Foods
·
2022
·Vol. 11
·期 23
·3793
·
IF 5.561
Yang Liu,Jiali Lin,Tiantian Cheng,Yangjie Liu,Fuliang Han
Anthocyanins are substances with multiple physiological activities widely present in red wine, but the influence of structure (methylation, hydroxylation, acylation, glycosylation) on the transport remains ill-defined. In the present study, Caco-2 monolayers were used as an in vitro model of the absorptive intestinal epithelium to transport different types of anthocyanin samples. Results showed that both methylation and acetylation promote the level of transport. Monoglycoside standard exhibited higher transport amount and rate compared to diglycoside standard while the transport level of the monoglycoside mixture was unexpectedly lower than that of the diglycoside mixture. Caco-2 monolayers appeared to be more capable of transporting the single standard than the mixed standard. Meanwhile, the transport of anthocyanins in Caco-2 cell model showed time- and concentration-dependent trends. Anthocyanin treatment had a greater effect on sodium-dependent glucose transporter 1 (SGLT1) mRNA expression than glucose transporter 2 (GLUT2), and significantly down-regulated the protein expression of SGLT1. Although the low bioavailability of anthocyanins requires much more research, further evidence of the role of structure is provided.Keywords:wine;anthocyanins;Caco-2 cell monolayer;absorption;SGLT1;GLUT2
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POLYMER
·
2023
·
IF 4.6
Jiani Gao,Yilong Chu,Yuyang Yan,Shuangshuang Zhou,Xiaowei Li,Cheng-Bo Li
Here, poly(1,3,5-tri(thiophene-2-yl)benzene) (PTTB) membrane electrodes with porous surface structures were prepared by using an electropolymerization method. Firstly, a series of PTTB film electrodes with different morphologies are obtained, including open nanotubes , stacked flocs, dispersed cauliflower-like, and aggregated spheres, by separately depositing the PTTB films on different conductive substrates (GCE, silicon wafers, ITO , FTO). Secondly, this study explored the factors affecting the photoelectric properties and stability of PTTB film electrodes by varying the solvent, electrochemical method, scan rate, and number of scan cycles. Finally, the CV method and DCM/ACN mixed solvent were used in this experiment, and the best photoelectric effect of 3.2 μA was obtained under 3 scan cycles. In this study, the factors influencing the properties of PTTB by electrochemical polymerization methods are obtained through the exploration of PTTB film electrodes, providing a reference value for further combining PTTB films with other materials to prepare composite photoelectrodes .
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Gut Microbes
·
2023
·
IF 12.2
Jie Hu,Yifan Wu,Xingjian Zhou,Luyuan Kang,Shiyi Zhang,Yisi Liu,Yu Pi,Xilong Li,Junjun Wang,Dandan Han
The pathogenicity of Escherichia coli (E. coli) O157:H7 is predominantly associated with Shiga toxin 2 (Stx2) that poses a huge threat to human and animal intestinal health. Production of Stx2 requires expression of stx2 gene, which is located in the genome of lambdoid Stx2 prophage. Growing evidence has implicated that many commonly consumed foods participate in the regulation of prophage induction. In this study, we aimed to explore whether specific dietary functional sugars could inhibit Stx2 prophage induction in E. coli O157:H7, thereby preventing Stx2 production and promoting intestinal health. We demonstrated that Stx2 prophage induction in E. coli O157:H7 was strongly inhibited by L-arabinose both in vitro and in a mouse model. Mechanistically, L-arabinose at doses of 9, 12, or 15 mM diminished RecA protein levels, a master mediator of the SOS response, contributing to reduced Stx2-converting phage induction. L-Arabinose inhibited quorum sensing and oxidative stress response, which are known as positive regulators of the SOS response and subsequent Stx2 phage production. Furthermore, L-arabinose impaired E. coli O157:H7 arginine transport and metabolism that were involved in producing Stx2 phage. Collectively, our results suggest that L-arabinose may be exploited as a novel Stx2 prophage induction inhibitor against E. coli O157:H7 infection.
