MICROCHIMICA ACTA
·
2020
·Vol. 187
·期 6
·1-10
·
IF 6.232
Li Kun,Zhao Bao,Yu Qingcai,Xu Juan,Li Xuewei,Wei Diandian,Qian Lisheng,Liu Guodong,Wang Wenrui
A porous hydrophilic affinity bead consisting of graphene oxide and chitosan (pGC) with the honeycomb-biomimetic microchannels has been synthesized and applied as hydrophilic adsorbent for selective capture of glycopeptides. The pGC beads have open-porous structure, honeycomb-like microchannels, large interior voids, and hydrophilic property. Based on the multivalent hydrophilic interactions between glycan moieties on glycopeptides and amino groups and hydroxyl groups on chitosan, the glycopeptides were enriched and separated by pGC beads. The pGC beads exhibit high sensitivity (detection limit, 5 fmol), binding capacity (111.1 mg/g), enrichment selectivity (molar ratio of human IgG to BSA tryptic digests of 1:200), and recovery yield (89.78%). By combing pGC beads and nano LC-MS/MS analysis, a total of 325 N-glycosylated peptides corresponding to 152 N-glycosylated proteins were identified from 2 μL human serum. These experimental results demonstrate the practical application of the method in glycoproteomics research.
Schematic representation of fabrication for porous hydrophilic affinity beads (pGC) with honeycomb-biomimetic microchannels based on graphene oxide (GO) and chitosan (CS). The pGC was successfully applied to capturing and identifying low-abundant glycopeptides from biological samples.
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Scientific Reports
·
2020
·Vol. 10
·期 1
·1-9
·
IF 3.998
Li Zhenshun,Kuang Hairui,Yang Jinchu,Hu Jie,Ding Baomiao,Sun Weiqing,Luo Yangchao
Ovalbumin (OVA) is an important protein emulsifier. However, it is unstable near the isoelectric point pH, which limits its applications in the food industry. Polysaccharides may be explored to tackle this challenge by improving its pH-dependent instability. In this work, carboxymethyl cellulose (CMC) was used as a model polysaccharide to mix with OVA near its isoelectric point (pH 4.7) with subsequent mild heating at 60 °C for 30 min. The molecular interactions between OVA and CMC were comprehensively studied via a series of characterizations, including turbidity, zeta potential, intrinsic fluorescence, surface hydrophobicity, circular dichroism (CD) spectra and Fourier transform infrared spectroscopy (FTIR). The droplet sizes of the emulsions prepared by OVA-CMC were measured to analyze emulsifying property and stability. The results indicated that free OVA was easily aggregated due to loss of surface charges, while complexing with CMC significantly inhibited OVA aggregation before and after heating owing to the strong electrostatic repulsion. In addition, OVA exposed more hydrophobic clusters after heating, which resulted in the growth of surface hydrophobicity. Altogether, the heated OVA-CMC complexes presented the best emulsifying property and stability. Our study demonstrated that complexing OVA with CMC not only greatly improved its physicochemical properties but also significantly enhanced its functionality as a food-grade emulsifying agent, expanding its applications in the food industry, as development of emulsion-based acidic food products.
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CHEMICAL RESEARCH IN CHINESE UNIVERSITIES
·
2020
·Vol. 36
·期 5
·962-969
·
IF 1.063
Yu Wenting,Xue Bin,Zhu Zhenshu,Shen Ziqin,Qin Meng,Wang Wei,Cao Yi
Injectable hydrogels are ideal biomaterials for delivering cells, growth factors and drugs specifically to localized lesions and subsequent controlled release. Many factors can affect the efficacy of injectable hydrogels. To avoid potential damage to encapsulated cells or drugs, injectable hydrogels should be highly dynamic so that they can undergo shear-thinning at low strain rates and rapidly reform after injection. However, dynamic hydrogels are often mechanically weak, leading to the leakage of encapsulated cells or drugs. Here we demonstrated a convenient method to improve the mechanical strength without jeopardizing the dynamic properties of hydrogels by using metal ion-peptide crosslinkers containing multiple metal ion-ligand bonds. We showed that the dynamic properties of the hydrogels correlated with the intrinsic dynamics of the metal-ligand bonds and were not affected by the formation of multivalent binding. Yet, the mechanical stability of the hydrogels was significantly improved due to the increased thermodynamic stability of the crosslinkers. We further showed that the drug release rates were slowed down by the formation of multivalent crosslinkers. Our results highlight the importance of ligand valency to the mechanical response of hydrogels and provide a universal route to rationally tune the dynamic and mechanical properties of injectable hydrogels.
