ANALYTICAL CHEMISTRY
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2016
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IF 5.886
Changhui Liu,Weiju Chen,Zhihe Qing,Jing Zheng,Yue Xiao,Sheng Yang,Lili Wang,Yinhui Li,Ronghua Yang
By virtue of its high sensitivity and rapidity, Fenton reaction has been demonstrated as a powerful tool for in vitro biochemical analysis; however, in vivo applications of Fenton reaction still remain to be exploited. Herein, we report, for the first time, the design, formation and testing of Fenton reaction for in vivo fluorescence imaging of hydrogen peroxide (H2O2). To realize in vivo fluorescence imaging of H2O2via Fenton reaction, a functional nanosphere, Fc@MSN-FDNA/PTAD, is fabricated from mesoporous silica nanoparticle (MSN), a Fenton reagent of ferrocene (Fc), ROX-labeled DNA (FDNA), and a cationic perylene derivative (PTAD). The ferrocene molecules are locked in the pore entrances of MSN, and exterior of MSN is covalently immobilized with FDNA. As a key part, PTAD acts as not only the gatekeeper of MSN but also the efficient quencher of ROX. H2O2 can permeate into the nanosphere and react with ferrocene to product hydroxyl radical (·OH) via Fenton reaction, which cleaves FDNA to detach ROX from PTAD, thus in turn, lights the ROX fluorescence. Under physiological condition, H2O2 can be determined from 5.0 nM to 1.0 μM with a detection limit of 2.4 nM. Because of the rapid kinetics of Fenton reaction and high specificity for H2O2, the proposed method meets the requirement for real applications. The feasibility of Fc@MSN-FDNA/PTAD for in vivo applications is demonstrated for fluorescence imaging of exogenous and endogenous H2O2 in cells and mice. We expect that this work will not only contribute to the H2O2-releated studies but also open up a new way to exploit in vivo Fenton reaction for biochemical research.
研究领域:
生物化学
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纳米技术
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生物医学工程
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分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Minggui Gong,Jiajie Su,Ying Chen,Kaixin Zheng,Lijie Yuan,Fangyuan Qin,Huawei Niu,Yong Ye
Heavy metal ions tend to accumulate through the food chain and may cause many serious diseases. As an antioxidant, cysteine (Cys) plays a crucial role in regulating redox homeostasis. In addition, Cys is also widely used in the food industry. Therefore, the development of a convenient, sensitive, and practical tool for detecting Cys is of great significance for investigating the physiological and pathological functions of Cys induced by heavy metal ions. In this paper, a fluorescent probe NYVB with aggregation-induced emission (AIE) activities has been designed and synthesized, which featured good advantages in Cys detection, including favorable selectivity, desirable sensitivity, wide linear range, and low detection limit. The prepared solid-state sensor enabled the ratiometric visual detection of Cys. Probe NYVB has been successfully implemented for testing Cys in real food samples and serum samples with satisfactory recoveries. In addition, probe NYVB has been successfully employed for detecting endogenous and exogenous Cys in SKOV3 cells. More excitingly, probe NYVB has also been used to track the detection of Cys in H 2 O 2 , Hg 2+ , or Cu 2+ -induced redox imbalance. Overall, probe NYVB will open up a new avenue for studying Cys in the amelioration of heavy metal toxicity and has practical applications.
