INORGANIC CHEMISTRY
·
2023
·
IF 4.6
Xiaoli Yang,Hongmin Duan,Ruifeng Wang,Fengwang Zhao,Fayi Jin,Wei Jiang,Guangting Han,Qingxin Guan,Haoxi Ben
The utilization of Cu-based catalysts in CO2 conversion into valuable chemicals is of significant interest due to their potential in mitigating greenhouse gas emissions. However, the controllable design of Cu-based catalysts and the regulation of their mechanism remain challenging. In this study, a series of efficient Cu/L catalysts were prepared for this process, and the intrinsic influencing factors on the reaction routes were systematically revealed. Various techniques revealed that Cu particles in L-supported catalysts exhibited higher dispersion and formed Cu–O(OH)–K interfacial sites. However, with increasing Cu loading, the dispersion of Cu particles and the percentage of Cu–O(OH)–K interfaces decreased. Kinetic investigations revealed that the adsorption configuration and electronic structure of Cu species codetermined the reaction pathways and resulting selectivity. Cu/L catalysts possessing Cu–O(OH)–K interfaces and small particles demonstrated the preferential formation of formate species, promoting methanol formation. However, larger Cu particles generated carboxylate intermediates, resulting in higher CO selectivity..
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Frontiers in Plant Science
·
2022
·
IF 6.627
Tan Zhanming,Wu Cuiyun,Xuan Zhengying,Cheng Yunxia,Xiong Renci,Su Zhihang,Wang Desheng
Lead (Pb) is a heavy metal pollutant and negatively affects agriculture and ecosystems. Pb can cause oxidative stress and abnormal plant growth. The ascorbic acid-glutathione (AsA-GSH) cycle mainly exists in chloroplasts and resists oxidative stress, scavenges reactive oxygen radicals, and maintains normal photosynthesis. However, the dosage related effects of Pb on pakchoi photosynthesis, via oxidative stress and the AsA-GSH system, remains unclear. In this study, various Pb dosage stress models were tested (low: 300 mg/kg; medium: 600 mg/kg; high: 900 mg/kg). Pb stress induced a dose-dependent increase in Pb content in pakchoi leaves (P < 0.05). Principal component analysis showed that Se, B, and Pb were significantly and negatively correlated. Pb stress also increased MDA content and decreased antioxidant enzymes SOD, GSH-Px, and T-AOC activities (P < 0.05). We also found that Vc content, as well as the GSH/GSSG ratio, decreased. Additionally, Pb stress destroyed chloroplast structure, decreased photosynthesis indicators Pn, Tr, Gs, Ci and VPD, and attenuated Fv/Fm and Fv/Fo (P < 0.05). In the high-dose group, the contents of chlorophyll a, chlorophyll b, and carotenoids decreased significantly, while the expression of chloroplast development genes (GLK, GLN2) decreased (P < 0.05). Our data suggest that Pb stress leads to dosage-dependent, aberrant photosynthesis by inhibiting the AsA-GSH system in pakchoi. This study expands the Pb toxicology research field and provides indications for screening antagonists.
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Nutrients
·
2022
·Vol. 14
·期 21
·4549
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IF 6.706
Tongtong Ba,Dai Zhao,Yiqin Chen,Cuiping Zeng,Cheng Zhang,Sai Niu,Hanchuan Dai
L-citrulline (L-cit) is a key intermediate in the urea cycle and is known to possess antioxidant and anti-inflammation characteristics. However, the role of L-cit in ameliorating oxidative damage and immune dysfunction against iron overload in the thymus remains unclear. This study explored the underlying mechanism of the antioxidant and anti-inflammation qualities of L-cit on iron overload induced in the thymus. We reported that L-cit administration could robustly alleviate thymus histological damage and reduce iron deposition, as evidenced by the elevation of the CD8+T lymphocyte number and antioxidative capacity. Moreover, the NF-κB pathway, NCOA4-mediated ferritinophagy, and ferroptosis were attenuated. We further demonstrated that L-cit supplementation significantly elevated the mTEC1 cells’ viability and reversed LDH activity, iron levels, and lipid peroxidation caused by FAC. Importantly, NCOA4 knockdown could reduce the intracellular cytoplasmic ROS, which probably relied on the Nfr2 activation. The results subsequently indicated that NCOA4-mediated ferritinophagy was required for ferroptosis by showing that NCOA4 knockdown reduced ferroptosis and lipid ROS, accompanied with mitochondrial membrane potential elevation. Intriguingly, L-cit treatment significantly inhibited the NF-κB pathway, which might depend on restraining ferritinophagy-mediated ferroptosis. Overall, this study indicated that L-cit might target ferritinophagy-mediated ferroptosis to exert antioxidant and anti-inflammation capacities, which could be a therapeutic strategy against iron overload-induced thymus oxidative damage and immune dysfunction.Keywords:L-citrulline;thymus;iron overload;ferritinophagy;ferroptosis
