NEW JOURNAL OF CHEMISTRY
·
2024
·
IF 2.7
Jiamin Wang,Hao Lin,Mengli Liu,Bo Xiong,Dingyuan He,Jiasheng Tu,Dongsheng He
Cationic polymers are a class of promising carriers for nucleic acid delivery. However, the balance between biosafety and transfection efficiency is still a major obstacle for nucleic acid delivery. Accurate structural design can effectively evaluate the relationship between structural factors and nucleic acid delivery. Previous series of vectors optimized based on artificial amino acid Stp have been shown to effectively improve gene transfection. Here, a series of sequenced-defined oligomers containing hydrophobic oleic acid with different topologies, different charge densities and with or without crosslinking cystine domains were designed and synthesized for a better understanding of the structure activity relationship. The oligomers were further mixed with either pDNA or siRNA to form polyplexes. The particle size, zeta potential, polyplexes formation properties, in vitro stability and reductive responsiveness of the polyplexes were investigated. In addition, studies regarding the transfection efficiency, cellular uptake, cellular trafficking, cytotoxicity and hemolysis were performed. The resulting oligomers display general good complexation ability with pDNA and siRNA with enhanced stability and redox sensitivity. some of the oligomers showed adequate or surpassed transfection efficiency compared with PEI. The polyplexes formed with oligomer #1 have good cellular uptake and endo-lysosome escape property. In addition, all the oligomers and the polyplexes showed low cytotoxicity and hemolysis, demonstrated high biocompatibility. In summary, the results evidenced that proper charge density with hydrophobic oleic acid and thiol crosslinking cystine improves the overall performance of delivering pDNA and siRNA.
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JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
·
2024
·
IF 4.5
Wenyuan Liu,Bo Hou,Dewei Ma,Mingwei Peng,Hongli Mao,Wei Liu,Zhongwei Gu,Siyuan Chen
Transdermal delivery is an attractive route for the treatment of skin disease. However, its therapeutic effectiveness is significantly hampered by the stratum corneum and cell membrane barriers. Herein, we reported a novel liposomes incorporated microneedles (MNs) delivery platform to address these two key challenges. Conventional liposomes were surface modified with membrane-anchoring fusogenic proteins mimicking pH-responsive, comb-like pseudopeptides containing relatively long alkyl side chains. The liposomes-containing MNs were fabricated with biodegradable and biocompatible silk fibroin with sufficient mechanical strength using micromolding technology. The degradation and release profile of liposomes from the MNs delivery platform can be manipulated by controlling the liposomes to silk fibroin ratio, and the released liposomes well-maintained their original spherical shape and pH-responsive functionality. Remarkably enhanced in vitro skin permeation was observed in the polymer modified liposomes incorporating MNs delivery platform compared to the ones without MNs facilitation. Enhanced cellular uptake and potency against cancer cells of the pH-responsive, comb-like pseudopeptides modified liposomes incorporating MNs delivery platform were demonstrated. These results suggest its potential applications for efficient skin penetration and enhanced cellular uptake, which is of crucial importance for skin disease treatment.
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JOURNAL OF MOLECULAR STRUCTURE
·
2024
·
IF 4
Zi-Ping Wang,Yu Wang,Xin-Yu Li,Lin-Fang Jia,Ai-Zhen Yang,Wen-Ting Zhao,Yi Jia,Bao-Yi Yu,Han-Qing Zhao
The synthesis and characterization of three 2D to 3D metal-organic frameworks (MOFs) with mixed ligands, namely {[Cd(DINT)(TPA)(H 2 O)]ACN} n (BAO24) (H 2 TPA = tere phthalic acid, DINT = 1,4-di(imidazol-1-yl)naphthalene, and ACN = acetonitrile), {[Cd(DINT)(IPA)]DMF} n (BAO25) (H 2 IPA = iso phthalic acid and DMF = N,N -dimethylformamide), and [Cd(DINT)(PA)] n (BAO26) (H 2 PA = phthalic acid), were reported herein. The structures and purities of the MOFs were revealed and confirmed by single-crystal X-Ray diffraction, Fourier transform infrared spectroscopy, powder X-Ray diffraction, thermogravimetric analysis, and elemental analysis. The MOFs exhibited a fluorescence emission peak around λ em = 340 nm with relative excitation wavelengths at both 230 and 290 nm. Since the MOFs showed similar fluorescence properties, BAO24 was selected as a representative MOF in the fluorescent sensing processes. The result showed that BAO24 exhibited a high sensitivity for vitamin B2. However, when sensing pesticides, the sensor performed differently at each excitation wavelength. At λ ex = 230 nm, the sensor was quenched the most significantly with the nitenpyram analyte, while at λ ex = 290 nm, the highest fluorescence quenching was achieved by the imidacloprid analyte.
