MICROCHIMICA ACTA
·
2020
·Vol. 187
·期 2
·1-9
·
IF 6.232
Xiang Ling,Zhang Feng,Feng Jian,Chen Chunyan,Cai Changqun
A method is described for the simultaneous determination of hepatocellular carcinoma-associated microRNA-122 and microRNA-199a/b-3p. This probe consists of two kinds of nanomaterials. The first comprises CdTe@CdS core-shell quantum dots which, on excitation at 375 nm give two emissions, with peak wavelengths at 543 (g-QDs) and at 627 nm (r-QDs). The second comprises gold nanoparticles acting as a quencher. In the absence of the target, g-QD-N1 and r-QD-N2 are stable due to the fluorescence stability. With the addition of microRNA-122 and microRNA-199a/b-3p, g-QD-N1 and r-QD-N2 are conjugated to the surface of AuNP-S1/S2 through base complementary pairing. As a result, fluorescence resonance energy transfer (FRET) occurs, resulting in a decrease at 550 nm and 635 nm respectively, which can realize the simultaneous detection of two different microRNAs. Detection is achieved within 50 min. The detection limits (3σ/k) are 0.2 nM for microRNA-122 and 0.5 nM for microRNA-199a/b-3p. The clinical applicability of the assay was demonstrated by detecting microRNAs in human serum and different cell lysates.
Schematic for the simultaneous determination of microRNA-122 and microRNA-199a/b-3p by FRET.
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Nano Research
·
2020
·Vol. 13
·期 2
·511-526
·
IF 8.183
Zhu Zhou,Jiang Shaokang,Liu Yanhua,Gao Xiaomeng,Hu Shanshan,Zhang Xin,Huang Chao,Wan Qianbing,Wang Jian,Pei Xibo
In recent years, various particulate materials have played important roles in medical applications. However, nano- and micron-sized particles of the same material could exhibit distinct properties due to different particle sizes. This finding provided a simple and effective way to improve the biological applications of particulate materials. Therefore, as a highly promising member, the effect of the particle size change of the magnesium metal organic framework-74 (Mg-MOF74) was well worth evaluating. Here we firstly assessed the in vitro and in vivo toxicity of micron/nanoscale Mg-MOF74 (m-Mg-MOF74/n-Mg-MOF74) in detail. Our in vitro study revealed that compared to micron-sized subjects, n-Mg-MOF74 provided a wider range of safe concentrations. Furthermore, both micron/nanoscale Mg-MOF74 showed good biocompatibility and allowed all the rats under the treatment to survive through the expected experimental periods, with n-Mg-MOF74 still showing lower cardiotoxicity. These advantages of nanoscale Mg-MOF74 might benefit from its sustainable and balanced release of Mg 2+ both inside and outside the cells. Based on the biosafety evaluation, advanced bio-functional assessments of m/n-Mg-MOF74 including early osteogenesis and angiogenesis were also performed. Similarly, the suitable dose groups of n-Mg-MOF74 achieved optimal early osteogenic promotion and angiogenic stimulation effects. Overall, our combined data delineated the toxicity and biological behaviors of Mg-MOF74 of different scales, and suggested nanoscale Mg-MOF74 as a better choice for future applications. This result revealed that particle size reduction might be a viable strategy to improve and expand medical applications of MOFs or other particulate materials.
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International Journal of Minerals Metallurgy and Materials
·
2020
·Vol. 27
·期 1
·37-45
·
IF 1.713
Wang Yu,Zhang Guo-hua,Wu Yue-dong,He Xin-bo
The method of calciothermic reduction of B 4 C was proposed for preparing CaB 6 . The phase transition and morphology evolution during the reaction were investigated in detail. The experimental results reveal that Ca first reacts with B 4 C to generate CaB 2 C 2 and CaB 6 at a low temperature and that the CaB 2 C 2 subsequently reacts with Ca to produce CaB 6 and CaC 2 at a high temperature. After the products were leached to remove the byproduct CaC 2 , pure CaB 6 was obtained. The grain size of the prepared CaB 6 was 2–3 μm, whereas its particle size was 4–13 μm; it inherited the particle size of B4C. The residual C content of the product was decreased to 1.03wt% after the first reaction at 1173 K for 4 h and the second reaction at 1623 K for 4 h.
