ANALYTICAL BIOCHEMISTRY
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2014
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IF 2.305
Hui Liu,Yong-Sheng Wang,Jia-Cheng Wang,Jin-Hua Xue,Bin Zhou,Hui Zhao,Shan-Du Liu,Xian Tang,Si-Han Chen,Ming-Hui Li,Jin-Xiu Cao
A highly sensitive, low-cost colorimetric aptasensor was developed for the determination of 8-hydroxy-2′-deoxyguanosine (8-OHdG) in human urine. The method is based on a conformational switching of the 8-OHdG aptamer to form a G-quadruplex structure in the presence of 8-OHdG. The resulting G-quadruplex assembles into a peroxidase-like DNAzyme with hemin , which effectively catalyzes the oxidation of 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS 2− ) by H 2 O 2 to ABTS + , resulting in an increase in the absorption signal at 416 nm along with a color change of the solution. The response signals linearly correlated with the concentration of 8-OHdG, ranging from 466 pM to 247 nM with a detection limit of 141 pM. The relative standard deviation and the recovery were 1.97–3.47% ( n = 11) and 98.8–100.2%, respectively. The proposed method avoids the label and derivatization steps in common methods and allows direct analysis of the samples by the naked eye without costly instruments, which is reliable, inexpensive, and sensitive.
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高影响因子
INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
·
2014
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IF 2.235
Lu Lin,Tong Zhang,Xiongfu Zhang,Haiou Liu,King Lun Yeung,Jieshan Qiu
In this work, we fabricated a new type of Pd/SiO2@ZIF-8 core–shell catalyst composed of a Pd/SiO2 microsphere as the core and a ZIF-8 membrane as the shell. The preparation procedure involves first loading Pd nanoparticles onto nonporous SiO2 microspheres to obtain the Pd/SiO2 core and then coating a perfect layer of ZIF-8 shell on the core by electrostatic induction to form a Pd/SiO2@ZIF-8 core–shell structure. It is crucial that the modification of the Pd/SiO2 core with polyelectrolyte was employed to improve the charge distribution of the core surface and preferably adsorb Zn2+ cations in the synthesis solution so as to assist the formation of a continuous ZIF-8 shell. The compactness and thickness of the shell were optimized by increasing the number of cycles of ZIF-8 growth. The hydrogenations of alkenes with different molecular sizes showed that the Pd/SiO2@ZIF-8 catalysts can provide excellent product selectivity and antipoisoning and antileaching properties for the hydrogenations of alkenes.
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JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
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2015
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IF 2.979
Hui Liu,Yong-Sheng Wang,Xian Tang,Hui-Xian Yang,Si-Han Chen,Hui Zhao,Shan-Du Liu,Yu-Feng Zhu,Xiao-Feng Wang,Yan-Qin Huang
A sensitive, lable-free and low cost fluorescence aptasensor was developed for detecting 8-hydroxy-2′-deoxyguanosine (8-OHdG) by using 8-OHdG aptamer (Apt) as a recognition probe and N-methyl mesoporphyrin IX (NMM) as a reporter. The method is based on the conformational switching of a K + -stabilized G-quadruplex to a 8-OHdG-stabilized one. NMM can selectively bind to K + -stabilized G-quadruplex instead of 8-OHdG-stabilized one. The addition of 8-OHdG in the solution of Apt - K + ions leads to a sharp change in fluorescence intensity, which showed a good linear response toward 8-OHdG concentration ranging from 3.96 nM to 211 nM with a detection limit of 1.19 nM. The relative standard deviation and the recovery were 1.23–3.26% ( n = 11) and 94.8–106.7%, respectively. The proposed aptasensor consists of only an aptamer probe and a specific dye NMM, avoiding the complex and expensive labeling procedure. Thus it is much cheaper and more applicable.
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PLoS One
·
2015
·Vol. 10
·期 9
·e0137066
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IF 3.234
Yan Zhang,Junling Shi,Laping Liu,Zhenhong Gao,Jinxin Che,Dongyan Shao,Yanlin Liu
Pinoresinol diglucoside (PDG) and pinoresinol (Pin) are normally produced by plant cells via the phenylpropanoid pathway. This study reveals the existence of a related pathway in Phomopsis sp. XP-8, a PDG-producing fungal strain isolated from the bark of the Tu-chung tree ( Eucommiaulmoides Oliv.). After addition of 0.15 g/L glucose to Phomopsis sp. XP-8, PDG and Pin formed when phenylalanine, tyrosine, leucine, cinnamic acid, and p -coumaric acid were used as the substrates respectively. No PDG formed in the absence of glucose, but Pin was detected after addition of all these substrates except leucine. In all systems in the presence of glucose, production of PDG and/or Pin and the accumulation of phenylalanine, cinnamic acid, or p -coumaric acid correlated directly with added substrate in a time- and substrate concentration- dependent manner. After analysis of products produced after addition of each substrate, the mass flow sequence for PDG and Pin biosynthesis was defined as: glucose to phenylalanine, phenylalanine to cinnamic acid, then to p -coumaric acid, and finally to Pin or PDG. During the bioconversion, the activities of four key enzymes in the phenylpropanoid pathway were also determined and correlated with accumulation of their corresponding products. PDG production by Phomopsis sp. exhibits greater efficiency and cost effectiveness than the currently-used plant-based system and will pave the way for large scale production of PDG and/or Pin for medical applications.
