PLANT PHYSIOLOGY AND BIOCHEMISTRY
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2023
·
IF 6.5
Minglei Tao,Shengzhi Liu,Yaohan Li,Amin Liu,Jingkui Tian,Yuchang Liu,Hongwei Fu,Wei Zhu
Coumarin is an important secondary metabolite that affects plant physiology. It is a lactone of cis- o -hydroxycinnamic acid and widely exists in medicinal plants . Clematis terniflora DC. is a plant belonging to Ranunculaceae and is rich in variety of coumarins. Feruloyl-CoA 6′-hydroxylase has been reported as a key enzyme in the formation of coumarin basic skeleton only in some common plants, however, its evidence in other species is still lacking especially for the biosynthesis of coumarins in C . terniflora . In the present study, we identified a feruloyl-CoA 6′-hydroxylase CtF6′H in C . terniflora , and functional characterization indicated that CtF6′H could hydroxylate feruloyl-CoA to 6-hydroxyferuloyl-CoA. Furthermore, the expression level of CtF6′H was differed among different tissues in C. terniflora , while under UV-B radiation, the level of CtF6′H was increased in the leaves. Biochemical characteristics and subcellular location showed that CtF6′H was mainly present in the cytosol. The crystal structure of CtF6′H was simulated by homology modeling to predict the potential residues affecting enzyme activity . This study provides the additional evidence of feruloyl-CoA 6′-hydroxylase in different plant species and enriches our understanding of biosynthetic mechanism of coumarin in C . terniflora .
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Rice Science
·
2023
·
IF 4.8
Yang Xiaozheng,Liu Yusong,Huang Jing,Tao Ye,Wang Yifeng,Shen Renfang,Zhu Xiaofang
Phosphorus (P) starvation in rice facilitates the reutilization of root cell wall P by enhancing the pectin content. NaCl modulates pectin content, however, it is still unknown whether NaCl is also involved in the process of pectin regulated cell wall P remobilization in rice under P starved conditions. In this study, we found that 10 mmol/L NaCl increased the shoot and root biomasses under P deficiency to a remarkable extent, in company with the elevated shoot and root soluble P contents in rice. Further analysis indicated that exogenous NaCl enhanced the root cell wall P mobilization by increasing the pectin methylesterase activity and uronic acid content in pectin suggesting the involvement of NaCl in the process of cell wall P reutilization in P starved rice roots. Additionally, exogenous NaCl up-regulated the expression of P transporter OsPT6, which was induced by P deficiency, suggesting that NaCl also facilitated the P translocation prominently from root to shoot in P starved rice. Moreover, exogenous abscisic acid (ABA) can reverse the NaCl-mediated mitigation under P deficiency, indicating the involvement of ABA in the NaCl regulated root cell wall P reutilization. Taken together, our results demonstrated that NaCl can activate the reutilization of root cell wall P in P starved rice, which is dependent on the ABA accumulation pathway.
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CHEMICAL ENGINEERING JOURNAL
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2022
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IF 16.744
Jiejun Ren,Xiaopeng Zhou,Yuhua Wang
Metal halide perovskite quantum dots (PQDs) are considered as promising candidate for the next generation optoelectronic applications. However, their development is severely restricted by their poor stability. Here, we demonstrate the in situ growth of PQDs in the ordered channels of functionalized covalent-organic frameworks (COFs). The strong S-Pb chemical binding firmly anchors the PQDs in COF matrix, which prevent the aggregation of PQDs and eliminate the nonradiative trap centers of PQDs, leading to the high photoluminescence quantum yield (81.5 %) and remarkable stability. Femtosecond transient absorption spectroscopy reveal the elimination of nonradiative trap centers in PQD@COFs. Theoretical simulation results demonstrate the strong waveguide effect in PQD@COFs, which reduces the reabsorption loss. Further, the obtained white light-emitting diode exhibits ultrahigh luminous efficiency of 89.6 lm/W and excellent operating stability, which paves the way for the commercial application of PQDs.