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Nature Communications
·
2021
·Vol. 12
·期 1
·1-12
·
IF 14.919
Zhao Yan,Zhang Song,Yu Tianhao,Zhang Yan,Ye Guo,Cui Han,He Chengzhi,Jiang Wenchao,Zhai Yu,Lu Chunming,Gu Xiaodan,Liu Nan
Accurate and imperceptible monitoring of electrophysiological signals is of primary importance for wearable healthcare. Stiff and bulky pregelled electrodes are now commonly used in clinical diagnosis, causing severe discomfort to users for long-time using as well as artifact signals in motion. Here, we report a ~100 nm ultra-thin dry epidermal electrode that is able to conformably adhere to skin and accurately measure electrophysiological signals. It showed low sheet resistance (~24 Ω/sq, 4142 S/cm), high transparency, and mechano-electrical stability. The enhanced optoelectronic performance was due to the synergistic effect between graphene and poly (3,4-ethylenedioxythiophene) polystyrene sulfonate (PEDOT:PSS), which induced a high degree of molecular ordering on PEDOT and charge transfer on graphene by strong π-π interaction. Together with ultra-thin nature, this dry epidermal electrode is able to accurately monitor electrophysiological signals such as facial skin and brain activity with low-motion artifact, enabling human-machine interfacing and long-time mental/physical health monitoring.
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高影响因子
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2021
·Vol. 29
·期 2
·1824-1835
·
IF 4.223
Chen Yuan,Chen Yuancai
At present, there are many studies on microbial reduction of Pd (II), although few of these studies assess the bacterial toxicity of Pd (II) before and after reduction by microorganisms. In this study, the toxicity of Pd (II) to Bacillus wiedmannii MSM ( B. wiedmannii MSM) was assessed before and after reduction by live B. wiedmannii MSM cells (referred to as “Pd (0)-loaded cells”) and after biosorption by dead B. wiedmannii MSM cells (referred to as “Pd (II)-loaded cells”). B. wiedmannii MSM is a widely occurring, nonpathogenic aerobic bacterium. Compared with Pd (II), the EC 20 , EC 50 , and EC 80 of Pd (0)-loaded cells increased by 77.73-, 112.75-, and 163.91-fold, respectively, while the EC 20 , EC 50 , and EC 80 of Pd (II)-loaded cells increased by only 11.55-, 9.77-, and 8.29-fold, respectively. The sterilization contribution rates of the mechanisms of action of Pd (0)-loaded cells were ranked in the order of: remaining Pd (II) > oxidative stress > physical puncture. Pd (II) was found to increase cell membrane permeability, glutathione S-transferase (GST) enzyme activity, and reactive oxygen species levels in cells and decrease the cell membrane potential. XPS results indicated that Pd (II) increased the content of polysaccharides and peptides and decreased the content of hydrocarbons in cells. These findings reveal the bactericidal mechanism of toxicity of Pd (II) and Pd (0)-loaded cells on Bacillus wiedmannii MSM and provide an environmentally friendly and inexpensive method for Pd (II) detoxification.
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ACTA PHARMACOLOGICA SINICA
·
2021
·Vol. 43
·期 5
·1264-1273
·
IF 6.15
Ren Zheng-nan,Yang Jun,Zhang Meng-ya,Huang Yi-wen,Song Dong-xiao,Sun Xun,Pan Li-long,Sun Jia
Acute pancreatitis (AP), an inflammatory disorder of the pancreas, is a complicated disease without specific drug therapy. ( R )-4,6-dimethoxy-3-(4-methoxy phenyl)-2,3-dihydro-1H-indanone [( R )-TML104] is a synthesized analog of the natural product resveratrol sesquiterpenes (±) -isopaucifloral F. This study aimed to investigate the effect and underlying mechanism of ( R )-TML104 on AP. The experimental AP model was induced by caerulein hyperstimulation in BALB/c mice. ( R )-TML104 markedly attenuated caerulein-induced AP, as evidenced by decreased pancreatic edema, serum amylase levels, serum lipase levels, and pancreatic myeloperoxidase activity. In addition, ( R )-TML104 significantly inhibited the expression of pancreatic chemokines C–C motif chemokine ligand 2 and macrophage inflammatory protein-2 and the infiltration of neutrophils and macrophages. Mechanistically, ( R )-TML104 activated AMP-activated protein kinase and induced sirtuin 1 (SIRT1) expression. ( R )-TML104 treatment markedly induced the SIRT1-signal transducer and activator of transcription 3 (STAT3) interaction and reduced acetylation of STAT3, thus inhibiting the inflammatory response mediated by the interleukin 6-STAT3 pathway. The effect of ( R )-TML104 on SIRT1-STAT3 interaction was reversed by treatment with a SIRT1 inhibitor selisistat (EX527). Together, our findings indicate that ( R )-TML104 alleviates experimental pancreatitis by reducing the infiltration of inflammatory cells through modulating SIRT1.