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TALANTA
·
2024
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IF 5.6
Jiayue Sun,Lei Ji,Yan Li,Xiyue Cao,Xiuling Shao,Jianfei Xia,Zonghua Wang
In this research, self-screening aptamer and MOFs-derived nanomaterial have been combined to construct electrochemical aptasensor for environmental detection. By utilizing the large specific surface area of reduced graphene oxide (rGO), ZIF-8 was grown in situ on surface of rGO, and the composites was pyrolyzed to obtain MOFs-derived porous carbon materials (rGO-NCZIF). Thanks to the synergistic effect between rGO and NCZIF, the complex exhibits remarkable characteristics, including a high electron transfer rate and electrocatalytic activity . In addition, the orderly arrangement of imidazole ligands within ZIF-8 facilitated the uniform doping of nitrogen elements into the porous carbon, thereby significantly enhancing its electrochemical performance . After carboxylation , rGO-NCZIF was functionalized with self-screening aptamer for fabricating electrochemical aptasensor, which can be used to detect Erwinia cypripedii , a kind of quarantine plant bacteria, with detection limit of 4.92 × 10 3 cfu/mL. Due to the simplicity and speed, the aptasensor is suitable for rapid customs inspection and quarantine. Additionally, the universality of this sensing strategy was verified through exosomes detection by changing the aptamer. The results indicated that the rGO-NCZIF-based electrochemical aptasensor had practical value in the environmental and medical fields.
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Journal of Environmental Chemical Engineering
·
2024
·
IF 7.4
Shuqin Zhang,Jieni Wang,Chenlin Wei,Peiliang Liu,Shumeng Qin,Leichang Cao,Yanmei Zhou,Jinglai Zhang,Shicheng Zhang
Global warming and the increasing frequency of extreme weather caused by the gradual increase in CO 2 concentration in the atmosphere are urgent issues that must be addressed. In this study, ZIF-8-derived in-situ nitrogen-doped porous carbon materials were prepared through one-step pyrolysis of ZIF-8. The synthesis was gradually optimized by exploring different pyrolysis temperatures to determine the optimal ZIF-8-derived porous carbon for CO 2 adsorption. The CO 2 -adsorption capacity of ZIF-8–800 reached 5.22 and 6.19 mmol g −1 at 25 ℃ and 0 ℃ (1 bar), respectively. After five cycles, its CO 2 -adsorption capacity decreased by only about 1.7 %. Moreover, the CO 2 /N 2 selectivity at 1 bar was as high as 21.2 (48.9 at 0.05 bar), and the isothermal adsorption heat was also within a moderate range (31 kJ mol −1 ). The superior CO 2 adsorption performance was found to originate from the comprehensive effect of the three-dimensional pore structure that combined reasonably coordinated micropores, mesopores, and macropores, as well as some specific favorable N/O groups. The results indicate the broad application prospects of ZIF-8-Ts in CO 2 capture and separation and provide new ideas for the sustainable application of novel MOF-derived porous carbon materials.
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SEPARATION AND PURIFICATION TECHNOLOGY
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2024
·
IF 8.1
Jiayan Zhu,Lin Zhang,Zongjian Liu,Wenxia Zhai,Zhengfei Ma,Qun Cui,Haiyan Wang
The separation of mono- and di-branched C6 alkane isomers is one of the most challenging industrial processes for the production of high-octane gasoline components. In this work, ZIF-8 with dual ligands of imidazole (IM) and 2-methylimidazole (2-MIM), named as DLA-ZIF-8-IM, was synthesized by the method of delayed linker addition, and the microstructure of DLA-ZIF-8-IM was analyzed. Adsorption thermodynamic, kinetic and separation properties of C6 alkane isomers were investigated. The results show that compared with SALE-ZIF-8-IM prepared by the solvent-assisted ligand exchange method, the synthesis method of DLA-ZIF-8-IM is simpler and the BET specific surface area and pore volume (2136 m 2 ·g −1 and 0.72 cm 3 ·g −1 ) of DLA-ZIF-8-IM are increased by 21 % and 14 %, which is more conducive to adsorption and diffusion of 3-methylpentane (3MP) on DLA-ZIF-8-IM. The equilibrium adsorption capacity of 3MP on DLA-ZIF-8-IM reaches up to 2.89 mmol·g −1 , which is 1.1 times than that on SALE-ZIF-8-IM, and the ratio of diffusion time constants between 3MP and 2,3-dimethylbutane (23DMB) on DLA-ZIF-8-IM (5.13) is 2.2 times than that on SALE-ZIF-8-IM. Moreover, at 30℃, the breakthrough adsorption capacity of 3MP on DLA-ZIF-8-IM is 1.46 mmol·g −1 , which is 1.6 times more than that of SALE-ZIF-8-IM (0.90 mmol·g −1 ), while the breakthrough adsorption capacity of 23DMB is basically the same. The separation factor between 3MP and 23DMB on DLA-ZIF-8-IM is 2.96 via the synergy of thermodynamic and kinetic mechanism and the dynamic adsorption capacity of 3MP reaches 1.46 mmol·g −1 . DLA-ZIF-8-IM achieves complete separation of C6 alkane mixtures for obtaining single high-purity product. This work provides a novel synthesis strategy for developing C6 alkane (clear) adsorption separation materials by modulating microporous structures of dual-ligand MOFs.
