查找使用阿拉丁产品的科研文献

Torachrysone-8-O-β-d-glucoside mediates anti-inflammatory effects by blocking aldose reductase-catalyzed metabolism of lipid peroxidation products

Wen-long Zhao,Di Xu,Jun-song Wang

Aldose reductase (AR) is an important enzyme involved in the reduction of various aldehyde and carbonyl compounds, including the highly reactive and toxic 4-hydroxynonenal (4-HNE), which has been linked to the progression of various pathologies such as atherosclerosis , hyperglycemia , inflammation, and tumors. AR inhibitors have potential therapeutic benefits for these diseases by reducing lipid peroxidation and mitigating the harmful effects of reactive aldehydes. In this study, we found that torachrysone-8- O -β- d -glucoside (TG), a natural product isolated from Polygonum multiflorum Thunb., functions as an effective inhibitor of AR, exhibiting potent effects in clearing reactive aldehydes and reducing inflammation. TG up-regulated the mRNA levels of several antioxidant factors downstream of NRF2, especially glutathione S-transferase (GST), which is significantly increased, thus detoxifying 4-HNE by facilitating the conjugation of 4-HNE to glutathione, forming glutathione-4-hydroxynonenal (GS-HNE). By employing a combination of molecular docking, cellular thermal shift assay, and enzyme activity experiments, we demonstrated that TG exhibited strong binding affinity with AR and inhibited its activity and blocked the conversion of GS-HNE to glutathionyl-1,4-dihydroxynonene (GS-DHN), thereby preventing the formation of protein adducts and inducing severe cellular damage. This study provides novel insights into the anti-inflammatory mechanisms of AR inhibitors and offers potential avenues for developing therapeutic strategies for AR-related pathologies. Our findings suggest that TG, as an AR inhibitor, may hold promise as a therapeutic agent for treating conditions characterized by excessive lipid peroxidation and inflammation. Further investigations are needed to fully explore the clinical potential of TG and evaluate its efficacy in the treatment and management of these complex diseases.

Separation and concentration of o–toluidine and tricyclazole from water with micellar enhanced ultrafiltration based on sodium dodecyl sulfate surfactant

Peng Wu,Yiqing Liu,Yongsheng Fu,Shixiang Wang,Hongbin Wang,Jianming Zhou

Spherical nanoflower-like bimetallic (Mo,Ni)(S,O)3-x sulfo-oxide catalysts for efficient hydrogen evolution under visible light

Xiaoyun Chen,Huizhi Sun,Dong-Hau Kuo,Adugna Boke Abdeta,Osman Ahmed Zelekew,Yuanbo Guo,Jubin Zhang,Zhanhui Yuan,Jinguo Lin

Photocatalytic H 2 O splitting by sulfide-based materials is a great challenge, because of the poor resilience of such materials against hole oxidation. Although sulfide ion of catalyst negatively shifts the valence band-edge relative to its oxide ion, the instability of sulfide ions during H 2 O oxidation is a critical obstacle to simultaneous evolution of H 2 and O 2 . Here, active, stable, and spherical nanoflower-like bimetal (Mo,Ni)(S,O) 3- x sulfo-oxide catalysts with a band gap of ∼2.1 eV and different concentrations of oxygen vacancy defects were synthesized for H 2 O splitting. (Mo,Ni)(S,O) 3- x of 25 mg with a suitable amount of oxygen vacancy defects could evolve 587.5 μmol/h H 2 under visible-light irradiation. This work demonstrated an example of converting an oxidation photocatalyst into a reduction one. Microstructure analysis showed that surface oxygen vacancy defects and the multiple-valence charges in Ni and Mo not only promoted effective separation, interface transfer, and reactions of photo-carriers but also reduced the charge build-up to avoid photo-corrosion during photocatalytic water decomposition.

Assembling cyanidin-3-O-glucoside by using low-viscosity alginate to improve its in vitro bioaccessibility and in vivo bioavailability

Chao Zou,Ling Huang,Donghui Li,Yu Ma,Yixiang Liu,Yanbo Wang,Min-Jie Cao,Guang-Ming Liu,Lechang Sun

In this work, an enteric soluble alginate was proposed to improve the absorption efficiency of cyanidin-3-O-glucoside (C3G) through molecular self-assembly. Under the optimized conditions, the obtained low-viscosity alginate (LVA) was released completely during the simulated gastrointestinal digestion and an LVA–C3G complex with 84.2% binding efficiency was acquired. Fourier transform infrared spectroscopy displayed that the characteristic spectrum of C3G had disappeared after the LVA conjugation. Furthermore, based on the analysis of scanning electron microscopy and differential scanning calorimetry, a porous network structure and the shifted endothermic peak in the thermograms were observed, further confirming the formation of a complex between LVA and C3G. The results of simulated gastrointestinal digestion reveal that the LVA assembly significantly ( p  < 0.05) improved the bioaccessibility of C3G. Correspondingly, the C3G level in mouse plasma was increased by 27.4% in the C3G-LVA group. This suggests the suitability of LVA as an oral delivery vehicle for dietary anthocyanins.

