JOURNAL OF COLLOID AND INTERFACE SCIENCE
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2025
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IF 9.7
Xinzhi Wang,Jing Jiang,Jiayi Zhang,Qiyao Zeng,Aike Liu,Lunhong Ai
Defect engineering is considered one of the most powerful strategies for regulating the catalytic activity of electrocatalysts. A deep understanding of the defect-involved mechanism in electrocatalytic process is of great importance but remains a challenging task. In this study, an anionic Se-vacancy (V Se ) was introduced into iron diselenide (FeSe 2 ) nanoarrays, enabling the catalyst to exhibit improved electrocatalytic performance for sulfion oxidation reaction (SOR). The Se vacancy incorporated can modulate surface electronic structure and optimize the proximity of the d-p band center, strengthening the interaction between FeSe 2 -V Se and S atom. This endows the FeSe 2 /IF-V Se catalyst with the excellent electrocatalytic activity for SOR. As an alternative reaction to oxygen evolution reaction (OER), the low-potential SOR enables an ultralow electricity consumption in SOR-hydrid seawater electrolysis, avoiding detrimental chlorine electrochemistry. A proof-of-concept self-powered system based on FeSe 2 -V Se electrodes was constructed by integrating Zn-nitrite battery and SOR-hybrid seawater electrolyzer to produce high-value hydrogen, sulfur and ammonia. This work offers a fascinating strategy for the construction of an advanced energy system to produce hydrogen while utilizing seawater and wastewater.
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APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
·
2021
·Vol. 193
·期 7
·2029-2042
·
IF 2.926
Xu Jian-Miao,Cao Hui-Ting,Wang Ming,Ma Bao-Jian,Wang Liu-Yu,Zhang Kai,Cheng Feng,Xue Ya-Ping,Zheng Yu-Guo
d -amino acid oxidase (DAAO) is widely used in the industrial preparation of l -amino acids, and cultivating Escherichia coli ( E. coli ) expressing DAAO for the biosynthesis of l -phosphinothricin ( l -PPT) is very attractive. At present, the biomass production of DAAO by fermentation is still limited in large-scale industrial applications because the expression of DAAO during the fermentation process inhibits the growth of host cells, which limits higher cell density. In this study, the factors that inhibit the growth of bacterial cells during a 5-L fed-batch fermentation process were explored, and the fermentation process was optimized by co-expressing catalase (CAT), by balancing the biomass and the enzyme activity, and by adding exogenous d -alanine ( d -Ala) to relieve the limitation of DAAO on the cells and optimize fermentation. Under optimal conditions, the DO-STAT feeding mode with DO controlled at 30% ± 5% and the addition of 27.5 g/L lactose mixed with 2 g/L d -Ala during induction at 28 °C resulted in the production of 26.03 g dry cell weight (DCW)/L biomass and 390.0 U/g DCW specific activity of DAAO; an increase of 78% and 84%, respectively, compared with the initial fermentation conditions. The fermentation strategy was successfully scale-up to a 5000-L fermenter.
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BIOCHEMICAL ENGINEERING JOURNAL
·
2024
·
IF 3.7
Yun Liu,Ao Huang,Xiaowei Wen
Phospholipase D (PLD) is essential for the bioconversion of phosphatidylcholine (PC) to phosphatidylserine (PS), a process valuable in functional food and medicine. This study explores the stability and catalytic properties of PLD immobilized on chitosan-encapsulated magnetic nanoparticles (CMNPs), utilizing oxidized dextran (DX) and glutaraldehyde (Glu) as cross-linkers. The cross-linker concentration and immobilization time were optimized to assess their effects on PLD catalytic performance. PLD immobilized on CMNPs with DX (DX-CMNPs-PLD) exhibited optimal activity at pH 8.0 and 30 °C, retaining over 40 % activity after 14 cycles, while Glu-cross-linked PLD (Glu-CMNPs-PLD) retained approximately 65 %. DX-CMNPs-PLD demonstrated superior pH, temperature, and operational stability compared to free PLD. Additionally, the immobilized PLD was characterized using transmission electron microscopy, X-ray diffraction, and Fourier-transform infrared spectroscopy. Kinetics parameters (V max and K m ) of the immobilized PLD were also studied with free PLD serving as a control. Conformational analyses indicated a significant change in PLD's secondary structure, particularly in β-sheet content, which likely contributed to the enhanced stability and activity. These findings suggest a promising approach for PLD immobilization on CMNPs, with notable implications for biotechnological applications.
