BIORESOURCE TECHNOLOGY
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2015
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IF 4.494
Xuliang Lin,Xueqing Qiu,Duming Zhu,Zihao Li,Ningxin Zhan,Jieyi Zheng,Hongming Lou,Mingsong Zhou,Dongjie Yang
Effect of the molecular structure of lignin-based polyoxyethylene ether (EHL–PEG) on enzymatic hydrolysis of Avicel and corn stover was investigated. With the increase of PEG contents and molecular weight of EHL–PEG, glucose yield of corn stover increased. EHL–PEG enhanced enzymatic hydrolysis of corn stover significantly at buffer pH 4.8–5.5. Glucose yield of corn stover at 20% solid content increased from 32.8% to 63.8% by adding EHL–PEG, while that with PEG4600 was 54.2%. Effect of EHL–PEG on enzymatic hydrolysis kinetics of cellulose film was studied by quartz crystal microbalance with dissipation monitoring (QCM-D) and atomic force microscopy (AFM). An enhancing mechanism of EHL–PEG on enzymatic hydrolysis kinetics of cellulose was proposed. Cellulase aggregates dispersed by EHL–PEG excavated extensive cavities into the surface of cellulose film, making the film become more loose and exposed. After the maximum enzymatic hydrolysis rate, the film was mainly peeled off layer by layer until equilibrium.
研究领域:
生物质转化
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酶工程
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高分子化学
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表面科学
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可再生能源
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DOI
高影响因子
Small
·
2024
·Vol. 21
·期 4
·2410657
·
IF 13
Jia Wang,De Wang,Zelin Zhao,Ganggang Chang,Wei Guo,Jun Xu,Yinyu Xiang,Junrui Li,Junsheng Li
Developing cost-effective ruthenium (Ru)-based HER electrocatalysts as alternatives to commercial Pt/C is crucial for the advancement of proton exchange membrane water electrolysis (PEMWE). However, the strong hydrogen adsorption of Ru-based catalysts restricts its activity. Herein, a strategy is reported to tune the electronic structure and improve mass transfer by implanting Ru atoms onto the (002) facet of two-dimensional zeolitic imidazolate framework-67 (Ru@ L ZIF (002) ) to optimize the d -band center ( ε d ) of Ru and the hydrogen spillover behavior. Benefiting from the ultrathin nanosheet structure and optimized ε d of Ru, the over-strong H adsorption energy is weakened and the electron/mass transfer is facilitated. Ru@ L ZIF (002) exhibits an overpotential of 9.2 mV at 10 mA cm −2 and a long-lasting stability of 35 days at 100 mA cm −2 . The mass activity and price activity of Ru@ L ZIF (002) is 2.9 and 14.7 times higher than Pt/C, respectively. More impressively, Ru@ L ZIF (002) delivers a cell voltage of 2.01 V at a high current density of 4 A cm −2 in PEMWE. The excellent long-term durability of 1200 hours operating at 4 A cm −2 with an ultralow decay rate of 7.5 × 10 −3 mV h −1 has been achieved, making it promising as a cost-effective alternative to Pt/C catalyst for PEMWE applications.
研究领域:
电催化
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材料科学
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能源化学
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纳米材料
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电化学工程
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可持续能源技术
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高影响因子
JOURNAL OF ASIAN NATURAL PRODUCTS RESEARCH
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2026
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IF 1.6
Fen Qiu,Xue-Qin He,Yi-Zhang Li,Chun-Feng Wu,Du-Dong Wei,Hao-Yu Liu,Li-Ba Xu
We established in vivo models of hippocampal oxidative injury to investigate the protective effect of Angelica dahurica polysaccharide (ADP) on the hippocampus. ADP alleviated ovariectomized/D-Galactose (OVX/D-Gal)-induced hippocampal damage and enhanced antioxidant capacity (increased SOD/GSH-Px, decreased MDA). Additionally, ADP activated the Nrf2 pathway (upregulated Nrf2, downregulated NQO1/HO-1) and inhibited apoptosis (upregulated Bcl-2, downregulated Bax/cleaved caspase-3). Untargeted metabolomics revealed that ADP reversed seven oxidative stress-related metabolites, and pathway analysis implicated glycerophospholipid metabolism in its antioxidant mechanism. These results demonstrated that ADP protects against OVX/D-Gal-induced hippocampal oxidative injury by activating Nrf2, enhancing antioxidant capacity, inhibiting apoptosis, and regulating glycerophospholipid metabolism.