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NATURE MATERIALS
·
2020
·Vol. 19
·期 5
·528-533
·
IF 38.663
Zeng Mengqi,Liu Jinxin,Zhou Lu,Mendes Rafael G.,Dong Yongqi,Zhang Min-Ye,Cui Zhi-Hao,Cai Zhonghou,Zhang Zhan,Zhu Daming,Yang Tieying,Li Xiaolong,Wang Jianqiang,Zhao Liang,Chen Guoxian,Jiang Hong,Rümmeli Mark H.,Zhou Hua,Fu Lei
Developing a precise and reproducible bandgap tuning method that enables tailored design of materials is of crucial importance for optoelectronic devices. Towards this end, we report a sphere diameter engineering (SDE) technique to manipulate the bandgap of two-dimensional (2D) materials. A one-to-one correspondence with an ideal linear working curve is established between the bandgap of MoS 2 and the sphere diameter in a continuous range as large as 360 meV. Fully uniform bandgap tuning of all the as-grown MoS 2 crystals is realized due to the isotropic characteristic of the sphere. More intriguingly, both a decrease and an increase of the bandgap can be achieved by constructing a positive or negative curvature. By fusing individual spheres in the melted state, post-synthesis bandgap adjustment of the supported 2D materials can be realized. This SDE technique, showing good precision, uniformity and reproducibility with high efficiency, may further accelerate the potential applications of 2D materials.
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APPLIED PHYSICS A-MATERIALS SCIENCE & PROCESSING
·
2020
·Vol. 126
·期 3
·1-9
·
IF 1.81
Ding Feng,Lin Jing,Wu Tengyan,Zhong Hongbin
Magnetic nanoparticles were one of the most promising materials for targeted magnetic hyperthermia application due to the excellent magnetic properties. In this work, the magnetic nanoferrite of Mn 0.6 Zn 0.4 La x Fe 2− x O 4 ( x = 0.09, 0.1, 0.15, 0.2, 0.25 and 0.3) was synthesized by the hydrothermal method. Various physical properties of samples have been characterized by X-ray diffraction (XRD), Fourier transform infrared (FTIR), transmission electron microscopy and vibrating sample magnetometer. XRD results revealed that the lattice constants a t and crystallite size of the Mn–Zn nanoferrites decreased with increasing La 3+ content. The characteristic absorption bands of spinel nanoferrites and polyethylene glycol were observed in the FTIR spectra. FTIR results indicated that polyethylene glycol was coated on the Mn–Zn nanoferrites successfully. The saturation magnetization ( M S ), magnetic moment ( M r ) and anisotropy constant ( K ) were affected by La 3+ ion content. The M S of Mn 0.6 Zn 0.4 La 0.09 Fe 1.91 O 4 was 58 emu/g, which could be potential hyperthermia in biomedical application.
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POLYMER BULLETIN
·
2020
·Vol. 78
·期 6
·2993-3008
·
IF 2.014
Chen Yuying,Chen Juan,Li Jianping,Jiang Jingzhe,Fang Ziang,Lin Xing,Jiang Yan,Zhang Hongwen,Liu Hongbo
In this study, a macroinitiator of β-CD-ONa was prepared by a substitution reaction of β-cyclodextrin (β-CD) with sodium (Na). The macroinitiator was then used in the ring-opening polymerization of octamethylcyclotetrasiloxane (D 4 ) to obtain a star polymer, in which β-CD is the core and polysiloxane is the arm. FTIR, NMR, TGA, CA, etc., were used to characterize the structure and properties of star polymers. The results indicated that there were four polysiloxane arms attached to each β-CD core, and the star polymer had good thermal stability. With the increase in D 4 monomer dosage, the hydrophobicity of the star polymer slightly improved, but the reaction time had no significant effect on hydrophobicity. When the star polymer was coated onto polyethylene terephthalate membrane, the transmittance increased from 87.6% to 92.8%, and the haze did not change significantly. Furthermore, the surface of star polymer porous membranes was prepared by the static breath-figure method using n -hexane as solvent at 30 °C. The results showed uniform pore distribution and increased hydrophobicity.
