Molecular Medicine Reports
·
2014
·Vol. 11
·期 4
·2413-2420
·
IF 1.484
Jing Ma,Ling Xi Xing,Ming Shen,Fan Li,Ming Jie Zhu,Li Fang Jin,Zhaojun Li,Feng Gao,Yijin Su,You Rong Duan,Lian Fang Du
A combination of diagnostic and therapeutic ultrasound (US) techniques may be able to provide the basis of specific therapeutic protocols, particularly for the treatment of tumors. Nanotechnology may aid the progression towards the use of US for tumor diagnosis and targeted therapy. The current study investigated in vivo and in vitro US contrast imaging using nanocapsules (NCs), and also US and US‑targeted microbubble destruction (UTMD) therapy using drug‑loaded NCs for pancreatic cancer in vitro. In the current study, the NCs were made from the polymer nanomaterial poly(lactic‑co‑glycolic acid)‑monomethoxypoly(ethylene glycol) (PLGA‑mPEG), encapsulated with paclitaxel (PTX), to create PTX‑PLGA‑mPEG NCs. The PTX‑PLGA‑mPEG NCs were used as a US contrast agent (UCA), which produced satisfactory US contrast‑enhanced images in vitro and in vivo of the rabbit kidneys, with good contrast compared with lesions in the peripheral regions. However, clear contrast‑enhanced images were not obtained using PTX‑PLGA‑mPEG NCs as a UCA, when imaging the superficial pancreatic tumors of nude mice in vivo. Subsequently, fluorescence and flow cytometry were used to measure the NC uptake rate of pancreatic tumor cells under various US or UTMD conditions. An MTT assay was used to evaluate the efficiency of PTX and PTX‑PLGA‑mPEG NCs in killing tumor cells following 24 or 48 h of US or UTMD therapy, compared with controls. The specific US or UTMD conditions had been previously demonstrated to be optimal through repeated testing, to determine the conditions by which cells were not impaired and the efficiency of uptake of nanoparticles was highest. The current study demonstrated high cellular uptake rates of PLGA‑mPEG NCs and high tumor cell mortality with PTX‑PLGA‑mPEG NCs under US or UTMD optimal conditions. It was concluded that the use of NCs in US‑mediated imaging and antitumor therapy may provide a novel application for US.
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Nanoscale
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2015
·Vol. 8
·期 5
·3100-3118
·
IF 7.394
Di Jiang,Xiaoling Gao,Ting Kang,Xingye Feng,Jianhui Yao,Mengshi Yang,Yixian Jing,Qianqian Zhu,Jingxian Feng,Jun Chen
Drug resistance is the major reason for therapeutic failure during cancer treatment. Chemo-photodynamic combination therapy has potential to improve the treatment efficiency in drug-resistant cancers, but is limited by the incompatible physical properties of the photosensitizer with a chemo-drug and poor accumulation of both drugs into the inner areas of the tumor. Herein, a novel drug delivery system was designed by incorporating the photosensitizer, chlorine 6, chemically in the shell and the chemo-drug, doxorubicin, physically in the core of D-α-tocopheryl polyethylene glycol 1000 succinate-poly(lactic acid) (TPGS-PLA) nanoparticles with a targeting ligand, tLyp-1 peptide, decorated over the surface (tLyp-1-NP). This nanoparticle with a high drug loading capacity of both the photosensitizer and chemo-drug is expected to realize chemo-photodynamic combination therapy of drug-resistant cancer and simultaneously achieve the specific deep penetration and accumulation of drugs into the inner areas of tumor. tLyp-1-NP was prepared via a nanoprecipitation method and it exhibited a uniformly spherical morphology with a size of approximately 130 nm. After appropriate irradiation, tLyp-1-NP showed high cellular uptake and strong cytotoxicity in both human umbilical vein endothelial cells (HUVEC cells) and doxorubicin-resistant human breast adenocarcinoma cells (MCF-7/ADR cells) in vitro. After intravenous administration, compared with the unmodified NPs, tLyp-1-NP was found to have superior tumor targeting ability and more potent reversion of doxorubicin-resistant cancer. The terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling and the hematoxylin and eosin staining of the treated tumors further demonstrated the anti-tumor efficacy of tLyp-1-NP in the presence of a laser. These observations collectively suggest the potential of tLyp-1-NP for the actively targeting chemo-photodynamic combination therapy of drug-resistant cancer.
