LWT-FOOD SCIENCE AND TECHNOLOGY
·
2021
·
IF 4.952
Zhiyu Xiong,Tong Shi,Wei Zhang,Yunfei Kong,Li Yuan,Ruichang Gao
The combined effects of l -arginine (Arg) and oxidized caffeic acid (OCF) treatment under different salt concentrations on gelling properties of bighead carp ( Aristichthys nobilis ) surimi gels were investigated. The results showed that the improvement of gel strength and water-holding capacity (WHC) of low-salt (0.5%) surimi gels could be obtained by adding Arg at various levels (0.1–0.4%) rather than OCF (0.1–0.9%, based on protein content). However, the gel strength and WHC of the low-salt surimi gel added with low-dose (0.1%) Arg alone were still far lower than that of regular-salt (3.0%) control. Whereas the significant improvements in low-salt surimi gels could be achieved when 0.1% Arg was combined with various levels of OCF, especially in combination with 0.5% OCF (LC-A-O). The water distribution in LC-A-O was markedly improved and its β-sheet structure content was significantly increased compared with the low-salt control ( P < 0.05). Moreover, a denser and more uniform network structure of LC-A-O was obtained, which was probably due to much stronger and more intra-molecular cross-linking of proteins, via the formation of new adducts between OCF and surimi proteins. Hence, the combined treatment of Arg and OCF showed a potential for improving the gel properties of low-salt surimi.
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高影响因子
POLYMER INTERNATIONAL
·
2021
·Vol. 70
·期 12
·1717-1724
·
IF 2.99
Lei Wang,Yifan Huang,Binjie Xin,Tingxiao Li
Electrospun core–shell nanofibers have aroused attention in drug delivery systems and tissue engineering because of the latent structures which can encapsulate various drugs and obtain tailored release profiles. Here, poly( l -lactide- co -ε-caprolactone) (PLCL) with gelatin (GE) blends were employed to electrospin the shell of core–shell nanofibers which could act as a protection layer for the controlled released behavior of the model drug doxorubicin hydrochloride (DOX). Core–shell nanofiber membranes with different drug loaded amounts were fabricated and the corresponding drug release behaviors were researched. The surface morphology of the obtained nanofibers was assessed by SEM and the structure of the core–shell nanofibers were observed by inverted fluorescence microscopy and TEM. The hydrophilicity was evaluated by water contact angle testing and the results indicated that the obtained fibers all possess good hydrophilicity. The mechanical properties of the fiber membranes were evaluated by tensile testing. Exploring the composition of the fiber membranes by Fourier transform infrared spectroscopy proved that DOX existed in the drug-loaded fiber membranes. In vitro release was evaluated by UV–visible spectroscopy and the accumulative release curves of DOX showed that increasing drug rates would lead to increasing cumulative release amounts but decreasing cumulative drug release rates. The results indicated that drugs encapsulated in PLCL/GE core–shell nanofiber material would be efficient and a potential material for drug delivery systems. © 2021 Society of Chemical Industry
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高影响因子
FlatChem
·
2020
·
IF 0
Yang Xia,Kaiqian Wang,Ying Shi,Xingcai Gui,Chaonan Lv,Haisheng Tao
With the social and economic development, environmental pollution issues have gradually become a serious threat to ecosystem. Therefore, effective detection methods of environmental pollutants have been widely expanded. In this study, an electrochemical sensor based on a functioned graphene was constructed. The functional graphene was obtained by electrochemical deposition of L-cysteine on graphene oxide modified glassy carbon electrode. The composite on the modified glassy carbon electrode was characterized by scanning electron microscopy, transmission electron microscopy and X-ray photoelectron spectroscopy. The characterized results discovered that graphene oxide was reduced and cross-linked to L-Cys simultaneously, with a corrugated sheet structure. The electrochemical behavior of the electrode was investigated by electrochemical impedance spectroscopy and cyclic voltammetry, and proved that the electrode has a rapid transfer of electrons and a good catalytic effect on environmental pollutant of hydroquinone. The constructed electrode can be used to detect hydroquinone, with the detection limit of 1.5 μM (S/N = 3), and a good linear relationship in the range of 2–160 μM. The low relative standard deviation of identical sensors and good anti-interference for coexisting matters revealed that the sensor has an important theoretical significance and broad application prospect.
