Water
·
2022
·Vol. 14
·期 20
·3288
·
IF 3.53
Fangfang Ding,Qianqian Zhao,Luchen Wang,Juan Ma,Lingmin Song,Danfei Huang
As two environmental pollutants of great concern, polystyrene microplastics (PS-MPs) and nonylphenol (NP) often coexist in the environment and cause combined pollution. Batch adsorption experiments were carried out by varying parameters such as pH, the particle sizes of the PS-MPs, the initial concentration of NP, and metal ion content. The results showed that the particle size of the PS-MPs in the range tested (0.1, 1, 10, 50, and 100 μm) had a significant effect on their NP adsorption capacity. The NP adsorption process of the PS-MPs was best described by the pseudo-second-order kinetic model and the Langmuir isotherm model, while the intraparticle diffusion and Bangham models were also involved in determining the NP adsorption process of 0.1 μm PS-MPs. Both PS-MPs and NP significantly affected cell proliferation, which had been confirmed by reduced cell viability, a blocked cell cycle G1 phase, and elevated apoptosis by affecting the basic cell functions. Furthermore, the negative effects of 0.1 μm PS-MPs on cell proliferation and function were aggravated after the adsorption of NP. Further research on the potential health risks of PS-MPs combined with NP or other environmental contaminants is needed.Keywords:microplastics;nonylphenol;adsorption;desorption;cytotoxicity
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Foods
·
2022
·Vol. 11
·期 23
·3793
·
IF 5.561
Yang Liu,Jiali Lin,Tiantian Cheng,Yangjie Liu,Fuliang Han
Anthocyanins are substances with multiple physiological activities widely present in red wine, but the influence of structure (methylation, hydroxylation, acylation, glycosylation) on the transport remains ill-defined. In the present study, Caco-2 monolayers were used as an in vitro model of the absorptive intestinal epithelium to transport different types of anthocyanin samples. Results showed that both methylation and acetylation promote the level of transport. Monoglycoside standard exhibited higher transport amount and rate compared to diglycoside standard while the transport level of the monoglycoside mixture was unexpectedly lower than that of the diglycoside mixture. Caco-2 monolayers appeared to be more capable of transporting the single standard than the mixed standard. Meanwhile, the transport of anthocyanins in Caco-2 cell model showed time- and concentration-dependent trends. Anthocyanin treatment had a greater effect on sodium-dependent glucose transporter 1 (SGLT1) mRNA expression than glucose transporter 2 (GLUT2), and significantly down-regulated the protein expression of SGLT1. Although the low bioavailability of anthocyanins requires much more research, further evidence of the role of structure is provided.Keywords:wine;anthocyanins;Caco-2 cell monolayer;absorption;SGLT1;GLUT2
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高影响因子
DALTON TRANSACTIONS
·
2022
·Vol. 51
·期 22
·8832-8839
·
IF 4.569
Yihao Gao,Jie Bai,Tianning Zhou,Yaqiong Gong
In recent years, Co-based metal–organic frameworks (Co-MOFs) have received significant research interest because of their large specific surface area, high porosity, tunable structure and topological flexibility. However, their comparatively weak electrical conductivity and inferior stability drastically restrict the application of Co-MOFs in the synthesis of electrocatalysts. In this study, ZIF-67 was grown on nickel foam by a room temperature soaking method, and then Ir–Co(OH)2@ZIF-67/NF was assembled by a hydrothermal method. The prepared Ir–Co(OH)2@ZIF-67/NF nanosheets exhibit remarkable conductivity, larger electrochemical active surface area and wider electron transport channels. Only ultralow overpotentials of 198 mV, 263 mV, and 300 mV were needed for Ir–Co(OH)2@ZIF-67/NF to reach the current densities of 10 mA cm−2, 50 mA cm−2, 100 mA cm−2, meanwhile, no obvious degradation of the current density at 10 mA cm−2 was observed for about 16 h. This work may provide a promising strategy for developing high-performance MOF-derived materials as electrocatalysts for the OER under alkaline conditions.