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Journal of Oceanology and Limnology
·
2020
·Vol. 38
·期 5
·1517-1531
·
IF 0.617
Tian Jing,Chen Zhuoyuan,Jing Jiangping,Feng Chang,Sun Mengmeng,Li Weibing
The Cu 2 O/TiO 2 p-n heterojunction composite photoelectrodes were prepared by depositing Cu 2 O nanoparticles on the surface of TiO 2 nanotubes via anodic oxidation and constant current deposition. Field emission scanning electron microscopy (SEM) and high-resolution transmission electron microscopy (HRTEM) analyses showed that Cu 2 O nanoparticles not only deposited on the surface of TiO 2 nanotube array, but also on the wall of TiO 2 nanotubes. The Cu 2 O deposition amount could be adjusted by changing the deposition time. The photoelectrochemical cathodic protection (PECCP) performance of the prepared photoelectrodes for 316L stainless steel (SS) was tested under visible light. The constant current deposition time had a significant effect on the PECCP performance of Cu 2 O/TiO 2 -X photoelectrodes and Cu 2 O/TiO 2 -20 had the best PECCP performance for the coupled 316L SS. This was attributed to the appropriate amount and thickness of Cu 2 O to form p-n heterojunctions with TiO 2 , in which separation of the photogenerated carriers was accelerated and transfer of the photogenerated electrons to 316L SS for PECCP was facilitated.
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JOURNAL OF POLYMER RESEARCH
·
2020
·Vol. 27
·期 6
·1-5
·
IF 2.426
Wang Zhanwen,Li Shizhuo,Wang Jianhua,Han Enlin,Tian Guofeng,Wu Dezhen
Polyimide (PI)/ bisphenol A dicyanate esters (BADCy) composite laminates with varying resin contents were prepared by filament winding and autoclave molding process. The laminates exhibited ultralow dielectric constant ( ε of 3.15–3.25) and dielectric loss (tan δ of 0.004–0.006) as well as excellent mechanical properties. When the resin content was 35.6%, the composite achieved outstanding comprehensive properties with tensile strength and modulus equal to 1485.1 MPa and 80.9 GPa, respectively, and the inter-laminar shear strength (ILSS) up to 66.2 MPa. The failure mode of laminates followed an explosive gage middle (XGM) mechanism, which suggested a favorable interface between fiber and resin. According to the mixed law equation, PI fiber at a frequency of 10.2 GHz exhibited dielectric constant of 3.41 ± 0.03 and dielectric loss of about 0.002–0.004. The successful preparation of PI fiber reinforced BADCy composite with low dielectric constant provides new ideas for material design and selection of lightweight high-strength structural-functional integrated composites.
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CHINESE JOURNAL OF POLYMER SCIENCE
·
2020
·Vol. 38
·期 9
·983-992
·
IF 3.154
Yang Guang-Yao,Tong Li-Fen,Liu Xiao-Bo
Multi-walled carbon nanotubes (MWCNTs) modified with silane coupling agent A-1120 (MWCNTs-A1120) were prepared. Compared with the raw MWCNTs, acidified MWCNTs (MWCNTs-COOH), and MWCNTs grafted with EDA (MWCNTs-NH 2 ), MWCNTs-A1120 have the best dispersion in fluoroelastomer at the same doping ratio. Therefore, fluoroelastomer/MWCNTs-A1120 composite has the best mechanical properties with tensile strength of 13.92 MPa and elongation at break of 111.78%. Then, the effects of doping amount of MWCNTs-A1120 on the electrical properties of the composites were investigated. The dielectric constant of the composite increases with the increase of MWCNTs-A1120, and the dielectric loss does not change much at the low doping amount such as 0.5 wt%. When the doping amount of MWCNTs-A1120 is 5 wt%, the dielectric constant and the dielectric loss value are greatly increased, and the volume resistivity is greatly decreased, which proves that the conductive network is formed in the composite, so the filling amount of 5 wt% is the percolation threshold. The tensile deformation of the sample also affects the electrical properties of the composites. As the tensile deformation increases, the dielectric constant and dielectric loss of the composite decrease. For the composite with 5 wt% MWCNTs-A1120, excessive tensile deformation will destroy the conductive network structure of the composite, so the composite will change from conductive material to dielectric material. Therefore, such composite is a good candidate for flexible conductive material or flexible dielectric material used in harsh environments such as high temperatures and various aggressive solvents.
