JOURNAL OF BIOTECHNOLOGY
·
2015
·
IF 2.871
Yu-Cai He,Zhi-Cheng Tao,Yun Ding,Dan-Ping Zhang,Yin-Qin Wu,Yun Lu,Feng Liu,Yu-Feng Xue,Cheng Wang,Jian-He Xu
To avoid adding NAD + and effectively transform ethyl 4-chloro-3-oxobutanoate, the mixture of l -glutamine (200 mM) and d -xylose (250 mM) was added into in n -butyl acetate–water (10:90, v/v) biphasic system instead of NAD + for increasing the biocatalytic efficiency. To further improve the synthesis of optically pure ethyl ( R )-4-chloro-3-hydroxybutanoate (>99% ee ), β-cyclodextrin was also added into this reaction media, and ethyl ( R )-4-chloro-3-hydroxybutanoate (>99% ee ) could be effectively synthesized from 800 mM ethyl 4-chloro-3-oxobutanoate in the yield of 100% by whole-cells of recombinant E. coli CCZU-A13. Finally, the possible mechanism for improving the reductase activity by supplementation of l -glutamine, d -xylose and β-CD was proposed. In conclusion, this strategy has high potential for the effective biosynthesis of ethyl ( R )-4-chloro-3-hydroxybutanoate (>99% ee ).
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高影响因子
ANALYST
·
2012
·Vol. 137
·期 18
·4235-4240
·
IF 4.23
Xiaoyu Mu,Li Qi,Ying Shen,Haizhi Zhang,Juan Qiao,Huimin Ma
A novel amino acid ionic liquid (AAIL) with L-ornithine (L-Orn) as anion was successfully synthesized, and subsequently applied as an available chiral ligand coordinated with Zn(II) in a chiral ligand exchange capillary electrophoresis (CLE-CE) system for the enantioseparation of dansyl amino acids (Dns-D,L-AAs). The influence of key parameters, such as buffer pH, concentration ratio of Zn(II) to ligand and complex concentration, was investigated in detail. Eleven pairs of Dns-D,L-AAs enantiomers were baseline separated and three pairs were partly separated under the optimum conditions. For exploring its potential application, the quantitative features of this proposed method were studied. Good linearity (r2 = 0.999) and favorable repeatability (RSD ≤ 3.4%) were obtained by using Dns-D,L-Met as the test analyte. Finally, this method was employed to investigate the inhibition efficiency of D-amino acid oxidase (DAAO) inhibitors, which may pave a new way for the high-throughput screening of enzyme inhibitors and relevant drug discovery.
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高影响因子
JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
·
2018
·
IF 0.827
Liwei Cao,Tian Wei,Yihui Shi,Xiaofang Tan,Jianxin Meng
Two rapid and sensitive analytical methods are developed for the determination of D-penicillamine (D-PEN) and tiopronin (TP) through high-performance liquid chromatography with fluorescence detection (HPLC-FLD) and capillary electrophoresis with laser-induced fluorescence detection (CE-LIF). A boron-dipyrrolemethene (BODIPY) fluorescence labeling reagent, 1,3,5,7-tetramethyl-8-bromomethyl-difluoroboradiaza-s-indacene (TMMB-Br) was successfully applied to derivatize these two thiol drugs. Fluorescein was used as the internal standard (IS) for the quantification of D-PEN and TP in CE-LIF. The derivatization and separation conditions were optimized carefully. Under the optimum conditions, the HPLC and CE separation of D-PEN and TP could be achieved within 12 min. The limits of detection were as low as 2.0 nmol/L for HPLC-FLD and 0.47 nmol/L for CE-LIF. The drugs in human urine and serum samples were determined successfully, and the recoveries were 95.0–06.7% and 95.2–104.3%, respectively.
