SENSORS AND ACTUATORS B-CHEMICAL
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2025
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IF 7.7
Jie Chen,Haishan Wu,Weiwei Zhang,Yun Huang,Jingcheng Zheng,Jiwei Shao,Yuwu Chi
The sensitive detection of glutathione (GSH) level is of great significance to disease diagnosis. However, commonly used fluorometry method usually suffers from limited sensitivity, potential interferences and inefficient detection. In this work, a smartphone-assisted and perovskite-based sensing platform for dual-readout detection of GSH was developed: the silica coated perovskite quantum dots (PQD@SiO 2 ) with a single particle level and high fluorescence efficiency were employed as fluorophore, which greatly avoids aggregation-caused quenching effect caused by multi-particles embedded in one SiO 2 shell, and the manganese dioxide (MnO 2 ) nanosheets were used as quencher. With the addition of GSH, the fluorescence intensity of PQD@SiO 2 -MnO 2 composites gradually enhances, while the color of solution turns from dark brown to light yellow under sunlight, which enables the dual-signal (fluorescence and colorimetric) and reliable detection of GSH level. In addition, the introduction of smartphones integrated image recording and data processing, further promotes the simple, visualized, cost-effective and real-time determination of GSH. The synthesis of PQD@SiO 2 at a single particle level, as well as the strategies of dual-mode and smartphone-assisted detection, holds the promising prospect in biosensing and chemical sensing.
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MOLECULAR PHARMACEUTICS
·
2025
·
IF 4.5
Liang Zhang,Ting Wang,Yunyi Shan,Weijie Zhang,Jing Yao,Hui Xiong
Triple-negative breast cancer (TNBC) is a highly aggressive subtype with limited therapeutic options due to its resistance to conventional targeted therapies. TNBC cells heavily rely on oxidative phosphorylation (OXPHOS), making mitochondrial electron transport chain (ETC) inhibition a promising therapeutic approach. However, existing ETC inhibitors pose severe cytotoxicity risks, highlighting the need for biocompatible alternatives. In this study, we develop an MgO-ICG suspension (MgO-ICG@S), utilizing magnesium oxide (MgO), biocompatible pharmaceutical excipients that are already in widespread use as both an ETC-blocking agent and a drug carrier for the photosensitizer indocyanine green (ICG). This approach enables a bidirectional ETC blockade by disrupting ETC electronic transmission and functionality while inducing a unique mitochondria-targeted drug delivery effect. Additionally, laser irradiation activates ICG, generating reactive oxygen species (ROS) that further sensitize tumor cells to ETC inhibition. The combination of ETC blockade and photodynamic therapy (PDT) creates a synergistic cycle of oxidative damage, amplifying antitumor effects and significantly improving therapeutic outcomes. This study offers a promising strategy for effective, targeted TNBC treatment and provides valuable insights into the development of biocompatible ETC-blocking agents for clinical applications.
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Scientific Reports
·
2025
·Vol. 15
·期 1
·1-10
·
IF 3.9
Shen Fangzhou,Lu Zhaoyang,Yan Kangli,Luo Kang,Pei Shuchen,Xiang Pu
This work reports a novel one-pot hydrothermal synthesis of N-CQDs using Solanum nigrum L . as sustainable carbon source and triethylamine as nitrogen provider. The resulting N-CQDs were systematically characterized with TEM, SEM, FT-IR, XPS, XRD, and UV–Visible. Significantly, the N-CQDs exhibited substantial antimicrobial efficacy against both S. aureus and E. coli bacterial strains, with minimum inhibitory concentration values of 1.2 mg/mL and 1.1 mg/mL, respectively. Moreover, the N-CQDs demonstrated pronounced fluorescence enhancement upon interaction with ciprofloxacin, displaying exceptional selectivity and sensitivity, with a calculated LOD of 316 nM. The feasibility of employing N-CQDs as fluorescent probes for ciprofloxacin detection was systematically validated through practical application in honey analysis. This study introduces a novel approach for the fabrication of N-CQDs derived from natural botanical sources, underscoring their potential dual functionality as fluorescent sensors and antimicrobial materials.
