JOURNAL OF BIOMATERIALS APPLICATIONS
·
2019
·
IF 2.442
Xiaojun Sun,Zhaoyang Li,Zhenduo Cui,Shuilin Wu,Shengli Zhu,Yanqin Liang,Xianjin Yang
In this study, an injectable dopamine-modified alginate hydrogel combined with nano-hydroxyapatite or strontium hydroxyapatite was cross-linked via the in situ slow release of divalent ions from the hydrolysis of D-glucono-δ-lactone (GDL). The cross-linking mechanism involved the chelation of an alginate with divalent ions and the hydrogen-bonding interaction between alginate and catechol groups. As the GDL concentration at 0.5% (w/v) regulated the release of the divalent ions, which enhanced the chelation effect, it was investigated, and inorganic nanoparticles were homogeneously distributed into the hydrogel system to improve the mechanical properties and stabilize the hydrogel network. Furthermore, the effects of dopamine on the improvement of the mechanical properties were investigated. Results demonstrated that the compressive strength of this injectable hydrogel is enhanced by 4.6 times compared with that of the hydrogel without the addition of GDL, and the compressive strength is enhanced by 4.0 times compared with that of the unmodified alginate hydrogel at strain values ranging from 10% to 50%. The hydrogels combined with strontium hydroxyapatite exhibited a more compact, suitable pore size, with a three-dimensional network structure, and the particle size was 50–100 μm; this range of values is considered as optimal for the growth of bone tissues. In addition, the gelation time can be adjusted from a few minutes to a few seconds by adjusting experiment variables. Our study provides a potential method for improving the mechanical properties of bone tissue engineering materials. 1HNMR, FTIR, and UV–vis spectroscopy measurements; XRD and SEM; and pH, ion release, and mechanical tests were carried out.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOCHEMICAL ENGINEERING JOURNAL
·
2023
·
IF 3.9
Xiao-Jian Zhang,Min Cao,Yi-Fei Luo,Meng-Yu Huang,Fang-Ying Zhu,Zhi-Qiang Liu,Yu-Guo Zheng
D-pantolactone (D-PL) is the crucial chiral intermediate for the synthesis of D-pantothenic acid. The asymmetric synthesis of D-PL from ketopantothenic acid lactone (KPL) using conjugated polyketide reductases (CPRs) exhibited high catalytic efficiency and high enantioselectivity. A novel conjugated polyketide reductase KbCPR was isolated from Kazachstania barnettii and the mutant KbCPR Y29K/R65N/K215R was developed with the improved activity by 9.0 folds and the enhanced k cat / K m value by 2.6 folds than the wild-type based on integrated substrate pocket alteration strategy, to alter the substrate channel, to regulate the pocket size, and to facilitate proton transfer. An organic-aqueous biphase biosystem containing 40% dibutyl phthalate (v/v) was constructed to inhibit the hydrolysis of KPL and improve the catalytic efficiency. The time-space yield of 892.9 g L -1 d -1 was achieved, which was higher than ever reported. Furthermore, the organic-aqueous biphase system was employed for the first time in the multi-enzymatic system for D-PL preparation from L-pantolactone (L-PL), without any accumulation of KPL intermediate, high concentration of 1 M L-PL was 95.5% converted to D-PL, with the final production e.e. of >99.9%.
✓ 已验证
产品关联
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2023
·
IF 15.1
Jinxiang Zuo,Donglei Fu,Pengwei Yan,Shuyu Wang,Yabin Li,Linlu Shen,Yizhen Cheng,Jimin Shen,Jing Kang,Zhonglin Chen
In this study, Al-doped NiFe 2 O 4 (NiAl x Fe 2-x O 4 ) was synthesized by the isomorphic substitution strategy, which was further employed for peroxymonosulfate (PMS) activation to remove 2,4-dichlorophenoxyacetic acid (2,4-D) in water. Doping of Al could construct oxygen vacancies (OVs) in the structure of NiAl x Fe 2-x O 4 , which further facilitates the exposure of metal sites for PMS activation. The removal rate of 2,4-D increased greatly compared to the NiFe 2 O 4 -activated process. By the density functional theory (DFT) process, it was verified that Al was more likely to replace Fe rather than Ni. OVs sites have the lowest adsorption energy of H 2 O ( E ads = -0.95 eV), and easily formed surface hydroxyl groups (–OH), which were further replaced by PMS (HSO 5 - ). The formed surface complex Me-O-O-SO 3 - was readily activated via electron transfer between O-O bond, thus promoting the cleavage of it and inducing SO 4 · - generation. This process induced the high performance of the NiAl x Fe 2-x O 4 /PMS system for effective 2,4-D degradation. Furthermore, the purified water showed relatively low toxicity. The present study proposed new insights into the interface mechanism of PMS triggering.