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COLLOIDS AND SURFACES B-BIOINTERFACES
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2025
·
IF 5.6
Hao Zhou,Zhiwen Qi,Hongxia Chen,Xingying Xue,Lingyan Liu,Chengzhang Wang
In recent years, various antibiotic-resistant bacterial species have emerged, thereby complicating bacterial infection treatment. Hence, the development of nontraditional, multifunctional, cationic photodynamic antibacterial agents is imperative and valuable to inhibit normal and multidrug-resistant bacterial strains. Here, by performing ring-opening reactions, we successfully synthesized an antibacterial polycation (EY-QEGDM-MG) based on gallic acid, ethylene glycol diglycidyl ether, and eosin Y and enriched with various functional components, namely a photosensitizer, quaternary ammonium (QA), and hydroxyl species. Under light irradiation, the polymer inhibited the growth of both Escherichia coli and methicillin-resistant Staphylococcus aureus because of the combined antibacterial activities of the photosensitizer and the QA group. Evaluation of the mechanism of the antibacterial effect revealed that the polymer binds to the bacterial cell surface and irreversibly damages the cell wall and membrane structure. Furthermore, the polymer also exhibited low cytotoxicity and good hemocompatibility. The antibacterial efficacy of the polymer was assessed in vivo on a rat wound infection model; the polymer showed sustainably enhanced therapeutic effects. Alterations in the levels of CD31-expressing cells and CD68 + macrophages and results of histological analyses validated that the polymer could inhibit bacterial infection in the mice model. The present study provides an efficient approach to develop an effective antimicrobial agent to reduce hospital-acquired bacterial infections.
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EUROPEAN POLYMER JOURNAL
·
2026
·
IF 6.8
Nutcha Nongyai,Jakkree Intranuwong,Seong Kyu Shin,Mooheon Kim,Yong Ku Kwon
Amphiphilic diblock copolymers of poly( γ -phenyl- ε -caprolactone)- block -methoxy poly(ethylene glycol) (PPhCL x - b -mPEG, where x represents the PhCL mol% in the copolymer) were synthesized via cationic ring-opening polymerization (CROP) and proceeded through an activated monomer mechanism (AMM), with variation of the hydrophobic block length. The introduction of phenyl substituents retarded the polymerization rate and disrupted chain packing, leading to reduced crystallinity compared to analogous poly( ε -caprolactone)- block -mPEG (PCL n - b -mPEG where n represents the PCL mol% in the copolymer) at comparable molecular weight. The micellization behavior of PPhCL x - b -mPEGs was investigated to evaluate their emulsification capability. Despite greater hydrophobicity, as evidenced by higher water contact angles, PPhCL x - b -mPEG exhibited a significantly higher critical micelle concentration (CMC), which is attributed to steric hindrance during micelle core formation. Emulsion stability measurements revealed that high internal phase emulsions (HIPEs) stabilized by PPhCL x - b -mPEGs exhibited significantly improved stability compared with those stabilized by PCL 67 - b -mPEG. PPhCL x - b -mPEGs were successfully employed as emulsifiers for HIPE polymerization, resulting in the formation of highly porous polyHIPE materials. The observed decrease in pore size with increasing PPhCL chain length demonstrated the improved structure-directing capability of PPhCL x - b -mPEG in the formation of polyHIPE foams. Compared to conventional small-molecule surfactants, this approach enables precise control over pore morphology through systematic variation of block length during synthesis, representing a versatile strategy for tailoring polyHIPE structures.