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BIOMEDICAL CHROMATOGRAPHY
·
2022
·Vol. 37
·期 2
·e5536
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IF 1.911
Qi Dong,Na Hu,Huilan Yue,Honglun Wang,Yue Wei
α -glucosidase inhibitors (AGIs) are widely used for the treatment of type 2 diabetes, but their side effects have made it to develop novel and alternative AGIs immediately. In this study, the extract of Hypericum perforatum L. (HPE) has been confirmed to have α -glucosidase inhibitory activity in vitro and in vivo. Seven active compounds, rutin, hyperoside, isoquercitrin, avicularin, quercitrin, quercetin, and biapigenin, were screened based on a bio-affinity chromatography column with α -glucosidase enzyme-conjugated solid phase and UPLC/MS, which exhibited excellent α -glycosidase inhibitory effects by the determined IC 50 values. The mechanism of α -glycosidase inhibitory activity of biapigenin was studied for the first time. The results showed that biapigenin was a high-potential, reversible, and mixed enzyme inhibitor. Analysis by molecular docking further revealed that hydrophobic interactions were generated by interactions between biapigenin and amino acid residues LYS156, PHE303, PHE314, and LEU313. In addition, hydrogen bonding occurred between biapigenin and α -glucosidase amino acid residues ASP307, SER241, and LYS156. This research identified that biapigenin could be a novel AGI and further applied to the development of potential anti-diabetic drugs. Furthermore, our studies established a rapid in vitro screening method for AGIs from plants.
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JOURNAL OF ELECTRONIC MATERIALS
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2022
·Vol. 52
·期 1
·613-627
·
IF 2.047
Huang Jianfeng,Cai Zhengyu,Zhang Yong,Pei Lizhai
BiPr oxide nanoparticles were successfully synthesized via a facile surfactant-free hydrothermal route using sodium bismuthate and praseodymium nitrate, and polyaniline/BiPr oxide nanoparticles were also obtained. The products were investigated by x-ray diffraction (XRD), scanning electron microscopy (SEM) and transmission electron microscopy. The obtained nanoparticles with a diameter of about 50–200 nm are composed of polycrystalline structures with rhombohedral Bi 0.4 Pr 0.6 O 1.5 , monoclinic Bi 2 O 3 and monoclinic Pr 5 O 9 phases. Irregular nanoscale polyaniline particles cover the surface of the BiPr oxide. A pair of quasi-reversible cyclic voltammetry (CV) peaks are located at −0.04 V and −0.67 V, respectively, at the BiPr oxide nanoparticle-modified glassy carbon electrode (GCE) in 0.1 M KCl solution with 2 mM l -cysteine. The anodic CV peak shifts positively to +0.14 V and the cathodic CV peak shifts negatively to −0.82 V using the polyaniline/BiPr oxide nanoparticle-modified GCE. The linear range and detection limit are 0.005–2 mM and 1.18 μM, 0.0005–2 mM and 0.16 μM for the GCE modified with BiPr oxide nanoparticles and polyaniline composites, respectively. Polyaniline greatly enhances the electrochemical sensing properties of the BiPr oxide nanoparticle-modified GCE. Graphical Abstract
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2022
·
IF 5.895
Huibing Chi,Yilian Wang,Bingjie Xia,Yawen Zhou,Zhaoxin Lu,Fengxia Lu,Ping Zhu
l-Asparaginase has gained much attention for effectively treating acute lymphoblastic leukemia (ALL) and mitigating carcinogenic acrylamide in fried foods. Due to high-dose dependence for clinical treatment and low mitigation efficiency for thermal food processes caused by poor thermal stability, a method to achieve thermostable l-asparaginase has become a critical bottleneck. In this study, a rational design including free energy combined with structural and conservative analyses was applied to engineer the thermostability of l-asparaginase from Bacillus licheniformis (BlAsnase). Two enhanced thermostability mutants D172W and E207A were screened out by site-directed saturation mutagenesis. The double mutant D172W/E207A exhibited highly remarkable thermostability with a 65.8-fold longer half-life at 55 °C and 5 °C higher optimum reaction temperature and melting temperature (Tm) than those of wild-type BlAsnase. Further, secondary structure, sequence, molecular dynamics (MD), and 3D-structure analysis revealed that the excellent thermostability of the mutant D172W/E207A was on account of increased hydrophobicity and decreased flexibility, highly rigid structure, hydrophobic interactions, and favorable electrostatic potential. As the first report of rationally designing l-asparaginase with improved thermostability from B. licheniformis, this study offers a facile and efficient process to improve the thermostability of l-asparaginase for industrial applications.