✓ 已验证
产品关联
DOI
高影响因子
DYES AND PIGMENTS
·
2023
·
IF 4.5
Qiushuang Li,Bing Zhao,Xiangdong Kong,Liyan Wang,Wei Kan,Bo Song,Rui Yu,Pengfei Zhang
Designation and application of the luminescent COFs remains a tough challenge and imperative task because of their limited building block structure, the unavailable raw material, the tedious modification process, or the onefold sensing function. Herein, a fluorinated fluorescent covalent organic framework containing a phenanthro[9,10- d ]imidazole moiety [TFPB-PI(F)–COF] had been successfully designed for Cu 2+ and Al 3+ detection relayed by PO 4 3− . TFPB-PI(F)–COF serves as a highly sensing platform with the superior selectivity toward Cu 2+ and Al 3+ based on the coordination interaction and the chelation enhancement quenching (CHEQ) effect, and the detection limits are 2.3 and 4.2 n mol L −1 , respectively. Subsequently, accompanied with the gradual introduction of PO 4 3− , TFPB-PI(F)–COF@M (Cu 2+ and Al 3+ ) complex is wrecked due to electrostatic attraction with the detection limit of 10 n mol L −1 , led to the fluorescent recovery of TFPB-PI(F)–COF. Furthermore, the reversible fluorescent performance by the mode of “on–off–on” is perfect. Besides, the established relayed method can be successfully employed for the analysis of Cu 2+ and Al 3+ in environmental water samples with recoveries of 96.00–104.63 % and RSD <4.62 %. Moreover, the fluorescent images for the visual detection of Cu 2+ is also fabricated in living HeLa cells, which provides a modest contribution and an effective platform to the biological analysis.
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
·
2019
·
IF 3.218
Tongchao Chu,Haoguo Yue,Yue Zhao,Hongmei Du,Yan Zhang,Xiao Han,Jinsheng Zhao,Junhong Zhang
In this study, three novel conjugated electrochromic copolymers including PIPE-1, PIPE-2 and PIPE-3 were successfully synthesized via Stille coupling reaction . In the synthetic process, a novel and multifunctionalized electron functional unit 5,8,14,17-tetrabromoquinoxaline [2′,3′:9,10]phenanthrene[4,5- abc ]phenazine (M2) was selected as the acceptor unit, 6,8-dibromo-3,3-bis-decyl-3,4-dihydro-2 H -thieno[3,4- b ][1,4]dioxepine (M1, brominated ProDOT-decyl 2 ) and bis(trimethylstannane) functionalized indacenodithiophene (M3) as donor units. Cyclic voltammetry (CV), X-ray photoelectron spectroscopy (XPS), spectroelectrochemistry , kinetic switching and thermogravimetry (TG) were performed for the three polymers. The three polymers have band gaps at around 2.0 eV, and the absorption peaks and colors of the polymers can be changed to some extent by changing the feed ratios of the monomers . The three polymers exhibited good thermal stability. More importantly, the three polymers exhibited high optical contrast and fast response time both in the visible and near-infrared regions. The optical contrast of PIPE-1, PIPE-2 and PIPE-3 in the near infrared region were 67.26%, 66.43% and 57.67%, respectively, and the response time of PIPE-2 at 1500 nm was only 0.35 s. PIPE-1 changed from wine red (0 V) to brown (0.90 V) and finally turned blue gray (1.40 V). PIPE-2 changed from rose red color (0 V) to light brown color at 0.90 V, and to light blue color (1.40 V). PIPE-3 changed from magenta (0 V) to light red color at 0.89 V, and to light gray color at 1.02 V, and finally became a blue color (1.40 V), which suggested that the polymer has multi-chromic property.