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DOI
PubMed
高影响因子
JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY
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2026
·
IF 5.3
Jiake Gao,Yujing Zhang,Rui Qu,Qianqian Xu,Zhenjuan Sun,Liya Cui,Chao Wang
Microtubules assembled from α/β-tubulin heterodimers are critical for cell division and well-established anticancer drug targets, making tubulin polymerization inhibitors a viable route for new chemotherapeutics. Guided by structural analysis of colchicine-site binders and tubulin-ligand computational simulations, we rationally designed and synthesized a series of 6-aryl-1-(3,4,5-trimethoxyphenyl)-1H-pyrazolo[3,4-d]pyrimidines as novel colchicine-binding site tubulin inhibitors. Derivative 9t displayed the strongest antiproliferative potency, with IC₅。 values of 0.065-0.096 μM across tested cancer lines. It exerted minimal toxicity to normal L929 fibroblasts, yielding a selectivity index over 300. Mechanistic assays confirmed 9t suppresses cell-free tubulin polymerization, destroys cellular microtubule architecture, induces persistent G₂/M cell cycle arrest, and activates cancer cell apoptosis. Overall, 9t serves as a promising dual-function tubulin inhibitor with both cytostatic and cytotoxic anticancer effects, meriting further preclinical investigation.
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高影响因子
Molecular Catalysis
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2026
·
IF 4.3
Wenhui Li,Jiajia Chen,Jiaolong Fu,Zhi Chen,Xin Ju,Liangzhi Li
Design and fabrication of multifunctional charge-tunable hydrogel carriers. • Molecular docking reveals hydrogen bonding mechanisms in charge-assisted non-covalent immobilization. • PASP/PNIPAM-immobilized enzymes retain 89.7% initial activity after six cycles. • A novel biocatalytic pathway for D-xylose enables efficient rare sugar production. Immobilization of enzymatic cascades is an important tool for the efficient production of value added products, such as rare sugars from biomass monosaccharides. However, there is a lack of biocompatible and inexpensive immobilization carriers with high reusability in immobilizing multiple enzyme proteins currently. In this study, poly-N-isopropylacrylamide (PNIPAM) hydrogels were fabricated and employed to immobilize the enzymatic cascade of glucose isomerase (GI) and D-tagatose-3epimerase (DTE) for the conversion of D-xylose to rare sugar products, D-xylulose and D-ribulose. To attain stable protein attachment and high reusability, PNIPAM microspheres were further grafted by polyaspartic acid (PASP), polyethylene glycol (PEG), polylysine (PL), and anionic polyacrylamide (APAM), obtaining composite hydrogels with tailored surface charges to achieve effective non-covalent immobilization. The enzymatic cascades immobilized by modified PNIPAM hydrogels demonstrated improved thermal stability in the range of 40–70 °C with prolonged enzyme half-lives, and higher conversion of D-xylose. After 6 recycles, PASP/PNIPAM-GI-DTE retained 89.7% initial activity which was superior in enzyme immobilization by PNIPAM. Molecular simulation studies revealed that PASP/PNIPAM formed stable hydrogen bonds with GI and DTE, rationalizing its superior performance. This work proved charge aided immobilization of proteins as a feasible strategy for the production of biomass derived high value chemicals, and provided a practical protocol for industrial scale biomanufacturing. Download: Download high-res image (204KB) Download: Download full-size image
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Food & Function
·
2022
·Vol. 13
·期 3
·1348-1359
·
IF 6.317
Kangliang Sheng,Jian Yang,Yifan Xu,Xiaowei Kong,Jingmin Wang,Yongzhong Wang
Grape seed proanthocyanidin extract (GSPE) that prevents and alleviates the degenerative changes associated with aging has been receiving extensive attention. In our present work, the ageing model was induced by injection of 500 mg kg−1D-galactose daily for a period of eight weeks. The D-galactose-induced ageing mice model was used for evaluating the effect of GSPE on oxidative stress, inflammation levels and gut microbiota composition. D-Galactose induced oxidative damage and inflammation with a significant increase in malondialdehyde contents, myeloperoxidase activities and the levels of TNF-α and IL-1β, as well as a reduction in the activities of glutathione peroxidase and reduced glutathione. Treatment with different doses of GSPE could significantly improve the antioxidant capacity and inflammation levels in the liver and brain, which is accompanied by increased Lachnospiraceae_NK4A136, Lactobacillus, Bifidobacterium, and Akkermansia, as well as decreased Helicobacter and Alistipes. In addition, the high-dose GSPE group exhibited greater potential to delay the ageing process than the low-dose group. Our results also showed that GSPE administration could downregulate the NLRP3 inflammasome signaling pathway for inhibiting inflammation levels in the brain tissue. This study provided a novel strategy to target the gut microbiota with regard to the effect of GSPE administration on alleviating aging-induced alterations via the gut microbiota–liver axis and gut microbiota–brain axis.