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高影响因子
Nanoscale
·
2015
·Vol. 8
·期 5
·3100-3118
·
IF 7.394
Di Jiang,Xiaoling Gao,Ting Kang,Xingye Feng,Jianhui Yao,Mengshi Yang,Yixian Jing,Qianqian Zhu,Jingxian Feng,Jun Chen
Drug resistance is the major reason for therapeutic failure during cancer treatment. Chemo-photodynamic combination therapy has potential to improve the treatment efficiency in drug-resistant cancers, but is limited by the incompatible physical properties of the photosensitizer with a chemo-drug and poor accumulation of both drugs into the inner areas of the tumor. Herein, a novel drug delivery system was designed by incorporating the photosensitizer, chlorine 6, chemically in the shell and the chemo-drug, doxorubicin, physically in the core of D-α-tocopheryl polyethylene glycol 1000 succinate-poly(lactic acid) (TPGS-PLA) nanoparticles with a targeting ligand, tLyp-1 peptide, decorated over the surface (tLyp-1-NP). This nanoparticle with a high drug loading capacity of both the photosensitizer and chemo-drug is expected to realize chemo-photodynamic combination therapy of drug-resistant cancer and simultaneously achieve the specific deep penetration and accumulation of drugs into the inner areas of tumor. tLyp-1-NP was prepared via a nanoprecipitation method and it exhibited a uniformly spherical morphology with a size of approximately 130 nm. After appropriate irradiation, tLyp-1-NP showed high cellular uptake and strong cytotoxicity in both human umbilical vein endothelial cells (HUVEC cells) and doxorubicin-resistant human breast adenocarcinoma cells (MCF-7/ADR cells) in vitro. After intravenous administration, compared with the unmodified NPs, tLyp-1-NP was found to have superior tumor targeting ability and more potent reversion of doxorubicin-resistant cancer. The terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling and the hematoxylin and eosin staining of the treated tumors further demonstrated the anti-tumor efficacy of tLyp-1-NP in the presence of a laser. These observations collectively suggest the potential of tLyp-1-NP for the actively targeting chemo-photodynamic combination therapy of drug-resistant cancer.
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PubMed
高影响因子
TETRAHEDRON-ASYMMETRY
·
2014
·
IF 2.165
Rui-Jie Chen,Gao-Wei Zheng,Yan Ni,Bu-Bing Zeng,Jian-He Xu
A new NADH-dependent carbonyl reductase Rh CR capable of efficiently reducing the ε-ketoester ethyl 8-chloro-6-oxooctanoate (ECOO) to give ethyl ( S )-8-chloro-6-hydroxyoctanoate [( S )-ECHO], an important chiral precursor for the synthesis of ( R )-α-lipoic acid, was identified from Rhodococcus sp. ECU1014. Using recombinant Escherichia coli cells expressing Rh CR and glucose dehydrogenase used for the regeneration of cofactor, 440 g L −1 (2 M) of ECOO were stoichiometrically converted to ( S )-ECHO in a space–time yield of 1580 g L −1 d −1 without the external addition of any expensive cofactor.
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高影响因子
TALANTA
·
2017
·
IF 4.162
Quanqing Zhang,Yuanyu Huang,Haofan Sun,Yangyang Gan,Huali Shen,Bin Jia,Pengyuan Yang
Novel 3-D floor-like ordered mesoporous carbon functionalized graphene composites, FLOMC-GO, with high graphitized carbon contents were successfully synthesized using a soft template method. The one-pot sol-gel method was employed to prepare the silica soft template. Then, the sandwich-like composites were further combined together to form a 3-D structure through pre-carbonization and carbonization . During these procedures, the sulphonyl and sulfide bridges were formed by cross-linking processes to connect the phenyl rings. The prepared FLOMC-GO was confirmed to have a large pore volume (1.03 cm 3 g −1 ), high BET surface area (544.99 m 2 g −1 ) and well-ordered mesoporous structure with a narrow pore-size concentrated at 3.74 nm. The content of carbon reached 80% and was highly graphitized. By taking advantage of the interactions between carbon and glycans, FLOMC-GO was utilized to enrich N-linked glycans from OVA and human serum. As expected, excellent size-exclusion was found during the enrichment of N-glycans released from OVA, and 25 N-linked glycans were identified. The intensity of glycans enriched by FLOMC-GO was 7 times to the result of active carbon, while the ratio of OVA digestion to BSA interfering proteins increased to 1:50. Additionally, 31 N-linked glycans in total were enriched from human serum. The relatively easy synthesis as well as ability to enrich N-linked glycans with high selectivity and efficiency makes FLOMC-GO a promising adsorbent material for the discovery of human serum biomarkers for disease diagnosis.