✓ 已验证
产品关联
DOI
高影响因子
Frontiers in Pharmacology
·
2018
·
IF 3.831
Tsai Hsiang-I,Jiang Lijuan,Zeng Xiaowei,Chen Hongbo,Li Zihuang,Cheng Wei,Zhang Jinxie,Pan Jie,Wan Dong,Gao Li,Xie Zhenhua,Huang Laiqiang,Mei Lin,Liu Gan
The clinical applications of platinum-based antitumor agents are still largely limited by severe side effects as well as multidrug resistance (MDR). To solve these problems, we developed an 1,2-diaminocyclohexane-platinum(II) (DACHPt)-loaded nanoparticle (NP-TPGS-Pt) by self-assembly of poly(amidoamine)-polyglutamic acid-b-D-α-tocopheryl polyethylene glycol 1000 succinate (PAM-PGlu-b-TPGS) and DACHPt. NP-TPGS-Pt showed robust stability and pH-responsive DACHPt release profile in vitro similar to the PEG-containing nanoparticle (NP-PEG-Pt). Meanwhile, in contrast with NP-PEG-Pt, NP-TPGS-Pt exhibited efficient nanoparticle-based cellular uptake by the Pt-resistant A549/DDP human lung cancer cells and caused much more cytotoxicity than free Oxaliplatin and NP-PEG-Pt. Finally, this NP-TPGS-Pt was proved to perform outstanding inhibition of Pt-resistant tumor growth, much superior than free Oxaliplatin and NP-PEG-Pt. Thus, this NP-TPGS-Pt provides a novel powerful nanomedicine platform for combatting multidrug resistant cancer.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2018
·
IF 3.909
Huizhu Wang,Yan Li,Zhihui Ren,Zhongcheng Cong,Mengjie Chen,Lin Shi,Xu Han,Jin Pei
An extraction assay applying microwave-assisted enzymatic treatment for polysaccharides in Rosa roxburghii was developed using response surface methodology. The process parameters were optimized using Plackett-Burman (PB) design and central composite design to enhance the Rosa roxburghii polysaccharide extraction yield. Specific conditions (microwave power, 575 W; microwave time, 18 min; liquid-to-material ratio, 13.5:1 mL/g; and enzyme dose, 6.5 g/mL) generated an experimental yield of 36.21 ± 0.62%, which closely agreed with the predicted value of 35.75%. Purification with a DEAE-52 cellulose column generated two fractions, PR-1 (from 6.2 × 10 3 to 7.4 KDa) and PR-2 (from 559.8 to 106.6 KDa). Subsequently, the antioxidant activity and α- d -glucosidase inhibitory activity of the two polysaccharide fractions were assessed; PR-1 exhibited stronger antioxidant activity and α- d -glucosidase inhibitory activity than PR-2. Finally, the monosaccharide composition of PR-1 was determined by HPLC using a 1-phenyl-3-methyl-5-pyrazolone precolumn derivatization method. The result showed that PR-1 contained mannose , ribose , rhamnose , glucosamine hydrochloride, glucuronic acid , galacturonic acid, glucose, galactose , arabinose and fucose with molar percentages of 2.1%, 0.54%, 2.1%, 0.26%, 1.5%, 22.7%, 24.0%, 26.4%, 19.6% and 0.89%, respectively.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ELECTROCHIMICA ACTA
·
2015
·
IF 4.504
Jiangyi Mao,Xiaoyu Zhang,Shih-Hung Liu,Zhongjin Shen,Xing Li,Wenjun Wu,Pi-Tai Chou,Jianli Hua
The electron-accepting and anchoring group plays a significant role on the optical and electrochemical properties of an organic dye. They also affect the intramolecular charge transfer, the electron injection processes and the adsorption mode, hence the photostability of the dye on TiO 2 films. In this study, we have designed and synthesized two new D-A-π-A dyes ( RD-III and RD-IV ) with 2-(1,1-dicyanomethylene) rhodanine (DCRD) as electron-accepting and anchoring group. For comparison, an analogue of RD-III , namely CA-III , with cyanoacrylic acid (CA) as the acceptor was also prepared. We have carefully examined their optical and electrochemical properties, device performance and electrochemical impedance spectroscopy (EIS). Supplementary support is given by computational approach to gain in-depth insight into the adsorption states and electron contributions. The theoretical calculation of dye/(TiO 2 ) 38 displayed that the angle between the molecule of RD-III and the surface of TiO 2 was only 31.84 0 in contrast to 97.16 0 for CA-III . This adsorption state can facilitate dye aggregation and charge recombination, resulting in a decrease of short circuit current density ( J sc ) and open circuit voltage ( V oc ). Further improvement has been successfully made by adding long alkoxy chains with large steric hindrance. After introducing the alkoxy chains, the dihedral angle between RD-IV and TiO 2 increased to 42.61 0 and the steric hindrance can inhibit dye aggregation and charge recombination. Therefore, higher photoelectric conversion efficiency of 5.53 % was obtained with RD-IV in DSSC devices compared with 4.51 % for DSSC based on RD-III .