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FOOD CHEMISTRY
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2022
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IF 9.231
Yat-Tin Chan,Jiating Huang,Ho-Ching Wong,Jun Li,Danyue Zhao
Polyphenols, ubiquitous in plant-originated foods, exhibit multifarious health benefits but are notorious for their low bioavailability. As the majority of polyphenols enter the colon where they are metabolized into the more bioavailable metabolites with multifarious bioactivities, gut microbiota composition is critical for realizing the health benefits of polyphenols. Black raspberries (BRB) are highly abundant in health-promoting but poorly absorbable polyphenols, primarily ellagitannins and anthocyanins. This study aimed to dissect the bilateral interactions between polyphenols and gut microbiotas of diverse origins. Using an optimized extraction procedure, BRB polyphenols were first comprehensively characterized by UHPLC-QTOF-MS/MS. Next, in vitro anaerobic fermentations with microbiota from human adults, infants, rats, and mice were conducted for comparison of phenolic profile (by metabolomics) and microbiota composition (by 16S rDNA sequencing) before and after fermentation. Bioinformatics analysis further revealed unique polyphenol-microbiota interactions and identified several bacterial species that could potentially produce bioactive and bioavailable phenolic metabolites following BRB consumption.
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Chinese Medicine
·
2022
·Vol. 17
·期 1
·1-15
·
IF 4.546
Zhang Shiyu,Mi Yinxiao,Ye Tingting,Lu Xiaoyan,Liu Li,Qian Jing,Fan Xiaohui
Background Shenmai injection (SMI), a traditional Chinese medicine (TCM) injection prepared from Red ginseng and Ophiopogon japonicus, is widely used in clinics to treat chemotherapy-induced myelosuppression. Similar to other TCM injections, SMI contains a high amount of carbohydrates (fructose, sucrose, and maltose) in addition to the bioactive substances, specifically ginsenosides (Rg1, Re, and Rb1). To date, the role of these carbohydrates in the hematopoietic function of SMI remains unclear.PurposeWe aimed to investigate the hematopoietic effects and potential mechanisms of SMI and its components, focusing on the carbohydrates present in SMI.Experimental design/methodsFirst, we evaluated the hematopoietic effect of SMI on 5-fluorouracil (5-FU)-induced myelotoxicity in a tumor-bearing mouse model. Then we prepared mixtures of ginsenosides and carbohydrates according to their proportions in SMI and evaluated their hematopoietic function in mice with 5-FU-induced myelosuppression. Finally, hematopoiesis-related molecular networks were built based on RNA sequencing (RNA-seq) of the bone marrow stromal cells (BMSCs), and the potential mechanisms of carbohydrates and ginsenosides were evaluated.Results SMI attenuated 5-FU-induced myelotoxicity in tumor-bearing mice. Both ginsenosides and carbohydrates increased the bone marrow nucleated cell (BMNC) count and improved the bone marrow morphology in myelosuppressive mice; they promoted the proliferation of BMSCs derived from those myelosuppressive mice. Bioinformatics analyses revealed ECM-receptor interaction, Hippo signaling, and Wnt signaling are common pathways regulated by both ginsenosides and carbohydrates; Gstt1, Gstp2, Gsta4 and Oplah in Glutathione metabolism pathway and Cd19, Cd79a, and Cd79b in B cell receptor pathway are uniquely regulated genes related to carbohydrates but not ginsenosides.Conclusion sCarbohydrates may collaborate with ginsenosides and contribute to the hematopoietic function of SMI. Carbohydrates could be considered as a bioactive component in this TCM injection.Graphical Abstract
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APPLIED SURFACE SCIENCE
·
2022
·
IF 7.392
Shuying Gao,Lingling Pan,Weiguo Kong,Li Zhou,Jian Wu,Shijian Zhou,Weilong Shi,Fu Yang,Yan Kong
In this study, an energetic materials (EM) deflagration process coupled with a gas-shocking exfoliation strategy was proposed for the in-situ introduction of a π-electron-conjugated structure into ultrathin g-C 3 N 4 nanosheets. The optimal 2C-CNS sample was endowed with a porous-ultrathin-nanosheets morphology (3–4 nm thickness), larger exposed surface area (144.1 m 2 /g), and significant pore volume (0.51 cm 3 /g). Using urotropin-deflagration and gas-shocking-driven processes, the precursors were dissociated and copolymerized in an aqueous solution into g-C 3 N 4 nanosheets chemically modified with conjugated carbon moieties. Most importantly, an expanded π-electron conjugated system was successfully constructed between the carbon moieties that interacted with the ultrathin g-C 3 N 4 (2C-CNS), which endowed its with a faster electron-hole separation efficiency and enhanced visible-light harvesting ability. The H 2 evolution rate and Cr 6+ reduction efficiency for 2C-CNS reached 2360 μmol h −1 g −1 for k = 0.0301 min −1 , which was almost 25.8/23 times higher than that of bulk g-C 3 N 4 (Bu-CN, 91.2 μmol h −1 g −1 , k = 0.0013 min −1 ). Also, 2C-CNS showed 2.8/2.4 times higher performance than the g-C 3 N 4 ultrathin nanosheets without a carbon-section modification (0C-CNS, 853 μmol h −1 g −1 , k = 0.0125 min −1 ).
