BMC BIOLOGY
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2024
·Vol. 22
·期 1
·1-17
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IF 4.4
Yang Yuxuan,Li Wenqing,Sun Kaineng,Sun Siyu,Zhang Yong,Chen Lin,Ni Yangyue,Hou Min,Xu Zhipeng,Chen Lu,Ji Minjun
Background Inflammatory bowel disease (IBD), a persistent gastrointestinal disease, is featured with impaired gut immunity. Previous studies have demonstrated that tuft cells can regulate the intestinal type 2 immune response by activating downstream ILC2 and Th2 cells and repair gut barrier upon invasion of parasitic helminths, bacteria, protozoans, and enteritis through different chemo-sensing receptors, such as bitter taste receptors. Berberine is a widely used in the treatment of diarrhea in clinic, however the mechanism underlying this effect is not clear. In this study, we aim to explore the relationship between berberine and tuft cells in dextran sulfate sodium (DSS) -induced colitis.Results Our data showed that berberine significantly ameliorated DSS-induced colitis and regulating type 2 innate immune lymphocytes (ILC2) and Th2 immune cells via tuft cells in the gut. Furthermore, the effect of berberine on colitis was partially abolished by U73122, a bitter taste receptor inhibitor, suggesting that bitter taste signalling pathway played an important role in the effect of berberine on relieving colitis.Conclusion sBerberine ameliorates dextran sulfate sodium -induced colitis through tuft cells and bitter taste signalling. Our study reveals the unique pharmacological mechanisms of berberine in the context of colitis, laying the foundation for further clinical applications of this compound.
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Chemistry-An Asian Journal
·
2025
·
IF 3.3
Xiaoqian Zhou,Haicheng Song,Xinying Zhang,Lifei Chen,Yingying Chen,Zhenzhen Li,Lingyan Feng
MXene, a two-dimensional nanomaterial, has metal conductivity, high electronegativity, functionalized with surface groups, which make it widely applicable in catalysis and biosensing. However, studies on the principle of enhanced electrochemiluminescence (ECL) by MXene composites and the improvement of their performance in catalyzing the ECL reaction are still in their infancy. In this study, gold nanoparticles (AuNPs) are obtained by mild reductive reduction and loaded in situ on the Ti 3 C 2 T x MXene surface to form the composites (AuNPs@MXene). In oxygenated PBS test buffer, AuNPs@MXene enhance the ECL emission of silver nanoclusters (AgNCs) with aggregation-induced electrochemiluminescence (AIECL) properties as luminophore. Approximately 7.5-fold enhancement of ECL signals is obtained by using two ECL enhancement strategies: an efficient AIECL emitter and a co-reaction accelerator. The special nucleic acid structure with “Three Way Junction (TWJ)” enables an ultra-sensitive detection of microRNA, providing an efficient and ultra-sensitive method for microRNA detection. The biosensor achieves a wide detection range of microRNA-21 from 100 aM to 1 nM, with a low detection limit of 31 aM, and exhibits excellent stability, selectivity and high reproducibility in real samples.
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Nature Communications
·
2024
·Vol. 15
·期 1
·1-20
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IF 14.7
Ouyang Boshu,Shan Caihua,Shen Shun,Dai Xinnan,Chen Qingwang,Su Xiaomin,Cao Yongbin,Qin Xifeng,He Ying,Wang Siyu,Xu Ruizhe,Hu Ruining,Shi Leming,Lu Tun,Yang Wuli,Peng Shaojun,Zhang Jun,Wang Jianxin,Li Dongsheng,Pang Zhiqing
Due to low success rates and long cycles of traditional drug development, the clinical tendency is to apply omics techniques to reveal patient-level disease characteristics and individualized responses to treatment. However, the heterogeneous form of data and uneven distribution of targets make drug discovery and precision medicine a non-trivial task. This study takes pyroptosis therapy for triple-negative breast cancer (TNBC) as a paradigm and uses data mining of a large TNBC cohort and drug databases to establish a biofactor-regulated neural network for rapidly screening and optimizing compound pyroptosis drug pairs. Subsequently, biomimetic nanococrystals are prepared using the preferred combination of mitoxantrone and gambogic acid for rational drug delivery. The unique mechanism of obtained nanococrystals regulating pyroptosis genes through ribosomal stress and triggering pyroptosis cascade immune effects are revealed in TNBC models. In this work, a target omics-based intelligent compound drug discovery framework explores an innovative drug development paradigm, which repurposes existing drugs and enables precise treatment of refractory diseases.
