CELLULAR AND MOLECULAR BIOLOGY
·
2023
·Vol. 69
·期 9
·
IF 1.6
Aili Xuan,Mei Yang,Qun Xia,Qian Sun
Asthma is a respiratory inflammatory disease, and nicotinamide adenine dinucleotide phosphate oxidase 4 (NOX4) is involved in the progression of respiratory diseases. However, the role of NOX4 in asthma remains unclear. In the present study, we aimed to explore the effects of NOX4 on airway remodeling and inflammation. NOX4 expression was measured using immunocytochemistry (IHC), western blot, and real-time PCR (qPCR). Lung tissues were stained using the H&E assay. ELISA was used to examine the levels of airway remodeling-related indicators, and qPCR was used to detect airway inflammatory factors. The results indicated that NOX4 is highly expressed in lung tissues, bronchoalveolar lavage fluid (BALF), and serum of OVA-treated mice. Inhibition of NOX4 alleviated OVA-induced airway remodeling and inflammation. Similarly, TGF-β1 was also upregulated in BALF and serum OVA-induced mice. Inhibition of TGF-β1 signaling also improved airway remodeling and inflammation induced by OVA. Moreover, the downregulation of NOX4 inactivated the TGF-β1-Smad2/3 pathway, and TGF-β1 decreased Smad2/3 expression. Moreover, inhibition of the TGF-β1 was enhanced, while TGF-β1 reversed the effects on airway remodeling and inflammation induced by NOX4 inhibition. Taken together, the downregulation of NOX4 improves airway remodeling and inflammation via inactivation of the TGF-β1-Smad2/3 pathway in asthma mice, suggesting that NOX4 may be a therapeutic target for asthma.
✓ 已验证
DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
·
2019
·
IF 8.456
Weiwei Mu,Dandan Jiang,Shengjun Mu,Shuang Liang,Yongjun Liu,Na Zhang
The specific-targeting approach could promote the specificity of diagnosis and the accuracy of cancer treatment. The choice of a specific-targeting receptor is the key step in this approach. Glypican-3 (GPC3) is an oncofetal proteoglycan anchored on the cell membrane. It is overexpressed even in the early stage of hepatocellular carcinoma (HCC), whereas it shows almost no expression in the healthy adult liver. Therefore, GPC3 may be applied as a specific-targeting receptor for HCC theranostics. In this study, a GPC3 specific-targeting theranostics nanodevice, GPC3 targeting peptide (named G12)-modified liposomes co-loaded with sorafenib (SF) and IR780 iodide (IR780), was developed (GSI-Lip), which aims to realize early diagnosis and precise chemo-photothermal therapy of HCC. SF was the first-line chemotherapy drug for the treatment of HCC. IR780 was used for photothermal therapy and near-infrared fluorescence imaging. The evaluation of early diagnosis verified that early-stage tumors (3.45 ± 0.98 mm3, 2 days after 5 × 105 H22 cells’ inoculation in mice) could be clearly detected using GSI-Lip, which was significantly more sensitive than folic acid-modified liposomes (p < 0.01, 32.90 ± 10.01 mm3, 4 days after 1 × 106 H22 cells’ inoculation in mice). The study of the endocytic pathway indicated that specific G12/GPC3 recognition may induce caveolae-mediated endocytosis of GSI-Lip. Notably, the accumulation of GSI-Lip in tumors was significantly increased compared with that observed with folic acid-modified liposomes (p < 0.01). Specific-targeting endowed the precise antitumor effect of GSI-Lip. GSI-Lip showed a higher antitumor efficacy in comparison with folic acid-modified liposomes (inhibition rate: 90.52% vs 84.22%, respectively; p < 0.01). During a period of 21 days, the synergistic chemo-photothermal therapy (GSI-Lip + laser) exhibited a better antitumor effect versus GSI-Lip without laser (inhibition rate: 94.93% vs 90.52%, respectively; p < 0.01). Overall, GPC3-targeted GSI-Lip promoted the sensitivity and specificity of HCC early diagnosis and achieved synergistic efficacy of chemo-photothermal theranostics, which has potential clinical applications. Furthermore, the present study revealed that a more specific-targeting ligand could further improve the efficacy of theranostics against HCC.