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FOOD CHEMISTRY
·
2024
·
IF 8.5
Kai Wang,Hui Li,Wenya Liu,Pan Liu,Chuang Jiang,Wanqi Jiang,Yaqing Xiao,Yingnan Liu
Based on the previously developed Al 3+ /CuNCs probe, the matrix effect (ME) derived from protein was investigated in the fluorescence determination of fluoroquinolones (FQs) residues in eggs, and a sample pretreatment method was established to rapidly reduce the ME. Molecular docking results indicated that the three main egg proteins and FQs were bound by hydrogen bond, van der Waals force, and alkyl groups, leading to the loss of FQs response signal, and then produced ME. After trichloroacetic acid treatment, the mean particle size of egg matrix decreased, the protein secondary structure changed, and the crystal structure was destroyed. The corresponding ME of the supernatant obtained by centrifugation was only 0.7 %. The standard addition experiment confirmed that the proposed pretreatment method improved the accuracy and reliability of the fluorescence probe. This work is helpful to promote the practical application of fluorescence analysis in the rapid monitoring of food safety hazard factors.
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高影响因子
FOOD RESEARCH INTERNATIONAL
·
2024
·
IF 7
Zihuan Wu,Haiqin Chen,Bo Yang,Jianxin Zhao,Wei Chen
The study aimed to determine the chemical structures of octadecatrienoic acid isomers produced by probiotics through the bioconversion of α-linolenic acid and to assess their antioxidant capacities. The chemical structures were identified using nuclear magnetic resonance spectroscopy (NMR) and mass spectrometry (MS), while the antioxidant capacities were evaluated in vitro and in cellular. The NMR signals obtained allowed for definitive characterization, with the main ion fragments detected being m / z 58.0062, 59.0140, 71.0141, 113.0616, 127.0777, and 181.5833. Compounds at concentrations below 40 μM maintained the antioxidant capacity of HepG2 cells by protecting endogenous antioxidative enzymes and mitochondrial membrane potential. However, doses higher than 40 μM increase oxidative damage and mitochondrial dysfunction. These results confirmed the structure of the probiotic-derived compound as trans9, trans11, cis15-conjugated linolenic acid. Additionally, appropriate doses of CLNA can alleviate oxidative stress induced by AAPH, while high doses aggravate cellular damage. These findings provide foundational information for the further exploration of probiotic-derived edible lipids.
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高影响因子
Materials Today Bio
·
2025
·
IF 10.2
Xinming Wang,Yiming Zhong,Bei Qian,Shixing Huang,Qiang Long,Haonan Zhang,Qiang Zhao,Xiaofeng Ye
Cardiovascular disease remains a leading cause of mortality, highlighting the critical need for novel therapeutic strategies. RNA-based therapeutics, including siRNA and mRNA, offer promising approaches for cardiac diseases, yet their clinical application is limited by low heart specificity and suboptimal delivery methods. Lipid nanoparticles (LNPs) are widely used for RNA delivery but often accumulate in non-cardiac tissues, reducing their effectiveness. To address this, an extracellular matrix (ECM)-LNP composite is developed for targeted RNA delivery to the myocardium. The LNPs are conjugated to the ECM scaffold to enhance RNA retention. In vivo experiments demonstrate effective mRNA delivery and expression within the heart, with preferential targeting towards immune cells. Epidermal growth factor receptor (EGFR), a key regulator of cell proliferation and inflammation, is found to affect macrophage polarization in this study. The therapeutic potential of EGFR siRNA delivered via ECM-LNP composite is further explored in a mouse model of myocardial infarction (MI). Results indicate that ECM-siEGFR@LNP reduces cardiac fibrosis and promotes M2 macrophage polarization. This effect is associated with down-regulation of the EGFR-AKT signaling pathway. In conclusion, this study presents an injectable platform for heart-specific RNA delivery and sheds light on the role of EGFR signaling in the cardiac repair process.