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SOLAR ENERGY MATERIALS AND SOLAR CELLS
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2023
·
IF 6.9
Yifan Jia,Guoxing Liao,Yang Wu,Oleksandr Mykhaylyk,Paul D. Topham,Xue-Hui Dong,Changzhong Chen,Qianqian Yu,LinGe Wang
Form-stable phase change fibers (PCFs) that are composed of stable supporting material (as polymer matrix) and phase change material (PCM, as working ingredient) have become novel smart materials, being widely applied in energy storage, thermal regulation and the biomedical field . However, the inherent limitation of PCFs is the restriction of the supporting material on the crystallization of the PCM, which results in low phase change enthalpy (Δ H ). Here, six different types of PCF comprised of poly(lactic acid) (PLA, as supporting material) and poly(ethylene glycol) (PEG, as PCM) have been fabricated. Their morphology, phase change performance and structural transitions during phase change have been extensively studied. We reveal that the crystallizability and glass transition temperature of PLA have a dominating influence on Δ H . In the optimum system, PLA/PEG PCFs can achieve up to 104 J/g Δ H which is close to the theoretical value of pristine PEG. This high-performance PCF also exhibits only 2.5% Δ H loss after 100 thermal cycles and good ability in thermal energy storage and thermal regulation. This work provides a promising form-stable PCF and introduces a new strategy towards developing high Δ H PCFs by selecting suitable supporting materials.
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TALANTA
·
2023
·
IF 6.1
Simin Chen,Yiqi Qie,Zhendong Hua,Haoyue Zhang,Youmei Wang,Bin Di,Mengxiang Su
Phytocannabinoids and their synthetic analogs (natural and synthetic cannabinoids) are illicit drugs that are widely abused worldwide. Wastewater-based epidemiology (WBE) is an objective approach for the estimation of population-level exposure to a wide range of substances, especially drugs of abuse. However, the concentrations of cannabinoids in wastewater are extremely low (frequently at the levels of nanograms per liter), and the existing pretreatment procedures for wastewater have the disadvantages of time-consumption or low extraction recoveries. This study aimed to propose a novel poly (methacrylic acid- co -ethylene glycol dimethacrylate)-functionalized polydopamine-coated Fe 3 O 4 nanoparticle (Fe 3 O 4 @ [email protected] (MAA- co -EGDMA)) as an adsorbent, and provide a highly sensitive quantitative analytical technique for the detection of five synthetic cannabinoids (SCs: 5 F-EDMB-PINACA, FUB-APINACA, MDMB-4en-PINACA, MDMB-FUBINACA, and PB-22) and one cannabis-related human metabolite (THC-COOH) in wastewater. The magnetic adsorbents were fully characterized by transmission electron microscopy (TEM), infrared spectroscopy (IR), vibrating sample magnetometry (VSM) and X-ray photoelectron spectroscopy (XPS). Subsequently, an MSPE-UHPLC-MS/MS method was developed and validated for the determination of six trace analytes in wastewater. The validation results showed that the method has limits of quantification as low as 0.1–1.0 ng/L. Additionally, the recoveries ranged from 62.81 to 124.02%, and the relative standard deviations (RSDs) of intraday and interday precision were less than 15%. This MSPE-UHPLC-MS/MS method was successfully applied to real wastewater samples, and the whole analytical process of one sample from pretreatment to the obtained quantitative results was completed in less than 30 min. Thus, the proposed method based on Fe 3 O 4 @ [email protected] (MAA- co -EGDMA) is a convenient, rapid, sensitive and reliable method for the determination of trace psychoactive drugs in wastewater.
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PHARMACEUTICAL RESEARCH
·
2021
·Vol. 38
·期 12
·2091-2108
·
IF 4.2
Ding Ning,Zhao Zhiqing,Yin Na,Xu Ying,Yin Tian,Gou Jingxin,He Haibing,Wang Yanjiao,Zhang Yu,Tang Xing
Purpose The intention of the study was to co-delivery gemcitabine and cisplatin with totally different nature by prodrug and micelle strategy to improve its in vivo stability and antitumor effect. Methods A prodrug of gemcitabine (mPEG-PLG-GEM) was synthesized through the covalent conjugation between the primary amino group of gemcitabine and the carboxylic group of poly (L-glutamic acid)-g-methoxy poly (ethylene glycol) (mPEG-PLG). It was prepared into micelles by a solvent diffusion method, and then combined with cisplatin through chelation to prepare gemcitabine and cisplatin co-loaded mPEG-PLG micelles (mPEG-PLG-GEM@CDDP micelles) . Results Gemcitabine and cisplatin in each micelle group were released more slowly than in solutions. In addition, pharmacokinetics behaviors of them were improved after encapsulated in prodrug micelles. T 1/2z of gemcitabine and cisplatin encapsulated in micelles were prolonged to 6.357 h (mPEG-PLG-GEM), 10.490 h (mPEG-PLG@CDDP), 5.463 h and 12.540 h (mPEG-PLG-GEM@CDDP) compared with GEM@CDDP solutions (T 1/2z = 1.445 h and 7.740 h). The ratio of synergy between gemcitabine and cisplatin (3:1 ~ 1:1(n/n)) was guaranteed in the systemic circulation, thus improving its antitumor effect. The results of biochemical analysis showed that GEM@CDDP-Sol was more toxic to kidneys and marrow compared with mPEG-PLG-GEM@CDDP micelles. Conclusions By prodrug strategy, gemcitabine and cisplatin with totally different nature were prepared into micelles and obtained a better pharmacokinetic behavior. And the dual drug delivery system performed a better in vivo stability and antitumor effect compared with each single drug delivery system in the experiment. Graphical abstract
Scheme. Schematic of mPEG-PLG-GEM@CDDP micelles’ formation and action process.