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高影响因子
RESEARCH IN MICROBIOLOGY
·
2022
·
IF 3.946
Yuanyuan Song,Ziqi Wang,Yijing Long,Yang Mao,Feng Jiang,Yuanyuan Lu
Candida albicans can form biofilm on biotic and abiotic surfaces of medical implants to cause superficial and systemic infections under specific condition. The formation of hyphae and matrix of C. albicans are considered as probable virulence factors . We assessed the inhibitory activities of 26 anthraquinones against C. albicans biofilm formation, which were substituted by different functional groups including hydroxyl groups, amino groups, carboxyl groups, alkyl groups, and glycoside groups at C1- or C2-position. Among them, anthraquinones without substituents at other positions but only an alkyl group attached to C2-position, namely 2-alkyl-anthraquinones were determined to have significant anti-biofilm activities. Furthermore, 2-ethylanthraquinone can significantly affect genes related to extracellular matrix ( PMT6 and IFD6 ), and hyphal formation ( HWP1 , ECE1 and EFG1 ), leading to the disrupted formation of biofilm, by detail transcriptomics analysis. We believed that 2-ethylanthraquinone could inspire more discoveries of anti-biofilm agents against C. albicans .
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PubMed
高影响因子
Molecular Catalysis
·
2022
·
IF 5.089
Pengxin Yu,Guangyu Sun,Lu Xiong,Boying Zheng,Teng Li,Jun Jiang,Yantao Wang,Weiran Yang
Salicylic acid, generally produced by chemical synthesis based on petrochemical products, is a vital organic acid and widely used in the pharmaceutical synthesis. This work developed a new and green route for the production of salicylic acid from biomass-derived 2-furoic acid in two steps. Firstly, 2 mmol 2-furoic acid was creatively converted to 2,3-benzofuran using HZSM-5 (Si/Al=130) at 280 °C for 1.5 h under 100 psi N 2 ; and then the oxidation of 2,3-benzofuran to salicylate was conducted with tert‑ butyl hydroperoxide under basic condition without metal catalyst at 120 °C for 1 h. The stability of the catalyst for the first step and the possible reaction pathway for the second step based on the control experiments was properly envisioned. Regardless of limited salicylic acid yield (16%) from 2-furoic acid, this work paves a potentially feasible pathway for the preparation of drugs from biomass.
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Biosensors-Basel
·
2022
·Vol. 12
·期 2
·103
·
IF 5.743
Zhanwei Liang,Tao Peng,Xueshima Jiao,Yang Zhao,Jie Xie,You Jiang,Bo Meng,Xiang Fang,Xiaoping Yu,Xinhua Dai
Neutralizing antibody (NAb) is a family of antibodies with special functions, which afford a degree of protection against infection and/or reduce the risk of clinically severe infection. Receptor binding domain (RBD) in the spike protein of SARS-CoV-2, a portion of the S1 subunit, can stimulate the immune system to produce NAb after infection and vaccination. The detection of NAb against SARS-CoV-2 is a simple and direct approach for evaluating a vaccine’s effectiveness. In this study, a direct, rapid, and point-of-care bicolor lateral flow immunoassay (LFIA) was developed for NAb against SARS-CoV-2 detection without sample pretreatment, and which was based on the principle of NAb-mediated blockage of the interaction between RBD and angiotensin-converting enzyme 2. In the bicolor LFIA, red and blue latex microspheres (LMs) were used to locate the test and control lines, leading to avoidance of erroneous interpretations of one-colored line results. Under the optimal conditions, NAb against SARS-CoV-2 detection carried out using the bicolor LFIA could be completed within 9 min, and the visible limit of detection was about 48 ng/mL. Thirteen serum samples were analyzed, and the results showed that the NAb levels in three positive serum samples were equal to, or higher than, 736 ng/mL. The LM-based bicolor LFIA allows one-step, rapid, convenient, inexpensive, and user-friendly determination of NAb against SARS-CoV-2 in serum.Keywords:neutralizing antibody;latex microspheres;lateral flow immunoassay;SARS-CoV-2;receptor binding domain
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PubMed
高影响因子
TEXTILE RESEARCH JOURNAL
·
2022
·
IF 2.455
Jinyu Wang,Xiangshun Li,Liming Wang,Xiaohong Qin,Yunhua Cui
The color of textiles plays a crucial role in meeting humans’ aesthetics needs and pleasure. Nanofiber has potential application prospects in textile and apparel due to its superior properties, such as the high specific surface area and porosity. Simultaneously, the coloring property has attracted widespread attention. However, the dyeing of polyacrylonitrile (PAN) nanofibers with reactive dyes remains a challenge due to the lack of reactive groups. Herein, an efficient strategy is proposed to successfully color electrospun PAN nanofibers with CI Reactive Red 2, enabled by introducing polyethyleneimine (PEI). Firstly, through entangling the molecular chain of PEI in solution, PAN is endowed with abundant cationic groups. Then, PAN/PEI nanofibers were obtained by electrospinning technology and further dyed using the reactive dye. More specifically, the different dyeing parameters, such as dyeing time, temperature, concentration and PEI concentration, were optimized. Compared to pure PAN nanofiber, PAN/PEI nanofiber achieved a higher dye uptake (96%) and better color fastness. This work offers an energy-saving and simple manner for improving the dyeing performance of PAN nanofibers using reactive dye.