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CERAMICS INTERNATIONAL
·
2023
·
IF 5.2
Xuan Wang,Zhenkuang Lei,Chunlin Zheng,Xueheng Zhuang,Qikui Man,Guoguo Tan
Semiconductor metal oxides are widely used in the field of wave absorption because of their easily tunable dielectric properties . Doping or modulating the morphology has been demonstrated to be an effective method to improve the microwave (MW) absorbing performance of semiconductor metal oxides. In this study, Ce-doped SnO 2 was prepared by a simple and effective coprecipitation method. Single-crystal SnO 2 nanorods were generated during the doping process and the size of the nanorods could be controlled by modifying the concentration of Ce. The presence of nanorods greatly enhances the MW absorbing performance: when the doping molar ratio is 3%, the minimum reflection loss of the absorber is −77.91 dB at a frequency of 13.79 GHz with a thickness of 1.6 mm and the maximum effective absorbing bandwidth is 4.47 GHz at a thickness of 1.5 mm. Excellent MW absorbing performance of Ce-doped SnO 2 is attributed to its excellent impedance matching and interfacial polarization.
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CELLULAR AND MOLECULAR LIFE SCIENCES
·
2023
·Vol. 80
·期 8
·1-18
·
IF 8
Cao Xuwen,Xie Yusu,Yang Hanwen,Sun Peiqi,Xue Beining,Garcia L. Rene,Zhang Liusuo
Dietary intake and nutrient composition regulate animal growth and development; however, the underlying mechanisms remain elusive. Our previous study has shown that either the mammalian deafness homolog gene tmc-1 or its downstream acetylcholine receptor gene eat-2 attenuates Caenorhabditis elegans development in a chemically defined food CeMM ( C. elegans maintenance medium) environment, but the underpinning mechanisms are not well-understood. Here, we found that, in CeMM food environment, for both eat-2 and tmc-1 fast-growing mutants, several fatty acid synthesis and elongation genes were highly expressed, while many fatty acid β-oxidation genes were repressed. Accordingly, dietary supplementation of individual fatty acids, such as monomethyl branch chain fatty acid C17ISO, palmitic acid and stearic acid significantly promoted wild-type animal development on CeMM, and mutations in either C17ISO synthesis gene elo-5 or elo-6 slowed the rapid growth of eat-2 mutant. Tissue-specific rescue experiments showed that elo-6 promoted animal development mainly in the intestine. Furthermore, transcriptome and metabolome analyses revealed that elo-6 /C17ISO regulation of C. elegans development may be correlated with up-regulating expression of cuticle synthetic and hedgehog signaling genes, as well as promoting biosynthesis of amino acids, amino acid derivatives and vitamins. Correspondingly, we found that amino acid derivative S-adenosylmethionine and its upstream metabolite methionine sulfoxide significantly promoted C. elegans development on CeMM. This study demonstrated that C17ISO, palmitic acid, stearic acid, S-adenosylmethionine and methionine sulfoxide inhibited or bypassed the TMC-1 and EAT-2-mediated attenuation of development via metabolic remodeling, and allowed the animals to adapt to the new nutritional niche.
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BIOPROCESS AND BIOSYSTEMS ENGINEERING
·
2023
·
IF 3.8
Jia Dong-Xu,Yu Hai,Wang Fan,Jin Li-Qun,Liu Zhi-Qiang,Zheng Yu-Guo
Cellobiose 2-epimerase (CE) is ideally suited to synthesize lactulose from lactose, but the poor thermostability and catalytic efficiency restrict enzymatic application. Herein, a non-characterized CE originating from Caldicellulosiruptor morganii (CmCE) was discovered in the NCBI database. Then, a smart mutation library was constructed based on FoldX ΔΔG calculation and modeling structure analysis, from which a positive mutant D226G located within the α 8 /α 9 loop exhibited longer half-lives at 65–75 °C as well as lower K m and higher k cat / K m values compared with CmCE. Molecular modeling demonstrated that the improvement of D226G was largely attributed to the rigidification of the flexible loop, the compactness of the catalysis pocket and the increment of substrate-binding capability. Finally, the yield of synthesizing lactulose catalyzed by D226G reached 45.5%, higher than the 35.9% achieved with CmCE. The disclosed effect of the flexible loop on enzymatic stability and catalysis provides insight to redesign efficient CEs to biosynthesize lactulose. Graphical abstract
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ACS Applied Materials & Interfaces
·
2023
·
IF 9.5
Shuang Yang,Yinan Lao,Wenjin Yu,Zhenyu Tang,Haoqing Guo,Ganghong Liu,Bo Qu,Lixin Xiao,Zhijian Chen
Tin-based perovskites comprise one of the preferred nontoxic alternatives to Pb-based perovskites due to their desirable optoelectronic properties. However, there remains a crucial stability problem due to the property of Sn2+ oxidation. In this study, we reported stable tin-based perovskite nanocrystals (NCs) using stannous acetate as the Sn2+ source because of its stronger Sn–O bonding. To prevent the oxidation of Sn2+, a thin layer of CsBr coverage was formed in situ; tin-based perovskite NCs, CsxSnBrx+2@CsBr (1 < x < 4), show a high photoluminescence quantum yield (PLQY) of 78.2% and high stability. The measured lifetime of PLQY decrease to half of the initial value is ∼1287 h under ambient conditions and ∼2200 h under a nitrogen atmosphere, respectively. Furthermore, the as-fabricated light-emitting diodes based on CsxSnBrx+2@CsBr NCs as the emitting layer exhibit a maximum luminescence of 16 cd/m2 and an external quantum efficiency of 0.035% with peaks at 451 and 615 nm, corresponding to the emissions of CsBr and CsxSnBrx+2, respectively. This work provided a new way to obtain stable Sn-based perovskite NCs and exhibited their potential for application in white light-emitting diodes (LEDs).