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INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
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2025
·
IF 8.3
Yixin Sun,Jiawen Liu,Xinyang Xu,Zhonghua Li
In the strategies of modifying g-C 3 N 4 by changing its electron transport structure, it has been demonstrated that constructing donor-acceptor (D-A) or D-π-A g–C 3 N 4 –based conjugated copolymers can obviously accelerate the separation of charge carriers. Inspired by their advantages, a novel g–C 3 N 4 –based D-A 1 -π-A 2 type polymer was designed and synthesized via the thermal copolymerization of urea and PA-PM (the product of the amidation reaction between PA and PM). As a result, the prepared CN-PA-PM photocatalysts with D-A 1 -π-A 2 structure not only improve visible-light response, but also promote the separation of charge carriers, thereby achieving superior photocatalytic hydrogen evolution activity. The optimized CN-PA-PM-13 polymer has the best hydrogen evolution efficiency of 17.79 mmol g −1 h −1 under visible light (λ > 420 nm), which is 33.7 times that of pure g-C 3 N 4 . This work presents a new design concept for g–C 3 N 4 –based polymer photocatalysts with high optical absorption capability and effective carrier separation ability.
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Frontiers in Microbiology
·
2024
·
IF 4
Yuhan Zheng,Feiyang Sun,Siyi Liu,Gang Wang,Huan Chen,Yongxin Guo,Xiufeng Wang,Maia Lia Escobar Bonora,Sitong Zhang,Yanli Li,Guang Chen
Background: The production of D-lactic acid (D-LA) from non-detoxified corn stover hydrolysate is hindered by substrate-mediated inhibition and low cell utilization times. In this study, we developed a novel temperature-sensitive hydrogel, F127-IEA, for efficient D-LA production using a cell-recycle batch fermentation process.Results: F127-IEA exhibited a porous structure with an average pore size of approximately 1 μm, facilitating the formation of stable Lactobacillus bulgaricus clusters within the gel matrix. It also maintains excellent mechanical properties. It also maintains excellent mechanical properties. F127-IEA immobilized Lactobacillus bulgaricus T15 (F127-IEA-T15) can be used in cell-recycle fermentation for over 150 days from glucose and 50 days from corn stover hydrolysate, achieving high production rates of D-LA from glucose (2.71 ± 0.85 g/L h) and corn stover hydrolysate (1.29 ± 0.39 g/L h). F127-IEA-T15 enhanced D-LA production by adsorbing and blocking toxic substances present in corn stover hydrolysate that are detrimental to cellular activity.Conclusions: The newly developed hydrogels in this study provide a robust platform for large-scale extraction of D-LA from non-detoxified corn stover.
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FOOD CHEMISTRY
·
2025
·
IF 9.8
Han Zhang,Heping Cui,Xue Xia,Shahzad Hussain,Khizar Hayat,Xiaoming Zhang,Chi-Tang Ho
Exogenous xylose exhibited a significant temperature-dependent regulatory effect on the furans and pyrazines formation during the degradation of dual-glycated Amadori rearrangement product of lysine (Lys), N α ,N ε -di(1-deoxy- d -xylulos-1-yl)lysine ( N α ,N ε -di-Xul-Lys ARP). At 100 °C, xylose primarily enhanced the dehydration pathway of deoxypentosone. This significantly increased furans yield, while delaying lysine release by interacting with mono-glycated Lys-ARPs, thereby inhibiting the early formation of pyrazines. 120 °C accelerated the dehydration and retro-aldolization of deoxypentosone, markedly boosting furans formation and further promoting the substantial production of α -dicarbonyl compound with extra-added xylose. Additionally, xylose buffered the rapid pH decline, enhancing the α -aminoketones electrophilicity and facilitating pyrazines formation in N α ,N ε -di-Xul-Lys ARP. At 140 °C, xylose hindered furans formation but notably increased methylpyrazine levels during 30–60 min. Furthermore, increasing the xylose concentration to 60 mmol/L at 120 °C markedly enhanced glyoxal and methylglyoxal production, yielding the highest pyrazine concentration of 98.04 μg/L within 60 min.
