mSystems
·
2020
·
IF 6.633
Yougui Li,Wenyi Xu,Fang Zhang,Shi Zhong,Yuqing Sun,Jinxi Huo,Jianxun Zhu,Chongming Wu
Hyperlipidemia is a worldwide epidemic with an obvious gender disparity in incidence. Modulations on gut microbiota by traditional Chinese medicines (TCM) are emerging as a potential rationale governing the profitable effects of drugs on hyperlipidemia. However, it is unclear how gut microbes regulate the progression of hyperlipidemia. Here, we found that mulberry leaf extract (MLE) and its active component 1-deoxynojirimycin (DNJ) diminished hyperglycemia and hypertriglyceridemia with similar efficacy in male and female mice but preferentially alleviated hypercholesterolemia in female mice. Further investigations showed that DNJ sex-specifically downregulated the expression of lipogenic genes, especially cholesterol-biosynthetic genes. Oral administration of DNJ imposed more profound modulation on gut microbiota in female mice than in male ones, as estimated by 16S rRNA metatranscriptomic analysis. DNJ markedly enriched Akkermansia and Clostridium group XIVa and promoted the production of indole-3-propionic acid (IPA) in a sexually dimorphic way. Importantly, IPA tightly associates with the antihyperlipidemic effect of DNJ and exhibited a potent lipid-lowering effect both in vitro and in vivo. Together, our results have established a regulatory mechanism by which DNJ sex-specifically improves hyperlipidemia, offering an in-depth theoretical basis for therapeutic exploitation of DNJ as a sex-specific intervention against hyperlipidemia.IMPORTANCE Hyperlipidemia has been intensively focused on by researchers around the world owing to its major contribution to cardiovascular diseases. Various evidence reveals that women are more susceptible than male counterparts to dyslipidemia, making sex-dependent therapeutic strategies and drugs urgently needed. In the present work, we demonstrate that DNJ, the main active component of mulberry leaves, exerts an obvious female-preferential antihyperlipidemic effect through specifically enriching Akkermansia and Clostridium XIVa and elevating an active microbial metabolite, indole-3-propionic acid (IPA), in female mice. Moreover, we have corroborated the potent lipid-lowering efficacy of IPA both in vitro and in vivo. These findings not only indicate a potential mechanism by which gut microbes and their metabolites confer the beneficial role of DNJ in ameliorating hyperlipidemia but also provide an in-depth theoretical basis for therapeutic exploitation of DNJ as a female-specific intervention against hyperlipidemia.
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DOI
PubMed
高影响因子
ORGANIC & BIOMOLECULAR CHEMISTRY
·
2020
·Vol. 18
·期 18
·3512-3521
·
IF 3.412
Shijie Wang,Dingyao Gao,Ke Li,Siqin Ye,Qingzhu Liu,Ying Peng,Gaochao Lv,Ling Qiu,Jianguo Lin
Early evaluation of the therapy efficiency can promote the development of anti-tumor drugs and optimization of the treatment method. Caspase-3 is a key biomarker for early apoptosis. Detection of caspase-3 activity is essential for quick assessment of the curative effect. We have reported a PET probe that could image drug-induced tumor apoptosis in vivo. However, high liver uptake limits its application. In order to optimize the pharmacokinetics of the previous probe, we introduced a hydrophilic peptide sequence to minimize liver uptake. The structure of the new probe was confirmed by mass spectrometry and nuclear magnetic resonance. This probe was able to cross the cell membrane freely and could be converted into a dimer through the condensation reaction of 2-cyano-6-aminobenzothiazole (CBT) and cysteine in response to intracellular activated caspase-3 and glutathione (GSH). The hydrophobic dimers further self-assembled into nanoparticles, which could enhance the probe aggregation in apoptotic tumor tissues. In vivo experiments showed that the tumor uptake of the new probe was higher than that of the previous probe, while the liver uptake of the new probe was significantly reduced. The new probe might be promising in imaging apoptotic tumors with suitable pharmacokinetics.