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Nanoscale Research Letters
·
2021
·Vol. 16
·期 1
·1-8
·
IF 4.703
Qu Zewen,Li Shi,Feng Wenshuai,Kan Shuting,Gao Xiaohui,Guo Aimin,Li Hongjian,Deng Lianwen,Huang Shengxiang,Zhao Yan,Chen Wei
An accurate sensor to rapidly determine the glucose concentration is of significant importance for the human body health, as diabetes has become a very high incidence around the world. In this work, copper nanoparticles accommodated in porous carbon substrates (Cu NP@PC), synthesized by calcinating the filter papers impregnated with copper ions at high temperature, were designed as the electrode active materials for electrochemical sensing of glucose. During the formation of porous carbon, the copper nanoparticles spontaneously accommodated into the formed voids and constituted the half-covered composites. For the electrochemical glucose oxidation, the prepared Cu NP@PC composites exhibit much superior catalytic activity with the current density of 0.31 mA/cm 2 at the potential of 0.55 V in the presence of 0.2 mM glucose. Based on the high electrochemical oxidation activity, the present Cu NP@PC composites also exhibit a superior glucose sensing performance. The sensitivity is determined to be 84.5 μA /(mmol . L) with a linear range of 0.01 ~ 1.1 mM and a low detection limit (LOD) of 2.1 μmol/L. Compared to that of non-porous carbon supported copper nanoparticles (Cu NP/C), this can be reasonable by the improved mass transfer and strengthened synergistic effect between copper nanoparticles and porous carbon substrates.
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Nano Research
·
2021
·Vol. 15
·期 2
·1338-1346
·
IF 8.897
Li Zhijun,Zhang Mingyang,Zhang Lili,Dong Xiuli,Leng Leipeng,Horton J. Hugh,Wang Jun
Tuning the electronic properties of single atom catalysts (SACs) between the central metal and the neighboring surface atoms has emerged as an efficient strategy to boost catalytic efficiency and metal utilization. Here we describe a simple and efficient approach to create atomically dispersed palladium atoms supported over defect-containing porous ceria nanorod containing palladium up to 0.26 wt.%. The existence of singly dispersed palladium atoms is confirmed by spherical aberration correction electron microscopy and extended X-ray absorption fine structure measurements. This catalyst shows excellent efficiency in hydrodehalogenation reactions at low H 2 pressure under mild conditions, along with satisfactory recyclability and scalability. Density functional theory (DFT) calculations reveal that the high activity stems from the spatial isolation of palladium atoms and the modified electronic structure of palladium confined in defect-containing ceria nanorod. This work may lay the foundation for the facile creation of single atom catalysts within the synthetic community and shed light on the possibility for scale-up production.
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Nano Research
·
2021
·Vol. 14
·期 10
·3439-3445
·
IF 8.897
Li Zixin,Xu Quanlong,Gou Faliang,He Bing,Chen Wei,Zheng Weiwei,Jiang Xu,Chen Kai,Qi Chenze,Ma Dekun
Photoelectrochemical oxygen reduction reaction (ORR) toward H 2 O 2 is highly desirable because only sunlight, O 2 and water are required in the process. However, the corresponding studies are still at its infancy because of the lack of suitable photocathodes, especially inorganic semiconductor photocathodes. In this work, we report CuBi 2 O 4 /CuO (CBO/CuO) heterojunction submicrocrystalline film photocathodes with efficient ORR activity for H 2 O 2 production. The heterojunction film photocathodes were prepared through thermal evaporation of Cu and Bi metals under vacuum and subsequent annealing treatment. Furthermore, the doping of Gd 3+ ions into CBO/CuO could significantly enhance the yield of H 2 O 2 . As a result, the concentration of H 2 O 2 could reach 1.3 mM within 30 min, which is 6 times higher than that obtained on the pristine CBO/CuO photocathode. The theoretical calculations suggested that the introduction of Gd could adjust the electronic structure of CBO surface and promote 2e ORR pathway for selective production of H 2 O 2 . Our work not only provides a new strategy for designing highly efficient photocathode for H 2 O 2 production but also will evoke more interest in photoelectrocatalytic ORR through inorganic semiconductor photocathode.