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JOURNAL OF MOLECULAR STRUCTURE
·
2024
·
IF 4
Zi-Ping Wang,Yu Wang,Xin-Yu Li,Lin-Fang Jia,Ai-Zhen Yang,Wen-Ting Zhao,Yi Jia,Bao-Yi Yu,Han-Qing Zhao
The synthesis and characterization of three 2D to 3D metal-organic frameworks (MOFs) with mixed ligands, namely {[Cd(DINT)(TPA)(H 2 O)]ACN} n (BAO24) (H 2 TPA = tere phthalic acid, DINT = 1,4-di(imidazol-1-yl)naphthalene, and ACN = acetonitrile), {[Cd(DINT)(IPA)]DMF} n (BAO25) (H 2 IPA = iso phthalic acid and DMF = N,N -dimethylformamide), and [Cd(DINT)(PA)] n (BAO26) (H 2 PA = phthalic acid), were reported herein. The structures and purities of the MOFs were revealed and confirmed by single-crystal X-Ray diffraction, Fourier transform infrared spectroscopy, powder X-Ray diffraction, thermogravimetric analysis, and elemental analysis. The MOFs exhibited a fluorescence emission peak around λ em = 340 nm with relative excitation wavelengths at both 230 and 290 nm. Since the MOFs showed similar fluorescence properties, BAO24 was selected as a representative MOF in the fluorescent sensing processes. The result showed that BAO24 exhibited a high sensitivity for vitamin B2. However, when sensing pesticides, the sensor performed differently at each excitation wavelength. At λ ex = 230 nm, the sensor was quenched the most significantly with the nitenpyram analyte, while at λ ex = 290 nm, the highest fluorescence quenching was achieved by the imidacloprid analyte.
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ANALYTICAL CHEMISTRY
·
2025
·
IF 6.7
Yaqin Wang,Huiping Yang,Zhaoquan Li,Qian Mo,Shuang Li,Longda Li,Fan Yang,Xinchun Li,Yong Huang
Mimicking ion-gating function with artificial nanopores empowered by well-designed responsive DNA architectures represents one of the leading maneuvers in the nanopore sensing field. However, due to the rarely preponderant orientation and uncontrollable assembly of conventional DNA constructs at heterogeneous interfaces, the recognition ability and gating efficiency remain a considerable challenge. Here, we show that manipulating the ion-gating efficiency can be readily achieved by the assembly of responsive tetrahedral DNA nanostructures in glass nanopipettes. We design a set of 3D-DNA nanostructures consisting of size-tunable DNA tetrahedrons and Pb2+-dependent DNAzyme structural domain in the nanopipettes, which served as the ion gate and recognition element, respectively, and thus enabled the sensing of Pb2+ in a label-free manner. Using the constructed 3D-DNA-nanostructured ion-gating systems, we can program stimulus–response capacity and ionic current rectification behaviors in the Pb2+ sensing events. We found that larger-size DNA tetrahedrons resulted in more efficient ion gating and importantly could achieve dynamic linear response to Pb2+, with the concentration spanning from 10 pM to 10 μM. Finite element simulations revealed that steric hindrance, rather than surface charge, may be a principal cause for regulating trans-pore ion transport. This work opens an avenue for the design of DNA nanostructure-based responsive nanopores for sensing applications.
