Food Analytical Methods
·
2013
·Vol. 7
·期 2
·291-297
·
IF 1.969
Wen Shengping,Zhu Xiashi
A simple and rapid method based on dispersive liquid–liquid microextraction with room temperature ionic liquid 1,3-dibutylimidazolium hexafluorophosophate ([BBIM][PF 6 ]) coupled to flame atomic absorption spectrometry was developed for the speciation of Mn(II)/Mn(VII) in tea samples. The main factors affecting dispersive liquid–liquid microextraction were investigated in detail. Mn(II) and total manganese [Mn(II)+Mn(VII)] were quantitatively extracted after adjusting aqueous sample solution to pH 10.0 and 7.0, respectively. Mn(VII) was calculated by subtraction of Mn(II) from total manganese. Under the optimized conditions, the limits of detection (3 σ ) for Mn(II) and Mn(VII) were 0.26 and 1.86 ng/mL, and their relative standard deviations were 2.6 and 4.8 % ( n = 7, c = 25.00 ng/mL). The proposed method was validated by the determination of Mn(II) and Mn(VII) in the certified reference material [GBW(E)08513] of tea sample and different local tea samples with satisfactory results.
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TALANTA
·
2016
·
IF 4.035
Huazi Wang,Lu Hu,Wanzhen Li,Xiaoling Yang,Runhua Lu,Sanbing Zhang,Wenfeng Zhou,Haixiang Gao,Jing Li
A novel in-syringe dispersive liquid-liquid microextraction based on the solidification of ionic liquids (in-syringe SIL-DLLME) was coupled with high-performance liquid chromatography-ultraviolet detector (HPLC-UVD) to detect five benzoylurea insecticides (BUs) in water and tea beverage samples. In this method, the hydrophobic ionic liquid [N 8881 ][PF 6 ] was formed in situ by the metathesis reaction between [N 8881 ]Cl and the anion-exchange reagent KPF 6 to extract the target analytes. The whole extraction procedure was performed in a syringe. The solidified extractant could be separated from the aqueous phase by exposing the emulsified extraction solution to an ice bath and then easily collected by squeezing out the aqueous phase through the prepared NWPP-based needle. Various parameters affecting the extraction efficiency, such as the amount of [N 8881 ]Cl, the molar ratio of [N 8881 ]Cl to KPF 6 , salt addition, cooling time, solution temperature, sample pH and sample volume, were evaluated. Under the optimized conditions, the proposed method was validated with satisfactory results: good linearities with coefficients of determination greater than 0.99 were obtained in the range of 2–500 µg L −1 ; the limits of detection varied between 0.29 and 0.59 µg L −1 ; the recoveries of the five benzoylurea insecticides ranged from 85.93% to 90.52%; and the intra-day ( n =3) and inter-day ( n =3) relative standard deviations were less than 5.36%. Finally, the proposed method was successfully used for the determination of BUs in real water and tea beverage samples.
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Analytical Methods
·
2017
·Vol. 9
·期 13
·2069-2075
·
IF 1.9
Fan Nie,Jiannan Wang,Xin Lu,Fanggui Ye
Bifunctional magnetic nanoparticles with octadecyl and phosphate groups were synthesized by thiol–ene click chemistry and applied for pre-concentration and extraction of organophosphorus pesticides (OPPs). Vinyl phosphonic acid functionalized MNPs (Fe3O4@VPA) behaved as clickable sites and reacted with octadecanethiol (C18-SH) by a thiol–ene click reaction. The resultant material (Fe3O4@VPA@C18) was characterized by scanning electron microscopy, FTIR, transmission electron microscopy and magnetic measurements. The results revealed that the particles exhibited spherical morphology and excellent magnetic response signals. The extraction efficiency was studied using four OPPs as test analytes from tea drink samples and their determination using high performance liquid chromatography was evaluated. Various extraction conditions were investigated in detail. Under optimal conditions, using the recommended method the linear range for the analytes was 0.1–300 μg L−1, the detection limits ranged from 0.01–0.05 μg L−1, and the enrichment factors ranged from 420 to 455. This method was then successfully used to determine OPPs from tea drink samples with desirable recovery ranging from 81.7 to 92.8%. Meanwhile, based on the hydrophobicity of the C18 groups and the selectivity of the phosphate groups, the extraction efficiency of Fe3O4@VPA@C18 for OPPs was superior to that of the commercial adsorbent (Fe3O4@SiO2@C18).