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DOI
ANALYST
·
2020
·Vol. 146
·期 3
·956-963
·
IF 3.978
Jun Ma,Gege Feng,Ying Ying,Yong Shao,Yongxin She,Lufei Zheng,A. M. Abd EI-Aty,Jing Wang
Herein, an indirect SERS sensing assay was developed for the determination of glyphosate (Gly) in tap water. The mechanism of detection was based on relieving the inhibitory effect of L-cysteine (L-cys) on a Au–Pt nanozyme by combining Gly with L-cys through divalent copper ions (Cu2+). In this method, a novel nano-chain-like Au–Ag composite with good repeatability was successfully fabricated to detect SERS signals of oxTMB without disturbing TMB Raman signals. Under optimal conditions, the SERS signal intensity of oxTMB (at 1605 cm−1) was proportional to the concentration of Gly. The results showed a good linear response over the concentration ranges of 10 μg L−1 to 1000 mg L−1. The limit of detection and limit of quantitation of Gly were found to be 5 μg L−1 and 10 μg L−1, respectively. In addition, good anti-interference ability against interfering cations and structural analogues deserves to be mentioned. This SERS assay can be used for detection of Gly in tap water that can meet the needs of practical detection.
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PubMed
高影响因子
GENERAL AND COMPARATIVE ENDOCRINOLOGY
·
2020
·
IF 2.428
Liping Yang,Shaoyang Zhi,Guokun Yang,Chaobin Qin,Wenli Zhao,Mingming Niu,Wenlei Zhang,Wenyu Tang,Xiao Yan,Yuru Zhang,Xiaolin Meng,Ronghua Lu,Guoxing Nie
Irisin, encoded by fibronectin type III domain-containing protein 5 (FNDC5) gene, plays a role in energy expenditure and insulin sensitivity in mice. In fish, the function of irisin related to glucose metabolism is less reported. It may increase glucose utilization in fish. The aim of the present study was to characterize the regulatory role of irisin in glucose metabolism in common carp ( Cyprinus carpio L.). In this study, FNDC5a and FNDC5b were isolated from common carp. The cDNA of FNDC5a and FNDC5b were 722 bp and 714 bp, encoding 221 and 207 amino acids, respectively. FNDC5a was abundantly expressed in the brain and gonad. FNDC5b was mainly expressed in brain. Different expression pattern of FNDC5a and FNDC5b under fasting/refeeding and OGTT experiment were identified. The recombinant common carp irisinA and irisinB were prepared by prokaryotic expression system. Glucose concentration was decreased in treatment with irisinA or irisinB in the in vitro and in vivo experiments. The mRNA expression levels of gluconeogenesis-related genes were significantly down-regulated, while the mRNA expression of glycolysis-related genes were significantly up-regulated after treatment with recombinant irisinA or irisinB in liver in vivo and in primary hepatocytes in vitro. Our research shows that irisin inhibits hepatic gluconeogenesis and promotes hepatic glycolysis. Taken together, this study for the first time revealed the two subtypes of FNDC5 and explored the function and mechanisms of irisinA and irisinB in fish glucose homeostasis.
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PubMed
高影响因子
CARBOHYDRATE POLYMERS
·
2020
·
IF 7.182
Siyi Li,Na Liang,Pengfei Yan,Yoshiaki Kawashima,Shaoping Sun
Insulin is the most effective drug in the treatment of diabetes mellitus. At present, subcutaneous injection is still the common way for insulin delivery. However, oral delivery is considered as the most preferred way for its high patient compliance and the minimal invasiveness. In this study, a novel N -acetyl- L -cysteine and arginine modified hydroxypropyl-β-cyclodextrin (NAC-HP-β-CD-Arg) was successfully synthesized and characterized. The polymer was used as a carrier for oral delivery of insulin by forming NAC-HP-β-CD-Arg@insulin complex. Enzymatic degradation study indicated that the NAC-HP-β-CD-Arg could protect insulin from enzymolysis. Moreover, the polymer exhibited strong binding ability with mucin. The transportation efficiency of NAC-HP-β-CD-Arg@insulin across the Caco-2 cell monolayer was much greater than free insulin. The in vivo study demonstrated that the orally administered NAC-HP-β-CD-Arg@insulin exhibited an excellent and sustained hypoglycemic effect in diabetic rats. It can be concluded that the NAC-HP-β-CD-Arg is a potential carrier for oral delivery of insulin.