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CHEMICAL PAPERS
·
2020
·Vol. 74
·期 7
·2145-2152
·
IF 1.68
Zhang Xu,Ye Qingqing,Fan Yingtao,Hu Xiaoyu,Shen Yingzhong
Three novel phosphorescent host materials, 4′,4‴-(4-phenylpyridine-2,6-diyl)bis( N , N -diphenyl-[1,1′-biphenyl]-4-amine) (P1), 2,6-bis(4′-(naphthalen-1-yl)-[1,1′-biphenyl]-4-yl)-4-phenylpyridine (P2) , and 2,6-bis(3′,5′-dimethyl-[1,1′-biphenyl]-4-yl)-4-phenylpyridine (P3) , based on triphenylpyridine with a symmetrical molecular conformation were designed and synthesized from 2,6-bis(4-bromophenyl)-4-phenylpyridine (3) reacting with [4-(diphenylamino)phenyl]boronic acid (A1) , [4-(naphthalen-1-yl)phenyl]boronic acid (A2) , and (3,5-dimethylphenyl)boronic acid (A3) through Suzuki coupling reaction, respectively. The compounds obtained were characterized by elemental analysis, 1 H NMR, and 13 C NMR spectroscopy. The thermal properties of these materials were studied by thermogravimetric analysis and differential scanning calorimetry measurements, which showed that they exhibited excellent thermal stability with glass transition temperatures over 94 °C. Their photophysical properties were also investigated, and the results indicated that all compounds possessed considerably high triplet energy level greater than 2.6 eV; they also emitted blue fluorescence in different solvents. Finally, the results of the density function theory calculations revealed that the compound P1 showed the well-separated frontier molecular orbital, which indicated that it possessed bipolar carrier transport ability for holes and electrons, whereas the compound P2 and P3 exhibited greater overlap between the highest occupied molecular orbital and the lowest unoccupied molecular orbital.
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ONCOGENE
·
2020
·Vol. 39
·期 11
·2408-2423
·
IF 7.971
Guan Yun-feng,Huang Qiao-ling,Ai Yuan-li,Chen Qi-tao,Zhao Wen-xiu,Wang Xiao-min,Wu Qiao,Chen Hang-zi
Hepatocellular carcinoma (HCC) is one of the leading causes of cancer-related mortality worldwide. Orphan nuclear receptor Nur77, which is low expressed in HCC, functions as a tumor suppressor to suppress HCC. However, the detailed mechanism is still not well understood. Here, we demonstrate that Nur77 could inhibit HCC development via transcriptional activation of the lncRNA WAP four-disulfide core domain 21 pseudogene (WFDC21P). Nur77 binds to its response elements on the WFDC21P promoter to directly induce WFDC21P transcription, which inhibits HCC cell proliferation, tumor growth, and tumor metastasis both in vitro and in vivo. In clinical HCC samples, WFDC21P expression positively correlated with that of Nur77, and the loss of WFDC21P is associated with worse prognosis. Mechanistically, WFDC21P could inhibit glycolysis by simultaneously interacting with PFKP and PKM2, two key enzymes in glycolysis. These interactions not only abrogate the tetramer formation of PFKP to impede its catalytic activity but also prevent the nuclear translocation of PKM2 to suppress its function as a transcriptional coactivator. Cytosporone-B (Csn-B), an agonist for Nur77, could stimulate WFDC21P expression and suppress HCC in a WFDC21P-dependent manner. Therefore, our study reveals a new HCC suppressor and connects the glycolytic remodeling of HCC with the Nur77-WFDC21P-PFKP/PKM2 axis.
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BMC Chemistry
·
2020
·Vol. 14
·期 1
·1-7
·
IF 0
Ye Changwen,He Chen,Zhang Bowen,Wang Lixuan,Wang Lufeng
Zizania latifolia is easily lignified after harvesting, leading to the degradation of food quality and commercial value. Thus, this study evaluated the effect of radio frequency (RF) treatments on lignification inhibition of Zizania latifolia . The results showed that the lignin content of Zizania latifolia treated with RF decreased significantly compared with the control group. At the 7th day of storage, the phenylalanine ammonia lyase activity of the 90 W RF treatment group decreased by 52.9% compared with the initial value. The activities of peroxidase and polyphenol oxidase in the stems of Zizania latifolia were significantly ( p < 0.05) decreased after RF treatments. Besides, a decrease in conversion rate of O 2 − and H 2 O 2 to downstream products was observed, indicating that the related invertases were inhibited by RF treatment. All of these showed that RF treatments contribute to inhibit or delay the lignification of Zizania latifolia , providing a better taste and quality for products.
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