
·
生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Nature Communications
·
2024
·Vol. 15
·期 1
·1-16
·
IF 14.7
Si Haibin,Du Dexin,Jiao Chengcheng,Sun Yan,Li Lu,Tang Bo
In enzymatic catalysis, the redox site and Lewis acid are the two main roles played by metal to assist amino acids. However, the reported enzyme mimics only focus on the redox-active metal as redox site, while the redox-inert metal as Lewis acid has, to the best of our knowledge, not been studied, presenting a bottleneck of enzyme mimics construction. Based on this, a series of highly efficient M x V 2 O 5 ·nH 2 O peroxidase mimics with vanadium as redox site and alkaline-earth metal ion (M 2+ ) as Lewis acid are reported. Experimental results and theoretical calculations indicate the peroxidase-mimicking activity of M x V 2 O 5 ·nH 2 O show a periodic change with the Lewis acidity (ion potential) of M 2+ , revealing the mechanism of redox-inert M 2+ regulating electron transfer of V-O through non-covalent polarization and thus promoting H 2 O 2 adsorbate dissociation. The biomimetic synergetic effect of redox site and Lewis acid is expected to provide an inspiration for design of enzyme mimics.
研究领域:
生物化学
·
催化
·
材料科学
·
酶学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Minggui Gong,Jiajie Su,Ying Chen,Kaixin Zheng,Lijie Yuan,Fangyuan Qin,Huawei Niu,Yong Ye
Heavy metal ions tend to accumulate through the food chain and may cause many serious diseases. As an antioxidant, cysteine (Cys) plays a crucial role in regulating redox homeostasis. In addition, Cys is also widely used in the food industry. Therefore, the development of a convenient, sensitive, and practical tool for detecting Cys is of great significance for investigating the physiological and pathological functions of Cys induced by heavy metal ions. In this paper, a fluorescent probe NYVB with aggregation-induced emission (AIE) activities has been designed and synthesized, which featured good advantages in Cys detection, including favorable selectivity, desirable sensitivity, wide linear range, and low detection limit. The prepared solid-state sensor enabled the ratiometric visual detection of Cys. Probe NYVB has been successfully implemented for testing Cys in real food samples and serum samples with satisfactory recoveries. In addition, probe NYVB has been successfully employed for detecting endogenous and exogenous Cys in SKOV3 cells. More excitingly, probe NYVB has also been used to track the detection of Cys in H 2 O 2 , Hg 2+ , or Cu 2+ -induced redox imbalance. Overall, probe NYVB will open up a new avenue for studying Cys in the amelioration of heavy metal toxicity and has practical applications.
研究领域:
分析化学
·
生物化学
·
毒理学
·
食品科学
·
细胞生物学
✓ 已验证
产品关联
DOI
高影响因子
ACS Biomaterials Science & Engineering
·
2024
·
IF 5.4
Beibei Zhang,Hao Liu,Yifei Wang,Yong Zhang
Ferroptosis is an appealing cancer therapy strategy based on the H2O2-involved Fenton reaction to produce toxic •OH for lipid peroxidation. However, intracellular H2O2 is easily consumed and results in a deficient Fenton reaction. This obstacle can be overcome by traditional chemotherapeutic drugs for H2O2 supplements. Moreover, a recent work illustrated that dihydroartemisinin (DHA) could promote ferroptosis against tumoral cells, particularly in the presence of ferrous compounds. To achieve combined chemotherapy and ferroptosis, a nanocarrier (TKNPDHA-Fc) was constructed by using thioketal (TK)-bridged paclitaxel prodrug (PEG-TK-PTX) and ferrocene (Fc)-conjugated PEG-Fc, where DHA was encapsulated by a hydrophobic–hydrophobic interaction. Upon cellular uptake, TKNPDHA-Fc could facilitate PTX release through TK breakage under an excess H2O2 microenvironment. Owing to the loss of the hydrophobic PTX component, TKNPDHA-Fc underwent a rapid dissociation for improving DHA to act as a ferroptotic inducer along with Fe supplied from Fc. Moreover, both the chemotherapy-induced reactive oxygen species and the •OH produced from reinforced ferroptosis further stimulated the TK cleavage. The “self-catalytic” loop of TKNPDHA-Fc remarkably improved the antitumor performance in vivo via combined mechanisms, and its tumor inhibition rate reached 78.3%. This work highlights the contribution of ROS-responsive and self-catalytic nanoplatforms for enhancing the potential of combined chemotherapy and ferroptosis for cancer therapy in the future.
