International Journal of Electrochemical Science
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2023
·
IF 1.5
Zhentao Li,Yanhui Fu,Qingsheng Zhu,Saijin Wei,Jie Gao,Yifu Zhu,Ting Xue,Ling Bai,Yangping Wen
Phosphorene (BP) is a rapidly rising star in two-dimensional (2D) layered nanomaterials beyond graphene, and its urgent issues of ambient instability still have to be resolved. Here, the bimetallic Pt-Pd nanoparticle on the BP surface was employed as multifunctional chemical and biochemical sensing platform for electrocatalytic detection of p -aminophenol (PAP), β-galactosidase (β-Gal), and Escherichia coli ( E. coli ). The BP-supported Pt-Pd nanoelectrocatalyst obtained by the electrodeposition of bimetallic Pt-Pd nanoparticle onto the BP surface was very stable in aqueous buffer containing oxygen, which displayed good voltammetric responses for PAP in a linear range of 0.02 -5 μmol·L -1 with a detection limit (LOD) of 1.41 × 10 −8 M and sensitivity 115.81 μA·μM -1 ·cm -2 . PAP could be obtained by the biochemical reaction of β-Gal in the presence of substrate PAP-β-galactopyranoside, which could realize for biochemical sensing of β-Gal in a linear range of 0.2 -0.8 mU·L -1 with a LOD of 9.35 nU·L -1 and sensitivity of 4.77 μA (mU·L -1 ) -1 ·cm -2 . The β-Gal is an indicator of total coliforms represented by E. coli that are induced by isopropyl-β-thiogalactopyranoside, which could applied for biochemical sensing of E. coli in a linear range of 6 × 106 –1.6 × 108 cfu·mL-1 with LOD of 6.65 × 10 5 cfu·mL -1 and sensitivity of 2.44 μA·(cfu·mL -1 ) -1 ·cm -2 . This work will provide experimental support for multifunctional chemical and biochemical sensing platform based on 2D layered nanomaterials decorated with bimetallic nanoelectrocatalysts.
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International Journal of Electrochemical Science
·
2023
·
IF 1.5
Li Zheng,Chao-Rui Zhang,Dan Zhao,Xuan Wanga,Ting-Ting Du
A β-Ni(OH) 2 flakes modified carbon paste electrode [Ni(OH) 2 /CPE] has fabricated for hydrazine sensing. The β-Ni(OH) 2 flakes derived from nickel hexacyanoferrate (NiHCF) through a facile in situ electrochemical method and characterized by Fourier transform infrared spectrometry, X-ray diffraction, and scanning electron microscopy. Meanwhile, the electrochemical performances of the modified electrode studied by electrochemical impedance spectroscopy, chronocoulometry, cyclic voltammetry, and linear sweep voltammetry. Owing to the large active surface area and enhanced electron transfer efficiency of β-Ni(OH) 2 flakes, the Ni(OH) 2 /CPE showed enhanced catalytic activity toward the oxidation of hydrazine compared with NiHCF/CPE and unmodified CPE. Under the optimized conditions, the amperometric current presented linear dependence on the concentration of hydrazine in the ranges of 0.1 ~ 600 μM with the detection limit of 0.04 μM. Furthermore, the sensor has applied for the determination of hydrazine in water samples.
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ONCOLOGY REPORTS
·
2023
·Vol. 50
·期 2
·1-13
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IF 4.2
Xiao Wang,Simin Rong,Yunxiao Sun,Baohui Yin,Xiancong Yang,Xiaoqing Lu,Hongfang Sun,Yunfei Yan,Guangbin Sun,Yan Liang,Pingyu Wang,Shuyang Xie,Youjie Li
Imatinib resistance in chronic myelogenous leukemia (CML) is a clinical problem. The present study examined the role of N‑Myc downstream regulatory gene 3 (NDRG3) in imatinib resistance in CML. Quantitative PCR demonstrated that NDRG3 was highly expressed in patients with CML. Cell Counting Kit (CCK)‑8 experiments proved that NDRG3 promoted the proliferation of K562 CML cells and enhanced imatinib resistance. Dual‑luciferase assay showed that microRNA (miR)‑204‑5p inhibited expression of NDRG3 and immunofluorescence experiments showed that NDRG3 promoted accumulation of β‑catenin in the nucleus, thereby increasing the expression of downstream drug resistance‑ and cell cycle‑associated factors (c‑Myc and MDR1). At the same time, cell proliferation experiments showed that β‑catenin played a role in cell proliferation and drug resistance. Co‑transfection with small interfering (si)‑β‑catenin partially reversed the effect of NDRG3. This finding indicated that NDRG3 plays an important role in imatinib resistance and miR‑204‑5p and β‑catenin are involved in the biological behavior of NDRG3. The present results provide theoretical support for overcoming drug resistance in CML.