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JOURNAL OF ORGANOMETALLIC CHEMISTRY
·
2021
·
IF 2.369
Xueyi Chang,Qun Luo,Yongfu Qiu,Zhiyu Cheng,Guiping Tan,Tao Guan,Biye Ren
ABSTRACT In order to apply reversible self- and dis-assembly processes of polymers controlled by electrochemical redox to drug delivery systems, two novel polymeric ferrocenyl amphiphiles, 4-(11-Ferrocenylundecyloxy) benzoic acid methoxy poly(ethylene glycol) ester (denoted as PEG-Ph-Fc) and (11-ferroceny -lundecyloxy) poly(ethylene glycol) methyl ether (denoted as PEG-Fc) were synthesized and their electrochemical redox-controlled self-assembly behaviors in aqueous solution were investigated by potentiostatic method and observed by Transmission Electron Microscopy (TEM) and Dynamic Light Scattering (DLS). It is observed that polymeric amphiphile PEG-Fc and PEG-Ph-Fc could reversibly aggregate to form spherical micelles and also disassemble into irregular fragments by electrochemical redox reaction in aqueous solution without adding other chemical reagents. This work offers significant possibilities for the fundamental science of controlled self-assembly and drug release.
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JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
·
2023
·
IF 3.4
Meiyun Shi,Xinyue Zheng,Yuncheng Ge,Ning Zhang,Luyao Yu,Xujian Duan,Yajun Liu,Hongyu Xue,Jiansong You,Lei Yin
Unraveling the cytotoxicity and cellular uptake of low, medium and high molecular weight polyethylene glycol (PEG) in cells is important for evaluation of therapeutic efficacy and toxicity of PEGylated drug delivery systems . In this study, cellular uptake of PEG600, PEG2K, PEG4K and PEG10K in MCF-7 cells was first studied by an UPLC-MS/MS assay coupled with collision induced dissociation (CID) in source technique. The CID of PEG in source with high values of declustering potentials generates numerous PEG-related product ions. These PEG-related fragment ions can be further broken into specific product ions in the collision cell as alternative ions for detection of PEG. The quantification of PEG was finally performed with the MRM transition ( m/z 221.0 → 89.0). The experimental results indicated that the toxicity of PEG600, PEG2K, PEG4K and PEG10K was not significant at concentrations of 5–1200 μg/mL and the amounts of PEG polymers entry into MCF-7 cells at was small. The greenness of the developed analytical methods was also assessed by Analytical Eco-Scale, Analytical Greenness calculator (AGREE) and Green Analytical Procedure Index (GAPI) in this study.