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ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2023
·Vol. 30
·期 16
·48248-48259
·
IF 5.8
Pan Zewen,Tang Changyuan,Cao Yingjie,Xuan Yingxue,Zhou Qianyi
The sources and distribution characteristics of three phenolic endocrine-disrupting compounds (EDCs), e.g., alkylphenols (APs) (including nonylphenols (NPs) and 4-t-octylphenol (OP)) and Bisphenol A (BPA), were investigated in the rivers of the Pearl River Delta Region (PRDR) with complex land-use types. The mean concentrations of NPs, OP, and BPA in river water including wet and dry seasons were 87, 6, and 74 ng/L in the agricultural regions ( n = 10), 135, 7, and 61 ng/L in the transitional regions ( n = 8), and 249, 15, and 152 ng/L in the urban regions ( n = 28). Contents of NPs and BPA were high in the river sediments (ranged from 7 to 3048 ng/g and 2 to 271 ng/g, respectively). Equilibrium analysis results suggested that sediment release was not the main source of the river EDCs. Principal component analysis (PCA) showed that sewage was the major source of EDCs in the dry season, while the leaching effect of rainfall on the agricultural soils, urban roads, and commercial products was an important source in the wet season. Furthermore, the ratio of APs and total concentration of phenolic EDCs (ΣEDCs) was used to characterize the agricultural regions and urban regions in the PRDR. The ratio was less than 0.6 in the agricultural regions while the ratio was large than 0.6 in the dry season and less than 0.6 in the wet season in urban regions. BPA and NPs in transitional region and urban region had small/medium potential risk to aquatic organisms.
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MICROPOROUS AND MESOPOROUS MATERIALS
·
2022
·
IF 5.876
Dan Li,Lijun Guo,Feng Li,Jin Huang,Jiahui Li,Miao Li,Cuiqin Li
Two covalent organic frameworks (COFs), as the supports of transition metal catalysts, were prepared and coordinated with nickel to synthesize two precatalysts for ethylene oligomerization . The chemical structure of sub-constructing unit had a significant influence on the specific surface area and the catalytic performance of covalent organic frameworks supported nickel(Ⅱ) catalysts. Two COFs were stacked in lamellae, and the specific surface area and the pore volume of COF with p -phthalaldehyde as sub-constructing unit were far larger than those of COF with butanedione as sub-constructing unit. The catalyst with a larger specific surface area had a higher catalytic activity and a higher selectivity of low carbon olefins in ethylene oligomerization because of a higher specific surface areas and larger pore structure for ethylene diffusion and mass transfer. Two catalysts can be reused at least three times without considerable loss in the catalytic activity and the selectivity toward short-chain α-olefins.
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ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2022
·Vol. 29
·期 20
·30337-30347
·
IF 5.19
Yang Shuyi,Zou Hongtao,Cheng Ying,Tao E.
Owing to containing high fraction of organic matter, the tannery sludge seemed to be fit for composting. Actually, it was intensively harmful to the environment, due to containing chromium (Cr). So it might undergo a long time of storage until finding a proper way to dispose it. In the storage period, it would expose the surrounding environment a risk via releasing Cr. In this study, an approach was proposed to minimize the amount of released Cr, and reveal the mechanism on immobilizing Cr. Collagen protein waste (CPW) was adopted to immobilize Cr, and it was evaluated via leaching experiment. The lowest leaching concentration of Cr was 12 mg/L, meeting the limits of related standard in China (GB 5085.3–2007, Tcr < 15 mg/L). Moreover, the compositions and functional groups of the optimum sample (12 mg/L) were also characterized, confirming that the dominant functional groups cross-linking with Cr were hydroxyl (-OH), carboxyl (-COOH), and epoxy (-COC). Importantly, density functional theory (DFT) calculation was also employed, suggesting that Cr was restrained by accepting electrons from O atoms donating by functional groups.