✓ 已验证
DOI
PubMed
高影响因子
POLYMER BULLETIN
·
2016
·Vol. 74
·期 7
·2719-2739
·
IF 1.371
Fan Xinjian,Zhang Zhiying,Yue Shujing,Xiao Changfa
A series of novel covalently linked waterborne polyurethane/3-aminopropyltriethoxysilane/multiwalled carbon nanotube (WPU/Si/MWCNT) nanocomposites were synthesized through an optimized copolycondensation of hydroxyls. A multiwalled carbon nanotube (MWCNT) as the inorganic filler was pretreated with a successive two-step modification route for its surface function with hydroxyl groups. 3-Aminopropyltriethoxysilane (APTES) was embedded into the backbone of the WPU molecules, hydrolysis of which generates silanol groups in the polyurethane (PU) matrix at the emulsification stage. Meanwhile, the silanol groups could condense with the hydroxyl groups on the surface of the modified MWCNT, generating the covalent bond between the modified MWCNT and the APTES-embedded PU matrix. The change of surface composition of the MWCNT during surface modification process was characterized by using both Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy. The transmission electron microscope (TEM) was utilized to image morphology of the modified MWCNT. The field emulsion scanning electron microscopy (SEM) micrographs and particle size distribution curves showed that the modified MWCNT was well dispersed in the PU matrix. The thermal stability, water resistance, mechanical properties and dynamic mechanical thermal behaviors of the WPU/Si/MWCNT films were characterized by thermogravimetric analysis, swelling test, tensile test, and dynamic mechanical thermal analysis, respectively. These results revealed that compared to the pristine WPU, the WPU/Si/MWCNT system with APTES and the modified MWCNT possesses outstanding water resistance, high thermal stability and excellent mechanical properties.
✓ 已验证
DOI
高影响因子
RUSSIAN JOURNAL OF APPLIED CHEMISTRY
·
2020
·Vol. 93
·期 3
·380-392
·
IF 0.69
Li Jianbo,Lv Jie,Fu Luoping,Tang Mingjin,Wu Xiaodan
Abstract Inspired by the high-water solubility, photoluminescence and low cytotoxicity of N-doped carbon quantum dots (N-CQDs), fluorescent corrosion inhibitors with size of 4–8 nm were synthesized from disodium ethylenediaminetetraacetate, sulfamic acid, and urotropine salt. We expect that these N-CQDs may possess an excellent protective ability to inhibit the corrosion of carbon steel in aggressive solutions owing to its special functional group. The inhibitory mechanism of the inhibitor on N80 steel in saturated CO 2 3 wt % NaCl solution was studied by weight loss, adsorption curve fitting, electrochemical measurement, and surface morphology and elemental analysis. The results show that corrosion of N80 carbon steel in saturated CO 2 3 wt % NaCl solution is significantly inhibited by the addition of N-CQDs, which is attributed to the formation of its adsorption film, preventing the contact of iron with chloride ions and is an effective corrosion inhibitor.
