Tissue Engineering and Regenerative Medicine
·
2023
·
IF 3.6
Ye Yuanjian,Zhong Haobo,Huang Shoubin,Lai Weiqiang,Huang Yizhi,Sun Chunhan,Zhang Yanling,Zheng Shaowei
Background: Implantation of bone marrow mesenchymal stem cells (BMSCs) is a potential alternative for promoting bone defects healing or osseointegration in osteoporosis. However, the reactive oxygen species (ROS) accumulated and excessive inflammation in the osteoporotic microenvironment could weaken the self-replication and multi-directional differentiation of transplanted BMSCs. Methods: In this study, to improve the hostile microenvironment in osteoporosis, Poloxamer 407 and hyaluronic acid (HA) was crosslinked to synthetize a thermos-responsive and injectable hydrogel to load MnO 2 nanoparticles as a protective carrier (MnO 2 @Pol/HA hydrogel) for delivering BMSCs. Results: The resulting MnO 2 @Pol/HA hydrogel processed excellent biocompatibility and durable retention time, and can eliminate accumulated ROS effectively, thereby protecting BMSCs from ROS-mediated inhibition of cell viability, including survival, proliferation, and osteogenic differentiation. In osteoporotic bone defects, implanting of this BMSCs incorporated MnO 2 @Pol/HA hydrogel significantly eliminated ROS level in bone marrow and bone tissue, induced macrophages polarization from M1 to M2 phenotype, decreased the expression of pro-inflammatory cytokines (e.g . , TNF-α, IL-1β, and IL-6) and osteogenic related factors (e.g., TGF-β and PDGF). Conclusion: This hydrogel-based BMSCs protected delivery strategy indicated better bone repair effect than BMSCs delivering or MnO 2 @Pol/HA hydrogel implantation singly, which providing a potential alternative strategy for enhancing osteoporotic bone defects healing.
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Journal of Infrared Millimeter and Terahertz Waves
·
2023
·Vol. 44
·期 9
·780-794
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IF 2.9
Guan Hanxiao,Xie Chen,Cao Yuqi,Huang Pingjie,Hou Dibo,Zhang Guangxin
In this paper, we propose a discrimination model for abnormal sialic acid content using terahertz time and frequency-domain features of samples in an attempt to explore the significance of human serum sialic acid content in early cancer screening. Specifically, the time-domain spectral features were obtained by applying variational mode decomposition (VMD) for time-domain spectrum preprocessing and composite multi-scale entropy (CMSE) for feature extraction. The frequency-domain spectral features were acquired using principal component analysis (PCA). The model was then constructed on the basis of support vector machine (SVM). To verify the reliability of this discrimination model, we used three different concentrations of albumin solutions to simulate the serum environment in the human body and mixed them with sialic acid solutions of eight different concentrations. The results indicate that our discrimination model achieved an accuracy of 91.01% for the detection of abnormal sialic acid content. This study provides a foundation for the accurate quantitative detection of biomarkers in human serum.
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Scientific Reports
·
2023
·Vol. 13
·期 1
·1-14
·
IF 4.6
Alishiri Mahsa,Gonbadi Maryam,Narimani Mehdi,Abdollahi Seyyed Amirreza,Shahsavaripour Negin
The contamination of the aquatic environment with antibiotics is among the major and developing problems worldwide. The present study investigates the potential of adsorbent magnetite-chitosan nanoparticles (Fe 3 O 4 /CS NPs) for removing trimethoprim (TMP) and sulfamethoxazole (SMX). For this purpose, Fe 3 O 4 /CS NPs were synthesized by the co-precipitation method, and the adsorbent characteristics were investigated using XRD, SEM, TEM, pH zpc , FTIR, and VSM. The effect of independent variables (pH, sonication time, adsorbent amount, and analyte concentration) on removal performance was modeled and evaluated by Box–Behnken design (BBD). The SEM image of the Fe 3 O 4 /CS adsorbent showed that the adsorbent had a rough and irregular surface. The size of Fe 3 O 4 /CS crystals was about 70 nm. XRD analysis confirmed the purity and absence of impurities in the adsorbent. TEM image analysis showed that the adsorbent had a porous structure, and the particle size was in the range of nanometers. In VSM, the saturation magnetization of Fe 3 O 4 /CS adsorbent was 25 emu g −1 and the magnet could easily separate the adsorbent from the solution. The results revealed that the optimum condition was achieved at a concentration of 22 mg L −1 , a sonication time of 15 min, an adsorbent amount of 0.13 g/100 mL, and a pH of 6. Among different solvents (i.e., ethanol, acetone, nitric acid, and acetonitrile), significant desorption of TMP and SMX was achieved using ethanol. Also, results confirmed that Fe 3 O 4 /CS NPs can be used for up to six adsorption/desorption cycles. In addition, applying the Fe 3 O 4 /CS NPs on real water samples revealed that Fe 3 O 4 /CS NPs could remove TMP and SMX in the 91.23–95.95% range with RSD (n = 3) < 4. Overall, the Fe 3 O 4 /CS NPs exhibit great potential for removing TMP and SMX antibiotics from real water samples.