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JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2026
·
IF 9.6
Shixuan Yu,Shiying Fan,Shaomin Liu,Xinyong Li
The direct photocatalytic oxidation of inert methane (CH 4 ) to high-value-added oxygenates remains a significant challenge. This study precisely controls generated reactive oxygen species (ROS) types by modulating interfacial electronic interactions in Zn-Pt δ− -PS photocatalysts, promoting CH 4 conversion into formaldehyde (HCHO). Under ambient conditions, a HCHO production rate reached 3308 μmol·g −1 with a high selectivity of 77%. In situ spectroscopy and theoretical studies demonstrated that the elevated Pt d-band center in Pt-*OOH (* represents the adsorption state) lowered the occupancy of antibonding orbitals, thereby enhancing the interaction between Pt and *OOH, markedly polarizing the O O bond, facilitating its cleavage, and enabling the preferential in situ generation of •OH rather than •OOH. Furthermore, adequate •OH radical accelerated activation of the C H bond in CH 4 and induced the cleavage of its O H bond in methanol (CH 3 OH), promoting HCHO formation. This strategy may guide the rational design of the catalyst's electronic structure to enhance CH 4 photocatalytic oxidation. Download: Download high-res image (175KB) Download: Download full-size image
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Food Bioscience
·
2026
·
IF 6.2
Yucheng Li,Bo Fan,Yu-Cai He
E. coli P8 expressing a glycosyltransferase from Panax ginseng was constructed. • Coupling glycosyltransferase and sucrose synthase (SS) was adopted to yield Reb D. • Regeneration of UDPG in situ from UDP and sucrose was used to convert Reb A to Reb D. • DES (ChCl:glycerol=1:2) –H 2 O (4:6, v/v) enhances the conversion of Reb A to Reb D. • P8 cell and SS could convert 19.3 g/L Reb A to Reb D (21.0 g/L) in 8 h. Rebaudioside A (Reb A) can be converted into Rebaudioside D (Reb D) through a glycosylation reaction. Reb D is a higher-value sweetener with a very low caloric value, a glycemic index of zero, and greater sweetness than Reb A. However, its naturally low abundance limits large-scale production. Accordingly, this study developed an efficient bioconversion system for Reb D production. Two recombinant Escherichia coli strains were constructed and designated E. coli P8 and E. coli SS. E. coli P8 expressed a glycosyltransferase derived from Panax ginseng , whereas E. coli SS expressed a sucrose synthase derived from Arabidopsis thaliana . In the coupled system, sucrose synthase used sucrose and UDP to regenerate UDP-glucose (UDPG) in situ, while the glycosyltransferase catalyzed the conversion of Reb A into Reb D. A deep eutectic solvent (DES) composed of choline chloride and glycerol at a ChCl:Gly molar ratio of 1:2 was mixed with water to form a DES–H 2 O reaction medium. Under the selected conditions of pH 7.5, 45 °C, and a DES-to-water volume ratio of 40:60, P8 whole cells and an SS crude lysate converted 19.3 g/L Reb A into 21.0 g/L Reb D within 8 h using 500 mmol/L sucrose and 10 mmol/L UDP as co-substrates, corresponding to a molar yield of 93%. This study demonstrates an efficient and potentially environmentally friendly strategy for producing Reb D and other steviol glycosides. Download: Download high-res image (402KB) Download: Download full-size image
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JOURNAL OF CHROMATOGRAPHY A
·
2025
·
IF 4
Yan Zhang,Yaqian Zhou,Ping Liu,Yufeng Zhang,Yiqun Wan,Fan Liu,Genlin Sun,Lihua Chen,Jun Wang
A rapid and efficient method is highly desired for the determination of phenylurea herbicides (PUHs) in edible and medicinal herbs. Herein, we report a dispersive solid-phase extraction (d-SPE) method using a metal-organic framework Zn 4 O(NTB) 2 as the adsorbent. The microporous Zn 4 O(NTB) 2 can selectively extract targets while excluding matrix substances. Under optimized conditions, this method exhibits high sensitivity, with a limit of detections (LODs) and limits of quantification (LOQs) in the range of 0.09–0.32 ng mL -1 and 0.32–1.08 ng mL -1 , respectively. Moreover, the recoveries reach the range of 69.8 %-83.3 % with relative standard deviations below 6.2 %. The optimized adsorption efficiency of PUHs can be rapidly achieved in a short time of 10 min. Additionally, the adsorbent can be facilely regenerated with methanol washing. Furthermore, computational simulations reveal the guest-host interactions involving hydrogen bonding, π-π stacking interactions, and Van der Waals interactions. Practically, the established method has been successfully validated for extracting PUHs from Dendrobium candidum and Ganoderma lucidum samples.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2025