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Advanced Composites and Hybrid Materials
·
2021
·Vol. 4
·期 3
·459-468
·
IF 5.693
Gu Hongbo,Gao Chong,Zhou Xiaomin,Du Ai,Naik Nithesh,Guo Zhanhu
In this work, in order to ameliorate the hydrophobic property and adsorption capacity of nanocellulose (NC) aerogel, the nanochitosan (NCS) and reduced graphene oxide (rGO) have been introduced into the NC aerogel to construct the NC/NCS/rGO nanocomposite aerogel by a hydrothermal method combined with freeze-drying method. The optimal conditions including the effect of NC and NCS weight percentages for preparation of NC/NCS/rGO nanocomposite aerogel with good porous microstructures and excellent adsorption capacities have been evaluated. The results demonstrate that the NC/NCS/rGO nanocomposite aerogel produced under the fabrication condition of 0.1 wt% NCS and 0.05 wt% NC exhibits a very low density of 9.3 mg cm −3 , a high hydrophobicity with a large water contact angle (115.26°) and the high adsorption capacities of 171.85 ± 3.02, 159.64 ± 1.83, 153.22 ± 2.92, 149.60 ± 6.26, 139.93 ± 3.69, 132.47 ± 3.45, 176.82 ± 4.66, 128.70 ± 0.69, and 120.34 ± 5.57 g g −1 to mineral oil, sesame oil, acetone, ethyl acetate, thiophene, pump oil, waste pump oil, kerosene, and ethyl alcohol, respectively. In addition, this aerogel adsorbent could efficiently and continuously remove/collect the oil from wastewater confirmed by a designed oil/water pump apparatus. As a consequence, this NC/NCS/rGO nanocomposite aerogel has a potential application in the oil and organic solvent adsorption. Graphical abstract
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ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2021
·Vol. 29
·期 7
·10183-10197
·
IF 4.223
Basit Farwa,Liu Jiaxin,An Jianyu,Chen Min,He Can,Zhu Xiaobo,Li Zhan,Hu Jin,Guan Yajing
Brassinosteroids (BRs) are growth-promoting hormones that exhibit high biological activities across various plant species. BRs shield plants against various abiotic stresses. In the present study, the effect of BRs against aluminum (Al) toxicity was investigated through seed priming with 24-epibrassinolide (0.01 μM) in two different rice cultivars. BRs application was found effective in confronting plants from Al toxicity (400 μM). The rice seeds primed with BRs showed enhancement in seed germination energy, germination percentage, root and shoot length, as well as fresh and dry weight under Al-absence and Al-stressed conditions as compared to water-priming. Especially under Al stress, BRs priming promoted the growth of rice seedlings more obviously. Al toxicity significantly increased the Al contents in seedling root and shoot, as well as the MDA concentration, H 2 O 2 production, and the activities of antioxidative enzymes including ascorbate peroxidase, catalase, and peroxidase. Meanwhile, the photosynthetic pigments of seedling reduced under Al stress. When compared to sensitive cultivar (CY-927), these modifications were more obvious in the tolerant variety (YLY-689). Surprisingly, BRs were able to alleviate the Al injury by lowering MDA and H 2 O 2 level and increasing antioxidant activities and photosynthetic pigments under Al stress. The results on antioxidant activities were further validated by gene expression study of SOD-Cu-Zn , SOD-Fe 2 , CATa , CATb , APX02 , and APX08 . It suggested that BRs were responsible for the mitigation of Al stress in rice seedlings by inducing antioxidant activities with an effective response to other seed growth parameters and reduced Al uptake under induced metal stress.
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International Journal of Environmental Science and Technology
·
2021
·Vol. 19
·期 8
·7205-7216
·
IF 2.86
Wu Chunlai,Liu Qiong,Peng Chuanyun,Xu Wenwen
In this work, novel glutamic acid-functionalized SBA-15 was prepared for sensitive and selective lead and copper ions removal from wastewater sample. The material was characterized by X-ray diffractometry, scanning electron microscope, Brunauer–Emmett–Teller surface area measurement and Fourier transform infrared spectroscopy. The as-prepared material maintains the coordination properties of glutamic acid to lead and copper ions and exhibited better adsorption performance than that of pure SBA-15. The effects of different parameters such as pH of the solution, adsorption time, initial concentration, ion selectivity and regeneration were investigated on the Pb(II) and Cu(II) adsorption by the prepared adsorbent. It was found that the adsorption process can reach equilibrium within 20 min and can be described by pseudo-second-order kinetic model. Adsorption isotherm analysis showed that the adsorption was in accordance with Langmuir adsorption isotherm model, and the maximum adsorption capacity of lead and copper ions was 12.14 mg/g and 13.29 mg/g, respectively. In addition, common co-existed ions have no interference on Pb(II) and Cu(II) adsorption. Moreover, adsorption–elution cyclic results demonstrated that Pb(II)- and Cu(II)-adsorbed composite adsorbent could be effectively regenerated by 0.5 mol/L of HCl, and the regenerated adsorbent could be employed for repeated use without significant deterioration in its real performances, demonstrating the good stability of the adsorbents. Therefore, the composite adsorbent can be used potentially to remove Pb(II) and Cu(II) ions from water samples in practical applications for environmental remediation. Graphic abstract Glutamic acid-functionalized SBA-15 can be used as adsorbent for efficient adsorption/desorption of Pb(II) and Cu(II) from wastewater.
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