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AAPS PHARMSCITECH
·
2023
·Vol. 24
·期 5
·1-11
·
IF 3.3
Zhang Ying,Chen Jiacai,Zhang Zaiyong,Zhu Huajie,Ma Wuzhen,Zhao Xiangyu,Wang Manli,Wang Caifen,Chen Weidong,Naeem Abid,Zhang Jiwen,Guo Tao,Wu Li
Cyclodextrin metal-organic frameworks (CD-MOFs) exhibit a high structural diversity, which contributes to their functional properties. In this study, we have successfully synthesized a novel type of β-cyclodextrin metal-organic framework (β-CD-POF(I)) that exhibits excellent drug adsorption capacity and enhances stability. Single-crystal X-ray diffraction analysis revealed that β-CD-POF(I) possessed the dicyclodextrin channel moieties and long-parallel tubular cavities. Compared with the reported β-CD-MOFs, the β-CD-POF(I) has a more promising drug encapsulation capability. Here, the stability of vitamin A palmitate (VAP) was effectively improved by the solvent-free method. Molecular modeling and other characterization techniques like synchrotron radiation Fourier transform infrared spectroscopy (SR-FTIR), differential scanning calorimetry (DSC), powder X-ray diffraction (PXRD), thermogravimetric analysis (TGA), and nitrogen adsorption isotherm were applied to confirm that the VAP was successfully encapsulated into the channel formed by the dicyclodextrin pairs. Furthermore, the mechanism of stability enhancement for VAP was determined to be due to the constraint and separation effects of β-CD pairs on VAP. Therefore, β-CD-POF(I) is capable of trapping and stabilizing certain unstable drug molecules, offering benefits and application possibilities. Graphical abstract
One kind of cyclodextrin particle with characteristic shapes of dicyclodextrin channel moieties and parallel tubular cavities, which was synthesized by a facile process. Subsequently, the spatial structure and characteristics of the β-CD-POF(I) were primarily confirmed. The structure of β-CD-POF(I) was then compared to that of KOH-β-CD-MOF, and a better material for vitamin A palmitate (VAP) encapsulation was determined. VAP was successfully loaded into the particles by solvent-free method. The arrangement of spatial structure made cyclodextrin molecular cavity encapsulation in β-CD-POF(I) more stable for VAP capture than that of KOH-β-CD-MOF.
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Frontiers in Endocrinology
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2023
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IF 5.2
Tingwan Du,Lian Xiang,Jingjing Zhang,Chunmei Yang,Wenxin Zhao,Jialu Li,Yong Zhou,Ling Ma
Introduction The study aimed to explore the association of serum 25(OH)D 3 and hepatic steatosis in non-alcoholic fatty liver disease (NAFLD) patients and to determine whether the effect of vitamin D (VD) is mediated by activation of the peroxisome proliferator-activated receptor α (PPARα) pathway. Methods The study contained a case-control study, in vivo and in vitro experiments. A case-control study was conducted to compare serum parameters between NAFLD patients and controls and to evaluate the association of 25(OH)D 3 and NAFLD. In vivo study, male Wistar rats were randomly divided into control and model groups, fed a standard chow diet and a high-fat diet (HFD), respectively, for 7 weeks to generate an NAFLD model. Then, the rats were treated with VD and a PPARα antagonist (MK886) for 7 weeks. Tissue and serum were collected and assessed by biochemical assays, morphological analysis, histological analysis, and western blot analysis. In vitro , HepG2 cells were incubated with oleic acid (OA) to induce steatosis, which was evaluated by staining. HepG2 cells were pretreated with MK886 followed by calcitriol treatment, and differences in lipid metabolism-related proteins were detected by western blot. Results NAFLD patients were characterized by impaired liver function, dyslipidemia, and insulin resistance. Serum 25(OH)D 3 was negatively associated with alanine aminotransferase (ALT) in NAFLD. VD deficiency was a risk factor for patients with no advanced fibrosis. Adequate VD status (25(OH)D 3 >20 ng/mL) had a protective effect in patients after adjustment for confounding variables. NAFLD rats showed hyperlipidemia with severe hepatic steatosis, systematic inflammation, and lower serum 25(OH)D 3 . VD treatment ameliorated hepatic steatosis both in NAFLD rats and OA-induced HepG2 cells. Further, MK886 inhibited the anti-steatosis effect of VD. Conclusion The study revealed that an adequate VD level may act as a protective factor in NAFLD and that VD may alleviate hepatic steatosis via the PPARα signaling pathway.