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ANALYTICAL CHEMISTRY
·
2025
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IF 6.7
Yaqin Wang,Huiping Yang,Zhaoquan Li,Qian Mo,Shuang Li,Longda Li,Fan Yang,Xinchun Li,Yong Huang
Mimicking ion-gating function with artificial nanopores empowered by well-designed responsive DNA architectures represents one of the leading maneuvers in the nanopore sensing field. However, due to the rarely preponderant orientation and uncontrollable assembly of conventional DNA constructs at heterogeneous interfaces, the recognition ability and gating efficiency remain a considerable challenge. Here, we show that manipulating the ion-gating efficiency can be readily achieved by the assembly of responsive tetrahedral DNA nanostructures in glass nanopipettes. We design a set of 3D-DNA nanostructures consisting of size-tunable DNA tetrahedrons and Pb2+-dependent DNAzyme structural domain in the nanopipettes, which served as the ion gate and recognition element, respectively, and thus enabled the sensing of Pb2+ in a label-free manner. Using the constructed 3D-DNA-nanostructured ion-gating systems, we can program stimulus–response capacity and ionic current rectification behaviors in the Pb2+ sensing events. We found that larger-size DNA tetrahedrons resulted in more efficient ion gating and importantly could achieve dynamic linear response to Pb2+, with the concentration spanning from 10 pM to 10 μM. Finite element simulations revealed that steric hindrance, rather than surface charge, may be a principal cause for regulating trans-pore ion transport. This work opens an avenue for the design of DNA nanostructure-based responsive nanopores for sensing applications.
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高影响因子
CELL BIOCHEMISTRY AND FUNCTION
·
2025
·Vol. 43
·期 7
·e70100
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IF 2.7
Jie Xiao,Weiwei He,Xiaoyang Fang,Rongrong Zhou,Ao Huang,Yikun Wang,Qing Du,Linben Xu,Fei Cheng,Hongliang Zeng
Smilax glabra Roxb. is a natural herb, exhibits significant uric acid lowering effect in clinical. However, the specific mechanism remains to be clarified. To study this problem, intraperitoneal injection of Potassium oxazinate (PO) and Hypoxanthine (HX) induced hyperuricemia in mice model, and mice were divided into control, model, and model plus ethanol extract (TFL), ethyl acetate extract (TFL_Y), n-butanol extract (TFL_Z), and residual aqueous extract (TFL_S) of Smilax glabra Roxb., And then a follow-up test. Furthermore, possible pharmacological components were detected by UPLC-Q-TOF-MS. The results showed that serum creatinine, blood urea nitrogen, d -lactate, lipopolysaccharide levels and xanthine oxidase. They could upregulate renal OAT1 and ABCG2, downregulate renal URAT1, reduce renal tubular dilation and interstitial inflammatory cell infiltration. Moreover, they could upregulate intestinal tight junction proteins, increase the number of intestinal goblet cells, and repair damage to the intestinal barrier. Moreover, 16S rRNA sequencing analysis showed that restored intestinal microbiota by increasing probiotic bacteria ( Candidatus Saccharimonas , Lachnospiraceae NK4A136_group ) and reducing pathogenic bacteria ( Helicobacter ). Furthermore, it was found that all control taint flavonoids such as Neoastalbin, Astibin, Neoiosastilbin, Isoastalbin, Engeletin, and Isoengeletin. Summary This study elucidates the multi-target mechanisms underlying the urate-lowering effects of Smilax glabra Roxb., including modulation of renal urate transporters (OAT1/ABCG2/URAT1), improvement of intestinal barrier function, and restoration of gut microbiota homeostasis. Flavonoid (e.g., neoastilbin and isoastilbin) were identified as the main active ingredients through UHPLC-Q-TOF-MS analysis. For the first time, we comprehensively revealed the “gut-kidney axis” mechanism of Smilax glabra Roxb., identified critical targets in renal transporters and gut microbiota, discovered bioactive flavonoid markers for quality control, and provided theoretical support for its clinical application and drug development.
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CANCER CELL
·
2025
·
IF 44.5
Yang Yang,Chuanyan Yang,Xuezhu Chen,Yibin Jiang,Xuejiao Lei,Kang Ma,Yulian Quan,Tianran Li,Chenfu Guo,Yijing Meng,Lin Kang,Xinyu Zhang,Long Jin,Jiafeng Huang,Ning Mu,Zexuan Yan,Qinghua Ma,Shuai Wang,Yanxia Wang,Yong-Ning Shang,Cong Chen,Yu Shi,Shukun Hu,Likun Yang,Chuan Lan,Rong Hu,Ying Zhang,Xia Li,Yunqing Li,Chong Liu,Yu-Hai Wang,Fei Li,Hua Feng,Xiu-Wu Bian,Tunan Chen
Glioblastoma (GBM), the most aggressive primary brain tumor, is shaped by its integration into neural networks. While glutamatergic input is linked to tumor progression, the broader architecture and function of neuron-glioma connectomes remain unclear. Using monosynaptic rabies tracing, we map brain-wide neural input to patient-derived xenografts and reveal a consistent organizational logic: local inputs are primarily glutamatergic, while long-range connections exhibit diverse neurotransmitter profiles, with basal forebrain cholinergic projections emerging as a conserved input across sites. Functionally, presynaptic acetylcholine release promotes GBM progression through muscarinic receptor CHRM3 in a circuit-specific manner. Mechanistically, glutamatergic and cholinergic signals converge to enhance glioma calcium transients but diverge in temporal transcriptional control, with their dual blockade producing additive anti-tumor effects. Therapeutically, the anticholinergic drug scopolamine attenuates glioma growth, whereas the acetylcholinesterase inhibitor donepezil exacerbates disease. These findings reveal the complexity of neuron-glioma connectivity, highlighting long-range neuromodulatory pathways as promising therapeutic targets in GBM.