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FOOD CHEMISTRY
·
2024
·
IF 8.5
Xiaojiao Zheng,Xingle Guo,Yawen Zhang,Jiajun Han,Xu Jing,Junxue Wu
A novel, environmentally friendly, and efficient method for determining triazine herbicides in water, tea, and juice was developed by combining magnetic dispersive micro-solid phase extraction (MD-μSPE) with magnetic dispersive liquid-liquid microextraction (MDLLME), followed by high-performance liquid chromatography (HPLC). The pretreatment process, utilized magnetic biochar (MBC) and magnetic deep eutectic solvent (MDES) as the adsorbent and extractant, respectively. Fe(NO 3 ) 3 was loaded onto waste mushroom sticks to prepare MBC via impregnation-pyrolysis, while tri- n -butylphosphine oxide, nonanoic acid, and FeCl 3 were combined through hydrogen bonds to form MDES. This approach significantly reduced environmental impact using waste materials and shortened separation times. The method demonstrated excellent linearity (1–100 μg L −1 ), low detection limits (0.3 μg L −1 ), and good precision (RSDs <6.5 %). Recoveries in water, tea, and juice ranged from 80.1 % to 90.6 %. This combined MD-μSPE-MDLLME approach offers a greener and more efficient extraction process for separating and concentrating pesticides from complex food samples.
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LWT-FOOD SCIENCE AND TECHNOLOGY
·
2025
·
IF 6.6
Ling Zeng,Qiuting Wu,Lingyue Tang,Xiao Yang,Qi An,Hushan Cui,Qing Jin
Herein, the feasibility of supplementing black wheat flour and buckwheat flour for semi-dried noodles (SBBN) preparation was assessed. Due to the high nutrient and water activity (a w ) of SBBN, it is easy to induce microbial contamination. Therefore, a combination of ozonated water and tea polyphenols treatment was used in the study to investigate its effect on microbiological, quality and physio-chemical characteristics. The results indicate that combined treatment significantly reduced the total plate count (TPC) of SBBN, improved its color, cooking and texture characteristics and antioxidant activity, enhance its umami taste, and have no significant impact on its flavor profiles during storage. Additionally, the free sulfhydryl (SH) content was reduced, indicating that the combined treatment promoted protein disulfide cross-linking. Scanning electron microscope (SEM) further confirmed that the binding ability between starch particles and gluten proteins was enhanced. Thus, the introduction of ozonated water and tea polyphenols into the black wheat-buckwheat dough had a significant effect on the optimization of the macro- and micro-characteristics of noodles.
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JOURNAL OF CHROMATOGRAPHY A
·
2017
·
IF 3.981
Guicen Ma,Minglu Zhang,Li Zhu,Hongping Chen,Xin Liu,Chengyin Lu
Amine-functional reduced graphene oxide (amine-rGO) with different carbon chain length amino groups were successfully synthesized. The graphene oxides (GO) reduction as well as amino grafting were achieved simultaneously in one step via a facile solvothermal synthetic strategy. The obtained materials were characterized by X-ray diffraction, Raman spectroscopy, Fourier-transform infrared spectrometry and X-ray photoelectron spectroscopy to confirm the modification of GO with different amino groups. The adsorption performance of catechins and caffeine from tea acetonitrile extracts on different amine functional rGO samples were evaluated. It was found that tributylamine-functional rGO (tri-BuA-rGO) exhibited the highest adsorption ability for catechins and caffeine compared to GO and other amino group functional rGO samples. It was worth to note that the adsorption capacity of catechins on tri-BuA-rGO was 11 times higher than that of GO (203.7 mg g −1 vs 18.7 mg g −1 ). Electrostatic interaction, π-π interaction and surface hydrophilic-hydrophobic properties of tri-BuA-rGO played important roles in the adsorption of catechins as well as caffeine. The gravimetric analysis confirmed that the tri-BuA-rGO achieved the highest efficient cleanup preformance compared with traditional dispersive solid phase extraction (dSPE) adsorbents like primary-secondary amine (PSA), graphitized carbon black (GCB) or C18. A multi-pesticides analysis method based on tri-BuA-rGO is validated on 33 representative pesticides in tea using gas chromatography coupled to tandem mass spectrometry or high-performance liquid chromatography coupled with tandem mass spectrometry. The analysis method gave a high coefficient of determination (r 2 > 0.99) for each pesticide and satisfactory recoveries in a range of 72.1–120.5%. Our study demonstrated that amine functional rGO as a new type of QuEChERS adsorbent is expected to be widely applied for analysis of pesticides at trace levels.