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高影响因子
JOURNAL OF THE ELECTROCHEMICAL SOCIETY
·
2022
·Vol. 169
·期 2
·024505
·
IF 4.386
Yifan Li,Jing Hu,Haibin Li,Li Chen
CsH5(PO4)2-doped polybenzimidazole (PBI) membranes exhibit good proton conductivity and mechanical properties. However, their application in fuel cells is impeded by poor performance. In this study, a novel method is confirmed to greatly improve the performance of a fuel cell based on a CsH5(PO4)2-doped PBI membrane. By coating phosphoric acid (PA) on the surface of the catalytic layer of the gas diffusion electrode, the interfacial contact between the electrolyte membrane and catalytic layer is improved, thereby reducing the ohmic resistance and improving the fuel cell performance. The cell performance is improved by ∼700%. Moreover, the effects of humidity and temperature on the performance and resistance analysis of membrane electrode assembly with a CsH5(PO4)2—doped PBI membrane are studied. Testing under a temperature range of 160 °C–220 °C and humidity range from dry to 30% relative humidity, a maximum peak power density of 554 mW cm−2 is found at 200 °C and 20% relative humidity. A durability test of the membrane electrode assembly indicates that its performance remains stable in a humid atmosphere but gradually decreases in a dry atmosphere. This means for the long-term operation of the molten proton conductor electrolyte membrane, a humid atmosphere is necessary to inhibit the performance decay.
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Biosensors-Basel
·
2022
·Vol. 12
·期 2
·66
·
IF 5.743
Bo Wu,Zehua Li,Zepeng Kang,Chunling Ma,Haiyan Song,Fuping Lu,Zhiguang Zhu
D-2-hydroxyglutaric acid (D2HG) is overproduced as a result of the D-2-hydroxyglutaric aciduria and relevant cancers, caused by gene mutation. Accurate analysis of D2HG could help rapid diagnosis of these diseases and allow for timely treatment. In this work, a D-2-hydroxyglutarate dehydrogenase fromRalstonia solanacearum(RsD2HGDH) is cloned and recombinantly expressed. This enzyme features the direct electron transfer to chemical electron mediators (such as methylene blue (MB)) in the absence of additional coenzymes. Therefore, NAD+, a natural electron acceptor for the commercial D2HGDH and usually known for being unstable and difficult for immobilization can be avoided in the preparation of biosensors. TheRsD2HGDH and MB are co-immobilized on a two-dimensional material, Ti3C2MXene, followed by drop-coating on the gold screen-printed electrode (AuSPE) to construct a compact and portable biosensor. The D2HG in samples can be catalyzed byRsD2HGDH, where the current change is measured by chronoamperometry at −0.23 V. The biosensor shows a D2HG detection range of 0.5 to 120 µM (R2= 0.9974) with a sensitivity of 22.26 μA mM−1cm−2and a detection limit of 0.1 µM (S/N = 3). The biosensor retains 72.52% performance of its incipient state after 30 days of storage. The samples of D2HG-containing fetal bovine serum and artificial urine were analyzed with the recovery of 99.56% to 106.83% and 97.30% to 102.47% further indicating the great application potential of our portable D2HG biosensor.Keywords:D-2-hydroxyglutaric acid;MXene;biosensor;screen-printed electrode;D-2-hydroxyglutarate dehydrogenase
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高影响因子
CRYSTENGCOMM
·
2022
·Vol. 24
·期 17
·3217-3229
·
IF 3.756
Yang Tao,Xiaoting Liu,Yunping Zhoujin,Panpan Zhou,Sean Parkin,Tonglei Li,Ju Guo,Faquan Yu,Sihui Long