研究领域:
分析化学
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生物化学
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毒理学
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食品科学
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细胞生物学
✓ 已验证
产品关联
DOI
高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
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2019
·
IF 3.973
Wenning Yang,Jia Li,Mingyue Wang,Xiaofan Sun,Yong Liu,Jie Yang,Dickon H.L. Ng
A novel core-shell Fe 3 O 4 /CoFe-LDH (layered double hydroxides) hybrid as a peroxidase mimic for the colorimetric detection of ascorbic acid was first fabricated via a facile two-step route. The resulting Fe 3 O 4 /CoFe-LDH hybrid exhibited much higher peroxidase-like catalytic activity for the oxidation of 3,3′,5,5′- tetramethylbenzidine (TMB) with H 2 O 2 than the pristine Fe 3 O 4 and CoFe-LDH nanosheets owing to the unique hierarchical architecture containing more exposed active sites and the synergistic effect between Fe 3 O 4 and CoFe-LDH. A sensitively and selectively visual sensor for the determination of ascorbic acid (AA) was successfully constructed based on the reduction effect of AA with enediol group on the formed oxidation of TMB, which exhibited a sensitive response to AA in the range of 0.5 ∼ 10 μM with the detection limit of 0.2 μM. Additionally, the Fe 3 O 4 /CoFe-LDH magnetic hybrid could be easily recycled by applying a magnetic field. This work provided a feasible means for the fabrication of magnetic nanomaterials with encouraging prospect in biosensing, environmental monitoring and medical diagnostics.
研究领域:
材料科学
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分析化学
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纳米技术
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生物传感器
✓ 已验证
产品关联
DOI
PubMed
高影响因子
TALANTA
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2019
·
IF 4.916
Zhihong Li,Xinyi Liu,Xiao-Hua Liang,Jianhai Zhong,Liangqia Guo,Fengfu Fu
Two-dimensional WO 3 nanosheets were prepared by ultrasonic exfoliation of bulk WO 3 ·2H 2 O in water and characterized by transmission electron microscopy, atomic force microscopy , X-ray diffraction, X-ray photoelectron spectroscopy, Raman, dynamic light scattering . The nanosheets were discovered to possess the peroxidase-like catalytic activity, which can catalyze the oxidation of 3, 3′, 5, 5′-tetramethylbenzidine by H 2 O 2 . The catalytic mechanism was also investigated by the scavenger experiments. Taking advantage of the peroxidase-like activity of WO 3 nanosheets, a facile colorimetric method for xanthine was developed by combining the oxidation reaction of xanthine catalyzed by xanthine oxidase. The linear range for xanthine was ranged from 25 to 200 μmol L −1 . The limit of detection for xanthine was 1.24 μmol L −1 . The colorimetric method was applied to determine xanthine in urine samples.
研究领域:
纳米材料
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分析化学
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生物催化
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医学诊断
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Advanced Healthcare Materials
·
2024
·
IF 10
Jiaxin Dong,Guanxiong Liu,Yuri V. Petrov,Yujie Feng,Dechang Jia,Vladimir E Baulin,Aslan Yu Tsivadze,Yu Zhou,Baoqiang Li
Iron phosphide/carbon (FeP/C) serving as electrocatalysts exhibit excellent activity in oxygen reduction reaction (ORR) process. H 2 O 2 catalyzed by peroxidase (POD) is similar to the formation of new electron transfer channels and the optimization of adsorption of oxygen-containing intermediates or desorption of products in ORR process. However, it is still a challenge to discover FeP/C with enhanced POD-like catalytic activity in the electrocatalytic database for biocatalysis. The discovery of FeP/carbon dots (FeP/CDs) nanozymes driven by electrocatalytic activity for enhanced POD-like ability is demonstrated. FeP/CDs derived from CDs-Fe 3+ chelates show enhanced POD-like catalytic and antibacterial activity. FeP/CDs exhibit enhanced POD-like activities with a specific activity of 31.1 U mg −1 that is double higher than that of FeP. The antibacterial ability of FeP/CDs nanozymes with enhanced POD-like activity is 98.1%. The antibacterial rate of FeP/CDs nanozymes (250 µg mL −1 ) increased by 5%, 15%, and 36% compared with FeP, Fe 2 O 3 /CDs, and Cu 3 P/CDs nanozymes, respectively. FeP/CDs nanozymes will attract more efforts to discover or screen transition metal phosphide/C nanozymes with enhanced POD-like catalytic activity for biocatalysis in the electrocatalytic database.