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POLYMER BULLETIN
·
2023
·
IF 3.2
Liu Yuyao,Yao Ruixiang,Tong Yujie,Lu Yaqing,Guo Qiyang
Nanoparticles (NPs), especially inorganic NPs, have been used as fillers to improve the insulation properties of polymers. However, inorganic NPs is difficult to disperse well in polymer matrix, which inhibit the development of insulating nanocomposites for high-voltage direct current power cable. Therefore, it is necessary to improve the dispersion of NPs in the polymer matrix in order to make NPs play a greater role. In this work, polystyrene NPs (PS-NPs) were synthesized and used as fillers to improve the insulation properties of LLDPE. SEM images show that PS-NPs are monodispersed in LLDPE matrix without thermal fusion when the content of PS-NPs is less than 1 wt%. The results of DSC illustrate that the addition of PS-NPs had no effected on thermal behavior of LLDPE. With the introduction of PS-NPs, the DC breakdown strength of LLDPE/PS-NPs is first increased and then decreased. The highest value of 346.5 kV/mm is obtained by PS-0.1 with 0.1 wt% of PS-NPs. The electric conductivity of all LLDPE/PS-NPs composites are decreased comparing with neat LLDPE. Meanwhile, PS-NPs had no significant influence on the dielectric permittivity and dielectric loss of the complex. Therefore, PS-NPs is an alternative option to improve the insulating performance, and our results provide a way for the development of nanocomposites insulating material.
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MICROCHIMICA ACTA
·
2023
·Vol. 191
·期 1
·1-11
·
IF 5.7
Huang Lianrun,Wang Guixiu,Wu Yanling,Wang Zhijia,Ding Yuan,Liang Hongwu,Hua Xiude
Peptidomimetic and anti-immunocomplex peptides can be easily isolated from phage display libraries, and can be used as alternatives to chemical competing haptens to improve the sensitivity of small molecule immunoassay. In this work, 16 peptidomimetics and 7 anti-immunocomplex peptides of pendimethalin (PND) were obtained from cyclic 7-, 8-, 9-, and 10-residue peptide phage libraries. Peptidomimetic EJ-2 (CMFTGTDFPC) with the highest sensitivity in competitive phage enzyme-linked immunosorbent assay (ELISA) and immunocomplex peptide EF-30 (CNPGWPPIPC) with the highest sensitivity in noncompetitive phage ELISA were selected to prepare phage-free peptides with GGGSSK-biotin at the C-terminus. Competitive and noncompetitive lateral flow immunoassays (CLFIA and NLFIA) were developed by using the phage-free peptides. After optimization, the CLFIA and NLFIA showed visual limit of detections (vLODs) of 5 ng/mL and 2.5 ng/mL, respectively, which were improved two- and fourfold compared with a LFIA based on chemical hapten. The NLFIA showed better sensitivity than CLFIA in the detection of spiked samples, and can meet the detection requirements for agro-products regulated by EU and China. The detection results of CLFIA and NLFIA for blind samples were consistent with that of ultra performance liquid chromatography/tandem mass spectrometry. Graphical abstract
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CELLULOSE
·
2023
·Vol. 30
·期 16
·10285-10296
·
IF 5.7
Gao Yan,Zhang Yuangong,Wang Yu,Liu Bing,Liu Feng,Ba Xinwu
In this paper, a cellulose-based hydrogel with the ability to lower glucose levels, scavenge reactive oxygen species (ROS), and colorimetrically monitor early lesions was synthesized to address the issue of glucose and H 2 O 2 overexpression on the wound surface. Briefly, boronic acid pinacol ester containing phenolphthalein (BAPE-PPH) was employed as a crosslinker to react with hydroxypropyl methylcellulose (HPMC) to afford hydrogels (HPMC@PPH-Hs) with good biocompatibility. Because glucose has higher affinity for the phenylboronic ester group than cellulose derivatives, the HPMC@PPH-Hs showed excellent glucose-lowering performance. The presence of B-C bonds in the network endows the as-obtained hydrogel with H 2 O 2 sensitivity, and cleavage of the B-C bonds forces the transformation of BAPE-PPH moieties into PPH, giving rise to colorimetric behaviour. Thus, the obtained HPMC@PPH-Hs provide information to enrich the family of smart hydrogels, and its potential application in diabetic wound healing may be desirable. Graphical abstract
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Cell Death Discovery
·
2023
·Vol. 9
·期 1
·1-10
·
IF 7
Wang Xianzhe,Shi Wei,Wang Xumei,Lu Jin-Jian,He Ping,Zhang Hongjie,Chen Xiuping