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Polymer-Plastics Technology and Materials
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2022
·
IF 2.439
Wenqiang Wang,Yiming Gong,Qingxia Huang,Liuzemu Li,Airong Xu,Rukuan Liu
Green polyvinyl alcohol/poly(D,L-lactide-co-glycolide) (PVA/PLGA) composite materials were developed by dissolving PVA and PLGA in hexafluoroisopropanol (HFIP), followed by the subsequent layout of PVA/PLGA/HFIP liquid film and the removal of HFIP. Interestingly, the PVA/PLGA composite materials display remarkedly enhanced tensile strength, elongation at break, biodegradability and biocompatibility. Systematic investigations have been completed to reveal the effects of PVA/PLGA mass ratio on the tensile strength, elongation at break, biodegradability and biocompatibility. Meanwhile, the morphology, crystallinity and chemical structure of the PVA/PLGA material and possible interaction between PVA and PLGA are also investigated using scanning electron micrograph, infrared spectra and X-ray diffraction techniques.
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JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2022
·
IF 9.965
Yuming Xie,Jingqiu Li,Mengjue Cao,Yi Feng,Jianfeng Yao
CoS 2 /Co-Ni layered double hydroxides (LDHs) with a rational tailored pore structure are developed by partial sulfurization of Co-ZIF-L and subsequent Ni 2+ etching for the transformation of zeolitic imidazolate frameworks (ZIFs) to layered double hydroxides . The as-synthesized CoS 2 /Co-Ni LDHs maintain the leaf-like structure and have rich hierarchical pores. More importantly, CoS 2 nanoparticles are uniformly embedded among Co-Ni LDH layers, facilitating mass diffusion and favorably exposing more active sites. Benefiting from these desirable compositional and structural features, a remarkable specific capacitance (capacity) of 1784 F/g (223 mAh/g) at 1 A/g is delivered by CoS 2 /Co-Ni LDHs. Furthermore, the as-assembled CoS 2 /Co-Ni LDH//activated carbon as the asymmetric supercapacitor device also exhibits a high energy density of 125 Wh/kg at a power density of 800 W/kg and a superb capacitance retention of 98 % after 5000 cycles, outperforming many Co-Ni LDH based electrode materials reported in the literature.
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DRYING TECHNOLOGY
·
2022
·
IF 3.556
Yanzhen Long,Min Zhang,Arun S. Mujumdar,Jingjing Chen
Turmeric has high commercial demand internationally, owing to its high nutrient and medical value. In this research, turmeric samples were subjected to several drying methods viz. infrared freeze drying (IRFD), freeze drying (FD), and pulse-spouted microwave-assisted freeze-drying (PSMFD). Over the range of operating conditions employed in this study, IRFD of infrared radiation was showed to shorten the drying time by 21.27% relative to FD, while the retention valves of ascorbic acid, curcumin, total phenols, and total flavonoids remains at 75.61%, 94.06%, 69.00%, and 80.09%, respectively over the range of experimental parameters. IRFD significantly (P < 0.05) reduced 25.19% of the energy consumption in comparison with FD. Furthermore, the interior of IRFD samples exhibited a honeycomb porous structure, and increased hardness, lower shrinkage, apparent density compared to PSMFD. Additionally, IRFD also performed better than PSMFD and FD under vacuum freezing conditions.