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PubMed
高影响因子
BIOMACROMOLECULES
·
2021
·
IF 6.988
Shuai Zhu,Ruizhong Xue,Zikun Yu,Xu Zhang,Shifang Luan,Haoyu Tang
Poly(l-cysteine)s with methylthio pendants (PMTLCs) were synthesized by ring-opening polymerization of a new l-cysteine-based N-carboxyanhydride. The thioether bonds of PMTLC can be readily oxidized by H2O2 yielding water-soluble PMTLCOX. The methylthio groups can undergo an alkylation reaction using methyl iodide and a subsequent ion-exchange reaction yielding sulfonium-based polypeptides (PPLC-DMS-X, where X = I, BF4). PPLC-DMS-X showed upper critical solution temperature-type thermo- and oxidation-responsive properties in aqueous solutions. Both PMTLC and PPLC-DMS-X showed oxidation-induced β-sheet to α-helix transitions. The absorbance of PPLC-DMS-I and methyl orange aqueous solution displayed a significant linear correlation with temperature, which makes the sulfonium-based polypeptides good candidates in the field of temperature sensors.
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PubMed
高影响因子
NANO
·
2021
·
IF 1.556
Chengxiang Chu,Rui Huang,Xianfeng Wang,Yuanyi Deng,Tao Gu,Danqun Huo,Liang Zhang,Daidi Zhong,Changjun Hou
As an important amino acid in the body, L-histidine participates in various physiological processes. Therefore, it is of great necessity to establish a rapid and effective method for L-histidine sensing, especially in blood and urine. In this work, a fluorescence sensor for detection of L-histidine was successfully developed using Cu-MOFs/Fe 3 + sensor. Cu-MOFs with blue emission served as the identification unit and signal source, and Fe 3 + played the role of “quencher” in this system. Fe 3 + induced the fluorescence quenching of Cu-MOFs to form Cu-MOFs/Fe 3 + sensor due to the effect of photoinduced electron transfer (PET). After the addition of L-histidine, Fe 3 + was captured by L-histidine to form L-histidine-Fe 3 + complex, which inhibited the PET effect from Cu-MOFs to Fe 3 + , resulting in the fluorescence recovery of Cu-MOFs. With such a design, L-histidine detection was realized with a wide linear range from 1 μ M to 190 μ M. The limit of detection (LOD) was calculated as low as 0.156 μ M. In addition, successful detection of L-histidine in blood serum with high selectivity and sensitivity indicated the real applications and potential diagnostic value of Cu-MOFs/Fe 3 + system.
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高影响因子
BIORESOURCE TECHNOLOGY
·
2021
·
IF 9.642
Yu Zhang,Minhua Wei,Guihong Zhao,Wenjie Zhang,Yingzi Li,Beibei Lin,Yanjun Li,Qingyang Xu,Ning Chen,Chenglin Zhang
l -Homoserine is a valuable non-proteinogenic amino acid used in the synthesis of various important compounds. Microbial fermentation has potential value for producing l -homoserine on a large scale, but suffers from a low yield and the need for expensive additives. In this study, a non-induced, non-auxotrophic, plasmid-free Escherichia coli chassis for the high-efficiency production of l -homoserine was constructed. Initially, the l -homoserine degradation pathway was dynamically attenuated. Subsequently, systems metabolic engineering strategies were employed, including reinforcing the synthetic flux, improving NADPH generation, and elevating l -homoserine efflux. The constructed strain HOM-14, produced 60.1 g/L l -homoserine without additional supplements or inducers, which achieved the highest fermentative production efficiency of l -homoserine till date. Moreover, common byproducts, such as acetate, did not accumulate. The strategies presented here can be applied in the further engineering of chassis for the scale-up production of l -homoserine and derivatives.