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高影响因子
Journal of Macromolecular Science Part B-Physics
·
2020
·
IF 0.911
Dan Wang,Jianjun Fang,Wenjuan Liu,Tianlong Huang,Yanmin Zhao,Jianglong Duan,Hailin Xie,Ping Chen,Ziwei Zhou,Zhihua Zhou
Poly(L-lactide-co-glycolide-co-ε-caprolactone) (PLLGC) and 1,4-butanediamine modified maleated poly(D,L-lactide-co-glycolide) (BMPLGA) were both synthesized by ring-opening polymerization with stannous octoate as catalyst. Then PLLGC/BMPLGA blend porous films were prepared by using NaCl particles as a porogen through a solvent casting/particle leaching method. The PLLGC/BMPLGA blend porous films were characterized by Fourier transform infrared spectroscopy, gel permeation chromatography and scanning electron microscopy. The tensile properties of the PLLGC/BMPLGA porous films were also studied. The results showed that the content and size of the NaCl porogens had significant effects on the porosity of the PLLGC/BMPLGA porous films. The tensile strength and elongation percentage of the porous films decreased with increasing porogen content. Thus, both the pore structure and mechanical properties of the porous PLLGC/BMPLGA blend films can be controlled by changing the amount and size of the porogen.
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高影响因子
JOURNAL OF NANOBIOTECHNOLOGY
·
2026
·
IF 15
Xia Liu,Hui Li,Ningning Shan,Bingxin Guan,Yang Jiang,Chengyun Zheng,Leisheng Zhang,Dexiao Kong
Aplastic anemia (AA) is a debilitating disorder marked by bone marrow failure, frequently associated with dysregulated T cell activity. The present study explored the therapeutic potential of anti-CD3 antibody-modified calcium silicate nanoparticles loaded with novel 7H-pyrrolo[2,3-d]pyrimidine derivatives (antiCD3-pCaSiNP@NPDP) for AA treatment. Whole-transcriptome sequencing and bioinformatics analysis identified interleukin-2-inducible T-cell kinase (ITK) as a critical regulator of T cell function in AA. In vitro experiments demonstrated that ITK enhances T cell proliferation and promotes differentiation toward inflammatory subsets, thereby contributing to disease progression. The newly developed NPDP derivatives effectively inhibited ITK activity. Targeted delivery of NPDP via antiCD3-pCaSiNP nanoparticles selectively suppressed ITK expression in T cells, resulting in reduced inflammatory T cell proliferation and increased regulatory T cell populations. In an AA mouse model, administration of antiCD3-pCaSiNP@NPDP nanoparticles markedly improved hematopoietic recovery and immune balance. The findings indicate that nanoparticle-mediated ITK inhibition represents a promising therapeutic strategy for restoring immune and bone marrow function in AA.
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PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2026
·
IF 12.5
Yan Liang,Li Xue,Feifei Xiang,Xiaojian Zhao,Lingmin Liang,Fengyi Liang,Yuhao Zhan,Ziteng Guo,Fangjun Liu,Yong Yang
A multi-interfacial NiO@In 2 O 3 /ZnO porous heterojunction array was designed. • The heterojunction array enabled highly efficient room temperature ammonia detection. • Multi-phase interfaces greatly enhanced the ammonia adsorption and charge transfer. • Porous array design and light activation further promoted the sensing reaction. • The work clarified the important role of multi-phase interfaces in gas sensing. Heterojunction engineering provides an effective means to enhance the gas sensing performance of metal oxide semiconductors through band structure optimization and interface effects. However, current research on heterojunction engineering primarily centers on binary heterojunctions (including p-n, n-n, and p-p junctions), while studies concerning ternary or multicomponent metal oxide heterojunctions for gas sensing remain exceedingly limited and challenging. Herein, a ternary NiO@In 2 O 3 /ZnO porous heterojunction array is fabricated in-situ on commercial alumina sensing substrate via a facile liquid-phase chemical reaction combined with topotactic transformation. The heterojunction array enables highly efficient room temperature ammonia detection under visible light illumination, exhibiting a high response of 263% towards 100 ppm ammonia, a fast recovery of 21 s, and a low detection limit of 0.483 ppm. Moreover, it displays excellent selectivity, strong resistance to humidity interference, and stable long-term performance. First-principles calculations integrated with spectroscopic characterization suggest that the construction of ternary NiO@In 2 O 3 /ZnO heterojunction with multi-phase interfaces greatly enhances the ammonia surface adsorption and promotes the interfacial charge transfer efficiency. Interestingly, there exists a notable positive correlation between the d-band center position of sensing materials and their adsorption behavior as well as sensing performance. This work not only achieves a multi-dimensional enhancement in the room temperature ammonia sensing performance of metal oxide semiconductors through ternary heterojunction construction, porous array design and external light field activation, but also significantly enhances the gas sensing mechanism of ternary heterojunctions at atomic and electronic scale, offering new insights for the rational designing of high-performance resistive gas sensors via multi-phase interfaces.