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ANALYST
·
2023
·Vol. 148
·期 15
·3603-3609
·
IF 4.2
Ju-Zheng Wang,Yi-Xuan Li,Qiaoting Yang,Junji Li,Jérome Chauvin,Xue-Ji Zhang,Serge Cosnier,Robert S. Marks,Dan Shan
The emergence of novel pathogens, as well as their frequent variants, raises the significance of developing superior and versatile sensing materials and techniques. Herein, a post-modified zeolitic imidazolate framework (pm-ZIF) was synthesized by using ZIF-67 as a parent MOF, and zinc(II) meso-tetra (4-carboxyphenyl) porphine (ZnTCPP) as a successive exchange ligand. Due to the preservation of the tetrahedral Co–N4 units from the ZIF precursor and the introduced porphyrin luminophores, this hybrid material pm-ZIF/P(Zn) enables the linear electrochemiluminescence (ECL) signal conversion of the target DNA concentration. An efficient biosensor that can be used to quantitatively detect SARS-CoV-2 was therefore constructed. The linear range of the sensor was 10−12–10−8 M, with a limit of detection (LOD) reaching 158 pM. Compared with the traditional amplification-based methods, the duration time of our method is significantly shortened and the quantitation of the SARS-Cov-2 RdRp gene can be completed within twenty minutes at room temperature.
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Inorganic Chemistry Frontiers
·
2023
·Vol. 10
·期 15
·4496-4502
·
IF 7
Lei Wu,Ruixin Zhang,Qun Jing,Hongyu Huang,Xianmeng He,Zhongchang Wang,Zhaohui Chen
As nonlinear optical (NLO) materials, phosphates often suffer from weak second harmonic generation (SHG) response and low birefringence. Here, we report the successful synthesis of a new UV NLO orthophosphate, β-Li2RbBi(PO4)2, by the high temperature solution method, and demonstrate that it crystallizes in a polar space group of P21 and is composed of 1D 1[Bi4(PO4)8]∞ infinite chains that are constructed from 1[Bi2O11]∞ chains and [PO4] clusters. The optical functional motifs 1[Bi4(PO4)8]∞ are arranged in a parallel mode, which greatly improves the polarizability of the phosphate. As a result, β-Li2RbBi(PO4)2 presents the largest SHG response, 5.2 × KH2PO4 (KDP), at 1064 nm incident radiation among all the Bi-based phosphates. Furthermore, first-principles calculations reveal that the synergistic effect of P–O and Bi–O groups contributes significantly to the optical properties of the title compound.