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COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
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2025
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IF 5.4
Yashi Wang,Zhaoguo Wu,Junwei Yang,Aiwu Zhao,Jin Wang
In order to efficiently keep activities of enzymes and enhance stabilities of enzymes, intercalation of enzymes into core of Au nanorod and MOFs shell has been designed. Vice versa, enzymes immobilized on the surface of Au nanorod can guide MOFs shell growth via biomineralization interplay accompany with surfactant directed growth of MOFs on an anisotropic plasmonic template, leading to formation of a porous layer-by-layer nanostructure. Due to incorporation of stimuli-responsive enzyme and MOFs skeleton, loaded anticancer drug can be efficiently released under endogenous stimuli. In-situ SERS and UV-Vis analysis indicate that porous Au nanorod@enzymes@ZIF-8 nanocomposites can perform superior drug releasing behavior compared to Au nanorod@ZIF-8. Hence, the porous Au nanorod@enzymes@ZIF-8 nanocomposites can give more efficient enzymatic biocascade reaction in the nanoreactor in contrast with Au nanorod@ZIF-8@enzymes. Based on porous characteristics of ZIF-8 shell in the Au nanorod@enzymes@ZIF-8 nanocomposites, the nanocomposites can cause complete apoptosis of HeLa cell via enzymatic biocascade reaction and loaded anticancer drug. It can be expected that the unique biological-inorganic-organic hybridized nanosystem would be extensively applied in quantitative evaluation of multi-modal tumor therapy.
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Materials Today Chemistry
·
2025
·
IF 6.7
Huaiyin Chen,Zhonghe Wang,Jun Li,Xiaolin Liu,Congxiang Li,Yongzhao Li,Weihe Yu,Longmin Liu,Yi Zhang,Yuekun Lai,Weilong Cai
ZIF-8 is considered as a promising functional material in anti-corrosion coating applications. However, ZIF-8 hydrolyzes easily, especially in acidic and salty media, due to its unstable Zn–N coordination bonding and hydrophilic crystal surface, which reduces anti-corrosion durability of the coatings. In this study, highly hydrophobic modification of ZIF-8 particles was performed through simple surface ligand exchange with 5,6-dimethylbenzimidazole (DMBIM). The as-prepared ZIF-8/DMBIM particles showed high water-repellency and enhanced chemical stability in acidic, neutral, and alkaline media, and excellent compatibility and barrier function in epoxy coatings. As a consequence, ZIF-8/DMBIM modified epoxy coatings showed significantly enhanced corrosion resistances in acid, alkali and salt aqueous solutions with pH 3.0–11.0. Electrochemical impedance spectroscopy tests demonstrated that, after 20 days of immersion in 3.5 wt% NaCl solution at all the studied pH, the |Z| 0.01 Hz values of ZIF-8/DMBIM modified epoxy coatings were approximately two orders of magnitude higher than of the original epoxy coatings. This work provides an idea for the development and application of ZIF materials in more fields, and innovative anti-corrosion coatings based on functional modification of materials.