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POULTRY SCIENCE
·
2025
·
IF 4.2
Elham Ghashghaei,Minghui Wang,Ahmed Mijiyawa,Hai Lin
Amino acids in the brain modulate eating behavior and energy balance. The aim of the present study was to investigate the effects of DL-methionine (DL-Met), L-lysine monohydrochloride (L-Lys-HCl), and taurine (Tau) on feed intake and the mRNA expression levels of appetite-related hypothalamic neuropeptides in chicks. DL-Met, L-Lys-HCl, or Tau was intracerebroventricularly (ICV) administered to 5-day-old layer-type chicks, and the feed intake was recorded until 1 h post-injection. Quantitative PCR was performed to determine the hypothalamic mRNA expression levels of neuropeptide Y ( NPY ) and its receptors, agouti-related protein ( AgRP ), pro-opiomelanocortin ( POMC ), cocaine- and amphetamine-regulated transcript ( CART ), and melanocortin receptors. Our results indicated that injection time and treatment have a significant effect on food intake. Food intake decreased significantly at 30 and 60 minutes after ICV administration of DL-Met (1.678 μmol), L-Lys-HCl (6.856 μmol), and Tau (5.006 μmol) compared to the control group ( P > 0.05). On the contrary, a high dose of each amino acid did not alter feed intake ( P > 0.05). The result showed that ICV injection of DL-Met (1.678 μmol) significantly decreased the mRNA expressions of NPY , NPY4R , and NPY6R ( P < 0.05), but had no significant effect on the mRNA expressions of NPY1R, NPY5R, and AgRP in the hypothalamus ( P > 0.05). Similarly, ICV injection of L-Lys-HCl (6.856 μmol) and Tau (5.006 μmol) significantly decreased mRNA levels of NPY, NPY4R, and NPY6R ( P < 0.05), without affecting the mRNA expressions of NPY1R, NPY5R, and AgRP ( P > 0.05). DL-Met had no effect on anorexigenic gene expression ( P > 0.05). L-Lys-HCl upregulated MC1R ( P < 0.05), and Tau upregulated POMC and MC3R ( P < 0.05), and both had no influence on CART, MC4R , or MC5R ( P > 0.05). The result suggests that both orexigenic and anorexigenic neuropeptide genes are involved in the effects of DL-Met, L-Lys-HCl, and Tau. The effect of DL-Met, L-Lys-HCl, and Tau acts in a dose-dependent manner in the hypothalamus to influence feed intake.
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MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Siyu Wu,Xiaojuan Niu,Jiali Chen,Wangni Meng,Han Tao,Jianli Zhou,Yuangen Wu
Kanamycin (KAN) can generate a serious threat to human health due to its tendency to accumulate in food. Current colorimetric strategy for KAN detection still faces some challenges, and nanozyme-based colorimetry is an ideal alternative approach. Herein, a non‑copper-based multivalent laccase mimic named Mn-Car is first synthesized through a self-assembly of manganese ions and the endogenous biomolecule carnosine, which is further used to develop a novel colorimetric method for ultrasensitive and selective detection of KAN. The laccase-like catalytic center is formed through the multidentate chelation bonds between manganese ions and imidazolium groups of histidine as well as carboxyl groups of β-alanine in carnosine. The multivalent oxidation states of manganese dynamically regulate electron transfer pathways through synergistic coordination. The unique Mn-Car architecture drives the efficient generation of superoxide radicals (O 2 · − ) and semiquinone radicals (SQ· − ). Leveraging the excellent laccase-like activity of Mn-Car , a highly sensitive colorimetric method for KAN detection is developed. Mn-Car forms polydentate chelation with the amino and hydroxyl groups in KAN, which can trigger multi-step cascade oxidation reactions that disrupt the structural core of Mn-Car and lead to reduced colorimetric signals. The colorimetric method achieves a limit of detection (LOD) of 0.102 μM and is successfully applied for on-site detection of KAN in water samples and honey, providing a novel platform for antibiotic residue analysis.