Improve the physical and oxidative stability of O/W emulsions by moderate solidification of the oil phase by stearic acid

Yuehan Wu,Fangfang Chen,Chao Zhang,Wei Lu,Zhiming Gao,Longquan Xu,Ran Wang,Katsuyoshi Nishinari

Physical instability and oxidation were two of the most evident problems existing in food emulsions . In this research, a facile approach of solidifying the oil phase by stearic acid was adopted to overcome the above problems simultaneously, emphasising the effect of the oleogelation extent. Polarizing microscope, rheological analysis, and X-ray diffraction (XRD) were used to characterize the oleogelation of the oil phase, and droplet size, microscopic observation and peroxide value analysis were applied to evaluate the emulsion stability. Results showed that with moderate solidification of the oil phase (0.8%–1.0% w/v stearic acid in oil phase), the emulsions exhibited excellent physical stability under the stress of centrifugal force, high salt, and long-term storage. Moreover, the oxidative stability of the emulsions was improved simultaneously, that the increasing of peroxide value was slowed significantly. Excessive solidification (e. g. 3% w/v stearic acid) of the oil phase would weaken these advantages or even bring about opposite results, due to the destruction of the emulsion droplets by the excessive crystallization. Our results suggested that moderate solidification of oil phase would be a feasible and effective approach to improve the stability of o/w emulsions, which would be significant for the industrial-scaled production.

Metabolomics analysis reveals the protective effect of quercetin-3-O-galactoside (Hyperoside) on liver injury in mice induced by acetaminophen

Cheng Hu,Ying Chen,Yiyuan Cao,Yiqun Jia,Jiaqi Zhang

We investigated the protective effect of Hyperoside (HPS) on liver injury induced by acetaminophen (APAP) in C57 mice. HPS was administered orally for 7 days and APAP was administered orally on the 7th day. Serum and liver samples were then collected for biochemical analyses, histopathology assessments, and metabolomics studies. Metabolites were assessed using a UHPLC-MS system. Principal component analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA) were used to process the data. Pathway analyses were performed using Metaboanalyst 4.0. Western blot and qRT-PCR were used to determine the protein and mRNA levels, respectively. HPS interacted with active sites in CYP2E1 and caused protein degradation. In conclusion, our results suggested that HPS prevented the oxidative stress-induced liver injury caused by APAP. Practical applications Hyperoside was shown to have potential protective and therapeutic effects against liver diseases. Male C57 mice were used to perform pharmacodynamic, pharmacology, and metabolomics evaluations. At a dose of 60 mg/kg, HPS prevented oxidative stress-induced liver injury caused by APAP by regulating the glutathione-related metabolites and enzymes through the inhibition of CYP2E1.

Preparation of reduced Ni-Nb-O composite hydrogenation catalysts for highly selective conversion of free fatty acids to n-alkanes

Lei Chen,Guangci Li,Mingjie Zhang,Da Wang,Shuangju Li,Chuanhui Zhang,Xuebing Li,Keng H. Chung

Catalytic deoxygenation of fatty acids to alkanes is an ideal route to produce biodiesel with high heating value . However, traditional transition-metal sulfide and noble metal catalysts are sulfide-contaminated, high cost, or easily poisoned. To overcome the above issues, this work developed a non-noble and sulfide-free reduced transition-metal composite Ni x Nb y O z , which was effective to selectively hydrogenate stearic acid , a typical fatty acid model compound, to diesel-range alkanes. By using a simple slurry method, reduced Ni x Nb y O z catalysts have been successfully prepared with an outstanding catalytic performance. The results showed that catalyst Ni 0.5 Nb 0.5 O z effectively converted stearic acid to n -alkanes with nearly 100% conversion and >99% n -alkanes selectivity at mild conditions (513 K and 3.5 MPa of H 2 pressure). Appropriate Nb species improved the dispersion of Ni species, which may supply more hydrogenation active sites. Two possible reaction routes of stearic acid were proposed, and the hydrogenolysis of stearyl stearate was confirmed as the rate-determining step based on the kinetics experiments. Because of moderate acidity of Nb species, the ratio of C 17 /C 18 alkane was not markedly affected by reaction conditions when the conversion of stearic acid was higher than 99%. Moreover, catalyst Ni 0.5 Nb 0.5 O z showed a high recycle ability and compatibility for other long-chain fatty acids, demonstrating that catalyst Ni 0.5 Nb 0.5 O z is a promising green catalyst for producing biodiesel from fatty acids.