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Materials
·
2025
·Vol. 18
·期 6
·1351
·
IF 3.2
Ming Liu,Zhicun Liu,Xiangkai Qiao,Cheng Chen,Hongtu Guo,Hao Gu,Junbo Li,Tiedong Sun
Nanomotors driven by endogenous enzymes are favored in biology and pharmacy due to their spontaneous driving and efficient biocatalytic activity, and have potential applications in the treatment of clinical diseases that are highly dependent on targeted effects. For diseases such as muscle atrophy, using energy molecules such as ATP to improve cellular metabolism is a relatively efficient treatment method. However, traditional adenosine triphosphate (ATP) therapies for muscle atrophy face limitations due to instability under physiological conditions and poor targeting efficiency. To address these challenges, we developed an endogenous proton-gradient-driven ATP transport motor (ATM), a nanomotor integrating chloroplast-derived FoF1-ATPase with a biocompatible flask-shaped organic shell (FOS). The ATM is synthesized by vacuum-injecting phospholipid-embedded FoF1-ATPase nanothylakoids into ribose-based FOS, enabling autonomous propulsion in acidic microenvironments through proton-driven negative chemotaxis (directional movement away from regions of higher proton concentration). This nanomotor converts proton gradients into ATP synthesis, directly replenishing cellular energy deficits in atrophic tissues. In vitro studies demonstrated high biocompatibility (>90% cell viability at 150 μg/mL) and pH-responsive motility, achieving speeds up to 4.32 μm/s under physiological gradients (ΔpH = 3). In vivo experiments using dexamethasone-induced muscle atrophy mice revealed that ATM treatment accelerated weight recovery and restored normal muscle morphology, with treated mice exhibiting cell sizes comparable to healthy controls (30–40 μm vs. 15–25 μm in untreated). These results highlight the ATM’s potential as a precision therapeutic platform for metabolic disorders, leveraging the natural enzyme functionality and synthetic material design to enhance efficacy while minimizing systemic toxicity.
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ADVANCED FUNCTIONAL MATERIALS
·
2024
·
IF 18.5
Xiaoliang Qi,XinXin Ge,Xiaojing Chen,Erya Cai,Yajing Xiang,Hangbin Xu,Ying Li,Yulong Lan,Yizuo Shi,Hui Deng,Jianliang Shen
Diabetic foot ulcers (DFUs), a serious and increasingly common chronic issue among diabetics, often do not respond well to generalized treatment strategies. Hypoxia and the overexpression of reactive oxygen species (ROS), resulting in inflammatory dysregulation and subsequent imbalance in macrophage phenotypes, are critical factors contributing to the prolonged non-healing of DFU wounds. These two issues often interact in a continuous, problematic cycle. Presently, there is a lack of comprehensive strategies aimed at addressing both of these factors simultaneously to interrupt this detrimental cycle. Herein, an immunomodulatory hydrogel (PHG2) is developed for reshaping the hostile DFU microenvironment. The engineered PHG2 not only removes excess internally-produced ROS but also generates O 2 , with its efficiency further boosted by local hyperthermia (42.5 °C) activated by near-infrared light. Through both in vitro and in vivo studies, along with transcriptomic assessment, it is confirmed that PHG2 disrupts the detrimental feedback loop between ROS and inflammation while also lowering the M1/M2 macrophage ratio. Such discoveries contribute to a significant enhancement in the healing process of injuries that undergo a gradual increase in movement, covering wounds from the back, mouth, to the foot. Ultimately, this method provides an easy, safe, and highly effective solution for treating DFUs.
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BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
·
2025
·
IF 2.2
Haoqi Li,Zijie Cheng,Huimin Li,Yun Yin,Yuqing Li,Tao Chen,Xin Dong,Qingxun Hu,Dan Wu
Nitric oxide (NO) has been highlighted as a key gaseous signaling molecule in the body, playing a central role in various physiological and pathological processes. However, a comprehensive analysis of NO metabolism dynamics in living cells remains a significant challenge. To address this, we have developed and characterized a novel genetically encoded NO fluorescence sensor, GefiNO, to investigate NO metabolism dynamics in living cells and subcellular organelles. In the study, we described the protein detection capabilities and subcellular spatiotemporal localization of GefiNO sensors. By analyzing NO metabolic responses in macrophages of different subtypes, we provide a comprehensive view of NO dynamics at the cellular level. The GefiNO sensor represents a valuable tool for monitoring extensive NO dynamics in living cells and offers potential for uncovering new insights into cellular metabolic mechanisms.