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aBIOTECH
·
2025
·
IF 5
Zhang Wenyang,Wang Yanjiao,Li Wenyan,Wu Shaowen,Chen Yuanyuan,Ye Mingyang,Huang Wenjie,Fernie Alisdair R.,Yan Shijuan
Protein post-translational modifications such as phosphorylation and S -nitrosylation regulate protein functions and cellular programs in eukaryotes. Moreover, extensive evidence suggests crosstalk between these modifications. However, we lack a comprehensive method for the simultaneous detection and analysis of multiple post-translational modifications. Here, we present an optimized workflow that identifies phosphorylation and S -nitrosylation sites using a novel phosphate affinity tag switch technique. Validation with model proteins and complex biological samples confirmed the high sensitivity, coverage, and reproducibility of this method. Applying this method to Arabidopsis thaliana seedlings revealed 12,552 phosphorylation sites and 6,108 S -nitrosylation sites, representing the largest single-study dataset of S -nitrosylation sites to date. This approach enhances our understanding of post-translational modification dynamics in plant signaling, stress responses, and metabolism.
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MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Ruiwen Li,Hao Shen,Dandan Shi,Shaoning Yu,Guoqing Qian
Infections with multidrug-resistant bacteria (MDRB) result in high morbidity and mortality. Among the various methods currently used to detect MDRB, culture-based methods are reliable but usually time-consuming and laborious, and polymerase chain reaction is expensive or cumbersome to perform, making it difficult to meet detection needs. In this work, we developed an accurate, efficient, and convenient colorimetric aptasensor for the detection of MDRB through aptamer-mediated enhancement of FeCoMn-MOF-74 peroxidase mimetic activity. Using New Delhi metallo-β-lactamase-1 Klebsiella pneumoniae (NDM-1 KP) and Klebsiella pneumoniae carbapenemase 2 Escherichia coli (KPC-2 E. coli ) as models, the proposed colorimetric aptasensor demonstrated a wide linear range (10–10 8 CFU/mL for both) and low detection limits (7 and 9 CFU/mL for NDM-1 KP and KPC-2 E. coli, respectively) under optimized conditions. In addition, the aptasensor exhibited high specificity, excellent immunity to interference, and high stability. The utility of the constructed aptasensor was verified by spiking the assay in serum, peritoneal fluid, and cerebrospinal fluid with satisfactory recoveries, which not only confirmed the clinical utility of the aptasensor in contaminated specimens, but also revealed the capacity of FeCoMn-MOF-74 as a generalized biosensing platform for sensitive detection of other MDRB.
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MOLECULES
·
2025
·Vol. 30
·期 14
·2940
·
IF 4.6
Liang Chen,Qiao Wu,Chen Yang,Xiulan Xin,Zhaochu Xu,Shuai Luo,Hao Liang
Natural products possess potent pharmacological activities and health benefits. However, drawbacks such as water insolubility, poor stability, and low bioavailability limit their practical applications. This research is dedicated to the development of suitable natural self-assembled nano-delivery systems to encapsulate natural molecule drugs, improving their dispersion and stability in aqueous solution. As a model drug, curcumin (Cur) was encapsulated in zinc–adenine nanoparticles (Zn–Adenine), based on the self-assembly of a coordination matrix material. Hyaluronic acid (HA) was further functionalized on the surface of Cur@(Zn–Adenine) to realize a tumor-targeted delivery system. The morphology was characterized through TEM and zeta potential analyses, while the encapsulation mechanism of the nanoparticles was researched via XRD and FTIR. The formed Cur@(Zn–Adenine)@HA nanoparticles exhibited good drug loading efficiency and drug loading rate. Moreover, compared to free Cur, Cur-loaded (Zn–Adenine)@HA showed enhanced pH stability and thermal stability. In particular, Cur@(Zn–Adenine)@HA demonstrated excellent biocompatibility and strong specificity for targeting CD44 protein on cancer cells. The above results indicate that (Zn–Adenine)@HA NPs can serve as an effective nano-delivery system for hydrophobic substances.