✓ 已验证
DOI
高影响因子
JOURNAL OF ALLOYS AND COMPOUNDS
·
2020
·
IF 4.65
Zhaoqian Yan,Zhihao Sun,Kaicheng Yue,Anran Li,Lei Qian
Cobalt oxide (CoO) with high theoretical capacity is a potential anode material for lithium ion batteries (LIBs), however its application is limited by high cost and volume expansion. Considering the cheapness of Zn and advantages of bimetallic oxide compounds, CoO/ZnO nanoclusters immobilized on N-doped 3 D reduced graphene oxide (CoO/ZnO-NrGO) were constructed by facile solvothermal and freeze-drying method as the LIBs anode for improving lithium (Li) ions storage. Three-dimensional conductive network structure not only reduced the volume expansion of CoO and ZnO but also accelerated the transfer rate of Li ions. Besides, nitrogen doping provided more active sites for plentiful Li ions storage. Phase composition and microstructure of CoO/ZnO-NrGO were measured by X-ray photoelectron spectroscopy, transmission electron microscopy , Raman spectroscopy , X-ray diffraction and so on. Synchronously, electrochemical property of CoO/ZnO-NrGO was also tested. It was found that the specific capacity of CoO 1.2 /ZnO 0.6 -NrGO 6.78 was 1494 mA h g −1 at 0.1 A g −1 , higher that of other bimetallic oxides such as CoO–MnO, CoO–Co 3 O 4 , CoO–CoFe 2 O 4 and so on. The capacity of CoO 1.2 /ZnO 0.6 -NrGO 6.78 was maintained approximately 600 mA h g −1 at 2.0 A g −1 after 1000 cycles. Taking these advantages into account, composites of bimetallic oxides and carbon material with high specific capacity provide a novel idea for a new generation of LIBs.
✓ 已验证
DOI
高影响因子
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2022
·Vol. 23
·期 10
·5715
·
IF 6.208
He Wang,Jingjing Li,Qian Yang,Lan Wang,Jing Wang,Yaxin Zhang,Yanjing Guo,Rui Li,Ruiqi Zhang,Xiaorong Tao,Bernal E. Valverde,Sheng Qiang,Hazem M. Kalaji,Shiguo Chen
2-Amino-3-methylhexanoic acid (AMHA) was synthetized as a non-natural amino acid more than 70 years ago; however, its possible function as an inducer of plant resistance has not been reported. Plant resistance inducers, also known as plant elicitors, are becoming a novel and important development direction in crop protection and pest management. We found that free AMHA accumulated in the mycelia but not in fermentation broths of four fungal species,Magnaporthe oryzaeand threeAlternariaspp. We unequivocally confirmed that AMHA is a naturally occurring endogenous (2S, 3S)-α-amino acid, based on isolation, purification and structural analyses. Further experiments demonstrated that AMHA has potent activity-enhancing resistance against extreme temperature stresses in several plant species. It is also highly active against fungal, bacterial and viral diseases by inducing plant resistance. AMHA pretreatment strongly protected wheat against powdery mildew,ArabidopsisagainstPseudomonas syringaeDC3000 and tobacco againstTomato spotted wilt virus. AMHA exhibits a great potential to become a unique natural elicitor protecting plants against biotic and abiotic stresses.Keywords:agricultural production;natural product;plant resistance inducer;biopesticide;biotic and abiotic stress
✓ 已验证
DOI
PubMed
高影响因子
MATERIALS SCIENCE FORUM
·
2020
·
IF 0
Hong Qian Dai,Yuan Lu,Zhi Hin Ou,Min Xu
Fe3O4-APTES-CS2 was prepared by modification of Fe3O4 with 3-aminopropyltriethoxysilane (APTES) and carbon disulfide (CS2). Lipase was covalently bonded to Fe3O4-APTES-CS2 to obtain the immobilized lipase Fe3O4-APTES-CS2-lipase. The textural characteristic of Fe3O4-APTES-CS2-lipase was assessed by scanning electron microscopy. The optimal immobilization conditions were 2.5 mg/mL lipase, pH 7.0, 35 °C, 3 h. The loading amount of lipase was 119.0 mg/g carrier. The immobilization efficiency reached 57%. (R)-(+)-N-acetyl-1-methyl-3-amphetamine was synthesized by Fe3O4-APTES-CS2-Li- pase. The conversion, enantiomeric excess of (R)-(+)-N-acetyl-1-methyl-3-amphet-a- mine and E value reached 36.8%, 98.5% and 132, respectivel.