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Reaction Kinetics Mechanisms and Catalysis
·
2023
·Vol. 136
·期 2
·771-786
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IF 1.8
Su Haitang,Yang Zhixiu,Xiao Guomin,Gao Lijing,Wei Ruiping,Zhang Jin
A series of cost-effective (X) K 2 CO 3 /γAl 2 O 3 catalysts for efficient conversion of vinyltrimethoxysilane (VTMOS) to vinyltris(β-methoxyethoxy)silane (VTMOEOS) were synthesized. The microstructure and active site of the synthesized catalysts were analyzed by BET, XRD, FT-IR, SEM, and CO 2 -TPD. The results showed that the synthesized catalysts formed K–O–Al strong basic sites while maintaining the original structure, which greatly improved the basic strength of the catalysts. It was found that the 60%K 2 CO 3 /γAl 2 O 3 possessed the highest the catalytic activity under the activation temperature of 600 °C by optimizing the preparation conditions of K 2 CO 3 /γAl 2 O 3 . Moreover, the optimal reaction process conditions were also determined by optimizing the experimental reaction temperature, reaction time, catalyst dosage, and reactant molar ratio under (60%, 600 °C) K 2 CO 3 /γAl 2 O 3 catalysis. Under the optimal reaction conditions, the conversion of VTMOS was 92.5% and the yield of VTMOEOS was 65%. Meanwhile, the 60%K 2 CO 3 /γAl 2 O 3 catalyst was satisfactorily reusable and maintained a high catalytic activity after four cycles of catalytic reactions.
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CARBOHYDRATE POLYMERS
·
2023
·
IF 11.2
Hengye Li,Xingwei Han,Wenjie Yu,Lin Zhang,Wenwen Bie,Meijie Wei,Zhongxia Wang,Fenying Kong,Wei Wang
Herein, through knitting benzylated β-cyclodextrin (BnCD) by dimethoxypillar[5]arene (P[5]), porous copolymers (P[5]-BnCDs) containing two kinds of macrocycles were synthesized with yields not <97 %. The molar ratio of P[5]/BnCD greatly influenced the P[5]-BnCDs' porosity and adsorption performance. When the molar ratio of P[5]/BnCD was 4/1, the P[5]-BnCD (4-1), demonstrated a surface area up to 515.95 m 2 /g and showed fast adsorption kinetic , high adsorption capacity and good reusability towards the model organic micropollutants (OMPs). The adsorption fitted well with the pseudo-second-order and the Langmuir models, while the thermodynamic studies revealed spontaneous physisorption process. The adsorption mechanism was dominant by host-guest and hydrophobic interactions and the adsorption at environmentally relevant concentrations experiments showed the practicality and superiority in extraction of the OMPs at μg/L level. This study paves a way for the development of versatile porous organic polymers with multiple macrocycles for efficient removal of OMPs from water.