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FOOD RESEARCH INTERNATIONAL
·
2024
·
IF 7
Jinjie Hua,Xizhe Zhu,Wen Ouyang,Yaya Yu,Ming Chen,Jinjin Wang,Haibo Yuan,Yongwen Jiang
Withering is the first and key process that influences tea quality, with light quality being a key regulatory factor. However, effects of withering light quality (WLQ) on transformation and formation pathways of tea aroma and volatile metabolites (VMs) remain unclear. In the present study, four WLQs were set up to investigate their effects on tea aroma and VMs. The results showed that blue and red light reduced the grassy aroma and improved the floral and fruity aroma of tea. Based on GC–MS/MS, 83 VMs were detected. Through VIP, significant differences, and OAV analysis, 13 key differential VMs were screened to characterize the differential impacts of WLQ on tea aroma. Further analysis of the evolution and metabolic pathways revealed that glycoside metabolism was the key pathway regulating tea aroma through WLQ. Blue light withering significantly enhanced glycosides hydrolysis and amino acids deamination, which was beneficial for the enrichment of floral and fruity VMs, such as geraniol, citral, methyl salicylate, 2-methyl-butanal, and benzeneacetaldehyde, as well as the transformation of grassy VMs, such as octanal, naphthalene, and cis -3-hexenyl isovalerate, resulting in the formation of tea floral and fruity aroma. The results provide theoretical basis and technical support for the targeted processing of high-quality tea.
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FOOD RESEARCH INTERNATIONAL
·
2025
·
IF 8
Ziyi Shen,Simeng Zhou,Yaru Zhang,Yuan Zhang,Hongyan Wang,Daxiang Li,Yijun Wang,Zhongwen Xie,Huifang Ge
Large-leaf yellow tea protein-derived oligopeptides (TPP) contain a substantial number of antioxidant peptides that facilitate skin damage repair and may be employed to mitigate skin photoaging. However, the potential alleviating mechanism by which TPP alleviates skin photoaging damage remains insufficiently understood. This study investigated the in vitro antioxidant capacity and cell migration-promoting ability of TPP. Additionally, it utilized ultraviolet (UV)-exposed and intrinsically aged ICR mice, which exhibit signs of skin aging, to assess the effects of an 8-week oral TPP intervention. The findings indicate that TPP exhibits potent free radical scavenging properties, which mitigate oxidative damage in Raw264.7 cells and promote the migration of NIH/3 T3 cells. TPP intervention significantly ameliorates oxidative stress and skin inflammation symptoms in UV-induced skin photoaging damage by restoring the activity of endogenous antioxidant enzymes and suppressing the mRNA expression of pro-inflammatory cytokines, including IL-1β , IL-6 , and TNF-α ., as well as inhibiting the MAPK/AP-1/MMP9 signaling pathways. This study proposes that TPP is a viable plant protein-derived nutritional supplement for the prevention of UV-induced skin photoaging damage.
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Current Research in Food Science
·
2025
·
IF 7
Xizhe Zhu,Liyue Yang,Zhiwen Ge,Wen Ouyang,Jinjin Wang,Ming Chen,Yaya Yu,Shunyin Wu,Yanhua Qin,Chunyong Huang,Guofu Zhang,Yating Zhang,Haibo Yuan,Yongwen Jiang,Jinjie Hua
Fixation methodology serves as the critical determinant in shaping green tea's multi-dimensional quality attributes; however, the impact of different fixation methods and the corresponding combinations on the overall metabolites and quality of in green tea remains unclear. In this study, non-volatile metabolites (NVMs) and volatile metabolites (VMs) analyses and objective quantitative techniques were used to determine the effects of electromagnetic roller-hot air coupling fixation (ROHF), electromagnetic roller-steam coupling fixation (ROSF), electromagnetic roller-hot air-steam coupling fixation (RHSF), electromagnetic single roller fixation (ROLF), and carding fixation (CDF) on the quality of green tea. Forty-four NVMs, 99 VMs, and 15 flavor objective quantitative indexes were identified, with 12 differential NVMs and 9 differential VMs statistically screened. The green tea processed via ROLF was lustrous and emerald green with a bright and clear liquor; ROSF resulted in low caffeine and flavonoid glycosides contents and an umami (UMS) taste; and RHSF was associated with high trans -β-ionone, hexanal and 1-octen-3-ol levels, with a floral and fresh aroma. These findings provide a precise control scheme for the customized processing of green tea, and promote the upgrading of traditional fixation process to intelligent standardization.