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高影响因子
ANALYTICAL CHEMISTRY
·
2014
·
IF 5.825
Rui Li,Kangbing Wu,Changxian Liu,Yin Huang,Yanying Wang,Huaifang Fang,Huijuan Zhang,Chunya Li
A novel ionic liquid, 4-amino-1-(3-mercapto-propyl)-pyridine hexafluorophosphate (AMPPH), was successfully synthesized and characterized. Subsequently, AMPPH was used as a functional monomer to fabricate AMPPH-modified gold nanoparticles (AMPPH–AuNPs) via a one-pot synthesis method. The as-prepared AMPPH–AuNPs were confirmed with transmission electron microscopy and X-ray photoelectron spectroscopy. AMPPH–AuNPs were used to construct a biocompatible interface to immobilize rabbit anti-human IgG (anti-HIgG) onto a glassy carbon electrode (GCE) surface, followed by a cross-linking step with glutaraldehyde to fabricate an anti-HIgG–AMPPH–AuNPs/GCE. The nonspecific binding sites were enclosed with bovine serum albumin (BSA) to develop an immunosensor for human IgG. Electrochemical impedance spectroscopy, cyclic voltammetry and differential pulse voltammetry were used to investigate the electrochemical properties of the developed immunosensor. The results indicate that AMPPH–AuNPs can improve the immunosensing performance. The current response of the immunosensor was found linearly related to human IgG concentration in the range of 0.1–5.0 ng mL–1 and 5.0–100.0 ng mL–1. The detection limit is estimated to be 0.08 ng mL–1 (S/N = 3). The obtained immunosensor was successfully applied to the analysis human IgG immunoglobulin in human serum, and the results were well consistent with ELISA method.
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PubMed
高影响因子
JOURNAL OF POWER SOURCES
·
2021
·
IF 9.127
Yingmin Jin,Xin Zong,Xuebai Zhang,Chaojun Liu,Dong Li,Zhenggang Jia,Gen Li,Xuanguang Zhou,Junhua Wei,Yueping Xiong
Composite solid electrolyte (CSE) consisting of Li + conductive fillers and polymer matrix has become attractive for high energy-density lithium batteries . Current researches focus on the “ceramic in polymer”-type CSE due to the severe agglomeration of ceramic particles. Consequently, the destroyed percolation network impedes the further improvement in ionic conductivity . In this work, we report a novel structural design of ceramic-enriched CSE consisting of three-dimensional (3D) interconnected Li 1.3 Al 0.3 Ti 1.7 (PO 4 ) 3 (LATP), polyvinylidene fluoride (PVDF) and LiN(SO 2 ) 2 (CF 3 ) 2 (LiTFSI). The 3D LATP network is constructed through the surface functionalization of LATP particles by polymethyl methacrylate (PMMA). Owing to the intermolecular interaction between PMMA and PVDF, the incorporation of PMMA-coated LATP (denoted as LATP@PMMA) enables the uniform distribution of ceramic phase and generates interconnected LATP network. Noticeably, such an electrolyte maximizes the utilization of inorganic fillers through the facile treatment of zero-dimensional materials, which is comparable to those 3D template-derived fillers. Furthermore, continuous Li + conduction pathways through LATP framework and LATP/PVDF interphase are guaranteed by the enhanced affinity of LATP towards PVDF matrix and the intrinsic Li + complexation capability of PMMA. Remarkable cycling performances of the LiNi 0.5 Co 0.2 Mn 0.3 O 2 /LATP@PMMA-PVDF/Li batteries confirm the feasibility of 3D ceramic-enriched composite electrolyte in the application of solid-state lithium batteries.
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高影响因子
CHEMICAL PHYSICS LETTERS
·
2020
·
IF 2.029
Lijun Yang,Dongxue Wu,Jun Tang,Yawei Hao,Jingchuan Wang,Xiaochong Zhao,Pan Yang
A simple and environment friendly method for the synthesization of CH 3 NH 3 BF 4 , which exhibits high purity and yield. With close ionic radius, BF 4 − was doped into CH 3 NH 3 PbBr 3 perovskite as substitution of Br − to improve the chemical stability of lead halide perovskite. The results show that the doped-BF 4 − can increase the charge carrier lifetime of CH 3 NH 3 PbBr x (BF 4 ) (3-x) perovskite thin films, meanwhile decrease the rate of recombination. This is mainly due to the larger ionic radius of BF 4 − , which brings about a lower degree X-ray diffraction angle shift, wider ultraviolet visible spectrophotometer absorbance band, red-shift photoluminescence emission peak, and longer fluorescence lifetime.