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MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Fangfang Wang,Yawen Liu,Wenping Liu,Huabing Yang,Haiming Hu,Zhigang Zhang,Songlin Liu,Junping Zheng,Hongtao Liu
Plasmonic nanosensors have garnered increasing attention due to their naked-eye visibility and facile preparation. However, the inadequate stability and specificity significantly challenged their widespread application. Here, we hypothesized that capping with double-charged acids and loading in the hydrogel should bring gold nanoparticles (AuNPs) more stability and specificity for sensing small molecules. In the plasmonic nanosensor, aspartic acid-capped AuNPs (Asp-AuNPs) served as both the sensing material and colorant. Compared with conventional AuNPs, Asp-AuNPs exhibited a lower Zeta potential and enhanced selectivity for histamine. Moreover, Asp-AuNPs rapidly responded to histamine in less than 2 min, even after seven weeks of storage. The isothermal titration calorimetry analysis further supported the affinity of Asp-AuNPs for histamine. Using the alginate hydrogels as a matrix for Asp-AuNPs, the hydrogel-based plasmonic nanosensor could detect histamine on the surface of and in the atmosphere around expired fish. In summary, we have developed a novel, durable, and stable hydrogel-based plasmonic nanosensor for contact and contactless freshness detection.
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SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
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2024
·
IF 4.3
Junmei Fan,Ruitao Zhu,Wei Han,Hongfei Han,Liping Ding
Flavonoids are a kind of natural polyphenols which are closely related to human health, and the identification of flavonoids with similar structures is an important but difficult issue. We herein easily constructed a powerful fluorescent sensor ensemble by using surfactant cetyltrimethylammoniumbromide (CTAB) encapsulating two commercially available fluorescent probes ( F1 and F2 ) with multi-wavelength emission. Fluorescence measurements illustrate the present sensor ensemble exhibits turn-off responses to flavones and flavonols but ratiometric responses to isoflavones, owing to different FRET processes. The heat map and linear discriminant analysis (LDA) results show that this single sensor can effectively distinguish 6 flavonoids belong to three subgroups by collecting the fluorescence variation at four typical wavelengths. Moreover, it can be applied to identify different flavonoids even in biofluids like serum and urine, providing potential practical application.
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Heliyon
·
2024
·
IF 3.4
Lin Yang,Lulu Liu,Lingbo Ji,Chenxi Jiang,Zhongrong Jiang,Dongliang Li,Zhen Yang,Wen Cai,Quanwei Zhou,Jinshan Lei,Pinhe Li,Yuhong Jia,Jie Liu,Heng Xu,Jun Hu
Cigars have unique aroma and style characteristics. In order to clarify the differences of aroma components between domestic and imported cigars and the material basis of the stylistic characteristics of different cigars, gas chromatography-mass spectrometry (GC-MS) and sensory evaluation were used to compare and analyze the aroma components in the mainstream smoke of four domestic cigars and two imported cigars. The GC-MS results showed that a total of 97 aroma components were measured in the smoke of the six cigars, and the types of aroma components were similar, but there were differences in their contents. In comparison with those of domestic cigars, imported cigars had suitable nicotine content, and higher contents of phytol, neophytadiene, 3-methylpentanoic acid, and (+)-δ-cadinene. To further explore the differences in the aroma components of the six cigars, GC-MS data combined with chemometrics were used to screen out 14 key aroma components based on P -value (P) < 0.05, Variable Importance Projection (VIP) > 1, and Aroma Activity Values (OAV) > 1. The key aroma components of each cigar were obtained, Snow Dream No. 5: cedrol; Wangguan Guocui: 6-methyl-5-hepten-2-one, pyridine, 2-ethyl-6-methylpyrazine; General Achileus No. 3: p-cresol, 2-methylbutyraldehyde, methyl cyclopentenolone; Montecristo No. 4: cedrol, 2-methylbutyraldehyde, guaiacol, 4-vinylguaiacol, methyl cyclopentenolone; Romeo y Julieta Wide Churchills: cedrol, 2,6-dimethylpyrazine, 2-ethyl-6-methylpyrazine, 2-heptanone, phenethyl alcohol; Great Wall No. 2: p-cresol, phenethyl alcohol, geranylacetone, methyl cyclopentenolone, dihydroactinidiolide. The odor descriptors of these compounds were consistent with the aroma profiles that were prominent in the senses of each cigar. This experiment initially explored the differences in aroma composition and style characteristics of cigars and provided data to support the quality improvement of domestic cigars.