研究领域:
材料科学
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纳米技术
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生物化学
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催化
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生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
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2021
·
IF 7.312
Weijie Hua,Yong Kang
Due to the increasingly severe water contamination of antibiotics, high-efficient and green removal of antibiotics from aqueous solution is of great significance to environmental remediation and human health. The combined degradation of tetracycline hydrochloride (TCH) in water by pulsed discharge plasma on water surface (WSPDP) coupled with CaFe 2 O 4 /Bi 2 O 3 composite was investigated. The basic properties of the prepared CaFe 2 O 4 /Bi 2 O 3 composite were analyzed by XRD , SEM, TEM, PL and XPS, which indicated that the CaFe 2 O 4 /Bi 2 O 3 sample was synthesized successfully. Degradation performance revealed that there existed a synergistic effect in the WSPDP + CaFe 2 O 4 /Bi 2 O 3 system, and WSPDP could induce catalysis of CaFe 2 O 4 /Bi 2 O 3 composite. After adding 0.4 g/L 7 wt% CaFe 2 O 4 /Bi 2 O 3 sample to the WSPDP system, the degradation efficiency of TCH improved from 82.9% to 93.8%, and the kinetic constant enhanced from 0.0616 min −1 to 0.0953 min −1 after 30 min of discharge treatment. Furthermore, the effects of pulse peak voltage, pulse frequency, initial pH of solution and air flow rate on TCH degradation were evaluated. The highest TCH degradation efficiency of 97.8% could be obtained at the peak pulse voltage of 24.6 kV, pulse frequency of 50 Hz, initial pH of 3 and air flow rate of 2 L/min within 30 min. OH, h + and O 2 − were the main reactive radicals for TCH degradation in the WSPDP + CaFe 2 O 4 /Bi 2 O 3 system·H 2 O 2 and O 3 were also involved in TCH degradation of the synergistic system, and the addition of CaFe 2 O 4 /Bi 2 O 3 could significantly improve the utilization of H 2 O 2 and O 3 in the solution. The intermediates of TCH degradation were identified by liquid chromatography-mass spectrometry (LC-MS). Finally, the possible degradation pathways and synergistic degradation mechanism of TCH in the WSPDP + CaFe 2 O 4 /Bi 2 O 3 system were proposed.
研究领域:
环境科学
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水处理
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高级氧化过程
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催化
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污染物降解
✓ 已验证
产品关联
DOI
高影响因子
WASTE MANAGEMENT
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2024
·
IF 7.1
Fanbin Zhang,Tinghui Li,Zilong Zhang,Xia Qin,Cuicui Xu
The active graphite felt (GF) catalytic layer was effectively synthesized through a wet ultrasonic impregnation-calcination method, modified with CB and PTFE, and implemented in a pioneering side-aeration electrochemical in-situ H 2 O 2 reactor. The optimal mass ratio (CB: PTFE 1:4) for the modified cathode catalytic layer was determined using a single-factor method. Operating under optimum conditions of initial pH 5, 0.5 L/min air flow, and a current density of 9 mA/cm 2 , the system achieved a remarkable maximum H 2 O 2 accumulation of 560 mg/L, with the H 2 O 2 production capacity consistently exceeding 95 % over 6 usage cycles. The refined mesoporous structure and improved three-phase interface notably amplified oxygen transfer, utilization, and H 2 O 2 yield. Side aeration led to an oxygen concentration near the cathode reaching 20 mg/L, representing a five-fold increase compared to the 3.95 mg/L achieved with conventional bottom aeration. In the final application, the reaction system exhibited efficacy in the degradation of landfill leachate concentrate. After a 60-minute reaction, complete removal of chroma was attained, and the TOC degradation rate surpassed 60 %, marking a sixfold improvement over the conventional system. These results underscore the substantial potential of the system in H 2 O 2 synthesis and environmental remediation.