To probe the effect of substitution on the benzoic acid ring on the polymorphism of phenylaminobenzoic acids, five 3-methyl-2-(phenylamino)benzoic acids (MPABAs) were synthesized. A preliminary polymorph screen led to one form for compounds 1–4 and three forms and a solvate (5-I, 5-II, 5-III, and 5-S) for compound 5. This observation is similar to that of the structurally related compounds with no substitution on the benzoic acid ring. All crystal forms were characterized by single-crystal X-ray diffraction, powder X-ray diffraction (PXRD), and FT-IR spectroscopy. The polymorphs of compound 5 were investigated in detail experimentally and theoretically. A conformational scan provided insight into the origin of polymorphism of 5. NBO calculations confirmed the composition and nature of the sigma bond character of the two N–C bonds in 5. Hirshfeld analysis accounted for the major intermolecular interactions contributing to the overall stability of the crystals. It is clear that conformational flexibility alone doesn't lead to polymorphism, and substitution position and pattern are the real determinant of polymorphism in the investigated compounds.
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CATALYSIS LETTERS
·
2022
·Vol. 152
·期 12
·3716-3724
·
IF 2.936
Chen Hong,Li Yingying,Yu Linhao,Wang Sen,Ma Xueli,Wang Can,Li Yongdan
A series of hydroxyapatite (HAP) supports with different Ca/P ratios were synthesized to prepare the MnO x /HAP catalysts. A MnO x /HAP catalyst showed highly efficient conversion of 5-hydroxymethylfurfural (HMF) into 2, 5-diformylfuran (DFF) in toluene solvent under no-alkali condition. 86.4% conversion of HMF with 90.9% selectivity of DFF at 120 °C for 12 h under 1.0 MPa O 2 over the MnO x /HAP-10.0-400 were obtained. The redox of Mn 4+ /Mn 3+ improved the oxidation of 5-HMF to DFF by the lattice oxygen, and the lattice oxygen was replenished by adsorbing O 2 molecules. The reusability tests were found the catalyst could be reused up to four cycles without notable loss of catalytic activity. The MnO x /HAP-10.0-400 was a stable and reusable material for further industrial exploration of DFF in an environmentally friendly way. Graphical Abstract
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FOOD CONTROL
·
2022
·
IF 6.652
Tian-Lin Wu,Yong-Mei Hu,Yu Sun,Zhi-Jun Zhang,Zheng-Rong Wu,Wen-Bin Zhao,Chen Tang,Sha-Sha Du,Ying-Hui He,Yue Ma,Cheng-Jie Yang,Ying-Qian Liu
Isothiocyanates had attracted attention for many years due to their antimicrobial properties. In this study, the antifungal efficiency of eighteen isothiocyanate compounds were evaluated against six plant pathogenic fungi and two molds in vitro . Among them, 2-(4-methoxyphenyl)ethyl isothiocyanate displayed the highest antifungal activity against Aspergillus niger (EC 50 = 4.19 μg/mL). The further studies of its mode of action showed that it may damage the cell morphology and membrane integrity in a dose dependent manner by morphological observation, propyl iodide staining and the release of cellular contents. On the other hand, the decrease of related enzyme activities in tricarboxylic acid cycle suggested that 2-(4-methoxyphenyl)ethyl isothiocyanate may effected energy metabolism. Besides, reactive oxygen species, the content of malondialdehyde, the total antioxidant capacity , the content of glutathione and the kinase of oxidative reactive were changed in fungi treated with 2-(4-methoxyphenyl)ethyl isothiocyanate, compared with those in untreated fungi, which all indicated that 2-(4-methoxyphenyl)ethyl isothiocyanate exhibited antifungal activity against A.niger by inducing oxidative stress.
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