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DOI
PubMed
高影响因子
Foods
·
2022
·Vol. 11
·期 3
·386
·
IF 5.561
Ying Xu,Xuan Yu,Jiaqi Gui,Yiqun Wan,Jinping Chen,Ting Tan,Fan Liu,Lan Guo
A rapid and effective ultra-high performance liquid chromatography (UHPLC) method was developed for the determination of five anthraquinones (emodin, physcion, aloe-emodin, rhein, and chrysophanol) inPolygonum multiflorum. The target compounds were ultrasonically extracted with 70% methanol, followed by dispersive solid-phase extraction (d-SPE) with HC-C18 and desorption with acetonitrile. The five anthraquinones were separated on an ACQUITY UPLC®HSS T3 column (2.1 × 100 mm, 1.8 μm) and detected by a photodiode array detector (PDA) at 254 nm. Under the optimized conditions, linear relationships were achieved in the range of 0.3~100 mg/L for emodin, 0.3~40 mg/L for physcion, 0.1~20 mg/L for aloe-emodin, and 0.05~20 mg/L for rhein and chrysophanol. The limits of detection of the five analytes ranged from 0.01 to 0.08 mg/L, and the recoveries were within the range of 82.8~118.4% with an RSD (n= 6) of 1.0~10.3%. The intra-day and inter-day precision (n= 5) of the five targets were in the range of 1.0~1.8% and 3.0~3.1%, respectively. Furthermore, this method was applied to analyses ofPolygonum multiflorumsamples collected from different regions in China with satisfactory results. All the results indicated that this method is suitable for the detection of five anthraquinones inPolygonum multiflorum.Keywords:anthraquinones;Polygonum multiflorum;d-SPE;UHPLC
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PubMed
高影响因子
EUROPEAN JOURNAL OF PHARMACOLOGY
·
2021
·
IF 4.432
Fujing Wang,Rong Hu,Jiaxing Zhang,Tingting Pei,Zhuo'en He,Liliang Ju,Zhongxiao Han,Mingqing Wang,Wei Xiao
Renal fibrosis is the pathological consequence of progressive chronic kidney disease . Although it has been reported that vitamin D3 exerts antifibrotic effects, the underlying mechanisms remain unclear. This study is aimed at investigating the effects and molecular mechanisms in high-dose vitamin D3 treatment on renal fibrosis. A model of chronic kidney disease was established by 5/6 nephrectomy in rats characterised by high levels of serum creatine, urea nitrogen, and urinary protein. Serum 25-dihydroxyvitamin D3, calcium and parathormone levels were measured to evaluate vitamin D levels. Hematoxylin and eosin , periodic acid Schiff and Mallory's Trichrome staining were used to evaluate histopathological changes in rats. Moreover, the expression of vimentin , collagen I, α-smooth muscle actin and E-cadherin were analyzed at molecular and histopathological levels. Our results showed that exposure to vitamin D3 decreased the levels of serum creatine, urea nitrogen and urine protein and restored the homeostasis of calcium and parathormone. Vitamin D3 also downregulated the expression of vimentin, collagen I and α-smooth muscle actin and attenuated renal fibrosis and epithelial to mesenchymal transition in the kidney. Importantly, vitamin D3 treatment increased the expression of the vitamin D receptor and inhibited Transforming growth factor-β1 (TGF-β1)/Smad3 signaling pathway in rats kidneys with chronic kidney disease. Mechanistically, the upregulation of TGF-β1 and phosphorylation of Smad3 induced by vitamin D3 was reversed by activation of the vitamin D receptor. Our findings indicated that vitamin D3 is a potential antifibrotic drug in chronic kidney disease via the vitmin D receptor and inhibiting TGF-β1/Smad3 signaling pathway.
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DOI
PubMed
高影响因子
BIOPROCESS AND BIOSYSTEMS ENGINEERING
·
2021
·Vol. 44
·期 10
·2193-2204
·
IF 3.21
He Song,Wu Xiaomei,Ma Baodi,Xu Yi
The novel Ni-NTA-functionalized magnetic chitosan microspheres (MCS-NTA-Ni) were prepared via amino functionalization of MCS with epichlorohydrin and ethylenediamine, followed by the introduction of the aldehyde groups and NTA in turn, and nickel (II) ions were chelated in the end. MCS-NTA-Ni contained numerous long-armed NTA-Ni surface groups, ensuring high enzyme loading and providing more space and flexibility to attach enzymes and maintain their activity. This microsphere can have highly selective adsorption of his-tagged recombinant protein. The his-tagged recombinant Microbacterium esterase of E. coli BL21 (DE3)/pET21a-EstSIT01 was first immobilized on MCS-NTA-Ni by affinity fixation, giving high immobilization yield (90.1%) and enzyme loading (120 mg/g). Compared with free esterase, the immobilized esterase was found to exhibit higher pH stability and thermal stability. In addition, the immobilized esterase had excellent reusability for the synthesis of key chiral intermediate of d -biotin and the substrate conversion could still keep 100% after 8 cycles continuously.