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高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2026
·
IF 12.5
Ziyi Shao,Kun Fan,Hua Ying,Yiting Chen,Jiayu Yan,Jiajun Zhang,Jiahao Zhang,Zhirui Niu,Yanzhong Zhen,Jingyu Ren
Crystal field splitting energy governed the doping-induced d-band shift. • Anti-bonding orbital activation mechanism for O 3 , H 2 O 2 and PS was proposed. • Molecular dipole moment was the dominant factor for pollutant degradation. Modulating the d-band center of transition metals by doping engineering is an efficient strategy to enhance their catalytic efficiency. However, there remains a lack of systematic theoretical explanation for how doping engineering modulates the d-band center and activates antibonding orbitals at the electronic structure scale. Herein, using α-Fe 2 O 3 (αFO) as a model transition metal, machine learning, density functional theory calculations and experimental characterization were integrated to elucidate the doping induced d-band center shifts. The results reveal that dopants modulated the crystal field splitting energy of transition metals, which in turn tailors the d-band center, thereby enhancing the catalyst's ability to activate the antibonding orbitals of reactive species. This mechanism rationalized the efficient degradation of metronidazole observed in the Cu-αFO/DBD/persulfate process. Furthermore, to elucidate the degradation mechanism at the molecular level, this study investigated the structure-activity relationship, specifically how the molecular structure of the pollutants and the catalytic system influence the degradation efficiency. The data revealed the correlation between molecular properties (e.g., dipole moment and energy of the lowest unoccupied molecular orbital) and degradation kinetics. The proposed d-band center modulation and antibonding orbital activation mechanism provides both a theoretical foundation and a practical pathway for the rational design of high performance transition metal catalysts. Download: Download high-res image (388KB) Download: Download full-size image
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高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2023
·
IF 13.6
Jinrui Dong,Pin Li,Xiaoyuan Ji,Yong Kang,Xue Yuan,Jingchun Tang,Boxiong Shen,Huajiang Dong,Honghong Lyu
Photocatalytic oxidation technology holds promise for ideal advanced treatment of antibiotic wastewater. Single-atom catalysts (SACs) are a new hotspot in catalytic science, but the photochemical studies on the removal of antibiotics from water and biocompatibility after entering the environment are scarce. In this work, we prepared a single Mn atom immobilized on N-doped biochar (Mn@N-Biochar) by impregnation calcination method for enhancing photocatalytic degradation of sulfanilamide (SNM) in different types of various water systems. Compared with the original biochar, Mn@N-Biochar showed enhanced SNM degradation and TOC removal capacity. DFT calculation concluded that the electrons of d‐orbital (Mn) and p-orbital (N) altered the electronic structure of biochar and enhanced the photoelectric performance. It was shown that Mn@N-Biochar caused negligible systemic inflammation and tissue damage when given orally in mice, and also did not alter cell death and ROS production in human lung, kidney, and liver cells, as compared with biochar. We are convinced that Mn@N-Biochar could enhance the photocatalytic degradation of antibiotics while maintaining biocompatibility, which could be a promising strategy for wastewater treatment .
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产品关联
DOI
PubMed
高影响因子
Frontiers in Bioengineering and Biotechnology
·
2023
·
IF 5.7
Sun Xiaolong,Hu Jiahuan,Wang Yifeng,Luo Xi,Huang He,Fu Yongqian
The main challenges in bio-catalysis of d-phenyllactic acid (D-PLA) are poor tolerance of lactate dehydrogenase (LDH) to harsh environmental conditions and inability to recycle the catalyst. A novel magnetic framework composite was prepared as solid support for the immobilization of enzymes via one-pot encapsulation in this study. LDH/MNPs@MAF-7 was synthesized by the one-pot encapsulation of both LDH and magnetic nanoparticles (MNPs) in MAF-7. The LDH/MNPs@MAF-7 showed stable biological activity for the efficient biosynthesis of D-PLA. The structure and morphology of LDH/MNPs@MAF-7 were systematically characterized by SEM, FT-IR, XRD, VSM, XPS, TGA and N2 sorption. These indicated that LDH/MNPs@MAF-7 was successfully synthesized, exhibiting enhanced resistance to acid and alkali, temperature and organic solvents. Furthermore, the bio-catalyst could be separated easily using a magnet, and the reusability was once considerably expanded with 80% of enzyme activity last after eight rounds of recycling. Therefore, LDH/MNPs@MAF-7 could be used as a potential biocatalyst for the biosynthesis of D-PLA due to its good stability and recovery properties.
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DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）