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Experimental and Therapeutic Medicine
·
2021
·Vol. 22
·期 6
·1-10
·
IF 2.447
Qian Li,Zhaoyan Qiu,Yan Wang,Chunyan Guo,Xu Cai,Yandong Zhang,Li Liu,Hongkun Xue,Jintian Tang
Tea polyphenols (TPs) are the major bioactive extract from green tea that have been extensively reported to prevent and treat oxidative stress damage. In previous studies, TPs have been demonstrated to protect cells against oxidative injury induced by hydrogen peroxide (H2O2). However, the underlying mechanism remains unclear. The aim of the current study was to investigate whether the protective and regulatory effects of TPs on oxidative stress damage were dependent on the mammalian STE20‑like protein kinase (Mst)/nuclear factor (erythroid‑derived 2)‑like 2 (Nrf2) axis and the Kelch‑like ECH‑associated protein 1 (Keap1)/Nrf2/heme oxygenase 1 (HO‑1) pathway in RAW264.7 cells, a murine macrophage cell line. Maintaining a certain range of intracellular reactive oxygen species (ROS) levels is critical to basic cellular activities, while excessive ROS generation can override the antioxidant capacity of the cell and result in oxidative stress damage. The inhibition of ROS generation offers an effective target for preventing oxidative damage. The results of the present study revealed that pretreatment with TPs inhibited the production of intracellular ROS and protected RAW264.7 cells from H2O2‑induced oxidative damage. TPs was also demonstrated to attenuate the production of nitric oxide and malondialdehyde and increase the levels of antioxidant enzymes (superoxide dismutase, catalase and glutathione peroxidase). In addition, following TPs treatment, alterations in Mst1/2 at the mRNA and protein level inhibited the production of ROS and promoted the self‑regulation of antioxidation. TPs‑induced Keap1 gene downregulation also increased the expression of Nrf2 and HO‑1. Collectively, the results of the present study demonstrated that TPs provided protection against H2O2‑induced oxidative injury in RAW264.7 cells.
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Journal of Functional Foods
·
2025
·
IF 4
Yan Ma,Zhengnan Jiang,Hai Xie,Jian Wang,Minni Li,Huifang Ge,Wenbin Li,Zhongwen Xie,Daxiang Li,Xueling Gao,Hongyan Wang
Fermented oxidation fresh tea (FOFT) is a unique dark tea produced by a single strain of Eurotium cristatum , and its impact on obesity has not been extensively studied. Here, we investigated the anti-obesity and metabolic benefits of FOFT using a HFD-induced obesity mouse model. The HFD-fed mice or ND-fed mice were orally administered with FOFT for 12 weeks. The results showed that FOFT invention significantly suppressed body weight gain, ameliorated liver and adipocyte morphology, and improved glucolipid metabolism and intestinal homeostasis imbalance in HFD-fed mice. Notably, FOFT administration caused no adverse effects in ND mice, indicating its safety at tested doses. Moreover, FOFT restored intestinal microbiome dysbiosis and elevated the short-chain fatty acid levels. This gut microbiome shift was associated with reduced systemic inflammation and attenuated hepatic lipid accumulation. These findings suggest that FOFT, as a novel functional food, holds great promise for resistance to obesity and its comorbidities.
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Aquaculture Reports
·
2022
·
IF 3.385
Yuru Zhang,Kedi Gao,Yanhua Ren,Junmei Zhang,Ronghua Lu,Xianglin Cao,Liping Yang,Xinxin Xu,Guoxing Nie
This study explored the protective effect of broken tea on growth, antioxidant capacity, nutritional composition and lipid metabolism in fish fed a high-fat (HF) diet. A total of 450 Cyprinus carpio (18.79 ± 3.16 g) were divided into five groups and fed for 8 weeks: the control diet group (C), HF diet group (HF), and the HF diet plus 1 %, 2 % and 3 % broken Xinyang Maojian tea (BT Maojian ) groups (HF+1%BT, HF+2 %BT and HF+3 %BT). We found that the weight gain rate (WGR) of carp was increased significantly in the HF+ 3 %BT group compared with the control group ( P < 0.05). The elevated hepatic malondialdehyde (MDA), alanine aminotransaminase (ALT), aspartate aminotransferase (AST), and triglyceride (TG) in the HF group were attenuated by BT Maojian treatment ( P < 0.05). Moreover, the low total antioxidant capacity (T-AOC) and superoxide dismutase (T-SOD) content in serum and hepatopancreas in the HF group were significantly increased by the addition of BT Maojian ( P < 0.05). The total essential amino acid content in muscle was significantly increased in the HF+ 2%BT group compared with the HF group ( P < 0.05). Additionally, the highest linoleic acid content in muscle was found in the HF+ 3%BT group. The excessive hepatopancreas fat accumulation caused by the HF diet was alleviated in the 2 % and 3 % BT Maojian supplementation groups. The mRNA level of most key lipid metabolism genes in the hepatopancreas, intestinal tract, muscle and adipose tissue tended to be increased in the HF group and decreased in the BT Maojian treatment groups. Taken together, these results indicated that the addition of 2 % or 3 % BT Maojian likely positively affected growth performance, enhanced flesh quality, and alleviated harmful symptoms, including low antioxidant capacity and excessive hepatic fat accumulation in carp fed a HF diet. Thus, BT Maojian can be used as a green feed additive in C. carpio feed.
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