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DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Ziqi Li,Yuying Wang,Wengang Chen,Lili Zheng,Weimin Wan,Xi Wang
Targeted and stimuli-responsive drug delivery systems (DDS) hold significant potential for improving therapeutic efficacy and mitigating adverse effects in cancer treatment. Here, we developed a pH-responsive nanocarrier through a surface modification strategy, which enables active targeting of hepatocellular carcinoma (HCC) cells for the delivery of drug curcumin (Cur), a poorly water-soluble therapeutic agent. Briefly, a polydopamine (PDA) layer was first deposited onto the surface of cellulose nanocrystals (CNCs) via the self-polymerization of dopamine hydrochloride. Subsequently, 3-carboxyphenylboronic acid (CA) was grafted onto the PDA-coated surface to obtain CNCs@PDA-CA. After that, Cur was loaded onto CNCs@PDA-CA by forming a boronate ester linkage with the boronic acid groups of CA, resulting in the final nanocomposite CNCs@PDA-CA-Cur with a drug loading capacity of 10.58 ± 1.32 %. This nanocarrier not only exhibited active targeting ability toward HCC cells, but also achieved controlled Cur release in response to the acidic tumor microenvironment. Specifically, CNCs@PDA-CA-Cur showed high pH-responsive ability that released 3.18-folds of Cur in acidic environments (pH = 5.4) more than in neutral environments (pH = 7.2). Furthermore, the nanocarrier demonstrated efficient active targeting to HCC cells, leading to elevated intracellular Cur accumulation and enhanced cytotoxicity in both 2D and 3D HCC models in vitro. Collectively, these results highlight the potential of the targeted and pH-responsive nanocarrier to improve the therapeutic delivery of poorly soluble drugs for HCC treatment.
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF ORGANIC CHEMISTRY
·
2024
·
IF 3.3
Nan Wang,Yuanyuan Huang,You Zi,Mengke Wang,Weichun Huang
A practical and regioselective direct N-alkylation of 2-pyridones is enabled by use of α-keto esters in the P(NMe2)3-mediated deoxygenation process. The reaction proceeds under mild conditions to produce N-alkylated 2-pyridones with high selectivity and generality, and the protocol is shown to be applicable for the scale-up synthesis, which makes it promising for practical applications.
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PubMed
高影响因子
SYNTHESIS-STUTTGART
·
2019
·Vol. 51
·期 21
·4048-4065
·
IF 2.867
Rui-Xiang Wang,Zhuang Fang,Guanyinsheng Qiu,Wenlin Xie,Jin-Biao Liu
The copper-catalyzed synthesis of 3-vinylisocoumarin-1-imine is reported. The transformation proceeds smoothly with good yields in THF and the regioselectivity was determined by a O-nucleophilic 6-endo cyclization. Studies on the mechanism indicate that copper trifluoroacetate serves as a Lewis acid, and the use of vinyl-connected 2-alkynylbenzamide is important for this O-nucleophilic 6-endo cyclization.
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高影响因子
Evidence-based Complementary and Alternative Medicine
·
2021
·
IF 2.63
Yuling Tong,Manyi Jing,Xu Zhao,Honghong Liu,Shizhang Wei,Ruisheng Li,Xia Liu,Min Wang,Hongtao Song,Yanling Zhao
The classic prescription Zuojin Pill (ZJP) shows a good therapeutic effect on chronic atrophic gastritis (CAG); it is of great significance to clarify its specific mechanism. Therefore, we explore the mechanism of ZJP on MNNG-induced CAG by integrating approaches. First of all, through the pathological changes of gastric tissue and the expression level of PGI and PGI/II in serum, the expression of inflammation-related factors was determined by RT-PCR to determine the efficacy. Then, UPLC-Q-TOF/MS was used for plasma and urine metabolomic analysis to screen the specific potential biomarkers and metabolic pathway of ZJP in ameliorating CAG and to explore its possible mechanism. ZJP significantly ameliorate the pathological injury of gastric tissue, increase levels of PGI and PGI/II, and reduce the expression level of proinflammatory factors. Through metabolomic analysis, 9 potential metabolic differences were identified and 6 related metabolic pathways were enriched. These findings indicate for the first time the potential mechanism of ZJP in improving CAG induced by MNNG and are of great significance to the clinical development and application of ZJP-related drugs.