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Materials Science & Engineering C-Materials for Biological Applications
·
2020
·
IF 5.88
Naizhen Zhou,Xiaoe Ma,Wanjun Hu,Pengfei Ren,Youliang Zhao,Tianzhu Zhang
The extracellular matrix (ECM) affects cell behaviors, such as survival, proliferation, motility, invasion, and differentiation. The arginine-glycine-aspartic acid (RGD) sequence is present in several ECM proteins, such as fibronectin, collagen type I, fibrinogen, laminin, vitronectin, and osteopontin. It is very critical to develop ECM-like substrates with well-controlled features for the investigation of influence of RGD on the behavior of tumor cells. In this study, poly(ethylene glycol) (PEG)-crosslinked poly(methyl vinyl ether- alt -maleic acid) (P(MVE- alt -MA)) hydrogels (PEMM) with different RGD contents were synthesized, fully characterized, and established as in vitro culture platforms to investigate the effects of RGD content on cancer stem cell (CSC) enrichment. The morphology, proliferation, and viability of SK-OV-3 ovarian cancer cells cultured on hydrogels with different RGD contents, the expression of CSC markers and malignant signaling pathway-related genes, and drug resistance were systematically evaluated. The cell aggregates formed on the hydrogel surface with a lower RGD content acquired certain CSC-like properties, thus drug resistance was enhanced. In contrast, the drug sensitivity of cells on the higher RGD content surface increased because of less CSC-like properties. However, the presence of RGD in the stiff hydrogels (PEMM2) had less effect on the stemness expression than did its presence in the soft hydrogels (PEMM1). The results suggest that RGD content and matrix stiffness can lead to synergetic effects on the expression of cancer cell stemness and the epithelial-mesenchymal transition (EMT), interleukin-6 (IL-6), and Wnt pathways.
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JOURNAL OF CHROMATOGRAPHY A
·
2020
·
IF 4.049
Yongrui He,Meiling Qi
This work presents a new triptycene-based stationary phase (TP-PEG) combining the three-dimensional (3D) triptycene (TP) framework with polyethylene glycol (PEG) moieties for gas chromatographic (GC) separations. Its statically coated capillary column showed high column efficiency of 5263 plates/m determined by naphthalene at 120 °C. Its Rohrschneider-McReynolds constants and Abraham solvation system constants were measured to characterize its polarity and molecular interactions with analytes of different types. As evidenced, the TP-PEG column showed high-resolution performance for the isomers of anilines, phenols, halobenzenes and alkanes with distinct advantages over the PEG columns, particularly those critical isomers such as 3,5-/2,3-xylidine ( R = 2.94), m -/ p -chlorotoluene ( R = 1.92), p -/ m -cresol ( R = 1.89), 2,2-dimethylbutane/2-methylpentane ( R = 1.51), 2,2,3-trimethylbutane /2,3-dimethyl pentane ( R = 1.74) and 2,3-dimethylpentane/ n -heptane ( R = 1.92). In addition, it exhibited good column repeatability and reproducibility with the relative standard deviation (RSD) values of 0.02%–0.09% for run-to-run, 0.13%–0.22% for day-to-day and 2.7%–4.1% for column-to-column, respectively, and a wide operational temperature range (30 °C–280 °C) . Its application to GC–MS analysis of the essential oil of Osmanthus fragrans has proven its good potential for practical analysis of complex samples.
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MOLECULES
·
2019
·Vol. 25
·期 1
·47
·
IF 3.06
Chunlei Jiao,Xiao Tan,Aijun Lin,Wenjie Yang
Nanometer zero-valent iron (nZVI) has been widely used in the treatment of heavy metals such as hexavalent chromium (Cr(VI)). A novel composite of bead string-structured nZVI on modified activated carbon (nZVI–MAC) is prepared here, using polyethylene glycol as the stable dispersant rather than traditional ethanol during the loading process. The microstructure characterization shows that nZVI particles are loaded on MAC with a bead string structure in large quantity and stably due to the addition of hydroxyl functional groups on the surface by polyethylene glycol. Experiments on the treatment of Cr(VI) in wastewater show that the reaction process requires only 20 min to achieve equilibrium. The removal rate of Cr(VI) with a low concentration (80–100 mg/L) is over 99% and the maximum saturation removal capacity is up to 66 mg/g. The system converts Cr(VI) to trivalent chromium (Cr(III)) through an oxidation-reduction effect and forms an insoluble material with iron ions by coprecipitation, which is then adsorbed on the surface of the nZVI–MAC. The process conforms to the quasi-second order adsorption kinetics equation (mainly chemical adsorption process).Keywords:bead string structure;nZVI;polyethylene glycol;Cr(VI) wastewater
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