研究领域:
环境科学
·
电化学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Water
·
2024
·Vol. 16
·期 12
·1658
·
IF 3
Hui Hong,Shiwei Xie,Aoxue Qiu,Yuming Yao,Wenzhe Jiang,Jilei Li,Zihao Wan,Shengjie Xiang,Chenyi Xi,Jingxian Xiao,Na Zhang
This study utilizes a natural air diffusion cathode (ADC) and an ultraviolet lamp to construct a UV/H2O2reactor for the in situ synthesis and activation of H2O2and evaluates its potential application in practical lake water treatment. The results indicate that the reactor exhibits stable treatment performance during a continuous flow experiment of 80 h. The air diffusion cathode maintains an H2O2concentration of above 350 mg·L−1in sodium sulfate electrolyte and shows no decreasing trend. Under the condition of approximately 59% H2O2utilization, the removal rates of COD and TOC are 37.6% and 40.0%, respectively; the rate of reduction of A254is 64.3%; while the total bacterial count removal rate reaches 100%. Large organic molecules in surface water are degraded to small organic molecules and mineralized to inorganic minor molecules. It effectively ameliorates the problem of organic pollution of surface water and effectively kills bacteria and improves the microbiological safety of the water body. Therefore, the UV/H2O2system developed in this study, based on electrochemically produced H2O2, is an effective method for treating micro-polluted surface water.
研究领域:
环境科学
·
水处理工程
✓ 已验证
产品关联
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2024
·
IF 13.3
Binrong Li,Na Li,Zhiwei Guo,Chen Wang,Xuedong Wang,Zhi Zhu,Xu Tang,Pengwei Huo
Polymeric carbon nitride (PCN) is an environmentally friendly photocatalyst for solar-driven H 2 O 2 production, but the activity of original PCN is still not satisfactory. The main limitation is the sluggish exciton dissociation leading to slow generation and conversion of superoxide radical (•O 2 − ) intermediate. Herein, the PCN with dual defect sites and compact interlayer is rationally designed for H 2 O 2 photosynthesis with an ultrahigh rate of 3930.9 μmol g −1 h −1 and rapid •O 2 − yield rate of 1.92 min −1 . The enhanced activity is attributed to effective exciton dissociation during interlayer and intralayer process. Moreover, nitrogen vacancies change the oxygen adsorption configuration (formation of C − O − O − C bridge mode), thus promoting O 2 adsorption and activation. A well-matched linear relationship was established between the concentration of nitrogen vacancies and •O 2 − /H 2 O 2 production. This work not only provides in-depth insights for the photocatalytic H 2 O 2 generation mechanism, but also offers a new paradigm for designing efficient PCN-based photocatalysts for energy conversion.
研究领域:
材料科学
·
光催化
·
能源转换
✓ 已验证
产品关联
DOI
高影响因子
Frontiers of Environmental Science & Engineering
·
2020
·Vol. 14
·期 6
·1-12
·
IF 4.053
Li Wenyue,Chen Min,Zhong Zhaoxiang,Zhou Ming,Xing Weihong
High-concentration industrial wastewater containing N,N -dimethylacetamide (DMAc) from polymeric membrane manufacturer was degraded in Cu 2 O NPs/H 2 O 2 Fenton process. In the membrane-assisted Fenton process DMAc removal rate was up to 98% with 120 min which was increased by 23% over the batch reactor. It was found that ·OH quench time was extended by 20 min and the maximum ·OH productivity was notably88.7% higher at 40 min. The degradation reaction rate constant was enhanced by 2.2 times with membrane dispersion ( k = 0.0349 min −1 ). DMAc initial concentration ( C 0 ) and H 2 O 2 flux ( J p ) had major influence on mass transfer and kinetics, meanwhile, membrane pore size ( r p ) and length ( L m ) also affected the reaction rate. The intensified radical yield, fast mass transfer and nanoparticles high activity all contributed to improve pollutant degradation efficiency. Time-resolved DMAc degradation pathway was analyzed as hydroxylation, demethylation and oxidation leading to the final products of CO 2 , H 2 O and NO 3 − (rather than NH 3 from biodegradation). Continuous process was operated in the dual-membrane configuration with in situ reaction and separation. After five cycling tests, DMAc removal was all above 95% for the initial [DMAc] 0 = 14,000 mg/L in wastewater and stability of the catalyst and the membrane maintained well.