·
IF 6.2
Jing Gao,Hao Dong,Peichao Zhang,Kunpeng Gao,Haipeng Su,Jianan Sun,Xiangzhao Mao
Whole-enzyme cascade degradation of crystalline α-chitin is a long-term pursuit, which has been hampered by low transformation efficiency and heterogeneous products. Here, a novel β-N-acetylglucosaminidase (NAGase) from Sulfuriroseicoccus oceanibius, designated Sohex, was biochemically characterized. The enzyme demonstrated high hydrolytic activity toward N-acetyl chitooligomers (N-acetyl COSs) and exhibited promotive effects on crystalline α-chitin degradation. Furthermore, a whole-enzyme cascade degradation system based on lytic polysaccharide monooxygenases (LPMOs), chitinases, and Sohex was developed for N-acetyl-D-glucosamine (GlcNAc) production. This system achieved a maximum conversion rate of 45.93%, with GlcNAc purity reaching 99.99%. The addition of Sohex significantly enhanced α-chitin degradation efficiency, achieving conversion rates of 1.37-fold and 2.46-fold compared to the LPMOs + chitinases system and the sole chitinases system, respectively. Moreover, the promotion mechanisms of Sohex in conjunction with LPMOs and chitinases were elucidated, making the cascade system a promising strategy for converting crystalline α-chitin into high-purity GlcNAc.
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ACS Sustainable Chemistry & Engineering
·
2014
·
IF 0
Rui-Ying Bao,Wei Yang,Xin-Feng Wei,Bang-Hu Xie,Ming-Bo Yang
Melt processing of polymers is preferable in industry. Unfortunately, for high molecular weight (Mw) poly(l-lactide)/poly(d-lactide) (PLLA/PDLA) blends, exclusive formation of stereocomplex (sc) crystallites from the melt has never been achieved. We proposed a new and simple approach to enhance the melt crystallization of sc crystallites from high Mw PLLA/PDLA by using poly(ethylene glycol) (PEG). The crystallizability of the PLLA/PDLA blend is greatly enhanced by PEG during crystallization from the melt. The resulted crystalline structures depend on the Mw and content of PEG, and the crystallinity of sc crystallites increases with increasing content or decreasing Mw of PEG. More importantly, exclusive formation of sc crystallites is achieved in the blends with 10% PEG having Mw values of 1000 or 2000 g mol–1. Polarized optical microscopy (POM) observation shows that the spherulitic growth rates of sc crystallites are accelerated. The results demonstrate that segmental mobility of polylactides (PLA) chains plays a dominant role on the formation of sc crystallites from melt and provide a simple way to prepare sc crystallites from high Mw PLA by melt blending and enlarge the applications of PLA.
研究领域:
高分子科学
·
材料工程
·
聚合物结晶
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JOURNAL OF APPLIED POLYMER SCIENCE
·
2022
·Vol. 139
·期 22
·52268
·
IF 3.057
Zhixiang Cui,Yue Fan,Xiaolong Liu,Junhui Si,Qianting Wang
Nowadays, the environmental problems caused by industrial oily wastewater and offshore oil leakage are becoming more and more serious. Here, the 3-D porous polyurethane/deacetylated acetate cellulose (TPU/d-CA) composite foam with outstanding mechanical property and oil/water separation performance was successfully prepared. The morphology, wettability, mechanical properties, and oil/water separation performances had been systematically studied. The results showed that TPU/d-CA composite foam could be used as water and oil removing material, respectively, for a series of oil/water mixture only under gravity force, due to its lipophilicity and hydrophilicity. It has high-separation flux, which can reach up to 43,000 L/m 2 ·h and 85,000 L/m 2 ·h for water and oil, respectively and all the separation efficiency exceeds 98%. It also can be used as adsorbent material to absorb various oils, and the absorption capacity for carbon tetrachloride reached up to 34 g/g. Moreover, it can selectively, rapidly, and continuously separate oil or water by assisted peristaltic pump. After 15 cycle times, its separation efficiency is still as high as 98%. It has excellent comprehensive mechanical properties and antifouling ability. These results indicated that 3-D TPU/d-CA composite foam has broadly application prospects in oil/water separation field.
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