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高影响因子
Frontiers in Chemistry
·
2022
·
IF 5.545
Yin Zhen-Hua,Liu Xiao-Peng,Wang Jin-Mei,Xi Xue-Feng,Zhang Yan,Zhao Rui-Lin,Kang Wen-Yi
Pleurotus placentodes, a fungus, belongs to the Pleurotaceae family. The aim of the present study was to characterize the structure of a novel polysaccharide from fruiting bodies of P. placentodes (PPp-W) and evaluate its anticoagulant activity in vitro. The high-performance liquid chromatography and GC–MS analysis indicated that PPp-W with a molecular weight of 27.4 kDa was mainly composed of mannose (17.56%), glucose (6.37%), galactose (44.89%), and fucose (1.22%) with a certain amount of 3-O-methyled galactose. SEM, XRD, and AFM combined with Congo red test revealed that PPp-W was an irregular curly sheet with triple-helix conformation. The FT-IR, methylation, and nuclear magnetic resonance analysis indicated that PPp-W contained→6)-α-D-Galp-(1→, →6)-3-O-Me-α-D-Galp-(1→and →2, 6)-α-D-Galp-(1→ as main chain, partially substituted at O-2 and O-6 by non-reducing ends of β-D-Manp-(1→ and β-L-Fucp-(1→ with a small amount of α-1,3-linked-Glcp in backbone. PPp-W could significantly prolong APTT (12.9 ± 0.42 s, p < 0.001) and thrombin time (39.9 ± 0.28 s, p < 0.01) compared with the control group (11.45 ± 0.071 s and 38.05 ± 0.21 s), which showed that PPp-W had anticoagulant activity. These studies suggested that PPp-W was a 3-O-methylated heteroglycan and might be suitable for functional foods and natural drugs as an anticoagulant ingredient, which provided a basis for the application of polysaccharides from P. placentodes.
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PubMed
高影响因子
CRYSTENGCOMM
·
2023
·Vol. 25
·期 36
·5085-5091
·
IF 3.1
Zhi-Yuan Yue,Rong-Meng Liao,Wang Luo,Na Wang,Le-Ping Miao,Heng-Yun Ye,Chao Shi
Organic–inorganic hybrid molecular ferroelectrics have attracted broad attention in the field of research of ferroelectric materials. Following the surge of stable candidates, hybrid bimetal molecular-based materials have been recently explored to achieve excellent ferroelectricity. However, rare hybrid bimetal molecular ferroelectrics were reported. Inspired by “ferroelectrochemistry”, a chiral nitrite-based hybrid bimetal molecular ferroelectric material, [(R)-3-quinuclidinol]2[LiCo(NO2)6] (1), was reported. The ferroelectric-paraelectric phase transition (C2 ↔ P321) behaviour of 1; was found at 343 K (Curie temperature, Tc) with a spontaneous electronic polarization (Ps) of 0.52 μC cm−2 and a coercive field (Ec) of 48 kV cm−1 at 298 K. Various-temperature single crystal structural analysis and dielectric constant measurements reveal that the rapid rotation of the organic cation and slight order–disorder transition of the NO2− anion are the origin of the ferroelectricity. To our knowledge, 1 is the first example of a nitrite-based hybrid bimetal molecular ferroelectric material that is triggered by an external electric field. This finding establishes a clear stimulus–structure–response relationship in advanced hybrid ferroelectric materials.
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高影响因子
APPLIED CATALYSIS B-ENVIRONMENTAL
·
2019
·
IF 14.229
Bingbing Hu,Maocong Hu,Qiang Guo,Kang Wang,Xitao Wang
In this work, a series of In(OH) 3 with abundant indium vacancies were synthesized by hydrothermal method using mixed solvent of ethylenediamine(En) and water for photocatalytic reduction of CO 2 with H 2 O vapor. The morphology, particle size, crystallinity, pore structure, and vacancy concentration of photocatalysts were successfully regulated by adjusting the ratio of En and water in mixed solvent, which were characterized by XRD, FT-IR, TG-DTA, SEM, HRTEM, UV–vis DRS, PL spectra, XPS, and EPR. Moreover, the effect of En/water ratio on the adsorption capacity to CO 2 and photo-electrical properties of In(OH) 3 were further investigated by in-situ FTIR, PL spectra, UV–vis DRS, CV, and EIS. In(OH) 3 prepared in mixed solution exhibited much higher photocatalytic activity for CO 2 reduction with H 2 O vapor compared to that prepared with water only solvent. The enhanced photocatalytic performance can be attributed to the special morphology, smaller particle size, larger BET surface area, and especially generated indium vacancies creating defect energy levels in the bandgap, which improved visible light absorption, carrier separation, and CO 2 chemisorption. This work paves the way to enable a non-visible-light response material with high visible-light photocatalytic activity by regulating vacancy concentration via a facile method.
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高影响因子
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