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FLUID PHASE EQUILIBRIA
·
2022
·
IF 2.745
Yingying Huang,Chuanjun Wu,Ying Chen,I-Ming Chou,Lei Jiang
Diffusion coefficients of hydrogen sulfide in water and brine are critical in geology and chemical industries for hydrogen sulfide geological storage and hydrogen sulfide separation from natural gas. However, previous studies have mostly measured the diffusion coefficient of hydrogen sulfide in water at room temperature and normal pressure and the diffusion coefficient of hydrogen sulfide in brine had not been reported. In this study, ratio of the Raman signal intensity of the H-S bond of hydrogen sulfide to the H O bond of water, H R H 2 S / H 2 O , was used to measure the diffusion coefficients of hydrogen sulfide in water at 0.1–2 MPa and 273.1–373.1 K and in four brine solutions (0.62, 1, 2, and 3 mol•kg −1 NaCl) at 1 MPa and 273.1–373.1 K. Results showed that the diffusion coefficient of hydrogen sulfide in water increased with the increasing temperature and was insignificantly affected by the pressure, which follows the relationship derived from the Speedy–Angell power-law: D ( c m 2 / s ) = 13.013 × 10 − 5 [ T / 216.478 − 1 ] 1.974 , where T is in the temperature (K). In brine, the diffusion coefficient of hydrogen sulfide also increased with the temperature and was clearly inhibited by salinity, which can be described using the Ratcliff–Holdcroft model D ( c m 2 / s ) = 13.013 × 10 − 5 ( T / 216.478 ) 1.974 ( 1 − 0.0755 C s ) , where Cs is concentration of NaCl in brine (mol•kg −1 ).
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MOLECULES
·
2022
·Vol. 27
·期 3
·866
·
IF 4.927
Wei Du,Hong-Min Fan,Yu-Xin Zhang,Xiao-Hua Jiang,Yun Li
Background: Excessive oxidative stress is associated with hypertension in professional high-temperature working conditions. Polyphenols exhibit a cardioprotective effect. Hawthorn contains high amounts of flavonoids, though its effect on hypertension protection has yet to be studied. This study aims to investigate this effect of extract of hawthorn (EH) or its combination with vitamin C (Vit. C) in rats induced by working under a hot environment. Methods: Forty-two male rats were randomly divided into a control group under normal temperature and six treatment groups exposed at 33 ± 1 °C along with 1 h of daily treadmill running. They were orally provided with water, Vit. C (14mg/kg), EH (125, 250, and 500 mg/kg), and EH500 + Vit. C, once a day for four weeks. Results: Both EH and Vit. C alone reduced the systolic and diastolic blood pressure of rats exposed to the heat environment; however, their joint supplementation completely maintained their blood pressure to the normal level throughout the experimental period. No morphological changes were found on the intima of aorta. Moreover, the co-supplementation of EH and Vit. C prevented the changes of heat exposure in inducing oxidative stress markers, such as glutathione peroxidase, catalase, total antioxidant capacity, and nitric oxide; the synergistic action was more effective than either individual treatment of EH and Vit. C. Furthermore, the administration of EH had more potent effects on increasing superoxide dismutase, IL-2, the 70 kilodalton heat shock proteins and high sensitivity C reactive protein, and decreasing serum malondialdehyde and lipofuscin in vascular tissue than those in Vit. C group. Conclusions: A strong synergistic effect of EH and Vit. C on the prevention of hypertension under heat exposure was established, as they inhibited the oxidative stress state. This study also sets up a novel intervention strategy in animal models for investigation on the early phases of hypertension induced by heat exposure.Keywords:hypertension;oxidative stress;heat exposure;hawthorn flavonoids;vitamin C;physical effort;rats
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Polymers
·
2022
·Vol. 14
·期 3
·433
·
IF 4.967
Chuanbo Liu,Shutian Liu,Conglin Dong,Chengqing Yuan,Xiuqin Bai
Reducing the deformation of polymer matrix materials can decrease the fluctuation of coefficient of friction (COF), and friction-induced vibration and its amplitudes. HDPE composites with T-ZnOw as a fixed strengthening filler were modified with the addition of Si3N4particles at different concentrations. The COFs, wear rates, micro-morphologies, and friction-induced vibrations were obtained by conducting sliding tests against carbon steel balls in an aqueous environment at a low velocity and high load. The mechanism of the reduction in frictional fluctuation due to the addition of Si3N4particles was revealed through the frictional responses. The results demonstrated that 4 wt% addition of Si3N4in HDPE can enhance the strain–stress property and improve the lubrication by forming a lubricating film. Therefore, the surface deformation and the fluctuations of COFs and its vibrations were reduced. The aggregation phenomenon and reduced strain–stress response at a high concentration of Si3N4disrupted the positive fluctuating reduction, and resulted in a rough surface with severe tearing and cracking deformations. Additionally, it led to fluctuating wear behaviors with high COF and vibrations. The results obtained in this study can elucidate the effects of adding Si3N4particles to enhance lubrication in polymer composites. Additionally, the results provide a new research method for designing and manufacturing polymer-based composites with low friction-induced fluctuations.Keywords:HDPE composite;Si3N4;surface deformation;fluctuation reduction
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