✓ 已验证
DOI
PeerJ
·
2019
·
IF 2.353
Hongli Yin,Ji Ma,Jichun Han,Maoru Li,Jing Shang
Background Quercetin (Qr), isoquercitrin (IQ), and quercetin-3-O-β-D-glucuronide (QG) are powerful phytochemicals that have been shown to exhibit disease prevention and health promotion properties. However, there may exist transformations between Qr, IQ, and QG in vivo . And the pharmacokinetic profiles of Qr, IQ, and QG have not been systematically compared. The pharmacokinetics study would be helpful to better understand the pharmacological actions of them. Methods Herein, we developed a reliable HPLC-MS method to compare the pharmacokinetics of Qr, IQ, and QG after separate (50 mg/kg) oral administration of them in rats, using puerarin as internal standard. The detection was performed using negative selected ion monitoring. This method was validated in terms of selectivity, linearity, precision, accuracy, extraction recovery, matrix effect, and stability; and shows reliabilities in monitoring the pharmacokinetic behaviors of these three compounds. Results Our results showed that after separate oral administration of Qr, IQ, and QG, all of the compounds could be detected in plasma. In addition, QG could be detected in the Qr group; Qr and QG could be measured in the IQ group; and Qr could be found in rat plasma after 1.5 h of QG administration. Moreover, the AUC 0− t of Qr in the; Qr group (2,590.5 ± 987.9 mg/L*min), IQ group (2,212.7 ± 914.1 mg/L*min), and QG group (3,505.7 ± 1,565.0 mg/L*min) was larger than the AUC 0− t of QG in the; Qr group (1,550.0 ± 454.2 mg/L*min), IQ group (669.3 ± 188.3 mg/L*min), and QG group (962.7 ± 602.3 mg/L*min). The AUC 0− t of IQ was the lowest among all groups. Discussion Quercetin, IQ, and QG can all be absorbed into plasma. A mutual transformation exists between Qr and QG, and IQ can be metabolized into Qr and QG in SD rats. These results would provide a meaningful basis for understanding the pharmacological actions of these three compounds.
✓ 已验证
DOI
PubMed
高影响因子
HIPPOCAMPUS
·
2023
·
IF 3.5
Shicheng Jiang,Bei Liu,Kaiwen Lin,Lianjun Li,Rongrong Li,Shuo Tan,Xinyu Zhang,Lei Jiang,Hong Ni,Yuanyuan Wang,Haihu Ding,Jing Hu,Hao Qian,Rongjing Ge
Numerous epilepsy-related genes have been identified in recent decades by unbiased genome-wide screens. However, the available druggable targets for temporal lobe epilepsy (TLE) remain limited. Furthermore, a substantial pool of candidate genes potentially applicable to TLE therapy awaits further validation. In this study, we reveal the significant role of KCNQ2 and KCNQ3, two M-type potassium channel genes, in the onset of seizures in TLE. Our investigation began with a quantitative analysis of two publicly available TLE patient databases to establish a correlation between seizure onset and the downregulated expression of KCNQ2/3. We then replicated these pathological changes in a pilocarpine seizure mouse model and observed a decrease in spike frequency adaptation due to the affected M-currents in dentate gyrus granule neurons. In addition, we performed a small-scale simulation of the dentate gyrus network and confirmed that the impaired spike frequency adaptation of granule cells facilitated epileptiform activity throughout the network. This, in turn, resulted in prolonged seizure duration and reduced interictal intervals. Our findings shed light on an underlying mechanism contributing to ictogenesis in the TLE hippocampus and suggest a promising target for the development of antiepileptic drugs.