研究领域:
环境科学
·
化学工程
✓ 已验证
DOI
高影响因子
Foods
·
2021
·Vol. 10
·期 2
·419
·
IF 4.35
Pei Hu,Zhentao Sun,Yunwen Shen,Yiwen Pan
Hydrogen peroxide (H2O2) has been reported to be used for the illegal treatment of fishery products in order to obtain “fake” freshness. Residues of H2O2in food may be of toxicology concern. In this study, a nonenzymatic sensor was developed based on Fe@PCN-224 metal–organic frameworks wrapped by Nafion to detect H2O2concentration. The hybrid structure of Fe@PCN-224 was fabricated by incorporated free FeIIIions into the center of PCN-224, which was ultra-stable due to the strong interactions between Zr6and the carboxyl group. Scanning electron spectroscopy images exhibited that Nafion sheets crossed together on the surface of Fe@PCN-224 nanoparticles to form a hierarchical and coherent structure for efficient electron transfer. Electrochemical investigations showed that the Fe@PCN-224/Nafion/GCE possessed good linearity from 2 to 13,000 μM (including four orders of magnitude), low detection limits (0.7 μM), high stability in continuous monitoring (current remained nearly stable over 2300 s) and in long-term measurement (current decreased 3.4% for 30 days). The prepared nanohybrid modified electrode was effectively applied to H2O2detection in three different fishery products. The results were comparable to those measured using photometrical methods. The developed electrochemical method has a great potential in detecting the illegal management of fishery products with H2O2.Keywords:Fe@PCN-224;Nafion;nonenzymatic sensor;hydrogen peroxide;fishery products
研究领域:
食品安全检测
·
电化学分析
·
材料科学
✓ 已验证
DOI
PubMed
高影响因子
Frontiers of Chemical Science and Engineering
·
2023
·Vol. 17
·期 4
·470-482
·
IF 4.5
Wang Yitong,Zhang Yue,Wang Li
Amino-functionalized zirconia was synthesized by the co-condensation method using zirconium butanol and 3-aminopropyltriethoxy silane for the simultaneous removal of various impurities from aqueous 30% H 2 O 2 solution. The results of Fourier transform infrared (FTIR) and Zeta potential showed that the content of N in amino-functionalized zirconia increased with the added amount of 3-aminopropyltriethoxy silane. Accordingly, the removal efficiency of total oxidizable carbon, phosphate and metallic ions from the H 2 O 2 solution increased. The adsorbent with an N content of 1.62% exhibited superior adsorption performance. The removal efficiency of 82.7% for total oxidizable carbon, 34.2% for phosphate, 87.1% for Fe 3+ , 83.2% for Al 3+ , 55.1% for Ca 2+ and 66.6% for Mg 2+ , with a total adsorption capacity of 119.6 mg·g −1 , could be achieved. The studies conducted using simulated solutions showed that the adsorption process of phosphate on amino-functionalized zirconia is endothermic and spontaneous, and the behaviors could be well described by the pseudo-second-order model and Langmuir model with a maximum adsorption capacity of 186.7 mg·g −1 . The characterizations of the spent adsorbents by Zeta potential, FTIR and X-ray photoelectron spectroscopy revealed that the adsorption mechanism of phosphate is predominantly electrostatic attraction by the protonated functional groups and complementary ligand exchange with zirconium hydroxyl groups.
研究领域:
环境科学
·
化学工程
·
材料科学
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）