One-step production of N–O–P–S co-doped porous carbon from bean worms for supercapacitors with high performance

Zhentao Bian,Chunjie Wu,Chenglong Yuan,Ying Wang,Guangzhen Zhao,Hongyan Wang,Yong Xie,Cong Wang,Guang Zhu,Chong Chen

In recent years, multi-heteroatom-doped hierarchical porous carbons (HPCs) derived from natural potential precursors and synthesized in a simple, efficient and environmentally friendly manner have received extensive attention in many critical technology applications. Herein, bean worms (BWs), a pest in bean fields, were innovatively employed as a precursor via a one-step method to prepare N–O–P–S co-doped porous carbon materials. The pore structure and surface elemental composition of carbon can be modified by adjusting KOH dosage, exhibiting a high surface area (SBET) of 1967.1 m2 g−1 together with many surface functional groups. The BW-based electrodes for supercapacitors were shown to have a good capacitance of up to 371.8 F g−1 in 6 M KOH electrolyte at 0.1 A g−1, and good rate properties with 190 F g−1 at a high current density of 10 A g−1. Furthermore, a symmetric supercapacitor based on the optimal carbon material (BWPC1/3) was also assembled with a wide voltage window of 2.0 V, demonstrating satisfactory energy density (27.5 W h kg−1 at 200 W kg−1) and electrochemical cycling stability (97.1% retention at 10 A g−1 over 10 000 charge/discharge cycles). The facile strategy proposed in this work provides an attractive way to achieve high-efficiency and scalable production of biomass-derived HPCs for energy storage.

(-)-Syringaresinol-4-O-β-D-glucopyranoside from Cortex Albizziae inhibits corticosterone-induced PC12 cell apoptosis and relieves the associated dysfunction

Desen Yang,Wanqin Wu,Guoping Gan,Dingkun Wang,Jing Gong,Ke Fang,Fuer Lu

The neuroprotective effects and potential mechanisms of (-)-Syringaresinol-4-O-β-D-glucopyranoside (SRG), a natural lignan glycoside extracted from Cortex Albizziae , were investigated using corticosterone (CORT)-induced PC12 cells as an in vitro anxiety model. PC12 cells were treated with 100 μM CORT and 5, 10, or 20 μM SRG for 48 h. Cell viability and lactate dehydrogenase (LDH) leakage were measured. Apoptosis were detected using FITC-coupled Annexin V (AV) and propidium iodide (PI) staining flow cytometric analyses and TUNEL assays. Rhodamine 123 and Fluo-3-AM staining flow cytometric analyses were used to detect mitochondrial membrane potential (ΔΨm) and intracellular calcium concentration ([Ca 2+ ] i ), respectively. Western blot was used to detect brain-derived neurotrophic factor (BDNF), Bax, Bcl-2, cAMP-response element binding protein (CREB), cytosolic cytochrome c (Cyt c ), caspase-3, and cleaved caspase-3. Experimental data showed that SRG promoted cell proliferation , reduced LDH release, inhibited apoptosis, improved ΔΨm values, decreased [Ca 2+ ] i , up-regulated CREB, BDNF, and Bcl-2, down-regulated Bax and Cyt c protein expression levels, and reduced caspase-3 activity. This suggests that SRG has neuroprotective and antiapoptotic effects in the pathogenesis of anxiety disorders, and its mechanisms are partly connecte to inhibition of the mitochondrial apoptotic pathway and activation of pathways involving CREB and BDNF.