研究领域:
癌症治疗
·
纳米技术
·
药学
·
生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Small
·
2024
·
IF 13
Rongshuo Guo,Linghua Jin,Ye Zhang
Piezo-catalysis emerges as an efficient, safe, and affordable strategy for removing hazardous substances from aquatic environments. Here, the BiFeO 3 @In 2 Se 3 heterojunction demonstrates remarkable prowess as a piezo-catalyst, enabling the high-efficiency removal of uranium (U) from U(VI)-containing water. A total U(VI) removal efficiency of 94.6% can be achieved under ultrasonic vibration without any sacrificial agents. During the entire catalytic process, piezo-induced electrons, hydroxyl radicals, and superoxide radicals play important roles in U(VI) removal, while the generated H 2 O 2 is responsive to the transformation of soluble U(VI) into insoluble (UO 2 )O 2 •2H 2 O and UO 3 . Furthermore, auxiliary illumination can accelerate the increase of free charges, enabling the piezo-catalyst to retain more charges. This leads to an improved U(VI) removal efficiency of 98.8% and a significantly increased reaction rate constant. This study offers a comprehensive analysis of the fabrication of high-efficiency piezo-catalysts in the removal or extraction of U(VI) from U(VI)-containing water.
研究领域:
环境科学
·
材料科学
·
核工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Materials Today Chemistry
·
2024
·
IF 6.7
Xiuwen Gao,Woding Deng,Jinhui Tan,Xiong Shuai,Jun Zan,Tailai Ye,Kaiwu Luo,Fangwei Qi,Yujun Wei,Cijun Shuai
Thermoelectric material could realize the thermal-electricity-reactive oxygen species (ROS) conversion under temperature gradient, being considered a promising candidate for tumor therapy. Nevertheless, it required exogenous excitation such as light irradiation, causing difficultity in achieving on-demand therapy. Herein, a tumor microenvironment responsive Bi 2 Te 3 –CaO 2 nanohybrid was synthetized by in situ growing calcium peroxide (CaO 2 ) nanoparticles on thermoelectric bismuth telluride (Bi 2 Te 3 ) nanosheets, and then blended with poly- l -lactic acid (PLLA) to fabricate a PLLA/Bi 2 Te 3 –CaO 2 scaffold. Once the acidic microenvironment of the tumor was sensed, the CaO 2 would serve as an intelligent switch, releasing heat through rapid hydrolysis and triggering the thermoelectrocatalysis of Bi 2 Te 3 to generate ROS, thereby achieving on-demand therapy. Results demonstrated that the scaffold exhibited rapid temperature rise and significant ROS catalytic formation ability in an acidic solution (pH 5.5), while no significant changes occurred in a neutral environment (pH 7.4). Annexin V-FITC/PI staining and JC-1 assay demonstrated that the generated ROS could induce tumor apoptosis by reducing mitochondrial membrane potential. Besides, the fluorescent probe test revealed that CaO 2 could also produce hydrogen peroxide (H 2 O 2 ) and calcium ions (Ca 2+ ) to interfere with intracellular Ca 2+ , thereby further enhancing the antitumor effect. Overall, this work proposed a novel perspective for on-demand antitumor therapy.
研究领域:
生物医学工程
·
材料科学
·
肿瘤学
·
纳米技术
✓ 已验证
产品关联
DOI
高影响因子
PLoS One
·
2024
·Vol. 19
·期 5
·e0301358
·
IF 2.9
Chuang Wei,Yijun Gao,Peifeng Li
Drug-resistant bacteria arising from antibiotic abuse infections have always been a serious threat to human health. Killing bacteria with toxic reactive oxygen species (ROS) is an ideal antibacterial method for treating drug-resistant bacterial infections. Here, we prepared Pt-Ru bimetallic nanoclusters (Pt-Ru NCs) with higher peroxidase (POD)-like activity than Pt monometallic nanoclusters. Pt-Ru can easily catalyze the decomposition of H 2 O 2 to produce ·OH, thereby catalyzing the transformation of 3,3’,5,5’-tetramethylbiphenylamine (TMB) to blue oxidized TMB (oxTMB). We utilized the POD-like activity of the Pt-Ru NCs for antibacterial therapy. The results showed that at doses of 40 μg/mL and 16 μg/mL, the Pt-Ru NCs exhibited extraordinary antibacterial activity against E . coli and S . aureus , demonstrating the enormous potential of Pt-Ru NCs as antibacterial agents.
研究领域:
纳米技术
·
生物材料
·
抗菌研究
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）