Though palbociclib, a cyclin-dependent kinases 4 and 6 (CDK4/6) inhibitor has been approved for treating breast cancer, two major clinical challenges remain: (i) Triple-negative breast cancer (TNBC) appears to be more resistant to palbociclib, and (ii) Palbociclib-induced senescence-associated secretory phenotype (SASP) has a pro-tumorigenic function. Here we report that combining palbociclib with the STAT3 inhibitor nifuroxazide uncouples SASP production from senescence-associated cell cycle exit. Moreover, we identified nifuroxazide as a CDK2 inhibitor that synergistically promotes palbociclib-induced growth arrest and senescence in TNBC cells. In vitro, the combination of nifuroxazide with palbociclib further inhibited the TNBC cell proliferation and enhanced palbociclib-induced cell cycle arrest and senescence. The modulation of palbociclib-induced SASP by nifuroxazide was associated with the reduction of phosphorylated-STAT3. Nifuroxazide also blocks SASP-dependent cancer cell migration. Furthermore, thermal shift assay and molecular docking of nifuroxazide with STAT3 and CDK2 revealed that it binds to their active sites and acts as a potent dual inhibitor. In vivo, the combination of nifuroxazide with palbociclib suppressed 4T1 tumor growth and lung metastasis. Our data suggest that nifuroxazide enhances the anticancer effects of palbociclib in TNBC by uncoupling SASP production from senescence-associated cell cycle exit and inhibiting CDK2 to promote tumor senescence.
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JOURNAL OF POLYMERS AND THE ENVIRONMENT
·
2023
·
IF 5.3
Zhao Yanna,Hou Wentong,Huang Yuqing,Zhang Ting,Zhang Yingying,Wang Yuqi,Bai Xiaowei
In this paper, a bio-based polyamide imide polymer, PDPD (Polymer of D230, Poly hexa methylene diamide and Dialdehyde vanillin), was synthesized via amine aldehyde condensation reaction. PHMD was prepared from diethyl oxalate (ODE) and 1,6-hexanediamine (HAD), and vanillin was dialyzed to give dialdehyde vanillin (DVA). Then polyether amine D230 was introduced to obtain polyamide prepolymer, and triethyl citrate was used as cross-linking agent to prepare bio-based glass polymer material PDPD through bulk polymerization at high temperature. The thermal stability, glass transition temperature, mechanical properties, and energy storage modulus of PDPD material were investigated through thermogravimetric analysis (TGA), differential scanning calorimetry (DSC), dynamic mechanical analysis (DMA), and stress-strain curve. Results show that PDPD possesses excellent thermal stability and high mechanical strength, with a 5% thermal decomposition temperature at 288 °C, a stress of 7 MPa, and a strain of 117%. The storage modulus of PDPD in DMA testing can reach up to 2907 MPa. The anti-creep performance of PDPD is good, and the creep level after two repairs is within 40%. Self-healing testing revealed that PDPD maintained its performance after self-healing. The synthesized polymer is also recyclable, making it an attractive option for environmentally conscious applications.
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MICROCHIMICA ACTA
·
2023
·Vol. 190
·期 10
·1-10
·
IF 5.7
Wu Yongju,Yi Jinfei,Su Aiwen,Zhang Yanli,Wang Hongbin,Yang Lijuan,Yang Wenrong,Pang Pengfei
T4 polynucleotide kinase (T4 PNK) helps with DNA recombination and repair. In this work, a phosphate pillar[5]arene@palladium nanoparticles@reduced graphene oxide nanocomposite (PP5@PdNPs@rGO)–based electrochemical biosensor was created to identify T4 PNK activities. The PP5 used to complex toluidine blue (TB) guest molecules is water-soluble. With T4 PNK and ATP, the substrate DNA, which included a 5ʹ-hydroxyl group, initially self-assembled over the gold electrode surface by chemical adsorption of the thiol units. Strong phosphate-Zr 4+ -phosphate chemistry allowed Zr 4+ to act as a bridge between phosphorylated DNA and PP5@PdNPs@rGO. Through a supramolecular host–guest recognition connection, TB molecules were able to penetrate the PP5 cavity, where they produced a stronger electrochemical response. With a 5 × 10 −7 U mL −1 detection limit, the electrochemical signal is linear in the 10 −6 to 1 U mL −1 T4 PNK concentration range. It was also effective in measuring HeLa cell lysate–related PNK activities and screening PNK inhibitors. Nucleotide kinase–target drug development, clinical diagnostics, and screening for inhibitors all stand to benefit greatly from the suggested technology, which offers a unique sensing mechanism for kinase activity measurement. Graphical Abstract
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WATER AIR AND SOIL POLLUTION
·
2023
·Vol. 234
·期 10
·1-14
·
IF 2.9
Jia Wenqiang,Pan Xiangrui,Song Jiaying,Wang Jian,Sun Weikai,Xin Yanjun,Yan Qinghua,Zhou Chengzhi,Zheng Hao,Liu Guocheng