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PubMed
高影响因子
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
·
2021
·Vol. 109
·期 12
·2506-2515
·
IF 4.396
Jing Wei Jin,Min Zhi Rong,Ming Qiu Zhang,Wing-Leung Wong
β-1,3-D-glucan has been reported to have a series of bioactivities including antitumor, antimicrobial, anti-inflammatory and antioxidative effects; however, its insolubility in neutral aqueous solution significantly restricts the potential application in biological and medicine fields. Herein, a water-soluble aminated β-1,3-D-glucan (AG) was synthesized and the anti-inflammatory effect, cytotoxicity and plasmid DNA (pDNA) binding capacity of AG, serum stability, the particle sizes and zeta potentials of AG/pDNA nanocomposites, and the transfection efficiency and mechanism of action were studied. AG shows no obvious cytotoxicity within the range of working concentration (1–64 μg/ml) and it exerts potent anti-inflammatory effect independent on Dectin-1 and TLR2. AG/pDNA nanocomposites prepared by electrostatic interaction possess an appropriate particle size ranged from 192.8 to 118.4 nm and zeta potentials ranged from 20.880 to 27.16 mV with the N/P ratios from 5 to 100. AG/pDNA nanocomposites at the N/P ratios of 10 and 20 were able to show superior transfection efficiencies in RAW 264.7 cells as a result of their suitable particle size, zeta potential, anti-inflammatory effect, and the specific interaction with pattern recognition receptors (Dectin-1 and TLR2). These results indicate that AG is a potential candidate for DNA delivery system due to its potent anti-inflammatory effect and high transfection efficiency.
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高影响因子
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2020
·Vol. 21
·期 5
·1836
·
IF 4.556
Kaiyue Sun,Yingting Sun,Heyang Li,Dongyao Han,Yuting Bai,Rong Zhao,Zijiao Guo
We aimed to study the effects of an ethyl acetate fraction ofPhysalis alkekengi(PAE) ond-galactose (d-gal)-induced senescence and the underlying mechanism. Firstly, analysis of the phytochemical composition revealed total flavonoids, total phenolics, total saponins, rutin, and luteolin contents of 71.72 ± 2.99 mg rutin equivalents/g, 40.19 ± 0.47 mg gallic acid equivalents/g, 128.13 ± 1.04 mg oleanolic acid equivalents/g, 1.67 ± 0.07 mg/g and 1.61 ± 0.01 mg/g, respectively. The mice were treated withd-gal for six weeks, and from the fifth week, the mice were administered with PAE by gavage once a day for five weeks. We found significantd-gal-induced ageing-related changes, such as learning and memory impairment in novel object recognition and Y-maze, fatigue in weight-loaded forced swimming, reduced thymus coefficient, and histopathological injury of the liver, spleen, and hippocampus. The PAE effectively protected from such changes. Further evaluation showed that PAE decreased the senescence-associatedβ-galactosidase of the liver, spleen, and hippocampus, as well as the oxidative stress of the liver, plasma, and brain. The abundance of flavonoids, phenols, and saponins in PAE may have contributed to the above results. Overall, this study showed the potential application of PAE for the prevention or treatment of ageing-associated disorders.Keywords:Physalis alkekengi;learning and memory;exercise endurance;cellular senescence;oxidative stress
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高影响因子
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
·
2020
·
IF 2.734
Qihong Zhang,Wei Ren,Alexandr V. Dushkin,Weike Su
Olmesartan medoxomil (OM) has been used in treatment of hypertension disease, as a prodrug of olmesartan. However, its clinical application is limited by the low oral bioavailability (~28%). Ternary solid dispersion of OM was prepared with hydroxypropyl- β -cyclodextrin and N -methyl- d -glucamine to improve its oral bioavailability using roll mill ML 007. Its physicochemical characterization was measured by scanning electron microscope, differential scanning calorimetry , powder X-ray diffraction and transform infrared spectroscopy . The solutions properties of OM in ternary solid dispersion were investigated involving the solubility, dissolution profiles, 1 H NMR analysis, phase solubility study and PAMPA study. Instrument analysis results revealed an amorphous solid dispersion had been synthesized without any side-products. It also showed enhanced solubility and dissolution rate in vitro . Pharmacokinetic study demonstrated an improved oral bioavailability in vivo . The rapid storage assay showed that the chemical stability of inclusion complexes had no change. The synthesized OM ternary solid dispersion is promising to be a better alternative to its pure formation in the market with improved oral bioavailability.
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危险品化学品经营许可证（带存储）