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INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2022
·Vol. 23
·期 12
·6588
·
IF 6.208
Jiafeng Niu,Ruxue Yan,Juan Shen,Xiaoyu Zhu,Fanqiang Meng,Zhaoxin Lu,Fengxia Lu
Type I L-asparaginase fromBacillus licheniformisZ-1 (BlAase) was efficiently produced and secreted inBacillus subtilisRIK 1285, but its low yield made it unsuitable for industrial use. Thus, a combined method was used in this study to boost BlAase synthesis inB. subtilis. First, fifteen single strong promoters were chosen to replace the original promoter P43, with PyvyD achieving the greatest BlAase activity (436.28 U/mL). Second, dual-promoter systems were built using four promoters (PyvyD, P43, PaprE, and PspoVG) with relatively high BlAase expression levels to boost BlAase output, with the engine of promoter PaprE-PyvyD reaching 502.11 U/mL. The activity of BlAase was also increased (568.59 U/mL) by modifying key portions of the PaprE-PyvyD promoter. Third, when the ribosome binding site (RBS) sequence of promoter PyvyD was replaced, BlAase activity reached 790.1 U/mL, which was 2.27 times greater than the original promoter P43 strain. After 36 h of cultivation, the BlAase expression level in a 10 L fermenter reached 2163.09 U/mL, which was 6.2 times greater than the initial strain using promoter P43. Moreover, the application potential of BlAase on acrylamide migration in potato chips was evaluated. Results showed that 89.50% of acrylamide in fried potato chips could be removed when combined with blanching and BlAase treatment. These findings revealed that combining transcription and translation techniques are effective strategies to boost recombinant protein output, and BlAase can be a great candidate for controlling acrylamide in food processing.Keywords:L-asparaginase;Bacillus subtilis;promoter;dual-promoter systems;ribosome binding site;scale-up fermentation;acrylamide
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RESEARCH ON CHEMICAL INTERMEDIATES
·
2022
·Vol. 48
·期 10
·4287-4311
·
IF 3.134
Xu Yanming,Zhang Shuyuan,Zhao Yuhao,Yang Jingjing,Shen Shusu,Zhang Ganwei,Guo Yongfu,Zheng Xinyu
To enhance the adsorption capacity and improve agglomeration properties of kaolin during the adsorption process of Hg 2+ from aqueous solution, kaolin was bi-functionally modified using L-cysteine and polypyrrole. The as-synthetized adsorbent of L-Ppy-Fe 3 O 4 /kaolin was characterized before and after modification. The removal conditions were optimized by response surface methodology, and the optimized results show that the optimal adsorption capacity of Hg 2+ reaches 482.71 mg/g at pH of 7.9, temperature of 315 K, initial concentration of 40 mg/L and dosage of 0.05 g/L. And the effects of adsorption conditions on Hg 2+ removal follow the order of solution pH > temperature > initial concentration > dosage. The adsorption process of Hg 2+ in aqueous solution can be well described by pseudo-second-order kinetic model and Langmuir isothermal model. Moreover, the thermodynamic fitting indicates that the adsorption of Hg 2+ is an endothermic and spontaneous process. The adsorption mechanism of Hg 2+ onto L-Ppy-Fe 3 O 4 /kaolin is mainly the complexation between divalent Hg 2+ and sulfhydryl/amino groups to form stable metal complexes, and electrostatic attraction is also a part of the reaction mechanism. In addition, L-Ppy-Fe 3 O 4 /kaolin is proved to have satisfactory dispersion ability and chemical stability and can be easily separated and recovered after adsorption in aqueous solution. Therefore, L-Ppy-Fe 3 O 4 /kaolin is a promising adsorbent for the efficient removal of mercury ions. Graphical abstract
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Journal of Materials Chemistry B
·
2022
·Vol. 10
·期 27
·5191-5202
·
IF 7.571
Anning Li,Siqi Wang,Zhiqiang Zhang,Na Xu,Guixia Ling,Peng Zhang
Severe systemic toxicity and side effects are major obstacles to the success of chemotherapy for tumors. Regardless of the choice of chemotherapy drugs, the safety of drug delivery materials is crucial, and therefore, there have been various efforts to improve the therapeutic effect and the biological safety of drug delivery systems (DDSs). In this study, a dual stimulus-response DDS (PLL-SS@DOX-BP) was constructed based on the biomaterials of black phosphorus (BP) nanosheets and poly-l-lysine (PLL) to enhance the treatment of doxorubicin hydrochloride (DOX) for breast cancer. The PLL derivative was nano-coated on the surface of drug-loaded BP nanosheets, and it prevented premature leakage of the drug and maintained the stability of the DDS. The introduced disulfide bonds and photothermal agent BP enabled the redox and near-infrared responsive drug release of the DDS, and the coated PLL derivative on the nanocarrier decreased premature leakage of the drug before the DDS reached the tumor tissues. The in vitro and in vivo experiments showed that the combination of biomaterial (PLL) and photothermal material (BP nanosheets) exhibited excellent biological safety and remarkable drug delivery capacity. Moreover, the pharmacodynamic studies indicated that PLL-SS@DOX-BP is a powerful vehicle for photothermal therapy in combination with chemotherapy. Compared with chemotherapy alone, the developed DDS displayed enhanced anti-tumor efficiency with decreased systemic toxicity, and thus, it has the potential to be a promising anti-tumor treatment strategy.
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