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高影响因子
aBIOTECH
·
2025
·
IF 5
Zhang Wenyang,Wang Yanjiao,Li Wenyan,Wu Shaowen,Chen Yuanyuan,Ye Mingyang,Huang Wenjie,Fernie Alisdair R.,Yan Shijuan
Protein post-translational modifications such as phosphorylation and S -nitrosylation regulate protein functions and cellular programs in eukaryotes. Moreover, extensive evidence suggests crosstalk between these modifications. However, we lack a comprehensive method for the simultaneous detection and analysis of multiple post-translational modifications. Here, we present an optimized workflow that identifies phosphorylation and S -nitrosylation sites using a novel phosphate affinity tag switch technique. Validation with model proteins and complex biological samples confirmed the high sensitivity, coverage, and reproducibility of this method. Applying this method to Arabidopsis thaliana seedlings revealed 12,552 phosphorylation sites and 6,108 S -nitrosylation sites, representing the largest single-study dataset of S -nitrosylation sites to date. This approach enhances our understanding of post-translational modification dynamics in plant signaling, stress responses, and metabolism.
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MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Siyu Wu,Xiaojuan Niu,Jiali Chen,Wangni Meng,Han Tao,Jianli Zhou,Yuangen Wu
Kanamycin (KAN) can generate a serious threat to human health due to its tendency to accumulate in food. Current colorimetric strategy for KAN detection still faces some challenges, and nanozyme-based colorimetry is an ideal alternative approach. Herein, a non‑copper-based multivalent laccase mimic named Mn-Car is first synthesized through a self-assembly of manganese ions and the endogenous biomolecule carnosine, which is further used to develop a novel colorimetric method for ultrasensitive and selective detection of KAN. The laccase-like catalytic center is formed through the multidentate chelation bonds between manganese ions and imidazolium groups of histidine as well as carboxyl groups of β-alanine in carnosine. The multivalent oxidation states of manganese dynamically regulate electron transfer pathways through synergistic coordination. The unique Mn-Car architecture drives the efficient generation of superoxide radicals (O 2 · − ) and semiquinone radicals (SQ· − ). Leveraging the excellent laccase-like activity of Mn-Car , a highly sensitive colorimetric method for KAN detection is developed. Mn-Car forms polydentate chelation with the amino and hydroxyl groups in KAN, which can trigger multi-step cascade oxidation reactions that disrupt the structural core of Mn-Car and lead to reduced colorimetric signals. The colorimetric method achieves a limit of detection (LOD) of 0.102 μM and is successfully applied for on-site detection of KAN in water samples and honey, providing a novel platform for antibiotic residue analysis.
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MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Ruiwen Li,Hao Shen,Dandan Shi,Shaoning Yu,Guoqing Qian
Infections with multidrug-resistant bacteria (MDRB) result in high morbidity and mortality. Among the various methods currently used to detect MDRB, culture-based methods are reliable but usually time-consuming and laborious, and polymerase chain reaction is expensive or cumbersome to perform, making it difficult to meet detection needs. In this work, we developed an accurate, efficient, and convenient colorimetric aptasensor for the detection of MDRB through aptamer-mediated enhancement of FeCoMn-MOF-74 peroxidase mimetic activity. Using New Delhi metallo-β-lactamase-1 Klebsiella pneumoniae (NDM-1 KP) and Klebsiella pneumoniae carbapenemase 2 Escherichia coli (KPC-2 E. coli ) as models, the proposed colorimetric aptasensor demonstrated a wide linear range (10–10 8 CFU/mL for both) and low detection limits (7 and 9 CFU/mL for NDM-1 KP and KPC-2 E. coli, respectively) under optimized conditions. In addition, the aptasensor exhibited high specificity, excellent immunity to interference, and high stability. The utility of the constructed aptasensor was verified by spiking the assay in serum, peritoneal fluid, and cerebrospinal fluid with satisfactory recoveries, which not only confirmed the clinical utility of the aptasensor in contaminated specimens, but also revealed the capacity of FeCoMn-MOF-74 as a generalized biosensing platform for sensitive detection of other MDRB.
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危险品化学品经营许可证（带存储）