Effects of silver nanoparticle on electrochemical performances of poly(o-phenylenediamine)/Ag hybrid composite as anode of lithium-ion batteries

Su Chang,Han Bing,Ma Jiaojiao,Guo Pengju,Xu Lihuan

In the present work, the poly(o-phenylenediamine)/Ag (PoPD/Ag) hybrid composite with the microrod morphology was prepared by in situ chemical oxidation method, and then composite electrodes of PoPD/Ag (PVDF) and PoPD/Ag (sodium alginate) were prepared by using PVDF and sodium alginate as electrode binders, respectively. As explored as the anode of lithium ion batteries, the redox-active PoPD in the hybrid composite, compared to the pure PoPD, demonstrated the remarkably improved electrochemical performances with superior high capacity and good rate capability and cycling stability, due to the improved intrinsic electrical conductivity of PoPD via the introduced Ag nanoparticles in the polymer. As the sodium alginate was chosen as the functional electrode binder, PoPD/Ag (sodium alginate) exhibited the initial charge/discharge-specific capacity of 1452.9/2317.7 mAh g −1 , and after the 100th cycles, the developed discharge capacity for PoPD/Ag (sodium alginate) reached to 1389.9 mAh g −1 , which was evidently higher than that of PoPD. Its stable and representative discharge-specific capacities were 1291, 1044.9, 856.7, 762.5 mAh g −1 at the current rate of 50, 100, 200, and 500 mAh g −1 , respectively, which were obviously superior to that of PoPD and PoPD/Ag (PVDF), indicating that sodium alginate as binder has the special effects on the electrochemical characteristics of PoPD, which benefited to the capacity release step by step during the charge/discharge process. The works would provide significant exploration for the preparation of high-performance poly (aromatic amine) polymer as the electrode materials.

Porous carbonized cotton loaded with Zn–Cu–M(M=O, S) nanocomposites for electrochemical energy storage and oxygen evolution reaction

S. Zhai,Y. Luo,Z. Fan,M. Zhou,K. Hou,H. Zhao,M. Hu,Y. Xiao,K. Jin,Y. Zhao,X. Li,Z. Cai

A new strategy has been designed for enhancing the electrochemical performances of the supercapacitor and water-splitting electrodes. Hetero-structure composite electrode based on copper sulfide (CuS), zinc oxide (ZnO), and porous carbonized cotton (PCc) is prepared via vacuum thermal evaporation technology and successive ionic layer adsorption methods. Porous substrate provides plenty of active sites, which facilitates the exchange of ions/charges in the electrode processes. The composite electrode exhibits outstanding electrochemical energy storage and electrocatalytic performances. The symmetrical supercapacitor as assembled reaches a high energy density of 0.27 Wh cm −2 at the power density of 3.34 W cm −2 . By optimizing the composite structure, the energy barriers of the CuS/ZnO/PCc compound are reduced obviously in oxygen evolution reaction in comparison with pure PCc or CuS/PCc. The overpotential of the sample is decreased to 337 mV (10 mA cm −2 of the loop current) compared to that of PCc (492 mV) and CuS/PCc (415 mV). This work may inspire the rational design of composite electrode materials to achieve high performance in cross-field applications.

Ratiometric fluorescence assay based on carbon dots and Cu2+-catalyzed oxidation of O-phenylenediamine for the effective detection of deferasirox

Chen-Fang Miao,Xian-Zhong Guo,Xin-Tian Zhang,Yin-Ning Lin,Wen-Di Han,Zheng-Jun Huang,Shao-Huang Weng

The monitoring of deferasirox (DEF) has important clinical roles in patients who need iron excretion. However, analytical methods with practicability and simplicity are limited. Moreover, ratiometric fluorescence strategies based on Förster resonance energy transfer (FRET) from carbon dots (CDs) as a donor are rarely reported as a drug monitor. In this work, CDs with an appropriate emitting wavelength at 480 nm and excitation around 370 nm were prepared by hydrothermal approach and HCl post-treatment. O-Phenylenediamine (OPD) can be oxidized by Cu2+ to produce yellow fluorescent 2,3-diaminophenazine (oxOPD) in the system of Cu2+ and OPD (Cu–OPD). Correspondingly, a remarkable FRET from CDs to oxOPD in the system of CDs, Cu2+ and OPD (CDs–Cu–OPD) was fabricated with the quenching illustration of CDs, but emitting property of oxOPD. Attributed to the chelation ability of DEF on Cu2+, the inhibitory effects of DEF on the Cu2+-triggered oxidative capability reduced the FRET system by the decreased oxOPD. Thus, the recovered CDs at F480 and decreased oxOPD at F560 were found through a ratiometric mode by the addition of DEF in CDs–Cu–OPD for the DEF assay. The FRET behavior of CDs and oxOPD in CDs–Cu–OPD was proved clearly through the calculation of the association constant, binding constant, number of binding sites, and the distance between the donor and acceptor. Furthermore, this ratiometric method exhibited promising analytical performance for DEF with the application in real samples. The implementation of this work expands the application field of CDs and OPD oxidation in drug monitoring, and even other biological analyses through ratiometric strategy.