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BIOSENSORS & BIOELECTRONICS
·
2025
·
IF 10.5
Yawen Liu,Fangfang Wang,Haiming Hu,Zhigang Zhang,Huabing Yang,Xiaowei Yao,Hongtao Liu,Junping Zheng
Early diagnosis is crucial for improving the prognosis of patients with metabolic diseases. In this study, we developed an innovative, multiplexed, and user-friendly paper-based plasmonic nanosensor by integrating previously established FeHOAuC (Fe 2+ -catalyzed H 2 O 2 prevents the aggregation of AuNPs by oxidizing cysteine) label-free plasmonic nanosensor. Initially, we prepared a paper art with designated sampling and colorimetric sections by applying polydimethylsiloxane onto cellulose and nitrocellulose papers. Subsequently, we fabricated and optimized the oxidase-coupled FeHOAuC system on the paper platform. The proposed nanosensor's sensitivity, specificity, and feasibility were evaluated using a quantitative color algorithm. In this sensor, pre-loaded oxidases convert target analytes into H 2 O 2 , which subsequently induces a color change in AuNPs by oxidizing cysteine under the catalytic action of Fe 2+ . This paper-based sensor can quantitatively measure glucose, cholesterol, uric acid, and lactate within 40 minutes. The limit of detection of 5–10 μM, combined with its demonstrated specificity, makes it highly suitable for the early diagnosis of related metabolic diseases. Importantly, through a straightforward dropping procedure and a smartphone camera, the plasmonic nanosensor can distinguish disease-related small molecules in real serum samples. In conclusion, the proposed paper-based plasmonic nanosensor device exhibited favorable performance with simple operation, presenting significant potential for domiciliary early diagnosis of multiple metabolic diseases.
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JOURNAL OF NANOBIOTECHNOLOGY
·
2024
·Vol. 22
·期 1
·1-15
·
IF 10.6
Li Jingchao,Wang Tingting,Shi Yuanfei,Ye Zichen,Zhang Xun,Ming Jiang,Zhang Yafei,Hu Xinyan,Li Yun,Zhang Dongsheng,Xu Qianhe,Yang Jun,Chen Xiaolan,Liu Nian,Su Xinhui
Radionuclides internal radiotherapy (RIT) is a clinically powerful method for cancer treatment, but still poses unsatisfactory therapeutic outcomes due to the hypoxic characteristic of tumor microenvironment (TME). Catalase (CAT) or CAT-like nanomaterials can be used to enzymatically decompose TME endogenous H 2 O 2 to boost TME oxygenation and thus alleviate the hypoxic level within tumors, but their effectiveness is still hindered by the short-lasting of hypoxia relief owing to their poor stability or degradability, thereby failing to match the long therapeutic duration of RIT. Herein, we proposed an innovative strategy of using facet-dependent CAT-like Pd-based two-dimensional (2D) nanoplatforms to continuously enhance RIT. Specifically, rationally designed 2D Pd@Au nanosheets (NSs) enable consistent enzymatic conversion of endogenous H 2 O 2 into O 2 to overcome hypoxia-induced RIT resistance. Furthermore, partially coated Au layer afford NIR-II responsiveness and moderate photothermal treatment that augmenting their enzymatic functionality. This approach with dual-effect paves the way for reshaping TME and consequently facilitating the brachytherapy ablation of cancer. Our work offers a significant advancement in the integration of catalytic nanomedicine and nuclear medicine, with the overarching goal of amplifying the clinical benefits of RIT-treated patients. Graphical abstract
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Frontiers in Sustainable Food Systems
·
2024
·
IF 3.7
Ai-Jun Huang,Xiu-Xiu Dong,Shu Tan,Kai Chen,Meiling Zhang,Bingrong Li,Hao Deng,Fan He,Hui Ni,Hongwu Wang,Zi-Jian Chen
Sample pretreatment is an essential procedure in pesticide analysis, as the matrix effect can significantly influence the results. In this study, a covalent organic framework (COF) was synthesized using 1,2,4,5-tetrakis-(4-formylphenyl)benzene (TFPB) and benzidine (BD) to mitigate the matrix effect in vegetable and fruit samples. This COF was then used to develop a solid-phase extraction (CSPE) method. In addition, the COF was used to create a magnetic COF (MCOF) for use in magnetic solid-phase extraction (MSPE). The synthesized COF and MCOF were thoroughly characterized using scanning electron microscopy (SEM) for morphological analysis, Fourier-transform infrared spectroscopy (FT-IR) for chemical bond identification, and N2 adsorption–desorption measurements for porosity and surface area assessment. Key pretreatment parameters such as buffers, dilution rate, sorbent dosage, extraction time, elution solvent, and reuse number were optimized. The developed CSPE and MSPE showed excellent purification ability for the matrix of vegetable and fruit samples. The reuse test demonstrated that the synthesized COF and MCOF can be reused up to 15 times. Moreover, the developed CSPE and MSPE showed acceptable recoveries in spiked recovery tests, suggesting that these pretreatment methods were feasible for sample purification in pesticide analysis.
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