✓ 已验证
产品关联
DOI
高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
·
2015
·
IF 4.152
Chune Zhu,Ying Huang,Xiaoying Zhang,Liling Mei,Xin Pan,Ge Li,Chuanbin Wu
The purpose of this study was to compare the properties of exenatide-loaded poly ( d , l -lactic-co-glycolic acid) microparticles (Ex-PLGA-MPs) prepared by a novel ultra-fine particle processing system (UPPS) and spray drying. UPPS is a proprietary technology developed by our group based on the disk rotation principle. Characteristics of the MPs including morphology, particle size distribution, drug content, encapsulation efficiency and in vitro release were comparatively studied. Cytotoxicity of the MPs was examined on A549 cells and the pharmacodynamics was investigated in vivo in type 2 diabetes Sprague-Dawley (SD) rats. Ex-PLGA-MPs prepared by UPPS showed larger particle size, denser surface, greater encapsulation efficiency, less initial burst release, and stable sustained release for more than one month in vitro as compared with the spray drying MPs. Meanwhile, the UPPS MPs effectively controlled the body growth rate and blood glucose in diabetes rats for at least three weeks after a single injection, while the spray drying MPs showed effective control period of about two weeks. UPPS technology was demonstrated to manufacture Ex-PLGA-MPs as a potential sustained release protein/polypeptide delivery system, which is an alternative method for the most commonly used spray drying. This comparative research provides a new guidance for microparticle preparation technology.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Scientific Reports
·
2024
·Vol. 14
·期 1
·1-9
·
IF 3.8
Yuan Fang-Fang,Wang Pei,Han Xiao-Jie,Qin Ting-Ting,Lu Xin,Bai Hai-Jiao
The microreactor with two types of immobilized enzymes, exhibiting excellent orthogonal performance, represents an effective approach to counteract the reduced digestion efficiency resulting from the absence of a single enzyme cleavage site, thereby impacting protein identification. In this study, we developed a hydrophilic dual-enzyme microreactor characterized by rapid mass transfer and superior enzymatic activity. Initially, we selected KIT-6 molecular sieve as the carrier for the dual-IMER due to its three-dimensional network pore structure. Modification involved co-deposition of polyethyleneimine (PEI) and acrylamide (AM) as amine donors, along with dopamine to enhance material hydrophilicity. Remaining amino and double bond functional groups facilitated stepwise immobilization of trypsin and Glu-C. Digestion times for bovine serum albumin (BSA) and bovine hemoglobin (BHb) on the dual-IMER were significantly reduced compared to solution-based digestion (1 min vs. 36 h), resulting in improved sequence coverage (91.30% vs. 82.7% for BSA; 90.24% vs. 89.20% for BHb). Additionally, the dual-IMER demonstrated excellent durability, retaining 96.08% relative activity after 29 reuse cycles. Enhanced protein digestion efficiency can be attributed to several factors: (1) KIT-6’s large specific surface area, enabling higher enzyme loading capacity; (2) Its three-dimensional network pore structure, facilitating faster mass transfer and substance diffusion; (3) Orthogonality of trypsin and Glu-C enzyme cleavage sites; (4) The spatial effect introduced by the chain structure of PEI and glutaraldehyde’s spacing arm, reducing spatial hindrance and enhancing enzyme–substrate interactions; (5) Mild and stable enzyme immobilization. The KIT-6-based dual-IMER offers a promising technical tool for protein digestion, while the PDA/PEI/AM-KIT-6 platform holds potential for immobilizing other proteins or active substances.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Toxics
·
2024
·Vol. 12
·期 1
·83
·
IF 3.9