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CANCER CELL
·
2025
·
IF 44.5
Yang Yang,Chuanyan Yang,Xuezhu Chen,Yibin Jiang,Xuejiao Lei,Kang Ma,Yulian Quan,Tianran Li,Chenfu Guo,Yijing Meng,Lin Kang,Xinyu Zhang,Long Jin,Jiafeng Huang,Ning Mu,Zexuan Yan,Qinghua Ma,Shuai Wang,Yanxia Wang,Yong-Ning Shang,Cong Chen,Yu Shi,Shukun Hu,Likun Yang,Chuan Lan,Rong Hu,Ying Zhang,Xia Li,Yunqing Li,Chong Liu,Yu-Hai Wang,Fei Li,Hua Feng,Xiu-Wu Bian,Tunan Chen
Glioblastoma (GBM), the most aggressive primary brain tumor, is shaped by its integration into neural networks. While glutamatergic input is linked to tumor progression, the broader architecture and function of neuron-glioma connectomes remain unclear. Using monosynaptic rabies tracing, we map brain-wide neural input to patient-derived xenografts and reveal a consistent organizational logic: local inputs are primarily glutamatergic, while long-range connections exhibit diverse neurotransmitter profiles, with basal forebrain cholinergic projections emerging as a conserved input across sites. Functionally, presynaptic acetylcholine release promotes GBM progression through muscarinic receptor CHRM3 in a circuit-specific manner. Mechanistically, glutamatergic and cholinergic signals converge to enhance glioma calcium transients but diverge in temporal transcriptional control, with their dual blockade producing additive anti-tumor effects. Therapeutically, the anticholinergic drug scopolamine attenuates glioma growth, whereas the acetylcholinesterase inhibitor donepezil exacerbates disease. These findings reveal the complexity of neuron-glioma connectivity, highlighting long-range neuromodulatory pathways as promising therapeutic targets in GBM.
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Biomaterials Advances
·
2025
·
IF 6
Meng Lan,Fansu Meng,Lanwen Gao,Anil K. Giri,Makiya Nishikawa,Kosuke Kusamori,Jaiwoo Lee,Mulazim Hussain Asim,Shumaila Arshad,Honghui Gu,Qi Li,Lina Yang,Zhong Chen,Zhenjiang Yang,Jiajia Qin,Yu Cai
Background Triple-negative breast cancer (TNBC) faces great challenges in clinical treatment, owing to the lack of specific therapeutic targets and easy metastasis. The natural component baicalin can effectively inhibit the growth and metastasis of TNBC; however, it has some limitations, such as poor targeting and side effects. Nano targeted delivery systems can improve drug efficacy by enhancing drug accumulation and controlling drug release. Objective Trop-2 transmembrane glycoprotein expression is high in TNBC cells, suggesting that it can serve as a specific active targeting molecular-modified nano drug delivery system for TNBC to overcome non-specific distribution. Based on the characteristics of high-concentration glutathione in the tumor microenvironment, redox-sensitive nano-prodrugs (Trop2-BA-ss-PPEP) have been designed to achieve intelligent slow control and release of drugs. Methods The chemical structure of the Trop2-BA-ss-PPEP, and its stability, reductive response to drug release behavior, and targeting ability in vitro were characterized. Cell experiments and a transplanted tumor model verified the anti-tumor effect and biosafety. Results Trop2-BA-ss-PPEP was stable in a physiological environment and rapidly released the drug under reducing conditions. The experiments showed that Trop2-BA-ss-PPEP significantly promoted cellular uptake, and drug accumulation and maintenance time at the tumor site were increased. It enhanced the inhibitory effect on metastasis in vivo and in vitro, and no obvious toxicity or side effects were observed. Conclusion Trop2-BA-ss-PPEP was successfully constructed. The targeting ability, microenvironment responsiveness, and anti-tumor metastatic effects of Trop2-BA-ss-PPEP provide a new strategy for TNBC therapy, which has good application and transformation potential.
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