Physical disintegration of macro biochars in natural environments produces nano biochars, thus affecting environmental processes (e.g., adsorption, migration) of antibiotic contaminants. However, knowledge about the relationships between adsorption behaviors of antibiotics and unique physicochemical colloidal properties of nano biochar are relatively limited. In this study, a nano biochar (NSD700) was prepared from sawdust-derived biochar (SD700) by a combination method of ball milling, sonication, and centrifugation to contrastively investigate their adsorption for oxytetracycline (OTC). NSD700 had similar chemical structure properties of carbon fraction with SD700, whereas NSD700 possessed greater surface area and porosity. The maximal sorption capacity of OTC on NSD700 was 30.7 mg/g, ~3 times greater than that of SD700 (10.4 mg/g), primarily benefiting from more exposed surfaces and pores. OTC adsorption on NSD700 was inhibited under acidic and alkaline condition, and the inhibiting effect was more significant in comparison with that on SD700. OTC sorption onto NSD700 gradually decreased with increasing concentration of Na + , but was not observed for SD700. Aggregation kinetic of NSD700 varying with Na + concentration demonstrated that the inhibiting effect might be affiliated with the destabilization and aggregation of NSD700, which could obstruct adsorption sites. This study provided insights for antibiotic adsorption characteristics on nano biochar and will help understanding the process and fate of antibiotics in the biochar-remediated environment. Graphical Abstract
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RESEARCH ON CHEMICAL INTERMEDIATES
·
2023
·
IF 3.3
Guo Zhijin,Liang Xuezheng
The novel porous polymer-based solid acid was synthesized via the polycondensation of 1,4-dichloroxylene (DCX) and following sulfonation using chlorosulfonic acid. The polymer structure could be adjusted by introducing naphthalene. The extremely high surface area of 816 m 2 /g and organic framework provided the mass transfer efficiency and hydrophobic surface. The abundant aromatic rings owned enough sulfonation sites, which resulted in high acidity over 2.7 mmol/g. The novel porous polymer-based solid acid showed high activities for the tetrahydropyranylation of alcohols with the yield over 90% in 5 min at room temperature using 1 wt.% catalyst. The novel solid acid could be recycled for 13 times. The efficient procedure for biodiesel synthesis from waste oil on mild condition was also developed using the solid acid. Both the free fatty acids and glycerides were transformed to the biodiesel products with the yield over 99%. The high surface area, high acidity and the organic framework were the key factors for the high activities, which hold great potential in green chemistry.
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JOURNAL OF NANOBIOTECHNOLOGY
·
2023
·Vol. 21
·期 1
·1-13
·
IF 10.2
Hu Shengyun,Xia Kunkun,Huang Xiaobei,Zhao Ye,Zhang Qingqing,Huang Dongdong,Xu Weiyi,Chen Zhengju,Wang Chenfei,Zhang Zhiyong
Colorectal cancer (CRC) is a major cause of cancer-related deaths in humans, and effective treatments are still needed in clinical practice. Despite significant developments in anticancer drugs and inhibitors, their poor stability, water solubility, and cellular membrane permeability limit their therapeutic efficacy. To address these issues, multifunctional CaCO3 nanoparticles loaded with Curcumin (Cur) and protein deacetylase (HDAC) inhibitor QTX125, and coated with hyaluronic acid (HA) (CaCO3@Cur@QTX125@HA), were prepared through a one-step gas diffusion strategy. Dynamic light scattering (DLS), transmission electron microscopy (TEM), and scanning electron microscopy (SEM) showed that CaCO3@Cur@QTX125@HA nanoparticles have uniform spherical morphology and elemental distribution, with diameters around 450 nm and a Zeta potential of − 8.11 mV. The controlled release of Cur from the nanoparticles was observed over time periods of 48 h. Cellular uptake showed that CaCO3@Cur@QTX125@HA nanoparticles were efficiently taken up by cancer cells and significantly inhibited their growth. Importantly, CaCO3@Cur@QTX125@HA nanoparticles showed specific inhibitory effects on CRC cell growth. Encouragingly, CaCO3@Cur@QTX125@HA nanoparticles successfully internalized into CRC patient-derived organoid (PDO) models and induced apoptosis of tumor cells. The multifunctional CaCO3@Cur@QTX125@HA nanoparticles hold promise for the treatment of CRC.
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危险品化学品经营许可证（带存储）