Kunyu Zhang,Yunyi Yin,Man Lv,Xin Zhang,Meichen Zhang,Jia Cui,Ziqiao Guan,Xiaona Liu,Yang Liu,Yanhui Gao,Yanmei Yang
Limited studies have demonstrated that inorganic arsenic exposure is positively associated with serum vitamin D levels, although the correlation between urinary arsenic species and serum vitamin D has not been investigated in areas of water-borne arsenicosis. A cross-sectional study of 762 participants was conducted in Wenshui Country, Shanxi Province, a water-borne arsenicosis area. The results showed a positive relationship between urinary arsenic species (inorganic arsenic (iAs), methylarsonic acid (MMAV), dimethylarsinic acid (DMAV) and serum 25(OH)D. Log-binomial regression analysis indicated a 0.4% increase in the risk of vitamin D excess for every 1-unit increment in the Box–Cox transformed urinary DMAVafter adjustment for covariates. After stratifying populations by inorganic arsenic methylation metabolic capacity, serum 25(OH)D levels in the populations with iAs% above the median and primary methylation index (PMI) below the median increased by 0.064 ng/mL (95% CI: 0.032 to 0.096) for every one-unit increase in the Box–Cox transformed total arsenic (tAs) levels. Serum 25(OH)D levels increased by 0.592 ng/mL (95% CI: 0.041 to 1.143) for every one-unit rise in the Box–Cox transformed iAs levels in people with skin hyperkeratosis. Overall, our findings support a positive relationship between urinary arsenic species and serum 25(OH)D. It was recommended that those residing in regions with water-borne arsenicosis should take moderate vitamin D supplements to avoid vitamin D poisoning.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Nanoscale
·
2015
·Vol. 8
·期 5
·3100-3118
·
IF 7.394
Di Jiang,Xiaoling Gao,Ting Kang,Xingye Feng,Jianhui Yao,Mengshi Yang,Yixian Jing,Qianqian Zhu,Jingxian Feng,Jun Chen
Drug resistance is the major reason for therapeutic failure during cancer treatment. Chemo-photodynamic combination therapy has potential to improve the treatment efficiency in drug-resistant cancers, but is limited by the incompatible physical properties of the photosensitizer with a chemo-drug and poor accumulation of both drugs into the inner areas of the tumor. Herein, a novel drug delivery system was designed by incorporating the photosensitizer, chlorine 6, chemically in the shell and the chemo-drug, doxorubicin, physically in the core of D-α-tocopheryl polyethylene glycol 1000 succinate-poly(lactic acid) (TPGS-PLA) nanoparticles with a targeting ligand, tLyp-1 peptide, decorated over the surface (tLyp-1-NP). This nanoparticle with a high drug loading capacity of both the photosensitizer and chemo-drug is expected to realize chemo-photodynamic combination therapy of drug-resistant cancer and simultaneously achieve the specific deep penetration and accumulation of drugs into the inner areas of tumor. tLyp-1-NP was prepared via a nanoprecipitation method and it exhibited a uniformly spherical morphology with a size of approximately 130 nm. After appropriate irradiation, tLyp-1-NP showed high cellular uptake and strong cytotoxicity in both human umbilical vein endothelial cells (HUVEC cells) and doxorubicin-resistant human breast adenocarcinoma cells (MCF-7/ADR cells) in vitro. After intravenous administration, compared with the unmodified NPs, tLyp-1-NP was found to have superior tumor targeting ability and more potent reversion of doxorubicin-resistant cancer. The terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling and the hematoxylin and eosin staining of the treated tumors further demonstrated the anti-tumor efficacy of tLyp-1-NP in the presence of a laser. These observations collectively suggest the potential of tLyp-1-NP for the actively targeting chemo-photodynamic combination therapy of drug-resistant cancer.
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）