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MOLECULES
·
2024
·Vol. 29
·期 24
·6011
·
IF 4.2
Ting Zhou,Luyao Zang,Xia Zhang,Xia Liu,Zijie Qu,Guodong Zhang,Xiufeng Wang,Fang Wang,Zhiqing Zhang
Gold nanoclusters (AuNCs) have been widely investigated because of their unique photoluminescence properties. However, the applications of AuNCs are limited by their poor stability and relatively low fluorescence. In the present work, we developed nanocomposites (L-Cys-AuNCs@ZIF-8) with high fluorescence and stability, which were constructed by encapsulating the water-dispersible L-Cys-AuNCs into a ZIF-8 via Zn2+-triggered growth strategy without high temperature and pressure. The maximum emission wavelength of the L-Cys-AuNCs@ZIF-8 composite was at 868 nm, and the fluorescence intensity of L-Cys-AuNCs@ZIF-8 was nearly nine-fold compared with L-Cys-AuNCs without the ZIF-8 package. The mechanism investigation by fluorescence spectroscopy and X-ray photoelectron spectroscopy showed that L-Cys-AuNCs@ZIF-8 impeded ligand rotation, induced energy dissipation, and diminished the self-quenching effect, attributing to the spatial distribution of L-Cys-AuNCs. Based on the high fluorescence efficiency of L-Cys-AuNCs@ZIF-8, a “signal off” detective platform was proposed with copper ions as a model analyte, achieving a sensitive detection limit of Cu2+at 16.7 nM. The quenching mechanism was confirmed, showing that the structure of the L-Cys-AuNCs@ZIF-8 nanocomposites was collapsed by the addition of Cu2+. Attributing to the strong adsorption ability between copper ions and pyridyl nitrogen, the as-prepared L-Cys-AuNCs@ZIF-8 was shown to accumulate Cu2+, and the Zn2+in ZIF-8 was replaced by Cu2+.
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COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2025
·
IF 5.4
Jufen Yan,Lu Dai,Pei Wang,Yongsheng He,Qi Wang,Yiliang Chen,Haowei Zhang,Abdallah S. Ali,Rasha H. Ahmed,Ivana Radojcic Redovnikovic,Kui Zhang,Hongliang Wang,Xinya Han
The development of multifunctional materials capable of serving both as high-efficiency adsorbents and high-performance supercapacitors is a critical challenge for environmental and energy applications. In this study, a novel zeolitic imidazole framework-8/mycelial pellet (ZIF-8/MP) composite was successfully synthesized using a simple in-situ growth and staged adsorption process. The MP with its three-dimensional porous structure, served as a scaffold, facilitating the uniform distribution of ZIF-8 across the porous carbon matrix. The resulting ZIF-8 in the ZIF-8/MP composite demonstrated a remarkable adsorption capacity for Congo red (CR), with a maximum capacity of 1428.57 mg/g. The adsorption mechanism was attributed to electrostatic interactions, zinc coordination, hydrogen bonding, and π–π conjugation, as confirmed by FTIR, XPS, and DFT analyses. After adsorption, the carbonized ZIF-8/MP composite demonstrated outstanding supercapacitor performance, achieving a specific capacitance of 113.4 F/g at 1 A/g, and maintaining 93 % capacitance retention after 5000 charge/discharge cycles. Additionally, the ZIF-8/MP composite effectively reduced the toxicity of CR, as demonstrated by phytotoxicity assays, underscoring its potential for environmental remediation. This innovative approach provides an eco-friendly, easily separable, efficient, and practical multifunctional material for transforming hazardous waste into valuable resources for environmental detoxification and energy storage applications.
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INDUSTRIAL CROPS AND PRODUCTS
·
2024
·
IF 5.6
Jiangtao Dong,Hexin Li,Guoquan Wang,Haoming Li,Yanli Zhao,Xinan Li,Li Xu,Feng Zhou,Runqiang Liu
Pesticides play an important role in controlling plant diseases and insect pests to increase food production. Pyrethrin (Py), as a plant-derived insecticide, can effectively control wheat aphids owing to its fast knockdown, low concentration, and broad-spectrum insecticidal activity. However, Py is sensitive to UV irradiation and decomposes easily under sunlight, which leads to the loss of insecticidal activity. In this paper, we prepared one kind of metal organic framework nanopesticides based on zeolite imidazole-8 framework (ZIF-8) loaded with Py (Py@ZIF-8) via one-pot method for wheat aphid control. The prepared Py@ZIF-8 nanopesticides appeared acidic/basic pH and PO 4 3- stimuli-responsive properties for the controlled release of packaged Py by the disassembly of MOF skeleton and enhanced leaf wettability. The control efficacies of Py@ZIF-8 nanopesticides against wheat aphids reached ca. 86.67 ± 4.56 % under normal conditions, ca. 82.56 ± 3.88 % after water washing, and ca. 78.92 ± 4.34 % under exposure to UV irradiation, which appeared remarkably improvement compared with technical Py under UV irradiation and rain erosion condition, respectively. Even through the nanopesticides inevitably enter into soil or water, they appear good biosafety on wheat and cucumber seedlings by seed dressing, as well as zebrafish ( D. rerio ). Importantly, the eco-friendly nanocarriers will be ultimately degraded against bioaccumulation in crops based on the stimuli-responsive mechanisms. These results indicated that the prepared nanopesticides have an efficient wheat aphid control owing to the intelligent control release performance, as well as good UV and washing resistance, and good biosafety on non-target species, which have potential applications in agriculture against wheat aphids.
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