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CHEMISTRY-A EUROPEAN JOURNAL
·
2023
·Vol. 29
·期 59
·e202302132
·
IF 4.3
Jun-Tao Shi,Xian-Hui Chen,Yuan-Yuan Peng,Gui-Ping Wang,Guang-Yan Du,Quan Li
Graphical A novel mechanically bonded amphiphile (MBA)-bistable [2]rotaxane H2 was designed and prepared. In both the solution and aggregated states, H2 demonstrated pH-responsive emissions due to the presence of a distance-dependent photoinduced electron transfer (PET) process. Moreover, the morphology of H2 aggregates can be tuned from nanospheres to vesicles due to the pH-controlled shuttling motion-induced alternation of H2 amphiphilicity. Advanced Organic Chemical Materials Co-constructed Mechanically bonded amphiphiles (MBAs), also known as mechanically interlocked molecules (MIMs), have emerged as an important kind of functional building block for the construction of artificial molecular machines and soft materials. Herein, a novel MBA, i. e., bistable [2]rotaxane H2 was designed and synthesized. In the solution state, H2 demonstrated pH and metal ion-responsive emissions due to the presence of a distance-dependent photoinduced electron transfer (PET) process and the fluorescence resonance energy transfer (FRET) process, respectively. Importantly, the amphiphilic feature of H2 has endowed it with unique self-assembly capability, and nanospheres were obtained in a mixed H 2 O/CH 3 CN solvent. Moreover, the morphology of H2 aggregates can be tuned from nanospheres to vesicles due to the pH-controlled shuttling motion-induced alternation of H2 amphiphilicity. Interestingly, larger spheres with novel pearl-chain-like structures from H2 were observed after adding stoichiometric Zn 2+ . In particular, H2 shows pH-responsive emissions in its aggregation state, allowing the visualization of the shuttling movement by just naked eyes. It is assumed that the well-designed [2]rotaxane, and particularly the proposed concept of MBA shown here, will further enrich the families of MIMs, offering prospects for synthesizing more MIMs with novel assembly capabilities and bottom-up building dynamic smart materials with unprecedented functions.
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CELL
·
2022
·
IF 66.85
Liming Yan,Yucen Huang,Ji Ge,Zhenyu Liu,Pengchi Lu,Bo Huang,Shan Gao,Junbo Wang,Liping Tan,Sihan Ye,Fengxi Yu,Weiqi Lan,Shiya Xu,Feng Zhou,Lei Shi,Luke W. Guddat,Yan Gao,Zihe Rao,Zhiyong Lou
Summary Decoration of cap on viral RNA plays essential roles in SARS-CoV-2 proliferation. Here, we report a mechanism for SARS-CoV-2 RNA capping and document structural details at atomic resolution. The NiRAN domain in polymerase catalyzes the covalent link of RNA 5′ end to the first residue of nsp9 (termed as RNAylation), thus being an intermediate to form cap core (GpppA) with GTP catalyzed again by NiRAN. We also reveal that triphosphorylated nucleotide analog inhibitors can be bonded to nsp9 and fit into a previously unknown “Nuc-pocket” in NiRAN, thus inhibiting nsp9 RNAylation and formation of GpppA. S-loop (residues 50-KTN-52) in NiRAN presents a remarkable conformational shift observed in RTC bound with sofosbuvir monophosphate, reasoning an “induce-and-lock” mechanism to design inhibitors. These findings not only improve the understanding of SARS-CoV-2 RNA capping and the mode of action of NAIs but also provide a strategy to design antiviral drugs.
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ChemistrySelect
·
2022
·Vol. 7
·期 35
·e202202475
·
IF 2.307
Zhiqiang Zhang,Qingyan Chu,Yuan Sun,Hao Wang,Deming Lu,Yuying Liu,Haibin Xiao,Ping Wang,Hongyou Cui,Ming Wang
Graphical The hierarchical TS-1 with abundant mesopores was prepared by TPAOH/TIPA treatment, which was applied to the reaction of the ammoximation-oxidation of acetone to 2-nitropropane. Hierarchical TS-1 catalyst performed excellent reusability and catalytic activity with 97.5 % conversion and 96.8 % selectivity. The novel technology for clean and mild synthesis of nitroalkanes has been developed. Modified TS-1 catalysts for the acetone ammoximation-oxidation reaction were prepared by post-treating TS-1 with different concentrations of TPAOH/TIPA, and the effects of alkali treatment on the catalytic performance were systematically investigated. The techniques such as XRD, SEM, TEM and N 2 sorption were used to characterize the catalyst samples, which exhibited that the introduction of alkali increased the volume and quantity of mesopores, while there was no obvious influence on the framework structure and titanium content in the framework. And the improvement in catalyst performance after alkali treatment was attributed to the increase of mesopores and the decrease in diffusion limitation. In addition, three common ammoximation catalysts (Ti-MOR, Ti- MWW and TS-1) were systematically investigated in this reaction. Ti-MOR showed superiority in the ammoximation to oxime, while TS-1 displayed the best catalytic performance in the ammoximation oxidation of ketones to nitroalkanes. It was indicated that modified TS-1 could be a promising catalyst for the ammoximation-oxidation reaction of acetone.
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