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PubMed
高影响因子
POLYMER
·
2020
·
IF 4.231
Xu Yang,Baolong Wang,Di Sha,Yonggang Liu,Jiuduo Xu,Kai Shi,Cong Yu,Xiangling Ji
A series of injectable and antibacterial hydrogels based on ε-poly ( l -lysine)-modified poly (vinyl alcohol) (CPVA- g -EPL)/chitosan (CS)/Ag complexes was fabricated using oxidized dextran as a crosslinker. As-prepared hydrogels CPVA- g -EPL/CS exhibited swelling ratios of approximately 33.9–55.4 g g −1 and macroporous structure with average pore size of 54–102 μm in dry state. The concentration of bacteria ( Escherichia. coli and Staphylococcus. aureus ) after treating with hydrogels for 4 h decreased by four orders of magnitude compared with that after blank control treatment, indicating excellent antibacterial property. After adding Ag nanoparticles (AgNPs), the antibacterial activities of hydrogels (i.e., CPVA- g -EPL/CS/AgNPs) were further enhanced. Moreover, the prepared hydrogels possessed good biocompatibility against the mouse fibroblast. In a mouse skin defect model, histological examination showed increased thickness, integrity of the epidermal tissue and formation of the collagen fibers, implying that these hydrogels remarkably accelerated the wound healing process. Thus, this kind of injectable hydrogel can be a good candidate as wound healing dressings applied in wound therapy.
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高影响因子
POLYMER BULLETIN
·
2021
·Vol. 79
·期 3
·1615-1626
·
IF 2.87
Yang Rui,Hao Boran,Zhang Kan
The cleavage of the oxazine ring of benzoxazine resins generally requires high energy during the ring-opening polymerization, which leads them to be processed at a much higher temperature than the traditional thermoplastics. In this paper, a bio-based substance, L-histidine, has been used as the catalyst to lower the ring-opening polymerization of the well-commercialized benzoxazine resin (BA-a) based on aniline and bisphenol-A. The catalytic effect of L-histidine on the polymerization behaviors of benzoxazine resin (BA-a) has been monitored by differential scanning calorimetry and in situ Fourier transform infrared spectroscopy. In addition, L-histidine has also been found to be a good property modifier for BA-a-derived thermosets. The thermal properties of resulting polybenzoxazines have been investigated by thermogravimetric analysis and dynamic thermomechanical analysis. Notably, the thermoset derived from BA-a with adding only 5 mol% L-histidine exhibits excellent thermal stability in consideration of high T g (206 °C), high T d10 temperature (319 °C) and high chair yield value (40%), making this thermosetting resin a promising material for high-performance applications.
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高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2020
·
IF 4.006
Mengting Zhu,You Long,Yingjie Ma,Yi Chen,Qiang Yu,Jianhua Xie,Bin Li,Jinlong Tian
This study aimed to quantitatively screen and compare the chemical compounds including protein, lipid, sucrose , total phenolics (TPC), and total titratable acidity (TTA) along with the fatty acid profile of green coffee beans from various geographical origins (China, Indonesia, Ethiopia, Kenya, Guatemala, Honduras, Brazil, and Colombia). Meanwhile, partial least squares discriminant analysis (PLS-DA) was used to investigate whether the analyzed parameters’ data can be successfully applied to differentiate the geographical origins of green coffee beans. The levels of protein, lipid, sucrose , TTA, and TPC in all samples were in the range of 13.06–15.98 g 100 g −1 , 12.88–16.29 g 100 g −1 , 7.30–11.45 g 100 g −1 , 231.43–361.21 mL 0.1 mol L −1 NaOH 100 g −1 , and 37.56–50.57 mg GAE g −1 , respectively. The fatty acid composition was dominated by C 18:2 , C 16:0 , C 18:1, and C 18:0 . The major fatty acid group was unsaturated fatty acids (USFA), accounting for 51.66–54.28% of total fatty acids. Statistical analysis showed significant differences in the chemical and fatty acid composition of green coffee beans from eight geographical origins ( P ＜ 0.05 ). Moreover, the PLS-DA results revealed excellent discrimination between the different geographical origins of green coffee beans, highlighting lipid, C 24:0 , C 22:0 , C 18:3 , C 17:0 , C 18:0 , C 20:0 , C 16:0 , and protein as discriminatory features.
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高影响因子
危险品化学品经营许可证（带存储）