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Nanomaterials
·
2023
·Vol. 13
·期 4
·775
·
IF 5.3
Hong Su,Jiangping Liu,Yanan Hu,Tianhao Ai,Chenhao Gong,Jichang Lu,Yongming Luo
As a representative sulfur-containing volatile organic compounds (S-VOCs), CH3SH has attracted widespread attention due to its adverse environmental and health risks. The performance of Mn-based catalysts and the effect of their crystal structure on the CH3SH catalytic reaction have yet to be systematically investigated. In this paper, two different crystalline phases of tunneled MnO2(α-MnO2and β-MnO2) with the similar nanorod morphology were used to remove CH3SH, and their physicochemical properties were comprehensively studied using high-resolution transmission electron microscope (HRTEM) and electron paramagnetic resonance (EPR), H2-TPR, O2-TPD, Raman, and X-ray photoelectron spectroscopy (XPS) analysis. For the first time, we report that the specific reaction rate for α-MnO2(0.029 mol g−1h−1) was approximately 4.1 times higher than that of β-MnO2(0.007 mol g−1h−1). The as-synthesized α-MnO2exhibited higher CH3SH catalytic activity towards CH3SH than that of β-MnO2, which can be ascribed to the additional oxygen vacancies, stronger surface oxygen migration ability, and better redox properties from α-MnO2. The oxygen vacancies on the catalyst surface provided the main active sites for the chemisorption of CH3SH, and the subsequent electron transfer led to the decomposition of CH3SH. The lattice oxygen on catalysts could be released during the reaction and thus participated in the further oxidation of sulfur-containing species. CH3SSCH3, S0, SO32−, and SO42−were identified as the main products of CH3SH conversion. This work offers a new understanding of the interface interaction mechanism between Mn-based catalysts and S-VOCs.Keywords:CH3SH decomposition;MnO2;oxygen vacancies;oxygen migration
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Journal of Orthopaedic Translation
·
2023
·
IF 6.6
Fengxiang Pang,Shouchang Ding,Nan Li,Zhipeng Li,Nannan Tian,Chuanjian Shi,Fengwei Zhang,Yongxin Mai,Jinfang Zhang,Junyan Wang
Background Osteosarcoma (OS) is the most common primary malignancy in bone tissues, and effective therapeutics remain absent in clinical practice. Traditional Chinese medicines (TCM) have been used for thousands of years, which provide great insights into OS management. Gallic acid (GA) is a natural phenolic acid enriched in various foods and herbs. Several pharmacological activities of GA such as anti-oxidation and anti-inflammation have been well-established. However, its biological function in OS remains not fully understood. Methods The potential anti-cancer properties of GA were evaluated in 143 B, U2OS and MG63 cells. Its effects on cell growth, cell cycle, apoptosis and migration were examined in these OS cells. The lncRNA H19 and Wnt/β-catenin signaling were detected by qPCR, luciferase activity and Western blotting assays. The in vivo effect of GA on tumor growth was investigated using an orthotopic mouse model. Results In the present study, GA was found to suppress the tumor growth in vitro via inducing cell cycle arrest and apoptosis in OS cells, and inhibit the invasion and metastasis as well. Using the orthotopic animal model, GA was also found to suppress tumorigenesis in vivo . Long noncoding RNA (lncRNA) H19 was demonstrated to be down-regulated by GA, and thus disrupted the canonical Wnt/β-catenin signaling in OS cells. Furthermore, the ectopic expression of H19 rescued the GA-induced suppressive effects on tumor growth and metastasis, and partially reversed the inactivation of Wnt/β-catenin signaling. Conclusions Taken together, our results indicated that GA inhibited tumor growth through an H19-mediated Wnt/β-catenin signaling regulatory axis in OS cells. The translational potential of this article The information gained from this study provides a novel underlying mechanism of GA mediated anti-OS activity, suggesting that GA may be a promising drug candidate for OS patients.
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DRUG DEVELOPMENT AND INDUSTRIAL PHARMACY
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2019
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IF 2.367
Jieqiong Lv,Runcheng Liang,Zihua Xia,Yong Li,Zhufen Lv,Dongzhi Hou,Liping Yu,Gang Chen,Yi Liu,Fan Yang
Purpose: A series of β-CD amphiphilic star-shaped copolymers with exceptional characteristics were synthesized and their potential as carriers for micelles drug delivery was investigated.Methods: A series of amphiphilic copolymers based on β-CD were synthesized by introducing poly (acrylic acid)-co-poly(methyl methacrylate)-poly (vinyl pyrrolidone) or poly (acrylic acid)-co-poly(methyl methacrylate)-co-poly(monoacylated-β-CD)-poly (vinyl pyrrolidone) blocks to the primary hydroxyl group positions of β-CD. The micellization behavior of the copolymers, the synthesis conditions, characteristics, drug release in vitro and tissue distribution of vinpocetine (VP) micelles in vivo were investigated.Results: Around 60 types of β-CD amphiphilic star-shaped copolymers were successfully synthesized and the critical micelle concentration ranged from 9.80 × 10−4 to 5.24 × 10−2g/L. The particle size, drug loading and entrapment efficiency of VP-loaded β-CD-P4 micelles prepared with optimal formulation were about 65 nm, 21.44 ± 0.14%, and 49.05 ± 0.36%, respectively. The particles had good sphericity. The cumulative release rates at 72 h of VP-loaded β-CD-P4 micelles in pH 1.0, pH 4.5, pH 6.5, or pH 7.4 media were 93%, 69%, 49%, and 43%, respectively. And, the lung targeting efficiency of VP-loaded β-CD-P4 micelles was 8.98 times higher than that of VP injection.Conclusion: The VP-loaded β-CD-P4 micelles exhibited controlled-release property, pH-induced feature and lung targeting capacity compared with VP injection, suggesting that the β-CD-P4 copolymers are an excellent candidate for micelles drug delivery.