✓ 已验证
DOI
PubMed
高影响因子
Journal of Materials Chemistry A
·
2019
·Vol. 7
·期 24
·14681-14688
·
IF 10.733
Yi Peng,Rou Tan,Jianmin Ma,Qiuhong Li,Taihong Wang,Xiaochuan Duan
Lithium vanadium phosphate Li3V2(PO4)3 (LVP)-based cathodes have attracted much attention in pursuit of high-performance lithium-ion batteries (LIBs). In this study, an ionic liquid (IL) has been introduced into the electrospinning process for the first time, successfully leading to the formation of free-standing cathodes built up of LVP nanocubes embedded in N-doped carbon nanofibers (LVP-NC/NCNF). In particular, the IL plays multiple roles in the resulting free-standing cathodes: (i) the IL improves the feasibility of the PAN-based electrospinning system for preparing electrode materials due to its good solubility, resulting in a self-supporting substrate conductive network with good mechanical properties and electrical conductivity; (ii) the IL can act as a structure-directing agent to induce the formation of (100) facet-oriented LVP nanocubes, giving rise to a convenient lithium-ion transport path; and (iii) the IL adsorbed on the surface of LVP nanocubes can form a N-doped dual-phase carbon coating layer during the heat-treatment process, further enhancing the electronic conductivity of the entire electrode. As a result, LVP-NC/NCNF free-standing electrodes exhibit an outstanding electrochemical performance including a high specific capacity, stable cycling performance and superior rate capability. Specially, the LVP-NC/NCNF free-standing cathodes deliver a high discharge capacity of 143.6 mA h g−1 at 5C for a prolonged cycling life of over 1000 cycles. It is highly expected that this facile IL-assisted electrospinning method can be expanded to other polyanion-based cathodes, thus presenting new opportunities for the design and fabrication of high-performance free-standing electrodes for LIBs.
✓ 已验证
DOI
高影响因子
Desalination and Water Treatment
·
2014
·
IF 0.987
Ling Li,Jun Cheng Li,Zhuang Rao,Gong Wu Song,Bin Hu
The adsorpion of methylene blue from aqueous solution on a highly porous metal-organic framework material [Cu3(BTC)2(H2O)3] was studied in conjunction with the adsorption isotherm, kinetics and regenerate of the sorbent. The adsorption isotherms of methylene blue on [Cu3(BTC)2(H2O)3] followed both the Freundlich isotherm and the Langmuir adsorption isotherm. Adsorption kinetics was determined from the experimental data, and the results showed that the adsorption obeyed a pseudo-second-order kinetics. The best oscillate time and the best [Cu3(BTC)2(H2O)3] amount were discussed, and [Cu3(BTC)2(H2O)3] gave high adsorption in only 5 minutes. The used [Cu3(BTC)2(H2O)3] could be regenerated by glycol solution, thus recycled. SEM image and FT-TR spectra of recycled [Cu3(BTC)2(H2O)3] after different adsorption times were compared. The high adsorption capacity and excellent reusability make [Cu3(BTC)2(H2O)3] attractive for the removal of methylene blue from aqueous solution.
✓ 已验证
DOI
高影响因子
Journal of the Iranian Chemical Society
·
2023
·Vol. 20
·期 11
·2703-2712
·
IF 2.4
Zhang Chenglu,Nie Shiru,Ding Yanwei,Liu Chang,Zhang Yanpeng,Zhang Yang,Guo Jinghao
An “off–on–off” fluorescent probe NapEb for high selectivity and reversible response to H 2 O 2 and GSH was synthesized. Inspired by the role of GSH in the human body, the –Se–N– bond in ebselen was successfully used as the recognition site. The probe NapEb is sensitive (LOD = 8.4 µM) and fast for H 2 O 2 (within 200 s). It can be used not only for qualitative and quantitative detection of H 2 O 2 in real samples, but also has been successfully used for the imaging detection of H 2 O 2 in MCF-7 cells with good effect. Moreover, continuous fluorescence recognition of GSH has also achieved significant results, the process is reversible and can be repeated for 5 times. The experimental results show that satisfactory results can also be obtained by structural modification at the 3-position of naphthalimide.
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）