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FUEL PROCESSING TECHNOLOGY
·
2019
·
IF 4.507
Siquan Xu,Donghui Pan,Feng Hu,Yuanfeng Wu,Huazheng Wang,Yuan Chen,Hui Yuan,Lijing Gao,Guomin Xiao
In the presented work, a series of Cr/β zeolites were prepared and used as bifunctional catalysts with Brønsted and Lewis acidity in the conversion of biomass-derived carbohydrates to 5‑hydroxymethylfurfural (HMF). The physicochemical properties of all catalysts were characterized by ICP-AES, N 2 physisorption , solid-state 27 Al NMR, XRD, SEM-EDX, UV–Vis, XPS , Raman, NH 3 -TPD and Py-FTIR techniques. It was found that the catalysts with appropriate total acidity, ratio of Lewis to Brønsted acid sites and acid density appear to be beneficial for HMF formation. Under mild reaction conditions, a superior 72% HMF yield with 83% selectivity was achieved from glucose in the H 2 O/THF biphasic system by using the 0.4-Cr/β zeolite catalyst. Moreover, the results of the stability evaluation showed that the catalytic activity of the 0.4-Cr/β zeolite was not significantly lost after four consecutive cycles, and the framework remained intact. Therefore, the catalytic approach proposed in this study is believed to provide potential prospects for the production of HMF from biomass material refining in the future.
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BIOSCIENCE REPORTS
·
2019
·Vol. 39
·期 1
·
IF 2.535
Chen Gang,Chen Weicheng,Ye Ming,Tan Weiqiang,Jia Bing
Neuroblastoma is the most common tumor in children, with a very poor prognosis. It is urgent to identify novel biomarkers to treat neuroblastoma, together with surgery, chemotherapy, and radiation. Human tripartite motif 59 (TRIM59), a member of the TRIM family, has been reported to participate in several human tumors. However, the exact role of TRIM59 in neuroblastoma is unknown. In the present study, real-time PCR and Western blot were used to measure mRNA and protein levels of TRIM59 in four neuroblastoma cell lines and in neuroblastoma tissues. Lentiviruses targeting TRIM59 were used to up/down-regulate TRIM59 expression levels. Cell Counting Kit-8 and Annexin-V/PI were used to analyze cell proliferation and apoptosis in neuroblastoma cell lines. Our data showed that TRIM59 knockdown inhibits cell proliferation while inducing apoptosis in SH-SY5Y and SK-N-SH neuroblastoma cell lines. TRIM59 knockdown up-regulated expression of Bax and Bim and down-regulated levels of Survivin, β-catenin, and c-myc. Interestingly, the inhibition of cell proliferation caused by TRIM59 knockdown could be blocked by LiCl, which is an agonist of Wnt/β-catenin signaling pathway. In contrast, TRIM59 overexpression could increase cell proliferation, up-regulate Survivin, β-catenin and c-myc, down-regulate Bax and Bim, and these effects could be blocked by XAV939, which is an inhibitor of Wnt/β-catenin signaling pathway. In addition, TRIM59 was up-regulated and positively related with β-catenin in neuroblastoma tissues. In conclusion, TRIM59 was up-regulated in neuroblastoma, and TRIM59 knockdown inhibited cell proliferation by down-regulating the Wnt/β-catenin signaling pathway in neuroblastoma.
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